Rmu_sc0012403.1_g000009

tRNA-specific 2-thiouridylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0012403.1
OG0009292
Physical Location & Seq
Forward (+)
40446 .. 52333
11888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0012403.1_g000009.1.cds

Sequence Viewer

Length: 1812 bp
atgcttagagtgacgccaatggcaatgacctctctactctcccacatcacctctctttctctccctctccctctcaaacccccctcctcctctcttttccctcattctgcaaaacccacaaaacccaccctcttcctcagacccttctccgtctcctgttcttcttcctcctcagaccctcctccacacagcttcaatggagatattgggccttacttgtcctgttccatgccccataagcgccttaaagttgccatcttgcttagcggcggtgtcgatagcagtgtcgccctccgtctcctccacgccgccggtcactcctgcaccgccttctacctcaaaatttggtttcaagaaggctttgagaacttttggtcagaatgcccgtgggaagaagatttggattatgcaaaagctgtctgtaatcaggttgatgtcccactagaagtcgtgcatttgacagatgaatattggaaaaatgtggtgtcctacattattgaagagtatcgatgtggccgaactcctaacccagacgttctttgcaatacaagaataaaatttggtgtattcatggatgccattggcagtatggagtttgactatgttgcaagtggacattatgcaaaagttgtccacccatctgcagatcaaatggataaggattctactttggaactatcagaagacatggtgaaggatcagacatacttcctttcacatctttctcaggaccagcttaagagattaatatttccacttggttgtctgtcgaaggataaagttcgtaaggttgccacgaagtttgatctgcctaacaaagagagaaaggattcacaaggaatttgctttcttggtaagataaggttcagtgactttattggaggacacataggggagaaggaaggtgtaatattggaagcagagacaggagatctccttggaaaacatcaaggcttttggttctatacaatcggtcaacgtcaaggtctgcggctcccaggaggaccctggtatgttgttgagaaggatgtaaaaaacaatgtggtttttgtttcaagaaattacttttcagttgacaaaagaaggcgcctgttccgtgtgggatccttgaaatggctacaggggttgccctcagaccagatcagtcagctacagtgcaaggtcagacatgggcctcggttttgcgattgtaccttaacaattgaacatggtgaagatggcaatgaagatgtggcagtcgtcaacttatctgaagatgatcaaggcctggcagcggggcaatttgcggctttctaccagggaagaacctgcttaggctccggagtaattttggaatcttgggatgatcaaggttttcctgtatgtacaaaagctcttgaaaatgctaggatggaagacaaatctcttcttgggaaaccggttaagatcaaagtaaaaccagaggctcttgtaacggagtttttcttcgaacctcgaaagagcccctcatcttttctgctcagctccaccgaagcactctctctctctcccctctttctctcgccaatggctacaataccagttaacccgaagcctttcttgaacaatctcactgggaagaccgtgttcgtgaagcttaaatggggaatggaatacaaaggtttccttgtctctgtggattcctatatgaatttgcagttggcgaacactgaagaatatattgatggacaatttactggaaatcttggggagattttgatcaggtgcaacaatgttctctatcttcgtggggtgcctgaggatgaagaaattgaagatgctgaacaggactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko00564 ko01100 ko01110 ko04072 map00561 map00564 map01100 map01110 map04072
Pfam Domains
Protein Families
tRNA_Me_trans

Protein Analysis

603

Amino Acids

67.58

Weight (kDa)

5.78

Isoelectric Point (pI)

48.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1316
AccB1I GGYRCC 2 cut(s) 1086, 1771
AccIII TCCGGA 1 cut(s) 1319
AciI CCGC 7 cut(s) 267, 270, 309, 327, 991, 1274, 1286
AclWI GGATC 3 cut(s) 705, 1098, 1111
AcoI YGGCCR 1 cut(s) 514
AcsI RAATTY 4 cut(s) 342, 557, 840, 1669
AcuI CTGAAG 2 cut(s) 1272, 1710
AcyI GRCGYC 2 cut(s) 14, 1087
AfaI GTAC 2 cut(s) 1192, 1366
AfiI CCNNNNNNNGG 2 cut(s) 1113, 1273
AflII CTTAAG 1 cut(s) 737
AgeI ACCGGT 1 cut(s) 1417
AgsI TTSAA 9 cut(s) 196, 353, 500, 1056, 1111, 1205, 1379, 1582, 1793
AjnI CCWGG 4 cut(s) 997, 1007, 1266, 1296
AjuI GAANNNNNNNTTGG 2 cut(s) 1661, 1693
AluBI AGCT 7 cut(s) 192, 416, 736, 1150, 1373, 1503, 1615
AluI AGCT 7 cut(s) 192, 416, 736, 1150, 1373, 1503, 1615
Alw26I GTCTC 4 cut(s) 157, 302, 917, 1654
AlwI GGATC 3 cut(s) 705, 1098, 1111
Aor13HI TCCGGA 1 cut(s) 1319
AoxI GGCC 4 cut(s) 209, 514, 1172, 1264
ApeKI GCWGC 1 cut(s) 1271
ApoI RAATTY 4 cut(s) 342, 557, 840, 1669
AseI ATTAAT 1 cut(s) 746
AsiGI ACCGGT 1 cut(s) 1417
Asp700I GAANNNNTTC 2 cut(s) 829, 1574
AspLEI GCGC 2 cut(s) 243, 1089
AspS9I GGNCC 4 cut(s) 209, 730, 1004, 1172
AsuHPI GGTGA 3 cut(s) 40, 703, 1223
AsuII TTCGAA 1 cut(s) 1467
AvaII GGWCC 2 cut(s) 730, 1004
AxyI CCTNAGG 1 cut(s) 1776
BamHI GGATCC 1 cut(s) 1103
BanI GGYRCC 2 cut(s) 1086, 1771
BanII GRGCYC 1 cut(s) 1484
BbsI GAAGAC 3 cut(s) 690, 1401, 1604
BbvI GCAGC 1 cut(s) 1283
BccI CCATC 5 cut(s) 263, 646, 1211, 1384, 1697
BciT130I CCWGG 4 cut(s) 999, 1009, 1268, 1298
BclI TGATCA 3 cut(s) 1258, 1345, 1737
BcoDI GTCTC 4 cut(s) 157, 302, 917, 1654
BfaI CTAG 2 cut(s) 443, 1386
BfmI CTRYAG 3 cut(s) 642, 1118, 1151
BfoI RGCGCY 2 cut(s) 244, 1090
BfrI CTTAAG 1 cut(s) 737
BfuAI ACCTGC 1 cut(s) 1316
BglII AGATCT 1 cut(s) 931
BisI GCNGC 5 cut(s) 268, 309, 992, 1272, 1287
BlpI GCTNAGC 2 cut(s) 263, 1499
BlsI GCNGC 5 cut(s) 269, 310, 993, 1273, 1288
Bme1390I CCNGG 4 cut(s) 999, 1009, 1268, 1298
Bme18I GGWCC 2 cut(s) 730, 1004
BmgT120I GGNCC 4 cut(s) 209, 730, 1004, 1172
BmiI GGNNCC 6 cut(s) 995, 1006, 1088, 1105, 1318, 1773
BmrFI CCNGG 4 cut(s) 999, 1009, 1268, 1298
BmrI ACTGGG 1 cut(s) 1602
BmsI GCATC 2 cut(s) 565, 1786
BmuI ACTGGG 1 cut(s) 1602
BpiI GAAGAC 3 cut(s) 690, 1401, 1604
Bpu10I CCTNAGC 1 cut(s) 1312
Bpu1102I GCTNAGC 2 cut(s) 263, 1499
Bpu14I TTCGAA 1 cut(s) 1467
Bsa29I ATCGAT 1 cut(s) 508
BsaBI GATNNNNATC 1 cut(s) 1736
BsaHI GRCGYC 2 cut(s) 14, 1087
BsaJI CCNNGG 6 cut(s) 386, 937, 997, 1007, 1175, 1297
BsaWI WCCGGW 2 cut(s) 1319, 1417
Bsc4I CCNNNNNNNGG 2 cut(s) 1113, 1273
Bse118I RCCGGY 2 cut(s) 311, 1417
Bse1I ACTGG 3 cut(s) 1559, 1597, 1720
Bse21I CCTNAGG 1 cut(s) 1776
Bse3DI GCAATG 2 cut(s) 30, 1228
Bse8I GATNNNNATC 1 cut(s) 1736
BseAI TCCGGA 1 cut(s) 1319
BseBI CCWGG 4 cut(s) 999, 1009, 1268, 1298
BseCI ATCGAT 1 cut(s) 508
BseDI CCNNGG 6 cut(s) 386, 937, 997, 1007, 1175, 1297
BseGI GGATG 5 cut(s) 580, 1033, 1348, 1395, 1786
BseJI GATNNNNATC 1 cut(s) 1736
BseLI CCNNNNNNNGG 2 cut(s) 1113, 1273
BseMI GCAATG 2 cut(s) 30, 1228
BseMII CTCAG 6 cut(s) 151, 186, 740, 1146, 1513, 1767
BseNI ACTGG 3 cut(s) 1559, 1597, 1720
BseRI GAGGAG 5 cut(s) 76, 79, 160, 171, 290
BseXI GCAGC 1 cut(s) 1283
BsgI GTGCAG 1 cut(s) 307
BshFI GGCC 4 cut(s) 211, 516, 1174, 1266
BshNI GGYRCC 2 cut(s) 1086, 1771
BshTI ACCGGT 1 cut(s) 1417
BshVI ATCGAT 1 cut(s) 508
BsiSI CCGG 3 cut(s) 312, 1320, 1418
BslFI GGGAC 1 cut(s) 422
BslI CCNNNNNNNGG 2 cut(s) 1113, 1273
BsmAI GTCTC 4 cut(s) 157, 302, 917, 1654
BsmBI CGTCTC 2 cut(s) 157, 302
BsmFI GGGAC 1 cut(s) 422
BsmI GAATGC 1 cut(s) 386
BsnI GGCC 4 cut(s) 211, 516, 1174, 1266
Bsp119I TTCGAA 1 cut(s) 1467
Bsp1286I GDGCHC 1 cut(s) 1484
Bsp13I TCCGGA 1 cut(s) 1319
Bsp1407I TGTACA 1 cut(s) 1364
Bsp1720I GCTNAGC 2 cut(s) 263, 1499
BspACI CCGC 7 cut(s) 267, 270, 309, 327, 991, 1274, 1286
BspANI GGCC 4 cut(s) 211, 516, 1174, 1266
BspCNI CTCAG 6 cut(s) 150, 185, 739, 1145, 1512, 1768
BspDI ATCGAT 1 cut(s) 508
BspEI TCCGGA 1 cut(s) 1319
BspLI GGNNCC 6 cut(s) 995, 1006, 1088, 1105, 1318, 1773
BspMAI CTGCAG 1 cut(s) 646
BspMI ACCTGC 1 cut(s) 1316
BspPI GGATC 3 cut(s) 705, 1098, 1111
BspT104I TTCGAA 1 cut(s) 1467
BspT107I GGYRCC 2 cut(s) 1086, 1771
BspTI CTTAAG 1 cut(s) 737
BsrDI GCAATG 2 cut(s) 30, 1228
BsrFI RCCGGY 2 cut(s) 311, 1417
BsrGI TGTACA 1 cut(s) 1364
BsrI ACTGG 3 cut(s) 1559, 1597, 1720
BssAI RCCGGY 2 cut(s) 311, 1417
BssECI CCNNGG 6 cut(s) 386, 937, 997, 1007, 1175, 1297
BssNI GRCGYC 2 cut(s) 14, 1087
BssT1I CCWWGG 1 cut(s) 937
Bst2UI CCWGG 4 cut(s) 999, 1009, 1268, 1298
Bst4CI ACNGT 2 cut(s) 1155, 1603
Bst6I CTCTTC 3 cut(s) 137, 495, 1410
BstACI GRCGYC 2 cut(s) 14, 1087
BstAFI CTTAAG 1 cut(s) 737
BstAUI TGTACA 1 cut(s) 1364
BstBI TTCGAA 1 cut(s) 1467
BstDEI CTNAG 9 cut(s) 5, 137, 172, 263, 726, 1132, 1312, 1499, 1776
BstDSI CCRYGG 1 cut(s) 386
BstF5I GGATG 5 cut(s) 580, 1033, 1348, 1395, 1786
BstH2I RGCGCY 2 cut(s) 244, 1090
BstHHI GCGC 2 cut(s) 243, 1089
BstMAI GTCTC 4 cut(s) 157, 302, 917, 1654
BstMWI GCNNNNNNNGC 1 cut(s) 238
BstNI CCWGG 4 cut(s) 999, 1009, 1268, 1298
BstSCI CCNGG 4 cut(s) 997, 1007, 1266, 1296
BstSFI CTRYAG 3 cut(s) 642, 1118, 1151
BstV1I GCAGC 1 cut(s) 1283
BstV2I GAAGAC 3 cut(s) 690, 1401, 1604
BstX2I RGATCY 2 cut(s) 931, 1103
BstYI RGATCY 2 cut(s) 931, 1103
Bsu15I ATCGAT 1 cut(s) 508
Bsu36I CCTNAGG 1 cut(s) 1776
BsuRI GGCC 4 cut(s) 211, 516, 1174, 1266
BsuTUI ATCGAT 1 cut(s) 508
BtgI CCRYGG 1 cut(s) 386
BtsCI GGATG 5 cut(s) 580, 1033, 1348, 1395, 1786
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 5 cut(s) 289, 874, 1160, 1590, 1686
BveI ACCTGC 1 cut(s) 1316
CfoI GCGC 2 cut(s) 243, 1089
Cfr10I RCCGGY 2 cut(s) 311, 1417
Cfr13I GGNCC 4 cut(s) 209, 730, 1004, 1172
ClaI ATCGAT 1 cut(s) 508
CseI GACGC 1 cut(s) 22
Csp6I GTAC 2 cut(s) 1191, 1365
CspAI ACCGGT 1 cut(s) 1417
CspCI CAANNNNNGTGG 2 cut(s) 784, 819
CviAII CATG 5 cut(s) 229, 571, 688, 1169, 1208
CviQI GTAC 2 cut(s) 1191, 1365
DdeI CTNAG 9 cut(s) 5, 137, 172, 263, 726, 1132, 1312, 1499, 1776
DinI GGCGCC 1 cut(s) 1088
EaeI YGGCCR 1 cut(s) 514
Eam1104I CTCTTC 3 cut(s) 137, 495, 1410
EarI CTCTTC 3 cut(s) 137, 495, 1410
Eco130I CCWWGG 1 cut(s) 937
Eco147I AGGCCT 1 cut(s) 1266
Eco24I GRGCYC 1 cut(s) 1484
Eco47I GGWCC 2 cut(s) 730, 1004
Eco57I CTGAAG 2 cut(s) 1272, 1710
Eco81I CCTNAGG 1 cut(s) 1776
EcoO109I RGGNCCY 1 cut(s) 1004
EcoRII CCWGG 4 cut(s) 997, 1007, 1266, 1296
EcoT14I CCWWGG 1 cut(s) 937
EcoT38I GRGCYC 1 cut(s) 1484
EgeI GGCGCC 1 cut(s) 1088
EheI GGCGCC 1 cut(s) 1088
ErhI CCWWGG 1 cut(s) 937
Esp3I CGTCTC 2 cut(s) 157, 302
FaeI CATG 5 cut(s) 232, 574, 691, 1172, 1211
FalI AAGNNNNNCTT 4 cut(s) 1562, 1594, 1629, 1661
FaqI GGGAC 1 cut(s) 422
FatI CATG 5 cut(s) 228, 570, 687, 1168, 1207
FauI CCCGC 1 cut(s) 1267
FbaI TGATCA 3 cut(s) 1258, 1345, 1737
Fnu4HI GCNGC 5 cut(s) 268, 309, 992, 1272, 1287
FokI GGATG 5 cut(s) 587, 1040, 1355, 1402, 1793
FriOI GRGCYC 1 cut(s) 1484
Fsp4HI GCNGC 5 cut(s) 268, 309, 992, 1272, 1287
FspBI CTAG 2 cut(s) 443, 1386
GlaI GCGC 2 cut(s) 242, 1088
GluI GCNGC 5 cut(s) 268, 309, 992, 1272, 1287
HaeII RGCGCY 2 cut(s) 244, 1090
HaeIII GGCC 4 cut(s) 211, 516, 1174, 1266
HapII CCGG 3 cut(s) 312, 1320, 1418
HgaI GACGC 1 cut(s) 22
HhaI GCGC 2 cut(s) 243, 1089
Hin1I GRCGYC 2 cut(s) 14, 1087
Hin1II CATG 5 cut(s) 232, 574, 691, 1172, 1211
Hin6I GCGC 2 cut(s) 241, 1087
HinP1I GCGC 2 cut(s) 241, 1087
HincII GTYRAC 4 cut(s) 977, 1075, 1243, 1564
HindII GTYRAC 4 cut(s) 977, 1075, 1243, 1564
HindIII AAGCTT 1 cut(s) 1613
HinfI GANTC 4 cut(s) 662, 830, 1334, 1658
HpaI GTTAAC 1 cut(s) 1564
HpaII CCGG 3 cut(s) 312, 1320, 1418
HphI GGTGA 3 cut(s) 40, 703, 1223
Hpy166II GTNNAC 6 cut(s) 614, 634, 977, 1075, 1243, 1564
Hpy188I TCNGA 8 cut(s) 140, 175, 379, 682, 702, 1135, 1166, 1252
Hpy188III TCNNGA 7 cut(s) 353, 728, 1056, 1320, 1376, 1579, 1609
Hpy8I GTNNAC 6 cut(s) 614, 634, 977, 1075, 1243, 1564
HpyAV CCTTC 9 cut(s) 154, 340, 350, 688, 766, 892, 896, 1018, 1077
HpyCH4III ACNGT 2 cut(s) 1155, 1603
HpyCH4IV ACGT 2 cut(s) 534, 979
HpyF10VI GCNNNNNNNGC 1 cut(s) 238
HpyF3I CTNAG 9 cut(s) 5, 137, 172, 263, 726, 1132, 1312, 1499, 1776
HpySE526I ACGT 2 cut(s) 534, 979
Hsp92I GRCGYC 2 cut(s) 14, 1087
Hsp92II CATG 5 cut(s) 232, 574, 691, 1172, 1211
HspAI GCGC 2 cut(s) 241, 1087
KasI GGCGCC 1 cut(s) 1086
Kpn2I TCCGGA 1 cut(s) 1319
Ksp22I TGATCA 3 cut(s) 1258, 1345, 1737
KspAI GTTAAC 1 cut(s) 1564
LmnI GCTCC 3 cut(s) 999, 1322, 1508
Lsp1109I GCAGC 1 cut(s) 1283
LweI GCATC 2 cut(s) 565, 1786
MaeI CTAG 2 cut(s) 443, 1386
MaeII ACGT 2 cut(s) 534, 979
MaeIII GTNAC 4 cut(s) 10, 314, 869, 1450
MfeI CAATTG 1 cut(s) 1200
MflI RGATCY 2 cut(s) 931, 1103
MhlI GDGCHC 1 cut(s) 1484
Mly113I GGCGCC 1 cut(s) 1087
MroI TCCGGA 1 cut(s) 1319
MroXI GAANNNNTTC 2 cut(s) 829, 1574
MseI TTAA 7 cut(s) 246, 738, 746, 1196, 1422, 1563, 1617
MspA1I CMGCKG 1 cut(s) 1274
MspCI CTTAAG 1 cut(s) 737
MspI CCGG 3 cut(s) 312, 1320, 1418
MspR9I CCNGG 4 cut(s) 999, 1009, 1268, 1298
MunI CAATTG 1 cut(s) 1200
Mva1269I GAATGC 1 cut(s) 386
MvaI CCWGG 4 cut(s) 999, 1009, 1268, 1298
MwoI GCNNNNNNNGC 1 cut(s) 238
NarI GGCGCC 1 cut(s) 1087
NlaIII CATG 5 cut(s) 232, 574, 691, 1172, 1211
NlaIV GGNNCC 6 cut(s) 995, 1006, 1088, 1105, 1318, 1773
NmuCI GTSAC 3 cut(s) 10, 314, 869
NspV TTCGAA 1 cut(s) 1467
PceI AGGCCT 1 cut(s) 1266
PcsI WCGNNNNNNNCGW 1 cut(s) 514
PctI GAATGC 1 cut(s) 386
PdmI GAANNNNTTC 2 cut(s) 829, 1574
PfeI GAWTC 4 cut(s) 662, 830, 1334, 1658
PinAI ACCGGT 1 cut(s) 1417
PkrI GCNGC 5 cut(s) 269, 310, 993, 1273, 1288
PluTI GGCGCC 1 cut(s) 1090
PpuMI RGGWCCY 1 cut(s) 1004
PshBI ATTAAT 1 cut(s) 746
Psp5II RGGWCCY 1 cut(s) 1004
Psp6I CCWGG 4 cut(s) 997, 1007, 1266, 1296
PspGI CCWGG 4 cut(s) 997, 1007, 1266, 1296
PspN4I GGNNCC 6 cut(s) 995, 1006, 1088, 1105, 1318, 1773
PspPI GGNCC 4 cut(s) 209, 730, 1004, 1172
PspPPI RGGWCCY 1 cut(s) 1004
PstI CTGCAG 1 cut(s) 646
PsuI RGATCY 2 cut(s) 931, 1103
RsaI GTAC 2 cut(s) 1192, 1366
RsaNI GTAC 2 cut(s) 1191, 1365
SaqAI TTAA 7 cut(s) 246, 738, 746, 1196, 1422, 1563, 1617
SatI GCNGC 5 cut(s) 268, 309, 992, 1272, 1287
Sau96I GGNCC 4 cut(s) 209, 730, 1004, 1172
ScrFI CCNGG 4 cut(s) 999, 1009, 1268, 1298
SduI GDGCHC 1 cut(s) 1484
SfaNI GCATC 2 cut(s) 565, 1786
SfcI CTRYAG 3 cut(s) 642, 1118, 1151
SfoI GGCGCC 1 cut(s) 1088
SfuI TTCGAA 1 cut(s) 1467
SinI GGWCC 2 cut(s) 730, 1004
SmlI CTYRAG 1 cut(s) 737
SmoI CTYRAG 1 cut(s) 737
SseBI AGGCCT 1 cut(s) 1266
SsiI CCGC 7 cut(s) 267, 270, 309, 327, 991, 1274, 1286
SspDI GGCGCC 1 cut(s) 1086
SspI AATATT 3 cut(s) 470, 750, 912
SspMI CTAG 2 cut(s) 443, 1386
StuI AGGCCT 1 cut(s) 1266
StyD4I CCNGG 4 cut(s) 997, 1007, 1266, 1296
StyI CCWWGG 1 cut(s) 937
TaaI ACNGT 2 cut(s) 1155, 1603
TaiI ACGT 2 cut(s) 537, 982
TaqI TCGA 5 cut(s) 276, 508, 770, 1467, 1474
TaqII GACCGA 1 cut(s) 962
TatI WGTACW 1 cut(s) 1364
TauI GCSGC 4 cut(s) 270, 311, 994, 1289
TfiI GAWTC 4 cut(s) 662, 830, 1334, 1658
Tru1I TTAA 7 cut(s) 246, 738, 746, 1196, 1422, 1563, 1617
Tru9I TTAA 7 cut(s) 246, 738, 746, 1196, 1422, 1563, 1617
TscAI CASTG 5 cut(s) 289, 874, 1160, 1597, 1693
TseFI GTSAC 3 cut(s) 10, 314, 869
TseI GCWGC 1 cut(s) 1271
Tsp45I GTSAC 3 cut(s) 10, 314, 869
TspDTI ATGAA 5 cut(s) 480, 559, 1239, 1682, 1797
TspGWI ACGGA 4 cut(s) 139, 284, 1085, 1469
TspRI CASTG 5 cut(s) 289, 874, 1160, 1597, 1693
Vha464I CTTAAG 1 cut(s) 737
VpaK11BI GGWCC 2 cut(s) 730, 1004
VspI ATTAAT 1 cut(s) 746
XapI RAATTY 4 cut(s) 342, 557, 840, 1669
XcmI CCANNNNNNNNNTGG 2 cut(s) 586, 1005
XmnI GAANNNNTTC 2 cut(s) 829, 1574
XspI CTAG 2 cut(s) 443, 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.