Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006488

Physical Location & Seq

Genomic Coordinates Forward (+)

31857463 .. 31863509

Gene Structure 6047 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1249000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1620 bp

ATGACTATGGAAATCGTGGTGGATGCGGCGTCGTTTGACGCCGAGCTGTTGCAGCTCAATGAGGTGTCCCCTTTGGCCCTCAAATCGAATCCGAGCTACGTCGAGTCGCTATTCGAGCAGTGGCTTTCGCTTCCCGATACTAATCGCCTGGTGACACAACTGCTAACTGAAGCAAAGGCAGGTAGGCCCCTGAATGTTCAGGGAAATTCTTCTAGTCCACATGCCACTGCAGGCAGTACTTTGCCTTCTATGTTTCCAGCAGGCAGTGCACCACCACTGTCACCAAGAAGTTCATCTGGATCCCCTCGCGTTATGAAACAGAGGATTGGTCCTTCTCATTTAGGTTCTCCCCTGAAATTAGTTAGCGAACCAGTTAAAGAAGTGATACCTCAGTTTTACTTTCTAAATGGTCGCCCGCCTCCAAATGAACTAAAAGATCAATGCTTATTTAGAATCAATCAGTTTTTCTCTGGCAACTCAGACGGACTGCAGATTCATGAATTTAAATCAGTTGCTAAGGATATCTGCAAGCTGCCCTCATTCTTCTCCACATCTCTTTTCAGAAAGATTGATGCTAACAGCACTGGATTTGTAACAAGGGAAACATTTATTGATTATTGGGTTGGTGGAAACATGTTGACGATGGATGTAGCTACTCAGATCTATTCGATCTTAAAGCAACCAGATCTCAAATACCTCACTCAGGATGACTTTAAACCTGTCCTTCGAGAACTTTTGGCAACTCATCCAGGGTTGGAGTTCTTACAGAGCACGCATGAGTTTCAGGAAAGATATGCTGAAACTGTAATATACAGAATATTTTACTACATAAATAGATCTGGGAACGGTCATCTCACACTCAGGGAGTTAAAGCGTGGAAACTTGATCGATGCAATGCAACATGCAGATGAAGAAGAGGACATCAACAAGGTTTTGAGGTACTTCTCTTATGAGCACTTTTATGTGATATACTGCAAGTTTTGGGAGCTGGATACAGATCATGATTTTCTGATCGATAAAGAGAACCTGATCAGATATGGTAACCATGCACTTACCTACCGGATTGTTGACAGAATATTTTCCCAGGTCGCAAGAAAGTTTACTAGCAAGGTTGAAGGAAAGATGGGATATCAAGATTTTGTCAACTTCATTCTCTCAGAGGAGGATAAATCTTCTCCGCCTAGCCTTGAGTTCTGGTTCAAGTGCATAGATTTGGATGGAAATGGAATTTTGACGCGGAATGAACTGCAGTTCTTTTATGAGGAGCAATTGCATAGAATGGAATGCATGGCCCAAGAACCTGTTCTGTTTGAGGATATTTTGTGCCAGATAATTGACATGATTGGACCAGAGAATGATAGCTACATCACACTCCATGATCTGAAAGGCTGTAAGCTTTCAGGAAGTGTTTTCAATATCCTTTTCAACCTTAACAAGTTCATGGCCTTCGAAACTCGTGATCCATTTCTCATTCGTCAGGAACGTGAGAATCCAACCTTGACAGAGTGGGATCGATTTGCACATAGAGAATACATCAGACTTTCAATGGAAGAAGATGTTGAAGATGCCTCCAATGGAAGTGCCGAAGTTTGGGACGAATCACTAGAGGCTCCCTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko03015 ko04071 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04151 map04152 map04261 map04728 map05165

Pfam Domains

Protein Families

EF-hand_5 EF-hand_7

Protein Analysis

540

Amino Acids

62.09

Weight (kDa)

5.0

Isoelectric Point (pI)

40.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_13	PF17958	216 - 305	9.2e-33	EF-hand domain
EF-hand_7	PF13499	321 - 420	1.5e-09	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54450 AT5G28850 AT5G28850 AT5G28900
fragaria_vesca	FvH4_6g01930
malus_domestica	MD04G1230600.v1.1 MD12G1249000.v1.1
prunus_persica	Prupe.6G350400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0449671 RchiOBHm_Chr3g0449721
rosa_laevigata	RLG00000025807 RLG00000025809
rosa_multiflora	Rmu_sc0009629.1_g000003 Rmu_sc0009629.1_g000010 Rmu_sc0010999.1_g000003
rosa_roxburghii	Rroxscaffold_6G00427570 Rroxscaffold_6G00427590
rosa_rugosa	Rorug02G0622700
rosa_samantha	Rh3AG020800 Rh3AG021100 Rh3BG021500 Rh3BG021800 Rh3CG020000 Rh3DG021300 Rh3DG021600
rosa_wichuraiana	Rw3G001720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	170
AccII	CGCG	2 cut(s)	309, 1237
AciI	CCGC	4 cut(s)	26, 416, 1178, 1237
AclWI	GGATC	4 cut(s)	294, 307, 1454, 1518
AcsI	RAATTY	3 cut(s)	205, 500, 1227
AcuI	CTGAAG	1 cut(s)	189
AcyI	GRCGYC	2 cut(s)	29, 39
AfaI	GTAC	2 cut(s)	238, 941
AfiI	CCNNNNNNNGG	2 cut(s)	703, 1590
AflIII	ACRYGT	1 cut(s)	633
AgsI	TTSAA	6 cut(s)	1115, 1201, 1414, 1426, 1545, 1562
AjnI	CCWGG	3 cut(s)	147, 748, 1083
AjuI	GAANNNNNNNTTGG	2 cut(s)	1287, 1319
AluBI	AGCT	8 cut(s)	46, 55, 96, 532, 653, 988, 1362, 1396
AluI	AGCT	8 cut(s)	46, 55, 96, 532, 653, 988, 1362, 1396
Alw21I	GWGCWC	3 cut(s)	271, 773, 957
Alw44I	GTGCAC	1 cut(s)	267
AlwI	GGATC	4 cut(s)	294, 307, 1454, 1518
AoxI	GGCC	4 cut(s)	75, 185, 1290, 1443
ApaLI	GTGCAC	1 cut(s)	267
ApeKI	GCWGC	2 cut(s)	52, 532
ApoI	RAATTY	3 cut(s)	205, 500, 1227
Asp700I	GAANNNNTTC	3 cut(s)	208, 1078, 1302
AspS9I	GGNCC	5 cut(s)	76, 186, 329, 1291, 1346
AsuHPI	GGTGA	2 cut(s)	163, 273
AsuII	TTCGAA	1 cut(s)	1449
AvaII	GGWCC	2 cut(s)	329, 1346
BaeGI	GKGCMC	1 cut(s)	271
BaeI	ACNNNNGTAYC	2 cut(s)	377, 410
BamHI	GGATCC	1 cut(s)	299
BarI	GAAGNNNNNNTAC	2 cut(s)	229, 261
BauI	CACGAG	1 cut(s)	1455
Bbv12I	GWGCWC	3 cut(s)	271, 773, 957
BbvI	GCAGC	2 cut(s)	64, 519
BccI	CCATC	3 cut(s)	637, 1117, 1211
BciT130I	CCWGG	3 cut(s)	149, 750, 1085
BciVI	GTATCC	1 cut(s)	985
BclI	TGATCA	1 cut(s)	1029
BfaI	CTAG	4 cut(s)	213, 1104, 1182, 1604
BfmI	CTRYAG	3 cut(s)	228, 488, 1247
BfuAI	ACCTGC	1 cut(s)	170
BfuI	GTATCC	1 cut(s)	985
BglII	AGATCT	3 cut(s)	660, 685, 836
BisI	GCNGC	3 cut(s)	27, 53, 533
BlsI	GCNGC	3 cut(s)	28, 54, 534
BmcAI	AGTACT	1 cut(s)	238
Bme1390I	CCNGG	3 cut(s)	149, 750, 1085
Bme18I	GGWCC	2 cut(s)	329, 1346
BmgT120I	GGNCC	5 cut(s)	76, 186, 329, 1291, 1346
BmiI	GGNNCC	3 cut(s)	188, 301, 1611
BmrFI	CCNGG	3 cut(s)	149, 750, 1085
BmsI	GCATC	4 cut(s)	13, 562, 880, 1555
Bpu10I	CCTNAGC	1 cut(s)	516
Bpu14I	TTCGAA	1 cut(s)	1449
BpuEI	CTTGAG	1 cut(s)	1208
Bsa29I	ATCGAT	3 cut(s)	888, 1014, 1513
BsaBI	GATNNNNATC	2 cut(s)	141, 996
BsaHI	GRCGYC	2 cut(s)	29, 39
BsaJI	CCNNGG	2 cut(s)	749, 1083
BsaWI	WCCGGW	1 cut(s)	1059
Bsc4I	CCNNNNNNNGG	2 cut(s)	703, 1590
Bse1I	ACTGG	2 cut(s)	371, 589
Bse3DI	GCAATG	1 cut(s)	900
Bse8I	GATNNNNATC	2 cut(s)	141, 996
BseBI	CCWGG	3 cut(s)	149, 750, 1085
BseCI	ATCGAT	3 cut(s)	888, 1014, 1513
BseDI	CCNNGG	2 cut(s)	749, 1083
BseGI	GGATG	5 cut(s)	28, 652, 712, 745, 1222
BseJI	GATNNNNATC	2 cut(s)	141, 996
BseLI	CCNNNNNNNGG	2 cut(s)	703, 1590
BseMI	GCAATG	1 cut(s)	900
BseMII	CTCAG	6 cut(s)	404, 492, 671, 716, 874, 1170
BseNI	ACTGG	2 cut(s)	371, 589
BseRI	GAGGAG	2 cut(s)	1175, 1277
BseSI	GKGCMC	1 cut(s)	271
BseXI	GCAGC	2 cut(s)	64, 519
Bsh1236I	CGCG	2 cut(s)	309, 1237
BshFI	GGCC	4 cut(s)	77, 187, 1292, 1445
BshVI	ATCGAT	3 cut(s)	888, 1014, 1513
BsiHKAI	GWGCWC	3 cut(s)	271, 773, 957
BsiSI	CCGG	1 cut(s)	1060
BslFI	GGGAC	2 cut(s)	52, 1607
BslI	CCNNNNNNNGG	2 cut(s)	703, 1590
BsmFI	GGGAC	2 cut(s)	52, 1607
BsmI	GAATGC	1 cut(s)	1289
BsnI	GGCC	4 cut(s)	77, 187, 1292, 1445
Bsp119I	TTCGAA	1 cut(s)	1449
Bsp1286I	GDGCHC	3 cut(s)	271, 773, 957
BspACI	CCGC	4 cut(s)	26, 416, 1178, 1237
BspANI	GGCC	4 cut(s)	77, 187, 1292, 1445
BspCNI	CTCAG	6 cut(s)	403, 491, 670, 715, 873, 1169
BspDI	ATCGAT	3 cut(s)	888, 1014, 1513
BspFNI	CGCG	2 cut(s)	309, 1237
BspHI	TCATGA	2 cut(s)	496, 1000
BspLI	GGNNCC	3 cut(s)	188, 301, 1611
BspMAI	CTGCAG	3 cut(s)	232, 492, 1251
BspMI	ACCTGC	1 cut(s)	170
BspPI	GGATC	4 cut(s)	294, 307, 1454, 1518
BspT104I	TTCGAA	1 cut(s)	1449
BsrDI	GCAATG	1 cut(s)	900
BsrI	ACTGG	2 cut(s)	371, 589
BssECI	CCNNGG	2 cut(s)	749, 1083
BssNI	GRCGYC	2 cut(s)	29, 39
BssSI	CACGAG	1 cut(s)	1455
Bst2BI	CACGAG	1 cut(s)	1455
Bst2UI	CCWGG	3 cut(s)	149, 750, 1085
Bst4CI	ACNGT	3 cut(s)	279, 805, 848
Bst6I	CTCTTC	1 cut(s)	909
BstACI	GRCGYC	2 cut(s)	29, 39
BstAPI	GCANNNNNTGC	1 cut(s)	266
BstBI	TTCGAA	1 cut(s)	1449
BstC8I	GCNNGC	5 cut(s)	232, 262, 416, 530, 773
BstDEI	CTNAG	7 cut(s)	390, 478, 516, 657, 702, 860, 1156
BstEII	GGTNACC	1 cut(s)	1040
BstENI	CCTNNNNNAGG	1 cut(s)	701
BstF5I	GGATG	5 cut(s)	28, 652, 712, 745, 1222
BstFNI	CGCG	2 cut(s)	309, 1237
BstMWI	GCNNNNNNNGC	3 cut(s)	52, 115, 266
BstNI	CCWGG	3 cut(s)	149, 750, 1085
BstNSI	RCATGY	3 cut(s)	224, 637, 905
BstPI	GGTNACC	1 cut(s)	1040
BstSCI	CCNGG	3 cut(s)	147, 748, 1083
BstSFI	CTRYAG	3 cut(s)	228, 488, 1247
BstSLI	GKGCMC	1 cut(s)	271
BstUI	CGCG	2 cut(s)	309, 1237
BstV1I	GCAGC	2 cut(s)	64, 519
BstX2I	RGATCY	4 cut(s)	299, 660, 685, 836
BstYI	RGATCY	4 cut(s)	299, 660, 685, 836
Bsu15I	ATCGAT	3 cut(s)	888, 1014, 1513
BsuI	GTATCC	1 cut(s)	985
BsuRI	GGCC	4 cut(s)	77, 187, 1292, 1445
BsuTUI	ATCGAT	3 cut(s)	888, 1014, 1513
BtsCI	GGATG	5 cut(s)	28, 652, 712, 745, 1222
BtsI	GCAGTG	3 cut(s)	125, 225, 271
BtsIMutI	CAGTG	5 cut(s)	125, 225, 271, 275, 582
BveI	ACCTGC	1 cut(s)	170
Cac8I	GCNNGC	5 cut(s)	232, 262, 416, 530, 773
CciI	TCATGA	2 cut(s)	496, 1000
Cfr13I	GGNCC	5 cut(s)	76, 186, 329, 1291, 1346
ClaI	ATCGAT	3 cut(s)	888, 1014, 1513
CseI	GACGC	3 cut(s)	18, 47, 1243
Csp6I	GTAC	2 cut(s)	237, 940
CviQI	GTAC	2 cut(s)	237, 940
DdeI	CTNAG	7 cut(s)	390, 478, 516, 657, 702, 860, 1156
DraI	TTTAAA	2 cut(s)	505, 715
Eam1104I	CTCTTC	1 cut(s)	909
EarI	CTCTTC	1 cut(s)	909
EciI	GGCGGA	1 cut(s)	1167
Eco32I	GATATC	2 cut(s)	523, 1130
Eco47I	GGWCC	2 cut(s)	329, 1346
Eco57I	CTGAAG	1 cut(s)	189
Eco91I	GGTNACC	1 cut(s)	1040
EcoNI	CCTNNNNNAGG	1 cut(s)	701
EcoO109I	RGGNCCY	1 cut(s)	186
EcoO65I	GGTNACC	1 cut(s)	1040
EcoRII	CCWGG	3 cut(s)	147, 748, 1083
EcoRV	GATATC	2 cut(s)	523, 1130
EcoT22I	ATGCAT	1 cut(s)	1289
FaqI	GGGAC	2 cut(s)	52, 1607
FauI	CCCGC	1 cut(s)	423
FbaI	TGATCA	1 cut(s)	1029
Fnu4HI	GCNGC	3 cut(s)	27, 53, 533
FokI	GGATG	5 cut(s)	35, 659, 719, 732, 1229
Fsp4HI	GCNGC	3 cut(s)	27, 53, 533
FspBI	CTAG	4 cut(s)	213, 1104, 1182, 1604
GluI	GCNGC	3 cut(s)	27, 53, 533
HaeIII	GGCC	4 cut(s)	77, 187, 1292, 1445
HapII	CCGG	1 cut(s)	1060
HgaI	GACGC	3 cut(s)	18, 47, 1243
Hin1I	GRCGYC	2 cut(s)	29, 39
HincII	GTYRAC	3 cut(s)	639, 1069, 1144
HindII	GTYRAC	3 cut(s)	639, 1069, 1144
HindIII	AAGCTT	1 cut(s)	1394
HinfI	GANTC	6 cut(s)	88, 104, 453, 493, 1489, 1598
HpaII	CCGG	1 cut(s)	1060
HphI	GGTGA	2 cut(s)	163, 273
Hpy166II	GTNNAC	6 cut(s)	218, 269, 639, 1069, 1101, 1144
Hpy188I	TCNGA	9 cut(s)	93, 481, 563, 660, 1011, 1034, 1159, 1383, 1538
Hpy8I	GTNNAC	6 cut(s)	218, 269, 639, 1069, 1101, 1144
Hpy99I	CGWCG	2 cut(s)	34, 104
HpyAV	CCTTC	5 cut(s)	255, 342, 734, 1109, 1456
HpyCH4III	ACNGT	3 cut(s)	279, 805, 848
HpyCH4IV	ACGT	2 cut(s)	99, 1483
HpyF10VI	GCNNNNNNNGC	3 cut(s)	52, 115, 266
HpyF3I	CTNAG	7 cut(s)	390, 478, 516, 657, 702, 860, 1156
HpySE526I	ACGT	2 cut(s)	99, 1483
Hsp92I	GRCGYC	2 cut(s)	29, 39
Ksp22I	TGATCA	1 cut(s)	1029
LmnI	GCTCC	3 cut(s)	985, 1264, 1615
Lsp1109I	GCAGC	2 cut(s)	64, 519
LweI	GCATC	4 cut(s)	13, 562, 880, 1555
MaeI	CTAG	4 cut(s)	213, 1104, 1182, 1604
MaeII	ACGT	2 cut(s)	99, 1483
MaeIII	GTNAC	4 cut(s)	151, 279, 592, 1040
MboII	GAAGA	8 cut(s)	201, 535, 923, 926, 1164, 1562, 1565, 1574
MfeI	CAATTG	1 cut(s)	1268
MflI	RGATCY	4 cut(s)	299, 660, 685, 836
MhlI	GDGCHC	3 cut(s)	271, 773, 957
MluCI	AATT	6 cut(s)	205, 356, 500, 1227, 1268, 1332
MlyI	GAGTC	1 cut(s)	113
MmeI	TCCRAC	2 cut(s)	735, 1517
Mph1103I	ATGCAT	1 cut(s)	1289
MroXI	GAANNNNTTC	3 cut(s)	208, 1078, 1302
MseI	TTAA	6 cut(s)	375, 504, 674, 714, 869, 1431
MslI	CAYNNNNRTG	2 cut(s)	906, 960
MspI	CCGG	1 cut(s)	1060
MspR9I	CCNGG	3 cut(s)	149, 750, 1085
MunI	CAATTG	1 cut(s)	1268
Mva1269I	GAATGC	1 cut(s)	1289
MvaI	CCWGG	3 cut(s)	149, 750, 1085
MvnI	CGCG	2 cut(s)	309, 1237
MwoI	GCNNNNNNNGC	3 cut(s)	52, 115, 266
NlaIV	GGNNCC	3 cut(s)	188, 301, 1611
NmeAIII	GCCGAG	1 cut(s)	67
NmuCI	GTSAC	2 cut(s)	151, 279
NsiI	ATGCAT	1 cut(s)	1289
NspI	RCATGY	3 cut(s)	224, 637, 905
NspV	TTCGAA	1 cut(s)	1449
PagI	TCATGA	2 cut(s)	496, 1000
PciI	ACATGT	1 cut(s)	633
PcsI	WCGNNNNNNNCGW	1 cut(s)	1480
PctI	GAATGC	1 cut(s)	1289
PdmI	GAANNNNTTC	3 cut(s)	208, 1078, 1302
PfeI	GAWTC	5 cut(s)	88, 453, 493, 1489, 1598
PkrI	GCNGC	3 cut(s)	28, 54, 534
PleI	GAGTC	1 cut(s)	112
PpsI	GAGTC	1 cut(s)	112
PscI	ACATGT	1 cut(s)	633
Psp6I	CCWGG	3 cut(s)	147, 748, 1083
PspEI	GGTNACC	1 cut(s)	1040
PspGI	CCWGG	3 cut(s)	147, 748, 1083
PspN4I	GGNNCC	3 cut(s)	188, 301, 1611
PspPI	GGNCC	5 cut(s)	76, 186, 329, 1291, 1346
PstI	CTGCAG	3 cut(s)	232, 492, 1251
PsuI	RGATCY	4 cut(s)	299, 660, 685, 836
RsaI	GTAC	2 cut(s)	238, 941
RsaNI	GTAC	2 cut(s)	237, 940
RseI	CAYNNNNRTG	2 cut(s)	906, 960
SaqAI	TTAA	6 cut(s)	375, 504, 674, 714, 869, 1431
SatI	GCNGC	3 cut(s)	27, 53, 533
Sau96I	GGNCC	5 cut(s)	76, 186, 329, 1291, 1346
ScaI	AGTACT	1 cut(s)	238
SchI	GAGTC	1 cut(s)	113
ScrFI	CCNGG	3 cut(s)	149, 750, 1085
SduI	GDGCHC	3 cut(s)	271, 773, 957
SfaNI	GCATC	4 cut(s)	13, 562, 880, 1555
SfcI	CTRYAG	3 cut(s)	228, 488, 1247
SfuI	TTCGAA	1 cut(s)	1449
SinI	GGWCC	2 cut(s)	329, 1346
SmiI	ATTTAAAT	1 cut(s)	505
SmiMI	CAYNNNNRTG	2 cut(s)	906, 960
SmlI	CTYRAG	1 cut(s)	1187
SmoI	CTYRAG	1 cut(s)	1187
Sse9I	AATT	6 cut(s)	205, 356, 500, 1227, 1268, 1332
SsiI	CCGC	4 cut(s)	26, 416, 1178, 1237
SspI	AATATT	2 cut(s)	819, 1077
SspMI	CTAG	4 cut(s)	213, 1104, 1182, 1604
StyD4I	CCNGG	3 cut(s)	147, 748, 1083
SwaI	ATTTAAAT	1 cut(s)	505
TaaI	ACNGT	3 cut(s)	279, 805, 848
TaiI	ACGT	2 cut(s)	102, 1486
TaqI	TCGA	9 cut(s)	86, 102, 114, 668, 727, 888, 1014, 1449, 1513
TasI	AATT	6 cut(s)	205, 356, 500, 1227, 1268, 1332
TatI	WGTACW	1 cut(s)	236
TauI	GCSGC	1 cut(s)	29
TfiI	GAWTC	5 cut(s)	88, 453, 493, 1489, 1598
Tru1I	TTAA	6 cut(s)	375, 504, 674, 714, 869, 1431
Tru9I	TTAA	6 cut(s)	375, 504, 674, 714, 869, 1431
TscAI	CASTG	5 cut(s)	125, 232, 271, 282, 589
TseFI	GTSAC	2 cut(s)	151, 279
TseI	GCWGC	2 cut(s)	52, 532
Tsp45I	GTSAC	2 cut(s)	151, 279
TspDTI	ATGAA	9 cut(s)	282, 329, 441, 485, 513, 924, 1138, 1257, 1429
TspGWI	ACGGA	1 cut(s)	498
TspRI	CASTG	5 cut(s)	125, 232, 271, 282, 589
VneI	GTGCAC	1 cut(s)	267
VpaK11BI	GGWCC	2 cut(s)	329, 1346
XagI	CCTNNNNNAGG	1 cut(s)	701
XapI	RAATTY	3 cut(s)	205, 500, 1227
XceI	RCATGY	3 cut(s)	224, 637, 905
XmnI	GAANNNNTTC	3 cut(s)	208, 1078, 1302
XspI	CTAG	4 cut(s)	213, 1104, 1182, 1604
ZrmI	AGTACT	1 cut(s)	238
Zsp2I	ATGCAT	1 cut(s)	1289

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.