AT1G50730

Vacuolar protein sorting-associated protein 35

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007890
Physical Location & Seq
Forward (+)
18795210 .. 18802370
7161 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G50730.3

Sequence Viewer

Length: 2772 bp
ATGGAGTTGGAATTCCGGCGTCGCGACTATGGAGCTACACATAAATCTCAATTTCTTCCTCGGTCACAAACTCAAAAGCATCCTCTTTCCTCTACACTTGCTTCTCGTGACCAACAGGCTAAAACTGTCAGAGGTCGCGATCTTCACTTCTTTGATCCATTGAGGGGCTTGGATGTGAATGCTTCTGCTGAAGAGAATGTTGAAGATACTTGTATATCCATTGAAGCTGTAACTCAGGATCTGATAAAGGAGTGGAAATCACTTAAAAGAATCTTAATGCAGCGGTTTCCTGTCTCCAAACTTCTGCTTTGCTTTACTCAATCAGCAGTTGAGTCCCCTTCTGCTCTTTCCCATCCGGAGGAAACTGGCAGCGAACAAACTTCTCTGGAAGAGCCGGCCAAAATAATCAGCCAACATGAATATATAGCCAAAGTACATGAGCTAAGAGATGGAATAAATAGCGCTTGGCAGGCTGAAGATCGAGTAACATCGTTGAAGTTATCAATAAAGGTCACAAAGCTTCTGATGGATACAACAGTTTTGCGGTTTTATCCTACAGTCTTTGTCATTGTCACTGACATGCTAGATATGCTTGGGGATATGGTGTGGGAGCGGATCAAACAGAAAGCAGAACTTGATATAGATGGAACAGTGATCTGCGCCTTACCGATGATATCTGCCTTGAAGCAAGAGAGACTTGCTATAACTGGTTTTGCAAAGTTGGTTCTGTTCGAGAACTTCTCCCTCGCATGTAAAGCCCCATCAATTATTATTTTTCTTATTCTCCCTACCTTCCTCTGCCTTAGCATTGGCCTTTCTTTGTATGCCCATAGTTATCTGGAGCTTGCGATCTTGCCTTGCTGGCGTTTCCTCATTAATCAACCCATGGAAGTTCTGGATCGTTTAGTCATGATGGTGAGAGGTCTCGCAGATCCACTGACATCTTTGTATTGTCGTCTTTATATGGTGCATCGCATGCAGAAGTTTGGGTTCTGCAGTTCAGGATATTTAATCAAATGCATCAAGGATATTGAAGATGTATTGGCGCCTGTTTTAGTGGACAAGGAGGGGTATTCCTATATTACAGACGACAAAAAGTTACTTTTTAGTTTGGTGGAACCAGCCATCGAGTATATAATGAAATGCTTATTTCTGACTGGACGTCAGGAGAACAATGTCTTGGGCATACTTGAGGAGCTTGGATTTGGACGGAACAAATTTCAGTCATCTTACAACTCTTCGCATGTGTCCATTTTACTTCATTATTTACTCAAGGAACTTCCGTCTGAATTAGTTAGTTCACTAGCAATGGAGATCTTGGATATGATTAGATGCAGCAATGATTGTTCCTTTAGTCAGGTTTTGAATTATAGGTTACTTGGAAATAGATTGTCTGAAGGGAAATCTCAAGAAGGTTTTCTCAGTTCACTCATTGATGAAGTTATTCAGGCTGCTTCTCAGTATCAGTCACTTTATGACTACTTAAGAATTATGGACGCATATGTGGATCTTATGTTGCAGAACAAGATGGAAAATCATCTGGATGCTCTCTTGGATGATATTGTAAGCCTAGCTCGCGACAAGTTTCTTTCTGAAGAAGAACAAGCAAGTTTGCAATCCATAATTTTGAAGCTGCTCTCTCATTTCGAGAACTTGCAAGAAGTACTTCCTCTGAATCATTTCATCGAGATTCTGGATCTTATGTCTGGGACTTCAAAGAGCAGTGTAAACATGCATCTTCTTAACATGGGTACAAGGAATGGCTGCATTTGTGATTCAACAACAGTGCAATTGCTTTTTGAAGTTTCTCAGGCTCTTTATGATGCTACAGATTTCGTTAACATAAAGGATGATGACAATAGACAGACATCACATTTAATTTCTCGTTTTGTTGAGATGGTTGACTATGGGGCAGAAATGGAACGCCATTTATTGTTTTTGGCGGAGTGTCGTGAGGCCTTTAATGGGATACATGAACTTAAGGAGACACTTGTTCGTTCAAGCAACACCTTGGCAGTAAAAGCACTGAAAGCAGGAAAGAAGCATATCAACTTTGTTAAATCTTGCCTAGCATTTAGCGAAGTGACCATCCCATCTATTTCATCTCCCACGAAACACTTAAATCTTTATCTTGAAACTGCTGAGGTTGCACTTTTAGGTGGTTTGATTTCTCATTCAGATGAACTGGTCATGTCAGCTGTTGAGTATCTAGAGAATGTAGTGCTGACAGATGGTTTAAAATCAATTGATATAGATAGTATGGCCTCGGTTATATGCAAATTGTGCAGCCTCTTGGTAATGATCCCAGGTAATCCCGAGAAAGGCGTGATGGAGATCCTTAAAAGTATCTTCTCTGCTACTCGCTCTAGTTCATGGGCAACACTGAGAGTAAAGGTGAAGATTTTCTGTGCTATAATGTCACTGTTGTCGACACTTTCTCAGGACAATCTGCCTTACCATTCTGCCAATCCAGAGATAATTGGAAATGAATTGCTGTTCTTTGGTGATTCATCATACAAGCAGGAACTTGTCTCGTGTACTCAGTTAGTTCTAAGTGAACTATTAGATGCTATTGAGCAAGAGTCTTCACAGATTTCTCGGGGGAACATGGCGCTTGAAGCTTGCAATTGCATATCATCAGCACTAGTGATGAATGAGAAAGTCAAAGAACTTTGCTTGAGACTGCTGGAAACAGCAAAAGGTTGTTTGGGTGCCAATGACAGGTACATCGAATCGACTAAGAAATCACTCCAGCTAAGATACACCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps35

Protein Analysis

923

Amino Acids

103.97

Weight (kDa)

5.33

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps35 PF03635 460 - 746 2.4e-10 Vacuolar protein sorting-associated protein 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1165
AccB1I GGYRCC 2 cut(s) 1045, 2711
AccBSI CCGCTC 1 cut(s) 613
AccI GTMKAC 1 cut(s) 2429
AccII CGCG 3 cut(s) 24, 138, 1578
AccIII TCCGGA 1 cut(s) 355
AciI CCGC 4 cut(s) 283, 544, 613, 1943
AclWI GGATC 9 cut(s) 149, 246, 623, 906, 926, 1515, 1704, 2296, 2329
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 2 cut(s) 11, 1217
AcuI CTGAAG 4 cut(s) 210, 495, 1416, 1614
AcyI GRCGYC 3 cut(s) 19, 1046, 1162
AdeI CACNNNGTG 1 cut(s) 2769
AfaI GTAC 5 cut(s) 435, 1665, 1753, 2539, 2726
AfeI AGCGCT 1 cut(s) 463
AfiI CCNNNNNNNGG 5 cut(s) 164, 358, 1965, 2321, 2721
AflII CTTAAG 2 cut(s) 1483, 1979
AhdI GACNNNNNGTC 1 cut(s) 1161
AhlI ACTAGT 1 cut(s) 2644
AjnI CCWGG 1 cut(s) 2305
AjuI GAANNNNNNNTTGG 2 cut(s) 1163, 1195
Alw26I GTCTC 6 cut(s) 298, 688, 929, 1979, 2536, 2674
AlwI GGATC 9 cut(s) 149, 246, 623, 906, 926, 1515, 1704, 2296, 2329
AlwNI CAGNNNCTG 1 cut(s) 241
Ama87I CYCGRG 2 cut(s) 2315, 2598
Aor13HI TCCGGA 1 cut(s) 355
Aor51HI AGCGCT 1 cut(s) 463
AoxI GGCC 4 cut(s) 396, 811, 1956, 2262
ApeKI GCWGC 7 cut(s) 280, 369, 1335, 1451, 1633, 1764, 2286
ApoI RAATTY 2 cut(s) 11, 1217
AseI ATTAAT 1 cut(s) 876
Asp700I GAANNNNTTC 5 cut(s) 1416, 1443, 1665, 1679, 2402
AspLEI GCGC 4 cut(s) 464, 662, 1048, 2614
AsuHPI GGTGA 3 cut(s) 928, 2407, 2516
AvaI CYCGRG 2 cut(s) 2315, 2598
BaeI ACNNNNGTAYC 2 cut(s) 522, 555
BanI GGYRCC 2 cut(s) 1045, 2711
BauI CACGAG 2 cut(s) 105, 2533
BbsI GAAGAC 1 cut(s) 2577
BbvCI CCTCAGC 1 cut(s) 2142
BbvI GCAGC 7 cut(s) 292, 381, 1347, 1438, 1620, 1751, 2298
BciT130I CCWGG 1 cut(s) 2307
BciVI GTATCC 2 cut(s) 523, 1962
BcoDI GTCTC 6 cut(s) 298, 688, 929, 1979, 2536, 2674
BcuI ACTAGT 1 cut(s) 2644
BfaI CTAG 7 cut(s) 584, 1304, 1571, 2069, 2210, 2367, 2645
BfmI CTRYAG 3 cut(s) 555, 994, 1827
BfoI RGCGCY 3 cut(s) 465, 1049, 2615
BfrI CTTAAG 2 cut(s) 1483, 1979
BfuI GTATCC 2 cut(s) 523, 1962
BglI GCCNNNNNGGC 1 cut(s) 862
BglII AGATCT 1 cut(s) 1314
BisI GCNGC 7 cut(s) 281, 370, 1336, 1452, 1634, 1765, 2287
BlsI GCNGC 7 cut(s) 282, 371, 1337, 1453, 1635, 1766, 2288
BmcAI AGTACT 1 cut(s) 1665
Bme1390I CCNGG 1 cut(s) 2307
BmeRI GACNNNNNGTC 1 cut(s) 1161
BmeT110I CYCGRG 2 cut(s) 2315, 2598
BmiI GGNNCC 3 cut(s) 1047, 1119, 2713
BmrFI CCNGG 1 cut(s) 2307
BmsI GCATC 8 cut(s) 88, 979, 1029, 1322, 1534, 1744, 1813, 2557
BpiI GAAGAC 1 cut(s) 2577
BplI GAGNNNNNCTC 2 cut(s) 725, 757
BpmI CTGGAG 2 cut(s) 860, 2735
Bpu10I CCTNAGC 2 cut(s) 803, 2142
BpuEI CTTGAG 4 cut(s) 1211, 1256, 1392, 2698
BsaBI GATNNNNATC 1 cut(s) 2333
BsaHI GRCGYC 3 cut(s) 19, 1046, 1162
BsaI GGTCTC 1 cut(s) 929
BsaJI CCNNGG 5 cut(s) 59, 885, 2010, 2265, 2305
BsaWI WCCGGW 1 cut(s) 355
Bsc4I CCNNNNNNNGG 5 cut(s) 164, 358, 1965, 2321, 2721
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 4 cut(s) 370, 712, 1162, 2190
Bse3DI GCAATG 2 cut(s) 1314, 1345
Bse8I GATNNNNATC 1 cut(s) 2333
BseAI TCCGGA 1 cut(s) 355
BseBI CCWGG 1 cut(s) 2307
BseDI CCNNGG 5 cut(s) 59, 885, 2010, 2265, 2305
BseGI GGATG 7 cut(s) 79, 178, 352, 1549, 1561, 1855, 2088
BseJI GATNNNNATC 1 cut(s) 2333
BseLI CCNNNNNNNGG 5 cut(s) 164, 358, 1965, 2321, 2721
BseMI GCAATG 2 cut(s) 1314, 1345
BseMII CTCAG 8 cut(s) 248, 1435, 1472, 1823, 2133, 2375, 2453, 2555
BseNI ACTGG 4 cut(s) 370, 712, 1162, 2190
BseRI GAGGAG 1 cut(s) 1208
BseXI GCAGC 7 cut(s) 292, 381, 1347, 1438, 1620, 1751, 2298
BsgI GTGCAG 1 cut(s) 2305
Bsh1236I CGCG 3 cut(s) 24, 138, 1578
BshFI GGCC 4 cut(s) 398, 813, 1958, 2264
BshNI GGYRCC 2 cut(s) 1045, 2711
BsiHKCI CYCGRG 2 cut(s) 2315, 2598
BsiSI CCGG 3 cut(s) 16, 356, 395
BslFI GGGAC 2 cut(s) 319, 1723
BslI CCNNNNNNNGG 5 cut(s) 164, 358, 1965, 2321, 2721
BsmAI GTCTC 6 cut(s) 298, 688, 929, 1979, 2536, 2674
BsmFI GGGAC 2 cut(s) 319, 1723
BsmI GAATGC 1 cut(s) 184
BsnI GGCC 4 cut(s) 398, 813, 1958, 2264
Bso31I GGTCTC 1 cut(s) 929
BsoBI CYCGRG 2 cut(s) 2315, 2598
Bsp13I TCCGGA 1 cut(s) 355
Bsp19I CCATGG 1 cut(s) 885
Bsp68I TCGCGA 3 cut(s) 24, 138, 1578
BspACI CCGC 4 cut(s) 283, 544, 613, 1943
BspANI GGCC 4 cut(s) 398, 813, 1958, 2264
BspCNI CTCAG 8 cut(s) 247, 1434, 1471, 1822, 2134, 2376, 2452, 2554
BspEI TCCGGA 1 cut(s) 355
BspFNI CGCG 3 cut(s) 24, 138, 1578
BspHI TCATGA 1 cut(s) 909
BspLI GGNNCC 3 cut(s) 1047, 1119, 2713
BspMAI CTGCAG 1 cut(s) 998
BspPI GGATC 9 cut(s) 149, 246, 623, 906, 926, 1515, 1704, 2296, 2329
BspQI GCTCTTC 1 cut(s) 384
BspT107I GGYRCC 2 cut(s) 1045, 2711
BspTI CTTAAG 2 cut(s) 1483, 1979
BspTNI GGTCTC 1 cut(s) 929
BsrBI CCGCTC 1 cut(s) 613
BsrDI GCAATG 2 cut(s) 1314, 1345
BsrFI RCCGGY 1 cut(s) 394
BsrI ACTGG 4 cut(s) 370, 712, 1162, 2190
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 5 cut(s) 59, 885, 2010, 2265, 2305
BssNI GRCGYC 3 cut(s) 19, 1046, 1162
BssSI CACGAG 2 cut(s) 105, 2533
BssT1I CCWWGG 2 cut(s) 885, 2010
Bst2BI CACGAG 2 cut(s) 105, 2533
Bst2UI CCWGG 1 cut(s) 2307
Bst4CI ACNGT 6 cut(s) 127, 538, 559, 652, 1786, 2424
Bst6I CTCTTC 3 cut(s) 186, 384, 1243
BstACI GRCGYC 3 cut(s) 19, 1046, 1162
BstAFI CTTAAG 2 cut(s) 1483, 1979
BstAPI GCANNNNNTGC 2 cut(s) 976, 2283
BstC8I GCNNGC 7 cut(s) 396, 471, 846, 863, 977, 1576, 2623
BstDSI CCRYGG 1 cut(s) 885
BstF5I GGATG 7 cut(s) 79, 178, 352, 1549, 1561, 1855, 2088
BstFNI CGCG 3 cut(s) 24, 138, 1578
BstH2I RGCGCY 3 cut(s) 465, 1049, 2615
BstHHI GCGC 4 cut(s) 464, 662, 1048, 2614
BstMAI GTCTC 6 cut(s) 298, 688, 929, 1979, 2536, 2674
BstNI CCWGG 1 cut(s) 2307
BstNSI RCATGY 5 cut(s) 583, 753, 979, 1247, 1735
BstSCI CCNGG 1 cut(s) 2305
BstSFI CTRYAG 3 cut(s) 555, 994, 1827
BstUI CGCG 3 cut(s) 24, 138, 1578
BstV1I GCAGC 7 cut(s) 292, 381, 1347, 1438, 1620, 1751, 2298
BstV2I GAAGAC 1 cut(s) 2577
BstX2I RGATCY 6 cut(s) 238, 931, 1314, 1507, 1696, 2334
BstYI RGATCY 6 cut(s) 238, 931, 1314, 1507, 1696, 2334
BsuI GTATCC 2 cut(s) 523, 1962
BsuRI GGCC 4 cut(s) 398, 813, 1958, 2264
BtgI CCRYGG 1 cut(s) 885
BtgZI GCGATG 1 cut(s) 956
BtsCI GGATG 7 cut(s) 79, 178, 352, 1549, 1561, 1855, 2088
BtsI GCAGTG 1 cut(s) 1729
BtsIMutI CAGTG 8 cut(s) 573, 657, 935, 1729, 1791, 2024, 2381, 2420
BtuMI TCGCGA 3 cut(s) 24, 138, 1578
Cac8I GCNNGC 7 cut(s) 396, 471, 846, 863, 977, 1576, 2623
CaiI CAGNNNCTG 1 cut(s) 241
CciI TCATGA 1 cut(s) 909
CfoI GCGC 4 cut(s) 464, 662, 1048, 2614
Cfr10I RCCGGY 1 cut(s) 394
CseI GACGC 2 cut(s) 8, 1505
Csp6I GTAC 5 cut(s) 434, 1664, 1752, 2538, 2725
CviQI GTAC 5 cut(s) 434, 1664, 1752, 2538, 2725
DinI GGCGCC 1 cut(s) 1047
DraI TTTAAA 1 cut(s) 2238
DraIII CACNNNGTG 1 cut(s) 2769
DriI GACNNNNNGTC 1 cut(s) 1161
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 3 cut(s) 186, 384, 1243
Eam1105I GACNNNNNGTC 1 cut(s) 1161
EarI CTCTTC 3 cut(s) 186, 384, 1243
EciI GGCGGA 1 cut(s) 1958
Eco130I CCWWGG 2 cut(s) 885, 2010
Eco147I AGGCCT 1 cut(s) 1958
Eco31I GGTCTC 1 cut(s) 929
Eco32I GATATC 1 cut(s) 675
Eco47III AGCGCT 1 cut(s) 463
Eco57I CTGAAG 4 cut(s) 210, 495, 1416, 1614
Eco88I CYCGRG 2 cut(s) 2315, 2598
EcoRI GAATTC 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 2305
EcoRV GATATC 1 cut(s) 675
EcoT14I CCWWGG 2 cut(s) 885, 2010
EcoT22I ATGCAT 2 cut(s) 1022, 1737
EgeI GGCGCC 1 cut(s) 1047
EheI GGCGCC 1 cut(s) 1047
ErhI CCWWGG 2 cut(s) 885, 2010
FalI AAGNNNNNCTT 6 cut(s) 618, 650, 681, 713, 1650, 1682
FaqI GGGAC 2 cut(s) 319, 1723
FauNDI CATATG 1 cut(s) 1501
FblI GTMKAC 1 cut(s) 2429
Fnu4HI GCNGC 7 cut(s) 281, 370, 1336, 1452, 1634, 1765, 2287
FokI GGATG 7 cut(s) 66, 185, 339, 1556, 1568, 1862, 2075
Fsp4HI GCNGC 7 cut(s) 281, 370, 1336, 1452, 1634, 1765, 2287
FspBI CTAG 7 cut(s) 584, 1304, 1571, 2069, 2210, 2367, 2645
GlaI GCGC 4 cut(s) 463, 661, 1047, 2613
GluI GCNGC 7 cut(s) 281, 370, 1336, 1452, 1634, 1765, 2287
GsuI CTGGAG 2 cut(s) 860, 2735
HaeII RGCGCY 3 cut(s) 465, 1049, 2615
HaeIII GGCC 4 cut(s) 398, 813, 1958, 2264
HapII CCGG 3 cut(s) 16, 356, 395
HgaI GACGC 2 cut(s) 8, 1505
HhaI GCGC 4 cut(s) 464, 662, 1048, 2614
Hin1I GRCGYC 3 cut(s) 19, 1046, 1162
Hin6I GCGC 4 cut(s) 462, 660, 1046, 2612
HinP1I GCGC 4 cut(s) 462, 660, 1046, 2612
HincII GTYRAC 3 cut(s) 1840, 1903, 2430
HindII GTYRAC 3 cut(s) 1840, 1903, 2430
HindIII AAGCTT 2 cut(s) 518, 2619
HinfI GANTC 8 cut(s) 270, 332, 1675, 1690, 1775, 2507, 2582, 2732
HpaI GTTAAC 1 cut(s) 1840
HpaII CCGG 3 cut(s) 16, 356, 395
HphI GGTGA 3 cut(s) 928, 2407, 2516
Hpy166II GTNNAC 9 cut(s) 1060, 1301, 1427, 1729, 1840, 1903, 2430, 2538, 2558
Hpy188I TCNGA 9 cut(s) 131, 243, 525, 1155, 1288, 1396, 1594, 1674, 2179
Hpy8I GTNNAC 9 cut(s) 1060, 1301, 1427, 1729, 1840, 1903, 2430, 2538, 2558
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 4 cut(s) 348, 802, 1391, 1406
HpyCH4III ACNGT 6 cut(s) 127, 538, 559, 652, 1786, 2424
HpyCH4IV ACGT 1 cut(s) 1162
HpySE526I ACGT 1 cut(s) 1162
Hsp92I GRCGYC 3 cut(s) 19, 1046, 1162
HspAI GCGC 4 cut(s) 462, 660, 1046, 2612
KasI GGCGCC 1 cut(s) 1045
Kpn2I TCCGGA 1 cut(s) 355
KroI GCCGGC 1 cut(s) 394
KroNI GCCGGC 1 cut(s) 396
KspAI GTTAAC 1 cut(s) 1840
LguI GCTCTTC 1 cut(s) 384
LmnI GCTCC 4 cut(s) 32, 610, 841, 1195
Lsp1109I GCAGC 7 cut(s) 292, 381, 1347, 1438, 1620, 1751, 2298
LweI GCATC 8 cut(s) 88, 979, 1029, 1322, 1534, 1744, 1813, 2557
MaeI CTAG 7 cut(s) 584, 1304, 1571, 2069, 2210, 2367, 2645
MaeII ACGT 1 cut(s) 1162
MbiI CCGCTC 1 cut(s) 613
MfeI CAATTG 3 cut(s) 1790, 2244, 2626
MflI RGATCY 6 cut(s) 238, 931, 1314, 1507, 1696, 2334
Mly113I GGCGCC 1 cut(s) 1046
MlyI GAGTC 2 cut(s) 341, 2591
Mph1103I ATGCAT 2 cut(s) 1022, 1737
MroI TCCGGA 1 cut(s) 355
MroNI GCCGGC 1 cut(s) 394
MroXI GAANNNNTTC 5 cut(s) 1416, 1443, 1665, 1679, 2402
MslI CAYNNNNRTG 4 cut(s) 578, 914, 1542, 2178
MspA1I CMGCKG 2 cut(s) 283, 2198
MspCI CTTAAG 2 cut(s) 1483, 1979
MspI CCGG 3 cut(s) 16, 356, 395
MspR9I CCNGG 1 cut(s) 2307
MunI CAATTG 3 cut(s) 1790, 2244, 2626
Mva1269I GAATGC 1 cut(s) 184
MvaI CCWGG 1 cut(s) 2307
MvnI CGCG 3 cut(s) 24, 138, 1578
NaeI GCCGGC 1 cut(s) 396
NarI GGCGCC 1 cut(s) 1046
NcoI CCATGG 1 cut(s) 885
NdeI CATATG 1 cut(s) 1501
NgoMIV GCCGGC 1 cut(s) 394
NlaIV GGNNCC 3 cut(s) 1047, 1119, 2713
NmuCI GTSAC 7 cut(s) 63, 107, 511, 571, 1467, 2083, 2418
NruI TCGCGA 3 cut(s) 24, 138, 1578
NsiI ATGCAT 2 cut(s) 1022, 1737
NspI RCATGY 5 cut(s) 583, 753, 979, 1247, 1735
PaeI GCATGC 1 cut(s) 979
PagI TCATGA 1 cut(s) 909
PceI AGGCCT 1 cut(s) 1958
PciSI GCTCTTC 1 cut(s) 384
PctI GAATGC 1 cut(s) 184
PdiI GCCGGC 1 cut(s) 396
PdmI GAANNNNTTC 5 cut(s) 1416, 1443, 1665, 1679, 2402
PfeI GAWTC 6 cut(s) 270, 1675, 1690, 1775, 2507, 2732
PkrI GCNGC 7 cut(s) 282, 371, 1337, 1453, 1635, 1766, 2288
PleI GAGTC 2 cut(s) 340, 2590
PluTI GGCGCC 1 cut(s) 1049
PpsI GAGTC 2 cut(s) 340, 2590
PshBI ATTAAT 1 cut(s) 876
Psp6I CCWGG 1 cut(s) 2305
PspGI CCWGG 1 cut(s) 2305
PspN4I GGNNCC 3 cut(s) 1047, 1119, 2713
PstI CTGCAG 1 cut(s) 998
PstNI CAGNNNCTG 1 cut(s) 241
PsuI RGATCY 6 cut(s) 238, 931, 1314, 1507, 1696, 2334
PvuII CAGCTG 1 cut(s) 2198
RruI TCGCGA 3 cut(s) 24, 138, 1578
RsaI GTAC 5 cut(s) 435, 1665, 1753, 2539, 2726
RsaNI GTAC 5 cut(s) 434, 1664, 1752, 2538, 2725
RseI CAYNNNNRTG 4 cut(s) 578, 914, 1542, 2178
SalI GTCGAC 1 cut(s) 2428
SapI GCTCTTC 1 cut(s) 384
SatI GCNGC 7 cut(s) 281, 370, 1336, 1452, 1634, 1765, 2287
ScaI AGTACT 1 cut(s) 1665
SchI GAGTC 2 cut(s) 341, 2591
ScrFI CCNGG 1 cut(s) 2307
SfaNI GCATC 8 cut(s) 88, 979, 1029, 1322, 1534, 1744, 1813, 2557
SfcI CTRYAG 3 cut(s) 555, 994, 1827
SfoI GGCGCC 1 cut(s) 1047
SmiMI CAYNNNNRTG 4 cut(s) 578, 914, 1542, 2178
SmlI CTYRAG 6 cut(s) 1190, 1271, 1407, 1483, 1979, 2677
SmoI CTYRAG 6 cut(s) 1190, 1271, 1407, 1483, 1979, 2677
SpeI ACTAGT 1 cut(s) 2644
SphI GCATGC 1 cut(s) 979
SseBI AGGCCT 1 cut(s) 1958
SsiI CCGC 4 cut(s) 283, 544, 613, 1943
SspDI GGCGCC 1 cut(s) 1045
SspMI CTAG 7 cut(s) 584, 1304, 1571, 2069, 2210, 2367, 2645
StuI AGGCCT 1 cut(s) 1958
StyD4I CCNGG 1 cut(s) 2305
StyI CCWWGG 2 cut(s) 885, 2010
TaaI ACNGT 6 cut(s) 127, 538, 559, 652, 1786, 2424
TaiI ACGT 1 cut(s) 1165
TaqI TCGA 8 cut(s) 481, 732, 1128, 1647, 1686, 2429, 2730, 2735
TaqII GACCGA 1 cut(s) 51
TatI WGTACW 3 cut(s) 433, 1663, 2537
TfiI GAWTC 6 cut(s) 270, 1675, 1690, 1775, 2507, 2732
TscAI CASTG 8 cut(s) 580, 657, 942, 1729, 1791, 2031, 2388, 2427
TseFI GTSAC 7 cut(s) 63, 107, 511, 571, 1467, 2083, 2418
TseI GCWGC 7 cut(s) 280, 369, 1335, 1451, 1633, 1764, 2286
Tsp45I GTSAC 7 cut(s) 63, 107, 511, 571, 1467, 2083, 2418
TspGWI ACGGA 2 cut(s) 1225, 1272
TspRI CASTG 8 cut(s) 580, 657, 942, 1729, 1791, 2031, 2388, 2427
Vha464I CTTAAG 2 cut(s) 1483, 1979
VspI ATTAAT 1 cut(s) 876
XapI RAATTY 2 cut(s) 11, 1217
XbaI TCTAGA 1 cut(s) 2209
XceI RCATGY 5 cut(s) 583, 753, 979, 1247, 1735
XcmI CCANNNNNNNNNTGG 1 cut(s) 892
XmiI GTMKAC 1 cut(s) 2429
XmnI GAANNNNTTC 5 cut(s) 1416, 1443, 1665, 1679, 2402
XspI CTAG 7 cut(s) 584, 1304, 1571, 2069, 2210, 2367, 2645
ZraI GACGTC 1 cut(s) 1163
ZrmI AGTACT 1 cut(s) 1665
Zsp2I ATGCAT 2 cut(s) 1022, 1737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.