Prupe.1G501000_v2.0.a1

prolyl-tRNA synthetase associated domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0009587
Physical Location & Seq
Forward (+)
41466780 .. 41471274
4495 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G501000.2

Sequence Viewer

Length: 969 bp
ATGGGTCTTTCCAAGGAACAGTTGCTTGCTCGTTTAAAGGAGCTTCAAATTGGTTTTTCCCAGTATGAACATCCTGTTGTTTTGACAGTTGAGGCCCAGGCGAAATATGTTGGACATCTGGGAGGTGGACTGAGTAAAAATTTATTCTTGAAGGACAAGAAGAACAGGTTCTATATTGTTTCCGCTTTGGCAGATACCAAAGTAGATCTGAAAGTTCTATCTATAAGGCTTGGTTTGGGAAAAGGTGGTTTGAGAATGGCTCCTGAAGAAGCACTCGGAGAATTACTTCAGGTCCCACTGGGTTGTGTTACTCCGTTTTCGCTGGTGAATGAATCTGCACGGCATGTCTCTCTGTTGTTGGATCAAAAATTTAAAAGTCAGGAACATTGCTTCTTCCACCCATTGTCGAATGACACATCAATCTCACTTAGCACCCATGATCTCGATAAGTTCCTTAAATCAATTGGGAGAGACCCCTCATATGTTGACTTGGAGGCTAACCCTTCAGTTGGGAAAGATCAACCGCCAGATCTTGCTTCTCTTGTTCCATCTGGTTCCACAGTGTTGCCTGATCCTCCAGAAGCAGCAGCTTCCTCAAAAGATTCCATCGAAAATCCAGTTTCGGTCAGTAACAAGTCAACGACAAGTACAGCAAATGCTACTAAGCCATCCACTAATGCAAAAAATACTAAGGACAAACCAGTCAATGGTGTTCATGTATCAAAGTCCGTAACAGATGTTGGAAAGTTGGTTGAGGATTTACTGGATAAAACATCTGCATTACTGCTTTCAGAGACCACTGAGGAGACTATTAAGAAACATGGAGCACAGCTGGGAGCTGCGATATCAAATAGTATCAGAAGCCATCTTCGTGCAGAGTTGGAGAGCACCATTATGACGTTCAAAAACTCAGCATATACTGAAGGGTTTCATGCTGGTGTGCACCATCATGCAAAGCGTACATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 tRNA_edit

Protein Analysis

323

Amino Acids

34.76

Weight (kDa)

7.81

Isoelectric Point (pI)

26.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 701
AccB7I CCANNNNNTGG 1 cut(s) 707
AciI CCGC 2 cut(s) 183, 524
AclWI GGATC 2 cut(s) 369, 566
AcsI RAATTY 2 cut(s) 139, 368
AcuI CTGAAG 4 cut(s) 272, 285, 489, 942
AfaI GTAC 2 cut(s) 649, 961
AfiI CCNNNNNNNGG 2 cut(s) 509, 707
AgsI TTSAA 3 cut(s) 47, 151, 904
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 4 cut(s) 43, 590, 832, 839
AluI AGCT 4 cut(s) 43, 590, 832, 839
Alw21I GWGCWC 3 cut(s) 829, 890, 945
Alw26I GTCTC 4 cut(s) 352, 465, 788, 800
Alw44I GTGCAC 1 cut(s) 941
AlwI GGATC 2 cut(s) 369, 566
AoxI GGCC 1 cut(s) 93
ApaLI GTGCAC 1 cut(s) 941
ApeKI GCWGC 3 cut(s) 584, 587, 839
ApoI RAATTY 2 cut(s) 139, 368
Asp700I GAANNNNTTC 3 cut(s) 167, 285, 927
AspS9I GGNCC 2 cut(s) 94, 292
AsuHPI GGTGA 1 cut(s) 337
AvaII GGWCC 1 cut(s) 292
BaeGI GKGCMC 1 cut(s) 945
BaeI ACNNNNGTAYC 2 cut(s) 186, 219
Bbv12I GWGCWC 3 cut(s) 829, 890, 945
BbvI GCAGC 3 cut(s) 596, 599, 826
BccI CCATC 5 cut(s) 556, 614, 676, 873, 954
BceAI ACGGC 1 cut(s) 356
BciT130I CCWGG 1 cut(s) 98
BcoDI GTCTC 4 cut(s) 352, 465, 788, 800
BglII AGATCT 2 cut(s) 205, 529
BisI GCNGC 3 cut(s) 585, 588, 840
BlsI GCNGC 3 cut(s) 586, 589, 841
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 2 cut(s) 94, 292
BmiI GGNNCC 3 cut(s) 261, 294, 556
BmrFI CCNGG 1 cut(s) 98
BmrI ACTGGG 2 cut(s) 55, 308
BmuI ACTGGG 2 cut(s) 55, 308
BplI GAGNNNNNCTC 2 cut(s) 244, 276
BpmI CTGGAG 1 cut(s) 561
BsaI GGTCTC 2 cut(s) 465, 788
BsaJI CCNNGG 2 cut(s) 12, 96
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 707
Bse1I ACTGG 5 cut(s) 61, 303, 617, 701, 768
Bse3DI GCAATG 1 cut(s) 385
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 12, 96
BseGI GGATG 2 cut(s) 70, 668
BseLI CCNNNNNNNGG 2 cut(s) 509, 707
BseMI GCAATG 1 cut(s) 385
BseMII CTCAG 3 cut(s) 122, 792, 924
BseNI ACTGG 5 cut(s) 61, 303, 617, 701, 768
BseRI GAGGAG 1 cut(s) 818
BseSI GKGCMC 1 cut(s) 945
BseXI GCAGC 3 cut(s) 596, 599, 826
BseYI CCCAGC 1 cut(s) 832
BsgI GTGCAG 2 cut(s) 321, 894
BshFI GGCC 1 cut(s) 95
BsiHKAI GWGCWC 3 cut(s) 829, 890, 945
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 2 cut(s) 509, 707
BsmAI GTCTC 4 cut(s) 352, 465, 788, 800
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 1 cut(s) 95
Bso31I GGTCTC 2 cut(s) 465, 788
Bsp1286I GDGCHC 3 cut(s) 829, 890, 945
Bsp143I GATC 6 cut(s) 205, 361, 439, 517, 529, 571
BspACI CCGC 2 cut(s) 183, 524
BspANI GGCC 1 cut(s) 95
BspCNI CTCAG 3 cut(s) 123, 793, 923
BspLI GGNNCC 3 cut(s) 261, 294, 556
BspPI GGATC 2 cut(s) 369, 566
BspTNI GGTCTC 2 cut(s) 465, 788
BsrDI GCAATG 1 cut(s) 385
BsrI ACTGG 5 cut(s) 61, 303, 617, 701, 768
BssECI CCNNGG 2 cut(s) 12, 96
BssMI GATC 6 cut(s) 205, 361, 439, 517, 529, 571
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 3 cut(s) 21, 88, 562
BstC8I GCNNGC 1 cut(s) 27
BstDEI CTNAG 6 cut(s) 131, 428, 663, 690, 801, 910
BstF5I GGATG 2 cut(s) 70, 668
BstKTI GATC 6 cut(s) 208, 364, 442, 520, 532, 574
BstMAI GTCTC 4 cut(s) 352, 465, 788, 800
BstMBI GATC 6 cut(s) 205, 361, 439, 517, 529, 571
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 347
BstSCI CCNGG 1 cut(s) 96
BstSLI GKGCMC 1 cut(s) 945
BstV1I GCAGC 3 cut(s) 596, 599, 826
BstX2I RGATCY 2 cut(s) 205, 529
BstYI RGATCY 2 cut(s) 205, 529
BsuRI GGCC 1 cut(s) 95
BtsCI GGATG 2 cut(s) 70, 668
BtsIMutI CAGTG 3 cut(s) 296, 567, 798
Cac8I GCNNGC 1 cut(s) 27
Cfr13I GGNCC 2 cut(s) 94, 292
Csp6I GTAC 2 cut(s) 648, 960
CviAII CATG 6 cut(s) 344, 437, 716, 821, 932, 950
CviQI GTAC 2 cut(s) 648, 960
DdeI CTNAG 6 cut(s) 131, 428, 663, 690, 801, 910
DpnI GATC 6 cut(s) 207, 363, 441, 519, 531, 573
DpnII GATC 6 cut(s) 205, 361, 439, 517, 529, 571
DraI TTTAAA 2 cut(s) 36, 373
DrdI GACNNNNNNGTC 1 cut(s) 701
DseDI GACNNNNNNGTC 1 cut(s) 701
Eco130I CCWWGG 1 cut(s) 12
Eco31I GGTCTC 2 cut(s) 465, 788
Eco32I GATATC 1 cut(s) 846
Eco47I GGWCC 1 cut(s) 292
Eco57I CTGAAG 4 cut(s) 272, 285, 489, 942
EcoO109I RGGNCCY 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 96
EcoRV GATATC 1 cut(s) 846
EcoT14I CCWWGG 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 6 cut(s) 347, 440, 719, 824, 935, 953
FaqI GGGAC 1 cut(s) 278
FatI CATG 6 cut(s) 343, 436, 715, 820, 931, 949
FauNDI CATATG 1 cut(s) 481
Fnu4HI GCNGC 3 cut(s) 585, 588, 840
FokI GGATG 2 cut(s) 57, 655
Fsp4HI GCNGC 3 cut(s) 585, 588, 840
GluI GCNGC 3 cut(s) 585, 588, 840
GsaI CCCAGC 1 cut(s) 836
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 1 cut(s) 95
Hin1II CATG 6 cut(s) 347, 440, 719, 824, 935, 953
HincII GTYRAC 2 cut(s) 487, 639
HindII GTYRAC 2 cut(s) 487, 639
HinfI GANTC 2 cut(s) 332, 602
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 4 cut(s) 128, 487, 639, 943
Hpy188I TCNGA 4 cut(s) 210, 278, 793, 860
Hpy188III TCNNGA 5 cut(s) 148, 263, 380, 443, 578
Hpy8I GTNNAC 4 cut(s) 128, 487, 639, 943
HpyAV CCTTC 3 cut(s) 145, 513, 917
HpyCH4III ACNGT 3 cut(s) 21, 88, 562
HpyCH4IV ACGT 1 cut(s) 899
HpyCH4V TGCA 6 cut(s) 338, 680, 779, 875, 943, 953
HpyF3I CTNAG 6 cut(s) 131, 428, 663, 690, 801, 910
HpySE526I ACGT 1 cut(s) 899
Hsp92II CATG 6 cut(s) 347, 440, 719, 824, 935, 953
Kzo9I GATC 6 cut(s) 205, 361, 439, 517, 529, 571
LmnI GCTCC 4 cut(s) 40, 265, 824, 836
Lsp1109I GCAGC 3 cut(s) 596, 599, 826
MaeII ACGT 1 cut(s) 899
MaeIII GTNAC 3 cut(s) 307, 629, 730
MalI GATC 6 cut(s) 207, 363, 441, 519, 531, 573
MboI GATC 6 cut(s) 205, 361, 439, 517, 529, 571
MboII GAAGA 4 cut(s) 172, 278, 385, 860
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 2 cut(s) 205, 529
MhlI GDGCHC 3 cut(s) 829, 890, 945
MluCI AATT 5 cut(s) 48, 139, 281, 368, 462
MmeI TCCRAC 4 cut(s) 91, 339, 721, 861
MnlI CCTC 8 cut(s) 85, 116, 487, 487, 585, 604, 748, 796
MroXI GAANNNNTTC 3 cut(s) 167, 285, 927
MseI TTAA 4 cut(s) 35, 372, 456, 813
MslI CAYNNNNRTG 4 cut(s) 870, 893, 936, 948
MspA1I CMGCKG 1 cut(s) 832
MspR9I CCNGG 1 cut(s) 98
MunI CAATTG 1 cut(s) 462
MvaI CCWGG 1 cut(s) 98
NdeI CATATG 1 cut(s) 481
NdeII GATC 6 cut(s) 205, 361, 439, 517, 529, 571
NlaIII CATG 6 cut(s) 347, 440, 719, 824, 935, 953
NlaIV GGNNCC 3 cut(s) 261, 294, 556
NspI RCATGY 1 cut(s) 347
PdmI GAANNNNTTC 3 cut(s) 167, 285, 927
PfeI GAWTC 2 cut(s) 332, 602
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 3 cut(s) 586, 589, 841
PpuMI RGGWCCY 1 cut(s) 292
Psp5II RGGWCCY 1 cut(s) 292
Psp6I CCWGG 1 cut(s) 96
PspFI CCCAGC 1 cut(s) 832
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 3 cut(s) 261, 294, 556
PspPI GGNCC 2 cut(s) 94, 292
PspPPI RGGWCCY 1 cut(s) 292
PsuI RGATCY 2 cut(s) 205, 529
PvuII CAGCTG 1 cut(s) 832
RsaI GTAC 2 cut(s) 649, 961
RsaNI GTAC 2 cut(s) 648, 960
RseI CAYNNNNRTG 4 cut(s) 870, 893, 936, 948
SaqAI TTAA 4 cut(s) 35, 372, 456, 813
SatI GCNGC 3 cut(s) 585, 588, 840
Sau3AI GATC 6 cut(s) 205, 361, 439, 517, 529, 571
Sau96I GGNCC 2 cut(s) 94, 292
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 3 cut(s) 829, 890, 945
SetI ASST 9 cut(s) 45, 127, 170, 247, 294, 592, 834, 841, 902
SinI GGWCC 1 cut(s) 292
SmiMI CAYNNNNRTG 4 cut(s) 870, 893, 936, 948
Sse9I AATT 5 cut(s) 48, 139, 281, 368, 462
SsiI CCGC 2 cut(s) 183, 524
StyD4I CCNGG 1 cut(s) 96
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 3 cut(s) 21, 88, 562
TaiI ACGT 1 cut(s) 902
TaqI TCGA 3 cut(s) 407, 444, 609
TaqII GACCGA 1 cut(s) 613
TasI AATT 5 cut(s) 48, 139, 281, 368, 462
TatI WGTACW 1 cut(s) 647
TfiI GAWTC 2 cut(s) 332, 602
Tru1I TTAA 4 cut(s) 35, 372, 456, 813
Tru9I TTAA 4 cut(s) 35, 372, 456, 813
TscAI CASTG 3 cut(s) 303, 567, 805
TseI GCWGC 3 cut(s) 584, 587, 839
TspDTI ATGAA 4 cut(s) 81, 345, 704, 920
TspGWI ACGGA 2 cut(s) 303, 718
TspRI CASTG 3 cut(s) 303, 567, 805
Van91I CCANNNNNTGG 1 cut(s) 707
VneI GTGCAC 1 cut(s) 941
VpaK11BI GGWCC 1 cut(s) 292
XapI RAATTY 2 cut(s) 139, 368
XceI RCATGY 1 cut(s) 347
XmnI GAANNNNTTC 3 cut(s) 167, 285, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.