AT5G35995

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005679
Physical Location & Seq
Reverse (-)
14136394 .. 14138647
2254 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35995.2

Sequence Viewer

Length: 1368 bp
ATGGGCTCTAGAGATTTCATCAGCAGTTTGCCTGATGAGGTTCTTGGCAAAAAAATCTTATCTTTACTTCCGACAAAACTCGTTGTATCCACATCTGTTCTGTCCAAGAGGTGGAGGAATCTGTTCCATTTTGTAGACAGCTTTGATTTGGAAGACTCAACACCTATCAGAAATGCTGATAGCTTCTCTGATTTCATGAAAGATACAGTGGCTCTGTTAAGCAATTGTCCCATCAAGAGACTCACTCTCAATTCCCACTATGAAAAATCTAGTGTTAACCGTTGGATTCGCAGTGCCCTCAAACGCGGCTGCTTGGAACTTAACTTGCAGTCCCAATATGATCATTATCTGGATATAGGGATTTTCTTCCGCAATAATACATTGGTTAAACTCACATTATCTAGTTATCGAACTTTTCTTCGAGGCAATGTTCCACCTGAGGGCAGAGTGTTTTTCCCTGCGCTCAAGACACTTTCCCTCGGTGCTGTTGTTGCTAAGCCAGCTCTGTATAACTGGCTCATCTCTGGATGCCCTGTGCTCGAGGAATTATTCATACTTGATGTTGGTAGTGGTGATGACCAGCCAACTTGGACGAGGTCTGTGGTTAGTGCATCCATCAAGCGACTCACGATTGTTTTCCACTTTCCTCATAACACATATCCTTATGAAGACGATGTTGAGATCAAGACACCGAATCTTGAGTTTCTTGACTATTCTGCTCTTCGCTCCGATGGTTCTGATGTTGATTATCTTGATTCGCTTGCCGAAGCTAGACTGGATCTTAGATTGTGGGAGTTAACTAACACTTTTGATTTTGGTGAGGTTACAAATCTGGTTTCTGCGATAAGAAATGTCAAGACCCTTCACCTCTCGTCCAGTTCTCTTGAGGCGTTTTACTACCGCTGTTATACCATGCCGGTCTTCGACAAACTCATTCATCTATCTATTGAGAGTGACAAGGAAAACGGTTGGCAGGCATTGCCTCGTCTGCTTTTGAAATCTCCAAATCTACAAACTTTAGCTATAAAGGGTCTTCTGCACAAAGTTACTTATAGATGTGGAAACGCATGCGCTTGCACTAGCAAGCCTAAGAAAAGGTACTTATACAAACGTTATGAAGACGACAGTCCTACTTCTGAGGTGGAAGGAAGGTGTTGTTTATCGACATGTCGAGTCAAAGTGCTAGAAATTTCAGGCTATGGAGGAAGTCTTAGAGAGGTGAAACAAATGGAGCATTTCTTGAGAAAATTGAAATATCTTGAAACTGTGAAAATTGGTGTTGAAGAAGACAACGATAGTGAGCACTTGCGCACCGAACTTATGACTCTTGATATAGCTTCATCAAAGTGCAACATCCAATTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

455

Amino Acids

51.91

Weight (kDa)

7.54

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 92 - 212 3.3e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1310
AccB7I CCANNNNNTGG 1 cut(s) 111
AccI GTMKAC 1 cut(s) 135
AccII CGCG 1 cut(s) 306
AciI CCGC 3 cut(s) 306, 370, 901
AclI AACGTT 1 cut(s) 1111
AclWI GGATC 1 cut(s) 786
AcsI RAATTY 1 cut(s) 1188
AfaI GTAC 1 cut(s) 1100
AfiI CCNNNNNNNGG 2 cut(s) 111, 440
AflIII ACRYGT 1 cut(s) 1166
AgsI TTSAA 4 cut(s) 997, 1252, 1262, 1283
AluBI AGCT 6 cut(s) 141, 183, 503, 770, 1022, 1337
AluI AGCT 6 cut(s) 141, 183, 503, 770, 1022, 1337
Alw21I GWGCWC 2 cut(s) 540, 1305
Alw26I GTCTC 1 cut(s) 232
AlwI GGATC 1 cut(s) 786
Ama87I CYCGRG 1 cut(s) 539
ApeKI GCWGC 1 cut(s) 309
ApoI RAATTY 1 cut(s) 1188
Asp700I GAANNNNTTC 1 cut(s) 122
AspLEI GCGC 3 cut(s) 463, 1073, 1311
AsuHPI GGTGA 4 cut(s) 584, 830, 857, 1231
AvaI CYCGRG 1 cut(s) 539
AxyI CCTNAGG 1 cut(s) 438
BaeGI GKGCMC 1 cut(s) 298
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 6 cut(s) 159, 675, 913, 1025, 1125, 1293
Bbv12I GWGCWC 2 cut(s) 540, 1305
BbvI GCAGC 1 cut(s) 296
BccI CCATC 3 cut(s) 239, 623, 725
BciVI GTATCC 1 cut(s) 97
BclI TGATCA 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 232
BfaI CTAG 6 cut(s) 9, 270, 402, 771, 1080, 1184
BfuI GTATCC 1 cut(s) 97
BisI GCNGC 2 cut(s) 307, 310
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 2 cut(s) 308, 311
BmeT110I CYCGRG 1 cut(s) 539
BmsI GCATC 2 cut(s) 518, 620
BoxI GACNNNNGTC 1 cut(s) 1125
BpiI GAAGAC 6 cut(s) 159, 675, 913, 1025, 1125, 1293
BplI GAGNNNNNCTC 2 cut(s) 229, 261
Bpu1102I GCTNAGC 1 cut(s) 495
BpuEI CTTGAG 4 cut(s) 449, 719, 905, 1261
BsaBI GATNNNNATC 1 cut(s) 345
BsaJI CCNNGG 1 cut(s) 478
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 440
Bse118I RCCGGY 1 cut(s) 916
Bse1I ACTGG 3 cut(s) 518, 780, 876
Bse21I CCTNAGG 1 cut(s) 438
Bse3DI GCAATG 2 cut(s) 433, 977
Bse8I GATNNNNATC 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 478
BseGI GGATG 3 cut(s) 533, 611, 1353
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 2 cut(s) 111, 440
BseMI GCAATG 2 cut(s) 433, 977
BseMII CTCAG 2 cut(s) 429, 1128
BseNI ACTGG 3 cut(s) 518, 780, 876
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 1 cut(s) 296
BsgI GTGCAG 1 cut(s) 1022
Bsh1236I CGCG 1 cut(s) 306
BsiHKAI GWGCWC 2 cut(s) 540, 1305
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 1 cut(s) 917
BslFI GGGAC 2 cut(s) 213, 316
BslI CCNNNNNNNGG 2 cut(s) 111, 440
BsmAI GTCTC 1 cut(s) 232
BsmFI GGGAC 2 cut(s) 213, 316
BsoBI CYCGRG 1 cut(s) 539
Bsp1286I GDGCHC 4 cut(s) 8, 298, 540, 1305
Bsp143I GATC 3 cut(s) 340, 681, 778
Bsp1720I GCTNAGC 1 cut(s) 495
BspACI CCGC 3 cut(s) 306, 370, 901
BspCNI CTCAG 2 cut(s) 430, 1129
BspFNI CGCG 1 cut(s) 306
BspHI TCATGA 1 cut(s) 195
BspPI GGATC 1 cut(s) 786
BspQI GCTCTTC 1 cut(s) 726
BsrDI GCAATG 2 cut(s) 433, 977
BsrFI RCCGGY 1 cut(s) 916
BsrI ACTGG 3 cut(s) 518, 780, 876
BssAI RCCGGY 1 cut(s) 916
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 3 cut(s) 340, 681, 778
Bst4CI ACNGT 5 cut(s) 208, 281, 968, 1127, 1267
Bst6I CTCTTC 1 cut(s) 726
BstAPI GCANNNNNTGC 1 cut(s) 979
BstC8I GCNNGC 6 cut(s) 501, 762, 975, 1069, 1075, 1085
BstDEI CTNAG 6 cut(s) 438, 495, 782, 1089, 1137, 1211
BstF5I GGATG 3 cut(s) 533, 611, 1353
BstFNI CGCG 1 cut(s) 306
BstHHI GCGC 3 cut(s) 463, 1073, 1311
BstKTI GATC 3 cut(s) 343, 684, 781
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 3 cut(s) 340, 681, 778
BstMWI GCNNNNNNNGC 4 cut(s) 491, 500, 979, 988
BstNSI RCATGY 2 cut(s) 1071, 1170
BstPAI GACNNNNGTC 1 cut(s) 1125
BstSLI GKGCMC 1 cut(s) 298
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 296
BstV2I GAAGAC 6 cut(s) 159, 675, 913, 1025, 1125, 1293
BstX2I RGATCY 1 cut(s) 778
BstYI RGATCY 1 cut(s) 778
Bsu36I CCTNAGG 1 cut(s) 438
BsuI GTATCC 1 cut(s) 97
BtsCI GGATG 3 cut(s) 533, 611, 1353
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 2 cut(s) 213, 298
Cac8I GCNNGC 6 cut(s) 501, 762, 975, 1069, 1075, 1085
CciI TCATGA 1 cut(s) 195
CfoI GCGC 3 cut(s) 463, 1073, 1311
Cfr10I RCCGGY 1 cut(s) 916
Csp6I GTAC 1 cut(s) 1099
CviAII CATG 4 cut(s) 196, 913, 1068, 1167
CviQI GTAC 1 cut(s) 1099
DdeI CTNAG 6 cut(s) 438, 495, 782, 1089, 1137, 1211
DpnI GATC 3 cut(s) 342, 683, 780
DpnII GATC 3 cut(s) 340, 681, 778
Eam1104I CTCTTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 726
Eco24I GRGCYC 1 cut(s) 8
Eco81I CCTNAGG 1 cut(s) 438
Eco88I CYCGRG 1 cut(s) 539
EcoT38I GRGCYC 1 cut(s) 8
FaeI CATG 4 cut(s) 199, 916, 1071, 1170
FaqI GGGAC 2 cut(s) 213, 316
FatI CATG 4 cut(s) 195, 912, 1067, 1166
FbaI TGATCA 1 cut(s) 340
FblI GTMKAC 1 cut(s) 135
Fnu4HI GCNGC 2 cut(s) 307, 310
FokI GGATG 3 cut(s) 540, 598, 1340
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 2 cut(s) 307, 310
FspBI CTAG 6 cut(s) 9, 270, 402, 771, 1080, 1184
FspI TGCGCA 1 cut(s) 1310
GlaI GCGC 3 cut(s) 462, 1072, 1310
GluI GCNGC 2 cut(s) 307, 310
HapII CCGG 1 cut(s) 917
HhaI GCGC 3 cut(s) 463, 1073, 1311
Hin1II CATG 4 cut(s) 199, 916, 1071, 1170
Hin6I GCGC 3 cut(s) 461, 1071, 1309
HinP1I GCGC 3 cut(s) 461, 1071, 1309
HincII GTYRAC 2 cut(s) 277, 798
HindII GTYRAC 2 cut(s) 277, 798
HinfI GANTC 9 cut(s) 118, 155, 240, 286, 624, 694, 755, 1173, 1324
HpaI GTTAAC 2 cut(s) 277, 798
HpaII CCGG 1 cut(s) 917
HphI GGTGA 4 cut(s) 584, 830, 857, 1231
Hpy166II GTNNAC 3 cut(s) 136, 277, 798
Hpy188I TCNGA 7 cut(s) 72, 170, 190, 730, 739, 1138, 1367
Hpy8I GTNNAC 3 cut(s) 136, 277, 798
HpyAV CCTTC 3 cut(s) 872, 1139, 1143
HpyCH4III ACNGT 5 cut(s) 208, 281, 968, 1127, 1267
HpyCH4IV ACGT 1 cut(s) 1111
HpyCH4V TGCA 5 cut(s) 328, 611, 1039, 1077, 1350
HpyF10VI GCNNNNNNNGC 4 cut(s) 491, 500, 979, 988
HpyF3I CTNAG 6 cut(s) 438, 495, 782, 1089, 1137, 1211
HpySE526I ACGT 1 cut(s) 1111
Hsp92II CATG 4 cut(s) 199, 916, 1071, 1170
HspAI GCGC 3 cut(s) 461, 1071, 1309
Ksp22I TGATCA 1 cut(s) 340
KspAI GTTAAC 2 cut(s) 277, 798
Kzo9I GATC 3 cut(s) 340, 681, 778
LguI GCTCTTC 1 cut(s) 726
LmnI GCTCC 2 cut(s) 731, 1231
Lsp1109I GCAGC 1 cut(s) 296
LweI GCATC 2 cut(s) 518, 620
MaeI CTAG 6 cut(s) 9, 270, 402, 771, 1080, 1184
MaeII ACGT 1 cut(s) 1111
MaeIII GTNAC 3 cut(s) 823, 953, 1045
MalI GATC 3 cut(s) 342, 683, 780
MboI GATC 3 cut(s) 340, 681, 778
MfeI CAATTG 1 cut(s) 223
MflI RGATCY 1 cut(s) 778
MhlI GDGCHC 4 cut(s) 8, 298, 540, 1305
MluCI AATT 7 cut(s) 223, 250, 545, 1188, 1247, 1272, 1358
MlyI GAGTC 5 cut(s) 149, 234, 618, 1182, 1318
MmeI TCCRAC 2 cut(s) 95, 263
MroXI GAANNNNTTC 1 cut(s) 122
MseI TTAA 5 cut(s) 218, 276, 321, 387, 797
MslI CAYNNNNRTG 1 cut(s) 1345
MspA1I CMGCKG 1 cut(s) 903
MspI CCGG 1 cut(s) 917
MunI CAATTG 1 cut(s) 223
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 4 cut(s) 491, 500, 979, 988
NdeII GATC 3 cut(s) 340, 681, 778
NlaIII CATG 4 cut(s) 199, 916, 1071, 1170
NmuCI GTSAC 1 cut(s) 953
NsbI TGCGCA 1 cut(s) 1310
NspI RCATGY 2 cut(s) 1071, 1170
PaeI GCATGC 1 cut(s) 1071
PaeR7I CTCGAG 1 cut(s) 539
PagI TCATGA 1 cut(s) 195
PciI ACATGT 1 cut(s) 1166
PciSI GCTCTTC 1 cut(s) 726
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 4 cut(s) 118, 286, 694, 755
PflFI GACNNNGTC 1 cut(s) 595
PflMI CCANNNNNTGG 1 cut(s) 111
PkrI GCNGC 2 cut(s) 308, 311
PleI GAGTC 5 cut(s) 149, 234, 618, 1181, 1318
PpsI GAGTC 5 cut(s) 149, 234, 618, 1181, 1318
PscI ACATGT 1 cut(s) 1166
PshAI GACNNNNGTC 1 cut(s) 1125
Psp1406I AACGTT 1 cut(s) 1111
PspXI VCTCGAGB 1 cut(s) 539
PsuI RGATCY 1 cut(s) 778
PsyI GACNNNGTC 1 cut(s) 595
RsaI GTAC 1 cut(s) 1100
RsaNI GTAC 1 cut(s) 1099
RseI CAYNNNNRTG 1 cut(s) 1345
SapI GCTCTTC 1 cut(s) 726
SaqAI TTAA 5 cut(s) 218, 276, 321, 387, 797
SatI GCNGC 2 cut(s) 307, 310
Sau3AI GATC 3 cut(s) 340, 681, 778
SchI GAGTC 5 cut(s) 149, 234, 618, 1182, 1318
SduI GDGCHC 4 cut(s) 8, 298, 540, 1305
SfaNI GCATC 2 cut(s) 518, 620
Sfr274I CTCGAG 1 cut(s) 539
SlaI CTCGAG 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 1345
SmlI CTYRAG 5 cut(s) 464, 539, 698, 884, 1240
SmoI CTYRAG 5 cut(s) 464, 539, 698, 884, 1240
SphI GCATGC 1 cut(s) 1071
Sse9I AATT 7 cut(s) 223, 250, 545, 1188, 1247, 1272, 1358
SsiI CCGC 3 cut(s) 306, 370, 901
SspMI CTAG 6 cut(s) 9, 270, 402, 771, 1080, 1184
TaaI ACNGT 5 cut(s) 208, 281, 968, 1127, 1267
TaiI ACGT 1 cut(s) 1114
TaqI TCGA 6 cut(s) 409, 421, 540, 924, 1163, 1171
TasI AATT 7 cut(s) 223, 250, 545, 1188, 1247, 1272, 1358
TauI GCSGC 1 cut(s) 309
TfiI GAWTC 4 cut(s) 118, 286, 694, 755
Tru1I TTAA 5 cut(s) 218, 276, 321, 387, 797
Tru9I TTAA 5 cut(s) 218, 276, 321, 387, 797
TscAI CASTG 2 cut(s) 213, 298
TseFI GTSAC 1 cut(s) 953
TseI GCWGC 1 cut(s) 309
Tsp45I GTSAC 1 cut(s) 953
TspRI CASTG 2 cut(s) 213, 298
Tth111I GACNNNGTC 1 cut(s) 595
Van91I CCANNNNNTGG 1 cut(s) 111
XapI RAATTY 1 cut(s) 1188
XbaI TCTAGA 1 cut(s) 8
XceI RCATGY 2 cut(s) 1071, 1170
XhoI CTCGAG 1 cut(s) 539
XmiI GTMKAC 1 cut(s) 135
XmnI GAANNNNTTC 1 cut(s) 122
XspI CTAG 6 cut(s) 9, 270, 402, 771, 1080, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.