AT4G27050

F-box LRR-repeat protein At2g29930-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005679
Physical Location & Seq
Reverse (-)
13575671 .. 13578061
2391 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27050.3

Sequence Viewer

Length: 1362 bp
ATGGACACAGATCTGATCAGCAATCTCCCCGATGATGTTCTTGGCAAAATCCTGTCTTTAGTTCCGACAAAGCTTGCTGCTGCAACGTCGGTTCTGTCCAAGAGGTGGAGGAATCTGCTTCCGCTTGTAGATAGCCTTGATTTTGACGAAACGATGCTTTTCTATCCCGACAACAAAGACGGAGAGGACGAAGCATCTAGTTACGAGAGTGGTCAACGTCGACGTCATAGCTTCTCTCATTTCGTGGACAAAACGCTTGCTCTGTTAAGCAATGCTCCTCTTACCGAGAAATTCTCTCTTAAATGCTATCACGAAGATGATGGAGCTCGTATCAACCGTTGGATTCGCACCGCGCTAGAACGTGGCTGCTTGGAGCTAAACTTAGAGGCAGCCAATTATCTACCTATAGATTCTCAGTATTTCACTAGCAACACACTGGTTAAACTCACCATTTCCGATGGATTTTATCCTGGAGGCCATCTTCTTCCTTTTGGGGGTGTGTTTTTCCCTGCGCTCAAAACACTTACTCTCGTTTCAGTTTGTTTTACAAGTGTTGAGATGATTAACTTCTTCATCCATGGATGCCCTGCGCTTGAGGAATTATTCCTATGCGTTTGGATGCAATACATGTCAAGTCCAAACGTAAAGCGAGTTACCATCACTAGTGATTTTGATGACGATATCCAACGTCCTAGCATGGTGCTGAAGACACCGAGTCTTGTGTACCTTGACTACTCCAGTTATGTCGCTGGAAACTATTATGTTGTTTTGGATTCGCTAGTCGAAGCTAGACTGGATCTTCGATTATATGAGCCAATCATCTATGATGAGGATGGTTTGTGGAATGCAGAAGTTTTTGGCAATATTACGAATCTGATTGAGGCGATCAGAACCATTAAGATCCTTCACTTGTCTCCTAGTTCTCTCGAGGTGTTTTACTATTGTTGTGATTTGCCGGTGTTGGAAGATCTCGTTAATTTATCTATCGAAAGTGACAAGGAAAAAGGCTGGGAAGTAATGCCAAGGCTGCTCAACAAGTCCCCAAATCTACAAACTTTAGTCGTCAAGGGTCTTGTGCACAGAGTTACATATTGTTGTGGAGACGCGTGCGCTTGCATCCGTATGAAGGACAGGGAGATAGTATCCTGTTTGTCGAGGTGTCGAGTGAAGGTGCTTAAGATTTTAGGGTACGGAGGAAGTTTTAGAGAGCTGAAACAAATGAGGCATTTTTTGGGGAAGTTGAAATGTCTCGAAACTGTGAAAGTTGGCGTTAAAGAGGGCAGCAAAAAGAGTAACTACTTGGTAGCTAATGTAATGGCTCTCCCCAGAGTTTCATCAAAATGCAAGATTCAATTCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_2

Protein Analysis

453

Amino Acids

51.3

Weight (kDa)

6.12

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 6 - 40 4.8e-07 F-box domain
LRR_At5g56370 PF24758 112 - 224 2.9e-07 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 226
AccB7I CCANNNNNTGG 1 cut(s) 105
AccI GTMKAC 1 cut(s) 220
AccII CGCG 2 cut(s) 353, 1106
AciI CCGC 2 cut(s) 122, 351
AclWI GGATC 2 cut(s) 804, 895
AcsI RAATTY 1 cut(s) 290
AcuI CTGAAG 1 cut(s) 725
AcyI GRCGYC 1 cut(s) 223
AfaI GTAC 2 cut(s) 725, 1190
AfiI CCNNNNNNNGG 3 cut(s) 105, 494, 1126
AflII CTTAAG 1 cut(s) 1175
AflIII ACRYGT 2 cut(s) 627, 1104
AgsI TTSAA 2 cut(s) 1243, 1352
AhdI GACNNNNNGTC 1 cut(s) 714
AhlI ACTAGT 1 cut(s) 662
AjnI CCWGG 1 cut(s) 469
AluBI AGCT 7 cut(s) 73, 231, 326, 376, 788, 1210, 1307
AluI AGCT 7 cut(s) 73, 231, 326, 376, 788, 1210, 1307
Alw21I GWGCWC 2 cut(s) 328, 1080
Alw26I GTCTC 3 cut(s) 918, 1095, 1253
Alw44I GTGCAC 1 cut(s) 1076
AlwI GGATC 2 cut(s) 804, 895
Ama87I CYCGRG 1 cut(s) 926
AoxI GGCC 1 cut(s) 475
ApaLI GTGCAC 1 cut(s) 1076
ApeKI GCWGC 6 cut(s) 77, 80, 366, 389, 1027, 1281
ApoI RAATTY 1 cut(s) 290
ArsI GACNNNNNNTTYG 2 cut(s) 783, 815
AspLEI GCGC 4 cut(s) 355, 514, 592, 1112
AsuHPI GGTGA 1 cut(s) 439
AvaI CYCGRG 1 cut(s) 926
BaeGI GKGCMC 1 cut(s) 1080
BanII GRGCYC 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 713
Bbv12I GWGCWC 2 cut(s) 328, 1080
BbvI GCAGC 6 cut(s) 64, 67, 353, 401, 1014, 1293
BccI CCATC 5 cut(s) 314, 452, 486, 665, 827
BciT130I CCWGG 1 cut(s) 471
BciVI GTATCC 1 cut(s) 1153
BclI TGATCA 1 cut(s) 15
BcoDI GTCTC 3 cut(s) 918, 1095, 1253
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 8 cut(s) 198, 356, 426, 663, 693, 779, 789, 918
BfmI CTRYAG 1 cut(s) 405
BfrI CTTAAG 1 cut(s) 1175
BfuI GTATCC 1 cut(s) 1153
BglII AGATCT 2 cut(s) 10, 967
BisI GCNGC 6 cut(s) 78, 81, 367, 390, 1028, 1282
BlsI GCNGC 6 cut(s) 79, 82, 368, 391, 1029, 1283
Bme1390I CCNGG 1 cut(s) 471
BmeRI GACNNNNNGTC 1 cut(s) 714
BmeT110I CYCGRG 1 cut(s) 926
BmrFI CCNGG 1 cut(s) 471
BmsI GCATC 5 cut(s) 144, 203, 572, 609, 1125
BpiI GAAGAC 1 cut(s) 713
BplI GAGNNNNNCTC 2 cut(s) 278, 310
BpmI CTGGAG 2 cut(s) 492, 721
BpuEI CTTGAG 1 cut(s) 614
BsaHI GRCGYC 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 577, 1022
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 494, 1126
Bse118I RCCGGY 1 cut(s) 955
Bse1I ACTGG 3 cut(s) 441, 738, 798
Bse3DI GCAATG 1 cut(s) 277
BseBI CCWGG 1 cut(s) 471
BseDI CCNNGG 2 cut(s) 577, 1022
BseGI GGATG 5 cut(s) 573, 587, 624, 838, 1116
BseLI CCNNNNNNNGG 3 cut(s) 105, 494, 1126
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 428
BseNI ACTGG 3 cut(s) 441, 738, 798
BseRI GAGGAG 1 cut(s) 267
BseSI GKGCMC 1 cut(s) 1080
BseXI GCAGC 6 cut(s) 64, 67, 353, 401, 1014, 1293
BseYI CCCAGC 1 cut(s) 1008
Bsh1236I CGCG 2 cut(s) 353, 1106
BshFI GGCC 1 cut(s) 477
BsiHKAI GWGCWC 2 cut(s) 328, 1080
BsiHKCI CYCGRG 1 cut(s) 926
BsiSI CCGG 1 cut(s) 956
BslFI GGGAC 1 cut(s) 1024
BslI CCNNNNNNNGG 3 cut(s) 105, 494, 1126
BsmAI GTCTC 3 cut(s) 918, 1095, 1253
BsmBI CGTCTC 1 cut(s) 1095
BsmFI GGGAC 1 cut(s) 1024
BsmI GAATGC 1 cut(s) 850
BsnI GGCC 1 cut(s) 477
BsoBI CYCGRG 1 cut(s) 926
Bsp1286I GDGCHC 2 cut(s) 328, 1080
Bsp143I GATC 6 cut(s) 10, 15, 796, 885, 900, 967
Bsp19I CCATGG 1 cut(s) 577
BspACI CCGC 2 cut(s) 122, 351
BspANI GGCC 1 cut(s) 477
BspCNI CTCAG 1 cut(s) 427
BspFNI CGCG 2 cut(s) 353, 1106
BspPI GGATC 2 cut(s) 804, 895
BspTI CTTAAG 1 cut(s) 1175
BsrDI GCAATG 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 955
BsrI ACTGG 3 cut(s) 441, 738, 798
BssAI RCCGGY 1 cut(s) 955
BssECI CCNNGG 2 cut(s) 577, 1022
BssMI GATC 6 cut(s) 10, 15, 796, 885, 900, 967
BssNI GRCGYC 1 cut(s) 223
BssT1I CCWWGG 2 cut(s) 577, 1022
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 2 cut(s) 338, 1258
BstACI GRCGYC 1 cut(s) 223
BstAFI CTTAAG 1 cut(s) 1175
BstC8I GCNNGC 4 cut(s) 75, 258, 1108, 1114
BstDEI CTNAG 2 cut(s) 382, 414
BstDSI CCRYGG 1 cut(s) 577
BstF5I GGATG 5 cut(s) 573, 587, 624, 838, 1116
BstFNI CGCG 2 cut(s) 353, 1106
BstHHI GCGC 4 cut(s) 355, 514, 592, 1112
BstKTI GATC 6 cut(s) 13, 18, 799, 888, 903, 970
BstMAI GTCTC 3 cut(s) 918, 1095, 1253
BstMBI GATC 6 cut(s) 10, 15, 796, 885, 900, 967
BstMWI GCNNNNNNNGC 1 cut(s) 1027
BstNI CCWGG 1 cut(s) 471
BstNSI RCATGY 1 cut(s) 631
BstSCI CCNGG 1 cut(s) 469
BstSFI CTRYAG 1 cut(s) 405
BstSLI GKGCMC 1 cut(s) 1080
BstUI CGCG 2 cut(s) 353, 1106
BstV1I GCAGC 6 cut(s) 64, 67, 353, 401, 1014, 1293
BstV2I GAAGAC 1 cut(s) 713
BstX2I RGATCY 4 cut(s) 10, 796, 900, 967
BstYI RGATCY 4 cut(s) 10, 796, 900, 967
BsuI GTATCC 1 cut(s) 1153
BsuRI GGCC 1 cut(s) 477
BtgI CCRYGG 1 cut(s) 577
BtsCI GGATG 5 cut(s) 573, 587, 624, 838, 1116
BtsIMutI CAGTG 1 cut(s) 434
Cac8I GCNNGC 4 cut(s) 75, 258, 1108, 1114
CfoI GCGC 4 cut(s) 355, 514, 592, 1112
Cfr10I RCCGGY 1 cut(s) 955
CseI GACGC 1 cut(s) 1112
Csp6I GTAC 2 cut(s) 724, 1189
CviAII CATG 3 cut(s) 578, 628, 697
CviQI GTAC 2 cut(s) 724, 1189
DdeI CTNAG 2 cut(s) 382, 414
DpnI GATC 6 cut(s) 12, 17, 798, 887, 902, 969
DpnII GATC 6 cut(s) 10, 15, 796, 885, 900, 967
DriI GACNNNNNGTC 1 cut(s) 714
Eam1105I GACNNNNNGTC 1 cut(s) 714
Ecl136II GAGCTC 1 cut(s) 326
Eco130I CCWWGG 2 cut(s) 577, 1022
Eco24I GRGCYC 1 cut(s) 328
Eco32I GATATC 1 cut(s) 682
Eco53kI GAGCTC 1 cut(s) 326
Eco57I CTGAAG 1 cut(s) 725
Eco88I CYCGRG 1 cut(s) 926
EcoICRI GAGCTC 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 469
EcoRV GATATC 1 cut(s) 682
EcoT14I CCWWGG 2 cut(s) 577, 1022
EcoT38I GRGCYC 1 cut(s) 328
ErhI CCWWGG 2 cut(s) 577, 1022
Esp3I CGTCTC 1 cut(s) 1095
FaeI CATG 3 cut(s) 581, 631, 700
FaqI GGGAC 1 cut(s) 1024
FatI CATG 3 cut(s) 577, 627, 696
FauNDI CATATG 1 cut(s) 1358
FbaI TGATCA 1 cut(s) 15
FblI GTMKAC 1 cut(s) 220
Fnu4HI GCNGC 6 cut(s) 78, 81, 367, 390, 1028, 1282
FokI GGATG 5 cut(s) 560, 594, 631, 845, 1103
FriOI GRGCYC 1 cut(s) 328
Fsp4HI GCNGC 6 cut(s) 78, 81, 367, 390, 1028, 1282
FspBI CTAG 8 cut(s) 198, 356, 426, 663, 693, 779, 789, 918
GlaI GCGC 4 cut(s) 354, 513, 591, 1111
GluI GCNGC 6 cut(s) 78, 81, 367, 390, 1028, 1282
GsaI CCCAGC 1 cut(s) 1012
GsuI CTGGAG 2 cut(s) 492, 721
HaeIII GGCC 1 cut(s) 477
HapII CCGG 1 cut(s) 956
HgaI GACGC 1 cut(s) 1112
HhaI GCGC 4 cut(s) 355, 514, 592, 1112
Hin1I GRCGYC 1 cut(s) 223
Hin1II CATG 3 cut(s) 581, 631, 700
Hin6I GCGC 4 cut(s) 353, 512, 590, 1110
HinP1I GCGC 4 cut(s) 353, 512, 590, 1110
HincII GTYRAC 2 cut(s) 215, 221
HindII GTYRAC 2 cut(s) 215, 221
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 7 cut(s) 112, 343, 410, 715, 773, 871, 1348
HpaII CCGG 1 cut(s) 956
HphI GGTGA 1 cut(s) 439
Hpy166II GTNNAC 5 cut(s) 215, 221, 247, 724, 1078
Hpy188I TCNGA 5 cut(s) 15, 66, 457, 876, 890
Hpy188III TCNNGA 4 cut(s) 167, 311, 926, 1250
Hpy8I GTNNAC 5 cut(s) 215, 221, 247, 724, 1078
Hpy99I CGWCG 3 cut(s) 91, 222, 225
HpyAV CCTTC 3 cut(s) 914, 1120, 1162
HpyCH4III ACNGT 2 cut(s) 338, 1258
HpyCH4IV ACGT 6 cut(s) 86, 217, 223, 361, 642, 688
HpyCH4V TGCA 6 cut(s) 83, 622, 848, 1078, 1116, 1344
HpyF10VI GCNNNNNNNGC 1 cut(s) 1027
HpyF3I CTNAG 2 cut(s) 382, 414
HpySE526I ACGT 6 cut(s) 86, 217, 223, 361, 642, 688
Hsp92I GRCGYC 1 cut(s) 223
Hsp92II CATG 3 cut(s) 581, 631, 700
HspAI GCGC 4 cut(s) 353, 512, 590, 1110
Ksp22I TGATCA 1 cut(s) 15
Kzo9I GATC 6 cut(s) 10, 15, 796, 885, 900, 967
LmnI GCTCC 3 cut(s) 280, 323, 373
Lsp1109I GCAGC 6 cut(s) 64, 67, 353, 401, 1014, 1293
LweI GCATC 5 cut(s) 144, 203, 572, 609, 1125
MaeI CTAG 8 cut(s) 198, 356, 426, 663, 693, 779, 789, 918
MaeII ACGT 6 cut(s) 86, 217, 223, 361, 642, 688
MaeIII GTNAC 5 cut(s) 200, 652, 992, 1084, 1292
MalI GATC 6 cut(s) 12, 17, 798, 887, 902, 969
MboI GATC 6 cut(s) 10, 15, 796, 885, 900, 967
MboII GAAGA 7 cut(s) 326, 473, 476, 562, 718, 791, 977
MflI RGATCY 4 cut(s) 10, 796, 900, 967
MhlI GDGCHC 2 cut(s) 328, 1080
MluCI AATT 5 cut(s) 290, 394, 599, 976, 1352
MluI ACGCGT 1 cut(s) 1104
MlyI GAGTC 1 cut(s) 724
MmeI TCCRAC 4 cut(s) 89, 320, 709, 942
MseI TTAA 8 cut(s) 266, 300, 441, 564, 897, 975, 1176, 1272
MslI CAYNNNNRTG 3 cut(s) 315, 1121, 1339
MspCI CTTAAG 1 cut(s) 1175
MspI CCGG 1 cut(s) 956
MspR9I CCNGG 1 cut(s) 471
Mva1269I GAATGC 1 cut(s) 850
MvaI CCWGG 1 cut(s) 471
MvnI CGCG 2 cut(s) 353, 1106
MwoI GCNNNNNNNGC 1 cut(s) 1027
NcoI CCATGG 1 cut(s) 577
NdeI CATATG 1 cut(s) 1358
NdeII GATC 6 cut(s) 10, 15, 796, 885, 900, 967
NlaIII CATG 3 cut(s) 581, 631, 700
NmuCI GTSAC 1 cut(s) 992
NspI RCATGY 1 cut(s) 631
PaeR7I CTCGAG 1 cut(s) 926
PciI ACATGT 1 cut(s) 627
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 334
PctI GAATGC 1 cut(s) 850
PfeI GAWTC 6 cut(s) 112, 343, 410, 773, 871, 1348
PflMI CCANNNNNTGG 1 cut(s) 105
PfoI TCCNGGA 1 cut(s) 469
PkrI GCNGC 6 cut(s) 79, 82, 368, 391, 1029, 1283
PleI GAGTC 1 cut(s) 723
PpsI GAGTC 1 cut(s) 723
PscI ACATGT 1 cut(s) 627
Psp124BI GAGCTC 1 cut(s) 328
Psp6I CCWGG 1 cut(s) 469
PspFI CCCAGC 1 cut(s) 1008
PspGI CCWGG 1 cut(s) 469
PsuI RGATCY 4 cut(s) 10, 796, 900, 967
RsaI GTAC 2 cut(s) 725, 1190
RsaNI GTAC 2 cut(s) 724, 1189
RseI CAYNNNNRTG 3 cut(s) 315, 1121, 1339
SacI GAGCTC 1 cut(s) 328
SalI GTCGAC 1 cut(s) 219
SaqAI TTAA 8 cut(s) 266, 300, 441, 564, 897, 975, 1176, 1272
SatI GCNGC 6 cut(s) 78, 81, 367, 390, 1028, 1282
Sau3AI GATC 6 cut(s) 10, 15, 796, 885, 900, 967
SchI GAGTC 1 cut(s) 724
ScrFI CCNGG 1 cut(s) 471
SduI GDGCHC 2 cut(s) 328, 1080
SfaNI GCATC 5 cut(s) 144, 203, 572, 609, 1125
SfcI CTRYAG 1 cut(s) 405
Sfr274I CTCGAG 1 cut(s) 926
SgrDI CGTCGACG 1 cut(s) 219
SlaI CTCGAG 1 cut(s) 926
SmiMI CAYNNNNRTG 3 cut(s) 315, 1121, 1339
SmlI CTYRAG 3 cut(s) 593, 926, 1175
SmoI CTYRAG 3 cut(s) 593, 926, 1175
SpeI ACTAGT 1 cut(s) 662
Sse9I AATT 5 cut(s) 290, 394, 599, 976, 1352
SsiI CCGC 2 cut(s) 122, 351
SspI AATATT 1 cut(s) 865
SspMI CTAG 8 cut(s) 198, 356, 426, 663, 693, 779, 789, 918
SstI GAGCTC 1 cut(s) 328
StyD4I CCNGG 1 cut(s) 469
StyI CCWWGG 2 cut(s) 577, 1022
TaaI ACNGT 2 cut(s) 338, 1258
TaiI ACGT 6 cut(s) 89, 220, 226, 364, 645, 691
TaqI TCGA 8 cut(s) 220, 783, 802, 927, 987, 1154, 1162, 1251
TasI AATT 5 cut(s) 290, 394, 599, 976, 1352
TfiI GAWTC 6 cut(s) 112, 343, 410, 773, 871, 1348
Tru1I TTAA 8 cut(s) 266, 300, 441, 564, 897, 975, 1176, 1272
Tru9I TTAA 8 cut(s) 266, 300, 441, 564, 897, 975, 1176, 1272
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 1 cut(s) 992
TseI GCWGC 6 cut(s) 77, 80, 366, 389, 1027, 1281
Tsp45I GTSAC 1 cut(s) 992
TspDTI ATGAA 4 cut(s) 562, 1139, 1323, 1345
TspGWI ACGGA 3 cut(s) 195, 1109, 1206
TspRI CASTG 1 cut(s) 441
Van91I CCANNNNNTGG 1 cut(s) 105
Vha464I CTTAAG 1 cut(s) 1175
VneI GTGCAC 1 cut(s) 1076
XapI RAATTY 1 cut(s) 290
XceI RCATGY 1 cut(s) 631
XhoI CTCGAG 1 cut(s) 926
XmiI GTMKAC 1 cut(s) 220
XspI CTAG 8 cut(s) 198, 356, 426, 663, 693, 779, 789, 918
ZraI GACGTC 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.