Rmu_co8502733.1_g000001

tRNA-specific 2-thiouridylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8502733.1
OG0009292
Physical Location & Seq
Forward (+)
1 .. 2696
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8502733.1_g000001.1.cds

Sequence Viewer

Length: 740 bp
accctcctccacacagcttcaatggagatattgggccttacttgtcctgttccatgccccataagcgccttaaagttgccatcttgcttagcggcggtgtcgatagcagtgtcgccctccgtctcctccacgccgccggtcactcctgcaccgccttctacctcaaaatttggtttcaagaaggctttgagaacttttggtcagaatgcccgtgggaagaagatttggattatgcaaaagctgtctgtaatcaggttgatgtcccactagaagtcgtgcatttgacagatgaatattggaaaaatgtggtgtcctacattattgaagagtatcgatgtggccgaactcctaacccagacgttctttgcaatacaagaataaaatttggtgtattcatggatgccattggcagtatggagtttgactatgttgcaagtggacattatgcaaaagttgtccacccatctgcagatcaaatggataaggattctactttggaactatcagaagacatggtgaaggatcagacatacttcctttcacatctttctcaggaccagcttaagagattaatatttccacttggttgtctgtcgaaggataaagttcgtaaggttgccacgaagtttgatctgcctaacaaagagagaaaggattcacaaggaatttgctttcttggtaaggtatgtcatccctgctacgactgtattcttaagggtaaatcattaatatcaacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko00564 ko01100 ko01110 ko04072 map00561 map00564 map01100 map01110 map04072
Pfam Domains
Protein Families
tRNA_Me_trans

Protein Analysis

245

Amino Acids

27.74

Weight (kDa)

5.99

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 92, 95, 134, 152
AclWI GGATC 1 cut(s) 530
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 3 cut(s) 167, 382, 665
AflII CTTAAG 2 cut(s) 562, 712
AgsI TTSAA 3 cut(s) 21, 178, 325
AluBI AGCT 3 cut(s) 17, 241, 561
AluI AGCT 3 cut(s) 17, 241, 561
Alw26I GTCTC 1 cut(s) 127
AlwI GGATC 1 cut(s) 530
AoxI GGCC 2 cut(s) 34, 339
ApoI RAATTY 3 cut(s) 167, 382, 665
AseI ATTAAT 2 cut(s) 571, 727
Asp700I GAANNNNTTC 1 cut(s) 654
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 2 cut(s) 34, 555
AsuHPI GGTGA 1 cut(s) 528
AvaII GGWCC 1 cut(s) 555
BbsI GAAGAC 1 cut(s) 515
BccI CCATC 2 cut(s) 88, 471
BcoDI GTCTC 1 cut(s) 127
BfaI CTAG 1 cut(s) 268
BfmI CTRYAG 1 cut(s) 467
BfoI RGCGCY 1 cut(s) 69
BfrI CTTAAG 2 cut(s) 562, 712
BisI GCNGC 2 cut(s) 93, 134
BlpI GCTNAGC 1 cut(s) 88
BlsI GCNGC 2 cut(s) 94, 135
Bme18I GGWCC 1 cut(s) 555
BmgT120I GGNCC 2 cut(s) 34, 555
BmsI GCATC 1 cut(s) 390
BpiI GAAGAC 1 cut(s) 515
Bpu1102I GCTNAGC 1 cut(s) 88
Bsa29I ATCGAT 1 cut(s) 333
BsaJI CCNNGG 1 cut(s) 211
Bse118I RCCGGY 1 cut(s) 136
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 211
BseGI GGATG 2 cut(s) 405, 690
BseMII CTCAG 1 cut(s) 565
BseRI GAGGAG 1 cut(s) 115
BsgI GTGCAG 1 cut(s) 132
BshFI GGCC 2 cut(s) 36, 341
BshVI ATCGAT 1 cut(s) 333
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 1 cut(s) 247
BsmAI GTCTC 1 cut(s) 127
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 247
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 2 cut(s) 36, 341
Bsp143I GATC 3 cut(s) 471, 522, 630
Bsp1720I GCTNAGC 1 cut(s) 88
BspACI CCGC 4 cut(s) 92, 95, 134, 152
BspANI GGCC 2 cut(s) 36, 341
BspCNI CTCAG 1 cut(s) 564
BspDI ATCGAT 1 cut(s) 333
BspMAI CTGCAG 1 cut(s) 471
BspPI GGATC 1 cut(s) 530
BspTI CTTAAG 2 cut(s) 562, 712
BsrFI RCCGGY 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 3 cut(s) 471, 522, 630
Bst4CI ACNGT 1 cut(s) 706
Bst6I CTCTTC 1 cut(s) 320
BstAFI CTTAAG 2 cut(s) 562, 712
BstDEI CTNAG 2 cut(s) 88, 551
BstDSI CCRYGG 1 cut(s) 211
BstF5I GGATG 2 cut(s) 405, 690
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 3 cut(s) 474, 525, 633
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 3 cut(s) 471, 522, 630
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstSFI CTRYAG 1 cut(s) 467
BstV2I GAAGAC 1 cut(s) 515
Bsu15I ATCGAT 1 cut(s) 333
BsuRI GGCC 2 cut(s) 36, 341
BsuTUI ATCGAT 1 cut(s) 333
BtgI CCRYGG 1 cut(s) 211
BtsCI GGATG 2 cut(s) 405, 690
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 114
CfoI GCGC 1 cut(s) 68
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 2 cut(s) 34, 555
ClaI ATCGAT 1 cut(s) 333
CspCI CAANNNNNGTGG 2 cut(s) 609, 644
CviAII CATG 4 cut(s) 54, 396, 513, 737
CviJI RGCY 6 cut(s) 17, 36, 185, 241, 341, 561
CviKI_1 RGCY 6 cut(s) 17, 36, 185, 241, 341, 561
DdeI CTNAG 2 cut(s) 88, 551
DpnI GATC 3 cut(s) 473, 524, 632
DpnII GATC 3 cut(s) 471, 522, 630
EaeI YGGCCR 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 320
Eco47I GGWCC 1 cut(s) 555
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 4 cut(s) 57, 399, 516, 740
FaqI GGGAC 1 cut(s) 247
FatI CATG 4 cut(s) 53, 395, 512, 736
Fnu4HI GCNGC 2 cut(s) 93, 134
FokI GGATG 2 cut(s) 412, 677
Fsp4HI GCNGC 2 cut(s) 93, 134
FspBI CTAG 1 cut(s) 268
GlaI GCGC 1 cut(s) 67
GluI GCNGC 2 cut(s) 93, 134
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 2 cut(s) 36, 341
HapII CCGG 1 cut(s) 137
HhaI GCGC 1 cut(s) 68
Hin1II CATG 4 cut(s) 57, 399, 516, 740
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HinfI GANTC 2 cut(s) 487, 655
HpaII CCGG 1 cut(s) 137
HphI GGTGA 1 cut(s) 528
Hpy166II GTNNAC 2 cut(s) 439, 459
Hpy188I TCNGA 3 cut(s) 204, 507, 527
Hpy188III TCNNGA 2 cut(s) 178, 553
Hpy8I GTNNAC 2 cut(s) 439, 459
HpyAV CCTTC 4 cut(s) 165, 175, 513, 591
HpyCH4III ACNGT 1 cut(s) 706
HpyCH4IV ACGT 1 cut(s) 359
HpyCH4V TGCA 7 cut(s) 149, 235, 279, 368, 433, 448, 469
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpyF3I CTNAG 2 cut(s) 88, 551
HpySE526I ACGT 1 cut(s) 359
Hsp92II CATG 4 cut(s) 57, 399, 516, 740
HspAI GCGC 1 cut(s) 66
Kzo9I GATC 3 cut(s) 471, 522, 630
LpnPI CCDG 8 cut(s) 60, 150, 159, 238, 368, 538, 571, 708
LweI GCATC 1 cut(s) 390
MaeI CTAG 1 cut(s) 268
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 1 cut(s) 139
MalI GATC 3 cut(s) 473, 524, 632
MboI GATC 3 cut(s) 471, 522, 630
MboII GAAGA 4 cut(s) 229, 232, 337, 520
MluCI AATT 3 cut(s) 167, 382, 665
MnlI CCTC 5 cut(s) 14, 17, 127, 136, 172
MroXI GAANNNNTTC 1 cut(s) 654
MseI TTAA 5 cut(s) 71, 563, 571, 713, 727
MspCI CTTAAG 2 cut(s) 562, 712
MspI CCGG 1 cut(s) 137
Mva1269I GAATGC 1 cut(s) 211
MwoI GCNNNNNNNGC 1 cut(s) 63
NdeII GATC 3 cut(s) 471, 522, 630
NlaIII CATG 4 cut(s) 57, 399, 516, 740
NmuCI GTSAC 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 654
PfeI GAWTC 2 cut(s) 487, 655
PkrI GCNGC 2 cut(s) 94, 135
PshBI ATTAAT 2 cut(s) 571, 727
PspPI GGNCC 2 cut(s) 34, 555
PstI CTGCAG 1 cut(s) 471
SaqAI TTAA 5 cut(s) 71, 563, 571, 713, 727
SatI GCNGC 2 cut(s) 93, 134
Sau3AI GATC 3 cut(s) 471, 522, 630
Sau96I GGNCC 2 cut(s) 34, 555
SetI ASST 8 cut(s) 19, 164, 243, 257, 362, 563, 617, 686
SfaNI GCATC 1 cut(s) 390
SfcI CTRYAG 1 cut(s) 467
SinI GGWCC 1 cut(s) 555
SmlI CTYRAG 2 cut(s) 562, 712
SmoI CTYRAG 2 cut(s) 562, 712
Sse9I AATT 3 cut(s) 167, 382, 665
SsiI CCGC 4 cut(s) 92, 95, 134, 152
SspI AATATT 2 cut(s) 295, 575
SspMI CTAG 1 cut(s) 268
TaaI ACNGT 1 cut(s) 706
TaiI ACGT 1 cut(s) 362
TaqI TCGA 3 cut(s) 101, 333, 595
TasI AATT 3 cut(s) 167, 382, 665
TauI GCSGC 2 cut(s) 95, 136
TfiI GAWTC 2 cut(s) 487, 655
Tru1I TTAA 5 cut(s) 71, 563, 571, 713, 727
Tru9I TTAA 5 cut(s) 71, 563, 571, 713, 727
TscAI CASTG 1 cut(s) 114
TseFI GTSAC 1 cut(s) 139
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 2 cut(s) 305, 384
TspGWI ACGGA 1 cut(s) 109
TspRI CASTG 1 cut(s) 114
Vha464I CTTAAG 2 cut(s) 562, 712
VpaK11BI GGWCC 1 cut(s) 555
VspI ATTAAT 2 cut(s) 571, 727
XapI RAATTY 3 cut(s) 167, 382, 665
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.