Rh3AG173700
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. gTMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0011326
Physical Location & Seq
Reverse (-)
16091410 .. 16093625
2216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG173700.1

Sequence Viewer

Length: 1041 bp
ATGGCATCTACGAGGCTGAACTACCCGACGTGCCGGTGGTCCTCGCCAGCAGCAACTACGCTTCCTCCTCGCACGTGTCTGGGTGTGGCTGGTGTGAACGGAGCAGCGGTGAGCTCGAAGAAGAAGAAGAAGAGCGGGTTTGAGTTGAGAGCAGTGGCGGCGACGGCGATGGAGGCAGTGGAGCTGAAGAAGGACATAGCCGAGTTCTACGACGAGTCGTCTGGGTTATGGGAGGACCTCTGGGGCGACCACATGCACCACGGCTTCTACGACCCCAAGGCCGCCGACGTTTCGGTGTCGGACCACCGGGCGGCCCAGACCCGGATGATCGACGAGGTGCTACGGTTCGCAGGGATTTCTGACAAGTGTCCAATTGGGAATGTAGTGGATGTTGGGTGTGGGATTGGAGGGAGCTCCAGATATCTGGCTAGCAAATACGGGGCCAATTGCAAAGGGATTACTCTGAGCCCCGTACAGGCTCTGAGGGCCAATGCTCTTGCTGCTGCTCAAGGCCTAGCCAATAAGGCATCCTTTCAAGTTGCTGATGCTCTAGCACAACCATTTCCTGATGGCCAATTTGATCTGGTTTGGTCCATGGAGAGTGGGGAACACATGCCTGATAAAGCAAAGTTTGTTGATGAGTTGGTTCGAGTTGCGGCACCAGGAGCAACGATAATACTAGTTACATGGTGCCACAGGGATCTTGGCGCTTCTGAACAATCATTGCAGCCAGATGAGAAAAAGCTCCTGGACAAGATATGCAATGCTTTCTATCTTCCTGCTTGGTGTTCTACTGCTGACTACCTCAAGCTACTCGAGTCCTACTCACTCAAGGATATCAAGGCTGAGGACTGGTCTCCGTATGTTGCCCCATTTTGGCCGGCAGTGATTCGCTCAGCATTGACATTTAAGGGTTTCTTTTCTCTGTTGCGCACAGGAATGAAAACAATAAGAGGAGCATTGGCAATGCCATTGATGATCCAAGGATTCAAGAAGGATCTAATTAAGTATTCCATTATTACATGCCGAAAACCTGAATAG

Protein Analysis

346

Amino Acids

37.75

Weight (kDa)

8.46

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 64 - 227 5.3e-11 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 104 - 242 1.1e-12 Methyltransferase domain
Methyltransf_31 PF13847 127 - 238 2.5e-12 Methyltransferase domain
Methyltransf_25 PF13649 128 - 222 1.6e-17 Methyltransferase domain
Methyltransf_11 PF08241 129 - 226 1.8e-19 Methyltransferase domain
Methyltransf_12 PF08242 129 - 224 2.5e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 932
AccB1I GGYRCC 2 cut(s) 658, 690
AccBSI CCGCTC 1 cut(s) 135
AciI CCGC 6 cut(s) 107, 135, 158, 282, 311, 656
AclWI GGATC 3 cut(s) 708, 973, 1005
AcoI YGGCCR 2 cut(s) 571, 878
AcuI CTGAAG 1 cut(s) 206
AcvI CACGTG 1 cut(s) 75
AfaI GTAC 1 cut(s) 474
AfiI CCNNNNNNNGG 4 cut(s) 310, 321, 475, 876
AflIII ACRYGT 1 cut(s) 74
AgsI TTSAA 2 cut(s) 536, 991
AhdI GACNNNNNGTC 1 cut(s) 217
AhlI ACTAGT 1 cut(s) 679
AjiI CACGTC 1 cut(s) 30
AjnI CCWGG 2 cut(s) 661, 747
AluBI AGCT 5 cut(s) 114, 184, 414, 745, 811
AluI AGCT 5 cut(s) 114, 184, 414, 745, 811
Alw21I GWGCWC 2 cut(s) 116, 416
Alw26I GTCTC 1 cut(s) 861
AlwI GGATC 3 cut(s) 708, 973, 1005
AlwNI CAGNNNCTG 1 cut(s) 481
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 7 cut(s) 279, 312, 441, 486, 511, 571, 878
ApeKI GCWGC 5 cut(s) 50, 104, 500, 503, 727
AspLEI GCGC 2 cut(s) 710, 933
AspS9I GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
AsuC2I CCSGG 2 cut(s) 308, 322
AsuHPI GGTGA 1 cut(s) 121
AsuNHI GCTAGC 1 cut(s) 428
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 4 cut(s) 39, 235, 301, 591
BalI TGGCCA 1 cut(s) 573
BanI GGYRCC 2 cut(s) 658, 690
BanII GRGCYC 3 cut(s) 116, 416, 470
BbrPI CACGTG 1 cut(s) 75
Bbv12I GWGCWC 2 cut(s) 116, 416
BbvCI CCTCAGC 1 cut(s) 846
BbvI GCAGC 5 cut(s) 62, 116, 487, 490, 739
BccI CCATC 2 cut(s) 163, 563
BceAI ACGGC 2 cut(s) 180, 277
BciT130I CCWGG 2 cut(s) 663, 749
BcnI CCSGG 2 cut(s) 308, 322
BcoDI GTCTC 1 cut(s) 861
BcuI ACTAGT 1 cut(s) 679
BfaI CTAG 4 cut(s) 429, 515, 551, 680
BfoI RGCGCY 1 cut(s) 711
BglI GCCNNNNNGGC 1 cut(s) 524
BisI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 501, 504, 657, 728
BlpI GCTNAGC 1 cut(s) 895
BlsI GCNGC 9 cut(s) 52, 106, 160, 283, 313, 502, 505, 658, 729
Bme1390I CCNGG 4 cut(s) 308, 322, 663, 749
Bme18I GGWCC 4 cut(s) 39, 235, 301, 591
BmeRI GACNNNNNGTC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 815
BmgBI CACGTC 1 cut(s) 30
BmgT120I GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
BmiI GGNNCC 3 cut(s) 442, 660, 692
BmrFI CCNGG 4 cut(s) 308, 322, 663, 749
BmsI GCATC 3 cut(s) 14, 535, 536
BmtI GCTAGC 1 cut(s) 432
BoxI GACNNNNGTC 1 cut(s) 366
BplI GAGNNNNNCTC 2 cut(s) 809, 841
BpmI CTGGAG 1 cut(s) 400
Bpu10I CCTNAGC 1 cut(s) 846
Bpu1102I GCTNAGC 1 cut(s) 895
BpuEI CTTGAG 3 cut(s) 492, 791, 815
BpuMI CCSGG 2 cut(s) 308, 322
BsaAI YACGTR 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 861
BsaJI CCNNGG 4 cut(s) 259, 276, 594, 982
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 93, 123
Bsc4I CCNNNNNNNGG 4 cut(s) 310, 321, 475, 876
Bse118I RCCGGY 2 cut(s) 33, 880
Bse1I ACTGG 1 cut(s) 857
Bse3DI GCAATG 3 cut(s) 722, 769, 972
BseBI CCWGG 2 cut(s) 663, 749
BseDI CCNNGG 4 cut(s) 259, 276, 594, 982
BseGI GGATG 3 cut(s) 330, 394, 527
BseLI CCNNNNNNNGG 4 cut(s) 310, 321, 475, 876
BseMI GCAATG 3 cut(s) 722, 769, 972
BseMII CTCAG 4 cut(s) 455, 473, 837, 909
BseNI ACTGG 1 cut(s) 857
BseRI GAGGAG 2 cut(s) 57, 969
BseXI GCAGC 5 cut(s) 62, 116, 487, 490, 739
BshFI GGCC 7 cut(s) 281, 314, 443, 488, 513, 573, 880
BshNI GGYRCC 2 cut(s) 658, 690
BsiHKAI GWGCWC 2 cut(s) 116, 416
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 4 cut(s) 34, 307, 322, 881
BslI CCNNNNNNNGG 4 cut(s) 310, 321, 475, 876
BsmAI GTCTC 1 cut(s) 861
BsnI GGCC 7 cut(s) 281, 314, 443, 488, 513, 573, 880
Bso31I GGTCTC 1 cut(s) 861
BsoBI CYCGRG 1 cut(s) 815
Bsp1286I GDGCHC 3 cut(s) 116, 416, 470
Bsp143I GATC 5 cut(s) 327, 580, 700, 978, 997
Bsp1720I GCTNAGC 1 cut(s) 895
Bsp19I CCATGG 1 cut(s) 594
BspACI CCGC 6 cut(s) 107, 135, 158, 282, 311, 656
BspANI GGCC 7 cut(s) 281, 314, 443, 488, 513, 573, 880
BspCNI CTCAG 4 cut(s) 456, 474, 838, 908
BspLI GGNNCC 3 cut(s) 442, 660, 692
BspOI GCTAGC 1 cut(s) 432
BspPI GGATC 3 cut(s) 708, 973, 1005
BspQI GCTCTTC 1 cut(s) 125
BspT107I GGYRCC 2 cut(s) 658, 690
BspTNI GGTCTC 1 cut(s) 861
BsrBI CCGCTC 1 cut(s) 135
BsrDI GCAATG 3 cut(s) 722, 769, 972
BsrFI RCCGGY 2 cut(s) 33, 880
BsrI ACTGG 1 cut(s) 857
BssAI RCCGGY 2 cut(s) 33, 880
BssECI CCNNGG 4 cut(s) 259, 276, 594, 982
BssMI GATC 5 cut(s) 327, 580, 700, 978, 997
BssT1I CCWWGG 3 cut(s) 276, 594, 982
Bst2UI CCWGG 2 cut(s) 663, 749
Bst4CI ACNGT 1 cut(s) 345
Bst6I CTCTTC 1 cut(s) 125
BstBAI YACGTR 1 cut(s) 75
BstC8I GCNNGC 3 cut(s) 48, 430, 882
BstDEI CTNAG 4 cut(s) 464, 482, 846, 895
BstDSI CCRYGG 2 cut(s) 259, 594
BstF5I GGATG 3 cut(s) 330, 394, 527
BstH2I RGCGCY 1 cut(s) 711
BstHHI GCGC 2 cut(s) 710, 933
BstKTI GATC 5 cut(s) 330, 583, 703, 981, 1000
BstMAI GTCTC 1 cut(s) 861
BstMBI GATC 5 cut(s) 327, 580, 700, 978, 997
BstMWI GCNNNNNNNGC 7 cut(s) 158, 164, 173, 485, 500, 524, 665
BstNI CCWGG 2 cut(s) 663, 749
BstNSI RCATGY 3 cut(s) 256, 616, 1026
BstPAI GACNNNNGTC 1 cut(s) 366
BstSCI CCNGG 4 cut(s) 306, 320, 661, 747
BstV1I GCAGC 5 cut(s) 62, 116, 487, 490, 739
BstX2I RGATCY 2 cut(s) 700, 997
BstXI CCANNNNNNTGG 1 cut(s) 424
BstYI RGATCY 2 cut(s) 700, 997
BsuRI GGCC 7 cut(s) 281, 314, 443, 488, 513, 573, 880
BtgI CCRYGG 2 cut(s) 259, 594
BtgZI GCGATG 1 cut(s) 182
BtrI CACGTC 1 cut(s) 30
BtsCI GGATG 3 cut(s) 330, 394, 527
BtsI GCAGTG 3 cut(s) 159, 183, 891
BtsIMutI CAGTG 3 cut(s) 159, 183, 891
Cac8I GCNNGC 3 cut(s) 48, 430, 882
CaiI CAGNNNCTG 1 cut(s) 481
CfoI GCGC 2 cut(s) 710, 933
Cfr10I RCCGGY 2 cut(s) 33, 880
Cfr13I GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
Csp6I GTAC 1 cut(s) 473
CviAII CATG 5 cut(s) 253, 595, 613, 687, 1023
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 4 cut(s) 464, 482, 846, 895
DpnI GATC 5 cut(s) 329, 582, 702, 980, 999
DpnII GATC 5 cut(s) 327, 580, 700, 978, 997
DriI GACNNNNNGTC 1 cut(s) 217
EaeI YGGCCR 2 cut(s) 571, 878
Eam1104I CTCTTC 1 cut(s) 125
Eam1105I GACNNNNNGTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 125
Ecl136II GAGCTC 2 cut(s) 114, 414
Eco130I CCWWGG 3 cut(s) 276, 594, 982
Eco147I AGGCCT 1 cut(s) 513
Eco24I GRGCYC 3 cut(s) 116, 416, 470
Eco31I GGTCTC 1 cut(s) 861
Eco32I GATATC 2 cut(s) 422, 838
Eco47I GGWCC 4 cut(s) 39, 235, 301, 591
Eco53kI GAGCTC 2 cut(s) 114, 414
Eco57I CTGAAG 1 cut(s) 206
Eco72I CACGTG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 815
EcoICRI GAGCTC 2 cut(s) 114, 414
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 2 cut(s) 661, 747
EcoRV GATATC 2 cut(s) 422, 838
EcoT14I CCWWGG 3 cut(s) 276, 594, 982
EcoT38I GRGCYC 3 cut(s) 116, 416, 470
ErhI CCWWGG 3 cut(s) 276, 594, 982
FaeI CATG 5 cut(s) 256, 598, 616, 690, 1026
FaiI YATR 9 cut(s) 197, 229, 254, 596, 614, 688, 760, 864, 1024
FalI AAGNNNNNCTT 4 cut(s) 515, 547, 902, 934
FatI CATG 5 cut(s) 252, 594, 612, 686, 1022
FauI CCCGC 1 cut(s) 128
Fnu4HI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 501, 504, 657, 728
FokI GGATG 3 cut(s) 337, 401, 514
FriOI GRGCYC 3 cut(s) 116, 416, 470
Fsp4HI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 501, 504, 657, 728
FspBI CTAG 4 cut(s) 429, 515, 551, 680
FspI TGCGCA 1 cut(s) 932
GlaI GCGC 2 cut(s) 709, 932
GluI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 501, 504, 657, 728
GsuI CTGGAG 1 cut(s) 400
HaeII RGCGCY 1 cut(s) 711
HaeIII GGCC 7 cut(s) 281, 314, 443, 488, 513, 573, 880
HapII CCGG 4 cut(s) 34, 307, 322, 881
HhaI GCGC 2 cut(s) 710, 933
Hin1II CATG 5 cut(s) 256, 598, 616, 690, 1026
Hin6I GCGC 2 cut(s) 708, 931
HinP1I GCGC 2 cut(s) 708, 931
HinfI GANTC 4 cut(s) 215, 818, 889, 987
HpaII CCGG 4 cut(s) 34, 307, 322, 881
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 5 cut(s) 301, 361, 465, 483, 715
Hpy188III TCNNGA 3 cut(s) 417, 566, 991
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 5 cut(s) 31, 166, 215, 290, 335
HpyAV CCTTC 2 cut(s) 184, 988
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4IV ACGT 3 cut(s) 29, 74, 288
HpyCH4V TGCA 4 cut(s) 256, 450, 727, 762
HpyF10VI GCNNNNNNNGC 7 cut(s) 158, 164, 173, 485, 500, 524, 665
HpyF3I CTNAG 4 cut(s) 464, 482, 846, 895
HpySE526I ACGT 3 cut(s) 29, 74, 288
Hsp92II CATG 5 cut(s) 256, 598, 616, 690, 1026
HspAI GCGC 2 cut(s) 708, 931
KroI GCCGGC 1 cut(s) 880
KroNI GCCGGC 1 cut(s) 882
Kzo9I GATC 5 cut(s) 327, 580, 700, 978, 997
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 7 cut(s) 101, 181, 411, 419, 665, 750, 956
Lsp1109I GCAGC 5 cut(s) 62, 116, 487, 490, 739
LweI GCATC 3 cut(s) 14, 535, 536
MaeI CTAG 4 cut(s) 429, 515, 551, 680
MaeII ACGT 3 cut(s) 29, 74, 288
MaeIII GTNAC 1 cut(s) 682
MalI GATC 5 cut(s) 329, 582, 702, 980, 999
MbiI CCGCTC 1 cut(s) 135
MboI GATC 5 cut(s) 327, 580, 700, 978, 997
MboII GAAGA 7 cut(s) 130, 133, 136, 139, 142, 199, 767
MfeI CAATTG 2 cut(s) 372, 445
MflI RGATCY 2 cut(s) 700, 997
MhlI GDGCHC 3 cut(s) 116, 416, 470
MlsI TGGCCA 1 cut(s) 573
MluCI AATT 4 cut(s) 372, 445, 575, 1002
MluNI TGGCCA 1 cut(s) 573
MlyI GAGTC 2 cut(s) 224, 827
MmeI TCCRAC 1 cut(s) 279
Mox20I TGGCCA 1 cut(s) 573
MroNI GCCGGC 1 cut(s) 880
MscI TGGCCA 1 cut(s) 573
MseI TTAA 2 cut(s) 909, 1005
MslI CAYNNNNRTG 1 cut(s) 938
Msp20I TGGCCA 1 cut(s) 573
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 4 cut(s) 34, 307, 322, 881
MspR9I CCNGG 4 cut(s) 308, 322, 663, 749
MunI CAATTG 2 cut(s) 372, 445
MvaI CCWGG 2 cut(s) 663, 749
MwoI GCNNNNNNNGC 7 cut(s) 158, 164, 173, 485, 500, 524, 665
NaeI GCCGGC 1 cut(s) 882
NciI CCSGG 2 cut(s) 308, 322
NcoI CCATGG 1 cut(s) 594
NdeII GATC 5 cut(s) 327, 580, 700, 978, 997
NgoMIV GCCGGC 1 cut(s) 880
NheI GCTAGC 1 cut(s) 428
NlaIII CATG 5 cut(s) 256, 598, 616, 690, 1026
NlaIV GGNNCC 3 cut(s) 442, 660, 692
NmeAIII GCCGAG 1 cut(s) 226
NsbI TGCGCA 1 cut(s) 932
NspI RCATGY 3 cut(s) 256, 616, 1026
PaeR7I CTCGAG 1 cut(s) 815
PceI AGGCCT 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 125
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PdiI GCCGGC 1 cut(s) 882
PfeI GAWTC 2 cut(s) 889, 987
PfoI TCCNGGA 1 cut(s) 747
PkrI GCNGC 9 cut(s) 52, 106, 160, 283, 313, 502, 505, 658, 729
PleI GAGTC 2 cut(s) 223, 826
PmaCI CACGTG 1 cut(s) 75
PmlI CACGTG 1 cut(s) 75
PpsI GAGTC 2 cut(s) 223, 826
Ppu21I YACGTR 1 cut(s) 75
PpuMI RGGWCCY 1 cut(s) 235
PshAI GACNNNNGTC 1 cut(s) 366
Psp124BI GAGCTC 2 cut(s) 116, 416
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 2 cut(s) 661, 747
PspCI CACGTG 1 cut(s) 75
PspGI CCWGG 2 cut(s) 661, 747
PspN4I GGNNCC 3 cut(s) 442, 660, 692
PspPI GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
PspPPI RGGWCCY 1 cut(s) 235
PspXI VCTCGAGB 1 cut(s) 815
PstNI CAGNNNCTG 1 cut(s) 481
PsuI RGATCY 2 cut(s) 700, 997
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
RseI CAYNNNNRTG 1 cut(s) 938
SacI GAGCTC 2 cut(s) 116, 416
SapI GCTCTTC 1 cut(s) 125
SaqAI TTAA 2 cut(s) 909, 1005
SatI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 501, 504, 657, 728
Sau3AI GATC 5 cut(s) 327, 580, 700, 978, 997
Sau96I GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
SchI GAGTC 2 cut(s) 224, 827
ScrFI CCNGG 4 cut(s) 308, 322, 663, 749
SduI GDGCHC 3 cut(s) 116, 416, 470
SfaNI GCATC 3 cut(s) 14, 535, 536
Sfr274I CTCGAG 1 cut(s) 815
SinI GGWCC 4 cut(s) 39, 235, 301, 591
SlaI CTCGAG 1 cut(s) 815
SmiMI CAYNNNNRTG 1 cut(s) 938
SmlI CTYRAG 4 cut(s) 507, 806, 815, 830
SmoI CTYRAG 4 cut(s) 507, 806, 815, 830
SpeI ACTAGT 1 cut(s) 679
Sse9I AATT 4 cut(s) 372, 445, 575, 1002
SseBI AGGCCT 1 cut(s) 513
SsiI CCGC 6 cut(s) 107, 135, 158, 282, 311, 656
SspMI CTAG 4 cut(s) 429, 515, 551, 680
SstI GAGCTC 2 cut(s) 116, 416
StuI AGGCCT 1 cut(s) 513
StyD4I CCNGG 4 cut(s) 306, 320, 661, 747
StyI CCWWGG 3 cut(s) 276, 594, 982
TaaI ACNGT 1 cut(s) 345
TaiI ACGT 3 cut(s) 32, 77, 291
TaqI TCGA 4 cut(s) 116, 330, 649, 816
TasI AATT 4 cut(s) 372, 445, 575, 1002
TauI GCSGC 4 cut(s) 161, 284, 314, 659
TfiI GAWTC 2 cut(s) 889, 987
Tru1I TTAA 2 cut(s) 909, 1005
Tru9I TTAA 2 cut(s) 909, 1005
TscAI CASTG 3 cut(s) 159, 183, 891
TseI GCWGC 5 cut(s) 50, 104, 500, 503, 727
TspDTI ATGAA 1 cut(s) 956
TspGWI ACGGA 2 cut(s) 114, 849
TspRI CASTG 3 cut(s) 159, 183, 891
VpaK11BI GGWCC 4 cut(s) 39, 235, 301, 591
XceI RCATGY 3 cut(s) 256, 616, 1026
XcmI CCANNNNNNNNNTGG 1 cut(s) 701
XhoI CTCGAG 1 cut(s) 815
XspI CTAG 4 cut(s) 429, 515, 551, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.