MD12G1017400.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. gTMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011326
Physical Location & Seq
Reverse (-)
1671601 .. 1674896
3296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1017400.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGGGTGTCATATTACAGTGTTATCTGACACGAATCATACGCATGCAGTATTGTTTTTCAGGAGCCACCGACTCTCCAAAGCACTCATACTCTCTCATTCAGTCACTCACTCACTCTGCTGGTAGAGCAGTGCTGCCTCGCAGGTGCCACAGTCTCAGCTGTGCGAGTACAGTCGTGGCCGGAGCGAGCTCCAGGAACAGACTGTTTAGGAGACTGAACGCGGTGGCGGAGACAGCGGCAATGGATGTAGATGTAGTGGAGTTGAAGAAGGGGATTGCTGAGCTTTACGACGAGTCATCTGGCTTGTGGGAGAACATCTGGGGCGACCACATGCACCACGGCTACTACGACCCGAACGCCGACGTTTCGGTCTCCATTCCTGACCATCGCGCCGCCCAGATTCGCATGATCGACGAGGTCCTCCGATTCGCCGGCATTTCTGAGGAGCCCCCAATTAGAATTCCCAAGAATTTGGTGGATGTGGGATGTGGGATCGGAGGGAGCTCCAGATATCTGGCCAGTAAATATGGCGCCAATGCAAAAGGCATCACCCTCAGCCCTGTGCAGGCTGAAAGGGCCAATGCTCTTGCTGCTGCTCAAGGGTTGGCCAACCAGGCTTCATATCAAGTTGCAGATGCTCTGGACCAACCATTTCCTGATGGGGAATTTGATCTGGTATGGTCCATGGAGAGTGGAGAGCACATGCCTGACAAAGCCAAGTTTGTGAACGAGTTGGTTCGGGTTGCTGCCCCAGGAGCAACAATAATAATAGTCACATGGTGCCATAGGGATCTTGGTGCTTCTGAAGAAACCTTGAAGCCAGATGAGAAAAGCCTTCTGGATAGGATATGCAGTTCTTTCTATCTTCCAGCCTGGTGTTCTGCTGCTGATTATCTCAAATTACTCGAGTCCCACTCGCTCGAGGACATCAAGGCGGAAGATTGGTCCCCGTATGTTGCCCCATTTTGGCCTGCAGTTATCCGCTCGGCGTTGACATGGAAGGGTTTCACATCGCTGTTGCGCACCGGAATAAAAACAATCAGAGGAGCATTGGTAATGCCACTGATGATCCAAGGATTCAAGAAGGATCTAATCAAGTATGCAGTGATTACTTGCCGAAAGCCGGAATAA

Protein Analysis

377

Amino Acids

41.4

Weight (kDa)

6.56

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 135 - 274 7.1e-13 Methyltransferase domain
CMAS PF02353 156 - 257 8.3e-11 Mycolic acid cyclopropane synthetase
Methyltransf_31 PF13847 157 - 268 1e-12 Methyltransferase domain
Methyltransf_11 PF08241 159 - 257 2.3e-20 Methyltransferase domain
Methyltransf_25 PF13649 159 - 252 2e-17 Methyltransferase domain
Methyltransf_12 PF08242 159 - 254 1.3e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 132
AasI GACNNNNNNGTC 1 cut(s) 368
Acc16I TGCGCA 1 cut(s) 1022
Acc36I ACCTGC 1 cut(s) 132
AccB1I GGYRCC 3 cut(s) 144, 530, 780
AccBSI CCGCTC 1 cut(s) 984
AccII CGCG 2 cut(s) 221, 390
AciI CCGC 6 cut(s) 221, 227, 236, 393, 935, 982
AclWI GGATC 4 cut(s) 500, 798, 1063, 1095
AcoI YGGCCR 3 cut(s) 177, 516, 606
AcsI RAATTY 3 cut(s) 459, 469, 665
AcuI CTGAAG 1 cut(s) 825
AcyI GRCGYC 1 cut(s) 531
AdeI CACNNNGTG 1 cut(s) 780
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 3 cut(s) 565, 966, 1123
AgsI TTSAA 3 cut(s) 265, 817, 1081
AjnI CCWGG 4 cut(s) 191, 612, 751, 872
AloI GAACNNNNNNTCC 2 cut(s) 838, 870
AluBI AGCT 4 cut(s) 159, 189, 283, 504
AluI AGCT 4 cut(s) 159, 189, 283, 504
Alw21I GWGCWC 3 cut(s) 191, 506, 702
Alw26I GTCTC 4 cut(s) 158, 205, 224, 376
AlwI GGATC 4 cut(s) 500, 798, 1063, 1095
Ama87I CYCGRG 2 cut(s) 905, 920
AoxI GGCC 5 cut(s) 177, 516, 576, 606, 968
ApeKI GCWGC 5 cut(s) 133, 590, 593, 746, 884
ApoI RAATTY 3 cut(s) 459, 469, 665
Asp700I GAANNNNTTC 1 cut(s) 1004
AspLEI GCGC 3 cut(s) 392, 533, 1023
AspS9I GGNCC 5 cut(s) 418, 576, 643, 681, 945
AsuHPI GGTGA 1 cut(s) 541
AvaI CYCGRG 2 cut(s) 905, 920
AvaII GGWCC 4 cut(s) 418, 643, 681, 945
BalI TGGCCA 2 cut(s) 518, 608
BanI GGYRCC 3 cut(s) 144, 530, 780
BanII GRGCYC 3 cut(s) 191, 450, 506
Bbv12I GWGCWC 3 cut(s) 191, 506, 702
BbvCI CCTCAGC 1 cut(s) 554
BbvI GCAGC 5 cut(s) 120, 577, 580, 733, 871
BccI CCATC 2 cut(s) 393, 653
BceAI ACGGC 1 cut(s) 355
BciT130I CCWGG 4 cut(s) 193, 614, 753, 874
BcoDI GTCTC 4 cut(s) 158, 205, 224, 376
BfmI CTRYAG 1 cut(s) 972
BfoI RGCGCY 1 cut(s) 534
BfuAI ACCTGC 1 cut(s) 132
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 7 cut(s) 134, 237, 393, 591, 594, 747, 885
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 7 cut(s) 135, 238, 394, 592, 595, 748, 886
Bme1390I CCNGG 4 cut(s) 193, 614, 753, 874
Bme18I GGWCC 4 cut(s) 418, 643, 681, 945
BmeT110I CYCGRG 2 cut(s) 905, 920
BmgT120I GGNCC 5 cut(s) 418, 576, 643, 681, 945
BmiI GGNNCC 6 cut(s) 64, 146, 447, 532, 782, 947
BmrFI CCNGG 4 cut(s) 193, 614, 753, 874
BmsI GCATC 2 cut(s) 555, 625
BplI GAGNNNNNCTC 2 cut(s) 899, 931
BpmI CTGGAG 2 cut(s) 175, 490
Bpu10I CCTNAGC 1 cut(s) 554
Bpu1102I GCTNAGC 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 582
BsaHI GRCGYC 1 cut(s) 531
BsaI GGTCTC 1 cut(s) 376
BsaJI CCNNGG 4 cut(s) 337, 684, 751, 1072
BsaWI WCCGGW 1 cut(s) 1025
BsaXI ACNNNNNCTCC 4 cut(s) 58, 88, 1038, 1068
Bsc4I CCNNNNNNNGG 3 cut(s) 565, 966, 1123
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 1 cut(s) 519
Bse3DI GCAATG 1 cut(s) 246
BseBI CCWGG 4 cut(s) 193, 614, 753, 874
BseDI CCNNGG 4 cut(s) 337, 684, 751, 1072
BseGI GGATG 3 cut(s) 250, 484, 491
BseLI CCNNNNNNNGG 3 cut(s) 565, 966, 1123
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 4 cut(s) 169, 270, 432, 568
BseNI ACTGG 1 cut(s) 519
BseRI GAGGAG 2 cut(s) 458, 1059
BseXI GCAGC 5 cut(s) 120, 577, 580, 733, 871
BsgI GTGCAG 1 cut(s) 584
Bsh1236I CGCG 2 cut(s) 221, 390
BshFI GGCC 5 cut(s) 179, 518, 578, 608, 970
BshNI GGYRCC 3 cut(s) 144, 530, 780
BsiHKAI GWGCWC 3 cut(s) 191, 506, 702
BsiHKCI CYCGRG 2 cut(s) 905, 920
BsiSI CCGG 4 cut(s) 180, 432, 1026, 1124
BslFI GGGAC 2 cut(s) 895, 931
BslI CCNNNNNNNGG 3 cut(s) 565, 966, 1123
BsmAI GTCTC 4 cut(s) 158, 205, 224, 376
BsmFI GGGAC 2 cut(s) 895, 931
BsnI GGCC 5 cut(s) 179, 518, 578, 608, 970
Bso31I GGTCTC 1 cut(s) 376
BsoBI CYCGRG 2 cut(s) 905, 920
Bsp1286I GDGCHC 4 cut(s) 191, 450, 506, 702
Bsp143I GATC 6 cut(s) 408, 492, 670, 790, 1068, 1087
Bsp1720I GCTNAGC 1 cut(s) 279
Bsp19I CCATGG 1 cut(s) 684
BspACI CCGC 6 cut(s) 221, 227, 236, 393, 935, 982
BspANI GGCC 5 cut(s) 179, 518, 578, 608, 970
BspCNI CTCAG 4 cut(s) 168, 271, 433, 567
BspFNI CGCG 2 cut(s) 221, 390
BspLI GGNNCC 6 cut(s) 64, 146, 447, 532, 782, 947
BspMAI CTGCAG 1 cut(s) 976
BspMI ACCTGC 1 cut(s) 132
BspPI GGATC 4 cut(s) 500, 798, 1063, 1095
BspT107I GGYRCC 3 cut(s) 144, 530, 780
BspTNI GGTCTC 1 cut(s) 376
BsrBI CCGCTC 1 cut(s) 984
BsrDI GCAATG 1 cut(s) 246
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 1 cut(s) 519
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 4 cut(s) 337, 684, 751, 1072
BssMI GATC 6 cut(s) 408, 492, 670, 790, 1068, 1087
BssNI GRCGYC 1 cut(s) 531
BssT1I CCWWGG 2 cut(s) 684, 1072
Bst2UI CCWGG 4 cut(s) 193, 614, 753, 874
Bst4CI ACNGT 4 cut(s) 18, 152, 172, 204
BstACI GRCGYC 1 cut(s) 531
BstC8I GCNNGC 5 cut(s) 44, 187, 433, 567, 972
BstDEI CTNAG 4 cut(s) 155, 279, 441, 554
BstDSI CCRYGG 2 cut(s) 337, 684
BstF5I GGATG 3 cut(s) 250, 484, 491
BstFNI CGCG 2 cut(s) 221, 390
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 3 cut(s) 392, 533, 1023
BstKTI GATC 6 cut(s) 411, 495, 673, 793, 1071, 1090
BstMAI GTCTC 4 cut(s) 158, 205, 224, 376
BstMBI GATC 6 cut(s) 408, 492, 670, 790, 1068, 1087
BstMWI GCNNNNNNNGC 6 cut(s) 125, 233, 575, 590, 614, 755
BstNI CCWGG 4 cut(s) 193, 614, 753, 874
BstNSI RCATGY 3 cut(s) 46, 334, 706
BstSCI CCNGG 4 cut(s) 191, 612, 751, 872
BstSFI CTRYAG 1 cut(s) 972
BstUI CGCG 2 cut(s) 221, 390
BstV1I GCAGC 5 cut(s) 120, 577, 580, 733, 871
BstX2I RGATCY 2 cut(s) 790, 1087
BstXI CCANNNNNNTGG 2 cut(s) 472, 514
BstYI RGATCY 2 cut(s) 790, 1087
BsuRI GGCC 5 cut(s) 179, 518, 578, 608, 970
BtgI CCRYGG 2 cut(s) 337, 684
BtgZI GCGATG 2 cut(s) 371, 996
BtsCI GGATG 3 cut(s) 250, 484, 491
BtsI GCAGTG 2 cut(s) 135, 1110
BtsIMutI CAGTG 4 cut(s) 23, 135, 1061, 1110
BveI ACCTGC 1 cut(s) 132
Cac8I GCNNGC 5 cut(s) 44, 187, 433, 567, 972
CfoI GCGC 3 cut(s) 392, 533, 1023
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 5 cut(s) 418, 576, 643, 681, 945
Csp6I GTAC 1 cut(s) 168
CviAII CATG 7 cut(s) 43, 331, 406, 685, 703, 777, 996
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 4 cut(s) 155, 279, 441, 554
DinI GGCGCC 1 cut(s) 532
DpnI GATC 6 cut(s) 410, 494, 672, 792, 1070, 1089
DpnII GATC 6 cut(s) 408, 492, 670, 790, 1068, 1087
DraIII CACNNNGTG 1 cut(s) 780
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 3 cut(s) 177, 516, 606
EciI GGCGGA 2 cut(s) 242, 950
Ecl136II GAGCTC 2 cut(s) 189, 504
Eco130I CCWWGG 2 cut(s) 684, 1072
Eco24I GRGCYC 3 cut(s) 191, 450, 506
Eco31I GGTCTC 1 cut(s) 376
Eco32I GATATC 1 cut(s) 512
Eco47I GGWCC 4 cut(s) 418, 643, 681, 945
Eco53kI GAGCTC 2 cut(s) 189, 504
Eco57I CTGAAG 1 cut(s) 825
Eco88I CYCGRG 2 cut(s) 905, 920
EcoICRI GAGCTC 2 cut(s) 189, 504
EcoO109I RGGNCCY 1 cut(s) 418
EcoRI GAATTC 1 cut(s) 459
EcoRII CCWGG 4 cut(s) 191, 612, 751, 872
EcoRV GATATC 1 cut(s) 512
EcoT14I CCWWGG 2 cut(s) 684, 1072
EcoT38I GRGCYC 3 cut(s) 191, 450, 506
EgeI GGCGCC 1 cut(s) 532
EheI GGCGCC 1 cut(s) 532
ErhI CCWWGG 2 cut(s) 684, 1072
FaeI CATG 7 cut(s) 46, 334, 409, 688, 706, 780, 999
FaqI GGGAC 2 cut(s) 895, 931
FatI CATG 7 cut(s) 42, 330, 405, 684, 702, 776, 995
Fnu4HI GCNGC 7 cut(s) 134, 237, 393, 591, 594, 747, 885
FokI GGATG 3 cut(s) 257, 491, 498
FriOI GRGCYC 3 cut(s) 191, 450, 506
Fsp4HI GCNGC 7 cut(s) 134, 237, 393, 591, 594, 747, 885
FspI TGCGCA 1 cut(s) 1022
GlaI GCGC 3 cut(s) 391, 532, 1022
GluI GCNGC 7 cut(s) 134, 237, 393, 591, 594, 747, 885
GsuI CTGGAG 2 cut(s) 175, 490
HaeII RGCGCY 1 cut(s) 534
HaeIII GGCC 5 cut(s) 179, 518, 578, 608, 970
HapII CCGG 4 cut(s) 180, 432, 1026, 1124
HhaI GCGC 3 cut(s) 392, 533, 1023
Hin1I GRCGYC 1 cut(s) 531
Hin1II CATG 7 cut(s) 46, 334, 409, 688, 706, 780, 999
Hin6I GCGC 3 cut(s) 390, 531, 1021
HinP1I GCGC 3 cut(s) 390, 531, 1021
HincII GTYRAC 1 cut(s) 993
HindII GTYRAC 1 cut(s) 993
HinfI GANTC 7 cut(s) 33, 71, 293, 400, 426, 908, 1077
HpaII CCGG 4 cut(s) 180, 432, 1026, 1124
HphI GGTGA 1 cut(s) 541
Hpy166II GTNNAC 2 cut(s) 727, 993
Hpy188I TCNGA 6 cut(s) 27, 425, 442, 497, 805, 1043
Hpy188III TCNNGA 7 cut(s) 60, 380, 507, 641, 656, 839, 1081
Hpy8I GTNNAC 2 cut(s) 727, 993
Hpy99I CGWCG 3 cut(s) 293, 365, 416
HpyAV CCTTC 4 cut(s) 262, 845, 994, 1078
HpyCH4III ACNGT 4 cut(s) 18, 152, 172, 204
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 8 cut(s) 46, 334, 539, 565, 632, 852, 974, 1103
HpyF10VI GCNNNNNNNGC 6 cut(s) 125, 233, 575, 590, 614, 755
HpyF3I CTNAG 4 cut(s) 155, 279, 441, 554
HpySE526I ACGT 1 cut(s) 363
Hsp92I GRCGYC 1 cut(s) 531
Hsp92II CATG 7 cut(s) 46, 334, 409, 688, 706, 780, 999
HspAI GCGC 3 cut(s) 390, 531, 1021
KasI GGCGCC 1 cut(s) 530
KroI GCCGGC 1 cut(s) 431
KroNI GCCGGC 1 cut(s) 433
Kzo9I GATC 6 cut(s) 408, 492, 670, 790, 1068, 1087
LmnI GCTCC 8 cut(s) 62, 182, 194, 445, 501, 509, 755, 1046
Lsp1109I GCAGC 5 cut(s) 120, 577, 580, 733, 871
LweI GCATC 2 cut(s) 555, 625
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 2 cut(s) 102, 772
MalI GATC 6 cut(s) 410, 494, 672, 792, 1070, 1089
MbiI CCGCTC 1 cut(s) 984
MboI GATC 6 cut(s) 408, 492, 670, 790, 1068, 1087
MboII GAAGA 4 cut(s) 277, 818, 857, 950
MflI RGATCY 2 cut(s) 790, 1087
MhlI GDGCHC 4 cut(s) 191, 450, 506, 702
MlsI TGGCCA 2 cut(s) 518, 608
MluCI AATT 5 cut(s) 453, 459, 469, 665, 899
MluNI TGGCCA 2 cut(s) 518, 608
Mly113I GGCGCC 1 cut(s) 531
MlyI GAGTC 3 cut(s) 65, 302, 917
MnlI CCTC 8 cut(s) 147, 409, 431, 436, 491, 563, 916, 1037
Mox20I TGGCCA 2 cut(s) 518, 608
MroNI GCCGGC 1 cut(s) 431
MroXI GAANNNNTTC 1 cut(s) 1004
MscI TGGCCA 2 cut(s) 518, 608
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 2 cut(s) 518, 608
MspA1I CMGCKG 2 cut(s) 159, 236
MspI CCGG 4 cut(s) 180, 432, 1026, 1124
MspR9I CCNGG 4 cut(s) 193, 614, 753, 874
MvaI CCWGG 4 cut(s) 193, 614, 753, 874
MvnI CGCG 2 cut(s) 221, 390
MwoI GCNNNNNNNGC 6 cut(s) 125, 233, 575, 590, 614, 755
NaeI GCCGGC 1 cut(s) 433
NarI GGCGCC 1 cut(s) 531
NcoI CCATGG 1 cut(s) 684
NdeII GATC 6 cut(s) 408, 492, 670, 790, 1068, 1087
NgoMIV GCCGGC 1 cut(s) 431
NlaIII CATG 7 cut(s) 46, 334, 409, 688, 706, 780, 999
NlaIV GGNNCC 6 cut(s) 64, 146, 447, 532, 782, 947
NmeAIII GCCGAG 1 cut(s) 965
NmuCI GTSAC 2 cut(s) 102, 772
NsbI TGCGCA 1 cut(s) 1022
NspI RCATGY 3 cut(s) 46, 334, 706
PaeI GCATGC 1 cut(s) 46
PaeR7I CTCGAG 2 cut(s) 905, 920
PaqCI CACCTGC 1 cut(s) 132
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PdiI GCCGGC 1 cut(s) 433
PdmI GAANNNNTTC 1 cut(s) 1004
PfeI GAWTC 4 cut(s) 33, 400, 426, 1077
PflFI GACNNNGTC 1 cut(s) 416
PfoI TCCNGGA 1 cut(s) 191
PkrI GCNGC 7 cut(s) 135, 238, 394, 592, 595, 748, 886
PleI GAGTC 3 cut(s) 65, 301, 916
PluTI GGCGCC 1 cut(s) 534
PpsI GAGTC 3 cut(s) 65, 301, 916
PpuMI RGGWCCY 1 cut(s) 418
Psp124BI GAGCTC 2 cut(s) 191, 506
Psp5II RGGWCCY 1 cut(s) 418
Psp6I CCWGG 4 cut(s) 191, 612, 751, 872
PspGI CCWGG 4 cut(s) 191, 612, 751, 872
PspN4I GGNNCC 6 cut(s) 64, 146, 447, 532, 782, 947
PspPI GGNCC 5 cut(s) 418, 576, 643, 681, 945
PspPPI RGGWCCY 1 cut(s) 418
PspXI VCTCGAGB 2 cut(s) 905, 920
PstI CTGCAG 1 cut(s) 976
PsuI RGATCY 2 cut(s) 790, 1087
PsyI GACNNNGTC 1 cut(s) 416
PvuII CAGCTG 1 cut(s) 159
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
RseI CAYNNNNRTG 1 cut(s) 41
SacI GAGCTC 2 cut(s) 191, 506
SatI GCNGC 7 cut(s) 134, 237, 393, 591, 594, 747, 885
Sau3AI GATC 6 cut(s) 408, 492, 670, 790, 1068, 1087
Sau96I GGNCC 5 cut(s) 418, 576, 643, 681, 945
SchI GAGTC 3 cut(s) 65, 302, 917
ScrFI CCNGG 4 cut(s) 193, 614, 753, 874
SduI GDGCHC 4 cut(s) 191, 450, 506, 702
SetI ASST 8 cut(s) 146, 161, 191, 285, 366, 420, 506, 815
SfaNI GCATC 2 cut(s) 555, 625
SfcI CTRYAG 1 cut(s) 972
SfoI GGCGCC 1 cut(s) 532
Sfr274I CTCGAG 2 cut(s) 905, 920
SinI GGWCC 4 cut(s) 418, 643, 681, 945
SlaI CTCGAG 2 cut(s) 905, 920
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 3 cut(s) 597, 905, 920
SmoI CTYRAG 3 cut(s) 597, 905, 920
SphI GCATGC 1 cut(s) 46
Sse9I AATT 5 cut(s) 453, 459, 469, 665, 899
SsiI CCGC 6 cut(s) 221, 227, 236, 393, 935, 982
SspDI GGCGCC 1 cut(s) 530
SstI GAGCTC 2 cut(s) 191, 506
StyD4I CCNGG 4 cut(s) 191, 612, 751, 872
StyI CCWWGG 2 cut(s) 684, 1072
TaaI ACNGT 4 cut(s) 18, 152, 172, 204
TaiI ACGT 1 cut(s) 366
TaqI TCGA 3 cut(s) 411, 906, 921
TaqII GACCGA 1 cut(s) 358
TasI AATT 5 cut(s) 453, 459, 469, 665, 899
TatI WGTACW 1 cut(s) 167
TauI GCSGC 2 cut(s) 239, 395
TfiI GAWTC 4 cut(s) 33, 400, 426, 1077
TscAI CASTG 4 cut(s) 23, 135, 1068, 1110
TseFI GTSAC 2 cut(s) 102, 772
TseI GCWGC 5 cut(s) 133, 590, 593, 746, 884
Tsp45I GTSAC 2 cut(s) 102, 772
TspDTI ATGAA 1 cut(s) 609
TspRI CASTG 4 cut(s) 23, 135, 1068, 1110
Tth111I GACNNNGTC 1 cut(s) 416
VpaK11BI GGWCC 4 cut(s) 418, 643, 681, 945
XapI RAATTY 3 cut(s) 459, 469, 665
XceI RCATGY 3 cut(s) 46, 334, 706
XcmI CCANNNNNNNNNTGG 2 cut(s) 472, 791
XhoI CTCGAG 2 cut(s) 905, 920
XmnI GAANNNNTTC 1 cut(s) 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.