Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006488

Physical Location & Seq

Genomic Coordinates Forward (+)

31049265 .. 31054140

Gene Structure 4876 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1230600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1308 bp

ATGAAACAGAGGATTGGTCCTTCTCATTTAGGTTCTCCCCTGAAAGTAGTTAGCGAGCCGGTTAAGGAAGTGATACCTCAGTTTTACTTTCTACATGGTCGCCCACCTCCAAATGAACTAAAAGATCAATGCTTGTTTAGAATCAATCAGTTTTTCTCTGGCAACTCAGATGGACTGCAGATGCATGAATTTAAATCAGTTGCTAAGGATATCTGCAAGCTGCCTTCATTCTTCTCCACATCTCTCTTCAAAAAGATTGATGCTAATAGCACTGGATTTGTAACAAGGGACACATTTGTTGATTATTGGGTCACTGGAAACATGTTGACGATGGATATAGCTACTCAGATATATTCCATCTTAAAGCAACCAGATCTCAAATACCTCACTCAGGATGAGTTTAAACCTGTCCTCCGAGAACTTTTGGCAACTCATCCAGGGTTGGAGTTCTTGCAGAGCACGCAAGAGTTTCAGGAAAGATATGCTGAAACTGTAATATACAGAATATTTTACTACATAAATAGATCTGGGAACGGTCATCTCACACTCAGGGAGTTGAAGCGTGGAAACTTGATTGATGCAATGCAACATGCAGATGAAGAAGAGGACATCAACAAGGTTTTGAGGTACTTCTCGTATGAGCACTTTTATGTGATATACTGCAAGTTTTGGGAGCTGGATACAGATCATGATTTCCTGATCGACAAAGAGAACCTGATCAGATATGGTAACCATGCACTTACCTACAGGATTGTTGACAGAATATTTTCTCAGGTCGCAAGAAAGTTTACGAGCCAGGTTGAAGGAAAGATGGGGTATCAAGATTTTGTCTACTTCATTCTCTCAGAGGAGGATAAGACTTCTGAGCCTAGCCTTGAGTTCTGGTTCAAGTGCATAGATTTGGATGGAAATGGAGTTTTGACACGGAATGAACTGAATTTCTTTTATGAGGAGCAATTGCATAGAATGGAGTGCATGGCCCAAGAACCTGTTCTCTTTGAGGATATTTTGTGCCAGATAATTGACATGATTGGACCAGAGAATGAGAGCTATATCACACTCCATGATCTGAAAGGCTGTAAGCTTTCAGGAAGTGTTTTCAATATCCTTTTCAACCTTAACAAGTTCATGGCCTTTGAAACTCGTGATCCATTTCTCATTCGTCAGGAACGTGAGAATCCAACCTTGACAGAGTGGGATCGATTTGCACATAGAGAATACATCAGACTTTCAATGGAAGAAGATGTTGAAGATGCCTCAAATGGAAGTGCCGAAGTTTGGGACGAATCACTAGAGGCTCCCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03015 ko04071 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04151 map04152 map04261 map04728 map05165

Pfam Domains

Protein Families

EF-hand_5 EF-hand_7

Protein Analysis

436

Amino Acids

51.26

Weight (kDa)

4.95

Isoelectric Point (pI)

35.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_13	PF17958	112 - 201	3.1e-32	EF-hand domain
EF-hand_7	PF13499	217 - 315	3.6e-10	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54450 AT5G28850 AT5G28850 AT5G28900
fragaria_vesca	FvH4_6g01930
malus_domestica	MD04G1230600.v1.1 MD12G1249000.v1.1
prunus_persica	Prupe.6G350400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0449671 RchiOBHm_Chr3g0449721
rosa_laevigata	RLG00000025807 RLG00000025809
rosa_multiflora	Rmu_sc0009629.1_g000003 Rmu_sc0009629.1_g000010 Rmu_sc0010999.1_g000003
rosa_roxburghii	Rroxscaffold_6G00427570 Rroxscaffold_6G00427590
rosa_rugosa	Rorug02G0622700
rosa_samantha	Rh3AG020800 Rh3AG021100 Rh3BG021500 Rh3BG021800 Rh3CG020000 Rh3DG021300 Rh3DG021600
rosa_wichuraiana	Rw3G001720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	831
AclWI	GGATC	2 cut(s)	1142, 1206
AcsI	RAATTY	2 cut(s)	188, 937
AfaI	GTAC	1 cut(s)	629
AfiI	CCNNNNNNNGG	2 cut(s)	391, 1278
AflIII	ACRYGT	1 cut(s)	321
AgsI	TTSAA	9 cut(s)	250, 559, 803, 889, 1102, 1114, 1139, 1233, 1250
AjnI	CCWGG	2 cut(s)	436, 795
AjuI	GAANNNNNNNTTGG	2 cut(s)	975, 1007
AluBI	AGCT	5 cut(s)	220, 341, 676, 1050, 1084
AluI	AGCT	5 cut(s)	220, 341, 676, 1050, 1084
Alw21I	GWGCWC	2 cut(s)	461, 645
AlwI	GGATC	2 cut(s)	1142, 1206
AoxI	GGCC	2 cut(s)	978, 1131
ApeKI	GCWGC	1 cut(s)	220
ApoI	RAATTY	2 cut(s)	188, 937
Asp700I	GAANNNNTTC	2 cut(s)	766, 990
AspS9I	GGNCC	3 cut(s)	17, 979, 1034
AvaII	GGWCC	2 cut(s)	17, 1034
BaeI	ACNNNNGTAYC	2 cut(s)	65, 98
BauI	CACGAG	1 cut(s)	1143
Bbv12I	GWGCWC	2 cut(s)	461, 645
BbvI	GCAGC	1 cut(s)	207
BccI	CCATC	5 cut(s)	164, 325, 365, 805, 899
BciT130I	CCWGG	2 cut(s)	438, 797
BciVI	GTATCC	1 cut(s)	673
BclI	TGATCA	1 cut(s)	717
BfaI	CTAG	2 cut(s)	870, 1292
BfmI	CTRYAG	2 cut(s)	176, 745
BfuI	GTATCC	1 cut(s)	673
BglII	AGATCT	2 cut(s)	373, 524
BisI	GCNGC	1 cut(s)	221
BlsI	GCNGC	1 cut(s)	222
Bme1390I	CCNGG	2 cut(s)	438, 797
Bme18I	GGWCC	2 cut(s)	17, 1034
BmgT120I	GGNCC	3 cut(s)	17, 979, 1034
BmiI	GGNNCC	1 cut(s)	1299
BmrFI	CCNGG	2 cut(s)	438, 797
BmsI	GCATC	4 cut(s)	171, 250, 568, 1243
Bpu10I	CCTNAGC	1 cut(s)	204
BpuEI	CTTGAG	1 cut(s)	896
Bsa29I	ATCGAT	1 cut(s)	1201
BsaBI	GATNNNNATC	1 cut(s)	684
BsaJI	CCNNGG	1 cut(s)	437
BsaXI	ACNNNNNCTCC	2 cut(s)	396, 426
Bsc4I	CCNNNNNNNGG	2 cut(s)	391, 1278
Bse118I	RCCGGY	1 cut(s)	58
Bse1I	ACTGG	2 cut(s)	277, 319
Bse3DI	GCAATG	1 cut(s)	588
Bse8I	GATNNNNATC	1 cut(s)	684
BseBI	CCWGG	2 cut(s)	438, 797
BseCI	ATCGAT	1 cut(s)	1201
BseDI	CCNNGG	1 cut(s)	437
BseGI	GGATG	3 cut(s)	400, 433, 910
BseJI	GATNNNNATC	1 cut(s)	684
BseLI	CCNNNNNNNGG	2 cut(s)	391, 1278
BseMI	GCAATG	1 cut(s)	588
BseMII	CTCAG	8 cut(s)	92, 180, 359, 404, 562, 785, 855, 858
BseNI	ACTGG	2 cut(s)	277, 319
BseRI	GAGGAG	2 cut(s)	863, 965
BseXI	GCAGC	1 cut(s)	207
BshFI	GGCC	2 cut(s)	980, 1133
BshVI	ATCGAT	1 cut(s)	1201
BsiHKAI	GWGCWC	2 cut(s)	461, 645
BsiSI	CCGG	1 cut(s)	59
BslFI	GGGAC	2 cut(s)	302, 1295
BslI	CCNNNNNNNGG	2 cut(s)	391, 1278
BsmFI	GGGAC	2 cut(s)	302, 1295
BsnI	GGCC	2 cut(s)	980, 1133
Bsp1286I	GDGCHC	2 cut(s)	461, 645
Bsp143I	GATC	9 cut(s)	124, 373, 524, 685, 699, 717, 1066, 1147, 1198
BspANI	GGCC	2 cut(s)	980, 1133
BspCNI	CTCAG	8 cut(s)	91, 179, 358, 403, 561, 784, 856, 857
BspDI	ATCGAT	1 cut(s)	1201
BspHI	TCATGA	1 cut(s)	688
BspLI	GGNNCC	1 cut(s)	1299
BspMAI	CTGCAG	1 cut(s)	180
BspPI	GGATC	2 cut(s)	1142, 1206
BsrDI	GCAATG	1 cut(s)	588
BsrFI	RCCGGY	1 cut(s)	58
BsrI	ACTGG	2 cut(s)	277, 319
BssAI	RCCGGY	1 cut(s)	58
BssECI	CCNNGG	1 cut(s)	437
BssMI	GATC	9 cut(s)	124, 373, 524, 685, 699, 717, 1066, 1147, 1198
BssSI	CACGAG	1 cut(s)	1143
Bst2BI	CACGAG	1 cut(s)	1143
Bst2UI	CCWGG	2 cut(s)	438, 797
Bst4CI	ACNGT	2 cut(s)	493, 536
Bst6I	CTCTTC	2 cut(s)	251, 597
BstC8I	GCNNGC	3 cut(s)	56, 218, 461
BstDEI	CTNAG	9 cut(s)	78, 166, 204, 345, 390, 548, 771, 844, 864
BstEII	GGTNACC	1 cut(s)	728
BstENI	CCTNNNNNAGG	1 cut(s)	389
BstF5I	GGATG	3 cut(s)	400, 433, 910
BstKTI	GATC	9 cut(s)	127, 376, 527, 688, 702, 720, 1069, 1150, 1201
BstMBI	GATC	9 cut(s)	124, 373, 524, 685, 699, 717, 1066, 1147, 1198
BstMWI	GCNNNNNNNGC	1 cut(s)	460
BstNI	CCWGG	2 cut(s)	438, 797
BstNSI	RCATGY	2 cut(s)	325, 593
BstPI	GGTNACC	1 cut(s)	728
BstSCI	CCNGG	2 cut(s)	436, 795
BstSFI	CTRYAG	2 cut(s)	176, 745
BstV1I	GCAGC	1 cut(s)	207
BstX2I	RGATCY	2 cut(s)	373, 524
BstYI	RGATCY	2 cut(s)	373, 524
Bsu15I	ATCGAT	1 cut(s)	1201
BsuI	GTATCC	1 cut(s)	673
BsuRI	GGCC	2 cut(s)	980, 1133
BsuTUI	ATCGAT	1 cut(s)	1201
BtsCI	GGATG	3 cut(s)	400, 433, 910
BtsIMutI	CAGTG	2 cut(s)	270, 312
Cac8I	GCNNGC	3 cut(s)	56, 218, 461
CciI	TCATGA	1 cut(s)	688
Cfr10I	RCCGGY	1 cut(s)	58
Cfr13I	GGNCC	3 cut(s)	17, 979, 1034
ClaI	ATCGAT	1 cut(s)	1201
Csp6I	GTAC	1 cut(s)	628
CviQI	GTAC	1 cut(s)	628
DdeI	CTNAG	9 cut(s)	78, 166, 204, 345, 390, 548, 771, 844, 864
DpnI	GATC	9 cut(s)	126, 375, 526, 687, 701, 719, 1068, 1149, 1200
DpnII	GATC	9 cut(s)	124, 373, 524, 685, 699, 717, 1066, 1147, 1198
DraI	TTTAAA	2 cut(s)	193, 403
Eam1104I	CTCTTC	2 cut(s)	251, 597
EarI	CTCTTC	2 cut(s)	251, 597
Eco32I	GATATC	1 cut(s)	211
Eco47I	GGWCC	2 cut(s)	17, 1034
Eco91I	GGTNACC	1 cut(s)	728
EcoNI	CCTNNNNNAGG	1 cut(s)	389
EcoO65I	GGTNACC	1 cut(s)	728
EcoRII	CCWGG	2 cut(s)	436, 795
EcoRV	GATATC	1 cut(s)	211
EcoT22I	ATGCAT	1 cut(s)	186
FaqI	GGGAC	2 cut(s)	302, 1295
FbaI	TGATCA	1 cut(s)	717
FblI	GTMKAC	1 cut(s)	831
Fnu4HI	GCNGC	1 cut(s)	221
FokI	GGATG	3 cut(s)	407, 420, 917
Fsp4HI	GCNGC	1 cut(s)	221
FspBI	CTAG	2 cut(s)	870, 1292
GluI	GCNGC	1 cut(s)	221
HaeIII	GGCC	2 cut(s)	980, 1133
HapII	CCGG	1 cut(s)	59
HincII	GTYRAC	2 cut(s)	327, 757
HindII	GTYRAC	2 cut(s)	327, 757
HindIII	AAGCTT	1 cut(s)	1082
HinfI	GANTC	3 cut(s)	141, 1177, 1286
HpaII	CCGG	1 cut(s)	59
Hpy166II	GTNNAC	4 cut(s)	327, 757, 789, 832
Hpy188I	TCNGA	8 cut(s)	169, 348, 416, 722, 847, 865, 1071, 1226
Hpy188III	TCNNGA	8 cut(s)	392, 473, 689, 697, 821, 1089, 1145, 1166
Hpy8I	GTNNAC	4 cut(s)	327, 757, 789, 832
HpyAV	CCTTC	3 cut(s)	30, 234, 797
HpyCH4III	ACNGT	2 cut(s)	493, 536
HpyCH4IV	ACGT	1 cut(s)	1171
HpyF10VI	GCNNNNNNNGC	1 cut(s)	460
HpyF3I	CTNAG	9 cut(s)	78, 166, 204, 345, 390, 548, 771, 844, 864
HpySE526I	ACGT	1 cut(s)	1171
Ksp22I	TGATCA	1 cut(s)	717
Kzo9I	GATC	9 cut(s)	124, 373, 524, 685, 699, 717, 1066, 1147, 1198
LmnI	GCTCC	3 cut(s)	673, 952, 1303
Lsp1109I	GCAGC	1 cut(s)	207
LweI	GCATC	4 cut(s)	171, 250, 568, 1243
MaeI	CTAG	2 cut(s)	870, 1292
MaeII	ACGT	1 cut(s)	1171
MaeIII	GTNAC	3 cut(s)	280, 310, 728
MalI	GATC	9 cut(s)	126, 375, 526, 687, 701, 719, 1068, 1149, 1200
MboI	GATC	9 cut(s)	124, 373, 524, 685, 699, 717, 1066, 1147, 1198
MboII	GAAGA	7 cut(s)	223, 238, 611, 614, 1250, 1253, 1262
MfeI	CAATTG	1 cut(s)	956
MflI	RGATCY	2 cut(s)	373, 524
MhlI	GDGCHC	2 cut(s)	461, 645
MluCI	AATT	4 cut(s)	188, 937, 956, 1020
MmeI	TCCRAC	2 cut(s)	423, 1205
Mph1103I	ATGCAT	1 cut(s)	186
MroXI	GAANNNNTTC	2 cut(s)	766, 990
MseI	TTAA	5 cut(s)	63, 192, 362, 402, 1119
MslI	CAYNNNNRTG	2 cut(s)	594, 648
MspI	CCGG	1 cut(s)	59
MspR9I	CCNGG	2 cut(s)	438, 797
MssI	GTTTAAAC	1 cut(s)	403
MunI	CAATTG	1 cut(s)	956
MvaI	CCWGG	2 cut(s)	438, 797
MwoI	GCNNNNNNNGC	1 cut(s)	460
NdeII	GATC	9 cut(s)	124, 373, 524, 685, 699, 717, 1066, 1147, 1198
NlaIV	GGNNCC	1 cut(s)	1299
NmuCI	GTSAC	1 cut(s)	310
NsiI	ATGCAT	1 cut(s)	186
NspI	RCATGY	2 cut(s)	325, 593
PagI	TCATGA	1 cut(s)	688
PciI	ACATGT	1 cut(s)	321
PcsI	WCGNNNNNNNCGW	1 cut(s)	1168
PdmI	GAANNNNTTC	2 cut(s)	766, 990
PfeI	GAWTC	3 cut(s)	141, 1177, 1286
PkrI	GCNGC	1 cut(s)	222
PmeI	GTTTAAAC	1 cut(s)	403
PscI	ACATGT	1 cut(s)	321
Psp6I	CCWGG	2 cut(s)	436, 795
PspEI	GGTNACC	1 cut(s)	728
PspGI	CCWGG	2 cut(s)	436, 795
PspN4I	GGNNCC	1 cut(s)	1299
PspPI	GGNCC	3 cut(s)	17, 979, 1034
PstI	CTGCAG	1 cut(s)	180
PsuI	RGATCY	2 cut(s)	373, 524
RsaI	GTAC	1 cut(s)	629
RsaNI	GTAC	1 cut(s)	628
RseI	CAYNNNNRTG	2 cut(s)	594, 648
SaqAI	TTAA	5 cut(s)	63, 192, 362, 402, 1119
SatI	GCNGC	1 cut(s)	221
Sau3AI	GATC	9 cut(s)	124, 373, 524, 685, 699, 717, 1066, 1147, 1198
Sau96I	GGNCC	3 cut(s)	17, 979, 1034
ScrFI	CCNGG	2 cut(s)	438, 797
SduI	GDGCHC	2 cut(s)	461, 645
SfaNI	GCATC	4 cut(s)	171, 250, 568, 1243
SfcI	CTRYAG	2 cut(s)	176, 745
SinI	GGWCC	2 cut(s)	17, 1034
SmiI	ATTTAAAT	1 cut(s)	193
SmiMI	CAYNNNNRTG	2 cut(s)	594, 648
SmlI	CTYRAG	1 cut(s)	875
SmoI	CTYRAG	1 cut(s)	875
Sse9I	AATT	4 cut(s)	188, 937, 956, 1020
SspI	AATATT	2 cut(s)	507, 765
SspMI	CTAG	2 cut(s)	870, 1292
StyD4I	CCNGG	2 cut(s)	436, 795
SwaI	ATTTAAAT	1 cut(s)	193
TaaI	ACNGT	2 cut(s)	493, 536
TaiI	ACGT	1 cut(s)	1174
TaqI	TCGA	2 cut(s)	702, 1201
TasI	AATT	4 cut(s)	188, 937, 956, 1020
TfiI	GAWTC	3 cut(s)	141, 1177, 1286
Tru1I	TTAA	5 cut(s)	63, 192, 362, 402, 1119
Tru9I	TTAA	5 cut(s)	63, 192, 362, 402, 1119
TscAI	CASTG	2 cut(s)	277, 319
TseFI	GTSAC	1 cut(s)	310
TseI	GCWGC	1 cut(s)	220
Tsp45I	GTSAC	1 cut(s)	310
TspDTI	ATGAA	8 cut(s)	17, 129, 201, 216, 612, 826, 945, 1117
TspGWI	ACGGA	1 cut(s)	940
TspRI	CASTG	2 cut(s)	277, 319
VpaK11BI	GGWCC	2 cut(s)	17, 1034
XagI	CCTNNNNNAGG	1 cut(s)	389
XapI	RAATTY	2 cut(s)	188, 937
XceI	RCATGY	2 cut(s)	325, 593
XmiI	GTMKAC	1 cut(s)	831
XmnI	GAANNNNTTC	2 cut(s)	766, 990
XspI	CTAG	2 cut(s)	870, 1292
Zsp2I	ATGCAT	1 cut(s)	186

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.