MD14G1015100.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. gTMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011326
Physical Location & Seq
Reverse (-)
1330025 .. 1333433
3409 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1015100.v1.1.491

Sequence Viewer

Length: 984 bp
ATGTGCTACCGTCTCAATCGTGCGAGTACAGTCGTGGTCGGAGCGAACTCCAGGAACAGAGGATTGAGGAGACTGAACGCGGTGGCGGAGACGGCAGCGATGGAGGCAGTGGAGCTGAAGAAGGGGATTGCTGAGCTGTACGACGAGTCGTCTGGCTTGTGGGAGAACATCTGGGGCGACCACATGCACCACGGATACTACGACCCAGACGCCGACGTTTCGGTCTCCATTCCCGACCATCGCGCCGCCCAGATTCGCATGATCGACGAGGTCCTCCGATTCGCCGGCATTTCTGAGGAGCCCCCAACTAGACTTCCTAAGAATTTGGTGGATGTGGGATGTGGGATCGGAGGGAGCTCCAGATATCTGGCTAGTAAATATGGGGCCAATGCCAAAGGCATCACCCTCAGCCCTGTGCAGGCTGAAAGGGCCAATGCTCTTGCTGCTGCCCAAGGGTTAGCCAATCAGGCTTCGTTTCAAGTTGCAGATGCTCTGGACCAACCATTTCCTGACGGGGAATTTGATCTGGTATGGTCCATGGAGAGTGGTGAGCACATGCCTGACAAAGCCAAGTTTGTGAATGAGTTGGTTCGAGTTGCTGCACCAGGAGCAACAATAATTATAGTCACATGGTGCCACAGGGATCTTGGTGCTTCTGAAAAAACCTTGAAGCCAGATGAGAAGAATCTCCTGGACAGGATATGCAATTCATTCTATCTTCCAGCCTGGTGTTCCACTGCTGATTATCTCAAGTTACTCGAGTCCCACTCGCTCGAGGACATCAAGGCGGAAGATTGGTCCCCTTATGTTGCCCCGTTTTGGCCTGCAGTTATCCGCTCGGCATTGACATGGAAGGGTTTTACATCGCTGTTAGGCACCGGGATAAAAACAATAAGAGGAGCATTGGTAATGCCATTGATGATCCAAGGATTCAAGAAGGACCTAATCAAGTATTCGGTGATTACGTGCCGAAAGCCGGAATAA

Protein Analysis

328

Amino Acids

36.01

Weight (kDa)

6.02

Isoelectric Point (pI)

28.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 86 - 221 1.6e-13 Methyltransferase domain
CMAS PF02353 107 - 208 3.8e-11 Mycolic acid cyclopropane synthetase
Methyltransf_31 PF13847 108 - 226 1.7e-14 Methyltransferase domain
Methyltransf_11 PF08241 110 - 208 1.6e-20 Methyltransferase domain
Methyltransf_25 PF13649 110 - 203 5.4e-18 Methyltransferase domain
Methyltransf_12 PF08242 110 - 205 7.3e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
AccB1I GGYRCC 2 cut(s) 633, 875
AccBSI CCGCTC 1 cut(s) 837
AccII CGCG 2 cut(s) 80, 243
AciI CCGC 5 cut(s) 80, 86, 246, 788, 835
AclWI GGATC 3 cut(s) 353, 651, 916
AcsI RAATTY 2 cut(s) 322, 518
AcuI CTGAAG 1 cut(s) 137
AcyI GRCGYC 1 cut(s) 210
AdeI CACNNNGTG 1 cut(s) 633
AfaI GTAC 2 cut(s) 28, 140
AfiI CCNNNNNNNGG 3 cut(s) 418, 819, 976
AgsI TTSAA 3 cut(s) 479, 670, 934
AhdI GACNNNNNGTC 1 cut(s) 148
AjnI CCWGG 4 cut(s) 50, 604, 690, 725
AluBI AGCT 3 cut(s) 115, 136, 357
AluI AGCT 3 cut(s) 115, 136, 357
Alw21I GWGCWC 2 cut(s) 359, 555
Alw26I GTCTC 4 cut(s) 17, 64, 83, 229
AlwI GGATC 3 cut(s) 353, 651, 916
Ama87I CYCGRG 2 cut(s) 758, 773
AoxI GGCC 3 cut(s) 384, 429, 821
ApeKI GCWGC 4 cut(s) 95, 443, 446, 599
ApoI RAATTY 2 cut(s) 322, 518
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 7 cut(s) 271, 384, 429, 496, 534, 798, 940
AsuC2I CCSGG 1 cut(s) 880
AsuHPI GGTGA 3 cut(s) 394, 560, 970
AvaI CYCGRG 2 cut(s) 758, 773
AvaII GGWCC 5 cut(s) 271, 496, 534, 798, 940
BanI GGYRCC 2 cut(s) 633, 875
BanII GRGCYC 2 cut(s) 303, 359
Bbv12I GWGCWC 2 cut(s) 359, 555
BbvCI CCTCAGC 1 cut(s) 407
BbvI GCAGC 4 cut(s) 107, 430, 433, 586
BccI CCATC 2 cut(s) 94, 246
BceAI ACGGC 1 cut(s) 108
BciT130I CCWGG 4 cut(s) 52, 606, 692, 727
BciVI GTATCC 1 cut(s) 188
BcnI CCSGG 1 cut(s) 880
BcoDI GTCTC 4 cut(s) 17, 64, 83, 229
BfaI CTAG 2 cut(s) 309, 372
BfmI CTRYAG 1 cut(s) 825
BfuI GTATCC 1 cut(s) 188
BglI GCCNNNNNGGC 1 cut(s) 467
BisI GCNGC 5 cut(s) 96, 246, 444, 447, 600
BlpI GCTNAGC 1 cut(s) 132
BlsI GCNGC 5 cut(s) 97, 247, 445, 448, 601
Bme1390I CCNGG 5 cut(s) 52, 606, 692, 727, 880
Bme18I GGWCC 5 cut(s) 271, 496, 534, 798, 940
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 2 cut(s) 758, 773
BmgT120I GGNCC 7 cut(s) 271, 384, 429, 496, 534, 798, 940
BmiI GGNNCC 5 cut(s) 300, 385, 635, 800, 877
BmrFI CCNGG 5 cut(s) 52, 606, 692, 727, 880
BmsI GCATC 2 cut(s) 408, 478
BplI GAGNNNNNCTC 2 cut(s) 752, 784
BpmI CTGGAG 2 cut(s) 34, 343
Bpu10I CCTNAGC 1 cut(s) 407
Bpu1102I GCTNAGC 1 cut(s) 132
BpuEI CTTGAG 1 cut(s) 734
BpuMI CCSGG 1 cut(s) 880
BsaAI YACGTR 1 cut(s) 966
BsaHI GRCGYC 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 4 cut(s) 190, 451, 537, 925
BsaXI ACNNNNNCTCC 2 cut(s) 891, 921
Bsc4I CCNNNNNNNGG 3 cut(s) 418, 819, 976
Bse118I RCCGGY 1 cut(s) 284
BseBI CCWGG 4 cut(s) 52, 606, 692, 727
BseDI CCNNGG 4 cut(s) 190, 451, 537, 925
BseGI GGATG 2 cut(s) 337, 344
BseLI CCNNNNNNNGG 3 cut(s) 418, 819, 976
BseMII CTCAG 3 cut(s) 123, 285, 421
BseRI GAGGAG 3 cut(s) 82, 311, 912
BseXI GCAGC 4 cut(s) 107, 430, 433, 586
BsgI GTGCAG 2 cut(s) 437, 585
Bsh1236I CGCG 2 cut(s) 80, 243
BshFI GGCC 3 cut(s) 386, 431, 823
BshNI GGYRCC 2 cut(s) 633, 875
BsiHKAI GWGCWC 2 cut(s) 359, 555
BsiHKCI CYCGRG 2 cut(s) 758, 773
BsiSI CCGG 3 cut(s) 285, 879, 977
BslFI GGGAC 2 cut(s) 748, 784
BslI CCNNNNNNNGG 3 cut(s) 418, 819, 976
BsmAI GTCTC 4 cut(s) 17, 64, 83, 229
BsmBI CGTCTC 2 cut(s) 17, 83
BsmFI GGGAC 2 cut(s) 748, 784
BsnI GGCC 3 cut(s) 386, 431, 823
Bso31I GGTCTC 1 cut(s) 229
BsoBI CYCGRG 2 cut(s) 758, 773
Bsp1286I GDGCHC 3 cut(s) 303, 359, 555
Bsp143I GATC 5 cut(s) 261, 345, 523, 643, 921
Bsp1720I GCTNAGC 1 cut(s) 132
Bsp19I CCATGG 1 cut(s) 537
BspACI CCGC 5 cut(s) 80, 86, 246, 788, 835
BspANI GGCC 3 cut(s) 386, 431, 823
BspCNI CTCAG 3 cut(s) 124, 286, 420
BspFNI CGCG 2 cut(s) 80, 243
BspLI GGNNCC 5 cut(s) 300, 385, 635, 800, 877
BspMAI CTGCAG 1 cut(s) 829
BspPI GGATC 3 cut(s) 353, 651, 916
BspT107I GGYRCC 2 cut(s) 633, 875
BspTNI GGTCTC 1 cut(s) 229
BsrBI CCGCTC 1 cut(s) 837
BsrFI RCCGGY 1 cut(s) 284
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 4 cut(s) 190, 451, 537, 925
BssMI GATC 5 cut(s) 261, 345, 523, 643, 921
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 3 cut(s) 451, 537, 925
Bst2UI CCWGG 4 cut(s) 52, 606, 692, 727
Bst4CI ACNGT 2 cut(s) 11, 31
BstACI GRCGYC 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 966
BstC8I GCNNGC 3 cut(s) 286, 420, 825
BstDEI CTNAG 4 cut(s) 132, 294, 318, 407
BstDSI CCRYGG 2 cut(s) 190, 537
BstF5I GGATG 2 cut(s) 337, 344
BstFNI CGCG 2 cut(s) 80, 243
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 5 cut(s) 264, 348, 526, 646, 924
BstMAI GTCTC 4 cut(s) 17, 64, 83, 229
BstMBI GATC 5 cut(s) 261, 345, 523, 643, 921
BstMWI GCNNNNNNNGC 6 cut(s) 92, 104, 428, 443, 467, 608
BstNI CCWGG 4 cut(s) 52, 606, 692, 727
BstNSI RCATGY 2 cut(s) 187, 559
BstSCI CCNGG 5 cut(s) 50, 604, 690, 725, 878
BstSFI CTRYAG 1 cut(s) 825
BstUI CGCG 2 cut(s) 80, 243
BstV1I GCAGC 4 cut(s) 107, 430, 433, 586
BstX2I RGATCY 1 cut(s) 643
BstXI CCANNNNNNTGG 1 cut(s) 367
BstYI RGATCY 1 cut(s) 643
BsuI GTATCC 1 cut(s) 188
BsuRI GGCC 3 cut(s) 386, 431, 823
BtgI CCRYGG 2 cut(s) 190, 537
BtgZI GCGATG 3 cut(s) 113, 224, 849
BtsCI GGATG 2 cut(s) 337, 344
BtsI GCAGTG 2 cut(s) 114, 735
BtsIMutI CAGTG 2 cut(s) 114, 735
Cac8I GCNNGC 3 cut(s) 286, 420, 825
CfoI GCGC 1 cut(s) 245
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 7 cut(s) 271, 384, 429, 496, 534, 798, 940
CseI GACGC 1 cut(s) 218
Csp6I GTAC 2 cut(s) 27, 139
CviAII CATG 6 cut(s) 184, 259, 538, 556, 630, 849
CviQI GTAC 2 cut(s) 27, 139
DdeI CTNAG 4 cut(s) 132, 294, 318, 407
DpnI GATC 5 cut(s) 263, 347, 525, 645, 923
DpnII GATC 5 cut(s) 261, 345, 523, 643, 921
DraIII CACNNNGTG 1 cut(s) 633
DrdI GACNNNNNNGTC 1 cut(s) 221
DriI GACNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 221
Eam1105I GACNNNNNGTC 1 cut(s) 148
EciI GGCGGA 2 cut(s) 101, 803
Ecl136II GAGCTC 1 cut(s) 357
Eco130I CCWWGG 3 cut(s) 451, 537, 925
Eco24I GRGCYC 2 cut(s) 303, 359
Eco31I GGTCTC 1 cut(s) 229
Eco32I GATATC 1 cut(s) 365
Eco47I GGWCC 5 cut(s) 271, 496, 534, 798, 940
Eco53kI GAGCTC 1 cut(s) 357
Eco57I CTGAAG 1 cut(s) 137
Eco88I CYCGRG 2 cut(s) 758, 773
EcoICRI GAGCTC 1 cut(s) 357
EcoO109I RGGNCCY 2 cut(s) 271, 940
EcoRII CCWGG 4 cut(s) 50, 604, 690, 725
EcoRV GATATC 1 cut(s) 365
EcoT14I CCWWGG 3 cut(s) 451, 537, 925
EcoT38I GRGCYC 2 cut(s) 303, 359
ErhI CCWWGG 3 cut(s) 451, 537, 925
Esp3I CGTCTC 2 cut(s) 17, 83
FaeI CATG 6 cut(s) 187, 262, 541, 559, 633, 852
FaqI GGGAC 2 cut(s) 748, 784
FatI CATG 6 cut(s) 183, 258, 537, 555, 629, 848
Fnu4HI GCNGC 5 cut(s) 96, 246, 444, 447, 600
FokI GGATG 2 cut(s) 344, 351
FriOI GRGCYC 2 cut(s) 303, 359
Fsp4HI GCNGC 5 cut(s) 96, 246, 444, 447, 600
FspBI CTAG 2 cut(s) 309, 372
GlaI GCGC 1 cut(s) 244
GluI GCNGC 5 cut(s) 96, 246, 444, 447, 600
GsuI CTGGAG 2 cut(s) 34, 343
HaeIII GGCC 3 cut(s) 386, 431, 823
HapII CCGG 3 cut(s) 285, 879, 977
HgaI GACGC 1 cut(s) 218
HhaI GCGC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 6 cut(s) 187, 262, 541, 559, 633, 852
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HinfI GANTC 6 cut(s) 146, 253, 279, 685, 761, 930
HpaII CCGG 3 cut(s) 285, 879, 977
HphI GGTGA 3 cut(s) 394, 560, 970
Hpy188I TCNGA 5 cut(s) 41, 278, 295, 350, 658
Hpy188III TCNNGA 5 cut(s) 233, 360, 494, 509, 934
Hpy99I CGWCG 3 cut(s) 146, 218, 269
HpyAV CCTTC 3 cut(s) 115, 847, 931
HpyCH4III ACNGT 2 cut(s) 11, 31
HpyCH4IV ACGT 2 cut(s) 216, 965
HpyCH4V TGCA 6 cut(s) 187, 418, 485, 602, 705, 827
HpyF10VI GCNNNNNNNGC 6 cut(s) 92, 104, 428, 443, 467, 608
HpyF3I CTNAG 4 cut(s) 132, 294, 318, 407
HpySE526I ACGT 2 cut(s) 216, 965
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 6 cut(s) 187, 262, 541, 559, 633, 852
HspAI GCGC 1 cut(s) 243
KroI GCCGGC 1 cut(s) 284
KroNI GCCGGC 1 cut(s) 286
Kzo9I GATC 5 cut(s) 261, 345, 523, 643, 921
LmnI GCTCC 7 cut(s) 41, 112, 298, 354, 362, 608, 899
Lsp1109I GCAGC 4 cut(s) 107, 430, 433, 586
LweI GCATC 2 cut(s) 408, 478
MaeI CTAG 2 cut(s) 309, 372
MaeII ACGT 2 cut(s) 216, 965
MaeIII GTNAC 2 cut(s) 625, 753
MalI GATC 5 cut(s) 263, 347, 525, 645, 923
MbiI CCGCTC 1 cut(s) 837
MboI GATC 5 cut(s) 261, 345, 523, 643, 921
MboII GAAGA 4 cut(s) 130, 694, 710, 803
MflI RGATCY 1 cut(s) 643
MhlI GDGCHC 3 cut(s) 303, 359, 555
MluCI AATT 4 cut(s) 322, 518, 618, 706
MlyI GAGTC 2 cut(s) 155, 770
MmeI TCCRAC 1 cut(s) 19
MroNI GCCGGC 1 cut(s) 284
MslI CAYNNNNRTG 1 cut(s) 847
MspI CCGG 3 cut(s) 285, 879, 977
MspR9I CCNGG 5 cut(s) 52, 606, 692, 727, 880
MvaI CCWGG 4 cut(s) 52, 606, 692, 727
MvnI CGCG 2 cut(s) 80, 243
MwoI GCNNNNNNNGC 6 cut(s) 92, 104, 428, 443, 467, 608
NaeI GCCGGC 1 cut(s) 286
NciI CCSGG 1 cut(s) 880
NcoI CCATGG 1 cut(s) 537
NdeII GATC 5 cut(s) 261, 345, 523, 643, 921
NgoMIV GCCGGC 1 cut(s) 284
NlaIII CATG 6 cut(s) 187, 262, 541, 559, 633, 852
NlaIV GGNNCC 5 cut(s) 300, 385, 635, 800, 877
NmeAIII GCCGAG 1 cut(s) 818
NmuCI GTSAC 1 cut(s) 625
NspI RCATGY 2 cut(s) 187, 559
PaeR7I CTCGAG 2 cut(s) 758, 773
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 962
PdiI GCCGGC 1 cut(s) 286
PfeI GAWTC 4 cut(s) 253, 279, 685, 930
PflFI GACNNNGTC 1 cut(s) 269
PfoI TCCNGGA 2 cut(s) 50, 690
PkrI GCNGC 5 cut(s) 97, 247, 445, 448, 601
PleI GAGTC 2 cut(s) 154, 769
PpsI GAGTC 2 cut(s) 154, 769
Ppu21I YACGTR 1 cut(s) 966
PpuMI RGGWCCY 2 cut(s) 271, 940
Psp124BI GAGCTC 1 cut(s) 359
Psp5II RGGWCCY 2 cut(s) 271, 940
Psp6I CCWGG 4 cut(s) 50, 604, 690, 725
PspGI CCWGG 4 cut(s) 50, 604, 690, 725
PspN4I GGNNCC 5 cut(s) 300, 385, 635, 800, 877
PspPI GGNCC 7 cut(s) 271, 384, 429, 496, 534, 798, 940
PspPPI RGGWCCY 2 cut(s) 271, 940
PspXI VCTCGAGB 2 cut(s) 758, 773
PstI CTGCAG 1 cut(s) 829
PsuI RGATCY 1 cut(s) 643
PsyI GACNNNGTC 1 cut(s) 269
RsaI GTAC 2 cut(s) 28, 140
RsaNI GTAC 2 cut(s) 27, 139
RseI CAYNNNNRTG 1 cut(s) 847
SacI GAGCTC 1 cut(s) 359
SatI GCNGC 5 cut(s) 96, 246, 444, 447, 600
Sau3AI GATC 5 cut(s) 261, 345, 523, 643, 921
Sau96I GGNCC 7 cut(s) 271, 384, 429, 496, 534, 798, 940
SchI GAGTC 2 cut(s) 155, 770
ScrFI CCNGG 5 cut(s) 52, 606, 692, 727, 880
SduI GDGCHC 3 cut(s) 303, 359, 555
SetI ASST 8 cut(s) 117, 138, 219, 273, 359, 668, 945, 968
SfaNI GCATC 2 cut(s) 408, 478
SfcI CTRYAG 1 cut(s) 825
Sfr274I CTCGAG 2 cut(s) 758, 773
SinI GGWCC 5 cut(s) 271, 496, 534, 798, 940
SlaI CTCGAG 2 cut(s) 758, 773
SmiMI CAYNNNNRTG 1 cut(s) 847
SmlI CTYRAG 3 cut(s) 749, 758, 773
SmoI CTYRAG 3 cut(s) 749, 758, 773
Sse9I AATT 4 cut(s) 322, 518, 618, 706
SsiI CCGC 5 cut(s) 80, 86, 246, 788, 835
SspMI CTAG 2 cut(s) 309, 372
SstI GAGCTC 1 cut(s) 359
StyD4I CCNGG 5 cut(s) 50, 604, 690, 725, 878
StyI CCWWGG 3 cut(s) 451, 537, 925
TaaI ACNGT 2 cut(s) 11, 31
TaiI ACGT 2 cut(s) 219, 968
TaqI TCGA 4 cut(s) 264, 592, 759, 774
TaqII GACCGA 1 cut(s) 211
TasI AATT 4 cut(s) 322, 518, 618, 706
TatI WGTACW 1 cut(s) 26
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 4 cut(s) 253, 279, 685, 930
TscAI CASTG 2 cut(s) 114, 742
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 4 cut(s) 95, 443, 446, 599
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 1 cut(s) 699
TspGWI ACGGA 1 cut(s) 207
TspRI CASTG 2 cut(s) 114, 742
Tth111I GACNNNGTC 1 cut(s) 269
VpaK11BI GGWCC 5 cut(s) 271, 496, 534, 798, 940
XapI RAATTY 2 cut(s) 322, 518
XceI RCATGY 2 cut(s) 187, 559
XcmI CCANNNNNNNNNTGG 1 cut(s) 644
XhoI CTCGAG 2 cut(s) 758, 773
XspI CTAG 2 cut(s) 309, 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.