FvH4_6g17810
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. gTMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011326
Physical Location & Seq
Reverse (-)
11680263 .. 11684463
4201 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17810.t1

Sequence Viewer

Length: 1044 bp
ATGGCATCGACGAGACTGAGCTACCAGACGTGCGGGTGGTCCTCGTCGTCGGCAGCAGGAACTACTACTACTACTCTCCTTCCTCCTCGCACCTGTCTCGGTGTGGTTCGTGCGAGCGCGAAGAAGAGAGCGTTTGAGCTTAGAGTGGTGGCGGAGACGGCGATGGAGGCGGTGGAGCTGAAGAAGGACATAGCGGAGTTCTACGACGAGTCGTCTGCGTTGTGGGAGGATCTCTGGGGCGACCATATGCACCACGGCTTCTACGACCCGAAAGCCGATGACGTTTCGGTTTCGGACCACCGCGCGGCCCAGACCCGGATGATCGACGAGGTGCTCCGATTCGCCGGCATTTCTGAGAAGTTGCCAATAGGGAAGGGGAAGAATGTGGTGGATGTTGGGTGTGGGATTGGAGGGAGCTCCAGGTATCTGGCTAGCAAATATGGAGCAAATTGCAAAGGGATTACTCTGAGTCCTGTGCAGGCTCAAAGGGCCAATGCTCTTGCTGCTGCCCAAGGCCTGGCCAATAAGGCATCATTTCAAGTTGCTGATGCTCTTGCACAACCATTTCCTGATGGCCAATTTGATCTGGTTTGGTCCATGGAGAGTGGGGAACACATGCCTGACAAAGCAAAGTTTGTTGATGAGCTGGTTCGAGTTGCTGCCCCTGGAGCAACGATAATACTTGTTACATGGTGCCACAGGGATCTTGGTCCATCTGAACAGTCCTTGAAGCCAGATGAGAAGAAGCTCTTGGACAAGATATGCAATGCTTTCTATCTTCCAGCTTGGTGTTCTACTGCTGACTACCTCAAGCTACTCGAGTCCTACTCACTCGAGGATATCAAGGCTGAGGACTGGTCTCCATATGTTGCCCCATTTTGGCCGGCAGTGATTCGCTCAGCATTGACATTTAAGGGTTTCTTTTCTCTGTTGCGCACAGGAACAAAAACAATAAGAGGAGCATTGGCAATGCCACTGATGATCCAAGGATTCAAGAAGGATCTAATCAAATATTCCATTATTACATGCCGAAAACCTGAATGA

Protein Analysis

348

Amino Acids

38.07

Weight (kDa)

7.55

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 62 - 225 3.8e-14 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 102 - 245 2.3e-14 Methyltransferase domain
Methyltransf_31 PF13847 124 - 239 7e-15 Methyltransferase domain
Methyltransf_25 PF13649 129 - 223 2.4e-18 Methyltransferase domain
Methyltransf_11 PF08241 130 - 227 2.5e-20 Methyltransferase domain
Methyltransf_12 PF08242 130 - 225 1e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 935
AccB1I GGYRCC 1 cut(s) 693
AccB7I CCANNNNNTGG 1 cut(s) 517
AccII CGCG 3 cut(s) 119, 303, 305
AciI CCGC 6 cut(s) 33, 152, 170, 194, 301, 305
AclWI GGATC 4 cut(s) 237, 711, 976, 1008
AcoI YGGCCR 3 cut(s) 519, 574, 881
AcuI CTGAAG 1 cut(s) 200
AfiI CCNNNNNNNGG 4 cut(s) 304, 315, 517, 879
AgsI TTSAA 3 cut(s) 539, 730, 994
AhdI GACNNNNNGTC 1 cut(s) 211
AjiI CACGTC 1 cut(s) 30
AjnI CCWGG 3 cut(s) 419, 516, 664
AjuI GAANNNNNNNTTGG 2 cut(s) 734, 766
AluBI AGCT 8 cut(s) 21, 139, 178, 417, 646, 748, 785, 814
AluI AGCT 8 cut(s) 21, 139, 178, 417, 646, 748, 785, 814
Alw21I GWGCWC 2 cut(s) 336, 419
Alw26I GTCTC 4 cut(s) 7, 101, 149, 864
AlwI GGATC 4 cut(s) 237, 711, 976, 1008
Ama87I CYCGRG 2 cut(s) 818, 833
AoxI GGCC 6 cut(s) 306, 489, 514, 519, 574, 881
ApeKI GCWGC 4 cut(s) 53, 503, 506, 659
AspLEI GCGC 3 cut(s) 119, 305, 936
AspS9I GGNCC 6 cut(s) 39, 295, 307, 489, 594, 710
AsuC2I CCSGG 1 cut(s) 316
AsuNHI GCTAGC 1 cut(s) 431
AvaI CYCGRG 2 cut(s) 818, 833
AvaII GGWCC 4 cut(s) 39, 295, 594, 710
BalI TGGCCA 2 cut(s) 521, 576
BanI GGYRCC 1 cut(s) 693
BanII GRGCYC 1 cut(s) 419
Bbv12I GWGCWC 2 cut(s) 336, 419
BbvCI CCTCAGC 1 cut(s) 849
BbvI GCAGC 4 cut(s) 65, 490, 493, 646
BccI CCATC 3 cut(s) 157, 566, 721
BceAI ACGGC 2 cut(s) 174, 271
BcgI CGANNNNNNTGC 4 cut(s) 79, 113, 197, 231
BciT130I CCWGG 3 cut(s) 421, 518, 666
BcnI CCSGG 1 cut(s) 316
BcoDI GTCTC 4 cut(s) 7, 101, 149, 864
BfaI CTAG 1 cut(s) 432
BglI GCCNNNNNGGC 1 cut(s) 527
BisI GCNGC 5 cut(s) 54, 306, 504, 507, 660
BlpI GCTNAGC 1 cut(s) 898
BlsI GCNGC 5 cut(s) 55, 307, 505, 508, 661
Bme1390I CCNGG 4 cut(s) 316, 421, 518, 666
Bme18I GGWCC 4 cut(s) 39, 295, 594, 710
BmeRI GACNNNNNGTC 1 cut(s) 211
BmeT110I CYCGRG 2 cut(s) 818, 833
BmgBI CACGTC 1 cut(s) 30
BmgT120I GGNCC 6 cut(s) 39, 295, 307, 489, 594, 710
BmiI GGNNCC 1 cut(s) 695
BmrFI CCNGG 4 cut(s) 316, 421, 518, 666
BmsI GCATC 3 cut(s) 14, 538, 539
BmtI GCTAGC 1 cut(s) 435
BplI GAGNNNNNCTC 2 cut(s) 812, 844
BpmI CTGGAG 2 cut(s) 403, 687
Bpu10I CCTNAGC 1 cut(s) 849
Bpu1102I GCTNAGC 1 cut(s) 898
BpuEI CTTGAG 1 cut(s) 794
BpuMI CCSGG 1 cut(s) 316
BsaI GGTCTC 1 cut(s) 864
BsaJI CCNNGG 5 cut(s) 253, 511, 597, 664, 985
Bsc4I CCNNNNNNNGG 4 cut(s) 304, 315, 517, 879
Bse118I RCCGGY 2 cut(s) 344, 883
Bse1I ACTGG 1 cut(s) 860
Bse3DI GCAATG 2 cut(s) 772, 975
BseBI CCWGG 3 cut(s) 421, 518, 666
BseDI CCNNGG 5 cut(s) 253, 511, 597, 664, 985
BseGI GGATG 2 cut(s) 324, 397
BseLI CCNNNNNNNGG 4 cut(s) 304, 315, 517, 879
BseMI GCAATG 2 cut(s) 772, 975
BseMII CTCAG 5 cut(s) 8, 345, 458, 840, 912
BseNI ACTGG 1 cut(s) 860
BseRI GAGGAG 2 cut(s) 75, 972
BseXI GCAGC 4 cut(s) 65, 490, 493, 646
BsgI GTGCAG 1 cut(s) 497
Bsh1236I CGCG 3 cut(s) 119, 303, 305
BshFI GGCC 6 cut(s) 308, 491, 516, 521, 576, 883
BshNI GGYRCC 1 cut(s) 693
BsiHKAI GWGCWC 2 cut(s) 336, 419
BsiHKCI CYCGRG 2 cut(s) 818, 833
BsiSI CCGG 3 cut(s) 316, 345, 884
BslI CCNNNNNNNGG 4 cut(s) 304, 315, 517, 879
BsmAI GTCTC 4 cut(s) 7, 101, 149, 864
BsmBI CGTCTC 1 cut(s) 149
BsnI GGCC 6 cut(s) 308, 491, 516, 521, 576, 883
Bso31I GGTCTC 1 cut(s) 864
BsoBI CYCGRG 2 cut(s) 818, 833
Bsp1286I GDGCHC 2 cut(s) 336, 419
Bsp143I GATC 6 cut(s) 229, 321, 583, 703, 981, 1000
Bsp1720I GCTNAGC 1 cut(s) 898
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 6 cut(s) 33, 152, 170, 194, 301, 305
BspANI GGCC 6 cut(s) 308, 491, 516, 521, 576, 883
BspCNI CTCAG 5 cut(s) 9, 346, 459, 841, 911
BspFNI CGCG 3 cut(s) 119, 303, 305
BspLI GGNNCC 1 cut(s) 695
BspOI GCTAGC 1 cut(s) 435
BspPI GGATC 4 cut(s) 237, 711, 976, 1008
BspT107I GGYRCC 1 cut(s) 693
BspTNI GGTCTC 1 cut(s) 864
BsrDI GCAATG 2 cut(s) 772, 975
BsrFI RCCGGY 2 cut(s) 344, 883
BsrI ACTGG 1 cut(s) 860
BssAI RCCGGY 2 cut(s) 344, 883
BssECI CCNNGG 5 cut(s) 253, 511, 597, 664, 985
BssMI GATC 6 cut(s) 229, 321, 583, 703, 981, 1000
BssT1I CCWWGG 3 cut(s) 511, 597, 985
Bst2UI CCWGG 3 cut(s) 421, 518, 666
Bst4CI ACNGT 1 cut(s) 723
Bst6I CTCTTC 1 cut(s) 119
BstC8I GCNNGC 5 cut(s) 115, 346, 433, 480, 885
BstDEI CTNAG 6 cut(s) 17, 140, 354, 467, 849, 898
BstDSI CCRYGG 2 cut(s) 253, 597
BstF5I GGATG 2 cut(s) 324, 397
BstFNI CGCG 3 cut(s) 119, 303, 305
BstHHI GCGC 3 cut(s) 119, 305, 936
BstKTI GATC 6 cut(s) 232, 324, 586, 706, 984, 1003
BstMAI GTCTC 4 cut(s) 7, 101, 149, 864
BstMBI GATC 6 cut(s) 229, 321, 583, 703, 981, 1000
BstMWI GCNNNNNNNGC 6 cut(s) 158, 167, 488, 503, 527, 668
BstNI CCWGG 3 cut(s) 421, 518, 666
BstNSI RCATGY 2 cut(s) 619, 1029
BstSCI CCNGG 4 cut(s) 314, 419, 516, 664
BstUI CGCG 3 cut(s) 119, 303, 305
BstV1I GCAGC 4 cut(s) 65, 490, 493, 646
BstX2I RGATCY 3 cut(s) 229, 703, 1000
BstXI CCANNNNNNTGG 1 cut(s) 427
BstYI RGATCY 3 cut(s) 229, 703, 1000
BsuRI GGCC 6 cut(s) 308, 491, 516, 521, 576, 883
BtgI CCRYGG 2 cut(s) 253, 597
BtgZI GCGATG 1 cut(s) 176
BtrI CACGTC 1 cut(s) 30
BtsCI GGATG 2 cut(s) 324, 397
BtsI GCAGTG 1 cut(s) 894
BtsIMutI CAGTG 2 cut(s) 894, 974
Cac8I GCNNGC 5 cut(s) 115, 346, 433, 480, 885
CfoI GCGC 3 cut(s) 119, 305, 936
Cfr10I RCCGGY 2 cut(s) 344, 883
Cfr13I GGNCC 6 cut(s) 39, 295, 307, 489, 594, 710
CviAII CATG 4 cut(s) 598, 616, 690, 1026
DdeI CTNAG 6 cut(s) 17, 140, 354, 467, 849, 898
DpnI GATC 6 cut(s) 231, 323, 585, 705, 983, 1002
DpnII GATC 6 cut(s) 229, 321, 583, 703, 981, 1000
DriI GACNNNNNGTC 1 cut(s) 211
EaeI YGGCCR 3 cut(s) 519, 574, 881
Eam1104I CTCTTC 1 cut(s) 119
Eam1105I GACNNNNNGTC 1 cut(s) 211
EarI CTCTTC 1 cut(s) 119
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 417
Eco130I CCWWGG 3 cut(s) 511, 597, 985
Eco147I AGGCCT 1 cut(s) 516
Eco24I GRGCYC 1 cut(s) 419
Eco31I GGTCTC 1 cut(s) 864
Eco32I GATATC 1 cut(s) 841
Eco47I GGWCC 4 cut(s) 39, 295, 594, 710
Eco53kI GAGCTC 1 cut(s) 417
Eco57I CTGAAG 1 cut(s) 200
Eco88I CYCGRG 2 cut(s) 818, 833
EcoICRI GAGCTC 1 cut(s) 417
EcoRII CCWGG 3 cut(s) 419, 516, 664
EcoRV GATATC 1 cut(s) 841
EcoT14I CCWWGG 3 cut(s) 511, 597, 985
EcoT38I GRGCYC 1 cut(s) 419
ErhI CCWWGG 3 cut(s) 511, 597, 985
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 4 cut(s) 601, 619, 693, 1029
FalI AAGNNNNNCTT 4 cut(s) 734, 766, 905, 937
FatI CATG 4 cut(s) 597, 615, 689, 1025
FauI CCCGC 1 cut(s) 26
FauNDI CATATG 2 cut(s) 246, 865
Fnu4HI GCNGC 5 cut(s) 54, 306, 504, 507, 660
FokI GGATG 2 cut(s) 331, 404
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 5 cut(s) 54, 306, 504, 507, 660
FspBI CTAG 1 cut(s) 432
FspI TGCGCA 1 cut(s) 935
GlaI GCGC 3 cut(s) 118, 304, 935
GluI GCNGC 5 cut(s) 54, 306, 504, 507, 660
GsuI CTGGAG 2 cut(s) 403, 687
HaeIII GGCC 6 cut(s) 308, 491, 516, 521, 576, 883
HapII CCGG 3 cut(s) 316, 345, 884
HhaI GCGC 3 cut(s) 119, 305, 936
Hin1II CATG 4 cut(s) 601, 619, 693, 1029
Hin6I GCGC 3 cut(s) 117, 303, 934
HinP1I GCGC 3 cut(s) 117, 303, 934
HinfI GANTC 6 cut(s) 209, 339, 469, 821, 892, 990
HpaII CCGG 3 cut(s) 316, 345, 884
Hpy188I TCNGA 5 cut(s) 295, 338, 355, 468, 718
Hpy188III TCNNGA 2 cut(s) 569, 994
Hpy99I CGWCG 5 cut(s) 13, 49, 52, 209, 329
HpyAV CCTTC 4 cut(s) 89, 178, 367, 991
HpyCH4III ACNGT 1 cut(s) 723
HpyCH4IV ACGT 2 cut(s) 29, 282
HpyCH4V TGCA 5 cut(s) 250, 453, 478, 557, 765
HpyF10VI GCNNNNNNNGC 6 cut(s) 158, 167, 488, 503, 527, 668
HpyF3I CTNAG 6 cut(s) 17, 140, 354, 467, 849, 898
HpySE526I ACGT 2 cut(s) 29, 282
Hsp92II CATG 4 cut(s) 601, 619, 693, 1029
HspAI GCGC 3 cut(s) 117, 303, 934
KroI GCCGGC 2 cut(s) 344, 883
KroNI GCCGGC 2 cut(s) 346, 885
Kzo9I GATC 6 cut(s) 229, 321, 583, 703, 981, 1000
LmnI GCTCC 7 cut(s) 175, 339, 414, 422, 443, 668, 959
Lsp1109I GCAGC 4 cut(s) 65, 490, 493, 646
LweI GCATC 3 cut(s) 14, 538, 539
MaeI CTAG 1 cut(s) 432
MaeII ACGT 2 cut(s) 29, 282
MaeIII GTNAC 1 cut(s) 685
MalI GATC 6 cut(s) 231, 323, 585, 705, 983, 1002
MboI GATC 6 cut(s) 229, 321, 583, 703, 981, 1000
MboII GAAGA 6 cut(s) 133, 136, 193, 391, 754, 770
MflI RGATCY 3 cut(s) 229, 703, 1000
MhlI GDGCHC 2 cut(s) 336, 419
MlsI TGGCCA 2 cut(s) 521, 576
MluCI AATT 2 cut(s) 448, 578
MluNI TGGCCA 2 cut(s) 521, 576
MlyI GAGTC 3 cut(s) 218, 478, 830
Mox20I TGGCCA 2 cut(s) 521, 576
MroNI GCCGGC 2 cut(s) 344, 883
MscI TGGCCA 2 cut(s) 521, 576
MseI TTAA 1 cut(s) 912
Msp20I TGGCCA 2 cut(s) 521, 576
MspI CCGG 3 cut(s) 316, 345, 884
MspR9I CCNGG 4 cut(s) 316, 421, 518, 666
MvaI CCWGG 3 cut(s) 421, 518, 666
MvnI CGCG 3 cut(s) 119, 303, 305
MwoI GCNNNNNNNGC 6 cut(s) 158, 167, 488, 503, 527, 668
NaeI GCCGGC 2 cut(s) 346, 885
NciI CCSGG 1 cut(s) 316
NcoI CCATGG 1 cut(s) 597
NdeI CATATG 2 cut(s) 246, 865
NdeII GATC 6 cut(s) 229, 321, 583, 703, 981, 1000
NgoMIV GCCGGC 2 cut(s) 344, 883
NheI GCTAGC 1 cut(s) 431
NlaIII CATG 4 cut(s) 601, 619, 693, 1029
NlaIV GGNNCC 1 cut(s) 695
NsbI TGCGCA 1 cut(s) 935
NspI RCATGY 2 cut(s) 619, 1029
PaeR7I CTCGAG 2 cut(s) 818, 833
PceI AGGCCT 1 cut(s) 516
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PdiI GCCGGC 2 cut(s) 346, 885
PfeI GAWTC 3 cut(s) 339, 892, 990
PflMI CCANNNNNTGG 1 cut(s) 517
PkrI GCNGC 5 cut(s) 55, 307, 505, 508, 661
PleI GAGTC 3 cut(s) 217, 477, 829
PpsI GAGTC 3 cut(s) 217, 477, 829
Psp124BI GAGCTC 1 cut(s) 419
Psp6I CCWGG 3 cut(s) 419, 516, 664
PspGI CCWGG 3 cut(s) 419, 516, 664
PspN4I GGNNCC 1 cut(s) 695
PspPI GGNCC 6 cut(s) 39, 295, 307, 489, 594, 710
PspXI VCTCGAGB 2 cut(s) 818, 833
PsrI GAACNNNNNNTAC 2 cut(s) 52, 84
PsuI RGATCY 3 cut(s) 229, 703, 1000
SacI GAGCTC 1 cut(s) 419
SaqAI TTAA 1 cut(s) 912
SatI GCNGC 5 cut(s) 54, 306, 504, 507, 660
Sau3AI GATC 6 cut(s) 229, 321, 583, 703, 981, 1000
Sau96I GGNCC 6 cut(s) 39, 295, 307, 489, 594, 710
SchI GAGTC 3 cut(s) 218, 478, 830
ScrFI CCNGG 4 cut(s) 316, 421, 518, 666
SduI GDGCHC 2 cut(s) 336, 419
SfaNI GCATC 3 cut(s) 14, 538, 539
Sfr274I CTCGAG 2 cut(s) 818, 833
SinI GGWCC 4 cut(s) 39, 295, 594, 710
SlaI CTCGAG 2 cut(s) 818, 833
SmlI CTYRAG 3 cut(s) 809, 818, 833
SmoI CTYRAG 3 cut(s) 809, 818, 833
Sse9I AATT 2 cut(s) 448, 578
SseBI AGGCCT 1 cut(s) 516
SsiI CCGC 6 cut(s) 33, 152, 170, 194, 301, 305
SspI AATATT 1 cut(s) 1013
SspMI CTAG 1 cut(s) 432
SstI GAGCTC 1 cut(s) 419
StuI AGGCCT 1 cut(s) 516
StyD4I CCNGG 4 cut(s) 314, 419, 516, 664
StyI CCWWGG 3 cut(s) 511, 597, 985
TaaI ACNGT 1 cut(s) 723
TaiI ACGT 2 cut(s) 32, 285
TaqI TCGA 5 cut(s) 8, 324, 652, 819, 834
TasI AATT 2 cut(s) 448, 578
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 3 cut(s) 339, 892, 990
Tru1I TTAA 1 cut(s) 912
Tru9I TTAA 1 cut(s) 912
TscAI CASTG 2 cut(s) 894, 981
TseI GCWGC 4 cut(s) 53, 503, 506, 659
TspRI CASTG 2 cut(s) 894, 981
Van91I CCANNNNNTGG 1 cut(s) 517
VpaK11BI GGWCC 4 cut(s) 39, 295, 594, 710
XceI RCATGY 2 cut(s) 619, 1029
XcmI CCANNNNNNNNNTGG 1 cut(s) 704
XhoI CTCGAG 2 cut(s) 818, 833
XspI CTAG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.