Rh7BG393900

tRNA-specific 2-thiouridylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0009292
Physical Location & Seq
Reverse (-)
43505849 .. 43512490
6642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG393900.1

Sequence Viewer

Length: 1176 bp
ATGCTTAGAGTGACGCCAATGGCAATGACCTCTCTACTCTCCCACATCACCTCTCTTTCTCTCTCTCTCAAACCCCCCTCCTCGTCTCTTTTCCCTCATTCTGCAAAACCCACAAAAACCCTCTTCCTCAGACCCTTCTCTATCTCCTGTTCTTCCTCCTCAGACCCTCCTCCATACAGCTTCAATGGAGATATTGGGCCTTACTTGTCCTGTTCCATGCCCCATAAGCGCCTTAAAGTTGCTGTCTTGCTTAGCGGCGGTGTCGATAGCAGTGTCGCCCTCCGTCTCCTCCACGCCGCCGGTCACTCCTGCACCGCCTTTTACCTCAAAATTTGGTTTCAAGAAGGCTTTGAGAACTTTTGGTCAGAATGCCCATGGGAAGAAGATTTGGATTATGCAAAAGCAGTCTGTAATCAGGTTGATGTCCCACTAGAAGTCGTGCATTTGACAGATGAATATTGGAAAAATGTGGTGTCCTACATTATTGAAGAGTATCGATGTGGCCGAACTCCTAACCCAGACGTTCTTTGCAATACAAGAATAAAATTTGGTGTATTCATGGATGCCATCGGCAGTATGGAGTTTGACTATGTTGCAAGTGGACATTATGCAAAAGTTGTCCACCCATCTGCAGATCAAATGGATAAGGATTCTACTTTGGAACTATCAGAAGACATGGTGAAGGATCAGACATACTTCCTTTCACATCTTTCTCAGGACCAGCTTAAGAGATTAGTATTTCCACTTGGTTGTCTGTCGAAGGATAAAGTTCGTAAACTTGCCACGAAGTTTGATCTGCCTAACAAAGAGAGAAAGGATTCACAAGGAATTTGCTTTCTTGGTAAGATAAGGTTCAGTGATTTTATTGGAAGACACATAGGGGAGAAGGAAGGTGTCATATTGGAAGCAGAGACAGGAGATCTCCTTGGAAAACATCAAGGCTTTTGGTTCTATACAATTGGTCAACGTCAAGGTCTGCGGCTCCCAGGAGGACCCTGGTACGTTGTTGAGAAGGATGTAAAAAACAATGTGGTTTTTGTGTCAAGAAATTACTTTTCAGTTGACAAAAGAAGGCGCCTGTTCCGTGTGGGATCCTTGAAATGGCTACAGGGTTTGCCCTCAAACCAGATCAGTCAGCTACAGTGCAAGGTACAGATTAAAATCCATGTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko00564 ko01100 ko01110 ko04072 map00561 map00564 map01100 map01110 map04072
Pfam Domains
Protein Families
tRNA_Me_trans

Protein Analysis

391

Amino Acids

44.32

Weight (kDa)

8.67

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_Me_trans PF03054 79 - 282 3.5e-78 tRNA methyl transferase HUP domain
tRNA_Me_trans_M PF20259 287 - 349 5e-26 tRNA methyl transferase PRC-barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1174
AccB1I GGYRCC 1 cut(s) 1074
AciI CCGC 5 cut(s) 255, 258, 297, 315, 979
AclWI GGATC 3 cut(s) 693, 1086, 1099
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 3 cut(s) 330, 545, 828
AcyI GRCGYC 2 cut(s) 14, 1075
AfaI GTAC 2 cut(s) 1001, 1152
AfiI CCNNNNNNNGG 1 cut(s) 1101
AflII CTTAAG 1 cut(s) 725
AgsI TTSAA 4 cut(s) 184, 341, 488, 1099
AjnI CCWGG 2 cut(s) 985, 995
AluBI AGCT 3 cut(s) 180, 724, 1138
AluI AGCT 3 cut(s) 180, 724, 1138
Alw26I GTCTC 3 cut(s) 90, 290, 905
AlwI GGATC 3 cut(s) 693, 1086, 1099
AoxI GGCC 2 cut(s) 197, 502
ApoI RAATTY 3 cut(s) 330, 545, 828
Asp700I GAANNNNTTC 1 cut(s) 817
AspLEI GCGC 2 cut(s) 231, 1077
AspS9I GGNCC 3 cut(s) 197, 718, 992
AsuHPI GGTGA 2 cut(s) 40, 691
AvaII GGWCC 2 cut(s) 718, 992
BamHI GGATCC 1 cut(s) 1091
BanI GGYRCC 1 cut(s) 1074
BbsI GAAGAC 2 cut(s) 678, 877
BccI CCATC 2 cut(s) 575, 634
BciT130I CCWGG 2 cut(s) 987, 997
BcoDI GTCTC 3 cut(s) 90, 290, 905
BfaI CTAG 1 cut(s) 431
BfmI CTRYAG 3 cut(s) 630, 1106, 1139
BfoI RGCGCY 2 cut(s) 232, 1078
BfrI CTTAAG 1 cut(s) 725
BglII AGATCT 1 cut(s) 919
BisI GCNGC 3 cut(s) 256, 297, 980
BlpI GCTNAGC 1 cut(s) 251
BlsI GCNGC 3 cut(s) 257, 298, 981
Bme1390I CCNGG 2 cut(s) 987, 997
Bme18I GGWCC 2 cut(s) 718, 992
BmgT120I GGNCC 3 cut(s) 197, 718, 992
BmiI GGNNCC 4 cut(s) 983, 994, 1076, 1093
BmrFI CCNGG 2 cut(s) 987, 997
BmsI GCATC 1 cut(s) 553
BpiI GAAGAC 2 cut(s) 678, 877
Bpu1102I GCTNAGC 1 cut(s) 251
Bsa29I ATCGAT 1 cut(s) 496
BsaBI GATNNNNATC 1 cut(s) 1160
BsaHI GRCGYC 2 cut(s) 14, 1075
BsaJI CCNNGG 4 cut(s) 374, 925, 985, 995
Bsc4I CCNNNNNNNGG 1 cut(s) 1101
Bse118I RCCGGY 1 cut(s) 299
Bse3DI GCAATG 1 cut(s) 30
Bse8I GATNNNNATC 1 cut(s) 1160
BseBI CCWGG 2 cut(s) 987, 997
BseCI ATCGAT 1 cut(s) 496
BseDI CCNNGG 4 cut(s) 374, 925, 985, 995
BseGI GGATG 2 cut(s) 568, 1021
BseJI GATNNNNATC 1 cut(s) 1160
BseLI CCNNNNNNNGG 1 cut(s) 1101
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 3 cut(s) 142, 174, 728
BseRI GAGGAG 4 cut(s) 70, 148, 159, 278
BsgI GTGCAG 1 cut(s) 295
BshFI GGCC 2 cut(s) 199, 504
BshNI GGYRCC 1 cut(s) 1074
BshVI ATCGAT 1 cut(s) 496
BsiSI CCGG 1 cut(s) 300
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 1 cut(s) 1101
BsmAI GTCTC 3 cut(s) 90, 290, 905
BsmBI CGTCTC 2 cut(s) 90, 290
BsmFI GGGAC 1 cut(s) 410
BsmI GAATGC 1 cut(s) 374
BsnI GGCC 2 cut(s) 199, 504
Bsp143I GATC 6 cut(s) 634, 685, 793, 919, 1091, 1128
Bsp1720I GCTNAGC 1 cut(s) 251
Bsp19I CCATGG 1 cut(s) 374
BspACI CCGC 5 cut(s) 255, 258, 297, 315, 979
BspANI GGCC 2 cut(s) 199, 504
BspCNI CTCAG 3 cut(s) 141, 173, 727
BspDI ATCGAT 1 cut(s) 496
BspLI GGNNCC 4 cut(s) 983, 994, 1076, 1093
BspMAI CTGCAG 1 cut(s) 634
BspPI GGATC 3 cut(s) 693, 1086, 1099
BspT107I GGYRCC 1 cut(s) 1074
BspTI CTTAAG 1 cut(s) 725
BsrDI GCAATG 1 cut(s) 30
BsrFI RCCGGY 1 cut(s) 299
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 4 cut(s) 374, 925, 985, 995
BssMI GATC 6 cut(s) 634, 685, 793, 919, 1091, 1128
BssNI GRCGYC 2 cut(s) 14, 1075
BssT1I CCWWGG 2 cut(s) 374, 925
Bst2UI CCWGG 2 cut(s) 987, 997
Bst4CI ACNGT 1 cut(s) 1143
Bst6I CTCTTC 2 cut(s) 128, 483
BstACI GRCGYC 2 cut(s) 14, 1075
BstAFI CTTAAG 1 cut(s) 725
BstDEI CTNAG 5 cut(s) 5, 128, 160, 251, 714
BstDSI CCRYGG 1 cut(s) 374
BstF5I GGATG 2 cut(s) 568, 1021
BstH2I RGCGCY 2 cut(s) 232, 1078
BstHHI GCGC 2 cut(s) 231, 1077
BstKTI GATC 6 cut(s) 637, 688, 796, 922, 1094, 1131
BstMAI GTCTC 3 cut(s) 90, 290, 905
BstMBI GATC 6 cut(s) 634, 685, 793, 919, 1091, 1128
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 2 cut(s) 987, 997
BstSCI CCNGG 2 cut(s) 985, 995
BstSFI CTRYAG 3 cut(s) 630, 1106, 1139
BstV2I GAAGAC 2 cut(s) 678, 877
BstX2I RGATCY 2 cut(s) 919, 1091
BstYI RGATCY 2 cut(s) 919, 1091
Bsu15I ATCGAT 1 cut(s) 496
BsuRI GGCC 2 cut(s) 199, 504
BsuTUI ATCGAT 1 cut(s) 496
BtgI CCRYGG 1 cut(s) 374
BtsCI GGATG 2 cut(s) 568, 1021
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 3 cut(s) 277, 862, 1148
CfoI GCGC 2 cut(s) 231, 1077
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 3 cut(s) 197, 718, 992
ClaI ATCGAT 1 cut(s) 496
CseI GACGC 1 cut(s) 22
Csp6I GTAC 2 cut(s) 1000, 1151
CspCI CAANNNNNGTGG 2 cut(s) 772, 807
CviAII CATG 5 cut(s) 217, 375, 559, 676, 1166
CviJI RGCY 9 cut(s) 180, 199, 348, 504, 724, 942, 982, 1105, 1138
CviKI_1 RGCY 9 cut(s) 180, 199, 348, 504, 724, 942, 982, 1105, 1138
CviQI GTAC 2 cut(s) 1000, 1151
DdeI CTNAG 5 cut(s) 5, 128, 160, 251, 714
DinI GGCGCC 1 cut(s) 1076
DpnI GATC 6 cut(s) 636, 687, 795, 921, 1093, 1130
DpnII GATC 6 cut(s) 634, 685, 793, 919, 1091, 1128
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 2 cut(s) 128, 483
EarI CTCTTC 2 cut(s) 128, 483
Eco130I CCWWGG 2 cut(s) 374, 925
Eco47I GGWCC 2 cut(s) 718, 992
EcoO109I RGGNCCY 1 cut(s) 992
EcoRII CCWGG 2 cut(s) 985, 995
EcoT14I CCWWGG 2 cut(s) 374, 925
EgeI GGCGCC 1 cut(s) 1076
EheI GGCGCC 1 cut(s) 1076
ErhI CCWWGG 2 cut(s) 374, 925
Esp3I CGTCTC 2 cut(s) 90, 290
FaeI CATG 5 cut(s) 220, 378, 562, 679, 1169
FaqI GGGAC 1 cut(s) 410
FatI CATG 5 cut(s) 216, 374, 558, 675, 1165
Fnu4HI GCNGC 3 cut(s) 256, 297, 980
FokI GGATG 2 cut(s) 575, 1028
Fsp4HI GCNGC 3 cut(s) 256, 297, 980
FspBI CTAG 1 cut(s) 431
GlaI GCGC 2 cut(s) 230, 1076
GluI GCNGC 3 cut(s) 256, 297, 980
HaeII RGCGCY 2 cut(s) 232, 1078
HaeIII GGCC 2 cut(s) 199, 504
HapII CCGG 1 cut(s) 300
HgaI GACGC 1 cut(s) 22
HhaI GCGC 2 cut(s) 231, 1077
Hin1I GRCGYC 2 cut(s) 14, 1075
Hin1II CATG 5 cut(s) 220, 378, 562, 679, 1169
Hin6I GCGC 2 cut(s) 229, 1075
HinP1I GCGC 2 cut(s) 229, 1075
HincII GTYRAC 2 cut(s) 965, 1063
HindII GTYRAC 2 cut(s) 965, 1063
HinfI GANTC 2 cut(s) 650, 818
HpaII CCGG 1 cut(s) 300
HphI GGTGA 2 cut(s) 40, 691
Hpy166II GTNNAC 5 cut(s) 602, 622, 776, 965, 1063
Hpy188I TCNGA 5 cut(s) 131, 163, 367, 670, 690
Hpy188III TCNNGA 3 cut(s) 341, 716, 1044
Hpy8I GTNNAC 5 cut(s) 602, 622, 776, 965, 1063
HpyAV CCTTC 8 cut(s) 145, 338, 676, 754, 880, 884, 1006, 1065
HpyCH4III ACNGT 1 cut(s) 1143
HpyCH4IV ACGT 3 cut(s) 522, 967, 1002
HpyCH4V TGCA 9 cut(s) 104, 312, 398, 442, 531, 596, 611, 632, 1146
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 5 cut(s) 5, 128, 160, 251, 714
HpySE526I ACGT 3 cut(s) 522, 967, 1002
Hsp92I GRCGYC 2 cut(s) 14, 1075
Hsp92II CATG 5 cut(s) 220, 378, 562, 679, 1169
HspAI GCGC 2 cut(s) 229, 1075
KasI GGCGCC 1 cut(s) 1074
Kzo9I GATC 6 cut(s) 634, 685, 793, 919, 1091, 1128
LmnI GCTCC 1 cut(s) 987
LweI GCATC 1 cut(s) 553
MaeI CTAG 1 cut(s) 431
MaeII ACGT 3 cut(s) 522, 967, 1002
MaeIII GTNAC 2 cut(s) 10, 302
MalI GATC 6 cut(s) 636, 687, 795, 921, 1093, 1130
MboI GATC 6 cut(s) 634, 685, 793, 919, 1091, 1128
MboII GAAGA 7 cut(s) 115, 144, 392, 395, 500, 683, 882
MfeI CAATTG 1 cut(s) 957
MflI RGATCY 2 cut(s) 919, 1091
MluCI AATT 5 cut(s) 330, 545, 828, 957, 1048
Mly113I GGCGCC 1 cut(s) 1075
MroXI GAANNNNTTC 1 cut(s) 817
MseI TTAA 3 cut(s) 234, 726, 1158
MspCI CTTAAG 1 cut(s) 725
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 2 cut(s) 987, 997
MunI CAATTG 1 cut(s) 957
Mva1269I GAATGC 1 cut(s) 374
MvaI CCWGG 2 cut(s) 987, 997
MwoI GCNNNNNNNGC 1 cut(s) 226
NarI GGCGCC 1 cut(s) 1075
NcoI CCATGG 1 cut(s) 374
NdeII GATC 6 cut(s) 634, 685, 793, 919, 1091, 1128
NlaIII CATG 5 cut(s) 220, 378, 562, 679, 1169
NlaIV GGNNCC 4 cut(s) 983, 994, 1076, 1093
NmuCI GTSAC 2 cut(s) 10, 302
PcsI WCGNNNNNNNCGW 1 cut(s) 502
PctI GAATGC 1 cut(s) 374
PdmI GAANNNNTTC 1 cut(s) 817
PfeI GAWTC 2 cut(s) 650, 818
PkrI GCNGC 3 cut(s) 257, 298, 981
PluTI GGCGCC 1 cut(s) 1078
PpuMI RGGWCCY 1 cut(s) 992
PsiI TTATAA 1 cut(s) 1174
Psp5II RGGWCCY 1 cut(s) 992
Psp6I CCWGG 2 cut(s) 985, 995
PspGI CCWGG 2 cut(s) 985, 995
PspN4I GGNNCC 4 cut(s) 983, 994, 1076, 1093
PspPI GGNCC 3 cut(s) 197, 718, 992
PspPPI RGGWCCY 1 cut(s) 992
PstI CTGCAG 1 cut(s) 634
PsuI RGATCY 2 cut(s) 919, 1091
RsaI GTAC 2 cut(s) 1001, 1152
RsaNI GTAC 2 cut(s) 1000, 1151
SaqAI TTAA 3 cut(s) 234, 726, 1158
SatI GCNGC 3 cut(s) 256, 297, 980
Sau3AI GATC 6 cut(s) 634, 685, 793, 919, 1091, 1128
Sau96I GGNCC 3 cut(s) 197, 718, 992
ScrFI CCNGG 2 cut(s) 987, 997
SfaNI GCATC 1 cut(s) 553
SfcI CTRYAG 3 cut(s) 630, 1106, 1139
SfoI GGCGCC 1 cut(s) 1076
SinI GGWCC 2 cut(s) 718, 992
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
Sse9I AATT 5 cut(s) 330, 545, 828, 957, 1048
SsiI CCGC 5 cut(s) 255, 258, 297, 315, 979
SspDI GGCGCC 1 cut(s) 1074
SspI AATATT 1 cut(s) 458
SspMI CTAG 1 cut(s) 431
StyD4I CCNGG 2 cut(s) 985, 995
StyI CCWWGG 2 cut(s) 374, 925
TaaI ACNGT 1 cut(s) 1143
TaiI ACGT 3 cut(s) 525, 970, 1005
TaqI TCGA 3 cut(s) 264, 496, 758
TasI AATT 5 cut(s) 330, 545, 828, 957, 1048
TauI GCSGC 3 cut(s) 258, 299, 982
TfiI GAWTC 2 cut(s) 650, 818
Tru1I TTAA 3 cut(s) 234, 726, 1158
Tru9I TTAA 3 cut(s) 234, 726, 1158
TscAI CASTG 3 cut(s) 277, 862, 1148
TseFI GTSAC 2 cut(s) 10, 302
Tsp45I GTSAC 2 cut(s) 10, 302
TspDTI ATGAA 2 cut(s) 468, 547
TspGWI ACGGA 2 cut(s) 272, 1073
TspRI CASTG 3 cut(s) 277, 862, 1148
Vha464I CTTAAG 1 cut(s) 725
VpaK11BI GGWCC 2 cut(s) 718, 992
XapI RAATTY 3 cut(s) 330, 545, 828
XcmI CCANNNNNNNNNTGG 2 cut(s) 574, 993
XmnI GAANNNNTTC 1 cut(s) 817
XspI CTAG 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.