Rroxscaffold_7G00172450

prolyl-tRNA synthetase associated domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009587
Physical Location & Seq
Forward (+)
12690286 .. 12694291
4006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00172450.1

Sequence Viewer

Length: 1008 bp
ATGGGTCTTTCCAAGGAGCAGTTGCTTGCCCGCTTAAAAGGTCTTACCTTTACCTCAATCTCTCTCCCTTCATCTGTGTTTTATACAGTGTCTTTCTGTGAGCTTCAGATTGATTTTTCCCAGTATGATCATCCTGTTGTTTTGACAGTTGAGGCTCAGGCAAAATATGTTGGACATCTGGGAGGTGGACTCAGTAAAAATTTGTTCTTGAAGGATAAGAAGAGTAGGTTCTATATTGTTTCTGCTTTGGCAGATACTAAAGTAGATCTGAAAGCAGTTCTATCTGTGAGGCTTGGTTTGGGAAAAGGTGGCTTGAGAATGGCTCCTGAAGAAGCACTGGGAGAAATACTTCAGGTCCCATTGGGTTGTGTTACTCCTTTATCACTGGTGAACAAATCTGCATGCAATGTCTCTCTGCTGTTGGATCAAAAATTTAAAAGTCAGGAGTTTTGCTTCTTCCACCCATTATCGAATGACATGTCAATCTCACTAACCACCAATGGTCTCGACAAGTTTCTTAAGTCAATTAAGAGAGACCCCTCATATGTTGACTTGGAGGCTAACCCATCGGTTGGGAAGGACCAACCTCCTGATCTTGCATCTCTTGTTCCATCTAGTTCCGTAGTATTGCCAGATCCCCCGGAAACAGCAGCTTCCTCGAAAGATTGCACAGAAAATACAGTTTCTGTGGGTAACAAGTCCGCAAAAAGTACAGTGAGCACTTCTAAGCCATCCATTAATGCTAAAAAAACCCAGGATAAACCAGCCAACATAACAGATGTTGGAAAAATGGTTGATGACTTACTGGATTCAACATCTTCGCTAGTGCTGAAAGAGATTACGGAGGAGAATATTAAGGAACATGGAGAAGAGCTTGGAGAATTGTTGTCAAAGAAAATTAAAAATCAATTAGCTGCAGAATTGCACAACAGGATTATGATATTCAAAAACACTGCATACACAGAAGGATTCCACGCTGGGATGCACTGTCATCCAAAGCGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 tRNA_edit

Protein Analysis

335

Amino Acids

36.39

Weight (kDa)

7.62

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_edit PF04073 44 - 172 1.4e-24 Aminoacyl-tRNA editing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 572
AciI CCGC 2 cut(s) 31, 702
AclWI GGATC 2 cut(s) 432, 629
AcsI RAATTY 2 cut(s) 199, 431
AcuI CTGAAG 3 cut(s) 89, 335, 348
AfaI GTAC 1 cut(s) 712
AfiI CCNNNNNNNGG 1 cut(s) 572
AflII CTTAAG 1 cut(s) 518
AflIII ACRYGT 1 cut(s) 477
AgsI TTSAA 3 cut(s) 211, 813, 946
AjnI CCWGG 1 cut(s) 753
AluBI AGCT 4 cut(s) 103, 653, 874, 914
AluI AGCT 4 cut(s) 103, 653, 874, 914
Alw21I GWGCWC 1 cut(s) 722
Alw26I GTCTC 3 cut(s) 415, 509, 528
AlwI GGATC 2 cut(s) 432, 629
AlwNI CAGNNNCTG 1 cut(s) 686
ApeKI GCWGC 2 cut(s) 650, 914
ApoI RAATTY 2 cut(s) 199, 431
ArsI GACNNNNNNTTYG 2 cut(s) 464, 496
AseI ATTAAT 1 cut(s) 738
Asp700I GAANNNNTTC 1 cut(s) 348
AspS9I GGNCC 2 cut(s) 355, 580
AsuC2I CCSGG 1 cut(s) 641
AsuHPI GGTGA 1 cut(s) 400
AvaII GGWCC 2 cut(s) 355, 580
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
Bbv12I GWGCWC 1 cut(s) 722
BbvI GCAGC 2 cut(s) 662, 901
BccI CCATC 3 cut(s) 574, 619, 739
BcgI CGANNNNNNTGC 2 cut(s) 639, 673
BciT130I CCWGG 1 cut(s) 755
BclI TGATCA 1 cut(s) 127
BcnI CCSGG 1 cut(s) 641
BcoDI GTCTC 3 cut(s) 415, 509, 528
BfaI CTAG 2 cut(s) 615, 824
BfmI CTRYAG 1 cut(s) 915
BfrI CTTAAG 1 cut(s) 518
BglII AGATCT 1 cut(s) 265
BisI GCNGC 2 cut(s) 651, 915
BlsI GCNGC 2 cut(s) 652, 916
Bme1390I CCNGG 2 cut(s) 641, 755
Bme18I GGWCC 2 cut(s) 355, 580
BmgT120I GGNCC 2 cut(s) 355, 580
BmiI GGNNCC 2 cut(s) 324, 357
BmrFI CCNGG 2 cut(s) 641, 755
BmrI ACTGGG 2 cut(s) 115, 347
BmsI GCATC 2 cut(s) 608, 972
BmuI ACTGGG 2 cut(s) 115, 347
BplI GAGNNNNNCTC 4 cut(s) 174, 206, 307, 339
Bpu10I CCTNAGC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 334
BpuMI CCSGG 1 cut(s) 641
BsaI GGTCTC 2 cut(s) 509, 528
BsaJI CCNNGG 3 cut(s) 12, 639, 753
Bsc4I CCNNNNNNNGG 1 cut(s) 572
Bse1I ACTGG 4 cut(s) 121, 342, 390, 810
Bse3DI GCAATG 1 cut(s) 412
BseBI CCWGG 1 cut(s) 755
BseDI CCNNGG 3 cut(s) 12, 639, 753
BseGI GGATG 4 cut(s) 130, 731, 987, 991
BseLI CCNNNNNNNGG 1 cut(s) 572
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 2 cut(s) 170, 205
BseNI ACTGG 4 cut(s) 121, 342, 390, 810
BseRI GAGGAG 1 cut(s) 860
BseXI GCAGC 2 cut(s) 662, 901
BseYI CCCAGC 1 cut(s) 977
BsiHKAI GWGCWC 1 cut(s) 722
BsiSI CCGG 1 cut(s) 641
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 1 cut(s) 572
BsmAI GTCTC 3 cut(s) 415, 509, 528
BsmFI GGGAC 1 cut(s) 341
Bso31I GGTCTC 2 cut(s) 509, 528
Bsp1286I GDGCHC 1 cut(s) 722
Bsp143I GATC 5 cut(s) 127, 265, 424, 592, 634
BspACI CCGC 2 cut(s) 31, 702
BspCNI CTCAG 2 cut(s) 169, 204
BspLI GGNNCC 2 cut(s) 324, 357
BspMAI CTGCAG 1 cut(s) 919
BspPI GGATC 2 cut(s) 432, 629
BspQI GCTCTTC 1 cut(s) 864
BspTI CTTAAG 1 cut(s) 518
BspTNI GGTCTC 2 cut(s) 509, 528
BsrDI GCAATG 1 cut(s) 412
BsrI ACTGG 4 cut(s) 121, 342, 390, 810
BssECI CCNNGG 3 cut(s) 12, 639, 753
BssMI GATC 5 cut(s) 127, 265, 424, 592, 634
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 755
Bst4CI ACNGT 5 cut(s) 88, 148, 682, 715, 989
Bst6I CTCTTC 2 cut(s) 215, 864
BstAFI CTTAAG 1 cut(s) 518
BstC8I GCNNGC 3 cut(s) 27, 31, 403
BstDEI CTNAG 4 cut(s) 156, 191, 726, 1005
BstF5I GGATG 4 cut(s) 130, 731, 987, 991
BstKTI GATC 5 cut(s) 130, 268, 427, 595, 637
BstMAI GTCTC 3 cut(s) 415, 509, 528
BstMBI GATC 5 cut(s) 127, 265, 424, 592, 634
BstNI CCWGG 1 cut(s) 755
BstNSI RCATGY 2 cut(s) 405, 481
BstSCI CCNGG 2 cut(s) 639, 753
BstSFI CTRYAG 1 cut(s) 915
BstV1I GCAGC 2 cut(s) 662, 901
BstX2I RGATCY 2 cut(s) 265, 634
BstYI RGATCY 2 cut(s) 265, 634
BtsCI GGATG 4 cut(s) 130, 731, 987, 991
BtsI GCAGTG 1 cut(s) 951
BtsIMutI CAGTG 6 cut(s) 93, 335, 383, 720, 951, 985
Cac8I GCNNGC 3 cut(s) 27, 31, 403
CaiI CAGNNNCTG 1 cut(s) 686
Cfr13I GGNCC 2 cut(s) 355, 580
Csp6I GTAC 1 cut(s) 711
CviAII CATG 3 cut(s) 402, 478, 863
CviQI GTAC 1 cut(s) 711
DdeI CTNAG 4 cut(s) 156, 191, 726, 1005
DpnI GATC 5 cut(s) 129, 267, 426, 594, 636
DpnII GATC 5 cut(s) 127, 265, 424, 592, 634
DraI TTTAAA 1 cut(s) 436
Eam1104I CTCTTC 2 cut(s) 215, 864
EarI CTCTTC 2 cut(s) 215, 864
Eco130I CCWWGG 1 cut(s) 12
Eco31I GGTCTC 2 cut(s) 509, 528
Eco47I GGWCC 2 cut(s) 355, 580
Eco57I CTGAAG 3 cut(s) 89, 335, 348
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 1 cut(s) 753
EcoT14I CCWWGG 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 3 cut(s) 405, 481, 866
FaqI GGGAC 1 cut(s) 341
FatI CATG 3 cut(s) 401, 477, 862
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 1 cut(s) 544
FbaI TGATCA 1 cut(s) 127
Fnu4HI GCNGC 2 cut(s) 651, 915
FokI GGATG 4 cut(s) 117, 718, 978, 994
Fsp4HI GCNGC 2 cut(s) 651, 915
FspBI CTAG 2 cut(s) 615, 824
GluI GCNGC 2 cut(s) 651, 915
GsaI CCCAGC 1 cut(s) 981
HapII CCGG 1 cut(s) 641
Hin1II CATG 3 cut(s) 405, 481, 866
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HinfI GANTC 3 cut(s) 189, 809, 969
HpaII CCGG 1 cut(s) 641
HphI GGTGA 1 cut(s) 400
Hpy166II GTNNAC 3 cut(s) 188, 391, 550
Hpy188I TCNGA 2 cut(s) 108, 270
Hpy188III TCNNGA 5 cut(s) 208, 326, 443, 506, 590
Hpy8I GTNNAC 3 cut(s) 188, 391, 550
HpyAV CCTTC 4 cut(s) 78, 205, 571, 959
HpyCH4III ACNGT 5 cut(s) 88, 148, 682, 715, 989
HpyCH4V TGCA 8 cut(s) 401, 405, 599, 669, 917, 925, 956, 985
HpyF3I CTNAG 4 cut(s) 156, 191, 726, 1005
Hsp92II CATG 3 cut(s) 405, 481, 866
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 5 cut(s) 127, 265, 424, 592, 634
LguI GCTCTTC 1 cut(s) 864
LmnI GCTCC 2 cut(s) 16, 328
Lsp1109I GCAGC 2 cut(s) 662, 901
LweI GCATC 2 cut(s) 608, 972
MaeI CTAG 2 cut(s) 615, 824
MaeIII GTNAC 2 cut(s) 370, 692
MalI GATC 5 cut(s) 129, 267, 426, 594, 636
MboI GATC 5 cut(s) 127, 265, 424, 592, 634
MboII GAAGA 5 cut(s) 232, 341, 448, 810, 881
MflI RGATCY 2 cut(s) 265, 634
MhlI GDGCHC 1 cut(s) 722
MluCI AATT 7 cut(s) 199, 431, 525, 881, 897, 908, 920
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 3 cut(s) 151, 402, 763
MnlI CCTC 9 cut(s) 64, 145, 176, 282, 550, 550, 597, 667, 838
MroXI GAANNNNTTC 1 cut(s) 348
MseI TTAA 7 cut(s) 35, 435, 519, 528, 738, 855, 900
MspCI CTTAAG 1 cut(s) 518
MspI CCGG 1 cut(s) 641
MspR9I CCNGG 2 cut(s) 641, 755
MvaI CCWGG 1 cut(s) 755
NciI CCSGG 1 cut(s) 641
NdeI CATATG 1 cut(s) 544
NdeII GATC 5 cut(s) 127, 265, 424, 592, 634
NlaIII CATG 3 cut(s) 405, 481, 866
NlaIV GGNNCC 2 cut(s) 324, 357
NspI RCATGY 2 cut(s) 405, 481
PaeI GCATGC 1 cut(s) 405
PciI ACATGT 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 864
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 2 cut(s) 809, 969
PflMI CCANNNNNTGG 1 cut(s) 572
PkrI GCNGC 2 cut(s) 652, 916
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
PpuMI RGGWCCY 1 cut(s) 355
PscI ACATGT 1 cut(s) 477
PshBI ATTAAT 1 cut(s) 738
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 753
PspFI CCCAGC 1 cut(s) 977
PspGI CCWGG 1 cut(s) 753
PspN4I GGNNCC 2 cut(s) 324, 357
PspPI GGNCC 2 cut(s) 355, 580
PspPPI RGGWCCY 1 cut(s) 355
PstI CTGCAG 1 cut(s) 919
PstNI CAGNNNCTG 1 cut(s) 686
PsuI RGATCY 2 cut(s) 265, 634
RsaI GTAC 1 cut(s) 712
RsaNI GTAC 1 cut(s) 711
SapI GCTCTTC 1 cut(s) 864
SaqAI TTAA 7 cut(s) 35, 435, 519, 528, 738, 855, 900
SatI GCNGC 2 cut(s) 651, 915
Sau3AI GATC 5 cut(s) 127, 265, 424, 592, 634
Sau96I GGNCC 2 cut(s) 355, 580
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 2 cut(s) 641, 755
SduI GDGCHC 1 cut(s) 722
SfaNI GCATC 2 cut(s) 608, 972
SfcI CTRYAG 1 cut(s) 915
SinI GGWCC 2 cut(s) 355, 580
SmlI CTYRAG 2 cut(s) 313, 518
SmoI CTYRAG 2 cut(s) 313, 518
SphI GCATGC 1 cut(s) 405
Sse9I AATT 7 cut(s) 199, 431, 525, 881, 897, 908, 920
SsiI CCGC 2 cut(s) 31, 702
SspI AATATT 1 cut(s) 853
SspMI CTAG 2 cut(s) 615, 824
StyD4I CCNGG 2 cut(s) 639, 753
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 5 cut(s) 88, 148, 682, 715, 989
TaqI TCGA 3 cut(s) 470, 507, 659
TasI AATT 7 cut(s) 199, 431, 525, 881, 897, 908, 920
TatI WGTACW 1 cut(s) 710
TfiI GAWTC 2 cut(s) 809, 969
Tru1I TTAA 7 cut(s) 35, 435, 519, 528, 738, 855, 900
Tru9I TTAA 7 cut(s) 35, 435, 519, 528, 738, 855, 900
TscAI CASTG 6 cut(s) 93, 342, 390, 720, 958, 992
TseI GCWGC 2 cut(s) 650, 914
TspDTI ATGAA 1 cut(s) 60
TspGWI ACGGA 2 cut(s) 610, 857
TspRI CASTG 6 cut(s) 93, 342, 390, 720, 958, 992
Van91I CCANNNNNTGG 1 cut(s) 572
Vha464I CTTAAG 1 cut(s) 518
VpaK11BI GGWCC 2 cut(s) 355, 580
VspI ATTAAT 1 cut(s) 738
XapI RAATTY 2 cut(s) 199, 431
XceI RCATGY 2 cut(s) 405, 481
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 2 cut(s) 615, 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.