MD14G1115100.v1.1

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015380
Physical Location & Seq
Reverse (-)
18661470 .. 18663465
1996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1115100.v1.1.491

Sequence Viewer

Length: 1377 bp
ATGGAAACTTTCAGCTTCCTCAAGTACTGGCGCAGCGGTGGCCTACTTGCTGCCACCCCCGTTCCTAACAGCATAGACACGGTTGGTTCTGCTAACATGCACACCACCACCACCACCACCACCACAATCCTCACCGCCGTTGCCCAAAACGACGACGAAGACAGTGACAGCGACAACGACGACCGGCCTTTCTTCGACTTAGAGTTCGCCGTCCCCGACGAAGACGAAGCGGCTGCTCAAAACCACGACGAGGAAGCTCGGCAAGAGGATGACTGCAACGAGGATGTCCGGCAAGAAGACGACAGCGGCGGCGGCGACGACGACGACGACGAGGAGTCTGATGACAGTGAGGATGGAGAGGGGGAGTTTAACTTCACCGTCCCTTCCGGGTCGACCCAGGACAGAACGGTCGACCCGAATCTGACTCTCTCTCCCTCCGACGACTTGTTCTTCAAAGGGAAGCTTGTGCCTATCGAGCACTCGTCGATTGAGTTCAACCCATCTGAAGAATCTAACTCCAAGCCCCAATTCGCCGTCTCATTATTGAAATCGGCCACCAAATTTCGTGTTTTCATGCTGGGTTTGAAGAAATCGAAGTCGAATTCAAACGGGTCGGAAAAACCAGGGAAAACGGAGCTGCCGGCGGCTGGATCTGTTGCGGCGGTGGCGAAAGAACCACCGCAAAAACCCCAGGAAAAACCACCGCAAGAGGAACCGTCGCAAAAGCAGAAGCAGCAGGGGAAGCTTTTCACGGTGAAATTCAAGGTTCAGGAGGTTCCCATTGTGTCTTTGTTTGCCAGAGACAACAACTCCAGAATCTCTGCAACTAATAAGTCGCATACGACCCAGCGGAAGCCGCCGCCGATAACCGCTTCTGAAGAATCGGCTTCTGAGGAAAAGCGCTTTCCCAAAGAGGTAATGCAAAAGTACTTGAAAATGGTGAAGCCTCTCTACGTTCGCGTTTCAAAGAGGTATGGTGAGAAGCTGAGATTGTCCGGGCAGCTCAACTTGAACGGCTCGACGGCGCTGGCTTCGAATCAGGCCGAGAAGCCCCAGTCGGAAAGTGAAGTTTCAGAGCCGCCGCCGGCGACGGCGAGCAATGCGAAGAGTGGTCAGAAGCAGGGGAACTTTCCGGCAGGGCTGAGAGTGGTCTGCAAGCAATTGGGGAAGAGCCGGTCCGCTTCCTCCGCCGTAGCCGCGGCCCCATCGGCCGCCTTAGTCGCGCAGAGGAGAGATGATTCGCTGCTTCAGCAGCAGGACGGAATCCAAAGTGCCATCCTGCATTGCAAGCGTTCCTTCAATGCCTCCCGTGATTCGGACACCAACCGGATATCTCGGTCAGTGAGCGATTCCTCGAACGAAAAAAATGTCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

459

Amino Acids

49.88

Weight (kDa)

4.97

Isoelectric Point (pI)

57.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64080
fragaria_vesca FvH4_5g03470
malus_domestica MD06G1094800.v1.1 MD14G1115100.v1.1
prunus_persica Prupe.5G110000_v2.0.a1
pyrus_communis pycom06g09150
rosa_chinensis RchiOBHm_Chr7g0196191
rosa_laevigata RLG00000004053
rosa_multiflora Rmu_sc0007292.1_g000008
rosa_roxburghii Rroxscaffold_3G00259680
rosa_rugosa Rorug07G0032000
rosa_samantha Rh7AG157400 Rh7BG159100 Rh7CG163100 Rh7DG158400
rosa_wichuraiana Rw7G013460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 407
AccI GTMKAC 2 cut(s) 392, 411
AccII CGCG 3 cut(s) 960, 1199, 1223
AclWI GGATC 1 cut(s) 658
AcoI YGGCCR 2 cut(s) 552, 1209
AcsI RAATTY 3 cut(s) 560, 601, 758
AcuI CTGAAG 3 cut(s) 525, 897, 1232
AfaI GTAC 2 cut(s) 26, 929
AfeI AGCGCT 1 cut(s) 902
AfiI CCNNNNNNNGG 3 cut(s) 250, 647, 1315
AhdI GACNNNNNGTC 1 cut(s) 334
AjnI CCWGG 3 cut(s) 396, 622, 690
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AluBI AGCT 7 cut(s) 15, 257, 463, 637, 745, 985, 1003
AluI AGCT 7 cut(s) 15, 257, 463, 637, 745, 985, 1003
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 2 cut(s) 541, 795
AlwI GGATC 1 cut(s) 658
Aor51HI AGCGCT 1 cut(s) 902
AoxI GGCC 6 cut(s) 40, 185, 552, 1041, 1200, 1209
ApeKI GCWGC 8 cut(s) 33, 50, 233, 637, 733, 1000, 1243, 1252
ApoI RAATTY 3 cut(s) 560, 601, 758
ArsI GACNNNNNNTTYG 3 cut(s) 188, 220, 1353
Asp700I GAANNNNTTC 1 cut(s) 746
AspLEI GCGC 4 cut(s) 33, 903, 1027, 1225
AspS9I GGNCC 2 cut(s) 1176, 1201
AsuC2I CCSGG 2 cut(s) 388, 997
AsuHPI GGTGA 5 cut(s) 124, 367, 766, 952, 989
AsuII TTCGAA 1 cut(s) 1034
AvaII GGWCC 1 cut(s) 1176
BarI GAAGNNNNNNTAC 2 cut(s) 935, 967
BbsI GAAGAC 3 cut(s) 165, 228, 303
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 8 cut(s) 37, 45, 220, 624, 745, 1012, 1230, 1264
BccI CCATC 4 cut(s) 347, 508, 1213, 1283
BceAI ACGGC 7 cut(s) 122, 194, 518, 1030, 1038, 1107, 1175
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 3 cut(s) 398, 624, 692
BcnI CCSGG 2 cut(s) 388, 997
BcoDI GTCTC 2 cut(s) 541, 795
BfoI RGCGCY 2 cut(s) 904, 1028
BglI GCCNNNNNGGC 1 cut(s) 1208
BmcAI AGTACT 2 cut(s) 26, 929
Bme1390I CCNGG 5 cut(s) 388, 398, 624, 692, 997
Bme18I GGWCC 1 cut(s) 1176
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 1176, 1201
BmiI GGNNCC 3 cut(s) 714, 777, 1203
BmrFI CCNGG 5 cut(s) 388, 398, 624, 692, 997
BmrI ACTGGG 1 cut(s) 1048
BmuI ACTGGG 1 cut(s) 1048
BpiI GAAGAC 3 cut(s) 165, 228, 303
BpmI CTGGAG 1 cut(s) 796
Bpu14I TTCGAA 1 cut(s) 1034
BpuEI CTTGAG 1 cut(s) 5
BpuMI CCSGG 2 cut(s) 388, 997
BsaJI CCNNGG 4 cut(s) 396, 623, 690, 1197
BsaWI WCCGGW 1 cut(s) 1326
BsaXI ACNNNNNCTCC 4 cut(s) 415, 445, 794, 824
Bsc4I CCNNNNNNNGG 3 cut(s) 250, 647, 1315
Bse118I RCCGGY 4 cut(s) 183, 640, 1084, 1173
Bse1I ACTGG 2 cut(s) 32, 1054
Bse3DI GCAATG 2 cut(s) 1105, 1282
BseBI CCWGG 3 cut(s) 398, 624, 692
BseDI CCNNGG 4 cut(s) 396, 623, 690, 1197
BseGI GGATG 4 cut(s) 274, 289, 358, 1275
BseLI CCNNNNNNNGG 3 cut(s) 250, 647, 1315
BseMI GCAATG 2 cut(s) 1105, 1282
BseMII CTCAG 3 cut(s) 882, 977, 1133
BseNI ACTGG 2 cut(s) 32, 1054
BseRI GAGGAG 2 cut(s) 347, 1243
BseX3I CGGCCG 1 cut(s) 1209
BseXI GCAGC 8 cut(s) 37, 45, 220, 624, 745, 1012, 1230, 1264
BseYI CCCAGC 2 cut(s) 577, 846
Bsh1236I CGCG 3 cut(s) 960, 1199, 1223
Bsh1285I CGRYCG 3 cut(s) 184, 411, 1212
BshFI GGCC 6 cut(s) 42, 187, 554, 1043, 1202, 1211
BsiEI CGRYCG 3 cut(s) 184, 411, 1212
BsiHKAI GWGCWC 1 cut(s) 480
BsiSI CCGG 9 cut(s) 184, 289, 387, 641, 996, 1085, 1133, 1174, 1327
BslFI GGGAC 2 cut(s) 197, 365
BslI CCNNNNNNNGG 3 cut(s) 250, 647, 1315
BsmAI GTCTC 2 cut(s) 541, 795
BsmBI CGTCTC 1 cut(s) 541
BsmFI GGGAC 2 cut(s) 197, 365
BsnI GGCC 6 cut(s) 42, 187, 554, 1043, 1202, 1211
Bsp119I TTCGAA 1 cut(s) 1034
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 1 cut(s) 650
BspANI GGCC 6 cut(s) 42, 187, 554, 1043, 1202, 1211
BspCNI CTCAG 3 cut(s) 883, 978, 1134
BspFNI CGCG 3 cut(s) 960, 1199, 1223
BspLI GGNNCC 3 cut(s) 714, 777, 1203
BspPI GGATC 1 cut(s) 658
BspQI GCTCTTC 1 cut(s) 1163
BspT104I TTCGAA 1 cut(s) 1034
BsrDI GCAATG 2 cut(s) 1105, 1282
BsrFI RCCGGY 4 cut(s) 183, 640, 1084, 1173
BsrI ACTGG 2 cut(s) 32, 1054
BssAI RCCGGY 4 cut(s) 183, 640, 1084, 1173
BssECI CCNNGG 4 cut(s) 396, 623, 690, 1197
BssMI GATC 1 cut(s) 650
Bst2UI CCWGG 3 cut(s) 398, 624, 692
Bst4CI ACNGT 7 cut(s) 82, 164, 347, 379, 409, 717, 754
Bst6I CTCTTC 2 cut(s) 1100, 1163
BstBI TTCGAA 1 cut(s) 1034
BstC8I GCNNGC 6 cut(s) 642, 1029, 1086, 1096, 1157, 1289
BstDEI CTNAG 5 cut(s) 199, 891, 986, 1142, 1216
BstDSI CCRYGG 1 cut(s) 1197
BstF5I GGATG 4 cut(s) 274, 289, 358, 1275
BstFNI CGCG 3 cut(s) 960, 1199, 1223
BstH2I RGCGCY 2 cut(s) 904, 1028
BstHHI GCGC 4 cut(s) 33, 903, 1027, 1225
BstKTI GATC 1 cut(s) 653
BstMAI GTCTC 2 cut(s) 541, 795
BstMBI GATC 1 cut(s) 650
BstMCI CGRYCG 3 cut(s) 184, 411, 1212
BstNI CCWGG 3 cut(s) 398, 624, 692
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 5 cut(s) 386, 396, 622, 690, 995
BstUI CGCG 3 cut(s) 960, 1199, 1223
BstV1I GCAGC 8 cut(s) 37, 45, 220, 624, 745, 1012, 1230, 1264
BstV2I GAAGAC 3 cut(s) 165, 228, 303
BstX2I RGATCY 1 cut(s) 650
BstYI RGATCY 1 cut(s) 650
BstZI CGGCCG 1 cut(s) 1209
BsuRI GGCC 6 cut(s) 42, 187, 554, 1043, 1202, 1211
BtgI CCRYGG 1 cut(s) 1197
BtsCI GGATG 4 cut(s) 274, 289, 358, 1275
BtsIMutI CAGTG 3 cut(s) 169, 352, 1347
Cac8I GCNNGC 6 cut(s) 642, 1029, 1086, 1096, 1157, 1289
CfoI GCGC 4 cut(s) 33, 903, 1027, 1225
Cfr10I RCCGGY 4 cut(s) 183, 640, 1084, 1173
Cfr13I GGNCC 2 cut(s) 1176, 1201
Cfr42I CCGCGG 1 cut(s) 1200
CpoI CGGWCCG 1 cut(s) 1176
Csp6I GTAC 2 cut(s) 25, 928
CspI CGGWCCG 1 cut(s) 1176
CviAII CATG 2 cut(s) 97, 574
CviQI GTAC 2 cut(s) 25, 928
DdeI CTNAG 5 cut(s) 199, 891, 986, 1142, 1216
DpnI GATC 1 cut(s) 652
DpnII GATC 1 cut(s) 650
DrdI GACNNNNNNGTC 1 cut(s) 407
DriI GACNNNNNGTC 1 cut(s) 334
DseDI GACNNNNNNGTC 1 cut(s) 407
EaeI YGGCCR 2 cut(s) 552, 1209
EagI CGGCCG 1 cut(s) 1209
Eam1104I CTCTTC 2 cut(s) 1100, 1163
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 2 cut(s) 1100, 1163
EciI GGCGGA 1 cut(s) 1177
EclXI CGGCCG 1 cut(s) 1209
Eco32I GATATC 1 cut(s) 1332
Eco47I GGWCC 1 cut(s) 1176
Eco47III AGCGCT 1 cut(s) 902
Eco52I CGGCCG 1 cut(s) 1209
Eco57I CTGAAG 3 cut(s) 525, 897, 1232
EcoRI GAATTC 1 cut(s) 601
EcoRII CCWGG 3 cut(s) 396, 622, 690
EcoRV GATATC 1 cut(s) 1332
Esp3I CGTCTC 1 cut(s) 541
FaeI CATG 2 cut(s) 100, 577
FaiI YATR 5 cut(s) 74, 98, 575, 840, 975
FalI AAGNNNNNCTT 4 cut(s) 447, 479, 1280, 1312
FaqI GGGAC 2 cut(s) 197, 365
FatI CATG 2 cut(s) 96, 573
FblI GTMKAC 2 cut(s) 392, 411
FokI GGATG 4 cut(s) 281, 296, 365, 1262
GlaI GCGC 4 cut(s) 32, 902, 1026, 1224
GsaI CCCAGC 2 cut(s) 581, 850
GsuI CTGGAG 1 cut(s) 796
HaeII RGCGCY 2 cut(s) 904, 1028
HaeIII GGCC 6 cut(s) 42, 187, 554, 1043, 1202, 1211
HapII CCGG 9 cut(s) 184, 289, 387, 641, 996, 1085, 1133, 1174, 1327
HhaI GCGC 4 cut(s) 33, 903, 1027, 1225
Hin1II CATG 2 cut(s) 100, 577
Hin6I GCGC 4 cut(s) 31, 901, 1025, 1223
HinP1I GCGC 4 cut(s) 31, 901, 1025, 1223
HincII GTYRAC 2 cut(s) 393, 412
HindII GTYRAC 2 cut(s) 393, 412
HindIII AAGCTT 2 cut(s) 461, 743
HpaII CCGG 9 cut(s) 184, 289, 387, 641, 996, 1085, 1133, 1174, 1327
HphI GGTGA 5 cut(s) 124, 367, 766, 952, 989
Hpy166II GTNNAC 2 cut(s) 393, 412
Hpy188III TCNNGA 2 cut(s) 770, 813
Hpy8I GTNNAC 2 cut(s) 393, 412
HpyAV CCTTC 2 cut(s) 393, 1306
HpyCH4III ACNGT 7 cut(s) 82, 164, 347, 379, 409, 717, 754
HpyCH4IV ACGT 1 cut(s) 954
HpyCH4V TGCA 7 cut(s) 100, 276, 824, 922, 1155, 1282, 1287
HpyF3I CTNAG 5 cut(s) 199, 891, 986, 1142, 1216
HpySE526I ACGT 1 cut(s) 954
Hsp92II CATG 2 cut(s) 100, 577
HspAI GCGC 4 cut(s) 31, 901, 1025, 1223
KroI GCCGGC 2 cut(s) 640, 1084
KroNI GCCGGC 2 cut(s) 642, 1086
KspI CCGCGG 1 cut(s) 1200
Kzo9I GATC 1 cut(s) 650
LguI GCTCTTC 1 cut(s) 1163
LmnI GCTCC 1 cut(s) 634
Lsp1109I GCAGC 8 cut(s) 37, 45, 220, 624, 745, 1012, 1230, 1264
MaeII ACGT 1 cut(s) 954
MaeIII GTNAC 1 cut(s) 164
MalI GATC 1 cut(s) 652
MboI GATC 1 cut(s) 650
MfeI CAATTG 1 cut(s) 1160
MflI RGATCY 1 cut(s) 650
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 5 cut(s) 527, 560, 601, 758, 1160
MlyI GAGTC 2 cut(s) 344, 418
MmeI TCCRAC 3 cut(s) 462, 594, 1038
MreI CGCCGGCG 1 cut(s) 1084
MroNI GCCGGC 2 cut(s) 640, 1084
MroXI GAANNNNTTC 1 cut(s) 746
MseI TTAA 1 cut(s) 369
MspA1I CMGCKG 4 cut(s) 36, 306, 850, 1199
MspI CCGG 9 cut(s) 184, 289, 387, 641, 996, 1085, 1133, 1174, 1327
MspR9I CCNGG 5 cut(s) 388, 398, 624, 692, 997
MunI CAATTG 1 cut(s) 1160
MvaI CCWGG 3 cut(s) 398, 624, 692
MvnI CGCG 3 cut(s) 960, 1199, 1223
NaeI GCCGGC 2 cut(s) 642, 1086
NciI CCSGG 2 cut(s) 388, 997
NdeII GATC 1 cut(s) 650
NgoMIV GCCGGC 2 cut(s) 640, 1084
NlaIII CATG 2 cut(s) 100, 577
NlaIV GGNNCC 3 cut(s) 714, 777, 1203
NmeAIII GCCGAG 2 cut(s) 238, 1069
NmuCI GTSAC 1 cut(s) 164
NspI RCATGY 1 cut(s) 100
NspV TTCGAA 1 cut(s) 1034
PciSI GCTCTTC 1 cut(s) 1163
PcsI WCGNNNNNNNCGW 4 cut(s) 213, 324, 327, 413
PdiI GCCGGC 2 cut(s) 642, 1086
PdmI GAANNNNTTC 1 cut(s) 746
PfeI GAWTC 9 cut(s) 418, 509, 816, 881, 1036, 1238, 1263, 1313, 1349
PleI GAGTC 2 cut(s) 343, 418
PpsI GAGTC 2 cut(s) 343, 418
Psp6I CCWGG 3 cut(s) 396, 622, 690
PspFI CCCAGC 2 cut(s) 577, 846
PspGI CCWGG 3 cut(s) 396, 622, 690
PspN4I GGNNCC 3 cut(s) 714, 777, 1203
PspPI GGNCC 2 cut(s) 1176, 1201
PsuI RGATCY 1 cut(s) 650
RsaI GTAC 2 cut(s) 26, 929
RsaNI GTAC 2 cut(s) 25, 928
Rsr2I CGGWCCG 1 cut(s) 1176
RsrII CGGWCCG 1 cut(s) 1176
SacII CCGCGG 1 cut(s) 1200
SalI GTCGAC 2 cut(s) 391, 410
SapI GCTCTTC 1 cut(s) 1163
SaqAI TTAA 1 cut(s) 369
Sau3AI GATC 1 cut(s) 650
Sau96I GGNCC 2 cut(s) 1176, 1201
ScaI AGTACT 2 cut(s) 26, 929
SchI GAGTC 2 cut(s) 344, 418
ScrFI CCNGG 5 cut(s) 388, 398, 624, 692, 997
SduI GDGCHC 1 cut(s) 480
SfiI GGCCNNNNNGGCC 1 cut(s) 1208
Sfr303I CCGCGG 1 cut(s) 1200
SfuI TTCGAA 1 cut(s) 1034
SgrAI CRCCGGYG 1 cut(s) 1084
SgrBI CCGCGG 1 cut(s) 1200
SinI GGWCC 1 cut(s) 1176
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 5 cut(s) 527, 560, 601, 758, 1160
StyD4I CCNGG 5 cut(s) 386, 396, 622, 690, 995
TaaI ACNGT 7 cut(s) 82, 164, 347, 379, 409, 717, 754
TaiI ACGT 1 cut(s) 957
TaqII GACCGA 1 cut(s) 1326
TasI AATT 5 cut(s) 527, 560, 601, 758, 1160
TatI WGTACW 2 cut(s) 24, 927
TfiI GAWTC 9 cut(s) 418, 509, 816, 881, 1036, 1238, 1263, 1313, 1349
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 3 cut(s) 169, 352, 1347
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 8 cut(s) 33, 50, 233, 637, 733, 1000, 1243, 1252
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 1 cut(s) 562
TspGWI ACGGA 2 cut(s) 647, 1275
TspRI CASTG 3 cut(s) 169, 352, 1347
VpaK11BI GGWCC 1 cut(s) 1176
XapI RAATTY 3 cut(s) 560, 601, 758
XceI RCATGY 1 cut(s) 100
XmiI GTMKAC 2 cut(s) 392, 411
XmnI GAANNNNTTC 1 cut(s) 746
ZrmI AGTACT 2 cut(s) 26, 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.