AT1G51310

tRNA methyl transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009292
Physical Location & Seq
Reverse (-)
19018941 .. 19021808
2868 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G51310.1

Sequence Viewer

Length: 1494 bp
ATGTTCTCCGCCGTCGCCAAACCCTTCCTCTCTCCCTCACCACCGCTATTTCGCCTTCCTCTTTCCTCTATCTCAATTTCCTTCCGCGTTTTCCCCTCATTCTTCTCCTCCTCCTCTTCCTCTCAATTGCCGTCTCCACAATCTTCTAACCCAATCACGCCTCTCATATCCTCGAACCGTAACTATAGCCGCCATGCCTTTATCGTCGACCAATACCTCTCATGTTCCATGCCTGAGAAGCCTCTTCGTGTCGCCGTACTCCTCAGTGGCGGCGTCGATAGCAGTGTCGCTCTCCGTCTCCTCCACGCCGCTGGTCACTCATGTACCGCTTTCTATCTCAAAATCTGGTTTCAGGAAGGCTTTGAGAATTTCTGGAATCAGTGCCCTTGGGAAGATGATTTGAAATATGCAAAACATGTTTGTGAGCAGGTTGATGTTCCTTTGGAGGTTGTGCATTTAACAGATGAATATTGGGAAAGAGTTGTTTCCTACATTATTGAGGAGTATCGATGTGGACGTACACCTAATCCGGATGTTCTATGTAATACTAGAATTAAGTTTGGTGCATTCATGGATGCAATCAGTGATATGGAGTATGACTATGTAGGTTCAGGTCATTATGCTAAAGTGGTCCATCCTCCTGCTGACCAAAATGATGCATCATCTGTTTTGGAACTATCACAAGACATGGTGAAGGATCAGACCTACTTCCTCTCACATCTCTCTCAAACCCAGCTTAAGCGACTGCTTTTCCCACTTGGTTGTGTAAAAAAGGACGAAGTTCGCAAACTAGCCACACAATTCGATTTGCCAAATAAAGATAGAAAGGATTCGCAAGGAATTTGTTTCCTTGGGAAGATAAAGTTCAGCGACTTTGTCTGTAGACACATAGGGGAGATGGAAGGAATTATATTGGAAGCCGAATCTGGGGATTTTCTTGGTAACCACCGGGGATTTTGGTTTTACACAATTGGACAACGCCAAGGCTTGCGTTTACCCGGTGGCCCCTGGTATGTTGTTGAAAAGGACACCAAGAACAATGTAGTGTTTGTCTCCAGAAATTATTACTCAATCGACAAGAGAAGGCGGATTTTCCGTGTCGGCTCCTTAAGATGGAATAGTGGGAAACCTTCAGGCAAAGTTAGAGAGCTCCGTTGCAAGGTCCGACATGGGCCTGGCTTCTACAGCTGCAGTTTTGAAATGGAAGGAGACGGAGATGTTGCAGTTGTGCACCTAGACGAAGACGATCAAGGTCTAGCTGCTGGACAGTTTGCAGCGTTTTACGAGGGAACCACTTGCATTGGTTCAGGCGTAATTCTGGAGTCATGGGATGATCAGTGTTTCCCGGTCTGTGCAAAAGCCCTTCAACTCGCAGCATTGGAAGATAAAACCAAACTCGGGAAACCTGTCAAAATCATGACAATGCCTACTATGTCATCTGTTGAGGCTGATACAGGAGAAGCCAGTGCAGAGCAAAAACTAGTCAACGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_Me_trans

Protein Analysis

497

Amino Acids

55.82

Weight (kDa)

6.21

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_Me_trans PF03054 83 - 286 5.9e-79 tRNA methyl transferase HUP domain
tRNA_Me_trans_M PF20259 291 - 353 2e-25 tRNA methyl transferase PRC-barrel domain
tRNA_Me_trans_C PF20258 363 - 439 4e-21 Aminomethyltransferase beta-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 418
AccI GTMKAC 2 cut(s) 207, 883
AccII CGCG 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 529
AciI CCGC 8 cut(s) 9, 44, 85, 190, 270, 309, 327, 1087
AclWI GGATC 1 cut(s) 705
AcsI RAATTY 2 cut(s) 367, 840
AcuI CTGAAG 1 cut(s) 1116
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 3 cut(s) 258, 325, 520
AfiI CCNNNNNNNGG 4 cut(s) 927, 1113, 1159, 1398
AflII CTTAAG 2 cut(s) 737, 1108
AflIII ACRYGT 1 cut(s) 415
AgsI TTSAA 4 cut(s) 403, 1022, 1199, 1367
AhlI ACTAGT 1 cut(s) 1480
AjnI CCWGG 2 cut(s) 1007, 1174
AluBI AGCT 4 cut(s) 736, 1150, 1188, 1259
AluI AGCT 4 cut(s) 736, 1150, 1188, 1259
Alw21I GWGCWC 2 cut(s) 1152, 1233
Alw26I GTCTC 4 cut(s) 138, 302, 1057, 1203
Alw44I GTGCAC 1 cut(s) 1229
AlwI GGATC 1 cut(s) 705
Ama87I CYCGRG 1 cut(s) 1397
Aor13HI TCCGGA 1 cut(s) 529
AoxI GGCC 2 cut(s) 1003, 1172
ApaLI GTGCAC 1 cut(s) 1229
ApeKI GCWGC 4 cut(s) 1188, 1259, 1274, 1373
ApoI RAATTY 2 cut(s) 367, 840
Asp700I GAANNNNTTC 1 cut(s) 829
AspS9I GGNCC 4 cut(s) 631, 1004, 1162, 1172
AsuC2I CCSGG 3 cut(s) 950, 999, 1346
AsuHPI GGTGA 2 cut(s) 30, 703
AvaI CYCGRG 1 cut(s) 1397
AvaII GGWCC 2 cut(s) 631, 1162
BaeGI GKGCMC 2 cut(s) 386, 1233
BaeI ACNNNNGTAYC 2 cut(s) 307, 340
BanII GRGCYC 1 cut(s) 1152
BbsI GAAGAC 1 cut(s) 1248
Bbv12I GWGCWC 2 cut(s) 1152, 1233
BbvI GCAGC 4 cut(s) 1175, 1246, 1286, 1385
BccI CCATC 3 cut(s) 642, 892, 1107
BceAI ACGGC 2 cut(s) 115, 239
BciT130I CCWGG 2 cut(s) 1009, 1176
BclI TGATCA 1 cut(s) 1333
BcnI CCSGG 3 cut(s) 950, 999, 1346
BcoDI GTCTC 4 cut(s) 138, 302, 1057, 1203
BcuI ACTAGT 1 cut(s) 1480
BfaI CTAG 5 cut(s) 549, 791, 1235, 1256, 1481
BfmI CTRYAG 4 cut(s) 184, 880, 1183, 1189
BfrI CTTAAG 2 cut(s) 737, 1108
BfuAI ACCTGC 1 cut(s) 418
BisI GCNGC 7 cut(s) 190, 271, 309, 1189, 1260, 1275, 1374
BlsI GCNGC 7 cut(s) 191, 272, 310, 1190, 1261, 1276, 1375
Bme1390I CCNGG 5 cut(s) 950, 999, 1009, 1176, 1346
Bme18I GGWCC 2 cut(s) 631, 1162
BmeT110I CYCGRG 1 cut(s) 1397
BmgT120I GGNCC 4 cut(s) 631, 1004, 1162, 1172
BmiI GGNNCC 3 cut(s) 1006, 1105, 1291
BmrFI CCNGG 5 cut(s) 950, 999, 1009, 1176, 1346
BmsI GCATC 3 cut(s) 565, 646, 668
BpiI GAAGAC 1 cut(s) 1248
BpmI CTGGAG 2 cut(s) 1039, 1340
BpuMI CCSGG 3 cut(s) 950, 999, 1346
Bsa29I ATCGAT 1 cut(s) 508
BsaHI GRCGYC 1 cut(s) 273
BsaJI CCNNGG 5 cut(s) 386, 850, 949, 982, 1007
BsaWI WCCGGW 1 cut(s) 529
Bsc4I CCNNNNNNNGG 4 cut(s) 927, 1113, 1159, 1398
Bse1I ACTGG 1 cut(s) 1464
BseAI TCCGGA 1 cut(s) 529
BseBI CCWGG 2 cut(s) 1009, 1176
BseCI ATCGAT 1 cut(s) 508
BseDI CCNNGG 5 cut(s) 386, 850, 949, 982, 1007
BseGI GGATG 4 cut(s) 538, 580, 634, 1336
BseLI CCNNNNNNNGG 4 cut(s) 927, 1113, 1159, 1398
BseMII CTCAG 2 cut(s) 225, 277
BseNI ACTGG 1 cut(s) 1464
BseRI GAGGAG 6 cut(s) 97, 100, 103, 251, 290, 515
BseSI GKGCMC 2 cut(s) 386, 1233
BseXI GCAGC 4 cut(s) 1175, 1246, 1286, 1385
BseYI CCCAGC 1 cut(s) 732
BsgI GTGCAG 1 cut(s) 1488
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 2 cut(s) 1005, 1174
BshVI ATCGAT 1 cut(s) 508
BsiHKAI GWGCWC 2 cut(s) 1152, 1233
BsiHKCI CYCGRG 1 cut(s) 1397
BsiSI CCGG 4 cut(s) 530, 949, 999, 1346
BslI CCNNNNNNNGG 4 cut(s) 927, 1113, 1159, 1398
BsmAI GTCTC 4 cut(s) 138, 302, 1057, 1203
BsmBI CGTCTC 3 cut(s) 138, 302, 1203
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 2 cut(s) 1005, 1174
BsoBI CYCGRG 1 cut(s) 1397
Bsp1286I GDGCHC 3 cut(s) 386, 1152, 1233
Bsp13I TCCGGA 1 cut(s) 529
Bsp143I GATC 3 cut(s) 697, 1246, 1333
BspACI CCGC 8 cut(s) 9, 44, 85, 190, 270, 309, 327, 1087
BspANI GGCC 2 cut(s) 1005, 1174
BspCNI CTCAG 2 cut(s) 226, 276
BspDI ATCGAT 1 cut(s) 508
BspEI TCCGGA 1 cut(s) 529
BspFNI CGCG 1 cut(s) 87
BspHI TCATGA 1 cut(s) 1416
BspLI GGNNCC 3 cut(s) 1006, 1105, 1291
BspMAI CTGCAG 1 cut(s) 1193
BspMI ACCTGC 1 cut(s) 418
BspPI GGATC 1 cut(s) 705
BspTI CTTAAG 2 cut(s) 737, 1108
BsrI ACTGG 1 cut(s) 1464
BssECI CCNNGG 5 cut(s) 386, 850, 949, 982, 1007
BssMI GATC 3 cut(s) 697, 1246, 1333
BssNI GRCGYC 1 cut(s) 273
BssT1I CCWWGG 3 cut(s) 386, 850, 982
Bst2UI CCWGG 2 cut(s) 1009, 1176
Bst4CI ACNGT 2 cut(s) 179, 1269
Bst6I CTCTTC 2 cut(s) 121, 249
BstACI GRCGYC 1 cut(s) 273
BstAFI CTTAAG 2 cut(s) 737, 1108
BstC8I GCNNGC 1 cut(s) 989
BstDEI CTNAG 2 cut(s) 234, 263
BstEII GGTNACC 1 cut(s) 941
BstF5I GGATG 4 cut(s) 538, 580, 634, 1336
BstFNI CGCG 1 cut(s) 87
BstKTI GATC 3 cut(s) 700, 1249, 1336
BstMAI GTCTC 4 cut(s) 138, 302, 1057, 1203
BstMBI GATC 3 cut(s) 697, 1246, 1333
BstMWI GCNNNNNNNGC 3 cut(s) 238, 279, 1185
BstNI CCWGG 2 cut(s) 1009, 1176
BstNSI RCATGY 1 cut(s) 419
BstPI GGTNACC 1 cut(s) 941
BstSCI CCNGG 5 cut(s) 948, 997, 1007, 1174, 1344
BstSFI CTRYAG 4 cut(s) 184, 880, 1183, 1189
BstSLI GKGCMC 2 cut(s) 386, 1233
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 4 cut(s) 1175, 1246, 1286, 1385
BstV2I GAAGAC 1 cut(s) 1248
BstXI CCANNNNNNTGG 1 cut(s) 311
Bsu15I ATCGAT 1 cut(s) 508
BsuRI GGCC 2 cut(s) 1005, 1174
BsuTUI ATCGAT 1 cut(s) 508
BtsCI GGATG 4 cut(s) 538, 580, 634, 1336
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 6 cut(s) 271, 289, 386, 589, 1343, 1471
BveI ACCTGC 1 cut(s) 418
Cac8I GCNNGC 1 cut(s) 989
CciI TCATGA 1 cut(s) 1416
Cfr13I GGNCC 4 cut(s) 631, 1004, 1162, 1172
ClaI ATCGAT 1 cut(s) 508
CseI GACGC 1 cut(s) 262
Csp6I GTAC 3 cut(s) 257, 324, 519
CspCI CAANNNNNGTGG 2 cut(s) 1282, 1317
CviQI GTAC 3 cut(s) 257, 324, 519
DdeI CTNAG 2 cut(s) 234, 263
DpnI GATC 3 cut(s) 699, 1248, 1335
DpnII GATC 3 cut(s) 697, 1246, 1333
Eam1104I CTCTTC 2 cut(s) 121, 249
EarI CTCTTC 2 cut(s) 121, 249
EciI GGCGGA 1 cut(s) 1102
Ecl136II GAGCTC 1 cut(s) 1150
Eco130I CCWWGG 3 cut(s) 386, 850, 982
Eco24I GRGCYC 1 cut(s) 1152
Eco47I GGWCC 2 cut(s) 631, 1162
Eco53kI GAGCTC 1 cut(s) 1150
Eco57I CTGAAG 1 cut(s) 1116
Eco88I CYCGRG 1 cut(s) 1397
Eco91I GGTNACC 1 cut(s) 941
EcoICRI GAGCTC 1 cut(s) 1150
EcoO65I GGTNACC 1 cut(s) 941
EcoRII CCWGG 2 cut(s) 1007, 1174
EcoT14I CCWWGG 3 cut(s) 386, 850, 982
EcoT22I ATGCAT 1 cut(s) 661
EcoT38I GRGCYC 1 cut(s) 1152
ErhI CCWWGG 3 cut(s) 386, 850, 982
Esp3I CGTCTC 3 cut(s) 138, 302, 1203
FbaI TGATCA 1 cut(s) 1333
FblI GTMKAC 2 cut(s) 207, 883
Fnu4HI GCNGC 7 cut(s) 190, 271, 309, 1189, 1260, 1275, 1374
FokI GGATG 4 cut(s) 545, 587, 621, 1343
FriOI GRGCYC 1 cut(s) 1152
Fsp4HI GCNGC 7 cut(s) 190, 271, 309, 1189, 1260, 1275, 1374
FspBI CTAG 5 cut(s) 549, 791, 1235, 1256, 1481
GluI GCNGC 7 cut(s) 190, 271, 309, 1189, 1260, 1275, 1374
GsaI CCCAGC 1 cut(s) 736
GsuI CTGGAG 2 cut(s) 1039, 1340
HaeIII GGCC 2 cut(s) 1005, 1174
HapII CCGG 4 cut(s) 530, 949, 999, 1346
HgaI GACGC 1 cut(s) 262
Hin1I GRCGYC 1 cut(s) 273
HincII GTYRAC 2 cut(s) 208, 1486
HindII GTYRAC 2 cut(s) 208, 1486
HinfI GANTC 4 cut(s) 376, 830, 923, 1322
HpaII CCGG 4 cut(s) 530, 949, 999, 1346
HphI GGTGA 2 cut(s) 30, 703
Hpy166II GTNNAC 7 cut(s) 208, 515, 521, 884, 995, 1231, 1486
Hpy188I TCNGA 2 cut(s) 702, 1166
Hpy188III TCNNGA 7 cut(s) 353, 373, 530, 1056, 1319, 1399, 1417
Hpy8I GTNNAC 7 cut(s) 208, 515, 521, 884, 995, 1231, 1486
Hpy99I CGWCG 3 cut(s) 17, 209, 278
HpyCH4III ACNGT 2 cut(s) 179, 1269
HpyCH4IV ACGT 1 cut(s) 517
HpyF10VI GCNNNNNNNGC 3 cut(s) 238, 279, 1185
HpyF3I CTNAG 2 cut(s) 234, 263
HpySE526I ACGT 1 cut(s) 517
Hsp92I GRCGYC 1 cut(s) 273
Kpn2I TCCGGA 1 cut(s) 529
Ksp22I TGATCA 1 cut(s) 1333
Kzo9I GATC 3 cut(s) 697, 1246, 1333
LmnI GCTCC 2 cut(s) 1109, 1155
Lsp1109I GCAGC 4 cut(s) 1175, 1246, 1286, 1385
LweI GCATC 3 cut(s) 565, 646, 668
MaeI CTAG 5 cut(s) 549, 791, 1235, 1256, 1481
MaeII ACGT 1 cut(s) 517
MaeIII GTNAC 3 cut(s) 179, 314, 941
MalI GATC 3 cut(s) 699, 1248, 1335
MboI GATC 3 cut(s) 697, 1246, 1333
MboII GAAGA 8 cut(s) 94, 108, 135, 236, 404, 868, 1253, 1394
MfeI CAATTG 2 cut(s) 125, 969
MhlI GDGCHC 3 cut(s) 386, 1152, 1233
MlyI GAGTC 1 cut(s) 1331
MmeI TCCRAC 1 cut(s) 1189
Mph1103I ATGCAT 1 cut(s) 661
MroI TCCGGA 1 cut(s) 529
MroXI GAANNNNTTC 1 cut(s) 829
MseI TTAA 4 cut(s) 458, 555, 738, 1109
MslI CAYNNNNRTG 2 cut(s) 420, 1421
MspA1I CMGCKG 2 cut(s) 311, 1188
MspCI CTTAAG 2 cut(s) 737, 1108
MspI CCGG 4 cut(s) 530, 949, 999, 1346
MspR9I CCNGG 5 cut(s) 950, 999, 1009, 1176, 1346
MunI CAATTG 2 cut(s) 125, 969
Mva1269I GAATGC 1 cut(s) 566
MvaI CCWGG 2 cut(s) 1009, 1176
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 3 cut(s) 238, 279, 1185
NciI CCSGG 3 cut(s) 950, 999, 1346
NdeII GATC 3 cut(s) 697, 1246, 1333
NlaIV GGNNCC 3 cut(s) 1006, 1105, 1291
NmuCI GTSAC 1 cut(s) 314
NsiI ATGCAT 1 cut(s) 661
NspI RCATGY 1 cut(s) 419
PagI TCATGA 1 cut(s) 1416
PciI ACATGT 1 cut(s) 415
PcsI WCGNNNNNNNCGW 1 cut(s) 514
PctI GAATGC 1 cut(s) 566
PdmI GAANNNNTTC 1 cut(s) 829
PfeI GAWTC 3 cut(s) 376, 830, 923
PflFI GACNNNGTC 1 cut(s) 875
PkrI GCNGC 7 cut(s) 191, 272, 310, 1190, 1261, 1276, 1375
PleI GAGTC 1 cut(s) 1330
PpsI GAGTC 1 cut(s) 1330
PscI ACATGT 1 cut(s) 415
Psp124BI GAGCTC 1 cut(s) 1152
Psp6I CCWGG 2 cut(s) 1007, 1174
PspEI GGTNACC 1 cut(s) 941
PspFI CCCAGC 1 cut(s) 732
PspGI CCWGG 2 cut(s) 1007, 1174
PspN4I GGNNCC 3 cut(s) 1006, 1105, 1291
PspPI GGNCC 4 cut(s) 631, 1004, 1162, 1172
PstI CTGCAG 1 cut(s) 1193
PsyI GACNNNGTC 1 cut(s) 875
PvuII CAGCTG 1 cut(s) 1188
RsaI GTAC 3 cut(s) 258, 325, 520
RsaNI GTAC 3 cut(s) 257, 324, 519
RseI CAYNNNNRTG 2 cut(s) 420, 1421
SacI GAGCTC 1 cut(s) 1152
SalI GTCGAC 1 cut(s) 206
SaqAI TTAA 4 cut(s) 458, 555, 738, 1109
SatI GCNGC 7 cut(s) 190, 271, 309, 1189, 1260, 1275, 1374
Sau3AI GATC 3 cut(s) 697, 1246, 1333
Sau96I GGNCC 4 cut(s) 631, 1004, 1162, 1172
SchI GAGTC 1 cut(s) 1331
ScrFI CCNGG 5 cut(s) 950, 999, 1009, 1176, 1346
SduI GDGCHC 3 cut(s) 386, 1152, 1233
SfaNI GCATC 3 cut(s) 565, 646, 668
SfcI CTRYAG 4 cut(s) 184, 880, 1183, 1189
SinI GGWCC 2 cut(s) 631, 1162
SmiMI CAYNNNNRTG 2 cut(s) 420, 1421
SmlI CTYRAG 2 cut(s) 737, 1108
SmoI CTYRAG 2 cut(s) 737, 1108
SpeI ACTAGT 1 cut(s) 1480
SsiI CCGC 8 cut(s) 9, 44, 85, 190, 270, 309, 327, 1087
SspI AATATT 1 cut(s) 470
SspMI CTAG 5 cut(s) 549, 791, 1235, 1256, 1481
SstI GAGCTC 1 cut(s) 1152
StyD4I CCNGG 5 cut(s) 948, 997, 1007, 1174, 1344
StyI CCWWGG 3 cut(s) 386, 850, 982
TaaI ACNGT 2 cut(s) 179, 1269
TaiI ACGT 1 cut(s) 520
TaqI TCGA 6 cut(s) 173, 207, 276, 508, 804, 1074
TauI GCSGC 3 cut(s) 192, 273, 311
TfiI GAWTC 3 cut(s) 376, 830, 923
Tru1I TTAA 4 cut(s) 458, 555, 738, 1109
Tru9I TTAA 4 cut(s) 458, 555, 738, 1109
TscAI CASTG 6 cut(s) 271, 289, 386, 589, 1343, 1471
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 4 cut(s) 1188, 1259, 1274, 1373
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 2 cut(s) 480, 559
TspGWI ACGGA 4 cut(s) 284, 1085, 1142, 1227
TspRI CASTG 6 cut(s) 271, 289, 386, 589, 1343, 1471
Tth111I GACNNNGTC 1 cut(s) 875
Vha464I CTTAAG 2 cut(s) 737, 1108
VneI GTGCAC 1 cut(s) 1229
VpaK11BI GGWCC 2 cut(s) 631, 1162
XapI RAATTY 2 cut(s) 367, 840
XceI RCATGY 1 cut(s) 419
XmiI GTMKAC 2 cut(s) 207, 883
XmnI GAANNNNTTC 1 cut(s) 829
XspI CTAG 5 cut(s) 549, 791, 1235, 1256, 1481
Zsp2I ATGCAT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.