AT1G48400

positive regulation of bicellular tight junction assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005679
Physical Location & Seq
Reverse (-)
17881968 .. 17883967
2000 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G48400.2

Sequence Viewer

Length: 1542 bp
ATGGCTATGATGAGAACTTCTCCTAGAGATTCGATAAGCAATTTGCCAGATGAGATTCTTGGCAAAATCTTGTCTTTGCTCCCGACAAAAGTGGCTGCTTCCACATCGGTTCTGTCCAAGAGGTGGAGGAATCTGTTGGGTCTTGTAGACAACCTCTGCTTTGATGAGTCGATGGTTGTTTATCCCAACGAAGAAGAAGAAACAAGTGGTTCACTTCGCTTCTGTGATTTCGTAGACAAGACATTTGCTCTGTTAAGCAATTCTCACATCAAGAAGTTCTCTCTGTCTCGTGTATATAAGTATAACGATGATGTAGACGGTATGGTCCGGCGTTGGATTCGGACTGTAATGGAACGCGGTTTATTGGAAATACACTTGCACGCCACCCCCATGTCTTTTGTTGCCATAGAAACTAAGCTGTTAACGAGCAACACACTGGTTAAGCTCACACTATCCGCTCGATGTTTTGTTGAAGTAGAGCGTGTGTTTTTCCCTGCCCTCAAATCACTTTCTCTCTTCTCAATTCTGGGTGATTATACCAACTATATACGGCTCATCGATGGCTGCCCTGTGTTGGAAGAATTATACATGCGTGATGGTGATTATCCGTTCTTGCGGCTTACTTGTGGTACCAACGTGGAAAGTGCATCCCTCAAGCGACTTGTGATTTTTACCCATAATCCAAATGAAATGATTTGGCACGAACTGATTTATTTTGAAGCACCAAGTCTTGTCTACCTTGACTATTCCAGTTATGTCTCGGCTAAGTATGATGTTGTTGATTTCGATTTGCTTGTCGAAGCCAGGCTGAGTCTCAGGTTATGGGTGTCAACTAACGATTATGATTATTCTGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGATGGTGATTATTATATTGTAGAACCAAAGGCGCCTATATTTGGTGATGTTACAGAACTACTTGCGGCTATACGCAACATTACGACCCTTCACTTGTCTCCTGATTCCCTTGAGGTGTTTCATTTCTGCTGTAAATCCATGCCCGTGTTCAACAACCTCCTTAATTTATCTATTGAGAGTAACAAGGATAAAGGTTGGCAAGTAATGCCACTTCTCCTCAAGAGTTGTCCAAATCTACACACCTTAGTCATCAAGGGTCTTGTCCACAGAGTAACTAGTAGATGTGGAGATGCATGCGCTTGCATACCTAAGAAGCAGAGGAAGATTGTACAGAAAGAAGAGGCATTATGTTGTCTGCGGACATGTCAAGTGAAGGTGCTACAGATTTCAGAGTATGGAGGTTATTTTCAAGAGCTGAAACAGATGAGACATTTCTTGGGCAAGTTGGAATGTCTTGAAACCGTGAAAGTTGGTGTTCACGCAGAAAACAACAACAACAGTGAGTTCTTGCGAGCTAATGTGCTGACTCTTCCCAGAGTTTCAGCAAAGTGCAACGTCCACTTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001654 GO:0003006 GO:0003008 GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005815 GO:0005829 GO:0005856 GO:0005911 GO:0005923 GO:0005929 GO:0006996 GO:0007010 GO:0007154 GO:0007155 GO:0007165 GO:0007275 GO:0007276 GO:0007283 GO:0007399 GO:0007423 GO:0007568 GO:0007588 GO:0007610 GO:0007617 GO:0007618 GO:0007626 GO:0007632 GO:0008104 GO:0008150 GO:0008340 GO:0009314 GO:0009416 GO:0009628 GO:0009987 GO:0010259 GO:0015630 GO:0016020 GO:0016043 GO:0019098 GO:0019953 GO:0022008 GO:0022406 GO:0022412 GO:0022414 GO:0022607 GO:0022610 GO:0023041 GO:0023052 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030054 GO:0030154 GO:0030182 GO:0031514 GO:0032391 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0034606 GO:0034607 GO:0034613 GO:0035178 GO:0035845 GO:0035869 GO:0036064 GO:0042461 GO:0042995 GO:0043005 GO:0043010 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043296 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044703 GO:0044782 GO:0046530 GO:0048232 GO:0048468 GO:0048513 GO:0048515 GO:0048518 GO:0048522 GO:0048609 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051179 GO:0051640 GO:0051641 GO:0051704 GO:0051716 GO:0060041 GO:0060179 GO:0060271 GO:0065007 GO:0070160 GO:0070727 GO:0070925 GO:0071840 GO:0097458 GO:0097546 GO:0097711 GO:0097730 GO:0097731 GO:0097733 GO:0098609 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:0140056 GO:1901888 GO:1901890 GO:1903348 GO:1905515 GO:2000810
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box SH3_1

Protein Analysis

513

Amino Acids

58.62

Weight (kDa)

4.52

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 12 - 48 4.9e-08 F-box domain
LRR_At5g56370 PF24758 109 - 247 4.1e-10 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 323
Acc65I GGTACC 1 cut(s) 629
AccB1I GGYRCC 2 cut(s) 629, 1003
AccB7I CCANNNNNTGG 1 cut(s) 123
AccBSI CCGCTC 1 cut(s) 458
AccI GTMKAC 4 cut(s) 147, 234, 315, 735
AccII CGCG 1 cut(s) 357
AciI CCGC 5 cut(s) 357, 456, 616, 1037, 1330
AcyI GRCGYC 1 cut(s) 1004
AfaI GTAC 2 cut(s) 631, 1302
AfiI CCNNNNNNNGG 3 cut(s) 123, 574, 1013
AflIII ACRYGT 1 cut(s) 1334
AgsI TTSAA 5 cut(s) 473, 719, 1123, 1382, 1430
AhlI ACTAGT 1 cut(s) 1247
AjnI CCWGG 1 cut(s) 803
AluBI AGCT 4 cut(s) 418, 445, 1387, 1487
AluI AGCT 4 cut(s) 418, 445, 1387, 1487
Alw26I GTCTC 5 cut(s) 291, 763, 818, 1074, 1393
ApeKI GCWGC 2 cut(s) 95, 564
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
Asp718I GGTACC 1 cut(s) 629
AspLEI GCGC 2 cut(s) 1006, 1271
AspS9I GGNCC 1 cut(s) 325
AsuHPI GGTGA 4 cut(s) 542, 611, 989, 1028
AvaII GGWCC 1 cut(s) 325
BaeI ACNNNNGTAYC 2 cut(s) 621, 654
BanI GGYRCC 2 cut(s) 629, 1003
BarI GAAGNNNNNNTAC 2 cut(s) 1167, 1199
BauI CACGAG 1 cut(s) 288
BbvI GCAGC 2 cut(s) 82, 551
BccI CCATC 4 cut(s) 166, 554, 590, 968
BceAI ACGGC 1 cut(s) 566
BciT130I CCWGG 1 cut(s) 805
BcoDI GTCTC 5 cut(s) 291, 763, 818, 1074, 1393
BcuI ACTAGT 1 cut(s) 1247
BfaI CTAG 2 cut(s) 24, 1248
BfmI CTRYAG 1 cut(s) 1352
BfoI RGCGCY 1 cut(s) 1007
BisI GCNGC 4 cut(s) 96, 565, 617, 1038
BlsI GCNGC 4 cut(s) 97, 566, 618, 1039
Bme1390I CCNGG 1 cut(s) 805
Bme18I GGWCC 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 325
BmiI GGNNCC 2 cut(s) 631, 1005
BmrFI CCNGG 1 cut(s) 805
BmsI GCATC 2 cut(s) 656, 1252
BplI GAGNNNNNCTC 1 cut(s) 36
BpuEI CTTGAG 3 cut(s) 638, 1103, 1175
Bsa29I ATCGAT 1 cut(s) 558
BsaHI GRCGYC 1 cut(s) 1004
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 574, 1013
Bse1I ACTGG 2 cut(s) 441, 750
BseBI CCWGG 1 cut(s) 805
BseCI ATCGAT 1 cut(s) 558
BseGI GGATG 1 cut(s) 647
BseLI CCNNNNNNNGG 3 cut(s) 123, 574, 1013
BseMII CTCAG 2 cut(s) 800, 829
BseNI ACTGG 2 cut(s) 441, 750
BseRI GAGGAG 1 cut(s) 1178
BseXI GCAGC 2 cut(s) 82, 551
Bsh1236I CGCG 1 cut(s) 357
BshNI GGYRCC 2 cut(s) 629, 1003
BshVI ATCGAT 1 cut(s) 558
BsiSI CCGG 1 cut(s) 328
BslI CCNNNNNNNGG 3 cut(s) 123, 574, 1013
BsmAI GTCTC 5 cut(s) 291, 763, 818, 1074, 1393
Bsp1407I TGTACA 1 cut(s) 1300
BspACI CCGC 5 cut(s) 357, 456, 616, 1037, 1330
BspCNI CTCAG 2 cut(s) 801, 828
BspDI ATCGAT 1 cut(s) 558
BspFNI CGCG 1 cut(s) 357
BspLI GGNNCC 2 cut(s) 631, 1005
BspT107I GGYRCC 2 cut(s) 629, 1003
BsrBI CCGCTC 1 cut(s) 458
BsrGI TGTACA 1 cut(s) 1300
BsrI ACTGG 2 cut(s) 441, 750
BssNI GRCGYC 1 cut(s) 1004
BssSI CACGAG 1 cut(s) 288
Bst2BI CACGAG 1 cut(s) 288
Bst2UI CCWGG 1 cut(s) 805
Bst4CI ACNGT 4 cut(s) 320, 346, 1435, 1472
Bst6I CTCTTC 3 cut(s) 521, 1305, 1506
BstACI GRCGYC 1 cut(s) 1004
BstAPI GCANNNNNTGC 1 cut(s) 1177
BstAUI TGTACA 1 cut(s) 1300
BstC8I GCNNGC 4 cut(s) 381, 1267, 1273, 1485
BstDEI CTNAG 6 cut(s) 414, 765, 809, 815, 1216, 1281
BstF5I GGATG 1 cut(s) 647
BstFNI CGCG 1 cut(s) 357
BstH2I RGCGCY 1 cut(s) 1007
BstHHI GCGC 2 cut(s) 1006, 1271
BstMAI GTCTC 5 cut(s) 291, 763, 818, 1074, 1393
BstMWI GCNNNNNNNGC 1 cut(s) 1177
BstNI CCWGG 1 cut(s) 805
BstNSI RCATGY 3 cut(s) 592, 1269, 1338
BstSCI CCNGG 1 cut(s) 803
BstSFI CTRYAG 1 cut(s) 1352
BstUI CGCG 1 cut(s) 357
BstV1I GCAGC 2 cut(s) 82, 551
Bsu15I ATCGAT 1 cut(s) 558
BsuTUI ATCGAT 1 cut(s) 558
BtsCI GGATG 1 cut(s) 647
BtsIMutI CAGTG 2 cut(s) 434, 1477
Cac8I GCNNGC 4 cut(s) 381, 1267, 1273, 1485
CfoI GCGC 2 cut(s) 1006, 1271
Cfr13I GGNCC 1 cut(s) 325
ClaI ATCGAT 1 cut(s) 558
Csp6I GTAC 2 cut(s) 630, 1301
CviAII CATG 5 cut(s) 391, 589, 1111, 1266, 1335
CviQI GTAC 2 cut(s) 630, 1301
DdeI CTNAG 6 cut(s) 414, 765, 809, 815, 1216, 1281
DinI GGCGCC 1 cut(s) 1005
DrdI GACNNNNNNGTC 1 cut(s) 323
DseDI GACNNNNNNGTC 1 cut(s) 323
Eam1104I CTCTTC 3 cut(s) 521, 1305, 1506
EarI CTCTTC 3 cut(s) 521, 1305, 1506
Eco47I GGWCC 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 803
EcoT22I ATGCAT 1 cut(s) 1267
EgeI GGCGCC 1 cut(s) 1005
EheI GGCGCC 1 cut(s) 1005
FaeI CATG 5 cut(s) 394, 592, 1114, 1269, 1338
FatI CATG 5 cut(s) 390, 588, 1110, 1265, 1334
FblI GTMKAC 4 cut(s) 147, 234, 315, 735
Fnu4HI GCNGC 4 cut(s) 96, 565, 617, 1038
FokI GGATG 1 cut(s) 634
Fsp4HI GCNGC 4 cut(s) 96, 565, 617, 1038
FspBI CTAG 2 cut(s) 24, 1248
GlaI GCGC 2 cut(s) 1005, 1270
GluI GCNGC 4 cut(s) 96, 565, 617, 1038
HaeII RGCGCY 1 cut(s) 1007
HapII CCGG 1 cut(s) 328
HhaI GCGC 2 cut(s) 1006, 1271
Hin1I GRCGYC 1 cut(s) 1004
Hin1II CATG 5 cut(s) 394, 592, 1114, 1269, 1338
Hin6I GCGC 2 cut(s) 1004, 1269
HinP1I GCGC 2 cut(s) 1004, 1269
HincII GTYRAC 2 cut(s) 423, 831
HindII GTYRAC 2 cut(s) 423, 831
HinfI GANTC 8 cut(s) 29, 55, 130, 167, 337, 811, 1076, 1498
HpaI GTTAAC 1 cut(s) 423
HpaII CCGG 1 cut(s) 328
HphI GGTGA 4 cut(s) 542, 611, 989, 1028
Hpy188I TCNGA 4 cut(s) 342, 853, 1363, 1541
Hpy188III TCNNGA 6 cut(s) 82, 271, 1073, 1192, 1382, 1427
HpyAV CCTTC 2 cut(s) 1070, 1339
HpyCH4III ACNGT 4 cut(s) 320, 346, 1435, 1472
HpyCH4IV ACGT 2 cut(s) 636, 1527
HpyCH4V TGCA 5 cut(s) 379, 647, 1265, 1275, 1524
HpyF10VI GCNNNNNNNGC 1 cut(s) 1177
HpyF3I CTNAG 6 cut(s) 414, 765, 809, 815, 1216, 1281
HpySE526I ACGT 2 cut(s) 636, 1527
Hsp92I GRCGYC 1 cut(s) 1004
Hsp92II CATG 5 cut(s) 394, 592, 1114, 1269, 1338
HspAI GCGC 2 cut(s) 1004, 1269
KasI GGCGCC 1 cut(s) 1003
KpnI GGTACC 1 cut(s) 633
KspAI GTTAAC 1 cut(s) 423
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 2 cut(s) 82, 551
LweI GCATC 2 cut(s) 656, 1252
MaeI CTAG 2 cut(s) 24, 1248
MaeII ACGT 2 cut(s) 636, 1527
MaeIII GTNAC 3 cut(s) 1021, 1151, 1243
MbiI CCGCTC 1 cut(s) 458
MboII GAAGA 8 cut(s) 203, 206, 209, 508, 590, 1306, 1322, 1493
MluCI AATT 5 cut(s) 40, 259, 522, 581, 1135
Mly113I GGCGCC 1 cut(s) 1004
MlyI GAGTC 3 cut(s) 176, 820, 1492
MmeI TCCRAC 3 cut(s) 314, 555, 1398
Mph1103I ATGCAT 1 cut(s) 1267
MseI TTAA 4 cut(s) 254, 422, 441, 1134
MslI CAYNNNNRTG 2 cut(s) 389, 1115
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 1 cut(s) 805
MvaI CCWGG 1 cut(s) 805
MvnI CGCG 1 cut(s) 357
MwoI GCNNNNNNNGC 1 cut(s) 1177
NarI GGCGCC 1 cut(s) 1004
NlaIII CATG 5 cut(s) 394, 592, 1114, 1269, 1338
NlaIV GGNNCC 2 cut(s) 631, 1005
NmeAIII GCCGAG 1 cut(s) 740
NsiI ATGCAT 1 cut(s) 1267
NspI RCATGY 3 cut(s) 592, 1269, 1338
PaeI GCATGC 1 cut(s) 1269
PciI ACATGT 1 cut(s) 1334
PfeI GAWTC 5 cut(s) 29, 55, 130, 337, 1076
PflMI CCANNNNNTGG 1 cut(s) 123
PkrI GCNGC 4 cut(s) 97, 566, 618, 1039
PleI GAGTC 3 cut(s) 175, 819, 1492
PluTI GGCGCC 1 cut(s) 1007
PpsI GAGTC 3 cut(s) 175, 819, 1492
PscI ACATGT 1 cut(s) 1334
Psp6I CCWGG 1 cut(s) 803
PspGI CCWGG 1 cut(s) 803
PspN4I GGNNCC 2 cut(s) 631, 1005
PspPI GGNCC 1 cut(s) 325
RsaI GTAC 2 cut(s) 631, 1302
RsaNI GTAC 2 cut(s) 630, 1301
RseI CAYNNNNRTG 2 cut(s) 389, 1115
SaqAI TTAA 4 cut(s) 254, 422, 441, 1134
SatI GCNGC 4 cut(s) 96, 565, 617, 1038
Sau96I GGNCC 1 cut(s) 325
SchI GAGTC 3 cut(s) 176, 820, 1492
ScrFI CCNGG 1 cut(s) 805
SfaNI GCATC 2 cut(s) 656, 1252
SfcI CTRYAG 1 cut(s) 1352
SfoI GGCGCC 1 cut(s) 1005
SinI GGWCC 1 cut(s) 325
SmiMI CAYNNNNRTG 2 cut(s) 389, 1115
SmlI CTYRAG 3 cut(s) 653, 1082, 1190
SmoI CTYRAG 3 cut(s) 653, 1082, 1190
SpeI ACTAGT 1 cut(s) 1247
SphI GCATGC 1 cut(s) 1269
Sse9I AATT 5 cut(s) 40, 259, 522, 581, 1135
SsiI CCGC 5 cut(s) 357, 456, 616, 1037, 1330
SspDI GGCGCC 1 cut(s) 1003
SspMI CTAG 2 cut(s) 24, 1248
StyD4I CCNGG 1 cut(s) 803
TaaI ACNGT 4 cut(s) 320, 346, 1435, 1472
TaiI ACGT 2 cut(s) 639, 1530
TaqI TCGA 6 cut(s) 32, 170, 460, 558, 786, 798
TasI AATT 5 cut(s) 40, 259, 522, 581, 1135
TatI WGTACW 1 cut(s) 1300
TauI GCSGC 2 cut(s) 619, 1040
TfiI GAWTC 5 cut(s) 29, 55, 130, 337, 1076
Tru1I TTAA 4 cut(s) 254, 422, 441, 1134
Tru9I TTAA 4 cut(s) 254, 422, 441, 1134
TscAI CASTG 2 cut(s) 441, 1477
TseI GCWGC 2 cut(s) 95, 564
TspDTI ATGAA 3 cut(s) 702, 1082, 1525
TspGWI ACGGA 1 cut(s) 597
TspRI CASTG 2 cut(s) 441, 1477
Van91I CCANNNNNTGG 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 325
XceI RCATGY 3 cut(s) 592, 1269, 1338
XmiI GTMKAC 4 cut(s) 147, 234, 315, 735
XspI CTAG 2 cut(s) 24, 1248
Zsp2I ATGCAT 1 cut(s) 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.