RLG00000004053

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015380
Physical Location & Seq
Reverse (-)
55686248 .. 55687702
1455 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004053

Sequence Viewer

Length: 1284 bp
ATGGAAGCTTTTAGCCTGCTCAAGTATTGGCGAGGTGGCGGCCTTGTTGCCTCCTCCGCTCCTCCTCCTAATGGCCCTAACAATATCACCTCCGCCGCCGCCACCATCCTCACCGCGGTCGCTGAGAACAGCGACGAGAGTCATGAAACTGACGACGACGATGACGACGACGGACCTTTCTTTGACTTGGAGTTTGCCGTCCCTGACGAGGCCGAAGCCCACGAAGCCTCTGGAAGCAGCGACAGCACCACCAGCGGCGACGAGAGTGAGGTGGAAGTGCACCAAGATGGGGAAGATGATGAGTCTGATGATGATGACGTGGACGTGGACGGTGAGGAGGGTGAGTTTAACTTCACTGTGTCTTCCGGGTCGAGCAATGACCGCACCGACCCGAATCTGTCGATGTCTCCCTCTGACGATTTGTTCTTCAAGGGTAAGCTGGTGCCGATTGAGCCTTCCTCCATTGTGTTCAACCCGTCCGAGCCGAACTCCAAGGCTCAATTCGCTGGGTCGCTGCTCAAGTCCGCCACCAAGTTCCGGGTCTTCATGTCGGGTCTCAAGAAATCCAAGTCCAATTCCAACGGCCCGGAAAACAAAACGGAGCCTACAAACGGATCTGTACAAGAACAACATGAAAAACAAGAGAATAAGCAACAGAAGCAGGGGAAGCTTTTCACGGTGAAGTTGAAGGTGGAGGAAGTTCCCATTGTGTCCTTGTTCACCAGAGACAACAGCTCCAGAATCTCCACAAAGACCCAGAACCAGAAAACCCAGAGTTCCGAAGAAGCTGCTTCTGAGGAGAAGCGCTTTTCGAAGGAGGTAATGCAGAAGTACTTGAAAATGGTGAAGCCTCTCTACGTCCGCGTTTCCAAACGCTACGGCGAGAAGACGAGGCTTTCCGGTCAACTCAGCGTAAGCAGCCCGCAGCCGGCAGAGAAGAGCCAGGCGGAGGCGGAAGTTTCGGAACCGGCGGCGAGCAATGCGAAGAGTACTTTGAAACAGGGGAATATTCCGGCGGGTTTGAGAGTTGTTTGCAAGCACTTGGGGAAGAGCCGCTCGGCTTCTTCGGCTGTAGCCGCGGCTCCACCGGGAACTGTGCCATCACAGAGGAGAGATGATTCGCTGCTGCAGCAGCAGGATGGGATCCAGAGCGCCATTCTCCATTGCAAGCGGTCATTCAATGCTTCCAGAGAATCCGATTCTACTCCGCTATCTCGGTCGGTGAGCGATTCCTGCAACGAGAAGGGGTCACGAAAACCGTCGGATCAAGGCGGAGAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

428

Amino Acids

46.05

Weight (kDa)

4.88

Isoelectric Point (pI)

55.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64080
fragaria_vesca FvH4_5g03470
malus_domestica MD06G1094800.v1.1 MD14G1115100.v1.1
prunus_persica Prupe.5G110000_v2.0.a1
pyrus_communis pycom06g09150
rosa_chinensis RchiOBHm_Chr7g0196191
rosa_laevigata RLG00000004053
rosa_multiflora Rmu_sc0007292.1_g000008
rosa_roxburghii Rroxscaffold_3G00259680
rosa_rugosa Rorug07G0032000
rosa_samantha Rh7AG157400 Rh7BG159100 Rh7CG163100 Rh7DG158400
rosa_wichuraiana Rw7G013460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 442
AccBSI CCGCTC 2 cut(s) 59, 1056
AccII CGCG 3 cut(s) 116, 864, 1079
AclWI GGATC 4 cut(s) 622, 1138, 1151, 1272
AfaI GTAC 3 cut(s) 621, 833, 991
AfeI AGCGCT 1 cut(s) 806
AfiI CCNNNNNNNGG 8 cut(s) 71, 115, 208, 289, 537, 611, 928, 949
AgsI TTSAA 6 cut(s) 430, 472, 688, 838, 997, 1180
AjiI CACGTC 2 cut(s) 319, 325
AjnI CCWGG 1 cut(s) 942
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
AluBI AGCT 5 cut(s) 8, 439, 670, 735, 788
AluI AGCT 5 cut(s) 8, 439, 670, 735, 788
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 3 cut(s) 411, 560, 720
Alw44I GTGCAC 1 cut(s) 278
AlwI GGATC 4 cut(s) 622, 1138, 1151, 1272
Aor51HI AGCGCT 1 cut(s) 806
AoxI GGCC 4 cut(s) 40, 73, 210, 583
ApaLI GTGCAC 1 cut(s) 278
ApeKI GCWGC 9 cut(s) 237, 514, 788, 918, 925, 1123, 1126, 1129, 1132
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
Asp700I GAANNNNTTC 1 cut(s) 671
AspLEI GCGC 2 cut(s) 807, 1154
AspS9I GGNCC 3 cut(s) 74, 173, 584
AsuC2I CCSGG 4 cut(s) 367, 539, 587, 1089
AsuHPI GGTGA 8 cut(s) 79, 103, 344, 353, 691, 712, 856, 1234
AsuII TTCGAA 1 cut(s) 812
AvaII GGWCC 1 cut(s) 173
BaeGI GKGCMC 1 cut(s) 282
BamHI GGATCC 1 cut(s) 1143
BanI GGYRCC 1 cut(s) 442
BarI GAAGNNNNNNTAC 2 cut(s) 839, 871
BbsI GAAGAC 3 cut(s) 354, 535, 893
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 9 cut(s) 249, 501, 775, 930, 937, 1110, 1113, 1141, 1144
BccI CCATC 4 cut(s) 113, 281, 1108, 1133
BceAI ACGGC 3 cut(s) 182, 598, 895
BcgI CGANNNNNNTGC 2 cut(s) 770, 804
BciT130I CCWGG 1 cut(s) 944
BcnI CCSGG 4 cut(s) 367, 539, 587, 1089
BcoDI GTCTC 3 cut(s) 411, 560, 720
BfmI CTRYAG 2 cut(s) 1071, 1127
BfoI RGCGCY 2 cut(s) 808, 1155
BmcAI AGTACT 2 cut(s) 833, 991
Bme1390I CCNGG 5 cut(s) 367, 539, 587, 944, 1089
Bme18I GGWCC 1 cut(s) 173
BmgBI CACGTC 2 cut(s) 319, 325
BmgT120I GGNCC 3 cut(s) 74, 173, 584
BmiI GGNNCC 5 cut(s) 444, 603, 966, 1083, 1145
BmrFI CCNGG 5 cut(s) 367, 539, 587, 944, 1089
BoxI GACNNNNGTC 1 cut(s) 138
BpiI GAAGAC 3 cut(s) 354, 535, 893
BplI GAGNNNNNCTC 4 cut(s) 443, 475, 473, 505
BpmI CTGGAG 1 cut(s) 721
Bpu14I TTCGAA 1 cut(s) 812
BpuEI CTTGAG 3 cut(s) 5, 503, 542
BpuMI CCSGG 4 cut(s) 367, 539, 587, 1089
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 3 cut(s) 114, 492, 1077
BsaWI WCCGGW 1 cut(s) 899
BsaXI ACNNNNNCTCC 4 cut(s) 329, 359, 719, 749
Bsc4I CCNNNNNNNGG 8 cut(s) 71, 115, 208, 289, 537, 611, 928, 949
Bse118I RCCGGY 2 cut(s) 928, 967
Bse3DI GCAATG 3 cut(s) 382, 985, 1162
BseBI CCWGG 1 cut(s) 944
BseDI CCNNGG 3 cut(s) 114, 492, 1077
BseGI GGATG 2 cut(s) 105, 1144
BseLI CCNNNNNNNGG 8 cut(s) 71, 115, 208, 289, 537, 611, 928, 949
BseMI GCAATG 3 cut(s) 382, 985, 1162
BseMII CTCAG 3 cut(s) 114, 786, 922
BseRI GAGGAG 6 cut(s) 43, 51, 54, 350, 812, 1123
BseSI GKGCMC 1 cut(s) 282
BseXI GCAGC 9 cut(s) 249, 501, 775, 930, 937, 1110, 1113, 1141, 1144
BseYI CCCAGC 1 cut(s) 506
Bsh1236I CGCG 3 cut(s) 116, 864, 1079
Bsh1285I CGRYCG 2 cut(s) 120, 1220
BshFI GGCC 4 cut(s) 42, 75, 212, 585
BshNI GGYRCC 1 cut(s) 442
BsiEI CGRYCG 2 cut(s) 120, 1220
BsiHKAI GWGCWC 1 cut(s) 282
BsiSI CCGG 8 cut(s) 366, 538, 587, 900, 929, 968, 1013, 1088
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 8 cut(s) 71, 115, 208, 289, 537, 611, 928, 949
BsmAI GTCTC 3 cut(s) 411, 560, 720
BsmFI GGGAC 1 cut(s) 185
BsnI GGCC 4 cut(s) 42, 75, 212, 585
Bso31I GGTCTC 1 cut(s) 560
Bsp119I TTCGAA 1 cut(s) 812
Bsp1286I GDGCHC 1 cut(s) 282
Bsp1407I TGTACA 1 cut(s) 619
Bsp143I GATC 3 cut(s) 614, 1143, 1264
BspANI GGCC 4 cut(s) 42, 75, 212, 585
BspCNI CTCAG 3 cut(s) 115, 787, 921
BspFNI CGCG 3 cut(s) 116, 864, 1079
BspHI TCATGA 1 cut(s) 142
BspLI GGNNCC 5 cut(s) 444, 603, 966, 1083, 1145
BspMAI CTGCAG 1 cut(s) 1131
BspPI GGATC 4 cut(s) 622, 1138, 1151, 1272
BspQI GCTCTTC 2 cut(s) 932, 1043
BspT104I TTCGAA 1 cut(s) 812
BspT107I GGYRCC 1 cut(s) 442
BspTNI GGTCTC 1 cut(s) 560
BsrBI CCGCTC 2 cut(s) 59, 1056
BsrDI GCAATG 3 cut(s) 382, 985, 1162
BsrFI RCCGGY 2 cut(s) 928, 967
BsrGI TGTACA 1 cut(s) 619
BssAI RCCGGY 2 cut(s) 928, 967
BssECI CCNNGG 3 cut(s) 114, 492, 1077
BssMI GATC 3 cut(s) 614, 1143, 1264
BssT1I CCWWGG 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 944
Bst4CI ACNGT 5 cut(s) 332, 358, 679, 1096, 1260
Bst6I CTCTTC 3 cut(s) 932, 980, 1043
BstAUI TGTACA 1 cut(s) 619
BstBI TTCGAA 1 cut(s) 812
BstC8I GCNNGC 6 cut(s) 17, 923, 930, 976, 1037, 1169
BstDEI CTNAG 3 cut(s) 123, 795, 908
BstDSI CCRYGG 2 cut(s) 114, 1077
BstF5I GGATG 2 cut(s) 105, 1144
BstFNI CGCG 3 cut(s) 116, 864, 1079
BstH2I RGCGCY 2 cut(s) 808, 1155
BstHHI GCGC 2 cut(s) 807, 1154
BstKTI GATC 3 cut(s) 617, 1146, 1267
BstMAI GTCTC 3 cut(s) 411, 560, 720
BstMBI GATC 3 cut(s) 614, 1143, 1264
BstMCI CGRYCG 2 cut(s) 120, 1220
BstNI CCWGG 1 cut(s) 944
BstPAI GACNNNNGTC 1 cut(s) 138
BstSCI CCNGG 5 cut(s) 365, 537, 585, 942, 1087
BstSFI CTRYAG 2 cut(s) 1071, 1127
BstSLI GKGCMC 1 cut(s) 282
BstUI CGCG 3 cut(s) 116, 864, 1079
BstV1I GCAGC 9 cut(s) 249, 501, 775, 930, 937, 1110, 1113, 1141, 1144
BstV2I GAAGAC 3 cut(s) 354, 535, 893
BstX2I RGATCY 2 cut(s) 614, 1143
BstYI RGATCY 2 cut(s) 614, 1143
BsuRI GGCC 4 cut(s) 42, 75, 212, 585
BtgI CCRYGG 2 cut(s) 114, 1077
BtrI CACGTC 2 cut(s) 319, 325
BtsCI GGATG 2 cut(s) 105, 1144
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 6 cut(s) 17, 923, 930, 976, 1037, 1169
CciI TCATGA 1 cut(s) 142
CfoI GCGC 2 cut(s) 807, 1154
Cfr10I RCCGGY 2 cut(s) 928, 967
Cfr13I GGNCC 3 cut(s) 74, 173, 584
Cfr42I CCGCGG 2 cut(s) 117, 1080
Csp6I GTAC 3 cut(s) 620, 832, 990
CviAII CATG 3 cut(s) 143, 547, 632
CviQI GTAC 3 cut(s) 620, 832, 990
DdeI CTNAG 3 cut(s) 123, 795, 908
DpnI GATC 3 cut(s) 616, 1145, 1266
DpnII GATC 3 cut(s) 614, 1143, 1264
Eam1104I CTCTTC 3 cut(s) 932, 980, 1043
EarI CTCTTC 3 cut(s) 932, 980, 1043
EciI GGCGGA 4 cut(s) 82, 514, 962, 968
Eco130I CCWWGG 1 cut(s) 492
Eco31I GGTCTC 1 cut(s) 560
Eco47I GGWCC 1 cut(s) 173
Eco47III AGCGCT 1 cut(s) 806
EcoRII CCWGG 1 cut(s) 942
EcoT14I CCWWGG 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 3 cut(s) 146, 550, 635
FaiI YATR 3 cut(s) 144, 548, 633
FaqI GGGAC 1 cut(s) 185
FatI CATG 3 cut(s) 142, 546, 631
FauI CCCGC 2 cut(s) 930, 1009
FokI GGATG 2 cut(s) 92, 1151
GlaI GCGC 2 cut(s) 806, 1153
GsaI CCCAGC 1 cut(s) 510
GsuI CTGGAG 1 cut(s) 721
HaeII RGCGCY 2 cut(s) 808, 1155
HaeIII GGCC 4 cut(s) 42, 75, 212, 585
HapII CCGG 8 cut(s) 366, 538, 587, 900, 929, 968, 1013, 1088
HhaI GCGC 2 cut(s) 807, 1154
Hin1II CATG 3 cut(s) 146, 550, 635
Hin6I GCGC 2 cut(s) 805, 1152
HinP1I GCGC 2 cut(s) 805, 1152
HincII GTYRAC 1 cut(s) 905
HindII GTYRAC 1 cut(s) 905
HindIII AAGCTT 2 cut(s) 6, 668
HinfI GANTC 8 cut(s) 139, 302, 394, 741, 1118, 1193, 1199, 1229
HpaII CCGG 8 cut(s) 366, 538, 587, 900, 929, 968, 1013, 1088
HphI GGTGA 8 cut(s) 79, 103, 344, 353, 691, 712, 856, 1234
Hpy166II GTNNAC 5 cut(s) 280, 322, 328, 720, 905
Hpy188I TCNGA 8 cut(s) 307, 415, 481, 781, 796, 964, 1198, 1264
Hpy188III TCNNGA 7 cut(s) 143, 231, 559, 738, 1147, 1188, 1251
Hpy8I GTNNAC 5 cut(s) 280, 322, 328, 720, 905
Hpy99I CGWCG 7 cut(s) 137, 158, 161, 170, 173, 263, 1264
HpyAV CCTTC 4 cut(s) 465, 682, 808, 1237
HpyCH4III ACNGT 5 cut(s) 332, 358, 679, 1096, 1260
HpyCH4IV ACGT 3 cut(s) 318, 324, 858
HpyCH4V TGCA 6 cut(s) 280, 826, 1035, 1129, 1167, 1236
HpyF3I CTNAG 3 cut(s) 123, 795, 908
HpySE526I ACGT 3 cut(s) 318, 324, 858
Hsp92II CATG 3 cut(s) 146, 550, 635
HspAI GCGC 2 cut(s) 805, 1152
KroI GCCGGC 1 cut(s) 928
KroNI GCCGGC 1 cut(s) 930
KspI CCGCGG 2 cut(s) 117, 1080
Kzo9I GATC 3 cut(s) 614, 1143, 1264
LguI GCTCTTC 2 cut(s) 932, 1043
LmnI GCTCC 4 cut(s) 64, 601, 740, 1087
Lsp1109I GCAGC 9 cut(s) 249, 501, 775, 930, 937, 1110, 1113, 1141, 1144
MaeII ACGT 3 cut(s) 318, 324, 858
MaeIII GTNAC 1 cut(s) 1248
MalI GATC 3 cut(s) 616, 1145, 1266
MbiI CCGCTC 2 cut(s) 59, 1056
MboI GATC 3 cut(s) 614, 1143, 1264
MflI RGATCY 2 cut(s) 614, 1143
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 2 cut(s) 500, 574
MlyI GAGTC 2 cut(s) 148, 311
MmeI TCCRAC 2 cut(s) 603, 1242
MroNI GCCGGC 1 cut(s) 928
MroXI GAANNNNTTC 1 cut(s) 671
MseI TTAA 1 cut(s) 348
MslI CAYNNNNRTG 1 cut(s) 285
MspA1I CMGCKG 3 cut(s) 116, 255, 1079
MspI CCGG 8 cut(s) 366, 538, 587, 900, 929, 968, 1013, 1088
MspR9I CCNGG 5 cut(s) 367, 539, 587, 944, 1089
MvaI CCWGG 1 cut(s) 944
MvnI CGCG 3 cut(s) 116, 864, 1079
NaeI GCCGGC 1 cut(s) 930
NciI CCSGG 4 cut(s) 367, 539, 587, 1089
NdeII GATC 3 cut(s) 614, 1143, 1264
NgoMIV GCCGGC 1 cut(s) 928
NlaIII CATG 3 cut(s) 146, 550, 635
NlaIV GGNNCC 5 cut(s) 444, 603, 966, 1083, 1145
NmeAIII GCCGAG 1 cut(s) 1037
NmuCI GTSAC 1 cut(s) 1248
NspV TTCGAA 1 cut(s) 812
PagI TCATGA 1 cut(s) 142
PciSI GCTCTTC 2 cut(s) 932, 1043
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 165
PdiI GCCGGC 1 cut(s) 930
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 6 cut(s) 394, 741, 1118, 1193, 1199, 1229
PleI GAGTC 2 cut(s) 147, 310
PpsI GAGTC 2 cut(s) 147, 310
PshAI GACNNNNGTC 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 942
PspFI CCCAGC 1 cut(s) 506
PspGI CCWGG 1 cut(s) 942
PspN4I GGNNCC 5 cut(s) 444, 603, 966, 1083, 1145
PspPI GGNCC 3 cut(s) 74, 173, 584
PstI CTGCAG 1 cut(s) 1131
PsuI RGATCY 2 cut(s) 614, 1143
RsaI GTAC 3 cut(s) 621, 833, 991
RsaNI GTAC 3 cut(s) 620, 832, 990
RseI CAYNNNNRTG 1 cut(s) 285
SacII CCGCGG 2 cut(s) 117, 1080
SapI GCTCTTC 2 cut(s) 932, 1043
SaqAI TTAA 1 cut(s) 348
Sau3AI GATC 3 cut(s) 614, 1143, 1264
Sau96I GGNCC 3 cut(s) 74, 173, 584
ScaI AGTACT 2 cut(s) 833, 991
SchI GAGTC 2 cut(s) 148, 311
ScrFI CCNGG 5 cut(s) 367, 539, 587, 944, 1089
SduI GDGCHC 1 cut(s) 282
SfcI CTRYAG 2 cut(s) 1071, 1127
Sfr303I CCGCGG 2 cut(s) 117, 1080
SfuI TTCGAA 1 cut(s) 812
SgrBI CCGCGG 2 cut(s) 117, 1080
SinI GGWCC 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 3 cut(s) 20, 518, 557
SmoI CTYRAG 3 cut(s) 20, 518, 557
Sse9I AATT 2 cut(s) 500, 574
SspI AATATT 1 cut(s) 1009
StyD4I CCNGG 5 cut(s) 365, 537, 585, 942, 1087
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 5 cut(s) 332, 358, 679, 1096, 1260
TaiI ACGT 3 cut(s) 321, 327, 861
TaqI TCGA 3 cut(s) 371, 401, 812
TaqII GACCGA 1 cut(s) 1206
TasI AATT 2 cut(s) 500, 574
TatI WGTACW 3 cut(s) 619, 831, 989
TauI GCSGC 8 cut(s) 42, 98, 101, 258, 974, 1056, 1079, 1082
TfiI GAWTC 6 cut(s) 394, 741, 1118, 1193, 1199, 1229
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 1 cut(s) 1248
TseI GCWGC 9 cut(s) 237, 514, 788, 918, 925, 1123, 1126, 1129, 1132
Tsp45I GTSAC 1 cut(s) 1248
TspDTI ATGAA 3 cut(s) 159, 535, 648
TspGWI ACGGA 3 cut(s) 186, 614, 627
TspRI CASTG 1 cut(s) 361
VneI GTGCAC 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 173
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XmnI GAANNNNTTC 1 cut(s) 671
ZrmI AGTACT 2 cut(s) 833, 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.