Rh7DG158400

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0015380
Physical Location & Seq
Forward (+)
13807394 .. 13808858
1465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG158400.1

Sequence Viewer

Length: 1287 bp
ATGGAAGCTTTTAGCCTGCTCAAGTATTGGCGAGGTGGCGGCCTTGTTGCTTCCTCCGCTCCTCCTAATGGCCCTAACAATGTCACCTCCGCCGCCGCCACCATCCTCACCGCCGTCGCTGAGAACAGCGACGAGAGTCATGAAACTGACGACGACGACGACGACGGACCTTTCTTTGACTTGGAGTTTGCCGTCCCCGACGAGGCCGAAGCCCACGAAGCCTCTGGAAGCAGCGACAGCGCCACCAGCGGCGAAGAGAGTGAGGTGGAAGTGCACCAAGATGGGGAAGATGATGAGTCCGATGATGATGATGATGATGACGTGGACGTGGACGGTGAGGAGGGTGAGTTTAACTTCACTGTGTCTTCCGGGTCGAGCAATGACGGCACCGATCCGAATCTGTCGATGTCTCCCTCTGACGATTTGTTTTTCAAGGGTAAGCTGGTGCCGATTGAGCCTTCCTCCATTGTGTTCAACCCGTCCGAGCCGAACTCCAAGGCTCAATTCGCAGGGTCGCTGCTCAAGTCCGCCACCAAGTTCCGGGTCTTCATGTCGGGTCTCAAGAAATCCAAGTCCAATTCCAACGGCCCGGAAAACAAAACGGAACCTACAAACGGATCCGTACAAGAACAACATGAAAAACAAGAGAATAAGCAACAGAAGCAGGGGAAGCTTTTCACGGTGAAGTTGAAGGTGGAGGAAGTTCCCATTGTGTCCTTGTTCACCAGAGACAACAGCTCCAGAATCTCCGCCAAGACCCAGAACCAGAAAACCCAGAGTTCCGAAGAAGCTGCTTCTGAGGAGAGGCGCTTTTCGAAAGAGGTAATGCAGAAGTACTTGAAAATGGTGAAGCCTCTCTACGTCCGCGTTTCGAAACGCTACGGCGAGAAGACGAGGCTCTCCGGTCAACTCAGCGTAAGCAGCCCGCAGCCGGCGGAGAAGAGCCAGGCGGAGGCTGAAGTTTCGGAACCGGCGGCGAGCAATGTGAAGAGTACTTTGAAGCAGGGGAATATTCCGGCAGGTTTGAGAGTTGTTTGCAAGCACTTGGGGAAGAGCCGCTCGGCTTCTTCGGCTGTAGCCGCGGCTCCACCGGGAACTGTGCTGTCACAGAGGAGAGATGATTCGCTACTGCAGCAGCAGGATGGGATCCAGAGCGCCATTCTCCATTGCAAGCGGTCATTCAATGCTTCCAGAGATTCCGATTCTACTCCGCTATCCCGGTCGGTGAGCGATTCCTGCAACGAGAAGGCGTCACGAAAACCGTCGGATCAAGGCGGAGAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

428

Amino Acids

46.1

Weight (kDa)

4.78

Isoelectric Point (pI)

54.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64080
fragaria_vesca FvH4_5g03470
malus_domestica MD06G1094800.v1.1 MD14G1115100.v1.1
prunus_persica Prupe.5G110000_v2.0.a1
pyrus_communis pycom06g09150
rosa_chinensis RchiOBHm_Chr7g0196191
rosa_laevigata RLG00000004053
rosa_multiflora Rmu_sc0007292.1_g000008
rosa_roxburghii Rroxscaffold_3G00259680
rosa_rugosa Rorug07G0032000
rosa_samantha Rh7AG157400 Rh7BG159100 Rh7CG163100 Rh7DG158400
rosa_wichuraiana Rw7G013460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1010
AccB1I GGYRCC 2 cut(s) 386, 445
AccBSI CCGCTC 2 cut(s) 59, 1059
AccII CGCG 2 cut(s) 867, 1082
AclWI GGATC 6 cut(s) 386, 612, 625, 1141, 1154, 1275
AcuI CTGAAG 1 cut(s) 978
AcyI GRCGYC 1 cut(s) 1250
AfaI GTAC 3 cut(s) 624, 836, 994
AfiI CCNNNNNNNGG 7 cut(s) 68, 202, 283, 540, 614, 931, 952
AgsI TTSAA 6 cut(s) 433, 475, 691, 841, 1000, 1183
AjiI CACGTC 2 cut(s) 322, 328
AjnI CCWGG 1 cut(s) 945
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AluBI AGCT 5 cut(s) 8, 442, 673, 738, 791
AluI AGCT 5 cut(s) 8, 442, 673, 738, 791
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 3 cut(s) 414, 563, 723
Alw44I GTGCAC 1 cut(s) 272
AlwI GGATC 6 cut(s) 386, 612, 625, 1141, 1154, 1275
AoxI GGCC 4 cut(s) 40, 70, 204, 586
ApaLI GTGCAC 1 cut(s) 272
ApeKI GCWGC 7 cut(s) 231, 517, 791, 921, 928, 1132, 1135
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
Asp700I GAANNNNTTC 1 cut(s) 674
AspLEI GCGC 3 cut(s) 242, 810, 1157
AspS9I GGNCC 3 cut(s) 71, 167, 587
AsuC2I CCSGG 5 cut(s) 370, 542, 590, 1092, 1219
AsuHPI GGTGA 8 cut(s) 76, 100, 347, 356, 694, 715, 859, 1237
AsuII TTCGAA 2 cut(s) 815, 872
AvaII GGWCC 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 276
BamHI GGATCC 2 cut(s) 617, 1146
BanI GGYRCC 2 cut(s) 386, 445
BarI GAAGNNNNNNTAC 2 cut(s) 842, 874
BbsI GAAGAC 3 cut(s) 357, 538, 896
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 7 cut(s) 243, 504, 778, 933, 940, 1144, 1147
BccI CCATC 3 cut(s) 110, 275, 1136
BceAI ACGGC 5 cut(s) 98, 176, 400, 601, 898
BcgI CGANNNNNNTGC 2 cut(s) 773, 807
BciT130I CCWGG 1 cut(s) 947
BcnI CCSGG 5 cut(s) 370, 542, 590, 1092, 1219
BcoDI GTCTC 3 cut(s) 414, 563, 723
BfmI CTRYAG 2 cut(s) 1074, 1130
BfoI RGCGCY 3 cut(s) 243, 811, 1158
BfuAI ACCTGC 1 cut(s) 1010
BmcAI AGTACT 2 cut(s) 836, 994
Bme1390I CCNGG 6 cut(s) 370, 542, 590, 947, 1092, 1219
Bme18I GGWCC 1 cut(s) 167
BmgBI CACGTC 2 cut(s) 322, 328
BmgT120I GGNCC 3 cut(s) 71, 167, 587
BmiI GGNNCC 7 cut(s) 388, 447, 606, 619, 969, 1086, 1148
BmrFI CCNGG 6 cut(s) 370, 542, 590, 947, 1092, 1219
BoxI GACNNNNGTC 1 cut(s) 135
BpiI GAAGAC 3 cut(s) 357, 538, 896
BplI GAGNNNNNCTC 4 cut(s) 446, 478, 476, 508
BpmI CTGGAG 1 cut(s) 724
Bpu14I TTCGAA 2 cut(s) 815, 872
BpuEI CTTGAG 3 cut(s) 5, 506, 545
BpuMI CCSGG 5 cut(s) 370, 542, 590, 1092, 1219
BsaBI GATNNNNATC 1 cut(s) 396
BsaHI GRCGYC 1 cut(s) 1250
BsaI GGTCTC 1 cut(s) 563
BsaJI CCNNGG 2 cut(s) 495, 1080
BsaWI WCCGGW 1 cut(s) 902
BsaXI ACNNNNNCTCC 4 cut(s) 332, 362, 722, 752
Bsc4I CCNNNNNNNGG 7 cut(s) 68, 202, 283, 540, 614, 931, 952
Bse118I RCCGGY 2 cut(s) 931, 970
Bse3DI GCAATG 3 cut(s) 385, 988, 1165
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 1 cut(s) 947
BseDI CCNNGG 2 cut(s) 495, 1080
BseGI GGATG 2 cut(s) 102, 1147
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 7 cut(s) 68, 202, 283, 540, 614, 931, 952
BseMI GCAATG 3 cut(s) 385, 988, 1165
BseMII CTCAG 3 cut(s) 111, 789, 925
BseRI GAGGAG 4 cut(s) 51, 353, 815, 1126
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 7 cut(s) 243, 504, 778, 933, 940, 1144, 1147
Bsh1236I CGCG 2 cut(s) 867, 1082
Bsh1285I CGRYCG 1 cut(s) 1223
BshFI GGCC 4 cut(s) 42, 72, 206, 588
BshNI GGYRCC 2 cut(s) 386, 445
BsiEI CGRYCG 1 cut(s) 1223
BsiHKAI GWGCWC 1 cut(s) 276
BsiSI CCGG 9 cut(s) 369, 541, 590, 903, 932, 971, 1016, 1091, 1219
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 7 cut(s) 68, 202, 283, 540, 614, 931, 952
BsmAI GTCTC 3 cut(s) 414, 563, 723
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 4 cut(s) 42, 72, 206, 588
Bso31I GGTCTC 1 cut(s) 563
Bsp119I TTCGAA 2 cut(s) 815, 872
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 4 cut(s) 391, 617, 1146, 1267
BspANI GGCC 4 cut(s) 42, 72, 206, 588
BspCNI CTCAG 3 cut(s) 112, 790, 924
BspFNI CGCG 2 cut(s) 867, 1082
BspHI TCATGA 1 cut(s) 139
BspLI GGNNCC 7 cut(s) 388, 447, 606, 619, 969, 1086, 1148
BspMAI CTGCAG 1 cut(s) 1134
BspMI ACCTGC 1 cut(s) 1010
BspPI GGATC 6 cut(s) 386, 612, 625, 1141, 1154, 1275
BspQI GCTCTTC 2 cut(s) 935, 1046
BspT104I TTCGAA 2 cut(s) 815, 872
BspT107I GGYRCC 2 cut(s) 386, 445
BspTNI GGTCTC 1 cut(s) 563
BsrBI CCGCTC 2 cut(s) 59, 1059
BsrDI GCAATG 3 cut(s) 385, 988, 1165
BsrFI RCCGGY 2 cut(s) 931, 970
BssAI RCCGGY 2 cut(s) 931, 970
BssECI CCNNGG 2 cut(s) 495, 1080
BssMI GATC 4 cut(s) 391, 617, 1146, 1267
BssNI GRCGYC 1 cut(s) 1250
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 947
Bst4CI ACNGT 5 cut(s) 335, 361, 682, 1099, 1263
Bst6I CTCTTC 4 cut(s) 249, 935, 983, 1046
BstACI GRCGYC 1 cut(s) 1250
BstBI TTCGAA 2 cut(s) 815, 872
BstC8I GCNNGC 6 cut(s) 17, 926, 933, 979, 1040, 1172
BstDEI CTNAG 3 cut(s) 120, 798, 911
BstDSI CCRYGG 1 cut(s) 1080
BstF5I GGATG 2 cut(s) 102, 1147
BstFNI CGCG 2 cut(s) 867, 1082
BstH2I RGCGCY 3 cut(s) 243, 811, 1158
BstHHI GCGC 3 cut(s) 242, 810, 1157
BstKTI GATC 4 cut(s) 394, 620, 1149, 1270
BstMAI GTCTC 3 cut(s) 414, 563, 723
BstMBI GATC 4 cut(s) 391, 617, 1146, 1267
BstMCI CGRYCG 1 cut(s) 1223
BstNI CCWGG 1 cut(s) 947
BstPAI GACNNNNGTC 1 cut(s) 135
BstSCI CCNGG 6 cut(s) 368, 540, 588, 945, 1090, 1217
BstSFI CTRYAG 2 cut(s) 1074, 1130
BstSLI GKGCMC 1 cut(s) 276
BstUI CGCG 2 cut(s) 867, 1082
BstV1I GCAGC 7 cut(s) 243, 504, 778, 933, 940, 1144, 1147
BstV2I GAAGAC 3 cut(s) 357, 538, 896
BstX2I RGATCY 2 cut(s) 617, 1146
BstYI RGATCY 2 cut(s) 617, 1146
BsuRI GGCC 4 cut(s) 42, 72, 206, 588
BtgI CCRYGG 1 cut(s) 1080
BtrI CACGTC 2 cut(s) 322, 328
BtsCI GGATG 2 cut(s) 102, 1147
BtsIMutI CAGTG 1 cut(s) 357
BveI ACCTGC 1 cut(s) 1010
Cac8I GCNNGC 6 cut(s) 17, 926, 933, 979, 1040, 1172
CciI TCATGA 1 cut(s) 139
CfoI GCGC 3 cut(s) 242, 810, 1157
Cfr10I RCCGGY 2 cut(s) 931, 970
Cfr13I GGNCC 3 cut(s) 71, 167, 587
Cfr42I CCGCGG 1 cut(s) 1083
CseI GACGC 1 cut(s) 1239
Csp6I GTAC 3 cut(s) 623, 835, 993
CviAII CATG 3 cut(s) 140, 550, 635
CviQI GTAC 3 cut(s) 623, 835, 993
DdeI CTNAG 3 cut(s) 120, 798, 911
DpnI GATC 4 cut(s) 393, 619, 1148, 1269
DpnII GATC 4 cut(s) 391, 617, 1146, 1267
Eam1104I CTCTTC 4 cut(s) 249, 935, 983, 1046
EarI CTCTTC 4 cut(s) 249, 935, 983, 1046
EciI GGCGGA 5 cut(s) 79, 517, 739, 950, 965
Eco130I CCWWGG 1 cut(s) 495
Eco31I GGTCTC 1 cut(s) 563
Eco47I GGWCC 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 978
EcoRII CCWGG 1 cut(s) 945
EcoT14I CCWWGG 1 cut(s) 495
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 3 cut(s) 143, 553, 638
FaiI YATR 3 cut(s) 141, 551, 636
FaqI GGGAC 1 cut(s) 179
FatI CATG 3 cut(s) 139, 549, 634
FauI CCCGC 1 cut(s) 933
FokI GGATG 2 cut(s) 89, 1154
GlaI GCGC 3 cut(s) 241, 809, 1156
GsuI CTGGAG 1 cut(s) 724
HaeII RGCGCY 3 cut(s) 243, 811, 1158
HaeIII GGCC 4 cut(s) 42, 72, 206, 588
HapII CCGG 9 cut(s) 369, 541, 590, 903, 932, 971, 1016, 1091, 1219
HgaI GACGC 1 cut(s) 1239
HhaI GCGC 3 cut(s) 242, 810, 1157
Hin1I GRCGYC 1 cut(s) 1250
Hin1II CATG 3 cut(s) 143, 553, 638
Hin6I GCGC 3 cut(s) 240, 808, 1155
HinP1I GCGC 3 cut(s) 240, 808, 1155
HincII GTYRAC 1 cut(s) 908
HindII GTYRAC 1 cut(s) 908
HindIII AAGCTT 2 cut(s) 6, 671
HinfI GANTC 8 cut(s) 136, 296, 397, 744, 1121, 1196, 1202, 1232
HpaII CCGG 9 cut(s) 369, 541, 590, 903, 932, 971, 1016, 1091, 1219
HphI GGTGA 8 cut(s) 76, 100, 347, 356, 694, 715, 859, 1237
Hpy166II GTNNAC 5 cut(s) 274, 325, 331, 723, 908
Hpy188I TCNGA 9 cut(s) 301, 396, 418, 484, 784, 799, 967, 1201, 1267
Hpy188III TCNNGA 7 cut(s) 140, 225, 562, 741, 1150, 1191, 1254
Hpy8I GTNNAC 5 cut(s) 274, 325, 331, 723, 908
Hpy99I CGWCG 9 cut(s) 119, 134, 155, 158, 161, 164, 167, 203, 1267
HpyAV CCTTC 3 cut(s) 468, 685, 1240
HpyCH4III ACNGT 5 cut(s) 335, 361, 682, 1099, 1263
HpyCH4IV ACGT 3 cut(s) 321, 327, 861
HpyCH4V TGCA 6 cut(s) 274, 829, 1038, 1132, 1170, 1239
HpyF3I CTNAG 3 cut(s) 120, 798, 911
HpySE526I ACGT 3 cut(s) 321, 327, 861
Hsp92I GRCGYC 1 cut(s) 1250
Hsp92II CATG 3 cut(s) 143, 553, 638
HspAI GCGC 3 cut(s) 240, 808, 1155
KroI GCCGGC 1 cut(s) 931
KroNI GCCGGC 1 cut(s) 933
KspI CCGCGG 1 cut(s) 1083
Kzo9I GATC 4 cut(s) 391, 617, 1146, 1267
LguI GCTCTTC 2 cut(s) 935, 1046
LmnI GCTCC 3 cut(s) 64, 743, 1090
Lsp1109I GCAGC 7 cut(s) 243, 504, 778, 933, 940, 1144, 1147
MaeII ACGT 3 cut(s) 321, 327, 861
MaeIII GTNAC 3 cut(s) 82, 1104, 1251
MalI GATC 4 cut(s) 393, 619, 1148, 1269
MbiI CCGCTC 2 cut(s) 59, 1059
MboI GATC 4 cut(s) 391, 617, 1146, 1267
MflI RGATCY 2 cut(s) 617, 1146
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 2 cut(s) 503, 577
MlyI GAGTC 2 cut(s) 145, 305
MmeI TCCRAC 2 cut(s) 606, 1245
MroNI GCCGGC 1 cut(s) 931
MroXI GAANNNNTTC 1 cut(s) 674
MseI TTAA 1 cut(s) 351
MslI CAYNNNNRTG 1 cut(s) 279
MspA1I CMGCKG 2 cut(s) 249, 1082
MspI CCGG 9 cut(s) 369, 541, 590, 903, 932, 971, 1016, 1091, 1219
MspR9I CCNGG 6 cut(s) 370, 542, 590, 947, 1092, 1219
MvaI CCWGG 1 cut(s) 947
MvnI CGCG 2 cut(s) 867, 1082
NaeI GCCGGC 1 cut(s) 933
NciI CCSGG 5 cut(s) 370, 542, 590, 1092, 1219
NdeII GATC 4 cut(s) 391, 617, 1146, 1267
NgoMIV GCCGGC 1 cut(s) 931
NlaIII CATG 3 cut(s) 143, 553, 638
NlaIV GGNNCC 7 cut(s) 388, 447, 606, 619, 969, 1086, 1148
NmeAIII GCCGAG 1 cut(s) 1040
NmuCI GTSAC 3 cut(s) 82, 1104, 1251
NspV TTCGAA 2 cut(s) 815, 872
PagI TCATGA 1 cut(s) 139
PciSI GCTCTTC 2 cut(s) 935, 1046
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 159
PdiI GCCGGC 1 cut(s) 933
PdmI GAANNNNTTC 1 cut(s) 674
PfeI GAWTC 6 cut(s) 397, 744, 1121, 1196, 1202, 1232
PleI GAGTC 2 cut(s) 144, 304
PpsI GAGTC 2 cut(s) 144, 304
PshAI GACNNNNGTC 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 945
PspGI CCWGG 1 cut(s) 945
PspN4I GGNNCC 7 cut(s) 388, 447, 606, 619, 969, 1086, 1148
PspPI GGNCC 3 cut(s) 71, 167, 587
PstI CTGCAG 1 cut(s) 1134
PsuI RGATCY 2 cut(s) 617, 1146
RsaI GTAC 3 cut(s) 624, 836, 994
RsaNI GTAC 3 cut(s) 623, 835, 993
RseI CAYNNNNRTG 1 cut(s) 279
SacII CCGCGG 1 cut(s) 1083
SapI GCTCTTC 2 cut(s) 935, 1046
SaqAI TTAA 1 cut(s) 351
Sau3AI GATC 4 cut(s) 391, 617, 1146, 1267
Sau96I GGNCC 3 cut(s) 71, 167, 587
ScaI AGTACT 2 cut(s) 836, 994
SchI GAGTC 2 cut(s) 145, 305
ScrFI CCNGG 6 cut(s) 370, 542, 590, 947, 1092, 1219
SduI GDGCHC 1 cut(s) 276
SfcI CTRYAG 2 cut(s) 1074, 1130
Sfr303I CCGCGG 1 cut(s) 1083
SfuI TTCGAA 2 cut(s) 815, 872
SgrBI CCGCGG 1 cut(s) 1083
SinI GGWCC 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 279
SmlI CTYRAG 3 cut(s) 20, 521, 560
SmoI CTYRAG 3 cut(s) 20, 521, 560
Sse9I AATT 2 cut(s) 503, 577
SspI AATATT 1 cut(s) 1012
StyD4I CCNGG 6 cut(s) 368, 540, 588, 945, 1090, 1217
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 5 cut(s) 335, 361, 682, 1099, 1263
TaiI ACGT 3 cut(s) 324, 330, 864
TaqI TCGA 4 cut(s) 374, 404, 815, 872
TasI AATT 2 cut(s) 503, 577
TatI WGTACW 2 cut(s) 834, 992
TauI GCSGC 8 cut(s) 42, 95, 98, 252, 977, 1059, 1082, 1085
TfiI GAWTC 6 cut(s) 397, 744, 1121, 1196, 1202, 1232
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 3 cut(s) 82, 1104, 1251
TseI GCWGC 7 cut(s) 231, 517, 791, 921, 928, 1132, 1135
Tsp45I GTSAC 3 cut(s) 82, 1104, 1251
TspDTI ATGAA 3 cut(s) 156, 538, 651
TspGWI ACGGA 4 cut(s) 180, 610, 617, 630
TspRI CASTG 1 cut(s) 364
VneI GTGCAC 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 674
ZrmI AGTACT 2 cut(s) 836, 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.