AT1G53750

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003762
Physical Location & Seq
Reverse (-)
20065394 .. 20068500
3107 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G53750.1

Sequence Viewer

Length: 1281 bp
ATGGTGAGAGATATTGAAGATGAGATTAGAGACGAGAAGAATCCTCGTCCTCTTGATGAAGATGATATCGCTCTCCTCAAGACTTATGGGTTAGGGCCTTACTCTGCACCTATCAAGAAAGTGGAGAAAGAAATTAAGGATCTCGCCAAGAAGATCAACGATCTATGTGGTATCAAGGAGTCTGATACTGGTTTAGCTCCTCCCAGTCAATGGGATCTTGTATCTGATAAGCAAATGATGCAAGAAGAGCAACCTCTGCAGGTTGCGAGATGCACAAAGATTATAAGTCCAAACACTGAAGACGCCAAATACGTCATAAATGTCAAACAAATTGCTAAGTTTGTTGTTGGACTTGGCGATAAAGTTTCTCCCACTGATATTGAGGAAGGCATGCGTGTTGGAGTTGATCGGAATAAATATCAGATTCAGATTCCTCTACCTCCCAAAATCGATCCTAGCGTGACCATGATGACTGTCGAAGAGAAACCTGATGTAACTTACAATGATGTTGGTGGATGCAAGGAGCAGATTGAGAAGATGCGAGAGGTTGTTGAACTGCCAATGCTTCATCCAGAGAAATTTGTGAAGCTTGGAATAGATCCTCCCAAGGGTGTTCTCTGCTACGGACCTCCTGGTACTGGTAAAACTCTTCTGGCTAGAGCTGTGGCCAACAGAACCGATGCTTGCTTTATCAGGGTTATTGGAAGTGAGCTTGTTCAGAAGTATGTTGGTGAAGGAGCTAGGATGGTTCGTGAACTGTTTCAGATGGCACGGTCCAAGAAAGCCTGCATTGTGTTCTTTGACGAAGTTGATGCCATTGGTGGTGCAAGATTTGATGATGGTGTTGGTGGTGATAACGAAGTCCAACGTACTATGCTTGAGATTGTGAATCAGCTTGATGGGTTTGACGCACGTGGTAACATCAAGGTTCTTATGGCAACAAACAGGCCTGACACACTAGACCCAGCTCTTTTACGTCCTGGACGTCTTGACCGTAAGGTTGAGTTTGGTCTACCTGACCTGGAGAGCAGAACACAGATCTTCAAGATTCACACAAGAACCATGAACTGCGAAAGAGACATCCGTTTCGAACTCCTTGCTCGCCTCTGCCCAAACTCAACGGGAGCTGATATTCGAAGTGTATGCACTGAAGCAGGAATGTACGCAATCAGAGCTAGGAGAAAGACTGTGACTGAGAAAGACTTTCTAGACGCAGTGAACAAAGTCATTAAAGGTTACCAAAAGTTCAGTGCCACTCCCAAGTATATGGTCTACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030162 GO:0030163 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

426

Amino Acids

47.8

Weight (kDa)

6.33

Isoelectric Point (pI)

35.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_PRS7 PF21236 79 - 147 3e-39 26S proteasome regulatory subunit 7, OB domain
AAA PF00004 205 - 338 2.4e-40 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 360 - 403 4.2e-12 AAA+ lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 284
AatII GACGTC 1 cut(s) 988
Acc36I ACCTGC 1 cut(s) 250
AccB7I CCANNNNNTGG 1 cut(s) 210
AccI GTMKAC 2 cut(s) 1012, 1272
AclWI GGATC 4 cut(s) 147, 222, 446, 593
AcoI YGGCCR 1 cut(s) 666
AcsI RAATTY 1 cut(s) 578
AcuI CTGAAG 2 cut(s) 318, 1170
AcvI CACGTG 1 cut(s) 914
AcyI GRCGYC 2 cut(s) 303, 985
AfaI GTAC 3 cut(s) 637, 871, 1163
AfiI CCNNNNNNNGG 3 cut(s) 210, 608, 638
AgsI TTSAA 3 cut(s) 17, 554, 1045
AjnI CCWGG 3 cut(s) 631, 979, 1020
AluBI AGCT 9 cut(s) 197, 589, 662, 712, 740, 895, 968, 1127, 1175
AluI AGCT 9 cut(s) 197, 589, 662, 712, 740, 895, 968, 1127, 1175
Alw26I GTCTC 2 cut(s) 24, 1071
AlwI GGATC 4 cut(s) 147, 222, 446, 593
AoxI GGCC 3 cut(s) 95, 666, 947
ApoI RAATTY 1 cut(s) 578
ArsI GACNNNNNNTTYG 2 cut(s) 1070, 1102
Asp700I GAANNNNTTC 1 cut(s) 759
AspS9I GGNCC 3 cut(s) 95, 626, 774
AsuHPI GGTGA 3 cut(s) 16, 743, 863
AsuII TTCGAA 2 cut(s) 1089, 1135
AvaII GGWCC 2 cut(s) 626, 774
BalI TGGCCA 1 cut(s) 668
BbrPI CACGTG 1 cut(s) 914
BbsI GAAGAC 1 cut(s) 306
BccI CCATC 4 cut(s) 739, 760, 833, 893
BcgI CGANNNNNNTGC 6 cut(s) 794, 828, 1079, 1113, 1125, 1159
BciT130I CCWGG 3 cut(s) 633, 981, 1022
BcoDI GTCTC 2 cut(s) 24, 1071
BfaI CTAG 6 cut(s) 456, 657, 741, 959, 1176, 1208
BfmI CTRYAG 1 cut(s) 257
BfuAI ACCTGC 1 cut(s) 250
BglII AGATCT 1 cut(s) 1038
Bme1390I CCNGG 3 cut(s) 633, 981, 1022
Bme18I GGWCC 2 cut(s) 626, 774
BmgT120I GGNCC 3 cut(s) 95, 626, 774
BmrFI CCNGG 3 cut(s) 633, 981, 1022
BmrI ACTGGG 1 cut(s) 198
BmsI GCATC 6 cut(s) 228, 260, 506, 528, 670, 802
BmuI ACTGGG 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 306
BpmI CTGGAG 1 cut(s) 1043
Bpu14I TTCGAA 2 cut(s) 1089, 1135
BpuEI CTTGAG 2 cut(s) 62, 899
Bsa29I ATCGAT 1 cut(s) 450
BsaAI YACGTR 1 cut(s) 914
BsaHI GRCGYC 2 cut(s) 303, 985
BsaJI CCNNGG 1 cut(s) 606
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 608, 638
Bse1I ACTGG 3 cut(s) 193, 204, 643
BseBI CCWGG 3 cut(s) 633, 981, 1022
BseCI ATCGAT 1 cut(s) 450
BseDI CCNNGG 1 cut(s) 606
BseGI GGATG 4 cut(s) 521, 568, 750, 1080
BseLI CCNNNNNNNGG 3 cut(s) 210, 608, 638
BseMII CTCAG 1 cut(s) 1185
BseNI ACTGG 3 cut(s) 193, 204, 643
BseRI GAGGAG 2 cut(s) 65, 189
BseYI CCCAGC 1 cut(s) 964
BsgI GTGCAG 1 cut(s) 90
BshFI GGCC 3 cut(s) 97, 668, 949
BshVI ATCGAT 1 cut(s) 450
BslI CCNNNNNNNGG 3 cut(s) 210, 608, 638
BsmAI GTCTC 2 cut(s) 24, 1071
BsmBI CGTCTC 1 cut(s) 24
BsnI GGCC 3 cut(s) 97, 668, 949
Bsp119I TTCGAA 2 cut(s) 1089, 1135
Bsp143I GATC 8 cut(s) 139, 153, 160, 214, 406, 451, 598, 1038
BspANI GGCC 3 cut(s) 97, 668, 949
BspCNI CTCAG 1 cut(s) 1186
BspDI ATCGAT 1 cut(s) 450
BspMAI CTGCAG 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 4 cut(s) 147, 222, 446, 593
BspQI GCTCTTC 1 cut(s) 240
BspT104I TTCGAA 2 cut(s) 1089, 1135
BsrI ACTGG 3 cut(s) 193, 204, 643
BssECI CCNNGG 1 cut(s) 606
BssMI GATC 8 cut(s) 139, 153, 160, 214, 406, 451, 598, 1038
BssNI GRCGYC 2 cut(s) 303, 985
BssT1I CCWWGG 1 cut(s) 606
Bst2UI CCWGG 3 cut(s) 633, 981, 1022
Bst4CI ACNGT 5 cut(s) 475, 759, 774, 995, 1189
Bst6I CTCTTC 3 cut(s) 240, 474, 654
BstACI GRCGYC 2 cut(s) 303, 985
BstAPI GCANNNNNTGC 2 cut(s) 238, 256
BstBAI YACGTR 1 cut(s) 914
BstBI TTCGAA 2 cut(s) 1089, 1135
BstC8I GCNNGC 4 cut(s) 392, 685, 787, 1102
BstDEI CTNAG 2 cut(s) 336, 1194
BstEII GGTNACC 1 cut(s) 1235
BstF5I GGATG 4 cut(s) 521, 568, 750, 1080
BstKTI GATC 8 cut(s) 142, 156, 163, 217, 409, 454, 601, 1041
BstMAI GTCTC 2 cut(s) 24, 1071
BstMBI GATC 8 cut(s) 139, 153, 160, 214, 406, 451, 598, 1038
BstMWI GCNNNNNNNGC 4 cut(s) 238, 247, 256, 1172
BstNI CCWGG 3 cut(s) 633, 981, 1022
BstNSI RCATGY 1 cut(s) 394
BstPI GGTNACC 1 cut(s) 1235
BstSCI CCNGG 3 cut(s) 631, 979, 1020
BstSFI CTRYAG 1 cut(s) 257
BstV2I GAAGAC 1 cut(s) 306
BstX2I RGATCY 4 cut(s) 139, 214, 598, 1038
BstXI CCANNNNNNTGG 1 cut(s) 1267
BstYI RGATCY 4 cut(s) 139, 214, 598, 1038
Bsu15I ATCGAT 1 cut(s) 450
BsuRI GGCC 3 cut(s) 97, 668, 949
BsuTUI ATCGAT 1 cut(s) 450
BtsCI GGATG 4 cut(s) 521, 568, 750, 1080
BtsI GCAGTG 1 cut(s) 1221
BtsIMutI CAGTG 5 cut(s) 294, 372, 1146, 1221, 1255
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 4 cut(s) 392, 685, 787, 1102
Cfr13I GGNCC 3 cut(s) 95, 626, 774
ClaI ATCGAT 1 cut(s) 450
CseI GACGC 3 cut(s) 311, 917, 1220
Csp6I GTAC 3 cut(s) 636, 870, 1162
CspCI CAANNNNNGTGG 2 cut(s) 361, 396
CviAII CATG 3 cut(s) 391, 466, 1063
CviQI GTAC 3 cut(s) 636, 870, 1162
DdeI CTNAG 2 cut(s) 336, 1194
DpnI GATC 8 cut(s) 141, 155, 162, 216, 408, 453, 600, 1040
DpnII GATC 8 cut(s) 139, 153, 160, 214, 406, 451, 598, 1038
EaeI YGGCCR 1 cut(s) 666
Eam1104I CTCTTC 3 cut(s) 240, 474, 654
EarI CTCTTC 3 cut(s) 240, 474, 654
Eco130I CCWWGG 1 cut(s) 606
Eco147I AGGCCT 1 cut(s) 949
Eco32I GATATC 1 cut(s) 67
Eco47I GGWCC 2 cut(s) 626, 774
Eco57I CTGAAG 2 cut(s) 318, 1170
Eco72I CACGTG 1 cut(s) 914
Eco91I GGTNACC 1 cut(s) 1235
EcoO109I RGGNCCY 1 cut(s) 95
EcoO65I GGTNACC 1 cut(s) 1235
EcoRII CCWGG 3 cut(s) 631, 979, 1020
EcoRV GATATC 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 606
ErhI CCWWGG 1 cut(s) 606
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 3 cut(s) 394, 469, 1066
FatI CATG 3 cut(s) 390, 465, 1062
FblI GTMKAC 2 cut(s) 1012, 1272
FokI GGATG 4 cut(s) 528, 555, 757, 1067
FspBI CTAG 6 cut(s) 456, 657, 741, 959, 1176, 1208
GsaI CCCAGC 1 cut(s) 968
GsuI CTGGAG 1 cut(s) 1043
HaeIII GGCC 3 cut(s) 97, 668, 949
HgaI GACGC 3 cut(s) 311, 917, 1220
Hin1I GRCGYC 2 cut(s) 303, 985
Hin1II CATG 3 cut(s) 394, 469, 1066
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 6 cut(s) 40, 179, 424, 430, 889, 1048
HphI GGTGA 3 cut(s) 16, 743, 863
Hpy166II GTNNAC 4 cut(s) 755, 1013, 1219, 1273
Hpy188I TCNGA 8 cut(s) 184, 226, 411, 423, 429, 720, 765, 1172
Hpy188III TCNNGA 8 cut(s) 53, 79, 115, 572, 752, 989, 1045, 1208
Hpy8I GTNNAC 4 cut(s) 755, 1013, 1219, 1273
HpyAV CCTTC 2 cut(s) 380, 728
HpyCH4III ACNGT 5 cut(s) 475, 759, 774, 995, 1189
HpyCH4IV ACGT 5 cut(s) 312, 868, 913, 976, 985
HpyCH4V TGCA 8 cut(s) 107, 241, 259, 273, 519, 789, 827, 1146
HpyF10VI GCNNNNNNNGC 4 cut(s) 238, 247, 256, 1172
HpyF3I CTNAG 2 cut(s) 336, 1194
HpySE526I ACGT 5 cut(s) 312, 868, 913, 976, 985
Hsp92I GRCGYC 2 cut(s) 303, 985
Hsp92II CATG 3 cut(s) 394, 469, 1066
Kzo9I GATC 8 cut(s) 139, 153, 160, 214, 406, 451, 598, 1038
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 4 cut(s) 202, 523, 737, 1124
LweI GCATC 6 cut(s) 228, 260, 506, 528, 670, 802
MaeI CTAG 6 cut(s) 456, 657, 741, 959, 1176, 1208
MaeII ACGT 5 cut(s) 312, 868, 913, 976, 985
MaeIII GTNAC 5 cut(s) 460, 493, 917, 1189, 1235
MalI GATC 8 cut(s) 141, 155, 162, 216, 408, 453, 600, 1040
MboI GATC 8 cut(s) 139, 153, 160, 214, 406, 451, 598, 1038
MflI RGATCY 4 cut(s) 139, 214, 598, 1038
MlsI TGGCCA 1 cut(s) 668
MluCI AATT 4 cut(s) 132, 330, 578, 1276
MluNI TGGCCA 1 cut(s) 668
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 3 cut(s) 328, 379, 889
Mox20I TGGCCA 1 cut(s) 668
MroXI GAANNNNTTC 1 cut(s) 759
MscI TGGCCA 1 cut(s) 668
MseI TTAA 2 cut(s) 135, 1230
Msp20I TGGCCA 1 cut(s) 668
MspR9I CCNGG 3 cut(s) 633, 981, 1022
MvaI CCWGG 3 cut(s) 633, 981, 1022
MwoI GCNNNNNNNGC 4 cut(s) 238, 247, 256, 1172
NdeII GATC 8 cut(s) 139, 153, 160, 214, 406, 451, 598, 1038
NlaIII CATG 3 cut(s) 394, 469, 1066
NmuCI GTSAC 2 cut(s) 460, 1189
NspI RCATGY 1 cut(s) 394
NspV TTCGAA 2 cut(s) 1089, 1135
PaeI GCATGC 1 cut(s) 394
PceI AGGCCT 1 cut(s) 949
PciSI GCTCTTC 1 cut(s) 240
PcsI WCGNNNNNNNCGW 4 cut(s) 309, 456, 982, 991
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 5 cut(s) 40, 424, 430, 889, 1048
PflMI CCANNNNNTGG 1 cut(s) 210
PfoI TCCNGGA 1 cut(s) 979
PleI GAGTC 1 cut(s) 187
PmaCI CACGTG 1 cut(s) 914
PmlI CACGTG 1 cut(s) 914
PpsI GAGTC 1 cut(s) 187
Ppu21I YACGTR 1 cut(s) 914
PsiI TTATAA 1 cut(s) 284
Psp6I CCWGG 3 cut(s) 631, 979, 1020
PspCI CACGTG 1 cut(s) 914
PspEI GGTNACC 1 cut(s) 1235
PspFI CCCAGC 1 cut(s) 964
PspGI CCWGG 3 cut(s) 631, 979, 1020
PspPI GGNCC 3 cut(s) 95, 626, 774
PstI CTGCAG 1 cut(s) 261
PsuI RGATCY 4 cut(s) 139, 214, 598, 1038
RsaI GTAC 3 cut(s) 637, 871, 1163
RsaNI GTAC 3 cut(s) 636, 870, 1162
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 2 cut(s) 135, 1230
Sau3AI GATC 8 cut(s) 139, 153, 160, 214, 406, 451, 598, 1038
Sau96I GGNCC 3 cut(s) 95, 626, 774
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 3 cut(s) 633, 981, 1022
SfaNI GCATC 6 cut(s) 228, 260, 506, 528, 670, 802
SfcI CTRYAG 1 cut(s) 257
SfuI TTCGAA 2 cut(s) 1089, 1135
SinI GGWCC 2 cut(s) 626, 774
SmlI CTYRAG 2 cut(s) 77, 878
SmoI CTYRAG 2 cut(s) 77, 878
SphI GCATGC 1 cut(s) 394
Sse9I AATT 4 cut(s) 132, 330, 578, 1276
SseBI AGGCCT 1 cut(s) 949
SspMI CTAG 6 cut(s) 456, 657, 741, 959, 1176, 1208
StuI AGGCCT 1 cut(s) 949
StyD4I CCNGG 3 cut(s) 631, 979, 1020
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 5 cut(s) 475, 759, 774, 995, 1189
TaiI ACGT 5 cut(s) 315, 871, 916, 979, 988
TaqI TCGA 4 cut(s) 450, 477, 1089, 1135
TasI AATT 4 cut(s) 132, 330, 578, 1276
TfiI GAWTC 5 cut(s) 40, 424, 430, 889, 1048
Tru1I TTAA 2 cut(s) 135, 1230
Tru9I TTAA 2 cut(s) 135, 1230
TscAI CASTG 5 cut(s) 301, 379, 1153, 1221, 1255
TseFI GTSAC 2 cut(s) 460, 1189
Tsp45I GTSAC 2 cut(s) 460, 1189
TspDTI ATGAA 3 cut(s) 72, 557, 1079
TspGWI ACGGA 2 cut(s) 639, 1073
TspRI CASTG 5 cut(s) 301, 379, 1153, 1221, 1255
Van91I CCANNNNNTGG 1 cut(s) 210
VpaK11BI GGWCC 2 cut(s) 626, 774
XapI RAATTY 1 cut(s) 578
XbaI TCTAGA 1 cut(s) 1207
XceI RCATGY 1 cut(s) 394
XmiI GTMKAC 2 cut(s) 1012, 1272
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 6 cut(s) 456, 657, 741, 959, 1176, 1208
ZraI GACGTC 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.