Rh7CG163100

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0015380
Physical Location & Seq
Forward (+)
13479294 .. 13481447
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG163100.1

Sequence Viewer

Length: 1278 bp
ATGGAAGCTTTTAGCCTGCTCAAGTATTGGCGAGGTGGCGGCCTTGTTGCTTCCTCCGCTCCTCCTAATGGCCCTAACAATGTCACCTCCGCCGCCGCCGCCACCATCCTCACCGCCGTCGCTGAGAACAGCGACGAGAGTCATGAAACTGACGACGACGACGACGGACCTTTCTTTGACTTGGAGTTTGCCGTCCCTGACGAGGCCGAAGCCCACGAAGCCTCTGGAAGCAGCGACAGCGCCACCAGCGGCGAAGAGAGTGAGGTGGAAGTGCACCAAGATGGGGAAGATGATGAGTCCGATGATGATGATGACGTGGACGTGGACGTGGACGGTGAGTTTAACTTCACTGTGTCTTCCGGGTCGAGCAATGACGGCACCGATCCGAATCTGTCGATGTCTCCCTCTGACGATTTGTTCTTCAAGGGTAAGCTGGTGCCGATTGAGCCTTCCTCCATTGTGTTCAACCCGTCCGAGCCGAACTCCAAGGCTCAATTCGCAGGGTCGCTGCTCAAGTCCGCCACCAAGTTCCGGGTCTTCATGTCGGGTCTCAAGAAATCCAAGTCCAATTCCAACGGCCCGGAAAACAAAACGGAACCTACAAACGGATCCGTACAAGAACAACATGAAAAACAAGAGAATAAGCAACAGAAGCAGGGGAAGCTTTTCACGGTGAAGTTGAAGGTGGAGGAAGTTCCCATTGTGTCCTTGTTCACCAGAGACAACAGCTCCAGAATCTCCACCAAGACCCAGAACCAGAAAACCCAGAGTTCCGAAGAAGCTGCTTCTGAGGAGAAGCGCTTTTCGAAAGAGGTAATGCAGAAGTACTTGAAAATGGTGAAGCCTCTCTACGTCCGCGTTTCGAAACGCTACGGCGAGAAGACGAGGCTCTCCGGTCAACTCAGCGTAAGCAGCCCGCAGCCGGCTGAGAAGAGCCAGGTGGAGGCTGAAGTTTCGGAACCGGCGGCGAGCAATGTGAAGAGTACTTTGAAGCAGGGGAATATTCCGGCGGGTTTGAGAGTTGTTTGCAAGCACTTGGGGAAGAGCCGCTCGGCTTCTTCGGCTGTAGCCGCGGCTCCACCGGGAACTGTGCTGTCACAGAGGAGAGATGATTCGCTGCTGCAGCAGCAGGATGGGATCCAGAGCGCCATTCTCCATTGCAAGCGGTCATTCAATGCTTCCAGAGATTCCGATTCTACTCCGCTATCCCGGTCGGTGAGCGATTCCTGCAACGAGAAGGCGTCACGAAAACCATCGGATCAAGGCGGAGAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

425

Amino Acids

45.75

Weight (kDa)

4.9

Isoelectric Point (pI)

52.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64080
fragaria_vesca FvH4_5g03470
malus_domestica MD06G1094800.v1.1 MD14G1115100.v1.1
prunus_persica Prupe.5G110000_v2.0.a1
pyrus_communis pycom06g09150
rosa_chinensis RchiOBHm_Chr7g0196191
rosa_laevigata RLG00000004053
rosa_multiflora Rmu_sc0007292.1_g000008
rosa_roxburghii Rroxscaffold_3G00259680
rosa_rugosa Rorug07G0032000
rosa_samantha Rh7AG157400 Rh7BG159100 Rh7CG163100 Rh7DG158400
rosa_wichuraiana Rw7G013460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 377, 436
AccBSI CCGCTC 2 cut(s) 59, 1050
AccII CGCG 2 cut(s) 858, 1073
AclWI GGATC 6 cut(s) 377, 603, 616, 1132, 1145, 1266
AcuI CTGAAG 1 cut(s) 969
AcyI GRCGYC 1 cut(s) 1241
AfaI GTAC 3 cut(s) 615, 827, 985
AfeI AGCGCT 1 cut(s) 800
AfiI CCNNNNNNNGG 7 cut(s) 68, 202, 283, 531, 605, 922, 943
AgsI TTSAA 6 cut(s) 424, 466, 682, 832, 991, 1174
AjiI CACGTC 3 cut(s) 316, 322, 328
AjnI CCWGG 1 cut(s) 936
AjuI GAANNNNNNNTTGG 2 cut(s) 479, 511
AluBI AGCT 5 cut(s) 8, 433, 664, 729, 782
AluI AGCT 5 cut(s) 8, 433, 664, 729, 782
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 3 cut(s) 405, 554, 714
Alw44I GTGCAC 1 cut(s) 272
AlwI GGATC 6 cut(s) 377, 603, 616, 1132, 1145, 1266
Aor51HI AGCGCT 1 cut(s) 800
AoxI GGCC 4 cut(s) 40, 70, 204, 577
ApaLI GTGCAC 1 cut(s) 272
ApeKI GCWGC 9 cut(s) 231, 508, 782, 912, 919, 1117, 1120, 1123, 1126
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
Asp700I GAANNNNTTC 1 cut(s) 665
AspLEI GCGC 3 cut(s) 242, 801, 1148
AspS9I GGNCC 3 cut(s) 71, 167, 578
AsuC2I CCSGG 5 cut(s) 361, 533, 581, 1083, 1210
AsuHPI GGTGA 7 cut(s) 76, 103, 347, 685, 706, 850, 1228
AsuII TTCGAA 2 cut(s) 806, 863
AvaII GGWCC 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 276
BamHI GGATCC 2 cut(s) 608, 1137
BanI GGYRCC 2 cut(s) 377, 436
BarI GAAGNNNNNNTAC 2 cut(s) 833, 865
BbsI GAAGAC 3 cut(s) 348, 529, 887
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 9 cut(s) 243, 495, 769, 924, 931, 1104, 1107, 1135, 1138
BccI CCATC 4 cut(s) 113, 275, 1127, 1261
BceAI ACGGC 5 cut(s) 101, 176, 391, 592, 889
BcgI CGANNNNNNTGC 2 cut(s) 764, 798
BciT130I CCWGG 1 cut(s) 938
BcnI CCSGG 5 cut(s) 361, 533, 581, 1083, 1210
BcoDI GTCTC 3 cut(s) 405, 554, 714
BfmI CTRYAG 2 cut(s) 1065, 1121
BfoI RGCGCY 3 cut(s) 243, 802, 1149
BmcAI AGTACT 2 cut(s) 827, 985
Bme1390I CCNGG 6 cut(s) 361, 533, 581, 938, 1083, 1210
Bme18I GGWCC 1 cut(s) 167
BmgBI CACGTC 3 cut(s) 316, 322, 328
BmgT120I GGNCC 3 cut(s) 71, 167, 578
BmiI GGNNCC 7 cut(s) 379, 438, 597, 610, 960, 1077, 1139
BmrFI CCNGG 6 cut(s) 361, 533, 581, 938, 1083, 1210
BoxI GACNNNNGTC 1 cut(s) 138
BpiI GAAGAC 3 cut(s) 348, 529, 887
BplI GAGNNNNNCTC 4 cut(s) 437, 469, 467, 499
BpmI CTGGAG 1 cut(s) 715
Bpu14I TTCGAA 2 cut(s) 806, 863
BpuEI CTTGAG 3 cut(s) 5, 497, 536
BpuMI CCSGG 5 cut(s) 361, 533, 581, 1083, 1210
BsaBI GATNNNNATC 1 cut(s) 387
BsaHI GRCGYC 1 cut(s) 1241
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 2 cut(s) 486, 1071
BsaWI WCCGGW 1 cut(s) 893
BsaXI ACNNNNNCTCC 2 cut(s) 713, 743
Bsc4I CCNNNNNNNGG 7 cut(s) 68, 202, 283, 531, 605, 922, 943
Bse118I RCCGGY 2 cut(s) 922, 961
Bse3DI GCAATG 3 cut(s) 376, 979, 1156
Bse8I GATNNNNATC 1 cut(s) 387
BseBI CCWGG 1 cut(s) 938
BseDI CCNNGG 2 cut(s) 486, 1071
BseGI GGATG 2 cut(s) 105, 1138
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 7 cut(s) 68, 202, 283, 531, 605, 922, 943
BseMI GCAATG 3 cut(s) 376, 979, 1156
BseMII CTCAG 4 cut(s) 114, 780, 916, 918
BseRI GAGGAG 3 cut(s) 51, 806, 1117
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 9 cut(s) 243, 495, 769, 924, 931, 1104, 1107, 1135, 1138
Bsh1236I CGCG 2 cut(s) 858, 1073
Bsh1285I CGRYCG 1 cut(s) 1214
BshFI GGCC 4 cut(s) 42, 72, 206, 579
BshNI GGYRCC 2 cut(s) 377, 436
BsiEI CGRYCG 1 cut(s) 1214
BsiHKAI GWGCWC 1 cut(s) 276
BsiSI CCGG 9 cut(s) 360, 532, 581, 894, 923, 962, 1007, 1082, 1210
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 7 cut(s) 68, 202, 283, 531, 605, 922, 943
BsmAI GTCTC 3 cut(s) 405, 554, 714
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 4 cut(s) 42, 72, 206, 579
Bso31I GGTCTC 1 cut(s) 554
Bsp119I TTCGAA 2 cut(s) 806, 863
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 4 cut(s) 382, 608, 1137, 1258
BspANI GGCC 4 cut(s) 42, 72, 206, 579
BspCNI CTCAG 4 cut(s) 115, 781, 915, 919
BspFNI CGCG 2 cut(s) 858, 1073
BspHI TCATGA 1 cut(s) 142
BspLI GGNNCC 7 cut(s) 379, 438, 597, 610, 960, 1077, 1139
BspMAI CTGCAG 1 cut(s) 1125
BspPI GGATC 6 cut(s) 377, 603, 616, 1132, 1145, 1266
BspQI GCTCTTC 2 cut(s) 926, 1037
BspT104I TTCGAA 2 cut(s) 806, 863
BspT107I GGYRCC 2 cut(s) 377, 436
BspTNI GGTCTC 1 cut(s) 554
BsrBI CCGCTC 2 cut(s) 59, 1050
BsrDI GCAATG 3 cut(s) 376, 979, 1156
BsrFI RCCGGY 2 cut(s) 922, 961
BssAI RCCGGY 2 cut(s) 922, 961
BssECI CCNNGG 2 cut(s) 486, 1071
BssMI GATC 4 cut(s) 382, 608, 1137, 1258
BssNI GRCGYC 1 cut(s) 1241
BssT1I CCWWGG 1 cut(s) 486
Bst2UI CCWGG 1 cut(s) 938
Bst4CI ACNGT 4 cut(s) 335, 352, 673, 1090
Bst6I CTCTTC 4 cut(s) 249, 926, 974, 1037
BstACI GRCGYC 1 cut(s) 1241
BstBI TTCGAA 2 cut(s) 806, 863
BstC8I GCNNGC 6 cut(s) 17, 917, 924, 970, 1031, 1163
BstDEI CTNAG 4 cut(s) 123, 789, 902, 927
BstDSI CCRYGG 1 cut(s) 1071
BstF5I GGATG 2 cut(s) 105, 1138
BstFNI CGCG 2 cut(s) 858, 1073
BstH2I RGCGCY 3 cut(s) 243, 802, 1149
BstHHI GCGC 3 cut(s) 242, 801, 1148
BstKTI GATC 4 cut(s) 385, 611, 1140, 1261
BstMAI GTCTC 3 cut(s) 405, 554, 714
BstMBI GATC 4 cut(s) 382, 608, 1137, 1258
BstMCI CGRYCG 1 cut(s) 1214
BstNI CCWGG 1 cut(s) 938
BstPAI GACNNNNGTC 1 cut(s) 138
BstSCI CCNGG 6 cut(s) 359, 531, 579, 936, 1081, 1208
BstSFI CTRYAG 2 cut(s) 1065, 1121
BstSLI GKGCMC 1 cut(s) 276
BstUI CGCG 2 cut(s) 858, 1073
BstV1I GCAGC 9 cut(s) 243, 495, 769, 924, 931, 1104, 1107, 1135, 1138
BstV2I GAAGAC 3 cut(s) 348, 529, 887
BstX2I RGATCY 2 cut(s) 608, 1137
BstYI RGATCY 2 cut(s) 608, 1137
BsuRI GGCC 4 cut(s) 42, 72, 206, 579
BtgI CCRYGG 1 cut(s) 1071
BtrI CACGTC 3 cut(s) 316, 322, 328
BtsCI GGATG 2 cut(s) 105, 1138
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 6 cut(s) 17, 917, 924, 970, 1031, 1163
CciI TCATGA 1 cut(s) 142
CfoI GCGC 3 cut(s) 242, 801, 1148
Cfr10I RCCGGY 2 cut(s) 922, 961
Cfr13I GGNCC 3 cut(s) 71, 167, 578
Cfr42I CCGCGG 1 cut(s) 1074
CseI GACGC 1 cut(s) 1230
Csp6I GTAC 3 cut(s) 614, 826, 984
CviAII CATG 3 cut(s) 143, 541, 626
CviQI GTAC 3 cut(s) 614, 826, 984
DdeI CTNAG 4 cut(s) 123, 789, 902, 927
DpnI GATC 4 cut(s) 384, 610, 1139, 1260
DpnII GATC 4 cut(s) 382, 608, 1137, 1258
Eam1104I CTCTTC 4 cut(s) 249, 926, 974, 1037
EarI CTCTTC 4 cut(s) 249, 926, 974, 1037
EciI GGCGGA 2 cut(s) 79, 508
Eco130I CCWWGG 1 cut(s) 486
Eco31I GGTCTC 1 cut(s) 554
Eco47I GGWCC 1 cut(s) 167
Eco47III AGCGCT 1 cut(s) 800
Eco57I CTGAAG 1 cut(s) 969
EcoRII CCWGG 1 cut(s) 936
EcoT14I CCWWGG 1 cut(s) 486
ErhI CCWWGG 1 cut(s) 486
FaeI CATG 3 cut(s) 146, 544, 629
FaiI YATR 3 cut(s) 144, 542, 627
FaqI GGGAC 1 cut(s) 179
FatI CATG 3 cut(s) 142, 540, 625
FauI CCCGC 2 cut(s) 924, 1003
FokI GGATG 2 cut(s) 92, 1145
GlaI GCGC 3 cut(s) 241, 800, 1147
GsuI CTGGAG 1 cut(s) 715
HaeII RGCGCY 3 cut(s) 243, 802, 1149
HaeIII GGCC 4 cut(s) 42, 72, 206, 579
HapII CCGG 9 cut(s) 360, 532, 581, 894, 923, 962, 1007, 1082, 1210
HgaI GACGC 1 cut(s) 1230
HhaI GCGC 3 cut(s) 242, 801, 1148
Hin1I GRCGYC 1 cut(s) 1241
Hin1II CATG 3 cut(s) 146, 544, 629
Hin6I GCGC 3 cut(s) 240, 799, 1146
HinP1I GCGC 3 cut(s) 240, 799, 1146
HincII GTYRAC 1 cut(s) 899
HindII GTYRAC 1 cut(s) 899
HindIII AAGCTT 2 cut(s) 6, 662
HinfI GANTC 8 cut(s) 139, 296, 388, 735, 1112, 1187, 1193, 1223
HpaII CCGG 9 cut(s) 360, 532, 581, 894, 923, 962, 1007, 1082, 1210
HphI GGTGA 7 cut(s) 76, 103, 347, 685, 706, 850, 1228
Hpy166II GTNNAC 6 cut(s) 274, 319, 325, 331, 714, 899
Hpy188I TCNGA 9 cut(s) 301, 387, 409, 475, 775, 790, 958, 1192, 1258
Hpy188III TCNNGA 7 cut(s) 143, 225, 553, 732, 1141, 1182, 1245
Hpy8I GTNNAC 6 cut(s) 274, 319, 325, 331, 714, 899
Hpy99I CGWCG 6 cut(s) 122, 137, 158, 161, 164, 167
HpyAV CCTTC 3 cut(s) 459, 676, 1231
HpyCH4III ACNGT 4 cut(s) 335, 352, 673, 1090
HpyCH4IV ACGT 4 cut(s) 315, 321, 327, 852
HpyCH4V TGCA 6 cut(s) 274, 820, 1029, 1123, 1161, 1230
HpyF3I CTNAG 4 cut(s) 123, 789, 902, 927
HpySE526I ACGT 4 cut(s) 315, 321, 327, 852
Hsp92I GRCGYC 1 cut(s) 1241
Hsp92II CATG 3 cut(s) 146, 544, 629
HspAI GCGC 3 cut(s) 240, 799, 1146
KroI GCCGGC 1 cut(s) 922
KroNI GCCGGC 1 cut(s) 924
KspI CCGCGG 1 cut(s) 1074
Kzo9I GATC 4 cut(s) 382, 608, 1137, 1258
LguI GCTCTTC 2 cut(s) 926, 1037
LmnI GCTCC 3 cut(s) 64, 734, 1081
Lsp1109I GCAGC 9 cut(s) 243, 495, 769, 924, 931, 1104, 1107, 1135, 1138
MaeII ACGT 4 cut(s) 315, 321, 327, 852
MaeIII GTNAC 3 cut(s) 82, 1095, 1242
MalI GATC 4 cut(s) 384, 610, 1139, 1260
MbiI CCGCTC 2 cut(s) 59, 1050
MboI GATC 4 cut(s) 382, 608, 1137, 1258
MflI RGATCY 2 cut(s) 608, 1137
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 2 cut(s) 494, 568
MlyI GAGTC 2 cut(s) 148, 305
MmeI TCCRAC 1 cut(s) 597
MroNI GCCGGC 1 cut(s) 922
MroXI GAANNNNTTC 1 cut(s) 665
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 279
MspA1I CMGCKG 2 cut(s) 249, 1073
MspI CCGG 9 cut(s) 360, 532, 581, 894, 923, 962, 1007, 1082, 1210
MspR9I CCNGG 6 cut(s) 361, 533, 581, 938, 1083, 1210
MvaI CCWGG 1 cut(s) 938
MvnI CGCG 2 cut(s) 858, 1073
NaeI GCCGGC 1 cut(s) 924
NciI CCSGG 5 cut(s) 361, 533, 581, 1083, 1210
NdeII GATC 4 cut(s) 382, 608, 1137, 1258
NgoMIV GCCGGC 1 cut(s) 922
NlaIII CATG 3 cut(s) 146, 544, 629
NlaIV GGNNCC 7 cut(s) 379, 438, 597, 610, 960, 1077, 1139
NmeAIII GCCGAG 1 cut(s) 1031
NmuCI GTSAC 3 cut(s) 82, 1095, 1242
NspV TTCGAA 2 cut(s) 806, 863
PagI TCATGA 1 cut(s) 142
PciSI GCTCTTC 2 cut(s) 926, 1037
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PdiI GCCGGC 1 cut(s) 924
PdmI GAANNNNTTC 1 cut(s) 665
PfeI GAWTC 6 cut(s) 388, 735, 1112, 1187, 1193, 1223
PleI GAGTC 2 cut(s) 147, 304
PpsI GAGTC 2 cut(s) 147, 304
PshAI GACNNNNGTC 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 936
PspGI CCWGG 1 cut(s) 936
PspN4I GGNNCC 7 cut(s) 379, 438, 597, 610, 960, 1077, 1139
PspPI GGNCC 3 cut(s) 71, 167, 578
PstI CTGCAG 1 cut(s) 1125
PsuI RGATCY 2 cut(s) 608, 1137
RsaI GTAC 3 cut(s) 615, 827, 985
RsaNI GTAC 3 cut(s) 614, 826, 984
RseI CAYNNNNRTG 1 cut(s) 279
SacII CCGCGG 1 cut(s) 1074
SapI GCTCTTC 2 cut(s) 926, 1037
SaqAI TTAA 1 cut(s) 342
Sau3AI GATC 4 cut(s) 382, 608, 1137, 1258
Sau96I GGNCC 3 cut(s) 71, 167, 578
ScaI AGTACT 2 cut(s) 827, 985
SchI GAGTC 2 cut(s) 148, 305
ScrFI CCNGG 6 cut(s) 361, 533, 581, 938, 1083, 1210
SduI GDGCHC 1 cut(s) 276
SfcI CTRYAG 2 cut(s) 1065, 1121
Sfr303I CCGCGG 1 cut(s) 1074
SfuI TTCGAA 2 cut(s) 806, 863
SgrBI CCGCGG 1 cut(s) 1074
SinI GGWCC 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 279
SmlI CTYRAG 3 cut(s) 20, 512, 551
SmoI CTYRAG 3 cut(s) 20, 512, 551
Sse9I AATT 2 cut(s) 494, 568
SspI AATATT 1 cut(s) 1003
StyD4I CCNGG 6 cut(s) 359, 531, 579, 936, 1081, 1208
StyI CCWWGG 1 cut(s) 486
TaaI ACNGT 4 cut(s) 335, 352, 673, 1090
TaiI ACGT 4 cut(s) 318, 324, 330, 855
TaqI TCGA 4 cut(s) 365, 395, 806, 863
TasI AATT 2 cut(s) 494, 568
TatI WGTACW 2 cut(s) 825, 983
TauI GCSGC 9 cut(s) 42, 95, 98, 101, 252, 968, 1050, 1073, 1076
TfiI GAWTC 6 cut(s) 388, 735, 1112, 1187, 1193, 1223
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 1 cut(s) 355
TseFI GTSAC 3 cut(s) 82, 1095, 1242
TseI GCWGC 9 cut(s) 231, 508, 782, 912, 919, 1117, 1120, 1123, 1126
Tsp45I GTSAC 3 cut(s) 82, 1095, 1242
TspDTI ATGAA 3 cut(s) 159, 529, 642
TspGWI ACGGA 4 cut(s) 180, 601, 608, 621
TspRI CASTG 1 cut(s) 355
VneI GTGCAC 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 665
ZrmI AGTACT 2 cut(s) 827, 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.