Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006488

Physical Location & Seq

Genomic Coordinates Reverse (-)

20336203 .. 20339769

Gene Structure 3567 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G54450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1608 bp

ATGGAGTCGATAACGTTAGATATAGAGCTGTTGCAGTTACCAGAGACATCGCCAATGTCGATGAAATCTAATCAAGATTTTGTTAAGAAGCTCTTTGATCAATGGCTTGCTCTTCCTGAAACCAATCGTTTGGTTACATCTTTGGTAAATGATGCAAAGGCAGGAGTGGCTTTGAATGTTATGTGTGGAGGAGGCTCTTCTGGCACGAATTCAGGGTCGAATAGTCCATTAGCTTCAATGTTTCCGGCACGCAATGGGCCTCCTCTTTCTCCAAGGAATTCAACTGGTTCTCCTCGGATCGCCAGACAAAGAACTGGACTATCAAATCTTAGTTCTCCTCTCAAAGTAGTTTCTGATCATGTTAAAGAACTCATTCCTCAGTTTTACTTTGAAGATGGTCGGCCACCACCAAATGATCTAAAAGAGCAGTGCATAGCTAAGATCAATAGTCTTTTCTATGGCCACGAGGATGGTTTGCAACTCCAAGAATTTAAATTAGTCACTACTGAAATATGCAAAGTTCCATCATTTTTTTCCACCTCCATTTTCAAGAAAGTTGATACCAATAACACTGGTTTTGTGAAAAGAGAAGACTTCATTGATTATTGGGTTAAGGGAAATATGTTAACAAAGGAGATAACAAGTCAAGTATTTACAATATTGAAGCAGCCAGATCACAACTACCTTGTCCAGGATGATTTCAAGCCAGTGCTTCAAGAATTATTGGCAACACACCCTGGTCTTGAATTCTTGCAAGGCACACCGGAATTTCAGGATAGATATGCTGAAACTGTAATATACAGAATATATTACTACATAAATAGGAGTGGAAATGGGCATCTTACCTTGAGAGAGCTTAAGCGAGGAAATTTAGTTGACGCAATGCAGCATGCCGATGAAGAAGAGGACATCAATAAGGTGTTGAGGTACTTCTCGTATGAACATTTCTATGTCATATACTGCAAGTTCTGGGAGTTGGATACAGATCATGATTTCTTAATCGACAAAGAGAATCTCATCCGATATAGTAACCATGCGCTCACCTATAGGATTGTCGATCGAATCTTTTCACAGGTACCGAGGAAGTTTACTAGTAAAACCGAAGGAAAGATGGGTTATGAGGATTTTGTTTACTTCATATTGGCTGAAGAAGACAAGTCATCAGAACCTAGTCTTGAGTATTGGTTCAAGTGCATTGATTTGGATGCAAATGGAGTTTTGACGCGGAACGAGCTGCAATTCTTTTACGAGGAGCAGCTACATAGAATGGAATGCATGGCGCAAGAGGCGGTGTTGTTTGAGGATATTTTGTGCCAATTATTCGACATGGTCAAACCAGAGGACGAAGGGTTCATTTGTCTAAATGATTTGAAGGGTTCTAAACTTTCTGGAAATGTCTTCAACATACTTTTTAACCTCAACAAGTTTATGGCGTTTGAAACGCGTGATCCCTTCCTCATCCGCCAGGAGCGCGCAAATCCGACATGGACAGAGTGGGATCGTTTTGCTCATAGAGAATATATTAGGCTATCAATGGAAGAAGATGTCGAAGATGCTTCTAATGGAAGTGCTGAAGCATGGGACGACTCACTTGAGGTCCCCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03015 ko04071 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04151 map04152 map04261 map04728 map05165

Pfam Domains

Protein Families

EF-hand_5 EF-hand_7

Protein Analysis

535

Amino Acids

61.94

Weight (kDa)

4.89

Isoelectric Point (pI)

40.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_13	PF17958	215 - 301	1.3e-32	EF-hand domain
EF-hand_7	PF13499	317 - 416	6.2e-10	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54450 AT5G28850 AT5G28850 AT5G28900
fragaria_vesca	FvH4_6g01930
malus_domestica	MD04G1230600.v1.1 MD12G1249000.v1.1
prunus_persica	Prupe.6G350400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0449671 RchiOBHm_Chr3g0449721
rosa_laevigata	RLG00000025807 RLG00000025809
rosa_multiflora	Rmu_sc0009629.1_g000003 Rmu_sc0009629.1_g000010 Rmu_sc0010999.1_g000003
rosa_roxburghii	Rroxscaffold_6G00427570 Rroxscaffold_6G00427590
rosa_rugosa	Rorug02G0622700
rosa_samantha	Rh3AG020800 Rh3AG021100 Rh3BG021500 Rh3BG021800 Rh3CG020000 Rh3DG021300 Rh3DG021600
rosa_wichuraiana	Rw3G001720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1075
AccB1I	GGYRCC	1 cut(s)	1075
AccII	CGCG	3 cut(s)	1225, 1444, 1473
AciI	CCGC	3 cut(s)	1225, 1289, 1462
AclI	AACGTT	1 cut(s)	14
AclWI	GGATC	3 cut(s)	305, 1442, 1506
AcoI	YGGCCR	2 cut(s)	401, 460
AcsI	RAATTY	6 cut(s)	208, 277, 488, 746, 767, 868
AcuI	CTGAAG	2 cut(s)	1167, 1593
AfaI	GTAC	2 cut(s)	929, 1077
AfiI	CCNNNNNNNGG	1 cut(s)	691
AflII	CTTAAG	1 cut(s)	857
AflIII	ACRYGT	1 cut(s)	1442
AhlI	ACTAGT	1 cut(s)	1091
AjnI	CCWGG	3 cut(s)	690, 736, 1464
AloI	GAACNNNNNNTCC	2 cut(s)	1334, 1366
AluBI	AGCT	7 cut(s)	28, 91, 233, 437, 856, 1234, 1258
AluI	AGCT	7 cut(s)	28, 91, 233, 437, 856, 1234, 1258
Alw26I	GTCTC	1 cut(s)	38
AlwI	GGATC	3 cut(s)	305, 1442, 1506
AoxI	GGCC	3 cut(s)	257, 401, 460
ApeKI	GCWGC	4 cut(s)	667, 886, 1234, 1255
ApoI	RAATTY	6 cut(s)	208, 277, 488, 746, 767, 868
ArsI	GACNNNNNNTTYG	2 cut(s)	200, 232
Asp700I	GAANNNNTTC	2 cut(s)	372, 1066
Asp718I	GGTACC	1 cut(s)	1075
AspLEI	GCGC	4 cut(s)	1039, 1282, 1473, 1475
AspS9I	GGNCC	2 cut(s)	257, 1597
AsuHPI	GGTGA	1 cut(s)	1033
AvaII	GGWCC	1 cut(s)	1597
BalI	TGGCCA	1 cut(s)	462
BanI	GGYRCC	1 cut(s)	1075
BauI	CACGAG	1 cut(s)	464
BbsI	GAAGAC	3 cut(s)	597, 1158, 1390
BbvI	GCAGC	4 cut(s)	679, 898, 1221, 1267
BccI	CCATC	4 cut(s)	389, 464, 532, 1105
BciT130I	CCWGG	3 cut(s)	692, 738, 1466
BciVI	GTATCC	1 cut(s)	973
BclI	TGATCA	2 cut(s)	97, 355
BcoDI	GTCTC	1 cut(s)	38
BcuI	ACTAGT	1 cut(s)	1091
BfaI	CTAG	2 cut(s)	1092, 1170
BfmI	CTRYAG	1 cut(s)	1045
BfrI	CTTAAG	1 cut(s)	857
BfuI	GTATCC	1 cut(s)	973
BisI	GCNGC	4 cut(s)	668, 887, 1235, 1256
BlsI	GCNGC	4 cut(s)	669, 888, 1236, 1257
Bme1390I	CCNGG	3 cut(s)	692, 738, 1466
Bme18I	GGWCC	1 cut(s)	1597
BmgT120I	GGNCC	2 cut(s)	257, 1597
BmiI	GGNNCC	2 cut(s)	1077, 1599
BmrFI	CCNGG	3 cut(s)	692, 738, 1466
BmsI	GCATC	4 cut(s)	142, 847, 1195, 1543
BpiI	GAAGAC	3 cut(s)	597, 1158, 1390
BpuEI	CTTGAG	2 cut(s)	868, 1196
BsaBI	GATNNNNATC	1 cut(s)	984
BsaJI	CCNNGG	4 cut(s)	272, 293, 736, 1079
BsaWI	WCCGGW	1 cut(s)	763
BsaXI	ACNNNNNCTCC	2 cut(s)	274, 304
Bsc4I	CCNNNNNNNGG	1 cut(s)	691
Bse1I	ACTGG	4 cut(s)	289, 319, 577, 707
Bse3DI	GCAATG	2 cut(s)	259, 888
Bse8I	GATNNNNATC	1 cut(s)	984
BseBI	CCWGG	3 cut(s)	692, 738, 1466
BseDI	CCNNGG	4 cut(s)	272, 293, 736, 1079
BseGI	GGATG	5 cut(s)	475, 700, 1017, 1210, 1458
BseJI	GATNNNNATC	1 cut(s)	984
BseLI	CCNNNNNNNGG	1 cut(s)	691
BseMI	GCAATG	2 cut(s)	259, 888
BseMII	CTCAG	1 cut(s)	392
BseNI	ACTGG	4 cut(s)	289, 319, 577, 707
BsePI	GCGCGC	1 cut(s)	1471
BseRI	GAGGAG	5 cut(s)	204, 252, 282, 327, 1265
BseXI	GCAGC	4 cut(s)	679, 898, 1221, 1267
Bsh1236I	CGCG	3 cut(s)	1225, 1444, 1473
Bsh1285I	CGRYCG	1 cut(s)	1060
BshFI	GGCC	3 cut(s)	259, 403, 462
BshNI	GGYRCC	1 cut(s)	1075
BsiEI	CGRYCG	1 cut(s)	1060
BsiSI	CCGG	2 cut(s)	245, 764
BslFI	GGGAC	2 cut(s)	1583, 1595
BslI	CCNNNNNNNGG	1 cut(s)	691
BsmAI	GTCTC	1 cut(s)	38
BsmFI	GGGAC	2 cut(s)	1583, 1595
BsmI	GAATGC	1 cut(s)	1277
BsnI	GGCC	3 cut(s)	259, 403, 462
BspACI	CCGC	3 cut(s)	1225, 1289, 1462
BspANI	GGCC	3 cut(s)	259, 403, 462
BspCNI	CTCAG	1 cut(s)	391
BspFNI	CGCG	3 cut(s)	1225, 1444, 1473
BspHI	TCATGA	1 cut(s)	988
BspLI	GGNNCC	2 cut(s)	1077, 1599
BspPI	GGATC	3 cut(s)	305, 1442, 1506
BspQI	GCTCTTC	2 cut(s)	117, 202
BspT107I	GGYRCC	1 cut(s)	1075
BspTI	CTTAAG	1 cut(s)	857
BsrDI	GCAATG	2 cut(s)	259, 888
BsrI	ACTGG	4 cut(s)	289, 319, 577, 707
BssECI	CCNNGG	4 cut(s)	272, 293, 736, 1079
BssHII	GCGCGC	1 cut(s)	1471
BssSI	CACGAG	1 cut(s)	464
BssT1I	CCWWGG	1 cut(s)	272
Bst2BI	CACGAG	1 cut(s)	464
Bst2UI	CCWGG	3 cut(s)	692, 738, 1466
Bst4CI	ACNGT	1 cut(s)	793
Bst6I	CTCTTC	3 cut(s)	117, 202, 897
BstAFI	CTTAAG	1 cut(s)	857
BstC8I	GCNNGC	4 cut(s)	108, 250, 891, 1473
BstDEI	CTNAG	3 cut(s)	329, 378, 438
BstENI	CCTNNNNNAGG	1 cut(s)	689
BstF5I	GGATG	5 cut(s)	475, 700, 1017, 1210, 1458
BstFNI	CGCG	3 cut(s)	1225, 1444, 1473
BstHHI	GCGC	4 cut(s)	1039, 1282, 1473, 1475
BstMAI	GTCTC	1 cut(s)	38
BstMCI	CGRYCG	1 cut(s)	1060
BstMWI	GCNNNNNNNGC	5 cut(s)	167, 201, 1231, 1286, 1470
BstNI	CCWGG	3 cut(s)	692, 738, 1466
BstNSI	RCATGY	1 cut(s)	893
BstSCI	CCNGG	3 cut(s)	690, 736, 1464
BstSFI	CTRYAG	1 cut(s)	1045
BstUI	CGCG	3 cut(s)	1225, 1444, 1473
BstV1I	GCAGC	4 cut(s)	679, 898, 1221, 1267
BstV2I	GAAGAC	3 cut(s)	597, 1158, 1390
BstXI	CCANNNNNNTGG	2 cut(s)	130, 470
BsuI	GTATCC	1 cut(s)	973
BsuRI	GGCC	3 cut(s)	259, 403, 462
BtgZI	GCGATG	1 cut(s)	33
BtsCI	GGATG	5 cut(s)	475, 700, 1017, 1210, 1458
BtsI	GCAGTG	1 cut(s)	434
BtsIMutI	CAGTG	3 cut(s)	434, 570, 714
Cac8I	GCNNGC	4 cut(s)	108, 250, 891, 1473
CciI	TCATGA	1 cut(s)	988
CfoI	GCGC	4 cut(s)	1039, 1282, 1473, 1475
Cfr13I	GGNCC	2 cut(s)	257, 1597
CseI	GACGC	2 cut(s)	887, 1231
Csp6I	GTAC	2 cut(s)	928, 1076
CviAII	CATG	8 cut(s)	359, 890, 989, 1034, 1276, 1327, 1485, 1578
CviQI	GTAC	2 cut(s)	928, 1076
DdeI	CTNAG	3 cut(s)	329, 378, 438
DraI	TTTAAA	1 cut(s)	493
EaeI	YGGCCR	2 cut(s)	401, 460
Eam1104I	CTCTTC	3 cut(s)	117, 202, 897
EarI	CTCTTC	3 cut(s)	117, 202, 897
EciI	GGCGGA	1 cut(s)	1451
Eco130I	CCWWGG	1 cut(s)	272
Eco47I	GGWCC	1 cut(s)	1597
Eco57I	CTGAAG	2 cut(s)	1167, 1593
EcoNI	CCTNNNNNAGG	1 cut(s)	689
EcoO109I	RGGNCCY	1 cut(s)	1597
EcoRI	GAATTC	3 cut(s)	208, 277, 746
EcoRII	CCWGG	3 cut(s)	690, 736, 1464
EcoT14I	CCWWGG	1 cut(s)	272
EcoT22I	ATGCAT	1 cut(s)	1277
ErhI	CCWWGG	1 cut(s)	272
FaeI	CATG	8 cut(s)	362, 893, 992, 1037, 1279, 1330, 1488, 1581
FalI	AAGNNNNNCTT	2 cut(s)	77, 109
FaqI	GGGAC	2 cut(s)	1583, 1595
FatI	CATG	8 cut(s)	358, 889, 988, 1033, 1275, 1326, 1484, 1577
FbaI	TGATCA	2 cut(s)	97, 355
Fnu4HI	GCNGC	4 cut(s)	668, 887, 1235, 1256
FokI	GGATG	5 cut(s)	482, 707, 1004, 1217, 1445
Fsp4HI	GCNGC	4 cut(s)	668, 887, 1235, 1256
FspBI	CTAG	2 cut(s)	1092, 1170
GlaI	GCGC	4 cut(s)	1038, 1281, 1472, 1474
GluI	GCNGC	4 cut(s)	668, 887, 1235, 1256
HaeIII	GGCC	3 cut(s)	259, 403, 462
HapII	CCGG	2 cut(s)	245, 764
HgaI	GACGC	2 cut(s)	887, 1231
HhaI	GCGC	4 cut(s)	1039, 1282, 1473, 1475
Hin1II	CATG	8 cut(s)	362, 893, 992, 1037, 1279, 1330, 1488, 1581
Hin6I	GCGC	4 cut(s)	1037, 1280, 1471, 1473
HinP1I	GCGC	4 cut(s)	1037, 1280, 1471, 1473
HincII	GTYRAC	2 cut(s)	627, 877
HindII	GTYRAC	2 cut(s)	627, 877
HinfI	GANTC	4 cut(s)	5, 1012, 1062, 1586
HpaI	GTTAAC	1 cut(s)	627
HpaII	CCGG	2 cut(s)	245, 764
HphI	GGTGA	1 cut(s)	1033
Hpy166II	GTNNAC	4 cut(s)	627, 877, 1089, 1132
Hpy188I	TCNGA	5 cut(s)	297, 355, 1022, 1165, 1482
Hpy188III	TCNNGA	9 cut(s)	74, 116, 550, 716, 743, 773, 989, 1175, 1389
Hpy8I	GTNNAC	4 cut(s)	627, 877, 1089, 1132
HpyAV	CCTTC	4 cut(s)	1097, 1340, 1366, 1462
HpyCH4III	ACNGT	1 cut(s)	793
HpyCH4IV	ACGT	1 cut(s)	14
HpyF10VI	GCNNNNNNNGC	5 cut(s)	167, 201, 1231, 1286, 1470
HpyF3I	CTNAG	3 cut(s)	329, 378, 438
HpySE526I	ACGT	1 cut(s)	14
Hsp92II	CATG	8 cut(s)	362, 893, 992, 1037, 1279, 1330, 1488, 1581
HspAI	GCGC	4 cut(s)	1037, 1280, 1471, 1473
KpnI	GGTACC	1 cut(s)	1079
Ksp22I	TGATCA	2 cut(s)	97, 355
KspAI	GTTAAC	1 cut(s)	627
LguI	GCTCTTC	2 cut(s)	117, 202
LmnI	GCTCC	2 cut(s)	1252, 1468
Lsp1109I	GCAGC	4 cut(s)	679, 898, 1221, 1267
LweI	GCATC	4 cut(s)	142, 847, 1195, 1543
MaeI	CTAG	2 cut(s)	1092, 1170
MaeII	ACGT	1 cut(s)	14
MaeIII	GTNAC	4 cut(s)	36, 133, 499, 1028
MlsI	TGGCCA	1 cut(s)	462
MluI	ACGCGT	1 cut(s)	1442
MluNI	TGGCCA	1 cut(s)	462
MlyI	GAGTC	2 cut(s)	14, 1580
MmeI	TCCRAC	2 cut(s)	957, 1505
Mox20I	TGGCCA	1 cut(s)	462
Mph1103I	ATGCAT	1 cut(s)	1277
MroXI	GAANNNNTTC	2 cut(s)	372, 1066
MscI	TGGCCA	1 cut(s)	462
MseI	TTAA	8 cut(s)	84, 363, 492, 612, 626, 858, 998, 1413
MslI	CAYNNNNRTG	3 cut(s)	468, 894, 948
Msp20I	TGGCCA	1 cut(s)	462
MspCI	CTTAAG	1 cut(s)	857
MspI	CCGG	2 cut(s)	245, 764
MspR9I	CCNGG	3 cut(s)	692, 738, 1466
Mva1269I	GAATGC	1 cut(s)	1277
MvaI	CCWGG	3 cut(s)	692, 738, 1466
MvnI	CGCG	3 cut(s)	1225, 1444, 1473
MwoI	GCNNNNNNNGC	5 cut(s)	167, 201, 1231, 1286, 1470
NlaIII	CATG	8 cut(s)	362, 893, 992, 1037, 1279, 1330, 1488, 1581
NlaIV	GGNNCC	2 cut(s)	1077, 1599
NmuCI	GTSAC	1 cut(s)	499
NsiI	ATGCAT	1 cut(s)	1277
NspI	RCATGY	1 cut(s)	893
PaeI	GCATGC	1 cut(s)	893
PagI	TCATGA	1 cut(s)	988
PauI	GCGCGC	1 cut(s)	1471
PciSI	GCTCTTC	2 cut(s)	117, 202
PcsI	WCGNNNNNNNCGW	1 cut(s)	56
PctI	GAATGC	1 cut(s)	1277
PdmI	GAANNNNTTC	2 cut(s)	372, 1066
PfeI	GAWTC	2 cut(s)	1012, 1062
PflFI	GACNNNGTC	1 cut(s)	1328
PfoI	TCCNGGA	1 cut(s)	690
PkrI	GCNGC	4 cut(s)	669, 888, 1236, 1257
Ple19I	CGATCG	1 cut(s)	1060
PleI	GAGTC	2 cut(s)	13, 1580
PpsI	GAGTC	2 cut(s)	13, 1580
PpuMI	RGGWCCY	1 cut(s)	1597
Psp1406I	AACGTT	1 cut(s)	14
Psp5II	RGGWCCY	1 cut(s)	1597
Psp6I	CCWGG	3 cut(s)	690, 736, 1464
PspGI	CCWGG	3 cut(s)	690, 736, 1464
PspN4I	GGNNCC	2 cut(s)	1077, 1599
PspPI	GGNCC	2 cut(s)	257, 1597
PspPPI	RGGWCCY	1 cut(s)	1597
PsyI	GACNNNGTC	1 cut(s)	1328
PteI	GCGCGC	1 cut(s)	1471
PvuI	CGATCG	1 cut(s)	1060
RsaI	GTAC	2 cut(s)	929, 1077
RsaNI	GTAC	2 cut(s)	928, 1076
RseI	CAYNNNNRTG	3 cut(s)	468, 894, 948
SapI	GCTCTTC	2 cut(s)	117, 202
SaqAI	TTAA	8 cut(s)	84, 363, 492, 612, 626, 858, 998, 1413
SatI	GCNGC	4 cut(s)	668, 887, 1235, 1256
Sau96I	GGNCC	2 cut(s)	257, 1597
SchI	GAGTC	2 cut(s)	14, 1580
ScrFI	CCNGG	3 cut(s)	692, 738, 1466
SfaNI	GCATC	4 cut(s)	142, 847, 1195, 1543
SfcI	CTRYAG	1 cut(s)	1045
SinI	GGWCC	1 cut(s)	1597
SmiI	ATTTAAAT	1 cut(s)	493
SmiMI	CAYNNNNRTG	3 cut(s)	468, 894, 948
SmlI	CTYRAG	4 cut(s)	847, 857, 1175, 1592
SmoI	CTYRAG	4 cut(s)	847, 857, 1175, 1592
SpeI	ACTAGT	1 cut(s)	1091
SphI	GCATGC	1 cut(s)	893
SsiI	CCGC	3 cut(s)	1225, 1289, 1462
SspI	AATATT	1 cut(s)	660
SspMI	CTAG	2 cut(s)	1092, 1170
StyD4I	CCNGG	3 cut(s)	690, 736, 1464
StyI	CCWWGG	1 cut(s)	272
SwaI	ATTTAAAT	1 cut(s)	493
TaaI	ACNGT	1 cut(s)	793
TaiI	ACGT	1 cut(s)	17
TaqI	TCGA	8 cut(s)	8, 59, 218, 1002, 1056, 1060, 1323, 1548
TfiI	GAWTC	2 cut(s)	1012, 1062
Tru1I	TTAA	8 cut(s)	84, 363, 492, 612, 626, 858, 998, 1413
Tru9I	TTAA	8 cut(s)	84, 363, 492, 612, 626, 858, 998, 1413
TscAI	CASTG	3 cut(s)	434, 577, 714
TseFI	GTSAC	1 cut(s)	499
TseI	GCWGC	4 cut(s)	667, 886, 1234, 1255
Tsp45I	GTSAC	1 cut(s)	499
TspDTI	ATGAA	6 cut(s)	77, 586, 912, 954, 1126, 1342
TspRI	CASTG	3 cut(s)	434, 577, 714
Tth111I	GACNNNGTC	1 cut(s)	1328
Vha464I	CTTAAG	1 cut(s)	857
VpaK11BI	GGWCC	1 cut(s)	1597
XagI	CCTNNNNNAGG	1 cut(s)	689
XapI	RAATTY	6 cut(s)	208, 277, 488, 746, 767, 868
XceI	RCATGY	1 cut(s)	893
XmnI	GAANNNNTTC	2 cut(s)	372, 1066
XspI	CTAG	2 cut(s)	1092, 1170
Zsp2I	ATGCAT	1 cut(s)	1277

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.