FvH4_5g03470

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015380
Physical Location & Seq
Reverse (-)
2062157 .. 2064917
2761 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03470.t1

Sequence Viewer

Length: 1227 bp
ATGGAAGCTTTCAGCCTTCTCAAGTACTGGCGAGGTGGCGGCCTTGTTGCTTCCACCGCTCCTCCTCTAAACTCCCCCAATGTCACCTCCGCCGCCGCCACCATTCTCACCGCCGTGGCGGAGAACTGCGACGAGAGCCATGCAACTGACGAAGACGACGACGACGGACCCTTCTTCGACTTGGAATTCTCCGCACAAGAAGCCTCCGGGAGCAACGACAGCACCAGCAGCGGCGACGAGAGTGATGAGGTGGAGGTGGAACAAGATGGGGAAGACGACGAGTCTGATGACGTCGACGGTGAAGAAGGGGAGTTTAACTTCGCTGTTTCTTCCGGGTCGAGCAATGACCGCACCGACCCGAATCTCACCATGTCTCCAACGGATGATTTGTTCTTTAAAGGCAAGCTGGTGCCGATTGAACCGGATGTGTTCAACTCGGCCGAGCCCAACTCCAAGGCTCTGTTTTCCGAGTCGCTGCTCAAGTCCGCTACCAAGCTCCGGGTCTTCATGAAGGGTCTCAAGAAGTCTAGGTCCAACTCCAACGGCCCGGAAAAGCCGGAGTCTACAAACGGATCTCAACAAACTCCCCAACAAGAACAGCCTGAGAAGGTAGAGAATAAGCAGGGGAAGCTTTTCACGGTGAAGTTGAAGGTGGAGGAAGTTCCCATTGTGTCCTTGTTCACAAGAGACAACAGCTCAAGAATCTCCACCAAAGCGGCCCAGAACAACAAGAGCCAGATTTCTGAAGAAACCGCAGCTTCTGAGGAGAAGCGGTTTTCGAAAGAGGTAGTGCAGAAGTACTTGAAAATGGTCAAGCCTCTCTACGTCCGCGTTTCGAAACGGAAGACGGTGAGCGGCCCGCAGCCGGCGGAGAATAGCCAGGCGGAGGGGGAAGTGGTTACGGAAGCGGCGCCAGCAGCGAGCAATGTGAGGAGTTTGAAGCAGGGGAATATTCCGGCGGGTTTGAGAGTGGTGTGCAAGCACTTGGGGAAGAGCCGCTCGGCTTCTTCGGCTGTAGCCGCGGCTCCTGCGGGAACTGTGCCGTCACAGAGGAGAGATGATTCGCTGCTGCAGCAGCAGGATGGGATCCAAAGCGCCATTCTGCACTGCAAGCGGTCATTCAATGCTTCCAGAGATTCCGATTATACTCCGCTATCCCGGTCAGAGAGCGATTCCTCCAACGAAAATGCGTCACAAAAACCGTCGGATCAAGGCCGAGAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

409

Amino Acids

43.98

Weight (kDa)

4.79

Isoelectric Point (pI)

57.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64080
fragaria_vesca FvH4_5g03470
malus_domestica MD06G1094800.v1.1 MD14G1115100.v1.1
prunus_persica Prupe.5G110000_v2.0.a1
pyrus_communis pycom06g09150
rosa_chinensis RchiOBHm_Chr7g0196191
rosa_laevigata RLG00000004053
rosa_multiflora Rmu_sc0007292.1_g000008
rosa_roxburghii Rroxscaffold_3G00259680
rosa_rugosa Rorug07G0032000
rosa_samantha Rh7AG157400 Rh7BG159100 Rh7CG163100 Rh7DG158400
rosa_wichuraiana Rw7G013460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 294
AccB1I GGYRCC 2 cut(s) 409, 910
AccBSI CCGCTC 3 cut(s) 59, 855, 999
AccI GTMKAC 2 cut(s) 294, 563
AccII CGCG 2 cut(s) 831, 1022
AclWI GGATC 4 cut(s) 580, 1081, 1094, 1215
AcoI YGGCCR 1 cut(s) 438
AcsI RAATTY 1 cut(s) 185
AcuI CTGAAG 1 cut(s) 765
AcyI GRCGYC 2 cut(s) 291, 911
AfaI GTAC 2 cut(s) 26, 800
AfiI CCNNNNNNNGG 3 cut(s) 498, 865, 886
AgsI TTSAA 6 cut(s) 419, 433, 649, 805, 940, 1123
AhdI GACNNNNNGTC 1 cut(s) 280
AjnI CCWGG 1 cut(s) 879
AjuI GAANNNNNNNTTGG 2 cut(s) 1083, 1115
AleI CACNNNNGTG 1 cut(s) 113
AloI GAACNNNNNNTCC 2 cut(s) 374, 406
AluBI AGCT 6 cut(s) 8, 406, 496, 631, 696, 758
AluI AGCT 6 cut(s) 8, 406, 496, 631, 696, 758
Alw26I GTCTC 3 cut(s) 378, 521, 681
AlwI GGATC 4 cut(s) 580, 1081, 1094, 1215
AlwNI CAGNNNCTG 1 cut(s) 761
AoxI GGCC 6 cut(s) 40, 438, 544, 717, 856, 1213
ApeKI GCWGC 9 cut(s) 228, 475, 755, 862, 917, 1066, 1069, 1072, 1075
ApoI RAATTY 1 cut(s) 185
Asp700I GAANNNNTTC 1 cut(s) 632
AspLEI GCGC 2 cut(s) 913, 1097
AspS9I GGNCC 5 cut(s) 167, 531, 545, 718, 857
AsuC2I CCSGG 5 cut(s) 208, 334, 500, 548, 1159
AsuHPI GGTGA 6 cut(s) 76, 100, 311, 358, 652, 862
AsuII TTCGAA 2 cut(s) 779, 836
AvaII GGWCC 2 cut(s) 167, 531
BamHI GGATCC 1 cut(s) 1086
BanI GGYRCC 2 cut(s) 409, 910
BanII GRGCYC 1 cut(s) 447
BbsI GAAGAC 4 cut(s) 159, 279, 496, 851
BbvI GCAGC 9 cut(s) 240, 462, 767, 874, 929, 1053, 1056, 1084, 1087
BccI CCATC 2 cut(s) 260, 1076
BceAI ACGGC 3 cut(s) 98, 559, 1027
BcgI CGANNNNNNTGC 2 cut(s) 122, 156
BciT130I CCWGG 1 cut(s) 881
BcnI CCSGG 5 cut(s) 208, 334, 500, 548, 1159
BcoDI GTCTC 3 cut(s) 378, 521, 681
BfaI CTAG 1 cut(s) 528
BfmI CTRYAG 2 cut(s) 1014, 1070
BfoI RGCGCY 2 cut(s) 914, 1098
BmcAI AGTACT 2 cut(s) 26, 800
Bme1390I CCNGG 6 cut(s) 208, 334, 500, 548, 881, 1159
Bme18I GGWCC 2 cut(s) 167, 531
BmeRI GACNNNNNGTC 1 cut(s) 280
BmgT120I GGNCC 5 cut(s) 167, 531, 545, 718, 857
BmiI GGNNCC 5 cut(s) 169, 411, 912, 1026, 1088
BmrFI CCNGG 6 cut(s) 208, 334, 500, 548, 881, 1159
BpiI GAAGAC 4 cut(s) 159, 279, 496, 851
BplI GAGNNNNNCTC 2 cut(s) 434, 466
Bpu14I TTCGAA 2 cut(s) 779, 836
BpuEI CTTGAG 4 cut(s) 5, 464, 503, 682
BpuMI CCSGG 5 cut(s) 208, 334, 500, 548, 1159
BsaHI GRCGYC 2 cut(s) 291, 911
BsaI GGTCTC 1 cut(s) 521
BsaJI CCNNGG 3 cut(s) 114, 453, 1020
BsaWI WCCGGW 1 cut(s) 421
BsaXI ACNNNNNCTCC 6 cut(s) 46, 76, 358, 388, 758, 788
Bsc4I CCNNNNNNNGG 3 cut(s) 498, 865, 886
Bse118I RCCGGY 1 cut(s) 865
Bse1I ACTGG 1 cut(s) 32
Bse3DI GCAATG 2 cut(s) 349, 931
BseBI CCWGG 1 cut(s) 881
BseDI CCNNGG 3 cut(s) 114, 453, 1020
BseGI GGATG 3 cut(s) 388, 430, 1087
BseLI CCNNNNNNNGG 3 cut(s) 498, 865, 886
BseMI GCAATG 2 cut(s) 349, 931
BseMII CTCAG 2 cut(s) 594, 753
BseNI ACTGG 1 cut(s) 32
BseRI GAGGAG 5 cut(s) 51, 54, 779, 946, 1066
BseX3I CGGCCG 1 cut(s) 438
BseXI GCAGC 9 cut(s) 240, 462, 767, 874, 929, 1053, 1056, 1084, 1087
BsgI GTGCAG 2 cut(s) 812, 1088
Bsh1236I CGCG 2 cut(s) 831, 1022
Bsh1285I CGRYCG 1 cut(s) 441
BshFI GGCC 6 cut(s) 42, 440, 546, 719, 858, 1215
BshNI GGYRCC 2 cut(s) 409, 910
BsiEI CGRYCG 1 cut(s) 441
BsiSI CCGG 9 cut(s) 207, 333, 422, 499, 548, 557, 866, 956, 1159
BslI CCNNNNNNNGG 3 cut(s) 498, 865, 886
BsmAI GTCTC 3 cut(s) 378, 521, 681
BsnI GGCC 6 cut(s) 42, 440, 546, 719, 858, 1215
Bso31I GGTCTC 1 cut(s) 521
Bsp119I TTCGAA 2 cut(s) 779, 836
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 3 cut(s) 572, 1086, 1207
BspANI GGCC 6 cut(s) 42, 440, 546, 719, 858, 1215
BspCNI CTCAG 2 cut(s) 595, 754
BspFNI CGCG 2 cut(s) 831, 1022
BspHI TCATGA 1 cut(s) 507
BspLI GGNNCC 5 cut(s) 169, 411, 912, 1026, 1088
BspMAI CTGCAG 1 cut(s) 1074
BspPI GGATC 4 cut(s) 580, 1081, 1094, 1215
BspQI GCTCTTC 1 cut(s) 986
BspT104I TTCGAA 2 cut(s) 779, 836
BspT107I GGYRCC 2 cut(s) 409, 910
BspTNI GGTCTC 1 cut(s) 521
BsrBI CCGCTC 3 cut(s) 59, 855, 999
BsrDI GCAATG 2 cut(s) 349, 931
BsrFI RCCGGY 1 cut(s) 865
BsrI ACTGG 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 865
BssECI CCNNGG 3 cut(s) 114, 453, 1020
BssMI GATC 3 cut(s) 572, 1086, 1207
BssNI GRCGYC 2 cut(s) 291, 911
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 881
Bst4CI ACNGT 5 cut(s) 299, 640, 850, 1039, 1203
Bst6I CTCTTC 1 cut(s) 986
BstACI GRCGYC 2 cut(s) 291, 911
BstBI TTCGAA 2 cut(s) 779, 836
BstC8I GCNNGC 7 cut(s) 404, 860, 867, 915, 922, 980, 1112
BstDEI CTNAG 2 cut(s) 603, 762
BstDSI CCRYGG 2 cut(s) 114, 1020
BstF5I GGATG 3 cut(s) 388, 430, 1087
BstFNI CGCG 2 cut(s) 831, 1022
BstH2I RGCGCY 2 cut(s) 914, 1098
BstHHI GCGC 2 cut(s) 913, 1097
BstKTI GATC 3 cut(s) 575, 1089, 1210
BstMAI GTCTC 3 cut(s) 378, 521, 681
BstMBI GATC 3 cut(s) 572, 1086, 1207
BstMCI CGRYCG 1 cut(s) 441
BstNI CCWGG 1 cut(s) 881
BstSCI CCNGG 6 cut(s) 206, 332, 498, 546, 879, 1157
BstSFI CTRYAG 2 cut(s) 1014, 1070
BstUI CGCG 2 cut(s) 831, 1022
BstV1I GCAGC 9 cut(s) 240, 462, 767, 874, 929, 1053, 1056, 1084, 1087
BstV2I GAAGAC 4 cut(s) 159, 279, 496, 851
BstX2I RGATCY 2 cut(s) 572, 1086
BstYI RGATCY 2 cut(s) 572, 1086
BstZI CGGCCG 1 cut(s) 438
BsuRI GGCC 6 cut(s) 42, 440, 546, 719, 858, 1215
BtgI CCRYGG 2 cut(s) 114, 1020
BtsCI GGATG 3 cut(s) 388, 430, 1087
BtsI GCAGTG 1 cut(s) 1105
BtsIMutI CAGTG 1 cut(s) 1105
Cac8I GCNNGC 7 cut(s) 404, 860, 867, 915, 922, 980, 1112
CaiI CAGNNNCTG 1 cut(s) 761
CciI TCATGA 1 cut(s) 507
CfoI GCGC 2 cut(s) 913, 1097
Cfr10I RCCGGY 1 cut(s) 865
Cfr13I GGNCC 5 cut(s) 167, 531, 545, 718, 857
Cfr42I CCGCGG 1 cut(s) 1023
CseI GACGC 1 cut(s) 1179
Csp6I GTAC 2 cut(s) 25, 799
CviAII CATG 3 cut(s) 140, 370, 508
CviQI GTAC 2 cut(s) 25, 799
DdeI CTNAG 2 cut(s) 603, 762
DinI GGCGCC 1 cut(s) 912
DpnI GATC 3 cut(s) 574, 1088, 1209
DpnII GATC 3 cut(s) 572, 1086, 1207
DraI TTTAAA 1 cut(s) 397
DriI GACNNNNNGTC 1 cut(s) 280
EaeI YGGCCR 1 cut(s) 438
EagI CGGCCG 1 cut(s) 438
Eam1104I CTCTTC 1 cut(s) 986
Eam1105I GACNNNNNGTC 1 cut(s) 280
EarI CTCTTC 1 cut(s) 986
EciI GGCGGA 4 cut(s) 79, 134, 884, 899
EclXI CGGCCG 1 cut(s) 438
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 1 cut(s) 447
Eco31I GGTCTC 1 cut(s) 521
Eco47I GGWCC 2 cut(s) 167, 531
Eco52I CGGCCG 1 cut(s) 438
Eco57I CTGAAG 1 cut(s) 765
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 1 cut(s) 879
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 1 cut(s) 447
EgeI GGCGCC 1 cut(s) 912
EheI GGCGCC 1 cut(s) 912
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 3 cut(s) 143, 373, 511
FaiI YATR 4 cut(s) 141, 371, 509, 1146
FatI CATG 3 cut(s) 139, 369, 507
FauI CCCGC 3 cut(s) 867, 952, 1024
FblI GTMKAC 2 cut(s) 294, 563
FokI GGATG 3 cut(s) 395, 437, 1094
FriOI GRGCYC 1 cut(s) 447
FspBI CTAG 1 cut(s) 528
GlaI GCGC 2 cut(s) 912, 1096
HaeII RGCGCY 2 cut(s) 914, 1098
HaeIII GGCC 6 cut(s) 42, 440, 546, 719, 858, 1215
HapII CCGG 9 cut(s) 207, 333, 422, 499, 548, 557, 866, 956, 1159
HgaI GACGC 1 cut(s) 1179
HhaI GCGC 2 cut(s) 913, 1097
Hin1I GRCGYC 2 cut(s) 291, 911
Hin1II CATG 3 cut(s) 143, 373, 511
Hin6I GCGC 2 cut(s) 911, 1095
HinP1I GCGC 2 cut(s) 911, 1095
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 2 cut(s) 6, 629
HinfI GANTC 8 cut(s) 281, 361, 470, 560, 702, 1061, 1136, 1172
HpaII CCGG 9 cut(s) 207, 333, 422, 499, 548, 557, 866, 956, 1159
HphI GGTGA 6 cut(s) 76, 100, 311, 358, 652, 862
Hpy166II GTNNAC 3 cut(s) 295, 564, 681
Hpy188I TCNGA 7 cut(s) 286, 469, 745, 763, 1141, 1165, 1207
Hpy188III TCNNGA 4 cut(s) 508, 520, 699, 1131
Hpy8I GTNNAC 3 cut(s) 295, 564, 681
Hpy99I CGWCG 9 cut(s) 134, 161, 164, 167, 239, 281, 296, 299, 1207
HpyAV CCTTC 6 cut(s) 26, 181, 299, 505, 601, 643
HpyCH4III ACNGT 5 cut(s) 299, 640, 850, 1039, 1203
HpyCH4IV ACGT 2 cut(s) 291, 825
HpyCH4V TGCA 6 cut(s) 143, 793, 978, 1072, 1105, 1110
HpyF3I CTNAG 2 cut(s) 603, 762
HpySE526I ACGT 2 cut(s) 291, 825
Hsp92I GRCGYC 2 cut(s) 291, 911
Hsp92II CATG 3 cut(s) 143, 373, 511
HspAI GCGC 2 cut(s) 911, 1095
KasI GGCGCC 1 cut(s) 910
KroI GCCGGC 1 cut(s) 865
KroNI GCCGGC 1 cut(s) 867
KspI CCGCGG 1 cut(s) 1023
Kzo9I GATC 3 cut(s) 572, 1086, 1207
LguI GCTCTTC 1 cut(s) 986
LmnI GCTCC 4 cut(s) 64, 210, 501, 1030
Lsp1109I GCAGC 9 cut(s) 240, 462, 767, 874, 929, 1053, 1056, 1084, 1087
MaeI CTAG 1 cut(s) 528
MaeII ACGT 2 cut(s) 291, 825
MaeIII GTNAC 4 cut(s) 82, 898, 1044, 1191
MalI GATC 3 cut(s) 574, 1088, 1209
MbiI CCGCTC 3 cut(s) 59, 855, 999
MboI GATC 3 cut(s) 572, 1086, 1207
MflI RGATCY 2 cut(s) 572, 1086
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 1 cut(s) 185
Mly113I GGCGCC 1 cut(s) 911
MlyI GAGTC 3 cut(s) 290, 479, 569
MmeI TCCRAC 5 cut(s) 401, 558, 564, 1185, 1203
MroNI GCCGGC 1 cut(s) 865
MroXI GAANNNNTTC 1 cut(s) 632
MseI TTAA 2 cut(s) 315, 396
MslI CAYNNNNRTG 1 cut(s) 113
MspA1I CMGCKG 2 cut(s) 231, 1022
MspI CCGG 9 cut(s) 207, 333, 422, 499, 548, 557, 866, 956, 1159
MspR9I CCNGG 6 cut(s) 208, 334, 500, 548, 881, 1159
MvaI CCWGG 1 cut(s) 881
MvnI CGCG 2 cut(s) 831, 1022
NaeI GCCGGC 1 cut(s) 867
NarI GGCGCC 1 cut(s) 911
NciI CCSGG 5 cut(s) 208, 334, 500, 548, 1159
NdeII GATC 3 cut(s) 572, 1086, 1207
NgoMIV GCCGGC 1 cut(s) 865
NlaIII CATG 3 cut(s) 143, 373, 511
NlaIV GGNNCC 5 cut(s) 169, 411, 912, 1026, 1088
NmeAIII GCCGAG 3 cut(s) 416, 466, 980
NmuCI GTSAC 3 cut(s) 82, 1044, 1191
NspV TTCGAA 2 cut(s) 779, 836
OliI CACNNNNGTG 1 cut(s) 113
PagI TCATGA 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 986
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PdiI GCCGGC 1 cut(s) 867
PdmI GAANNNNTTC 1 cut(s) 632
PfeI GAWTC 5 cut(s) 361, 702, 1061, 1136, 1172
PfoI TCCNGGA 1 cut(s) 206
PleI GAGTC 3 cut(s) 289, 478, 568
PluTI GGCGCC 1 cut(s) 914
PpsI GAGTC 3 cut(s) 289, 478, 568
Psp6I CCWGG 1 cut(s) 879
PspGI CCWGG 1 cut(s) 879
PspN4I GGNNCC 5 cut(s) 169, 411, 912, 1026, 1088
PspPI GGNCC 5 cut(s) 167, 531, 545, 718, 857
PstI CTGCAG 1 cut(s) 1074
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 2 cut(s) 572, 1086
RsaI GTAC 2 cut(s) 26, 800
RsaNI GTAC 2 cut(s) 25, 799
RseI CAYNNNNRTG 1 cut(s) 113
SacII CCGCGG 1 cut(s) 1023
SalI GTCGAC 1 cut(s) 293
SapI GCTCTTC 1 cut(s) 986
SaqAI TTAA 2 cut(s) 315, 396
Sau3AI GATC 3 cut(s) 572, 1086, 1207
Sau96I GGNCC 5 cut(s) 167, 531, 545, 718, 857
ScaI AGTACT 2 cut(s) 26, 800
SchI GAGTC 3 cut(s) 290, 479, 569
ScrFI CCNGG 6 cut(s) 208, 334, 500, 548, 881, 1159
SduI GDGCHC 1 cut(s) 447
SfcI CTRYAG 2 cut(s) 1014, 1070
SfoI GGCGCC 1 cut(s) 912
Sfr303I CCGCGG 1 cut(s) 1023
SfuI TTCGAA 2 cut(s) 779, 836
SgrBI CCGCGG 1 cut(s) 1023
SgrDI CGTCGACG 1 cut(s) 293
SinI GGWCC 2 cut(s) 167, 531
SmiMI CAYNNNNRTG 1 cut(s) 113
SmlI CTYRAG 4 cut(s) 20, 479, 518, 697
SmoI CTYRAG 4 cut(s) 20, 479, 518, 697
Sse9I AATT 1 cut(s) 185
SspDI GGCGCC 1 cut(s) 910
SspI AATATT 1 cut(s) 952
SspMI CTAG 1 cut(s) 528
StyD4I CCNGG 6 cut(s) 206, 332, 498, 546, 879, 1157
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 5 cut(s) 299, 640, 850, 1039, 1203
TaiI ACGT 2 cut(s) 294, 828
TaqI TCGA 5 cut(s) 177, 294, 338, 779, 836
TasI AATT 1 cut(s) 185
TatI WGTACW 2 cut(s) 24, 798
TfiI GAWTC 5 cut(s) 361, 702, 1061, 1136, 1172
Tru1I TTAA 2 cut(s) 315, 396
Tru9I TTAA 2 cut(s) 315, 396
TscAI CASTG 1 cut(s) 1112
TseFI GTSAC 3 cut(s) 82, 1044, 1191
TseI GCWGC 9 cut(s) 228, 475, 755, 862, 917, 1066, 1069, 1072, 1075
Tsp45I GTSAC 3 cut(s) 82, 1044, 1191
TspDTI ATGAA 2 cut(s) 496, 524
TspGWI ACGGA 5 cut(s) 180, 395, 585, 856, 917
TspRI CASTG 1 cut(s) 1112
VpaK11BI GGWCC 2 cut(s) 167, 531
XapI RAATTY 1 cut(s) 185
XmiI GTMKAC 2 cut(s) 294, 563
XmnI GAANNNNTTC 1 cut(s) 632
XspI CTAG 1 cut(s) 528
ZraI GACGTC 1 cut(s) 292
ZrmI AGTACT 2 cut(s) 26, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.