AT1G55840

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009845
Physical Location & Seq
Forward (+)
20873425 .. 20876346
2922 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G55840.1

Sequence Viewer

Length: 978 bp
ATGAGCATCACTAATGAAGAAGCAGTGAAGCAATTGCGTGCTTTAATGGAAGATGTTGATGATTCACTCAGAGAGTCGTATCGGAACATTCATCAAGGGTATCCGACAGAGAATTTGTTACGCTTTCTTAAAGCCAGGGATGGGAATGTCCAGAAAGCTCATAAAATGTTGCTCGAGTGTTTGGAATGGAGGACTCAAAACGAGATTGACAAGATACTAACTAAACCCATTGTTCCTGTTGACCTGTACAGAGGAATCAGAGACACACAACTTGTCGGTGTGTCTGGTTACTCTAAAGAGGGTCTCCCTGTCATTGCCATTGGTGTGGGGCTTAGCACATATGATAAAGCCTCCGTTCACTACTATGTACAGTCTCACATTCAAATGAATGAGTACCGGGATCGTGTTGTATTGCCATCTGCTTCAAAGAAACAGGGACGACCAATTTGCACTTGTTTGAAAATTTTGGATATGTCTGGTTTAAAGCTTTCAGCTTTAAGTCAAATTAAGTTAATGACTGCTATAACAACAATAGATGATTTGAACTATCCAGAGAAGACAGAGACATACTATGTTGTCAATGTCCCGTACATATTCTCTGCTTGTTGGAAAACCATAAAGCCTCTGTTGCAAGAGAGAACAAAGAAGAAGATTCAAGTTCTGAAAGGTTGCGGGAAAGATGAGTTGCTAAAGATAATGGACTATGAGTCTCTCCCACATTTCTGTAGAAGAGAAGGGTCTGGATCTGGTAGGCATATCTCAAATGGAACAGTAGACAATTGTTTCTCTCTGGATCACTCTTTCCACCAAGACCTTTATGATTATGTCAAGCAGCAGGCTCTGGTTAAAGGATCAGGTGCACCGATCAGACATGGTTCAGTCCACGTTAAGTTCCCTGAGCCAGACACCGAAGGCAACAAGATCTTCGATACCTTAGAAAATGAGTTCCAGAAGCTTGGAAATGACCAGAAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

325

Amino Acids

37.0

Weight (kDa)

8.32

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 8 - 58 1.7e-10 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 89 - 241 1.3e-19 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 774
AciI CCGC 1 cut(s) 672
AclWI GGATC 4 cut(s) 408, 751, 801, 859
AcsI RAATTY 2 cut(s) 112, 462
AfaI GTAC 4 cut(s) 248, 369, 395, 590
AfiI CCNNNNNNNGG 1 cut(s) 141
AgsI TTSAA 5 cut(s) 383, 426, 460, 544, 656
AhdI GACNNNNNGTC 1 cut(s) 706
AjnI CCWGG 1 cut(s) 134
AluBI AGCT 4 cut(s) 158, 487, 494, 955
AluI AGCT 4 cut(s) 158, 487, 494, 955
Alw21I GWGCWC 1 cut(s) 862
Alw26I GTCTC 5 cut(s) 255, 308, 378, 557, 714
Alw44I GTGCAC 1 cut(s) 858
AlwI GGATC 4 cut(s) 408, 751, 801, 859
AlwNI CAGNNNCTG 1 cut(s) 841
Ama87I CYCGRG 1 cut(s) 173
ApaLI GTGCAC 1 cut(s) 858
ApeKI GCWGC 1 cut(s) 832
ApoI RAATTY 2 cut(s) 112, 462
ArsI GACNNNNNNTTYG 4 cut(s) 97, 129, 429, 461
AsuC2I CCSGG 1 cut(s) 398
AvaI CYCGRG 1 cut(s) 173
BaeGI GKGCMC 1 cut(s) 862
BbsI GAAGAC 1 cut(s) 563
Bbv12I GWGCWC 1 cut(s) 862
BbvI GCAGC 1 cut(s) 844
BccI CCATC 2 cut(s) 134, 424
BcgI CGANNNNNNTGC 2 cut(s) 429, 463
BciT130I CCWGG 1 cut(s) 136
BciVI GTATCC 1 cut(s) 111
BcnI CCSGG 1 cut(s) 398
BcoDI GTCTC 5 cut(s) 255, 308, 378, 557, 714
BfmI CTRYAG 1 cut(s) 724
BfuI GTATCC 1 cut(s) 111
BglII AGATCT 2 cut(s) 921, 972
BisI GCNGC 1 cut(s) 833
BlpI GCTNAGC 1 cut(s) 332
BlsI GCNGC 1 cut(s) 834
Bme1390I CCNGG 2 cut(s) 136, 398
BmeRI GACNNNNNGTC 1 cut(s) 706
BmeT110I CYCGRG 1 cut(s) 173
BmrFI CCNGG 2 cut(s) 136, 398
BmsI GCATC 1 cut(s) 15
BpiI GAAGAC 1 cut(s) 563
Bpu10I CCTNAGC 1 cut(s) 897
Bpu1102I GCTNAGC 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 398
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 1 cut(s) 135
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse3DI GCAATG 1 cut(s) 312
BseBI CCWGG 1 cut(s) 136
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMI GCAATG 1 cut(s) 312
BseMII CTCAG 2 cut(s) 82, 888
BseSI GKGCMC 1 cut(s) 862
BseXI GCAGC 1 cut(s) 844
BsiHKAI GWGCWC 1 cut(s) 862
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 1 cut(s) 397
BslFI GGGAC 2 cut(s) 450, 569
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 5 cut(s) 255, 308, 378, 557, 714
BsmFI GGGAC 2 cut(s) 450, 569
Bso31I GGTCTC 1 cut(s) 308
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 862
Bsp1407I TGTACA 2 cut(s) 246, 367
Bsp143I GATC 7 cut(s) 400, 743, 793, 851, 864, 921, 972
Bsp1720I GCTNAGC 1 cut(s) 332
BspACI CCGC 1 cut(s) 672
BspCNI CTCAG 2 cut(s) 81, 889
BspPI GGATC 4 cut(s) 408, 751, 801, 859
BspTNI GGTCTC 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 312
BsrGI TGTACA 2 cut(s) 246, 367
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 7 cut(s) 400, 743, 793, 851, 864, 921, 972
Bst2UI CCWGG 1 cut(s) 136
Bst4CI ACNGT 2 cut(s) 372, 772
Bst6I CTCTTC 1 cut(s) 724
BstAUI TGTACA 2 cut(s) 246, 367
BstC8I GCNNGC 2 cut(s) 39, 837
BstDEI CTNAG 4 cut(s) 68, 332, 897, 934
BstF5I GGATG 1 cut(s) 145
BstKTI GATC 7 cut(s) 403, 746, 796, 854, 867, 924, 975
BstMAI GTCTC 5 cut(s) 255, 308, 378, 557, 714
BstMBI GATC 7 cut(s) 400, 743, 793, 851, 864, 921, 972
BstMWI GCNNNNNNNGC 1 cut(s) 628
BstNI CCWGG 1 cut(s) 136
BstSCI CCNGG 2 cut(s) 134, 396
BstSFI CTRYAG 1 cut(s) 724
BstSLI GKGCMC 1 cut(s) 862
BstV1I GCAGC 1 cut(s) 844
BstV2I GAAGAC 1 cut(s) 563
BstX2I RGATCY 3 cut(s) 743, 921, 972
BstXI CCANNNNNNTGG 2 cut(s) 325, 956
BstYI RGATCY 3 cut(s) 743, 921, 972
BsuI GTATCC 1 cut(s) 111
BtsCI GGATG 1 cut(s) 145
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 1 cut(s) 30
Cac8I GCNNGC 2 cut(s) 39, 837
CaiI CAGNNNCTG 1 cut(s) 841
Csp6I GTAC 4 cut(s) 247, 368, 394, 589
CviAII CATG 1 cut(s) 872
CviQI GTAC 4 cut(s) 247, 368, 394, 589
DdeI CTNAG 4 cut(s) 68, 332, 897, 934
DpnI GATC 7 cut(s) 402, 745, 795, 853, 866, 923, 974
DpnII GATC 7 cut(s) 400, 743, 793, 851, 864, 921, 972
DraI TTTAAA 1 cut(s) 483
DriI GACNNNNNGTC 1 cut(s) 706
Eam1104I CTCTTC 1 cut(s) 724
Eam1105I GACNNNNNGTC 1 cut(s) 706
EarI CTCTTC 1 cut(s) 724
Eco31I GGTCTC 1 cut(s) 308
Eco88I CYCGRG 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 134
FaeI CATG 1 cut(s) 875
FaqI GGGAC 2 cut(s) 450, 569
FatI CATG 1 cut(s) 871
FauI CCCGC 1 cut(s) 665
FauNDI CATATG 1 cut(s) 340
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 1 cut(s) 833
FokI GGATG 1 cut(s) 152
Fsp4HI GCNGC 1 cut(s) 833
GluI GCNGC 1 cut(s) 833
HapII CCGG 1 cut(s) 397
Hin1II CATG 1 cut(s) 875
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HindIII AAGCTT 2 cut(s) 485, 953
HinfI GANTC 6 cut(s) 62, 74, 193, 255, 652, 707
HpaII CCGG 1 cut(s) 397
Hpy166II GTNNAC 5 cut(s) 241, 358, 775, 860, 883
Hpy188I TCNGA 7 cut(s) 71, 84, 105, 260, 663, 869, 977
Hpy188III TCNNGA 5 cut(s) 151, 551, 741, 791, 949
Hpy8I GTNNAC 5 cut(s) 241, 358, 775, 860, 883
HpyAV CCTTC 2 cut(s) 728, 905
HpyCH4III ACNGT 2 cut(s) 372, 772
HpyCH4IV ACGT 1 cut(s) 885
HpyCH4V TGCA 3 cut(s) 450, 631, 860
HpyF10VI GCNNNNNNNGC 1 cut(s) 628
HpyF3I CTNAG 4 cut(s) 68, 332, 897, 934
HpySE526I ACGT 1 cut(s) 885
Hsp92II CATG 1 cut(s) 875
Kzo9I GATC 7 cut(s) 400, 743, 793, 851, 864, 921, 972
Lsp1109I GCAGC 1 cut(s) 844
LweI GCATC 1 cut(s) 15
MaeII ACGT 1 cut(s) 885
MaeIII GTNAC 2 cut(s) 117, 287
MalI GATC 7 cut(s) 402, 745, 795, 853, 866, 923, 974
MboI GATC 7 cut(s) 400, 743, 793, 851, 864, 921, 972
MboII GAAGA 7 cut(s) 29, 62, 568, 658, 661, 741, 916
MfeI CAATTG 2 cut(s) 32, 778
MflI RGATCY 3 cut(s) 743, 921, 972
MhlI GDGCHC 1 cut(s) 862
MluCI AATT 6 cut(s) 32, 112, 444, 462, 504, 778
MlyI GAGTC 3 cut(s) 83, 187, 716
MmeI TCCRAC 2 cut(s) 128, 587
MnlI CCTC 5 cut(s) 183, 245, 292, 361, 633
MseI TTAA 8 cut(s) 44, 129, 482, 497, 507, 512, 846, 888
MslI CAYNNNNRTG 3 cut(s) 323, 363, 383
MspI CCGG 1 cut(s) 397
MspR9I CCNGG 2 cut(s) 136, 398
MunI CAATTG 2 cut(s) 32, 778
MvaI CCWGG 1 cut(s) 136
MwoI GCNNNNNNNGC 1 cut(s) 628
NciI CCSGG 1 cut(s) 398
NdeI CATATG 1 cut(s) 340
NdeII GATC 7 cut(s) 400, 743, 793, 851, 864, 921, 972
NlaIII CATG 1 cut(s) 875
PaeR7I CTCGAG 1 cut(s) 173
PfeI GAWTC 3 cut(s) 62, 255, 652
PkrI GCNGC 1 cut(s) 834
PleI GAGTC 3 cut(s) 82, 187, 715
PpsI GAGTC 3 cut(s) 82, 187, 715
Psp6I CCWGG 1 cut(s) 134
PspGI CCWGG 1 cut(s) 134
PspXI VCTCGAGB 1 cut(s) 173
PstNI CAGNNNCTG 1 cut(s) 841
PsuI RGATCY 3 cut(s) 743, 921, 972
RsaI GTAC 4 cut(s) 248, 369, 395, 590
RsaNI GTAC 4 cut(s) 247, 368, 394, 589
RseI CAYNNNNRTG 3 cut(s) 323, 363, 383
SaqAI TTAA 8 cut(s) 44, 129, 482, 497, 507, 512, 846, 888
SatI GCNGC 1 cut(s) 833
Sau3AI GATC 7 cut(s) 400, 743, 793, 851, 864, 921, 972
SchI GAGTC 3 cut(s) 83, 187, 716
ScrFI CCNGG 2 cut(s) 136, 398
SduI GDGCHC 1 cut(s) 862
SfaNI GCATC 1 cut(s) 15
SfcI CTRYAG 1 cut(s) 724
Sfr274I CTCGAG 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 173
SmiMI CAYNNNNRTG 3 cut(s) 323, 363, 383
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 6 cut(s) 32, 112, 444, 462, 504, 778
SsiI CCGC 1 cut(s) 672
StyD4I CCNGG 2 cut(s) 134, 396
TaaI ACNGT 2 cut(s) 372, 772
TaiI ACGT 1 cut(s) 888
TaqI TCGA 2 cut(s) 174, 927
TasI AATT 6 cut(s) 32, 112, 444, 462, 504, 778
TatI WGTACW 2 cut(s) 246, 367
TfiI GAWTC 3 cut(s) 62, 255, 652
Tru1I TTAA 8 cut(s) 44, 129, 482, 497, 507, 512, 846, 888
Tru9I TTAA 8 cut(s) 44, 129, 482, 497, 507, 512, 846, 888
TscAI CASTG 1 cut(s) 30
TseI GCWGC 1 cut(s) 832
TspDTI ATGAA 3 cut(s) 30, 80, 401
TspGWI ACGGA 1 cut(s) 343
TspRI CASTG 1 cut(s) 30
VneI GTGCAC 1 cut(s) 858
XapI RAATTY 2 cut(s) 112, 462
XhoI CTCGAG 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.