Rroxscaffold_1G00039880

UPF0505 protein C16orf62 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007890
Physical Location & Seq
Forward (+)
57550197 .. 57562780
12584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00039880.1

Sequence Viewer

Length: 2829 bp
ATGCTCTCCAACTCCATGGAGTTCAGACCTCGCAATTACACTGCCGAGAAACAAGCCCACGCGCTTCCTCGATCACGCGCCGACGATCATCCTCTCTCCGCTCCATCGTCTCTACCTCACCCCCAGGTTGAGGTTGTTGATGATGGGGACACCGACTATCTTGACCCACTCAGAAGGTCAGATAACAATGCAGCAGTTTCTATAGAGGATTTGGAAGGCATTGAAGACAGTTCTGCTCTGGGACTCTCCAGTGAAGCTTCGATTCAAAATCCAGCAAAAGAATGGGTCTCTTTTAAGAGGTCACTGATGCAGCGGTTTCCGGTCTCCAAAACTGTTTTGACTTCTTCAATGTCCGATGTGATAGTGAAAGGCAGGAAAACATATGACAAATCTTCAACAAATATGCATTTGGAGGAACTGGAAGATCCAGAAAAATTTGCAGAAGAGGGTGGCAAAGTCATCACAGGACAGGAATATGTTTCCCGGTTACACGAGCTCAAAGCTGAAATAAATCACACTTGGCATGCTGATGATCGTGTGACATCTTTGAAATTATCAATAAAAGTAGCTAAGCTTTTGATGGATACGTCTATTTTACAATGTTATCCTACACTTTTTGTTCTTGCTACGGATATTATGGATATGCTTGGCGATATGGTGTGGGAACGCATCAAGTTGAAAGCTGAATTAGCTGAAGATGGAACCAAACTCTGCTTGTTACCAGAGAACTTCATGGCAAGTGATATTTGCTCTGATGCCAAAGAAACTTGCAATAATTGGTTCAGCAAAATTGGTGCAATCCGAGAGCTTCTTCCACGTATTTATTTGGAGCTCGCATTATTGCCTTGCTGGCGTTTCTTGCATGATCGACCTCTAGACTGTCTTCAGCGCTTGGTAATGATGACAAGAGGGTTAGCAGATCCATTGGCATCTGCCTACTGTCGCTTGTATATGGCCCATTGTTTTAGAAAGTTGCACTCTCATGATATAGGATGTCTGCTCACCTGTGTTAATGACTTCAAAATTTTGTTGATGCGGGTGATATCGGCAAAGGAAACATTTCACAGAAACTTGACTGAAAACAAAAGATTGCTTATCAGTCTAATGGAACCAACCATTGATTATATCATGAAATGCATATTTAAAAATGTGTCCGAGAGACAAGTAAATGATGTACTAGTGGAGCTTGGGCTTGGATCTAATCAAGAGGAGCTGCGTGGAAGGTTCCCATGCGCCTCAATTATCCTTCATCATCTAGTCAAGCAACTTCCCTCACAAGTAGTTAGCTCAAGTGCTATGGAGATCATTCGGCTCATTGAGTTCAGCAATGATGATTCCTATGATCAGATCCCTTCCTTTAGGGGTTGGATTAATCGATGTGTTAGGTTACTTGGATTTAGGCTGTGTGAAAGAAAATCTGAAATGGGCACTGTCAATGCTGTGGTGGACAAAGTCATTCAGGTTATTACTCAATATGAAGGCCTTGATGAGTACTTGAAGGTTGTGGATGCTTATGTGGACATTTTCCTTCAAAATCATATGGATGACCACCTGAACTCCATTTTGGAAGGTATTTCAAACCGAGCATGCAGTCAAGGGATTGCTGAAGATGAACTGATGACCTTGCAGTCTATCTTGGTGAAGCTTCTTTCTTATTACGAGGATTTACAAGACGTAATTGCTATGACCCCTTTTCTGAAGATCTTGGATTTAATGCATGGAAGTTCACAAAGCATCGTCAATATGCAGATCCTTGACATGGGGATAAGGAATGGTTGTATACGTGATCGGACAACTATACAGTTTCTTTTTGAAATTTCCCAGGCTCTACATGATGATGAAATTTTTGTGAATGTAAAAGATGATTCTAACCAAGCAGCACGTTTGATTTCTTCTTTTGTCTCACTGGTTGATTATGGGGCAGAGTTGGAAAGTCATTTGACCTTCTTAGTTGAATGCCGTGCAGCTTTTGGTAGCATACACTGGCTTAAGGAAACTCTTGTTCACTTGAGTAATTGTTTAGCAATTAAGGCTCTGAAAGATGGGAATAGACATATCAGTTTTATCAAATCCTGCGTAGCATTTTCTGAAGTCACATTACCTTCTATTTCAGCTCAGACTAAGCAGTTAAATCTGTATCTTGAGACTGCAGAGGTAGCCTTACTTGGTGGTCTAGTTTCTCATTCCGATGGACTGATTGACTCAGCAATCAACTCTTTGCAAATTTCAGAAGCGATGGATGGGTCCAGAACAATTGATGCTGATGGAATACTCTCCTCTATTCAAAAATTATTCAGCCTCTTGGTTATGGTTCCAGGTGGTAATCCTGAGCATGGAATTACTTTCCATCCAAAGAACTTGGTATCACTTGTAAATTCTCAGCCATGGATGACACCAAGAATGAGAGTGAAGATTTTTTGTGCAGTTGTTTCTCTGTTAGCGGCACTATCTCAACAAGATCTGCCTTATCATGCAGATAAGGGAAAGCGTCTAGGAAACGACATACTGTTCTTTGGTCATTCATCGTATTTAAGTGAGCTTGCCTTGATGTGCAAGTTTGTTCTTCAGAACCTGGTTGATACTATTCAACAAGAACCTTCCAAGGCTGCTCGTGGAAGTATGGCTCTTGAAGCTTGCAACTGCATTGCATCATCTTTCATTCCAAGTCAGGAAATATCCTCAATTTGCTCCAATCTAATAGAAACTGGGAAATCGTGTATGGGCACTAGCCACCCGTATCTGCTATCGACGATCAAATTTATGGATCACTTGCTGGTTCCATCTGTTGCGATTACATATCTTGCAGAGAAGCAGTACAGTTCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vps35

Protein Analysis

942

Amino Acids

105.57

Weight (kDa)

5.52

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vps35 PF03635 444 - 728 1.6e-07 Vacuolar protein sorting-associated protein 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1627
AccBSI CCGCTC 1 cut(s) 101
AccI GTMKAC 1 cut(s) 1780
AccII CGCG 2 cut(s) 62, 78
AciI CCGC 4 cut(s) 99, 313, 1036, 2441
AclWI GGATC 6 cut(s) 419, 914, 1204, 1342, 1744, 2772
AcsI RAATTY 7 cut(s) 434, 1023, 1815, 1842, 2223, 2374, 2756
AcuI CTGAAG 6 cut(s) 714, 869, 1626, 1718, 2109, 2549
AfaI GTAC 3 cut(s) 1176, 1493, 2816
AfeI AGCGCT 1 cut(s) 890
AfiI CCNNNNNNNGG 3 cut(s) 130, 1759, 2333
AflII CTTAAG 1 cut(s) 1988
AhlI ACTAGT 1 cut(s) 1177
AjnI CCWGG 4 cut(s) 123, 1821, 2314, 2571
AjuI GAANNNNNNNTTGG 2 cut(s) 1547, 1579
Alw21I GWGCWC 2 cut(s) 498, 834
Alw26I GTCTC 6 cut(s) 114, 292, 328, 1153, 1906, 2138
AlwI GGATC 6 cut(s) 419, 914, 1204, 1342, 1744, 2772
AlwNI CAGNNNCTG 1 cut(s) 2572
Aor51HI AGCGCT 1 cut(s) 890
AoxI GGCC 2 cut(s) 954, 1480
ApeKI GCWGC 6 cut(s) 191, 310, 1213, 1877, 1964, 2606
ApoI RAATTY 7 cut(s) 434, 1023, 1815, 1842, 2223, 2374, 2756
AseI ATTAAT 1 cut(s) 1371
Asp700I GAANNNNTTC 1 cut(s) 1059
AspLEI GCGC 4 cut(s) 64, 80, 891, 1235
AspS9I GGNCC 2 cut(s) 955, 2244
AsuC2I CCSGG 1 cut(s) 484
AsuHPI GGTGA 4 cut(s) 110, 994, 1051, 1651
AvaII GGWCC 1 cut(s) 2244
BaeGI GKGCMC 2 cut(s) 1430, 2726
BanII GRGCYC 2 cut(s) 498, 834
BarI GAAGNNNNNNTAC 2 cut(s) 2082, 2114
BauI CACGAG 2 cut(s) 491, 2610
BbsI GAAGAC 2 cut(s) 231, 875
Bbv12I GWGCWC 2 cut(s) 498, 834
BbvI GCAGC 6 cut(s) 203, 322, 1200, 1889, 1976, 2593
BceAI ACGGC 1 cut(s) 1944
BciT130I CCWGG 4 cut(s) 125, 1823, 2316, 2573
BciVI GTATCC 1 cut(s) 577
BclI TGATCA 1 cut(s) 1342
BcnI CCSGG 1 cut(s) 484
BcoDI GTCTC 6 cut(s) 114, 292, 328, 1153, 1906, 2138
BcuI ACTAGT 1 cut(s) 1177
BfaI CTAG 7 cut(s) 875, 1178, 1256, 2174, 2492, 2727, 2823
BfmI CTRYAG 2 cut(s) 201, 2148
BfoI RGCGCY 1 cut(s) 892
BfrI CTTAAG 1 cut(s) 1988
BfuI GTATCC 1 cut(s) 577
BglI GCCNNNNNGGC 1 cut(s) 850
BglII AGATCT 2 cut(s) 1701, 2458
BisI GCNGC 7 cut(s) 192, 311, 1214, 1878, 1965, 2442, 2607
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 7 cut(s) 193, 312, 1215, 1879, 1966, 2443, 2608
BmcAI AGTACT 1 cut(s) 1493
Bme1390I CCNGG 5 cut(s) 125, 484, 1823, 2316, 2573
Bme18I GGWCC 1 cut(s) 2244
BmgT120I GGNCC 2 cut(s) 955, 2244
BmiI GGNNCC 6 cut(s) 703, 1110, 1226, 2245, 2313, 2778
BmrFI CCNGG 5 cut(s) 125, 484, 1823, 2316, 2573
BmrI ACTGGG 1 cut(s) 2715
BmsI GCATC 9 cut(s) 297, 678, 745, 938, 1023, 1498, 1743, 2248, 2657
BmuI ACTGGG 1 cut(s) 2715
BpiI GAAGAC 2 cut(s) 231, 875
BpmI CTGGAG 1 cut(s) 232
Bpu10I CCTNAGC 1 cut(s) 2328
Bpu1102I GCTNAGC 1 cut(s) 570
BpuEI CTTGAG 3 cut(s) 1273, 2029, 2162
BpuMI CCSGG 1 cut(s) 484
Bsa29I ATCGAT 1 cut(s) 1375
BsaAI YACGTR 2 cut(s) 818, 1784
BsaI GGTCTC 2 cut(s) 292, 328
BsaJI CCNNGG 5 cut(s) 15, 123, 1821, 2384, 2601
BsaWI WCCGGW 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 1541, 1571
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 1759, 2333
Bse1I ACTGG 5 cut(s) 249, 423, 1911, 1988, 2710
Bse3DI GCAATG 2 cut(s) 1333, 2643
BseBI CCWGG 4 cut(s) 125, 1823, 2316, 2573
BseCI ATCGAT 1 cut(s) 1375
BseDI CCNNGG 5 cut(s) 15, 123, 1821, 2384, 2601
BseGI GGATG 7 cut(s) 88, 998, 1513, 1549, 2245, 2347, 2394
BseLI CCNNNNNNNGG 3 cut(s) 130, 1759, 2333
BseMI GCAATG 2 cut(s) 1333, 2643
BseMII CTCAG 5 cut(s) 184, 2129, 2217, 2319, 2393
BseNI ACTGG 5 cut(s) 249, 423, 1911, 1988, 2710
BseRI GAGGAG 2 cut(s) 1223, 2266
BseSI GKGCMC 2 cut(s) 1430, 2726
BseXI GCAGC 6 cut(s) 203, 322, 1200, 1889, 1976, 2593
BsgI GTGCAG 2 cut(s) 1983, 2442
Bsh1236I CGCG 2 cut(s) 62, 78
BshFI GGCC 2 cut(s) 956, 1482
BshVI ATCGAT 1 cut(s) 1375
BsiHKAI GWGCWC 2 cut(s) 498, 834
BsiSI CCGG 2 cut(s) 320, 484
BslFI GGGAC 2 cut(s) 161, 255
BslI CCNNNNNNNGG 3 cut(s) 130, 1759, 2333
BsmAI GTCTC 6 cut(s) 114, 292, 328, 1153, 1906, 2138
BsmBI CGTCTC 1 cut(s) 114
BsmFI GGGAC 2 cut(s) 161, 255
BsmI GAATGC 1 cut(s) 1961
BsnI GGCC 2 cut(s) 956, 1482
Bso31I GGTCTC 2 cut(s) 292, 328
Bsp1286I GDGCHC 4 cut(s) 498, 834, 1430, 2726
Bsp1720I GCTNAGC 1 cut(s) 570
Bsp19I CCATGG 2 cut(s) 15, 2384
BspACI CCGC 4 cut(s) 99, 313, 1036, 2441
BspANI GGCC 2 cut(s) 956, 1482
BspCNI CTCAG 5 cut(s) 183, 2128, 2216, 2320, 2392
BspDI ATCGAT 1 cut(s) 1375
BspFNI CGCG 2 cut(s) 62, 78
BspHI TCATGA 2 cut(s) 982, 1128
BspLI GGNNCC 6 cut(s) 703, 1110, 1226, 2245, 2313, 2778
BspMAI CTGCAG 1 cut(s) 2152
BspPI GGATC 6 cut(s) 419, 914, 1204, 1342, 1744, 2772
BspTI CTTAAG 1 cut(s) 1988
BspTNI GGTCTC 2 cut(s) 292, 328
BsrBI CCGCTC 1 cut(s) 101
BsrDI GCAATG 2 cut(s) 1333, 2643
BsrI ACTGG 5 cut(s) 249, 423, 1911, 1988, 2710
BssECI CCNNGG 5 cut(s) 15, 123, 1821, 2384, 2601
BssNAI GTATAC 1 cut(s) 1781
BssSI CACGAG 2 cut(s) 491, 2610
BssT1I CCWWGG 3 cut(s) 15, 2384, 2601
Bst1107I GTATAC 1 cut(s) 1781
Bst2BI CACGAG 2 cut(s) 491, 2610
Bst2UI CCWGG 4 cut(s) 125, 1823, 2316, 2573
Bst4CI ACNGT 8 cut(s) 230, 334, 881, 941, 1432, 1803, 2508, 2819
Bst6I CTCTTC 1 cut(s) 438
BstAFI CTTAAG 1 cut(s) 1988
BstBAI YACGTR 2 cut(s) 818, 1784
BstC8I GCNNGC 6 cut(s) 525, 834, 851, 1588, 2541, 2635
BstDEI CTNAG 8 cut(s) 170, 570, 1948, 2115, 2121, 2203, 2328, 2379
BstDSI CCRYGG 2 cut(s) 15, 2384
BstF5I GGATG 7 cut(s) 88, 998, 1513, 1549, 2245, 2347, 2394
BstFNI CGCG 2 cut(s) 62, 78
BstH2I RGCGCY 1 cut(s) 892
BstHHI GCGC 4 cut(s) 64, 80, 891, 1235
BstMAI GTCTC 6 cut(s) 114, 292, 328, 1153, 1906, 2138
BstMWI GCNNNNNNNGC 6 cut(s) 689, 850, 859, 2030, 2156, 2630
BstNI CCWGG 4 cut(s) 125, 1823, 2316, 2573
BstNSI RCATGY 2 cut(s) 527, 1590
BstSCI CCNGG 5 cut(s) 123, 482, 1821, 2314, 2571
BstSFI CTRYAG 2 cut(s) 201, 2148
BstSLI GKGCMC 2 cut(s) 1430, 2726
BstUI CGCG 2 cut(s) 62, 78
BstV1I GCAGC 6 cut(s) 203, 322, 1200, 1889, 1976, 2593
BstV2I GAAGAC 2 cut(s) 231, 875
BstX2I RGATCY 7 cut(s) 424, 919, 1196, 1347, 1701, 1749, 2458
BstXI CCANNNNNNTGG 1 cut(s) 16
BstYI RGATCY 7 cut(s) 424, 919, 1196, 1347, 1701, 1749, 2458
BstZ17I GTATAC 1 cut(s) 1781
Bsu15I ATCGAT 1 cut(s) 1375
BsuI GTATCC 1 cut(s) 577
BsuRI GGCC 2 cut(s) 956, 1482
BsuTUI ATCGAT 1 cut(s) 1375
BtgI CCRYGG 2 cut(s) 15, 2384
BtgZI GCGATG 1 cut(s) 2249
BtsCI GGATG 7 cut(s) 88, 998, 1513, 1549, 2245, 2347, 2394
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 6 cut(s) 39, 256, 302, 1428, 1904, 1981
Cac8I GCNNGC 6 cut(s) 525, 834, 851, 1588, 2541, 2635
CaiI CAGNNNCTG 1 cut(s) 2572
CciI TCATGA 2 cut(s) 982, 1128
CfoI GCGC 4 cut(s) 64, 80, 891, 1235
Cfr13I GGNCC 2 cut(s) 955, 2244
ClaI ATCGAT 1 cut(s) 1375
CseI GACGC 1 cut(s) 2477
CsiI ACCWGGT 1 cut(s) 2571
Csp6I GTAC 3 cut(s) 1175, 1492, 2815
CviQI GTAC 3 cut(s) 1175, 1492, 2815
DdeI CTNAG 8 cut(s) 170, 570, 1948, 2115, 2121, 2203, 2328, 2379
DraI TTTAAA 1 cut(s) 1144
DrdI GACNNNNNNGTC 1 cut(s) 1627
DseDI GACNNNNNNGTC 1 cut(s) 1627
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Ecl136II GAGCTC 2 cut(s) 496, 832
Eco130I CCWWGG 3 cut(s) 15, 2384, 2601
Eco147I AGGCCT 1 cut(s) 1482
Eco24I GRGCYC 2 cut(s) 498, 834
Eco31I GGTCTC 2 cut(s) 292, 328
Eco32I GATATC 1 cut(s) 1044
Eco47I GGWCC 1 cut(s) 2244
Eco47III AGCGCT 1 cut(s) 890
Eco53kI GAGCTC 2 cut(s) 496, 832
Eco57I CTGAAG 6 cut(s) 714, 869, 1626, 1718, 2109, 2549
EcoICRI GAGCTC 2 cut(s) 496, 832
EcoRII CCWGG 4 cut(s) 123, 1821, 2314, 2571
EcoRV GATATC 1 cut(s) 1044
EcoT14I CCWWGG 3 cut(s) 15, 2384, 2601
EcoT22I ATGCAT 3 cut(s) 408, 1139, 1719
EcoT38I GRGCYC 2 cut(s) 498, 834
ErhI CCWWGG 3 cut(s) 15, 2384, 2601
Esp3I CGTCTC 1 cut(s) 114
FaqI GGGAC 2 cut(s) 161, 255
FauI CCCGC 1 cut(s) 1029
FauNDI CATATG 2 cut(s) 382, 1539
FbaI TGATCA 1 cut(s) 1342
FblI GTMKAC 1 cut(s) 1780
Fnu4HI GCNGC 7 cut(s) 192, 311, 1214, 1878, 1965, 2442, 2607
FokI GGATG 7 cut(s) 75, 1005, 1520, 1556, 2252, 2334, 2401
FriOI GRGCYC 2 cut(s) 498, 834
Fsp4HI GCNGC 7 cut(s) 192, 311, 1214, 1878, 1965, 2442, 2607
FspBI CTAG 7 cut(s) 875, 1178, 1256, 2174, 2492, 2727, 2823
GlaI GCGC 4 cut(s) 63, 79, 890, 1234
GluI GCNGC 7 cut(s) 192, 311, 1214, 1878, 1965, 2442, 2607
GsuI CTGGAG 1 cut(s) 232
HaeII RGCGCY 1 cut(s) 892
HaeIII GGCC 2 cut(s) 956, 1482
HapII CCGG 2 cut(s) 320, 484
HgaI GACGC 1 cut(s) 2477
HhaI GCGC 4 cut(s) 64, 80, 891, 1235
Hin6I GCGC 4 cut(s) 62, 78, 889, 1233
HinP1I GCGC 4 cut(s) 62, 78, 889, 1233
HindIII AAGCTT 4 cut(s) 255, 572, 1643, 2631
HinfI GANTC 5 cut(s) 243, 262, 1334, 1865, 2201
HpaII CCGG 2 cut(s) 320, 484
HphI GGTGA 4 cut(s) 110, 994, 1051, 1651
Hpy166II GTNNAC 5 cut(s) 1447, 1519, 1727, 1781, 2005
Hpy8I GTNNAC 5 cut(s) 1447, 1519, 1727, 1781, 2005
Hpy99I CGWCG 2 cut(s) 86, 2752
HpyCH4III ACNGT 8 cut(s) 230, 334, 881, 941, 1432, 1803, 2508, 2819
HpyCH4IV ACGT 5 cut(s) 587, 817, 1674, 1783, 1882
HpyF10VI GCNNNNNNNGC 6 cut(s) 689, 850, 859, 2030, 2156, 2630
HpyF3I CTNAG 8 cut(s) 170, 570, 1948, 2115, 2121, 2203, 2328, 2379
HpySE526I ACGT 5 cut(s) 587, 817, 1674, 1783, 1882
HspAI GCGC 4 cut(s) 62, 78, 889, 1233
Ksp22I TGATCA 1 cut(s) 1342
LmnI GCTCC 5 cut(s) 106, 829, 1183, 1210, 2693
Lsp1109I GCAGC 6 cut(s) 203, 322, 1200, 1889, 1976, 2593
LweI GCATC 9 cut(s) 297, 678, 745, 938, 1023, 1498, 1743, 2248, 2657
MabI ACCWGGT 1 cut(s) 2571
MaeI CTAG 7 cut(s) 875, 1178, 1256, 2174, 2492, 2727, 2823
MaeII ACGT 5 cut(s) 587, 817, 1674, 1783, 1882
MaeIII GTNAC 6 cut(s) 300, 486, 538, 717, 1386, 2092
MbiI CCGCTC 1 cut(s) 101
MfeI CAATTG 1 cut(s) 2253
MflI RGATCY 7 cut(s) 424, 919, 1196, 1347, 1701, 1749, 2458
MhlI GDGCHC 4 cut(s) 498, 834, 1430, 2726
MlyI GAGTC 2 cut(s) 237, 2195
MmeI TCCRAC 3 cut(s) 33, 1346, 1908
Mph1103I ATGCAT 3 cut(s) 408, 1139, 1719
MroXI GAANNNNTTC 1 cut(s) 1059
MseI TTAA 9 cut(s) 294, 1011, 1143, 1371, 1712, 1989, 2028, 2129, 2531
MslI CAYNNNNRTG 5 cut(s) 528, 981, 1542, 1836, 2187
MspA1I CMGCKG 1 cut(s) 313
MspCI CTTAAG 1 cut(s) 1988
MspI CCGG 2 cut(s) 320, 484
MspR9I CCNGG 5 cut(s) 125, 484, 1823, 2316, 2573
MunI CAATTG 1 cut(s) 2253
Mva1269I GAATGC 1 cut(s) 1961
MvaI CCWGG 4 cut(s) 125, 1823, 2316, 2573
MvnI CGCG 2 cut(s) 62, 78
MwoI GCNNNNNNNGC 6 cut(s) 689, 850, 859, 2030, 2156, 2630
NciI CCSGG 1 cut(s) 484
NcoI CCATGG 2 cut(s) 15, 2384
NdeI CATATG 2 cut(s) 382, 1539
NlaIV GGNNCC 6 cut(s) 703, 1110, 1226, 2245, 2313, 2778
NmeAIII GCCGAG 1 cut(s) 70
NmuCI GTSAC 3 cut(s) 300, 538, 2092
NsiI ATGCAT 3 cut(s) 408, 1139, 1719
NspI RCATGY 2 cut(s) 527, 1590
PaeI GCATGC 2 cut(s) 527, 1590
PagI TCATGA 2 cut(s) 982, 1128
PceI AGGCCT 1 cut(s) 1482
PctI GAATGC 1 cut(s) 1961
PdmI GAANNNNTTC 1 cut(s) 1059
PfeI GAWTC 3 cut(s) 262, 1334, 1865
PflFI GACNNNGTC 1 cut(s) 1451
PkrI GCNGC 7 cut(s) 193, 312, 1215, 1879, 1966, 2443, 2608
PleI GAGTC 2 cut(s) 237, 2195
PpsI GAGTC 2 cut(s) 237, 2195
Ppu21I YACGTR 2 cut(s) 818, 1784
PshBI ATTAAT 1 cut(s) 1371
Psp124BI GAGCTC 2 cut(s) 498, 834
Psp6I CCWGG 4 cut(s) 123, 1821, 2314, 2571
PspGI CCWGG 4 cut(s) 123, 1821, 2314, 2571
PspN4I GGNNCC 6 cut(s) 703, 1110, 1226, 2245, 2313, 2778
PspPI GGNCC 2 cut(s) 955, 2244
PstI CTGCAG 1 cut(s) 2152
PstNI CAGNNNCTG 1 cut(s) 2572
PsuI RGATCY 7 cut(s) 424, 919, 1196, 1347, 1701, 1749, 2458
PsyI GACNNNGTC 1 cut(s) 1451
RsaI GTAC 3 cut(s) 1176, 1493, 2816
RsaNI GTAC 3 cut(s) 1175, 1492, 2815
RseI CAYNNNNRTG 5 cut(s) 528, 981, 1542, 1836, 2187
SacI GAGCTC 2 cut(s) 498, 834
SaqAI TTAA 9 cut(s) 294, 1011, 1143, 1371, 1712, 1989, 2028, 2129, 2531
SatI GCNGC 7 cut(s) 192, 311, 1214, 1878, 1965, 2442, 2607
Sau96I GGNCC 2 cut(s) 955, 2244
ScaI AGTACT 1 cut(s) 1493
SchI GAGTC 2 cut(s) 237, 2195
ScrFI CCNGG 5 cut(s) 125, 484, 1823, 2316, 2573
SduI GDGCHC 4 cut(s) 498, 834, 1430, 2726
SexAI ACCWGGT 1 cut(s) 2571
SfaNI GCATC 9 cut(s) 297, 678, 745, 938, 1023, 1498, 1743, 2248, 2657
SfcI CTRYAG 2 cut(s) 201, 2148
SinI GGWCC 1 cut(s) 2244
SmiMI CAYNNNNRTG 5 cut(s) 528, 981, 1542, 1836, 2187
SmlI CTYRAG 4 cut(s) 1288, 1988, 2008, 2141
SmoI CTYRAG 4 cut(s) 1288, 1988, 2008, 2141
SpeI ACTAGT 1 cut(s) 1177
SphI GCATGC 2 cut(s) 527, 1590
SseBI AGGCCT 1 cut(s) 1482
SsiI CCGC 4 cut(s) 99, 313, 1036, 2441
SspMI CTAG 7 cut(s) 875, 1178, 1256, 2174, 2492, 2727, 2823
SstI GAGCTC 2 cut(s) 498, 834
StuI AGGCCT 1 cut(s) 1482
StyD4I CCNGG 5 cut(s) 123, 482, 1821, 2314, 2571
StyI CCWWGG 3 cut(s) 15, 2384, 2601
TaaI ACNGT 8 cut(s) 230, 334, 881, 941, 1432, 1803, 2508, 2819
TaiI ACGT 5 cut(s) 590, 820, 1677, 1786, 1885
TaqI TCGA 5 cut(s) 70, 260, 868, 1375, 2747
TatI WGTACW 3 cut(s) 1174, 1491, 2814
TauI GCSGC 1 cut(s) 2444
TfiI GAWTC 3 cut(s) 262, 1334, 1865
Tru1I TTAA 9 cut(s) 294, 1011, 1143, 1371, 1712, 1989, 2028, 2129, 2531
Tru9I TTAA 9 cut(s) 294, 1011, 1143, 1371, 1712, 1989, 2028, 2129, 2531
TscAI CASTG 6 cut(s) 46, 256, 309, 1435, 1911, 1988
TseFI GTSAC 3 cut(s) 300, 538, 2092
TseI GCWGC 6 cut(s) 191, 310, 1213, 1877, 1964, 2606
Tsp45I GTSAC 3 cut(s) 300, 538, 2092
TspDTI ATGAA 8 cut(s) 721, 1145, 1238, 1491, 1626, 1854, 2511, 2647
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 6 cut(s) 46, 256, 309, 1435, 1911, 1988
Tth111I GACNNNGTC 1 cut(s) 1451
Vha464I CTTAAG 1 cut(s) 1988
VpaK11BI GGWCC 1 cut(s) 2244
VspI ATTAAT 1 cut(s) 1371
XapI RAATTY 7 cut(s) 434, 1023, 1815, 1842, 2223, 2374, 2756
XbaI TCTAGA 1 cut(s) 874
XceI RCATGY 2 cut(s) 527, 1590
XcmI CCANNNNNNNNNTGG 1 cut(s) 279
XmiI GTMKAC 1 cut(s) 1780
XmnI GAANNNNTTC 1 cut(s) 1059
XspI CTAG 7 cut(s) 875, 1178, 1256, 2174, 2492, 2727, 2823
ZrmI AGTACT 1 cut(s) 1493
Zsp2I ATGCAT 3 cut(s) 408, 1139, 1719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.