Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0009292

Physical Location & Seq

Genomic Coordinates Forward (+)

24188657 .. 24195461

Gene Structure 6805 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1176400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1569 bp

ATGGCGAGAGTAATACCAGTGGCTATGGCGGCCTCTCTACTCCCCCACATCACTTCGCTCTCTCTCCCTCTCAAGCCCTATCTCTCGCTGACAAGTCTCCTCTTCCTCAGACCCTTCTCTCGCTCGTGCGCCGACTGCTCCCCCCGCTCCGTTCACTGCTTCGCCGTTCCCCAAACTCCACGCCCTCCAAACAGCTTGAATGGAGATGTTGGCCCTTACTTGTCCTGTTCTATGCCTCATCAGAGCCTTAAAGTCGCCGTCTTGCTTAGCGGCGGTGTCGATAGCAGTGTCGCCCTCCGCCTCCTCCATGCCGCCGGTCACTCCTGCACCGCCTTCTACCTCAAAATTTGGTTCCAAGAAGACTTTGAGAACTTTTGGTCAGAATGCCCATGGGAAGAAGATTTGAAGTATGCAAATGCTGTCTGCAATCAGGTTGGTGTCCCACTAGAAGTCGTGCATTTGACGGATGAATATTGGAAGAATGTGGTATCCTACATTATTAAAGACTATCAATGTGGCAGAACTCCTAACCCAGATGTTCTTTGCAATACAAGAATAAAGTTTGGTGCATTCATGGATGCCATCAGCAGTATGAAGTTTGACCATGTGGCATCTGGGCATTACGCAAATGTTGTCCACCCACCTGCAAGTCAAACGGACAAGGATTCGGTTCTAGAACTATCACAAGATATGGTGAAGGATCAGACATACTTCCTTTCACATCTTTCTCAGGCCCAGCTTAAGAGATTAATATTCCCACTTGGTTGTCTGTCAAAGGATGAAGTTCGCAAACTTGCAACAAAGTTTGATCTGCCTAACAAAGACAGAAAGGATTCACAAGGAATATGCTTTCTAGGGAAGATCAAGTTCAATGAATTTATCGCGAGACACATAGGGGAGAAGGAAGGTGTCATATTGGAAGCTGAGTCGGGAGATTTCCTAGGAAATCATCGGGGGTTTTGGTTCTACACAATTGGTCAACGACAAGGTCTACGCCTCCCTGGAGGACCCTGGTATGTTGTTGAGAAGGATATAAAAAACAATGTAGTTTTTGTGTCAAGAAATTACTTTTCAGACAAAAAAAGAAGGCGCCTGTTTCGTGTTGGCTCCTTGAAATGGCTAAATGGTCTGCCCCCAAACCAGATCAGTCAGCTAGAGTGTAAGGTTAGGCATGGGCCTGCTTTTTGTAATTGTAGTTTAATAATGGAACCCGGTGAAGATGGTCACGAAGATGTTGCAGTTGTCCATTTATCTCAAGATGATCAAGGCCTGGCAGCTGGGCAGTTTGCGGCCTTCTACCAGGGAACAACCTGCTTGGGCTCCGGTGTAATTTTGGAGTCTTGGGATGATCAAGGTTTTCCCGTGTGTGCAAGAGCCATTGAAATTGCAAGACTGGAAGATAAATCACTTCTGGGAAAACCAGTTAAGATTAAAGTAAAGCCTGAGAATGTTATAACGGAGTCTGGTGAGGCAGATGGTACCGAACTTTGTGGAGGACTGGAAAATTCTAGAATTGCTGCTGCCAAACCACCCACGCTCATGCAGCAACTGTCGTTTATTCCGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04072 map00561 map00564 map01100 map01110 map04072

Pfam Domains

Protein Families

tRNA_Me_trans

Protein Analysis

523

Amino Acids

57.98

Weight (kDa)

6.93

Isoelectric Point (pI)

44.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_Me_trans	PF03054	84 - 287	3.7e-78	tRNA methyl transferase HUP domain
tRNA_Me_trans_M	PF20259	292 - 354	5.7e-26	tRNA methyl transferase PRC-barrel domain
tRNA_Me_trans_C	PF20258	364 - 444	8.6e-18	Aminomethyltransferase beta-barrel domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009292)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51310
fragaria_vesca	FvH4_5g33050 FvH4_5g33050 FvH4_5g33050
malus_domestica	MD03G1176400.v1.1 MD11G1196700.v1.1
prunus_persica	Prupe.5G028600_v2.0.a1 Prupe.5G028600_v2.0.a1
pyrus_communis	pycom03g13370 pycom11g16970
rosa_chinensis	RchiOBHm_Chr7g0231131
rosa_laevigata	RLG00000001432
rosa_multiflora	Rmu_co8502733.1_g000001 Rmu_sc0012403.1_g000009
rosa_roxburghii	Rroxscaffold_1G00030590 Rroxscaffold_5G00347570
rosa_rugosa	Rorug07G0264900
rosa_samantha	Rh7AG417700 Rh7BG393900 Rh7CG437700 Rh7DG411700
rosa_wichuraiana	Rw7G034590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1454
AarI	CACCTGC	1 cut(s)	652
Acc36I	ACCTGC	2 cut(s)	652, 1319
Acc65I	GGTACC	1 cut(s)	1478
AccB1I	GGYRCC	2 cut(s)	1089, 1478
AccBSI	CCGCTC	1 cut(s)	147
AccI	GTMKAC	1 cut(s)	991
AccII	CGCG	1 cut(s)	884
AciI	CCGC	8 cut(s)	29, 145, 270, 273, 298, 312, 330, 1289
AclWI	GGATC	1 cut(s)	708
AcsI	RAATTY	3 cut(s)	345, 875, 1504
AcyI	GRCGYC	1 cut(s)	1090
AfaI	GTAC	1 cut(s)	1480
AfiI	CCNNNNNNNGG	1 cut(s)	1116
AflII	CTTAAG	1 cut(s)	740
AgsI	TTSAA	5 cut(s)	199, 406, 871, 1114, 1382
AjnI	CCWGG	4 cut(s)	1000, 1010, 1269, 1299
AluBI	AGCT	5 cut(s)	195, 739, 923, 1153, 1277
AluI	AGCT	5 cut(s)	195, 739, 923, 1153, 1277
Alw26I	GTCTC	2 cut(s)	101, 880
AlwI	GGATC	1 cut(s)	708
AlwNI	CAGNNNCTG	1 cut(s)	1549
AoxI	GGCC	6 cut(s)	30, 211, 732, 1175, 1267, 1290
ApeKI	GCWGC	4 cut(s)	1274, 1517, 1520, 1543
ApoI	RAATTY	3 cut(s)	345, 875, 1504
AseI	ATTAAT	1 cut(s)	749
Asp700I	GAANNNNTTC	1 cut(s)	832
Asp718I	GGTACC	1 cut(s)	1478
AspA2I	CCTAGG	1 cut(s)	940
AspLEI	GCGC	2 cut(s)	131, 1092
AspS9I	GGNCC	4 cut(s)	212, 733, 1007, 1175
AsuC2I	CCSGG	1 cut(s)	1212
AsuHPI	GGTGA	3 cut(s)	706, 1226, 1478
AvaII	GGWCC	1 cut(s)	1007
AvrII	CCTAGG	1 cut(s)	940
BanI	GGYRCC	2 cut(s)	1089, 1478
BanII	GRGCYC	1 cut(s)	1322
BauI	CACGAG	1 cut(s)	124
BbsI	GAAGAC	1 cut(s)	366
BbvI	GCAGC	4 cut(s)	1286, 1504, 1507, 1555
BccI	CCATC	3 cut(s)	590, 1214, 1469
BceAI	ACGGC	2 cut(s)	149, 242
BcgI	CGANNNNNNTGC	2 cut(s)	1217, 1251
BciT130I	CCWGG	4 cut(s)	1002, 1012, 1271, 1301
BciVI	GTATCC	1 cut(s)	499
BclI	TGATCA	2 cut(s)	1261, 1348
BcnI	CCSGG	1 cut(s)	1212
BcoDI	GTCTC	2 cut(s)	101, 880
BfaI	CTAG	6 cut(s)	446, 674, 854, 941, 1154, 1509
BfoI	RGCGCY	1 cut(s)	1093
BfrI	CTTAAG	1 cut(s)	740
BfuAI	ACCTGC	2 cut(s)	652, 1319
BfuI	GTATCC	1 cut(s)	499
BisI	GCNGC	8 cut(s)	30, 271, 312, 1275, 1290, 1518, 1521, 1544
BlnI	CCTAGG	1 cut(s)	940
BlpI	GCTNAGC	1 cut(s)	266
BlsI	GCNGC	8 cut(s)	31, 272, 313, 1276, 1291, 1519, 1522, 1545
Bme1390I	CCNGG	5 cut(s)	1002, 1012, 1212, 1271, 1301
Bme18I	GGWCC	1 cut(s)	1007
BmgT120I	GGNCC	4 cut(s)	212, 733, 1007, 1175
BmiI	GGNNCC	7 cut(s)	353, 1009, 1091, 1108, 1209, 1321, 1480
BmrFI	CCNGG	5 cut(s)	1002, 1012, 1212, 1271, 1301
BmsI	GCATC	2 cut(s)	568, 620
BpiI	GAAGAC	1 cut(s)	366
BpmI	CTGGAG	1 cut(s)	1023
Bpu1102I	GCTNAGC	1 cut(s)	266
BpuEI	CTTGAG	2 cut(s)	56, 1239
BpuMI	CCSGG	1 cut(s)	1212
BsaHI	GRCGYC	1 cut(s)	1090
BsaJI	CCNNGG	5 cut(s)	389, 940, 1000, 1010, 1300
BsaWI	WCCGGW	1 cut(s)	1322
Bsc4I	CCNNNNNNNGG	1 cut(s)	1116
Bse118I	RCCGGY	1 cut(s)	314
Bse1I	ACTGG	4 cut(s)	17, 1398, 1421, 1503
BseBI	CCWGG	4 cut(s)	1002, 1012, 1271, 1301
BseDI	CCNNGG	5 cut(s)	389, 940, 1000, 1010, 1300
BseGI	GGATG	4 cut(s)	472, 583, 784, 1351
BseLI	CCNNNNNNNGG	1 cut(s)	1116
BseMII	CTCAG	4 cut(s)	121, 743, 915, 1434
BseNI	ACTGG	4 cut(s)	17, 1398, 1421, 1503
BseRI	GAGGAG	2 cut(s)	89, 293
BseXI	GCAGC	4 cut(s)	1286, 1504, 1507, 1555
BseYI	CCCAGC	2 cut(s)	735, 1277
BsgI	GTGCAG	1 cut(s)	310
Bsh1236I	CGCG	1 cut(s)	884
BshFI	GGCC	6 cut(s)	32, 213, 734, 1177, 1269, 1292
BshNI	GGYRCC	2 cut(s)	1089, 1478
BsiSI	CCGG	3 cut(s)	315, 1212, 1323
BslFI	GGGAC	1 cut(s)	425
BslI	CCNNNNNNNGG	1 cut(s)	1116
BsmAI	GTCTC	2 cut(s)	101, 880
BsmFI	GGGAC	1 cut(s)	425
BsmI	GAATGC	2 cut(s)	389, 569
BsnI	GGCC	6 cut(s)	32, 213, 734, 1177, 1269, 1292
Bsp1286I	GDGCHC	1 cut(s)	1322
Bsp143I	GATC	6 cut(s)	700, 808, 861, 1143, 1261, 1348
Bsp1720I	GCTNAGC	1 cut(s)	266
Bsp19I	CCATGG	1 cut(s)	389
Bsp68I	TCGCGA	1 cut(s)	884
BspACI	CCGC	8 cut(s)	29, 145, 270, 273, 298, 312, 330, 1289
BspANI	GGCC	6 cut(s)	32, 213, 734, 1177, 1269, 1292
BspCNI	CTCAG	4 cut(s)	120, 742, 916, 1435
BspFNI	CGCG	1 cut(s)	884
BspLI	GGNNCC	7 cut(s)	353, 1009, 1091, 1108, 1209, 1321, 1480
BspMI	ACCTGC	2 cut(s)	652, 1319
BspPI	GGATC	1 cut(s)	708
BspT107I	GGYRCC	2 cut(s)	1089, 1478
BspTI	CTTAAG	1 cut(s)	740
BsrBI	CCGCTC	1 cut(s)	147
BsrFI	RCCGGY	1 cut(s)	314
BsrI	ACTGG	4 cut(s)	17, 1398, 1421, 1503
BssAI	RCCGGY	1 cut(s)	314
BssECI	CCNNGG	5 cut(s)	389, 940, 1000, 1010, 1300
BssMI	GATC	6 cut(s)	700, 808, 861, 1143, 1261, 1348
BssNI	GRCGYC	1 cut(s)	1090
BssSI	CACGAG	1 cut(s)	124
BssT1I	CCWWGG	2 cut(s)	389, 940
Bst2BI	CACGAG	1 cut(s)	124
Bst2UI	CCWGG	4 cut(s)	1002, 1012, 1271, 1301
Bst4CI	ACNGT	1 cut(s)	1551
Bst6I	CTCTTC	1 cut(s)	107
BstACI	GRCGYC	1 cut(s)	1090
BstAFI	CTTAAG	1 cut(s)	740
BstC8I	GCNNGC	1 cut(s)	1179
BstDEI	CTNAG	5 cut(s)	107, 266, 729, 924, 1443
BstDSI	CCRYGG	1 cut(s)	389
BstF5I	GGATG	4 cut(s)	472, 583, 784, 1351
BstFNI	CGCG	1 cut(s)	884
BstH2I	RGCGCY	1 cut(s)	1093
BstHHI	GCGC	2 cut(s)	131, 1092
BstKTI	GATC	6 cut(s)	703, 811, 864, 1146, 1264, 1351
BstMAI	GTCTC	2 cut(s)	101, 880
BstMBI	GATC	6 cut(s)	700, 808, 861, 1143, 1261, 1348
BstMWI	GCNNNNNNNGC	4 cut(s)	29, 135, 144, 1543
BstNI	CCWGG	4 cut(s)	1002, 1012, 1271, 1301
BstSCI	CCNGG	5 cut(s)	1000, 1010, 1210, 1269, 1299
BstUI	CGCG	1 cut(s)	884
BstV1I	GCAGC	4 cut(s)	1286, 1504, 1507, 1555
BstV2I	GAAGAC	1 cut(s)	366
BsuI	GTATCC	1 cut(s)	499
BsuRI	GGCC	6 cut(s)	32, 213, 734, 1177, 1269, 1292
BtgI	CCRYGG	1 cut(s)	389
BtsCI	GGATG	4 cut(s)	472, 583, 784, 1351
BtsI	GCAGTG	2 cut(s)	154, 292
BtsIMutI	CAGTG	3 cut(s)	24, 154, 292
BtuMI	TCGCGA	1 cut(s)	884
BveI	ACCTGC	2 cut(s)	652, 1319
Cac8I	GCNNGC	1 cut(s)	1179
CaiI	CAGNNNCTG	1 cut(s)	1549
CfoI	GCGC	2 cut(s)	131, 1092
Cfr10I	RCCGGY	1 cut(s)	314
Cfr13I	GGNCC	4 cut(s)	212, 733, 1007, 1175
Csp6I	GTAC	1 cut(s)	1479
CviAII	CATG	6 cut(s)	308, 390, 574, 605, 1172, 1540
CviQI	GTAC	1 cut(s)	1479
DdeI	CTNAG	5 cut(s)	107, 266, 729, 924, 1443
DinI	GGCGCC	1 cut(s)	1091
DpnI	GATC	6 cut(s)	702, 810, 863, 1145, 1263, 1350
DpnII	GATC	6 cut(s)	700, 808, 861, 1143, 1261, 1348
Eam1104I	CTCTTC	1 cut(s)	107
EarI	CTCTTC	1 cut(s)	107
EciI	GGCGGA	1 cut(s)	287
Eco130I	CCWWGG	2 cut(s)	389, 940
Eco147I	AGGCCT	1 cut(s)	1269
Eco24I	GRGCYC	1 cut(s)	1322
Eco47I	GGWCC	1 cut(s)	1007
EcoO109I	RGGNCCY	1 cut(s)	1007
EcoRII	CCWGG	4 cut(s)	1000, 1010, 1269, 1299
EcoT14I	CCWWGG	2 cut(s)	389, 940
EcoT38I	GRGCYC	1 cut(s)	1322
EgeI	GGCGCC	1 cut(s)	1091
EheI	GGCGCC	1 cut(s)	1091
ErhI	CCWWGG	2 cut(s)	389, 940
FaeI	CATG	6 cut(s)	311, 393, 577, 608, 1175, 1543
FaqI	GGGAC	1 cut(s)	425
FatI	CATG	6 cut(s)	307, 389, 573, 604, 1171, 1539
FauI	CCCGC	1 cut(s)	152
FbaI	TGATCA	2 cut(s)	1261, 1348
FblI	GTMKAC	1 cut(s)	991
Fnu4HI	GCNGC	8 cut(s)	30, 271, 312, 1275, 1290, 1518, 1521, 1544
FokI	GGATG	4 cut(s)	479, 590, 791, 1358
FriOI	GRGCYC	1 cut(s)	1322
Fsp4HI	GCNGC	8 cut(s)	30, 271, 312, 1275, 1290, 1518, 1521, 1544
FspBI	CTAG	6 cut(s)	446, 674, 854, 941, 1154, 1509
GlaI	GCGC	2 cut(s)	130, 1091
GluI	GCNGC	8 cut(s)	30, 271, 312, 1275, 1290, 1518, 1521, 1544
GsaI	CCCAGC	2 cut(s)	739, 1281
GsuI	CTGGAG	1 cut(s)	1023
HaeII	RGCGCY	1 cut(s)	1093
HaeIII	GGCC	6 cut(s)	32, 213, 734, 1177, 1269, 1292
HapII	CCGG	3 cut(s)	315, 1212, 1323
HhaI	GCGC	2 cut(s)	131, 1092
Hin1I	GRCGYC	1 cut(s)	1090
Hin1II	CATG	6 cut(s)	311, 393, 577, 608, 1175, 1543
Hin6I	GCGC	2 cut(s)	129, 1090
HinP1I	GCGC	2 cut(s)	129, 1090
HincII	GTYRAC	1 cut(s)	980
HindII	GTYRAC	1 cut(s)	980
HinfI	GANTC	5 cut(s)	665, 833, 926, 1337, 1460
HpaII	CCGG	3 cut(s)	315, 1212, 1323
HphI	GGTGA	3 cut(s)	706, 1226, 1478
Hpy166II	GTNNAC	4 cut(s)	154, 637, 980, 992
Hpy188I	TCNGA	5 cut(s)	110, 243, 382, 705, 1075
Hpy188III	TCNNGA	7 cut(s)	674, 883, 930, 1059, 1226, 1256, 1509
Hpy8I	GTNNAC	4 cut(s)	154, 637, 980, 992
HpyAV	CCTTC	8 cut(s)	124, 343, 691, 895, 899, 1021, 1080, 1303
HpyCH4III	ACNGT	1 cut(s)	1551
HpyF10VI	GCNNNNNNNGC	4 cut(s)	29, 135, 144, 1543
HpyF3I	CTNAG	5 cut(s)	107, 266, 729, 924, 1443
Hsp92I	GRCGYC	1 cut(s)	1090
Hsp92II	CATG	6 cut(s)	311, 393, 577, 608, 1175, 1543
HspAI	GCGC	2 cut(s)	129, 1090
KasI	GGCGCC	1 cut(s)	1089
KpnI	GGTACC	1 cut(s)	1482
Ksp22I	TGATCA	2 cut(s)	1261, 1348
Kzo9I	GATC	6 cut(s)	700, 808, 861, 1143, 1261, 1348
LmnI	GCTCC	4 cut(s)	143, 152, 1112, 1325
Lsp1109I	GCAGC	4 cut(s)	1286, 1504, 1507, 1555
LweI	GCATC	2 cut(s)	568, 620
MaeI	CTAG	6 cut(s)	446, 674, 854, 941, 1154, 1509
MaeIII	GTNAC	2 cut(s)	317, 1223
MalI	GATC	6 cut(s)	702, 810, 863, 1145, 1263, 1350
MbiI	CCGCTC	1 cut(s)	147
MboI	GATC	6 cut(s)	700, 808, 861, 1143, 1261, 1348
MboII	GAAGA	9 cut(s)	94, 371, 407, 410, 490, 871, 1229, 1241, 1409
MfeI	CAATTG	1 cut(s)	972
MhlI	GDGCHC	1 cut(s)	1322
MluCI	AATT	9 cut(s)	345, 875, 972, 1063, 1189, 1329, 1383, 1504, 1512
Mly113I	GGCGCC	1 cut(s)	1090
MlyI	GAGTC	3 cut(s)	935, 1346, 1469
MroXI	GAANNNNTTC	1 cut(s)	832
MseI	TTAA	7 cut(s)	249, 501, 741, 749, 1199, 1425, 1431
MslI	CAYNNNNRTG	2 cut(s)	1230, 1538
MspA1I	CMGCKG	1 cut(s)	1277
MspCI	CTTAAG	1 cut(s)	740
MspI	CCGG	3 cut(s)	315, 1212, 1323
MspR9I	CCNGG	5 cut(s)	1002, 1012, 1212, 1271, 1301
MunI	CAATTG	1 cut(s)	972
Mva1269I	GAATGC	2 cut(s)	389, 569
MvaI	CCWGG	4 cut(s)	1002, 1012, 1271, 1301
MvnI	CGCG	1 cut(s)	884
MwoI	GCNNNNNNNGC	4 cut(s)	29, 135, 144, 1543
NarI	GGCGCC	1 cut(s)	1090
NciI	CCSGG	1 cut(s)	1212
NcoI	CCATGG	1 cut(s)	389
NdeII	GATC	6 cut(s)	700, 808, 861, 1143, 1261, 1348
NlaIII	CATG	6 cut(s)	311, 393, 577, 608, 1175, 1543
NlaIV	GGNNCC	7 cut(s)	353, 1009, 1091, 1108, 1209, 1321, 1480
NmuCI	GTSAC	2 cut(s)	317, 1223
NruI	TCGCGA	1 cut(s)	884
PaqCI	CACCTGC	1 cut(s)	652
PceI	AGGCCT	1 cut(s)	1269
PcsI	WCGNNNNNNNCGW	1 cut(s)	1559
PctI	GAATGC	2 cut(s)	389, 569
PdmI	GAANNNNTTC	1 cut(s)	832
PfeI	GAWTC	2 cut(s)	665, 833
PflFI	GACNNNGTC	1 cut(s)	987
PkrI	GCNGC	8 cut(s)	31, 272, 313, 1276, 1291, 1519, 1522, 1545
PleI	GAGTC	3 cut(s)	934, 1345, 1468
PluTI	GGCGCC	1 cut(s)	1093
PpsI	GAGTC	3 cut(s)	934, 1345, 1468
PpuMI	RGGWCCY	1 cut(s)	1007
PshBI	ATTAAT	1 cut(s)	749
PsiI	TTATAA	1 cut(s)	1454
Psp5II	RGGWCCY	1 cut(s)	1007
Psp6I	CCWGG	4 cut(s)	1000, 1010, 1269, 1299
PspFI	CCCAGC	2 cut(s)	735, 1277
PspGI	CCWGG	4 cut(s)	1000, 1010, 1269, 1299
PspN4I	GGNNCC	7 cut(s)	353, 1009, 1091, 1108, 1209, 1321, 1480
PspPI	GGNCC	4 cut(s)	212, 733, 1007, 1175
PspPPI	RGGWCCY	1 cut(s)	1007
PstNI	CAGNNNCTG	1 cut(s)	1549
PsyI	GACNNNGTC	1 cut(s)	987
PvuII	CAGCTG	1 cut(s)	1277
RruI	TCGCGA	1 cut(s)	884
RsaI	GTAC	1 cut(s)	1480
RsaNI	GTAC	1 cut(s)	1479
RseI	CAYNNNNRTG	2 cut(s)	1230, 1538
SaqAI	TTAA	7 cut(s)	249, 501, 741, 749, 1199, 1425, 1431
SatI	GCNGC	8 cut(s)	30, 271, 312, 1275, 1290, 1518, 1521, 1544
Sau3AI	GATC	6 cut(s)	700, 808, 861, 1143, 1261, 1348
Sau96I	GGNCC	4 cut(s)	212, 733, 1007, 1175
SchI	GAGTC	3 cut(s)	935, 1346, 1469
ScrFI	CCNGG	5 cut(s)	1002, 1012, 1212, 1271, 1301
SduI	GDGCHC	1 cut(s)	1322
SfaNI	GCATC	2 cut(s)	568, 620
SfoI	GGCGCC	1 cut(s)	1091
SinI	GGWCC	1 cut(s)	1007
SmiMI	CAYNNNNRTG	2 cut(s)	1230, 1538
SmlI	CTYRAG	3 cut(s)	71, 740, 1254
SmoI	CTYRAG	3 cut(s)	71, 740, 1254
Sse9I	AATT	9 cut(s)	345, 875, 972, 1063, 1189, 1329, 1383, 1504, 1512
SseBI	AGGCCT	1 cut(s)	1269
SsiI	CCGC	8 cut(s)	29, 145, 270, 273, 298, 312, 330, 1289
SspDI	GGCGCC	1 cut(s)	1089
SspI	AATATT	2 cut(s)	473, 753
SspMI	CTAG	6 cut(s)	446, 674, 854, 941, 1154, 1509
StuI	AGGCCT	1 cut(s)	1269
StyD4I	CCNGG	5 cut(s)	1000, 1010, 1210, 1269, 1299
StyI	CCWWGG	2 cut(s)	389, 940
TaaI	ACNGT	1 cut(s)	1551
TaqI	TCGA	1 cut(s)	279
TasI	AATT	9 cut(s)	345, 875, 972, 1063, 1189, 1329, 1383, 1504, 1512
TauI	GCSGC	4 cut(s)	32, 273, 314, 1292
TfiI	GAWTC	2 cut(s)	665, 833
Tru1I	TTAA	7 cut(s)	249, 501, 741, 749, 1199, 1425, 1431
Tru9I	TTAA	7 cut(s)	249, 501, 741, 749, 1199, 1425, 1431
TscAI	CASTG	3 cut(s)	24, 161, 292
TseFI	GTSAC	2 cut(s)	317, 1223
TseI	GCWGC	4 cut(s)	1274, 1517, 1520, 1543
Tsp45I	GTSAC	2 cut(s)	317, 1223
TspDTI	ATGAA	5 cut(s)	483, 562, 608, 795, 888
TspGWI	ACGGA	5 cut(s)	139, 479, 671, 1472, 1551
TspRI	CASTG	3 cut(s)	24, 161, 292
Tth111I	GACNNNGTC	1 cut(s)	987
Vha464I	CTTAAG	1 cut(s)	740
VpaK11BI	GGWCC	1 cut(s)	1007
VspI	ATTAAT	1 cut(s)	749
XapI	RAATTY	3 cut(s)	345, 875, 1504
XbaI	TCTAGA	2 cut(s)	673, 1508
XcmI	CCANNNNNNNNNTGG	1 cut(s)	611
XmaJI	CCTAGG	1 cut(s)	940
XmiI	GTMKAC	1 cut(s)	991
XmnI	GAANNNNTTC	1 cut(s)	832
XspI	CTAG	6 cut(s)	446, 674, 854, 941, 1154, 1509

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.