AT1G64970
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. gTMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011326
Physical Location & Seq
Reverse (-)
24134085 .. 24136109
2025 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G64970.1

Sequence Viewer

Length: 1047 bp
ATGAAAGCAACTCTAGCAGCACCCTCTTCTCTCACAAGCCTCCCTTATCGAACCAACTCTTCTTTCGGCTCAAAGTCATCGCTTCTCTTTCGGTCTCCATCCTCCTCCTCCTCAGTCTCTATGACGACAACGCGTGGAAACGTGGCTGTGGCGGCTGCTGCTACATCCACTGAGGCGCTAAGAAAAGGAATAGCGGAGTTCTACAATGAAACTTCGGGTTTGTGGGAAGAGATTTGGGGAGATCATATGCATCATGGCTTTTATGACCCTGATTCTTCTGTTCAACTTTCTGATTCTGGTCACAAGGAAGCTCAGATCCGTATGATTGAAGAGTCTCTCCGTTTTGCCGGTGTTACTGATGAAGAGGAGGAGAAAAAGATAAAGAAAGTAGTGGATGTTGGGTGTGGGATTGGAGGAAGCTCAAGATATCTTGCCTCTAAATTTGGAGCTGAATGCATTGGCATTACTCTCAGCCCTGTTCAGGCCAAGAGAGCCAATGATCTCGCGGCTGCTCAATCACTCGCTCATAAGGCTTCCTTCCAAGTTGCGGATGCGTTGGATCAGCCATTCGAAGATGGAAAATTCGATCTAGTGTGGTCGATGGAGAGTGGTGAGCATATGCCTGACAAGGCCAAGTTTGTAAAAGAGTTGGTACGTGTGGCGGCTCCAGGAGGTAGGATAATAATAGTGACATGGTGCCATAGAAATCTATCTGCGGGGGAGGAAGCTTTGCAGCCGTGGGAGCAAAACATCTTGGACAAAATCTGTAAGACGTTCTATCTCCCGGCTTGGTGCTCCACCGATGATTATGTCAACTTGCTTCAATCCCATTCTCTCCAGGATATTAAGTGTGCGGATTGGTCAGAGAACGTAGCTCCTTTCTGGCCTGCGGTTATACGGACTGCATTAACATGGAAGGGCCTTGTGTCTCTGCTTCGTAGTGGTATGAAAAGTATTAAAGGAGCATTGACAATGCCATTGATGATTGAAGGTTACAAGAAAGGTGTCATTAAGTTTGGTATCATCACTTGCCAGAAGCCACTCTAA

Protein Analysis

348

Amino Acids

38.08

Weight (kDa)

6.72

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 120 - 259 5.5e-09 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 122 - 247 4.8e-14 Methyltransferase domain
Methyltransf_31 PF13847 126 - 243 3.8e-17 Methyltransferase domain
CMAS PF02353 128 - 234 1.2e-12 Mycolic acid cyclopropane synthetase
Methyltransf_25 PF13649 130 - 225 3.5e-20 Methyltransferase domain
Methyltransf_11 PF08241 131 - 229 1.4e-22 Methyltransferase domain
Methyltransf_12 PF08242 131 - 227 1e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 696
AccII CGCG 2 cut(s) 133, 506
AciI CCGC 8 cut(s) 152, 194, 506, 548, 662, 716, 854, 890
AclWI GGATC 2 cut(s) 310, 567
AcsI RAATTY 2 cut(s) 440, 581
AfaI GTAC 1 cut(s) 654
AfiI CCNNNNNNNGG 2 cut(s) 481, 547
AflIII ACRYGT 2 cut(s) 131, 655
AgsI TTSAA 4 cut(s) 284, 329, 824, 989
AjnI CCWGG 2 cut(s) 667, 837
AjuI GAANNNNNNNTTGG 2 cut(s) 47, 79
AluBI AGCT 5 cut(s) 311, 420, 449, 728, 875
AluI AGCT 5 cut(s) 311, 420, 449, 728, 875
Alw21I GWGCWC 1 cut(s) 797
Alw26I GTCTC 4 cut(s) 99, 121, 339, 933
AlwI GGATC 2 cut(s) 310, 567
AoxI GGCC 4 cut(s) 483, 630, 884, 919
ApeKI GCWGC 5 cut(s) 17, 155, 158, 509, 733
ApoI RAATTY 2 cut(s) 440, 581
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 1 cut(s) 919
AsuC2I CCSGG 1 cut(s) 785
AsuHPI GGTGA 1 cut(s) 623
AsuII TTCGAA 1 cut(s) 570
BanI GGYRCC 1 cut(s) 696
Bbv12I GWGCWC 1 cut(s) 797
BbvI GCAGC 5 cut(s) 29, 142, 145, 496, 745
BccI CCATC 3 cut(s) 106, 569, 595
BceAI ACGGC 1 cut(s) 721
BciT130I CCWGG 2 cut(s) 669, 839
BcnI CCSGG 1 cut(s) 785
BcoDI GTCTC 4 cut(s) 99, 121, 339, 933
BfaI CTAG 2 cut(s) 14, 590
BfoI RGCGCY 1 cut(s) 179
BisI GCNGC 8 cut(s) 18, 153, 156, 159, 507, 510, 663, 734
BlsI GCNGC 8 cut(s) 19, 154, 157, 160, 508, 511, 664, 735
Bme1390I CCNGG 3 cut(s) 669, 785, 839
BmgT120I GGNCC 1 cut(s) 919
BmiI GGNNCC 2 cut(s) 666, 698
BmrFI CCNGG 3 cut(s) 669, 785, 839
BmsI GCATC 2 cut(s) 259, 541
BpmI CTGGAG 2 cut(s) 651, 821
Bpu14I TTCGAA 1 cut(s) 570
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 785
BsaAI YACGTR 1 cut(s) 656
BsaI GGTCTC 1 cut(s) 99
BsaJI CCNNGG 1 cut(s) 737
Bsc4I CCNNNNNNNGG 2 cut(s) 481, 547
Bse118I RCCGGY 1 cut(s) 347
BseBI CCWGG 2 cut(s) 669, 839
BseDI CCNNGG 1 cut(s) 737
BseGI GGATG 4 cut(s) 98, 164, 400, 556
BseLI CCNNNNNNNGG 2 cut(s) 481, 547
BseMII CTCAG 4 cut(s) 126, 162, 326, 484
BseRI GAGGAG 5 cut(s) 94, 97, 100, 380, 383
BseXI GCAGC 5 cut(s) 29, 142, 145, 496, 745
Bsh1236I CGCG 2 cut(s) 133, 506
BshFI GGCC 4 cut(s) 485, 632, 886, 921
BshNI GGYRCC 1 cut(s) 696
BsiHKAI GWGCWC 1 cut(s) 797
BsiSI CCGG 2 cut(s) 348, 785
BslI CCNNNNNNNGG 2 cut(s) 481, 547
BsmAI GTCTC 4 cut(s) 99, 121, 339, 933
BsmI GAATGC 1 cut(s) 458
BsnI GGCC 4 cut(s) 485, 632, 886, 921
Bso31I GGTCTC 1 cut(s) 99
Bsp119I TTCGAA 1 cut(s) 570
Bsp1286I GDGCHC 1 cut(s) 797
Bsp143I GATC 5 cut(s) 241, 315, 499, 559, 586
BspACI CCGC 8 cut(s) 152, 194, 506, 548, 662, 716, 854, 890
BspANI GGCC 4 cut(s) 485, 632, 886, 921
BspCNI CTCAG 4 cut(s) 125, 163, 325, 483
BspFNI CGCG 2 cut(s) 133, 506
BspLI GGNNCC 2 cut(s) 666, 698
BspPI GGATC 2 cut(s) 310, 567
BspT104I TTCGAA 1 cut(s) 570
BspT107I GGYRCC 1 cut(s) 696
BspTNI GGTCTC 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 347
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 1 cut(s) 737
BssMI GATC 5 cut(s) 241, 315, 499, 559, 586
Bst2UI CCWGG 2 cut(s) 669, 839
Bst6I CTCTTC 5 cut(s) 31, 64, 222, 324, 357
BstBAI YACGTR 1 cut(s) 656
BstBI TTCGAA 1 cut(s) 570
BstC8I GCNNGC 1 cut(s) 888
BstDEI CTNAG 5 cut(s) 112, 171, 179, 312, 470
BstDSI CCRYGG 1 cut(s) 737
BstF5I GGATG 4 cut(s) 98, 164, 400, 556
BstFNI CGCG 2 cut(s) 133, 506
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 5 cut(s) 244, 318, 502, 562, 589
BstMAI GTCTC 4 cut(s) 99, 121, 339, 933
BstMBI GATC 5 cut(s) 241, 315, 499, 559, 586
BstMWI GCNNNNNNNGC 6 cut(s) 14, 152, 158, 491, 530, 742
BstNI CCWGG 2 cut(s) 669, 839
BstSCI CCNGG 3 cut(s) 667, 783, 837
BstUI CGCG 2 cut(s) 133, 506
BstV1I GCAGC 5 cut(s) 29, 142, 145, 496, 745
BstX2I RGATCY 1 cut(s) 315
BstYI RGATCY 1 cut(s) 315
BsuRI GGCC 4 cut(s) 485, 632, 886, 921
BtgI CCRYGG 1 cut(s) 737
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 4 cut(s) 98, 164, 400, 556
BtsIMutI CAGTG 1 cut(s) 168
Cac8I GCNNGC 1 cut(s) 888
CfoI GCGC 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 1 cut(s) 919
Csp6I GTAC 1 cut(s) 653
CviAII CATG 3 cut(s) 254, 693, 912
CviQI GTAC 1 cut(s) 653
DdeI CTNAG 5 cut(s) 112, 171, 179, 312, 470
DpnI GATC 5 cut(s) 243, 317, 501, 561, 588
DpnII GATC 5 cut(s) 241, 315, 499, 559, 586
Eam1104I CTCTTC 5 cut(s) 31, 64, 222, 324, 357
EarI CTCTTC 5 cut(s) 31, 64, 222, 324, 357
Eco31I GGTCTC 1 cut(s) 99
Eco32I GATATC 1 cut(s) 428
EcoO109I RGGNCCY 1 cut(s) 919
EcoRII CCWGG 2 cut(s) 667, 837
EcoRV GATATC 1 cut(s) 428
EcoT22I ATGCAT 2 cut(s) 252, 458
FaeI CATG 3 cut(s) 257, 696, 915
FalI AAGNNNNNCTT 4 cut(s) 28, 60, 521, 553
FatI CATG 3 cut(s) 253, 692, 911
FauI CCCGC 1 cut(s) 709
FauNDI CATATG 2 cut(s) 246, 618
Fnu4HI GCNGC 8 cut(s) 18, 153, 156, 159, 507, 510, 663, 734
FokI GGATG 4 cut(s) 85, 151, 407, 563
Fsp4HI GCNGC 8 cut(s) 18, 153, 156, 159, 507, 510, 663, 734
FspBI CTAG 2 cut(s) 14, 590
GlaI GCGC 1 cut(s) 177
GluI GCNGC 8 cut(s) 18, 153, 156, 159, 507, 510, 663, 734
GsuI CTGGAG 2 cut(s) 651, 821
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 4 cut(s) 485, 632, 886, 921
HapII CCGG 2 cut(s) 348, 785
HhaI GCGC 1 cut(s) 178
Hin1II CATG 3 cut(s) 257, 696, 915
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HindIII AAGCTT 1 cut(s) 726
HinfI GANTC 3 cut(s) 272, 293, 332
HpaII CCGG 2 cut(s) 348, 785
HphI GGTGA 1 cut(s) 623
Hpy166II GTNNAC 1 cut(s) 814
Hpy188I TCNGA 3 cut(s) 292, 315, 865
Hpy188III TCNNGA 1 cut(s) 423
Hpy8I GTNNAC 1 cut(s) 814
HpyAV CCTTC 3 cut(s) 547, 910, 983
HpyCH4IV ACGT 4 cut(s) 141, 655, 773, 870
HpyCH4V TGCA 4 cut(s) 250, 456, 733, 905
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 152, 158, 491, 530, 742
HpyF3I CTNAG 5 cut(s) 112, 171, 179, 312, 470
HpySE526I ACGT 4 cut(s) 141, 655, 773, 870
Hsp92II CATG 3 cut(s) 257, 696, 915
HspAI GCGC 1 cut(s) 176
Kzo9I GATC 5 cut(s) 241, 315, 499, 559, 586
LmnI GCTCC 6 cut(s) 446, 670, 742, 800, 880, 962
Lsp1109I GCAGC 5 cut(s) 29, 142, 145, 496, 745
LweI GCATC 2 cut(s) 259, 541
MaeI CTAG 2 cut(s) 14, 590
MaeII ACGT 4 cut(s) 141, 655, 773, 870
MaeIII GTNAC 4 cut(s) 299, 352, 688, 992
MalI GATC 5 cut(s) 243, 317, 501, 561, 588
MboI GATC 5 cut(s) 241, 315, 499, 559, 586
MboII GAAGA 7 cut(s) 18, 51, 239, 267, 341, 374, 584
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 1 cut(s) 797
MluCI AATT 2 cut(s) 440, 581
MluI ACGCGT 1 cut(s) 131
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 537
Mph1103I ATGCAT 2 cut(s) 252, 458
MseI TTAA 4 cut(s) 846, 908, 957, 1011
MslI CAYNNNNRTG 1 cut(s) 910
MspI CCGG 2 cut(s) 348, 785
MspR9I CCNGG 3 cut(s) 669, 785, 839
Mva1269I GAATGC 1 cut(s) 458
MvaI CCWGG 2 cut(s) 669, 839
MvnI CGCG 2 cut(s) 133, 506
MwoI GCNNNNNNNGC 6 cut(s) 14, 152, 158, 491, 530, 742
NciI CCSGG 1 cut(s) 785
NdeI CATATG 2 cut(s) 246, 618
NdeII GATC 5 cut(s) 241, 315, 499, 559, 586
NlaIII CATG 3 cut(s) 257, 696, 915
NlaIV GGNNCC 2 cut(s) 666, 698
NmuCI GTSAC 2 cut(s) 299, 688
NsiI ATGCAT 2 cut(s) 252, 458
NspV TTCGAA 1 cut(s) 570
PctI GAATGC 1 cut(s) 458
PfeI GAWTC 2 cut(s) 272, 293
PfoI TCCNGGA 2 cut(s) 667, 837
PkrI GCNGC 8 cut(s) 19, 154, 157, 160, 508, 511, 664, 735
PleI GAGTC 1 cut(s) 340
PpsI GAGTC 1 cut(s) 340
Ppu21I YACGTR 1 cut(s) 656
Psp6I CCWGG 2 cut(s) 667, 837
PspGI CCWGG 2 cut(s) 667, 837
PspN4I GGNNCC 2 cut(s) 666, 698
PspPI GGNCC 1 cut(s) 919
PsuI RGATCY 1 cut(s) 315
RsaI GTAC 1 cut(s) 654
RsaNI GTAC 1 cut(s) 653
RseI CAYNNNNRTG 1 cut(s) 910
SaqAI TTAA 4 cut(s) 846, 908, 957, 1011
SatI GCNGC 8 cut(s) 18, 153, 156, 159, 507, 510, 663, 734
Sau3AI GATC 5 cut(s) 241, 315, 499, 559, 586
Sau96I GGNCC 1 cut(s) 919
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 3 cut(s) 669, 785, 839
SduI GDGCHC 1 cut(s) 797
SfaNI GCATC 2 cut(s) 259, 541
SfuI TTCGAA 1 cut(s) 570
SmiMI CAYNNNNRTG 1 cut(s) 910
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
Sse9I AATT 2 cut(s) 440, 581
SsiI CCGC 8 cut(s) 152, 194, 506, 548, 662, 716, 854, 890
SspMI CTAG 2 cut(s) 14, 590
StyD4I CCNGG 3 cut(s) 667, 783, 837
TaiI ACGT 4 cut(s) 144, 658, 776, 873
TaqI TCGA 4 cut(s) 49, 570, 585, 599
TaqII GACCGA 1 cut(s) 81
TasI AATT 2 cut(s) 440, 581
TauI GCSGC 3 cut(s) 155, 509, 665
TfiI GAWTC 2 cut(s) 272, 293
Tru1I TTAA 4 cut(s) 846, 908, 957, 1011
Tru9I TTAA 4 cut(s) 846, 908, 957, 1011
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 2 cut(s) 299, 688
TseI GCWGC 5 cut(s) 17, 155, 158, 509, 733
Tsp45I GTSAC 2 cut(s) 299, 688
TspDTI ATGAA 4 cut(s) 17, 222, 375, 962
TspGWI ACGGA 3 cut(s) 308, 329, 913
TspRI CASTG 1 cut(s) 175
XapI RAATTY 2 cut(s) 440, 581
XspI CTAG 2 cut(s) 14, 590
Zsp2I ATGCAT 2 cut(s) 252, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.