AT1G64080

membrane-associated kinase regulator 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015380
Physical Location & Seq
Reverse (-)
23784977 .. 23786800
1824 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G64080.1

Sequence Viewer

Length: 1236 bp
ATGGAAGCTTTCAGTCTCCTTAACTACTGGAAAAACAGTGGTGGTGCTGTTACTTTCCTTCCTCACTCCTCCGAATCTTCTTCTTCTTCTCTTTGCCGTTACTCCGCCGGTGAATCCATCGGAACCACCACCATTGTCACCTCTGTTACCGAAACTGAGGACGAAGAAGCTGATGAAAACGACGACGAAGGACCGTTCTTCGACCTTGAGTTCGCTCTTCCAGCCGAAGAAGAAGACACAGACGGAGGAGTCGGAAGAGAGGGAGACTCCGATTGTACGGACGGTGGTTGTGAGTTTAAATTCACTCTCTCGTCTTGTTCCGGAGGAGAAGATCGAGTAGATCCAGATTCACTCGATGATGTTTTCTTCAAGGAAACGATTGTTGAAGAGGTTGAACCACCGACTTCTTCAGAGCAGCAGACATGTTCTGTTAAAGCTCCCGCGGCGCAGTTATCGGCGTCAATTTTGAAAACCGCCACCAAGCTTCGTGTGTTCATGCTCGGAATGAAGAAATCAAAGCTAATCCAAGCGAAATCAGAAGCTAATTTCGGATCTGAAGACCTCGATAAGCAAACTCATCCTCCTCCGCCGTTATCACTTTCGCCGGAATCGCAACAGAAAAGTACGGTGACAGTGAATTTAAAAGTTGAAGAAGTTCCGATAATTTCTCTATTCTCAAGAGAAAACAGCTCGAAGAACTCATCTTCGTCTTCTTCTTCATCTTCTTCTTCGTCTTCCTCATTAAAGAAGCAAAACGGCAACGAATCTGTCGTTTCAGATGAGAAACGATTCGTGATGATGCAAAAGTACTTAAAGAAGGTAAAACCACTTTACATCAGAGTTTCACGTCGTTACGGCGAGAAATTACGACACTCCGGTCAACTAACAGCTCCATCGTCTGCGAGATCCACGGCGGAGAAAGCAGATTCGCCGGTGGCTACGAAGACTAATAATAAACCGGGGAACAACATCAACATTCCAGCTGGTCTTAAAGTAGTGAGGAAACATCTCGGGAAAAGCAGATCGTCATCTTCAACAGCGACTACACCGTCGTCTACTACGGCGACTGTAACGACTCAGTCTGAGTCAAGAAGACGCGACGACTCGCTTCTTCAACAACAAGATAGTATTCAAAGTGCCATTTTACACTGCAAAAGATCTTTCAATTCCTCTAGAGATAAAGATCCGTCGGTGTTACCAAGATCGGTGAGTGAACCTTCCTCTTACGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

411

Amino Acids

44.51

Weight (kDa)

5.44

Isoelectric Point (pI)

66.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64080
fragaria_vesca FvH4_5g03470
malus_domestica MD06G1094800.v1.1 MD14G1115100.v1.1
prunus_persica Prupe.5G110000_v2.0.a1
pyrus_communis pycom06g09150
rosa_chinensis RchiOBHm_Chr7g0196191
rosa_laevigata RLG00000004053
rosa_multiflora Rmu_sc0007292.1_g000008
rosa_roxburghii Rroxscaffold_3G00259680
rosa_rugosa Rorug07G0032000
rosa_samantha Rh7AG157400 Rh7BG159100 Rh7CG163100 Rh7DG158400
rosa_wichuraiana Rw7G013460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 248, 876, 1048
AccI GTMKAC 1 cut(s) 1055
AccII CGCG 2 cut(s) 443, 1098
AccIII TCCGGA 1 cut(s) 320
AciI CCGC 6 cut(s) 105, 441, 443, 474, 587, 914
AclWI GGATC 4 cut(s) 335, 559, 900, 1178
AcsI RAATTY 2 cut(s) 299, 637
AcuI CTGAAG 2 cut(s) 393, 576
AcyI GRCGYC 1 cut(s) 458
AfaI GTAC 3 cut(s) 277, 625, 809
AflIII ACRYGT 1 cut(s) 422
AgsI TTSAA 9 cut(s) 370, 386, 395, 469, 650, 1035, 1115, 1133, 1165
AjiI CACGTC 1 cut(s) 848
AluBI AGCT 9 cut(s) 8, 170, 437, 484, 520, 542, 690, 890, 983
AluI AGCT 9 cut(s) 8, 170, 437, 484, 520, 542, 690, 890, 983
Alw26I GTCTC 2 cut(s) 20, 258
AlwI GGATC 4 cut(s) 335, 559, 900, 1178
Ama87I CYCGRG 1 cut(s) 1010
Aor13HI TCCGGA 1 cut(s) 320
ApeKI GCWGC 1 cut(s) 415
ApoI RAATTY 2 cut(s) 299, 637
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
Asp700I GAANNNNTTC 2 cut(s) 654, 788
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 1 cut(s) 191
AsuC2I CCSGG 1 cut(s) 960
AsuHPI GGTGA 4 cut(s) 122, 130, 640, 1219
AvaI CYCGRG 1 cut(s) 1010
AvaII GGWCC 1 cut(s) 191
BbsI GAAGAC 6 cut(s) 240, 564, 702, 726, 950, 1099
BbvI GCAGC 1 cut(s) 427
BccI CCATC 2 cut(s) 125, 901
BceAI ACGGC 6 cut(s) 81, 574, 772, 871, 927, 1077
BcnI CCSGG 1 cut(s) 960
BcoDI GTCTC 2 cut(s) 20, 258
BfaI CTAG 1 cut(s) 1173
BglII AGATCT 1 cut(s) 1157
BisI GCNGC 2 cut(s) 416, 444
BlsI GCNGC 2 cut(s) 417, 445
BmcAI AGTACT 1 cut(s) 809
Bme1390I CCNGG 1 cut(s) 960
Bme18I GGWCC 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 1010
BmgBI CACGTC 1 cut(s) 848
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 1 cut(s) 124
BmrFI CCNGG 1 cut(s) 960
BmsI GCATC 1 cut(s) 789
BpiI GAAGAC 6 cut(s) 240, 564, 702, 726, 950, 1099
BplI GAGNNNNNCTC 2 cut(s) 251, 283
BpuEI CTTGAG 2 cut(s) 227, 661
BpuMI CCSGG 1 cut(s) 960
BsaBI GATNNNNATC 2 cut(s) 1027, 1182
BsaHI GRCGYC 1 cut(s) 458
BsaJI CCNNGG 3 cut(s) 441, 909, 959
BsaWI WCCGGW 2 cut(s) 320, 875
BsaXI ACNNNNNCTCC 2 cut(s) 565, 595
Bse118I RCCGGY 2 cut(s) 107, 931
Bse1I ACTGG 1 cut(s) 32
Bse8I GATNNNNATC 2 cut(s) 1027, 1182
BseAI TCCGGA 1 cut(s) 320
BseDI CCNNGG 3 cut(s) 441, 909, 959
BseGI GGATG 1 cut(s) 577
BseJI GATNNNNATC 2 cut(s) 1027, 1182
BseMII CTCAG 3 cut(s) 147, 1074, 1091
BseNI ACTGG 1 cut(s) 32
BseRI GAGGAG 4 cut(s) 58, 261, 339, 573
BseXI GCAGC 1 cut(s) 427
Bsh1236I CGCG 2 cut(s) 443, 1098
BsiHKCI CYCGRG 1 cut(s) 1010
BsiSI CCGG 6 cut(s) 108, 321, 605, 876, 932, 959
BsmAI GTCTC 2 cut(s) 20, 258
BsoBI CYCGRG 1 cut(s) 1010
Bsp13I TCCGGA 1 cut(s) 320
Bsp143I GATC 8 cut(s) 331, 340, 551, 905, 1022, 1157, 1183, 1202
BspACI CCGC 6 cut(s) 105, 441, 443, 474, 587, 914
BspCNI CTCAG 3 cut(s) 148, 1075, 1090
BspEI TCCGGA 1 cut(s) 320
BspFNI CGCG 2 cut(s) 443, 1098
BspLI GGNNCC 1 cut(s) 124
BspPI GGATC 4 cut(s) 335, 559, 900, 1178
BspQI GCTCTTC 1 cut(s) 222
BsrFI RCCGGY 2 cut(s) 107, 931
BsrI ACTGG 1 cut(s) 32
BssAI RCCGGY 2 cut(s) 107, 931
BssECI CCNNGG 3 cut(s) 441, 909, 959
BssMI GATC 8 cut(s) 331, 340, 551, 905, 1022, 1157, 1183, 1202
BssNI GRCGYC 1 cut(s) 458
Bst4CI ACNGT 7 cut(s) 38, 195, 284, 628, 634, 1050, 1069
Bst6I CTCTTC 3 cut(s) 222, 250, 381
BstACI GRCGYC 1 cut(s) 458
BstDEI CTNAG 3 cut(s) 156, 1077, 1083
BstDSI CCRYGG 2 cut(s) 441, 909
BstF5I GGATG 1 cut(s) 577
BstFNI CGCG 2 cut(s) 443, 1098
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 8 cut(s) 334, 343, 554, 908, 1025, 1160, 1186, 1205
BstMAI GTCTC 2 cut(s) 20, 258
BstMBI GATC 8 cut(s) 331, 340, 551, 905, 1022, 1157, 1183, 1202
BstMWI GCNNNNNNNGC 4 cut(s) 221, 443, 610, 920
BstNSI RCATGY 1 cut(s) 426
BstSCI CCNGG 1 cut(s) 958
BstUI CGCG 2 cut(s) 443, 1098
BstV1I GCAGC 1 cut(s) 427
BstV2I GAAGAC 6 cut(s) 240, 564, 702, 726, 950, 1099
BstX2I RGATCY 5 cut(s) 340, 551, 905, 1157, 1183
BstYI RGATCY 5 cut(s) 340, 551, 905, 1157, 1183
BtgI CCRYGG 2 cut(s) 441, 909
BtrI CACGTC 1 cut(s) 848
BtsCI GGATG 1 cut(s) 577
BtsI GCAGTG 1 cut(s) 1147
BtsIMutI CAGTG 3 cut(s) 43, 639, 1147
CfoI GCGC 1 cut(s) 448
Cfr10I RCCGGY 2 cut(s) 107, 931
Cfr13I GGNCC 1 cut(s) 191
Cfr42I CCGCGG 1 cut(s) 444
CseI GACGC 2 cut(s) 447, 1104
Csp6I GTAC 3 cut(s) 276, 624, 808
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 2 cut(s) 423, 496
CviQI GTAC 3 cut(s) 276, 624, 808
DdeI CTNAG 3 cut(s) 156, 1077, 1083
DpnI GATC 8 cut(s) 333, 342, 553, 907, 1024, 1159, 1185, 1204
DpnII GATC 8 cut(s) 331, 340, 551, 905, 1022, 1157, 1183, 1202
DraI TTTAAA 2 cut(s) 298, 642
DrdI GACNNNNNNGTC 3 cut(s) 248, 876, 1048
DseDI GACNNNNNNGTC 3 cut(s) 248, 876, 1048
Eam1104I CTCTTC 3 cut(s) 222, 250, 381
EarI CTCTTC 3 cut(s) 222, 250, 381
EciI GGCGGA 3 cut(s) 94, 576, 929
Eco47I GGWCC 1 cut(s) 191
Eco57I CTGAAG 2 cut(s) 393, 576
Eco88I CYCGRG 1 cut(s) 1010
FaeI CATG 2 cut(s) 426, 499
FaiI YATR 2 cut(s) 424, 497
FatI CATG 2 cut(s) 422, 495
FauI CCCGC 1 cut(s) 448
FblI GTMKAC 1 cut(s) 1055
Fnu4HI GCNGC 2 cut(s) 416, 444
FokI GGATG 1 cut(s) 564
Fsp4HI GCNGC 2 cut(s) 416, 444
FspBI CTAG 1 cut(s) 1173
GlaI GCGC 1 cut(s) 447
GluI GCNGC 2 cut(s) 416, 444
HapII CCGG 6 cut(s) 108, 321, 605, 876, 932, 959
HgaI GACGC 2 cut(s) 447, 1104
HhaI GCGC 1 cut(s) 448
Hin1I GRCGYC 1 cut(s) 458
Hin1II CATG 2 cut(s) 426, 499
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HincII GTYRAC 1 cut(s) 881
HindII GTYRAC 1 cut(s) 881
HindIII AAGCTT 2 cut(s) 6, 482
HpaII CCGG 6 cut(s) 108, 321, 605, 876, 932, 959
HphI GGTGA 4 cut(s) 122, 130, 640, 1219
Hpy166II GTNNAC 3 cut(s) 881, 1056, 1214
Hpy188III TCNNGA 7 cut(s) 321, 344, 678, 793, 1012, 1089, 1173
Hpy8I GTNNAC 3 cut(s) 881, 1056, 1214
Hpy99I CGWCG 6 cut(s) 185, 188, 852, 1054, 1103, 1192
HpyAV CCTTC 4 cut(s) 68, 182, 811, 1227
HpyCH4III ACNGT 7 cut(s) 38, 195, 284, 628, 634, 1050, 1069
HpyCH4IV ACGT 1 cut(s) 847
HpyCH4V TGCA 2 cut(s) 802, 1152
HpyF10VI GCNNNNNNNGC 4 cut(s) 221, 443, 610, 920
HpyF3I CTNAG 3 cut(s) 156, 1077, 1083
HpySE526I ACGT 1 cut(s) 847
Hsp92I GRCGYC 1 cut(s) 458
Hsp92II CATG 2 cut(s) 426, 499
HspAI GCGC 1 cut(s) 446
Kpn2I TCCGGA 1 cut(s) 320
KspI CCGCGG 1 cut(s) 444
Kzo9I GATC 8 cut(s) 331, 340, 551, 905, 1022, 1157, 1183, 1202
LguI GCTCTTC 1 cut(s) 222
LmnI GCTCC 2 cut(s) 442, 895
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 1 cut(s) 789
MaeI CTAG 1 cut(s) 1173
MaeII ACGT 1 cut(s) 847
MaeIII GTNAC 8 cut(s) 49, 98, 136, 145, 628, 851, 1069, 1194
MalI GATC 8 cut(s) 333, 342, 553, 907, 1024, 1159, 1185, 1204
MboI GATC 8 cut(s) 331, 340, 551, 905, 1022, 1157, 1183, 1202
MflI RGATCY 5 cut(s) 340, 551, 905, 1157, 1183
MluCI AATT 7 cut(s) 299, 462, 544, 637, 663, 863, 1165
MlyI GAGTC 5 cut(s) 258, 260, 1069, 1094, 1097
MmeI TCCRAC 1 cut(s) 232
MroI TCCGGA 1 cut(s) 320
MroXI GAANNNNTTC 2 cut(s) 654, 788
MseI TTAA 7 cut(s) 21, 297, 432, 641, 743, 812, 990
MspA1I CMGCKG 2 cut(s) 443, 983
MspI CCGG 6 cut(s) 108, 321, 605, 876, 932, 959
MspR9I CCNGG 1 cut(s) 960
MvnI CGCG 2 cut(s) 443, 1098
MwoI GCNNNNNNNGC 4 cut(s) 221, 443, 610, 920
NciI CCSGG 1 cut(s) 960
NdeII GATC 8 cut(s) 331, 340, 551, 905, 1022, 1157, 1183, 1202
NlaIII CATG 2 cut(s) 426, 499
NlaIV GGNNCC 1 cut(s) 124
NmuCI GTSAC 2 cut(s) 136, 628
NspI RCATGY 1 cut(s) 426
PciI ACATGT 1 cut(s) 422
PciSI GCTCTTC 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 768
PdmI GAANNNNTTC 2 cut(s) 654, 788
PfeI GAWTC 7 cut(s) 74, 113, 347, 608, 764, 789, 926
PflFI GACNNNGTC 1 cut(s) 1078
PkrI GCNGC 2 cut(s) 417, 445
PleI GAGTC 5 cut(s) 257, 260, 1069, 1093, 1097
PpsI GAGTC 5 cut(s) 257, 260, 1069, 1093, 1097
PscI ACATGT 1 cut(s) 422
PspN4I GGNNCC 1 cut(s) 124
PspPI GGNCC 1 cut(s) 191
PsuI RGATCY 5 cut(s) 340, 551, 905, 1157, 1183
PsyI GACNNNGTC 1 cut(s) 1078
PvuII CAGCTG 1 cut(s) 983
RsaI GTAC 3 cut(s) 277, 625, 809
RsaNI GTAC 3 cut(s) 276, 624, 808
SacII CCGCGG 1 cut(s) 444
SapI GCTCTTC 1 cut(s) 222
SaqAI TTAA 7 cut(s) 21, 297, 432, 641, 743, 812, 990
SatI GCNGC 2 cut(s) 416, 444
Sau3AI GATC 8 cut(s) 331, 340, 551, 905, 1022, 1157, 1183, 1202
Sau96I GGNCC 1 cut(s) 191
ScaI AGTACT 1 cut(s) 809
SchI GAGTC 5 cut(s) 258, 260, 1069, 1094, 1097
ScrFI CCNGG 1 cut(s) 960
SfaNI GCATC 1 cut(s) 789
Sfr303I CCGCGG 1 cut(s) 444
SgrAI CRCCGGYG 2 cut(s) 107, 931
SgrBI CCGCGG 1 cut(s) 444
SinI GGWCC 1 cut(s) 191
SmlI CTYRAG 2 cut(s) 206, 676
SmoI CTYRAG 2 cut(s) 206, 676
Sse9I AATT 7 cut(s) 299, 462, 544, 637, 663, 863, 1165
SsiI CCGC 6 cut(s) 105, 441, 443, 474, 587, 914
SspMI CTAG 1 cut(s) 1173
StyD4I CCNGG 1 cut(s) 958
TaaI ACNGT 7 cut(s) 38, 195, 284, 628, 634, 1050, 1069
TaiI ACGT 1 cut(s) 850
TaqI TCGA 5 cut(s) 201, 334, 354, 564, 692
TasI AATT 7 cut(s) 299, 462, 544, 637, 663, 863, 1165
TatI WGTACW 1 cut(s) 807
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 7 cut(s) 74, 113, 347, 608, 764, 789, 926
Tru1I TTAA 7 cut(s) 21, 297, 432, 641, 743, 812, 990
Tru9I TTAA 7 cut(s) 21, 297, 432, 641, 743, 812, 990
TscAI CASTG 3 cut(s) 43, 639, 1154
TseFI GTSAC 2 cut(s) 136, 628
TseI GCWGC 1 cut(s) 415
Tsp45I GTSAC 2 cut(s) 136, 628
TspDTI ATGAA 4 cut(s) 189, 484, 521, 708
TspGWI ACGGA 3 cut(s) 258, 293, 1176
TspRI CASTG 3 cut(s) 43, 639, 1154
Tth111I GACNNNGTC 1 cut(s) 1078
VpaK11BI GGWCC 1 cut(s) 191
XapI RAATTY 2 cut(s) 299, 637
XbaI TCTAGA 1 cut(s) 1172
XceI RCATGY 1 cut(s) 426
XmiI GTMKAC 1 cut(s) 1055
XmnI GAANNNNTTC 2 cut(s) 654, 788
XspI CTAG 1 cut(s) 1173
ZrmI AGTACT 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.