FvH4_2g26610

prolyl-tRNA synthetase associated domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009587
Physical Location & Seq
Reverse (-)
21344261 .. 21348625
4365 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26610.t2

Sequence Viewer

Length: 948 bp
ATGGGTCTTTCCAAGGAACAGCTTCTTGCTCGCTTAAAGGAGCTTGAGATTGATTTTTCCCAGTATGATCATCCTGTTGTTTTGACAGTTGAGGCTCAGGCAAAATATGTTGGACATCTGGGAGGTGGACTCAGTAAGAATTTATTCTTGAAGGATAAGAAGAGTAGGTTCTATATTGTTTCCGCTTTGGCAGATACTAAAGTAGATCTGAAAGTTCTATCTGTGAGGCTTGGTTTGGGAAAAGGTGGTTTGAGAATGGCTCCTGAAGAAGCACTGGGAGAGATACTTCAGGTCCCACCGGGTTCTGTTACTCCTTTATCACTGGTGAACGAATCTGCGCGCAATGTCTCTCTGCTGTTGGATCATAGATTTAAAAGTCAGAAGTTTTGCTTCTTCCACCCGTTATCGAATGACATGTCAATCTCATTAACTACCAATGGTCTTGACAAGTTTCTCAAGTCAATTGAGAGAGACCCTTCATATGTTGACTTGGAGGCTAACCCATCAGTTGGGAAGGACCAACCTCCTGATCTTGCATCTCTGGTGCCATCGAGTTCTGTAGTATTGCCAGATCCCCCAGAGACAGCAGCTTCCTCAAAAGTTCCTACAGAAAACAAAGTTTCTGCGAGCAACAAGTCGACAAAAAGTGCAGCAAGCACTTCTAAGCCATCCGTCAGTGCTACAAAATCCCAGGATAAACCAGCCAACATAACAGATGTTGGGAAACTAGTTGATGATTTACTTGAGTCAACATCTTCTCTATTGCTGAAAGAGATTACGGAGAAGAACTTTAAGGAACATGGAGAAGGACTTGGAGAAGAGTTATCGAAGAAAATTAAAAGTCAACTAGCTGCAGAATTGCACAACACAGTTATGATATTCAAAAATACTGCATACACGGAAGGATTCCATGCTGGGATGCACTGTCGTCCAAAGCGTTCTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 tRNA_edit

Protein Analysis

316

Amino Acids

34.21

Weight (kDa)

8.31

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_edit PF04073 24 - 151 6.5e-28 Aminoacyl-tRNA editing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 544
AccB7I CCANNNNNTGG 1 cut(s) 509
AccI GTMKAC 1 cut(s) 638
AccII CGCG 1 cut(s) 340
AciI CCGC 1 cut(s) 183
AclWI GGATC 2 cut(s) 369, 566
AcsI RAATTY 1 cut(s) 139
AcuI CTGAAG 2 cut(s) 272, 285
AfiI CCNNNNNNNGG 1 cut(s) 509
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 2 cut(s) 151, 883
AhlI ACTAGT 1 cut(s) 727
AjnI CCWGG 1 cut(s) 690
AluBI AGCT 4 cut(s) 22, 43, 590, 851
AluI AGCT 4 cut(s) 22, 43, 590, 851
Alw26I GTCTC 3 cut(s) 352, 465, 575
AlwI GGATC 2 cut(s) 369, 566
ApeKI GCWGC 3 cut(s) 587, 650, 851
ApoI RAATTY 1 cut(s) 139
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
Asp700I GAANNNNTTC 2 cut(s) 21, 143
AspLEI GCGC 2 cut(s) 340, 342
AspS9I GGNCC 2 cut(s) 292, 517
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 1 cut(s) 337
AvaII GGWCC 2 cut(s) 292, 517
BaeI ACNNNNGTAYC 2 cut(s) 186, 219
BanI GGYRCC 1 cut(s) 544
BbvI GCAGC 3 cut(s) 599, 662, 838
BccI CCATC 3 cut(s) 511, 556, 676
BciT130I CCWGG 1 cut(s) 692
BclI TGATCA 1 cut(s) 67
BcnI CCSGG 1 cut(s) 300
BcoDI GTCTC 3 cut(s) 352, 465, 575
BcuI ACTAGT 1 cut(s) 727
BfaI CTAG 2 cut(s) 728, 848
BfmI CTRYAG 3 cut(s) 558, 606, 852
BglII AGATCT 1 cut(s) 205
BisI GCNGC 3 cut(s) 588, 651, 852
BlsI GCNGC 3 cut(s) 589, 652, 853
Bme1390I CCNGG 2 cut(s) 300, 692
Bme18I GGWCC 2 cut(s) 292, 517
BmgT120I GGNCC 2 cut(s) 292, 517
BmiI GGNNCC 3 cut(s) 261, 294, 546
BmrFI CCNGG 2 cut(s) 300, 692
BmrI ACTGGG 2 cut(s) 55, 284
BmsI GCATC 2 cut(s) 545, 909
BmuI ACTGGG 2 cut(s) 55, 284
BplI GAGNNNNNCTC 4 cut(s) 114, 146, 244, 276
Bpu10I CCTNAGC 1 cut(s) 96
BpuEI CTTGAG 3 cut(s) 65, 440, 764
BpuMI CCSGG 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 465
BsaJI CCNNGG 2 cut(s) 12, 690
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse1I ACTGG 3 cut(s) 61, 279, 327
Bse3DI GCAATG 1 cut(s) 349
BseBI CCWGG 1 cut(s) 692
BseDI CCNNGG 2 cut(s) 12, 690
BseGI GGATG 3 cut(s) 70, 668, 924
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 2 cut(s) 110, 145
BseNI ACTGG 3 cut(s) 61, 279, 327
BsePI GCGCGC 1 cut(s) 338
BseXI GCAGC 3 cut(s) 599, 662, 838
BseYI CCCAGC 1 cut(s) 914
BsgI GTGCAG 1 cut(s) 669
Bsh1236I CGCG 1 cut(s) 340
BshNI GGYRCC 1 cut(s) 544
BsiSI CCGG 1 cut(s) 299
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 3 cut(s) 352, 465, 575
BsmFI GGGAC 1 cut(s) 278
Bso31I GGTCTC 1 cut(s) 465
Bsp143I GATC 5 cut(s) 67, 205, 361, 529, 571
BspACI CCGC 1 cut(s) 183
BspCNI CTCAG 2 cut(s) 109, 144
BspFNI CGCG 1 cut(s) 340
BspLI GGNNCC 3 cut(s) 261, 294, 546
BspMAI CTGCAG 1 cut(s) 856
BspPI GGATC 2 cut(s) 369, 566
BspT107I GGYRCC 1 cut(s) 544
BspTNI GGTCTC 1 cut(s) 465
BsrDI GCAATG 1 cut(s) 349
BsrI ACTGG 3 cut(s) 61, 279, 327
BssECI CCNNGG 2 cut(s) 12, 690
BssHII GCGCGC 1 cut(s) 338
BssMI GATC 5 cut(s) 67, 205, 361, 529, 571
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 692
Bst4CI ACNGT 3 cut(s) 88, 871, 926
Bst6I CTCTTC 2 cut(s) 155, 813
BstC8I GCNNGC 4 cut(s) 31, 340, 628, 655
BstDEI CTNAG 3 cut(s) 96, 131, 663
BstF5I GGATG 3 cut(s) 70, 668, 924
BstFNI CGCG 1 cut(s) 340
BstHHI GCGC 2 cut(s) 340, 342
BstKTI GATC 5 cut(s) 70, 208, 364, 532, 574
BstMAI GTCTC 3 cut(s) 352, 465, 575
BstMBI GATC 5 cut(s) 67, 205, 361, 529, 571
BstNI CCWGG 1 cut(s) 692
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 2 cut(s) 298, 690
BstSFI CTRYAG 3 cut(s) 558, 606, 852
BstUI CGCG 1 cut(s) 340
BstV1I GCAGC 3 cut(s) 599, 662, 838
BstX2I RGATCY 2 cut(s) 205, 571
BstYI RGATCY 2 cut(s) 205, 571
BtsCI GGATG 3 cut(s) 70, 668, 924
BtsIMutI CAGTG 4 cut(s) 272, 320, 682, 922
Cac8I GCNNGC 4 cut(s) 31, 340, 628, 655
CfoI GCGC 2 cut(s) 340, 342
Cfr13I GGNCC 2 cut(s) 292, 517
CviAII CATG 3 cut(s) 415, 800, 911
DdeI CTNAG 3 cut(s) 96, 131, 663
DpnI GATC 5 cut(s) 69, 207, 363, 531, 573
DpnII GATC 5 cut(s) 67, 205, 361, 529, 571
DraI TTTAAA 1 cut(s) 373
Eam1104I CTCTTC 2 cut(s) 155, 813
EarI CTCTTC 2 cut(s) 155, 813
Eco130I CCWWGG 1 cut(s) 12
Eco31I GGTCTC 1 cut(s) 465
Eco47I GGWCC 2 cut(s) 292, 517
Eco57I CTGAAG 2 cut(s) 272, 285
EcoO109I RGGNCCY 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 690
EcoT14I CCWWGG 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 3 cut(s) 418, 803, 914
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FaqI GGGAC 1 cut(s) 278
FatI CATG 3 cut(s) 414, 799, 910
FauNDI CATATG 1 cut(s) 481
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 638
Fnu4HI GCNGC 3 cut(s) 588, 651, 852
FokI GGATG 3 cut(s) 57, 655, 931
Fsp4HI GCNGC 3 cut(s) 588, 651, 852
FspBI CTAG 2 cut(s) 728, 848
GlaI GCGC 2 cut(s) 339, 341
GluI GCNGC 3 cut(s) 588, 651, 852
GsaI CCCAGC 1 cut(s) 918
HapII CCGG 1 cut(s) 299
HhaI GCGC 2 cut(s) 340, 342
Hin1II CATG 3 cut(s) 418, 803, 914
Hin6I GCGC 2 cut(s) 338, 340
HinP1I GCGC 2 cut(s) 338, 340
HincII GTYRAC 4 cut(s) 487, 639, 750, 845
HindII GTYRAC 4 cut(s) 487, 639, 750, 845
HinfI GANTC 4 cut(s) 129, 332, 746, 906
HpaII CCGG 1 cut(s) 299
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 6 cut(s) 128, 328, 487, 639, 750, 845
Hpy188I TCNGA 2 cut(s) 210, 381
Hpy188III TCNNGA 4 cut(s) 148, 263, 443, 527
Hpy8I GTNNAC 6 cut(s) 128, 328, 487, 639, 750, 845
HpyAV CCTTC 5 cut(s) 145, 486, 508, 800, 896
HpyCH4III ACNGT 3 cut(s) 88, 871, 926
HpyCH4V TGCA 6 cut(s) 536, 650, 854, 862, 893, 922
HpyF3I CTNAG 3 cut(s) 96, 131, 663
Hsp92II CATG 3 cut(s) 418, 803, 914
HspAI GCGC 2 cut(s) 338, 340
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 5 cut(s) 67, 205, 361, 529, 571
LmnI GCTCC 2 cut(s) 40, 265
Lsp1109I GCAGC 3 cut(s) 599, 662, 838
LweI GCATC 2 cut(s) 545, 909
MaeI CTAG 2 cut(s) 728, 848
MaeIII GTNAC 1 cut(s) 307
MalI GATC 5 cut(s) 69, 207, 363, 531, 573
MboI GATC 5 cut(s) 67, 205, 361, 529, 571
MboII GAAGA 7 cut(s) 172, 278, 385, 747, 796, 830, 841
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 2 cut(s) 205, 571
MluCI AATT 4 cut(s) 139, 462, 834, 857
MlyI GAGTC 2 cut(s) 123, 755
MmeI TCCRAC 2 cut(s) 91, 339
MnlI CCTC 6 cut(s) 85, 116, 219, 487, 534, 604
MroXI GAANNNNTTC 2 cut(s) 21, 143
MseI TTAA 5 cut(s) 35, 372, 428, 792, 837
MslI CAYNNNNRTG 1 cut(s) 872
MspI CCGG 1 cut(s) 299
MspR9I CCNGG 2 cut(s) 300, 692
MunI CAATTG 1 cut(s) 462
MvaI CCWGG 1 cut(s) 692
MvnI CGCG 1 cut(s) 340
NciI CCSGG 1 cut(s) 300
NdeI CATATG 1 cut(s) 481
NdeII GATC 5 cut(s) 67, 205, 361, 529, 571
NlaIII CATG 3 cut(s) 418, 803, 914
NlaIV GGNNCC 3 cut(s) 261, 294, 546
NspI RCATGY 1 cut(s) 418
PauI GCGCGC 1 cut(s) 338
PciI ACATGT 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 934
PdmI GAANNNNTTC 2 cut(s) 21, 143
PfeI GAWTC 2 cut(s) 332, 906
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 3 cut(s) 589, 652, 853
PleI GAGTC 2 cut(s) 123, 754
PpsI GAGTC 2 cut(s) 123, 754
PpuMI RGGWCCY 1 cut(s) 292
PscI ACATGT 1 cut(s) 414
Psp5II RGGWCCY 1 cut(s) 292
Psp6I CCWGG 1 cut(s) 690
PspFI CCCAGC 1 cut(s) 914
PspGI CCWGG 1 cut(s) 690
PspN4I GGNNCC 3 cut(s) 261, 294, 546
PspPI GGNCC 2 cut(s) 292, 517
PspPPI RGGWCCY 1 cut(s) 292
PstI CTGCAG 1 cut(s) 856
PsuI RGATCY 2 cut(s) 205, 571
PteI GCGCGC 1 cut(s) 338
RseI CAYNNNNRTG 1 cut(s) 872
SalI GTCGAC 1 cut(s) 637
SaqAI TTAA 5 cut(s) 35, 372, 428, 792, 837
SatI GCNGC 3 cut(s) 588, 651, 852
Sau3AI GATC 5 cut(s) 67, 205, 361, 529, 571
Sau96I GGNCC 2 cut(s) 292, 517
SchI GAGTC 2 cut(s) 123, 755
ScrFI CCNGG 2 cut(s) 300, 692
SetI ASST 9 cut(s) 24, 45, 127, 170, 247, 294, 526, 592, 853
SfaNI GCATC 2 cut(s) 545, 909
SfcI CTRYAG 3 cut(s) 558, 606, 852
SinI GGWCC 2 cut(s) 292, 517
SmiMI CAYNNNNRTG 1 cut(s) 872
SmlI CTYRAG 3 cut(s) 44, 455, 743
SmoI CTYRAG 3 cut(s) 44, 455, 743
SpeI ACTAGT 1 cut(s) 727
Sse9I AATT 4 cut(s) 139, 462, 834, 857
SsiI CCGC 1 cut(s) 183
SspMI CTAG 2 cut(s) 728, 848
StyD4I CCNGG 2 cut(s) 298, 690
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 3 cut(s) 88, 871, 926
TaqI TCGA 4 cut(s) 407, 551, 638, 827
TasI AATT 4 cut(s) 139, 462, 834, 857
TfiI GAWTC 2 cut(s) 332, 906
Tru1I TTAA 5 cut(s) 35, 372, 428, 792, 837
Tru9I TTAA 5 cut(s) 35, 372, 428, 792, 837
TscAI CASTG 4 cut(s) 279, 327, 682, 929
TseI GCWGC 3 cut(s) 587, 650, 851
TspDTI ATGAA 1 cut(s) 468
TspGWI ACGGA 3 cut(s) 661, 794, 914
TspRI CASTG 4 cut(s) 279, 327, 682, 929
Van91I CCANNNNNTGG 1 cut(s) 509
VpaK11BI GGWCC 2 cut(s) 292, 517
XapI RAATTY 1 cut(s) 139
XceI RCATGY 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 638
XmnI GAANNNNTTC 2 cut(s) 21, 143
XspI CTAG 2 cut(s) 728, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.