Rw7G013460

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0015380
Physical Location & Seq
Forward (+)
12963968 .. 12965404
1437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G013460.1

Sequence Viewer

Length: 1248 bp
ATGGAAGCTTTTAGCCTGCTCAAGTATTGGCGAGGTGGCGGCCTTGTTGCTTCCTCCGCTCCTCCTAATGGCCCTAACAATGTCACCTCCGCCGCCGCCGCCTCCACCATCCTCACCGCCGTCGCTGAGAACAGCGACGAGAGTCATGAAACTGACGACGACGACGACGACGGACCTTTCTTTGACTTGGAGTTTGCCGTCCCCGACGAGGCCGAAGCCCACGAAGCCTCTGGAAGCAGCGACAGCGCCACCAGCGGCGAAGAGAGTGAGGTGGAAGTGCACCAAGATGGGGAAGATGATGAGTCCGATGATGATGATGATGACGACGATGACGTGGACGTGGACGGTGAGGAGGGTGAGTTTAACTTCACTGTGTCTTCCGGGTCGAGCAATGACGGCACCGATCCGAATCTGTCGATGTCTCCCTCTGACGATTTGTTTTCAAGGCCGAACTCCAAGGCTCAATTCGCAGGGTCGCTGCTCAAGTCCGCCACCAAGTTCCGGGTCTTCATGTCGGGTCTCAAGAAATCCAAGTCCAATTCCAACGGCCCGGAAAACAAAACGGAACCTACAAACGGATCCGTACAAGAACAACATGAAAAACAAGAGAATAAACAACAGAAGCAGGGGAAGCTTTTCACGGTGAAGTTGAAGGTGGAGGAAGTTCCCATTGTGTCCTTGTTCACCAGAGACAACAGCTCCAGAATCTCCACCAAGACCCAGAACCAGAAAACCCAGAGTTCCGAAGAAGCTGCTTCTGAGGAGAAGCGCTTTTCGAAAGAGGTAATGCAGAAGTACTTGAAAATGGTGAAGCCTCTCTACGTCCGAGTTTCGAAACGCTACGGCGAGAAGACGAGGCTCTCCGGTCAACTCAGCGTAAGCAGCCCGCAGCCGGCGGAGAAGAGCCAGGTGGAGGCTGAAGTTTCGGAACCGGCGGCGAGCAATGTGAAGACTACTTTGAAGCAGGGGAATATTCCGGCGGGTTTGAGAGTTGTTTGCAAGCACTTGGGGAAGAGCCGCTCGGCTTCTTCGGCTGTAGCCGCGGCTCCACCGGGAACTGTGCTGTCACAGAGGAGAGATGATTCGCTGCTGCAGCAGCAGGATGGGATCCAGAGCGCCATTCTCCATTGCAAGCGGTCATTCAATGCTTCCAGAGATTCCGATTCTACTCCGCTATCCCGGTCGGTGAGCGATTCCTGCAACGAGAAGGCGTCACGAAAACCGTCGGATCAAGGCGGAGAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

415

Amino Acids

44.7

Weight (kDa)

4.74

Isoelectric Point (pI)

52.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64080
fragaria_vesca FvH4_5g03470
malus_domestica MD06G1094800.v1.1 MD14G1115100.v1.1
prunus_persica Prupe.5G110000_v2.0.a1
pyrus_communis pycom06g09150
rosa_chinensis RchiOBHm_Chr7g0196191
rosa_laevigata RLG00000004053
rosa_multiflora Rmu_sc0007292.1_g000008
rosa_roxburghii Rroxscaffold_3G00259680
rosa_rugosa Rorug07G0032000
rosa_samantha Rh7AG157400 Rh7BG159100 Rh7CG163100 Rh7DG158400
rosa_wichuraiana Rw7G013460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 398
AccBSI CCGCTC 2 cut(s) 59, 1020
AccII CGCG 1 cut(s) 1043
AclWI GGATC 6 cut(s) 398, 573, 586, 1102, 1115, 1236
AcuI CTGAAG 1 cut(s) 939
AcyI GRCGYC 1 cut(s) 1211
AfaI GTAC 2 cut(s) 585, 797
AfeI AGCGCT 1 cut(s) 770
AfiI CCNNNNNNNGG 7 cut(s) 68, 208, 289, 501, 575, 892, 913
AgsI TTSAA 5 cut(s) 444, 652, 802, 961, 1144
AjiI CACGTC 2 cut(s) 334, 340
AjnI CCWGG 1 cut(s) 906
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
AluBI AGCT 4 cut(s) 8, 634, 699, 752
AluI AGCT 4 cut(s) 8, 634, 699, 752
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 3 cut(s) 426, 524, 684
Alw44I GTGCAC 1 cut(s) 278
AlwI GGATC 6 cut(s) 398, 573, 586, 1102, 1115, 1236
Aor51HI AGCGCT 1 cut(s) 770
AoxI GGCC 5 cut(s) 40, 70, 210, 446, 547
ApaLI GTGCAC 1 cut(s) 278
ApeKI GCWGC 9 cut(s) 237, 478, 752, 882, 889, 1087, 1090, 1093, 1096
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
Asp700I GAANNNNTTC 1 cut(s) 635
AspLEI GCGC 3 cut(s) 248, 771, 1118
AspS9I GGNCC 3 cut(s) 71, 173, 548
AsuC2I CCSGG 5 cut(s) 382, 503, 551, 1053, 1180
AsuHPI GGTGA 8 cut(s) 76, 106, 359, 368, 655, 676, 820, 1198
AsuII TTCGAA 2 cut(s) 776, 833
AvaII GGWCC 1 cut(s) 173
BaeGI GKGCMC 1 cut(s) 282
BamHI GGATCC 2 cut(s) 578, 1107
BanI GGYRCC 1 cut(s) 398
BarI GAAGNNNNNNTAC 2 cut(s) 803, 835
BbsI GAAGAC 4 cut(s) 369, 499, 857, 956
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 9 cut(s) 249, 465, 739, 894, 901, 1074, 1077, 1105, 1108
BccI CCATC 3 cut(s) 116, 281, 1097
BceAI ACGGC 5 cut(s) 104, 182, 412, 562, 859
BcgI CGANNNNNNTGC 2 cut(s) 734, 768
BciT130I CCWGG 1 cut(s) 908
BcnI CCSGG 5 cut(s) 382, 503, 551, 1053, 1180
BcoDI GTCTC 3 cut(s) 426, 524, 684
BfmI CTRYAG 2 cut(s) 1035, 1091
BfoI RGCGCY 3 cut(s) 249, 772, 1119
BmcAI AGTACT 1 cut(s) 797
Bme1390I CCNGG 6 cut(s) 382, 503, 551, 908, 1053, 1180
Bme18I GGWCC 1 cut(s) 173
BmgBI CACGTC 2 cut(s) 334, 340
BmgT120I GGNCC 3 cut(s) 71, 173, 548
BmiI GGNNCC 6 cut(s) 400, 567, 580, 930, 1047, 1109
BmrFI CCNGG 6 cut(s) 382, 503, 551, 908, 1053, 1180
BoxI GACNNNNGTC 1 cut(s) 141
BpiI GAAGAC 4 cut(s) 369, 499, 857, 956
BpmI CTGGAG 1 cut(s) 685
Bpu14I TTCGAA 2 cut(s) 776, 833
BpuEI CTTGAG 3 cut(s) 5, 467, 506
BpuMI CCSGG 5 cut(s) 382, 503, 551, 1053, 1180
BsaBI GATNNNNATC 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 1211
BsaI GGTCTC 1 cut(s) 524
BsaJI CCNNGG 2 cut(s) 456, 1041
BsaWI WCCGGW 1 cut(s) 863
BsaXI ACNNNNNCTCC 4 cut(s) 344, 374, 683, 713
Bsc4I CCNNNNNNNGG 7 cut(s) 68, 208, 289, 501, 575, 892, 913
Bse118I RCCGGY 2 cut(s) 892, 931
Bse3DI GCAATG 3 cut(s) 397, 949, 1126
Bse8I GATNNNNATC 1 cut(s) 408
BseBI CCWGG 1 cut(s) 908
BseDI CCNNGG 2 cut(s) 456, 1041
BseGI GGATG 2 cut(s) 108, 1108
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 7 cut(s) 68, 208, 289, 501, 575, 892, 913
BseMI GCAATG 3 cut(s) 397, 949, 1126
BseMII CTCAG 3 cut(s) 117, 750, 886
BseRI GAGGAG 4 cut(s) 51, 365, 776, 1087
BseSI GKGCMC 1 cut(s) 282
BseXI GCAGC 9 cut(s) 249, 465, 739, 894, 901, 1074, 1077, 1105, 1108
Bsh1236I CGCG 1 cut(s) 1043
Bsh1285I CGRYCG 1 cut(s) 1184
BshFI GGCC 5 cut(s) 42, 72, 212, 448, 549
BshNI GGYRCC 1 cut(s) 398
BsiEI CGRYCG 1 cut(s) 1184
BsiHKAI GWGCWC 1 cut(s) 282
BsiSI CCGG 9 cut(s) 381, 502, 551, 864, 893, 932, 977, 1052, 1180
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 7 cut(s) 68, 208, 289, 501, 575, 892, 913
BsmAI GTCTC 3 cut(s) 426, 524, 684
BsmFI GGGAC 1 cut(s) 185
BsnI GGCC 5 cut(s) 42, 72, 212, 448, 549
Bso31I GGTCTC 1 cut(s) 524
Bsp119I TTCGAA 2 cut(s) 776, 833
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 4 cut(s) 403, 578, 1107, 1228
BspANI GGCC 5 cut(s) 42, 72, 212, 448, 549
BspCNI CTCAG 3 cut(s) 118, 751, 885
BspFNI CGCG 1 cut(s) 1043
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 6 cut(s) 400, 567, 580, 930, 1047, 1109
BspMAI CTGCAG 1 cut(s) 1095
BspPI GGATC 6 cut(s) 398, 573, 586, 1102, 1115, 1236
BspQI GCTCTTC 2 cut(s) 896, 1007
BspT104I TTCGAA 2 cut(s) 776, 833
BspT107I GGYRCC 1 cut(s) 398
BspTNI GGTCTC 1 cut(s) 524
BsrBI CCGCTC 2 cut(s) 59, 1020
BsrDI GCAATG 3 cut(s) 397, 949, 1126
BsrFI RCCGGY 2 cut(s) 892, 931
BssAI RCCGGY 2 cut(s) 892, 931
BssECI CCNNGG 2 cut(s) 456, 1041
BssMI GATC 4 cut(s) 403, 578, 1107, 1228
BssNI GRCGYC 1 cut(s) 1211
BssT1I CCWWGG 1 cut(s) 456
Bst2UI CCWGG 1 cut(s) 908
Bst4CI ACNGT 5 cut(s) 347, 373, 643, 1060, 1224
Bst6I CTCTTC 3 cut(s) 255, 896, 1007
BstACI GRCGYC 1 cut(s) 1211
BstBI TTCGAA 2 cut(s) 776, 833
BstC8I GCNNGC 6 cut(s) 17, 887, 894, 940, 1001, 1133
BstDEI CTNAG 3 cut(s) 126, 759, 872
BstDSI CCRYGG 1 cut(s) 1041
BstF5I GGATG 2 cut(s) 108, 1108
BstFNI CGCG 1 cut(s) 1043
BstH2I RGCGCY 3 cut(s) 249, 772, 1119
BstHHI GCGC 3 cut(s) 248, 771, 1118
BstKTI GATC 4 cut(s) 406, 581, 1110, 1231
BstMAI GTCTC 3 cut(s) 426, 524, 684
BstMBI GATC 4 cut(s) 403, 578, 1107, 1228
BstMCI CGRYCG 1 cut(s) 1184
BstNI CCWGG 1 cut(s) 908
BstPAI GACNNNNGTC 1 cut(s) 141
BstSCI CCNGG 6 cut(s) 380, 501, 549, 906, 1051, 1178
BstSFI CTRYAG 2 cut(s) 1035, 1091
BstSLI GKGCMC 1 cut(s) 282
BstUI CGCG 1 cut(s) 1043
BstV1I GCAGC 9 cut(s) 249, 465, 739, 894, 901, 1074, 1077, 1105, 1108
BstV2I GAAGAC 4 cut(s) 369, 499, 857, 956
BstX2I RGATCY 2 cut(s) 578, 1107
BstYI RGATCY 2 cut(s) 578, 1107
BsuRI GGCC 5 cut(s) 42, 72, 212, 448, 549
BtgI CCRYGG 1 cut(s) 1041
BtrI CACGTC 2 cut(s) 334, 340
BtsCI GGATG 2 cut(s) 108, 1108
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 6 cut(s) 17, 887, 894, 940, 1001, 1133
CciI TCATGA 1 cut(s) 145
CfoI GCGC 3 cut(s) 248, 771, 1118
Cfr10I RCCGGY 2 cut(s) 892, 931
Cfr13I GGNCC 3 cut(s) 71, 173, 548
Cfr42I CCGCGG 1 cut(s) 1044
CseI GACGC 1 cut(s) 1200
Csp6I GTAC 2 cut(s) 584, 796
CviAII CATG 3 cut(s) 146, 511, 596
CviQI GTAC 2 cut(s) 584, 796
DdeI CTNAG 3 cut(s) 126, 759, 872
DpnI GATC 4 cut(s) 405, 580, 1109, 1230
DpnII GATC 4 cut(s) 403, 578, 1107, 1228
Eam1104I CTCTTC 3 cut(s) 255, 896, 1007
EarI CTCTTC 3 cut(s) 255, 896, 1007
EciI GGCGGA 3 cut(s) 79, 478, 911
Eco130I CCWWGG 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 524
Eco47I GGWCC 1 cut(s) 173
Eco47III AGCGCT 1 cut(s) 770
Eco57I CTGAAG 1 cut(s) 939
EcoRII CCWGG 1 cut(s) 906
EcoT14I CCWWGG 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 456
FaeI CATG 3 cut(s) 149, 514, 599
FaiI YATR 3 cut(s) 147, 512, 597
FaqI GGGAC 1 cut(s) 185
FatI CATG 3 cut(s) 145, 510, 595
FauI CCCGC 2 cut(s) 894, 973
FokI GGATG 2 cut(s) 95, 1115
GlaI GCGC 3 cut(s) 247, 770, 1117
GsuI CTGGAG 1 cut(s) 685
HaeII RGCGCY 3 cut(s) 249, 772, 1119
HaeIII GGCC 5 cut(s) 42, 72, 212, 448, 549
HapII CCGG 9 cut(s) 381, 502, 551, 864, 893, 932, 977, 1052, 1180
HgaI GACGC 1 cut(s) 1200
HhaI GCGC 3 cut(s) 248, 771, 1118
Hin1I GRCGYC 1 cut(s) 1211
Hin1II CATG 3 cut(s) 149, 514, 599
Hin6I GCGC 3 cut(s) 246, 769, 1116
HinP1I GCGC 3 cut(s) 246, 769, 1116
HincII GTYRAC 1 cut(s) 869
HindII GTYRAC 1 cut(s) 869
HindIII AAGCTT 2 cut(s) 6, 632
HinfI GANTC 8 cut(s) 142, 302, 409, 705, 1082, 1157, 1163, 1193
HpaII CCGG 9 cut(s) 381, 502, 551, 864, 893, 932, 977, 1052, 1180
HphI GGTGA 8 cut(s) 76, 106, 359, 368, 655, 676, 820, 1198
Hpy166II GTNNAC 5 cut(s) 280, 337, 343, 684, 869
Hpy188I TCNGA 9 cut(s) 307, 408, 430, 745, 760, 827, 928, 1162, 1228
Hpy188III TCNNGA 7 cut(s) 146, 231, 523, 702, 1111, 1152, 1215
Hpy8I GTNNAC 5 cut(s) 280, 337, 343, 684, 869
HpyAV CCTTC 2 cut(s) 646, 1201
HpyCH4III ACNGT 5 cut(s) 347, 373, 643, 1060, 1224
HpyCH4IV ACGT 3 cut(s) 333, 339, 822
HpyCH4V TGCA 6 cut(s) 280, 790, 999, 1093, 1131, 1200
HpyF3I CTNAG 3 cut(s) 126, 759, 872
HpySE526I ACGT 3 cut(s) 333, 339, 822
Hsp92I GRCGYC 1 cut(s) 1211
Hsp92II CATG 3 cut(s) 149, 514, 599
HspAI GCGC 3 cut(s) 246, 769, 1116
KroI GCCGGC 1 cut(s) 892
KroNI GCCGGC 1 cut(s) 894
KspI CCGCGG 1 cut(s) 1044
Kzo9I GATC 4 cut(s) 403, 578, 1107, 1228
LguI GCTCTTC 2 cut(s) 896, 1007
LmnI GCTCC 3 cut(s) 64, 704, 1051
Lsp1109I GCAGC 9 cut(s) 249, 465, 739, 894, 901, 1074, 1077, 1105, 1108
MaeII ACGT 3 cut(s) 333, 339, 822
MaeIII GTNAC 3 cut(s) 82, 1065, 1212
MalI GATC 4 cut(s) 405, 580, 1109, 1230
MbiI CCGCTC 2 cut(s) 59, 1020
MboI GATC 4 cut(s) 403, 578, 1107, 1228
MflI RGATCY 2 cut(s) 578, 1107
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 2 cut(s) 464, 538
MlyI GAGTC 2 cut(s) 151, 311
MmeI TCCRAC 2 cut(s) 567, 1206
MroNI GCCGGC 1 cut(s) 892
MroXI GAANNNNTTC 1 cut(s) 635
MseI TTAA 1 cut(s) 363
MslI CAYNNNNRTG 1 cut(s) 285
MspA1I CMGCKG 2 cut(s) 255, 1043
MspI CCGG 9 cut(s) 381, 502, 551, 864, 893, 932, 977, 1052, 1180
MspR9I CCNGG 6 cut(s) 382, 503, 551, 908, 1053, 1180
MvaI CCWGG 1 cut(s) 908
MvnI CGCG 1 cut(s) 1043
NaeI GCCGGC 1 cut(s) 894
NciI CCSGG 5 cut(s) 382, 503, 551, 1053, 1180
NdeII GATC 4 cut(s) 403, 578, 1107, 1228
NgoMIV GCCGGC 1 cut(s) 892
NlaIII CATG 3 cut(s) 149, 514, 599
NlaIV GGNNCC 6 cut(s) 400, 567, 580, 930, 1047, 1109
NmeAIII GCCGAG 1 cut(s) 1001
NmuCI GTSAC 3 cut(s) 82, 1065, 1212
NspV TTCGAA 2 cut(s) 776, 833
PagI TCATGA 1 cut(s) 145
PciSI GCTCTTC 2 cut(s) 896, 1007
PcsI WCGNNNNNNNCGW 3 cut(s) 162, 165, 330
PdiI GCCGGC 1 cut(s) 894
PdmI GAANNNNTTC 1 cut(s) 635
PfeI GAWTC 6 cut(s) 409, 705, 1082, 1157, 1163, 1193
PleI GAGTC 2 cut(s) 150, 310
PpsI GAGTC 2 cut(s) 150, 310
PshAI GACNNNNGTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 906
PspGI CCWGG 1 cut(s) 906
PspN4I GGNNCC 6 cut(s) 400, 567, 580, 930, 1047, 1109
PspPI GGNCC 3 cut(s) 71, 173, 548
PstI CTGCAG 1 cut(s) 1095
PsuI RGATCY 2 cut(s) 578, 1107
RsaI GTAC 2 cut(s) 585, 797
RsaNI GTAC 2 cut(s) 584, 796
RseI CAYNNNNRTG 1 cut(s) 285
SacII CCGCGG 1 cut(s) 1044
SapI GCTCTTC 2 cut(s) 896, 1007
SaqAI TTAA 1 cut(s) 363
Sau3AI GATC 4 cut(s) 403, 578, 1107, 1228
Sau96I GGNCC 3 cut(s) 71, 173, 548
ScaI AGTACT 1 cut(s) 797
SchI GAGTC 2 cut(s) 151, 311
ScrFI CCNGG 6 cut(s) 382, 503, 551, 908, 1053, 1180
SduI GDGCHC 1 cut(s) 282
SfcI CTRYAG 2 cut(s) 1035, 1091
Sfr303I CCGCGG 1 cut(s) 1044
SfuI TTCGAA 2 cut(s) 776, 833
SgrBI CCGCGG 1 cut(s) 1044
SinI GGWCC 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 3 cut(s) 20, 482, 521
SmoI CTYRAG 3 cut(s) 20, 482, 521
Sse9I AATT 2 cut(s) 464, 538
SspI AATATT 1 cut(s) 973
StyD4I CCNGG 6 cut(s) 380, 501, 549, 906, 1051, 1178
StyI CCWWGG 1 cut(s) 456
TaaI ACNGT 5 cut(s) 347, 373, 643, 1060, 1224
TaiI ACGT 3 cut(s) 336, 342, 825
TaqI TCGA 4 cut(s) 386, 416, 776, 833
TasI AATT 2 cut(s) 464, 538
TatI WGTACW 1 cut(s) 795
TauI GCSGC 9 cut(s) 42, 95, 98, 101, 258, 938, 1020, 1043, 1046
TfiI GAWTC 6 cut(s) 409, 705, 1082, 1157, 1163, 1193
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 3 cut(s) 82, 1065, 1212
TseI GCWGC 9 cut(s) 237, 478, 752, 882, 889, 1087, 1090, 1093, 1096
Tsp45I GTSAC 3 cut(s) 82, 1065, 1212
TspDTI ATGAA 3 cut(s) 162, 499, 612
TspGWI ACGGA 4 cut(s) 186, 571, 578, 591
TspRI CASTG 1 cut(s) 376
VneI GTGCAC 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 173
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XmnI GAANNNNTTC 1 cut(s) 635
ZrmI AGTACT 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.