MD06G1094800.v1.1

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015380
Physical Location & Seq
Reverse (-)
22745251 .. 22747463
2213 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1094800.v1.1.491

Sequence Viewer

Length: 1530 bp
ATGGAAGCTTTCAGCTTGCTCAAGTACTGGCGAGGCGGTGGTGCTGTGTATGGCACCCCCCTTCCTAATTGCCCCGACACTCCTGATTCTGCTAAAATGCGCACCCCCACCACCACTTCACCCACCACCACGATCCTCACCGCCGTCGCCCAAAATGACGACGAAGACACCGACGACGACGACGGCCCTTTCTTCGACTTGGAGTTTGCCGTCCCTGACGAAGATGAAGCGGCCGCCGCCCAAACCCACCAGCCCTGTGGAGACGGAGATTGTACGGACGACAGCGACGAGGAGGCCCGACTAGAAGACGACGACGGCGATGACGAGTCTGACGATTGTGATGATGGAGAGGGGGAGTTCAACTTCACTGTCTCTTCCGGGTCGAGCCAGGACCGTACGGACGACCCGAATCTGACTCTCTCTCCCTCCGACGACTTGTTCTTCAAAGGGAAGCTTGTGCCCATCGAGCCGTCGTTGATTGAGTTCAACCCATCTGAAGAATCCAACTCCAAGCCCCAATTCGCCGGCTCATTGTTGAAATCGGCCACCAAGTTTCGTGTTTTCATGCTGGGTTTGAAGAAATCGAAGTCGAATTCAAACGCGTCGGAAAAACCAGAGAAAACGGAGCCGCCGGCCGTGGGGTCCGTTGCGGCGGTTTCGAAAGAAGCGCCGCAAAAAACCCAGGAAAAACCACCGGAAGAGGAACCGCCGCAAAAGCAGAAACAGCAGGGGAAGCTTTTCACGGTGAAATTCAAGGTGGAGGAGGTCCCCATTGTGTCTTTGTTTACAAGAGACAATAGCTCGAGAATCTCTACAGCTAAAAAGTCTCAGACGACGACCCAGGAGAAGCCGACGCCGATAAACGCTTCTGAAGAATCGGCTTCTGAGGAAAAGCGCTTTTCCAAAGAGGTAATGCAAAAGTACTTGAAAATGGTTAAGCCGCTCTACGTTCGCGTTTCAAAGAGGTACGGCGAGAAGCTTAGACTGTCTGGGCAGCTCAGCTTGAACGGCTCAACAGCGGCGCCGGCTGAGGCTGAGAAGACCCAACCGGGAGCAGAAGTGTCGGAGCCCCCGCCGGCGACTGCGAGCAATGCGAAGAGTGGTCAGAAACAGGGGAATATCCCGGCTGGGCTGAGAGTGGTCTGCAAGCACTTGGGGAAGAGTCGGTCGGCTTCTTCCGCCGTAGCCGCGGCTCCATCGGGCGCCGTGGTCACTCAGAGGAGGGATGATTCGCTGCTTCAGCAGCAGGACGGAATCCAAAGCGCCATCCTGCATTGCAAGCGTTCCTTCAATGCCTCCCGAGCGATCCCTCGAACGAAAAAAAGTCAGTTGAGATGTCACGCAAGTCGTCGGATCAAGGCGCAGGAGGTGTGCTTTAACGGCCGAGATCTTTTTCCGCATGCTTTTTTGGCGGGAAATGGGGGGGAGTGGTCAAGTCAATGTTGCTGTTTGTTAATCATGCTCAAATCAAAGAAGGCCAAAAGGGGCAGTGGCCAGGTTTTCTTGTTAATTATTGTAAATTGCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

510

Amino Acids

55.23

Weight (kDa)

5.51

Isoelectric Point (pI)

48.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64080
fragaria_vesca FvH4_5g03470
malus_domestica MD06G1094800.v1.1 MD14G1115100.v1.1
prunus_persica Prupe.5G110000_v2.0.a1
pyrus_communis pycom06g09150
rosa_chinensis RchiOBHm_Chr7g0196191
rosa_laevigata RLG00000004053
rosa_multiflora Rmu_sc0007292.1_g000008
rosa_roxburghii Rroxscaffold_3G00259680
rosa_rugosa Rorug07G0032000
rosa_samantha Rh7AG157400 Rh7BG159100 Rh7CG163100 Rh7DG158400
rosa_wichuraiana Rw7G013460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 101
AccB1I GGYRCC 3 cut(s) 53, 1021, 1202
AccBSI CCGCTC 1 cut(s) 943
AccII CGCG 3 cut(s) 602, 954, 1190
AclWI GGATC 3 cut(s) 127, 1300, 1361
AcoI YGGCCR 5 cut(s) 231, 543, 633, 1381, 1492
AcsI RAATTY 2 cut(s) 592, 749
AcuI CTGAAG 3 cut(s) 516, 891, 1223
AcyI GRCGYC 3 cut(s) 854, 1022, 1203
AfaI GTAC 5 cut(s) 26, 274, 397, 923, 968
AfeI AGCGCT 1 cut(s) 896
AfiI CCNNNNNNNGG 1 cut(s) 638
AflIII ACRYGT 1 cut(s) 600
AjnI CCWGG 4 cut(s) 387, 681, 840, 1494
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
AluBI AGCT 9 cut(s) 8, 15, 454, 736, 801, 818, 979, 997, 1002
AluI AGCT 9 cut(s) 8, 15, 454, 736, 801, 818, 979, 997, 1002
Alw26I GTCTC 4 cut(s) 255, 376, 786, 831
AlwI GGATC 3 cut(s) 127, 1300, 1361
Ama87I CYCGRG 2 cut(s) 802, 1299
Aor51HI AGCGCT 1 cut(s) 896
AoxI GGCC 8 cut(s) 184, 231, 294, 543, 633, 1381, 1476, 1492
ApeKI GCWGC 3 cut(s) 994, 1234, 1243
ApoI RAATTY 2 cut(s) 592, 749
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
Asp700I GAANNNNTTC 1 cut(s) 737
AspLEI GCGC 7 cut(s) 102, 670, 897, 1024, 1205, 1265, 1363
AspS9I GGNCC 5 cut(s) 185, 295, 391, 642, 766
AsuC2I CCSGG 3 cut(s) 379, 1050, 1124
AsuHPI GGTGA 3 cut(s) 111, 130, 757
AsuII TTCGAA 1 cut(s) 659
AvaI CYCGRG 2 cut(s) 802, 1299
AvaII GGWCC 3 cut(s) 391, 642, 766
BaeGI GKGCMC 1 cut(s) 462
BalI TGGCCA 1 cut(s) 1494
BanI GGYRCC 3 cut(s) 53, 1021, 1202
BanII GRGCYC 1 cut(s) 1071
BbsI GAAGAC 3 cut(s) 171, 312, 1046
BbvCI CCTCAGC 1 cut(s) 1029
BbvI GCAGC 3 cut(s) 1006, 1221, 1255
BccI CCATC 5 cut(s) 338, 470, 499, 1204, 1274
BcgI CGANNNNNNTGC 2 cut(s) 1044, 1078
BciT130I CCWGG 4 cut(s) 389, 683, 842, 1496
BcnI CCSGG 3 cut(s) 379, 1050, 1124
BcoDI GTCTC 4 cut(s) 255, 376, 786, 831
BfaI CTAG 1 cut(s) 302
BfmI CTRYAG 1 cut(s) 813
BfoI RGCGCY 5 cut(s) 671, 898, 1025, 1206, 1266
BglII AGATCT 1 cut(s) 1387
BlpI GCTNAGC 1 cut(s) 998
BmcAI AGTACT 2 cut(s) 26, 923
Bme1390I CCNGG 7 cut(s) 379, 389, 683, 842, 1050, 1124, 1496
Bme18I GGWCC 3 cut(s) 391, 642, 766
BmeT110I CYCGRG 2 cut(s) 802, 1299
BmgT120I GGNCC 5 cut(s) 185, 295, 391, 642, 766
BmiI GGNNCC 9 cut(s) 55, 627, 643, 705, 768, 1023, 1068, 1194, 1204
BmrFI CCNGG 7 cut(s) 379, 389, 683, 842, 1050, 1124, 1496
BpiI GAAGAC 3 cut(s) 171, 312, 1046
Bpu10I CCTNAGC 1 cut(s) 1029
Bpu1102I GCTNAGC 1 cut(s) 998
Bpu14I TTCGAA 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 5
BpuMI CCSGG 3 cut(s) 379, 1050, 1124
BsaHI GRCGYC 3 cut(s) 854, 1022, 1203
BsaJI CCNNGG 5 cut(s) 636, 681, 840, 1188, 1206
BsaWI WCCGGW 1 cut(s) 694
BsaXI ACNNNNNCTCC 4 cut(s) 406, 436, 1044, 1074
Bsc4I CCNNNNNNNGG 1 cut(s) 638
Bse118I RCCGGY 4 cut(s) 524, 631, 1024, 1075
Bse1I ACTGG 1 cut(s) 32
Bse3DI GCAATG 2 cut(s) 1096, 1273
BseBI CCWGG 4 cut(s) 389, 683, 842, 1496
BseDI CCNNGG 5 cut(s) 636, 681, 840, 1188, 1206
BseGI GGATG 2 cut(s) 1231, 1266
BseLI CCNNNNNNNGG 1 cut(s) 638
BseMI GCAATG 2 cut(s) 1096, 1273
BseMII CTCAG 7 cut(s) 842, 876, 1012, 1020, 1026, 1124, 1229
BseNI ACTGG 1 cut(s) 32
BseRI GAGGAG 3 cut(s) 305, 776, 1234
BseSI GKGCMC 1 cut(s) 462
BseX3I CGGCCG 3 cut(s) 231, 633, 1381
BseXI GCAGC 3 cut(s) 1006, 1221, 1255
BseYI CCCAGC 2 cut(s) 568, 1127
Bsh1236I CGCG 3 cut(s) 602, 954, 1190
Bsh1285I CGRYCG 4 cut(s) 234, 636, 1169, 1384
BshFI GGCC 8 cut(s) 186, 233, 296, 545, 635, 1383, 1478, 1494
BshNI GGYRCC 3 cut(s) 53, 1021, 1202
BsiEI CGRYCG 4 cut(s) 234, 636, 1169, 1384
BsiHKCI CYCGRG 2 cut(s) 802, 1299
BsiSI CCGG 8 cut(s) 378, 525, 632, 695, 1025, 1049, 1076, 1124
BsiWI CGTACG 1 cut(s) 395
BslFI GGGAC 2 cut(s) 197, 752
BslI CCNNNNNNNGG 1 cut(s) 638
BsmAI GTCTC 4 cut(s) 255, 376, 786, 831
BsmBI CGTCTC 1 cut(s) 255
BsmFI GGGAC 2 cut(s) 197, 752
BsnI GGCC 8 cut(s) 186, 233, 296, 545, 635, 1383, 1478, 1494
BsoBI CYCGRG 2 cut(s) 802, 1299
Bsp119I TTCGAA 1 cut(s) 659
Bsp1286I GDGCHC 2 cut(s) 462, 1071
Bsp143I GATC 4 cut(s) 132, 1305, 1353, 1387
Bsp1720I GCTNAGC 1 cut(s) 998
BspANI GGCC 8 cut(s) 186, 233, 296, 545, 635, 1383, 1478, 1494
BspCNI CTCAG 7 cut(s) 841, 877, 1011, 1021, 1027, 1125, 1228
BspFNI CGCG 3 cut(s) 602, 954, 1190
BspLI GGNNCC 9 cut(s) 55, 627, 643, 705, 768, 1023, 1068, 1194, 1204
BspPI GGATC 3 cut(s) 127, 1300, 1361
BspT104I TTCGAA 1 cut(s) 659
BspT107I GGYRCC 3 cut(s) 53, 1021, 1202
BsrBI CCGCTC 1 cut(s) 943
BsrDI GCAATG 2 cut(s) 1096, 1273
BsrFI RCCGGY 4 cut(s) 524, 631, 1024, 1075
BsrI ACTGG 1 cut(s) 32
BssAI RCCGGY 4 cut(s) 524, 631, 1024, 1075
BssECI CCNNGG 5 cut(s) 636, 681, 840, 1188, 1206
BssMI GATC 4 cut(s) 132, 1305, 1353, 1387
BssNI GRCGYC 3 cut(s) 854, 1022, 1203
Bst2UI CCWGG 4 cut(s) 389, 683, 842, 1496
Bst4CI ACNGT 4 cut(s) 370, 395, 745, 987
Bst6I CTCTTC 4 cut(s) 379, 693, 1091, 1154
BstACI GRCGYC 3 cut(s) 854, 1022, 1203
BstBI TTCGAA 1 cut(s) 659
BstC8I GCNNGC 9 cut(s) 17, 526, 633, 1026, 1077, 1087, 1148, 1280, 1401
BstDEI CTNAG 8 cut(s) 828, 885, 980, 998, 1029, 1035, 1133, 1215
BstDSI CCRYGG 3 cut(s) 636, 1188, 1206
BstF5I GGATG 2 cut(s) 1231, 1266
BstFNI CGCG 3 cut(s) 602, 954, 1190
BstH2I RGCGCY 5 cut(s) 671, 898, 1025, 1206, 1266
BstHHI GCGC 7 cut(s) 102, 670, 897, 1024, 1205, 1265, 1363
BstKTI GATC 4 cut(s) 135, 1308, 1356, 1390
BstMAI GTCTC 4 cut(s) 255, 376, 786, 831
BstMBI GATC 4 cut(s) 132, 1305, 1353, 1387
BstMCI CGRYCG 4 cut(s) 234, 636, 1169, 1384
BstNI CCWGG 4 cut(s) 389, 683, 842, 1496
BstNSI RCATGY 1 cut(s) 1403
BstSCI CCNGG 7 cut(s) 377, 387, 681, 840, 1048, 1122, 1494
BstSFI CTRYAG 1 cut(s) 813
BstSLI GKGCMC 1 cut(s) 462
BstUI CGCG 3 cut(s) 602, 954, 1190
BstV1I GCAGC 3 cut(s) 1006, 1221, 1255
BstV2I GAAGAC 3 cut(s) 171, 312, 1046
BstX2I RGATCY 1 cut(s) 1387
BstXI CCANNNNNNTGG 1 cut(s) 257
BstYI RGATCY 1 cut(s) 1387
BstZI CGGCCG 3 cut(s) 231, 633, 1381
BsuRI GGCC 8 cut(s) 186, 233, 296, 545, 635, 1383, 1478, 1494
BtgI CCRYGG 3 cut(s) 636, 1188, 1206
BtgZI GCGATG 1 cut(s) 333
BtsCI GGATG 2 cut(s) 1231, 1266
BtsI GCAGTG 1 cut(s) 1495
BtsIMutI CAGTG 2 cut(s) 366, 1495
Cac8I GCNNGC 9 cut(s) 17, 526, 633, 1026, 1077, 1087, 1148, 1280, 1401
CciNI GCGGCCGC 1 cut(s) 231
CfoI GCGC 7 cut(s) 102, 670, 897, 1024, 1205, 1265, 1363
Cfr10I RCCGGY 4 cut(s) 524, 631, 1024, 1075
Cfr13I GGNCC 5 cut(s) 185, 295, 391, 642, 766
Cfr42I CCGCGG 1 cut(s) 1191
CseI GACGC 2 cut(s) 591, 862
Csp6I GTAC 5 cut(s) 25, 273, 396, 922, 967
CviAII CATG 3 cut(s) 565, 1400, 1459
CviQI GTAC 5 cut(s) 25, 273, 396, 922, 967
DdeI CTNAG 8 cut(s) 828, 885, 980, 998, 1029, 1035, 1133, 1215
DinI GGCGCC 2 cut(s) 1023, 1204
DpnI GATC 4 cut(s) 134, 1307, 1355, 1389
DpnII GATC 4 cut(s) 132, 1305, 1353, 1387
EaeI YGGCCR 5 cut(s) 231, 543, 633, 1381, 1492
EagI CGGCCG 3 cut(s) 231, 633, 1381
Eam1104I CTCTTC 4 cut(s) 379, 693, 1091, 1154
EarI CTCTTC 4 cut(s) 379, 693, 1091, 1154
EciI GGCGGA 1 cut(s) 1168
EclXI CGGCCG 3 cut(s) 231, 633, 1381
Eco24I GRGCYC 1 cut(s) 1071
Eco47I GGWCC 3 cut(s) 391, 642, 766
Eco47III AGCGCT 1 cut(s) 896
Eco52I CGGCCG 3 cut(s) 231, 633, 1381
Eco57I CTGAAG 3 cut(s) 516, 891, 1223
Eco88I CYCGRG 2 cut(s) 802, 1299
EcoO109I RGGNCCY 1 cut(s) 766
EcoRI GAATTC 1 cut(s) 592
EcoRII CCWGG 4 cut(s) 387, 681, 840, 1494
EcoT38I GRGCYC 1 cut(s) 1071
EgeI GGCGCC 2 cut(s) 1023, 1204
EheI GGCGCC 2 cut(s) 1023, 1204
Esp3I CGTCTC 1 cut(s) 255
FaeI CATG 3 cut(s) 568, 1403, 1462
FaiI YATR 4 cut(s) 51, 566, 1401, 1460
FalI AAGNNNNNCTT 4 cut(s) 438, 470, 1271, 1303
FaqI GGGAC 2 cut(s) 197, 752
FatI CATG 3 cut(s) 564, 1399, 1458
FauI CCCGC 2 cut(s) 1080, 1405
FokI GGATG 2 cut(s) 1238, 1253
FriOI GRGCYC 1 cut(s) 1071
FspAI RTGCGCAY 1 cut(s) 101
FspBI CTAG 1 cut(s) 302
FspI TGCGCA 1 cut(s) 101
GlaI GCGC 7 cut(s) 101, 669, 896, 1023, 1204, 1264, 1362
GsaI CCCAGC 2 cut(s) 572, 1131
HaeII RGCGCY 5 cut(s) 671, 898, 1025, 1206, 1266
HaeIII GGCC 8 cut(s) 186, 233, 296, 545, 635, 1383, 1478, 1494
HapII CCGG 8 cut(s) 378, 525, 632, 695, 1025, 1049, 1076, 1124
HgaI GACGC 2 cut(s) 591, 862
HhaI GCGC 7 cut(s) 102, 670, 897, 1024, 1205, 1265, 1363
Hin1I GRCGYC 3 cut(s) 854, 1022, 1203
Hin1II CATG 3 cut(s) 568, 1403, 1462
Hin6I GCGC 7 cut(s) 100, 668, 895, 1022, 1203, 1263, 1361
HinP1I GCGC 7 cut(s) 100, 668, 895, 1022, 1203, 1263, 1361
HindIII AAGCTT 4 cut(s) 6, 452, 734, 977
HpaII CCGG 8 cut(s) 378, 525, 632, 695, 1025, 1049, 1076, 1124
HphI GGTGA 3 cut(s) 111, 130, 757
Hpy166II GTNNAC 1 cut(s) 786
Hpy188III TCNNGA 3 cut(s) 83, 804, 1299
Hpy8I GTNNAC 1 cut(s) 786
HpyAV CCTTC 3 cut(s) 71, 1297, 1468
HpyCH4III ACNGT 4 cut(s) 370, 395, 745, 987
HpyCH4IV ACGT 1 cut(s) 948
HpyCH4V TGCA 4 cut(s) 916, 1146, 1273, 1278
HpyF3I CTNAG 8 cut(s) 828, 885, 980, 998, 1029, 1035, 1133, 1215
HpySE526I ACGT 1 cut(s) 948
Hsp92I GRCGYC 3 cut(s) 854, 1022, 1203
Hsp92II CATG 3 cut(s) 568, 1403, 1462
HspAI GCGC 7 cut(s) 100, 668, 895, 1022, 1203, 1263, 1361
KasI GGCGCC 2 cut(s) 1021, 1202
KroI GCCGGC 4 cut(s) 524, 631, 1024, 1075
KroNI GCCGGC 4 cut(s) 526, 633, 1026, 1077
KspI CCGCGG 1 cut(s) 1191
Kzo9I GATC 4 cut(s) 132, 1305, 1353, 1387
LmnI GCTCC 4 cut(s) 625, 1052, 1066, 1198
Lsp1109I GCAGC 3 cut(s) 1006, 1221, 1255
MaeI CTAG 1 cut(s) 302
MaeII ACGT 1 cut(s) 948
MaeIII GTNAC 2 cut(s) 1210, 1337
MalI GATC 4 cut(s) 134, 1307, 1355, 1389
MbiI CCGCTC 1 cut(s) 943
MboI GATC 4 cut(s) 132, 1305, 1353, 1387
MflI RGATCY 1 cut(s) 1387
MhlI GDGCHC 2 cut(s) 462, 1071
MlsI TGGCCA 1 cut(s) 1494
MluCI AATT 6 cut(s) 67, 518, 592, 749, 1509, 1519
MluI ACGCGT 1 cut(s) 600
MluNI TGGCCA 1 cut(s) 1494
Mly113I GGCGCC 2 cut(s) 1022, 1203
MlyI GAGTC 3 cut(s) 335, 409, 1171
MmeI TCCRAC 5 cut(s) 453, 528, 585, 1044, 1331
Mox20I TGGCCA 1 cut(s) 1494
MreI CGCCGGCG 1 cut(s) 1075
MroNI GCCGGC 4 cut(s) 524, 631, 1024, 1075
MroXI GAANNNNTTC 1 cut(s) 737
MscI TGGCCA 1 cut(s) 1494
MseI TTAA 4 cut(s) 936, 1377, 1454, 1508
Msp20I TGGCCA 1 cut(s) 1494
MspA1I CMGCKG 2 cut(s) 1019, 1190
MspI CCGG 8 cut(s) 378, 525, 632, 695, 1025, 1049, 1076, 1124
MspR9I CCNGG 7 cut(s) 379, 389, 683, 842, 1050, 1124, 1496
MvaI CCWGG 4 cut(s) 389, 683, 842, 1496
MvnI CGCG 3 cut(s) 602, 954, 1190
NaeI GCCGGC 4 cut(s) 526, 633, 1026, 1077
NarI GGCGCC 2 cut(s) 1022, 1203
NciI CCSGG 3 cut(s) 379, 1050, 1124
NdeII GATC 4 cut(s) 132, 1305, 1353, 1387
NgoMIV GCCGGC 4 cut(s) 524, 631, 1024, 1075
NlaIII CATG 3 cut(s) 568, 1403, 1462
NlaIV GGNNCC 9 cut(s) 55, 627, 643, 705, 768, 1023, 1068, 1194, 1204
NmeAIII GCCGAG 1 cut(s) 1409
NmuCI GTSAC 2 cut(s) 1210, 1337
NotI GCGGCCGC 1 cut(s) 231
NsbI TGCGCA 1 cut(s) 101
NspI RCATGY 1 cut(s) 1403
NspV TTCGAA 1 cut(s) 659
PaeI GCATGC 1 cut(s) 1403
PaeR7I CTCGAG 1 cut(s) 802
PcsI WCGNNNNNNNCGW 6 cut(s) 168, 285, 315, 321, 330, 404
PdiI GCCGGC 4 cut(s) 526, 633, 1026, 1077
PdmI GAANNNNTTC 1 cut(s) 737
PfeI GAWTC 7 cut(s) 86, 409, 500, 807, 875, 1229, 1254
Pfl23II CGTACG 1 cut(s) 395
PleI GAGTC 3 cut(s) 334, 409, 1170
PluTI GGCGCC 2 cut(s) 1025, 1206
PpsI GAGTC 3 cut(s) 334, 409, 1170
PpuMI RGGWCCY 1 cut(s) 766
Psp5II RGGWCCY 1 cut(s) 766
Psp6I CCWGG 4 cut(s) 387, 681, 840, 1494
PspFI CCCAGC 2 cut(s) 568, 1127
PspGI CCWGG 4 cut(s) 387, 681, 840, 1494
PspLI CGTACG 1 cut(s) 395
PspN4I GGNNCC 9 cut(s) 55, 627, 643, 705, 768, 1023, 1068, 1194, 1204
PspPI GGNCC 5 cut(s) 185, 295, 391, 642, 766
PspPPI RGGWCCY 1 cut(s) 766
PsuI RGATCY 1 cut(s) 1387
RsaI GTAC 5 cut(s) 26, 274, 397, 923, 968
RsaNI GTAC 5 cut(s) 25, 273, 396, 922, 967
SacII CCGCGG 1 cut(s) 1191
SaqAI TTAA 4 cut(s) 936, 1377, 1454, 1508
Sau3AI GATC 4 cut(s) 132, 1305, 1353, 1387
Sau96I GGNCC 5 cut(s) 185, 295, 391, 642, 766
ScaI AGTACT 2 cut(s) 26, 923
SchI GAGTC 3 cut(s) 335, 409, 1171
ScrFI CCNGG 7 cut(s) 379, 389, 683, 842, 1050, 1124, 1496
SduI GDGCHC 2 cut(s) 462, 1071
SfcI CTRYAG 1 cut(s) 813
SfoI GGCGCC 2 cut(s) 1023, 1204
Sfr274I CTCGAG 1 cut(s) 802
Sfr303I CCGCGG 1 cut(s) 1191
SfuI TTCGAA 1 cut(s) 659
SgrAI CRCCGGYG 1 cut(s) 1075
SgrBI CCGCGG 1 cut(s) 1191
SinI GGWCC 3 cut(s) 391, 642, 766
SlaI CTCGAG 1 cut(s) 802
SmlI CTYRAG 2 cut(s) 20, 802
SmoI CTYRAG 2 cut(s) 20, 802
SphI GCATGC 1 cut(s) 1403
Sse9I AATT 6 cut(s) 67, 518, 592, 749, 1509, 1519
SspDI GGCGCC 2 cut(s) 1021, 1202
SspMI CTAG 1 cut(s) 302
StyD4I CCNGG 7 cut(s) 377, 387, 681, 840, 1048, 1122, 1494
TaaI ACNGT 4 cut(s) 370, 395, 745, 987
TaiI ACGT 1 cut(s) 951
TaqI TCGA 8 cut(s) 195, 383, 465, 584, 590, 659, 803, 1312
TaqII GACCGA 1 cut(s) 1155
TasI AATT 6 cut(s) 67, 518, 592, 749, 1509, 1519
TatI WGTACW 2 cut(s) 24, 921
TfiI GAWTC 7 cut(s) 86, 409, 500, 807, 875, 1229, 1254
Tru1I TTAA 4 cut(s) 936, 1377, 1454, 1508
Tru9I TTAA 4 cut(s) 936, 1377, 1454, 1508
TscAI CASTG 2 cut(s) 373, 1495
TseFI GTSAC 2 cut(s) 1210, 1337
TseI GCWGC 3 cut(s) 994, 1234, 1243
Tsp45I GTSAC 2 cut(s) 1210, 1337
TspDTI ATGAA 2 cut(s) 240, 553
TspGWI ACGGA 6 cut(s) 279, 290, 413, 634, 638, 1266
TspRI CASTG 2 cut(s) 373, 1495
VpaK11BI GGWCC 3 cut(s) 391, 642, 766
XapI RAATTY 2 cut(s) 592, 749
XceI RCATGY 1 cut(s) 1403
XcmI CCANNNNNNNNNTGG 1 cut(s) 254
XhoI CTCGAG 1 cut(s) 802
XmnI GAANNNNTTC 1 cut(s) 737
XspI CTAG 1 cut(s) 302
ZrmI AGTACT 2 cut(s) 26, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.