Rh3DG175400
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. gTMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0011326
Physical Location & Seq
Forward (+)
15566444 .. 15569956
3513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG175400.1

Sequence Viewer

Length: 960 bp
ATGGCATCTACGAGGCTGAACTACCAGACGTGCAGGTGGTCCTCGCCAGCAGCAACCACGCTTCCTCCTCGCACGTGTCTGGGTGTGGCTGGTGTGAACGGAGCAGCGGTGAGCTCGAAGAAGAAGAAGAAGAGCGGGTTTGAGTTGAGAGCAGTGGCGGCGACGGCGATGGAGGCAGTGGAGCTGAAGAAGGACATAGCCGAGTTCTACGACGAGTCGTCTGGGTTATGGGAGGACCTCTGGGGCGACCACATGCACCACGGCTTCTACGACCCCAAGGCCGCCGACGTTTCGGTGTCGGACCACCGGGCGGCCCAGACCCGGATGATCGACGAGGTGCTACGGTTCGCAGGGATTTCTGACAAGTGTCCAATTGGGAATGTAGTGGATGTTGGGTGTGGGATTGGAGGGAGCTCCAGATATCTGGCCAGCAAATACGGGGCCAATTGCAAAGGGATTACTCTGAGCCCCGTACAGGCTCTGAGGGCCAATGCTCTTGCTGCTGCTCAAGGCCTAGCCAATAAGGCATCCTTTCAAGTTGCTGATGCTCTAGCACAACCATTTCCTGATGGCCAATTTGATCTGGTTTGGTCCATGGAGAGTGGGGAACACATGCCTGATAAAGCAAAGTTTGTTGATGAGTTGGTTCGAGTTGCGGCACCAGGAGCAACGATAATACTAGTTACATGGTGCCACAGGGATCTTGGCGCTTCAGAACAATCATTGCAGCCAGATGAGAAAAAGCTCCTGGACAAGATATGCAATGCTTTCTATCTTCCTGCTTGGTGTTCTACTGCTGACTACCTCAAGCTACTCGAGTCCTACTCACTCAAGGATATCAAGGCTGAGGACTGGTCTCCGTATGTTGCCCCATTTTGGCCGGCAGTGATTCGCTCAGCATTGACATTTAAGGGTTTCTTTTCTCTGTTGCGCACAGGTAAACTTAGCACTTTTTTCTAG

Protein Analysis

319

Amino Acids

34.73

Weight (kDa)

6.99

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 64 - 227 4.3e-11 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 104 - 242 9.1e-13 Methyltransferase domain
Methyltransf_31 PF13847 127 - 238 2.1e-12 Methyltransferase domain
Methyltransf_25 PF13649 128 - 222 1.4e-17 Methyltransferase domain
Methyltransf_11 PF08241 129 - 226 1.6e-19 Methyltransferase domain
Methyltransf_12 PF08242 129 - 224 2.1e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 24
Acc16I TGCGCA 1 cut(s) 932
Acc36I ACCTGC 1 cut(s) 24
AccB1I GGYRCC 2 cut(s) 658, 690
AccBSI CCGCTC 1 cut(s) 135
AciI CCGC 6 cut(s) 107, 135, 158, 282, 311, 656
AclWI GGATC 1 cut(s) 708
AcoI YGGCCR 3 cut(s) 426, 571, 878
AcuI CTGAAG 2 cut(s) 206, 696
AcvI CACGTG 1 cut(s) 75
AfaI GTAC 1 cut(s) 474
AfiI CCNNNNNNNGG 4 cut(s) 310, 321, 475, 876
AflIII ACRYGT 1 cut(s) 74
AgsI TTSAA 1 cut(s) 536
AhdI GACNNNNNGTC 1 cut(s) 217
AhlI ACTAGT 1 cut(s) 679
AjiI CACGTC 1 cut(s) 30
AjnI CCWGG 2 cut(s) 661, 747
AluBI AGCT 5 cut(s) 114, 184, 414, 745, 811
AluI AGCT 5 cut(s) 114, 184, 414, 745, 811
Alw21I GWGCWC 2 cut(s) 116, 416
Alw26I GTCTC 1 cut(s) 861
AlwI GGATC 1 cut(s) 708
AlwNI CAGNNNCTG 1 cut(s) 481
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 8 cut(s) 279, 312, 426, 441, 486, 511, 571, 878
ApeKI GCWGC 5 cut(s) 50, 104, 500, 503, 727
AspLEI GCGC 2 cut(s) 710, 933
AspS9I GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
AsuC2I CCSGG 2 cut(s) 308, 322
AsuHPI GGTGA 1 cut(s) 121
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 4 cut(s) 39, 235, 301, 591
BalI TGGCCA 2 cut(s) 428, 573
BanI GGYRCC 2 cut(s) 658, 690
BanII GRGCYC 3 cut(s) 116, 416, 470
BbrPI CACGTG 1 cut(s) 75
Bbv12I GWGCWC 2 cut(s) 116, 416
BbvCI CCTCAGC 1 cut(s) 846
BbvI GCAGC 5 cut(s) 62, 116, 487, 490, 739
BccI CCATC 2 cut(s) 163, 563
BceAI ACGGC 2 cut(s) 180, 277
BciT130I CCWGG 2 cut(s) 663, 749
BcnI CCSGG 2 cut(s) 308, 322
BcoDI GTCTC 1 cut(s) 861
BcuI ACTAGT 1 cut(s) 679
BfaI CTAG 4 cut(s) 515, 551, 680, 958
BfoI RGCGCY 1 cut(s) 711
BfuAI ACCTGC 1 cut(s) 24
BglI GCCNNNNNGGC 1 cut(s) 524
BisI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 501, 504, 657, 728
BlpI GCTNAGC 1 cut(s) 895
BlsI GCNGC 9 cut(s) 52, 106, 160, 283, 313, 502, 505, 658, 729
Bme1390I CCNGG 4 cut(s) 308, 322, 663, 749
Bme18I GGWCC 4 cut(s) 39, 235, 301, 591
BmeRI GACNNNNNGTC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 815
BmgBI CACGTC 1 cut(s) 30
BmgT120I GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
BmiI GGNNCC 3 cut(s) 442, 660, 692
BmrFI CCNGG 4 cut(s) 308, 322, 663, 749
BmsI GCATC 3 cut(s) 14, 535, 536
BoxI GACNNNNGTC 1 cut(s) 366
BplI GAGNNNNNCTC 2 cut(s) 809, 841
BpmI CTGGAG 1 cut(s) 400
Bpu10I CCTNAGC 1 cut(s) 846
Bpu1102I GCTNAGC 1 cut(s) 895
BpuEI CTTGAG 3 cut(s) 492, 791, 815
BpuMI CCSGG 2 cut(s) 308, 322
BsaAI YACGTR 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 861
BsaJI CCNNGG 3 cut(s) 259, 276, 594
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 93, 123
Bsc4I CCNNNNNNNGG 4 cut(s) 310, 321, 475, 876
Bse118I RCCGGY 1 cut(s) 880
Bse1I ACTGG 1 cut(s) 857
Bse3DI GCAATG 2 cut(s) 722, 769
BseBI CCWGG 2 cut(s) 663, 749
BseDI CCNNGG 3 cut(s) 259, 276, 594
BseGI GGATG 3 cut(s) 330, 394, 527
BseLI CCNNNNNNNGG 4 cut(s) 310, 321, 475, 876
BseMI GCAATG 2 cut(s) 722, 769
BseMII CTCAG 4 cut(s) 455, 473, 837, 909
BseNI ACTGG 1 cut(s) 857
BseRI GAGGAG 1 cut(s) 57
BseXI GCAGC 5 cut(s) 62, 116, 487, 490, 739
BsgI GTGCAG 1 cut(s) 52
BshFI GGCC 8 cut(s) 281, 314, 428, 443, 488, 513, 573, 880
BshNI GGYRCC 2 cut(s) 658, 690
BsiHKAI GWGCWC 2 cut(s) 116, 416
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 3 cut(s) 307, 322, 881
BslI CCNNNNNNNGG 4 cut(s) 310, 321, 475, 876
BsmAI GTCTC 1 cut(s) 861
BsnI GGCC 8 cut(s) 281, 314, 428, 443, 488, 513, 573, 880
Bso31I GGTCTC 1 cut(s) 861
BsoBI CYCGRG 1 cut(s) 815
Bsp1286I GDGCHC 3 cut(s) 116, 416, 470
Bsp143I GATC 3 cut(s) 327, 580, 700
Bsp1720I GCTNAGC 1 cut(s) 895
Bsp19I CCATGG 1 cut(s) 594
BspACI CCGC 6 cut(s) 107, 135, 158, 282, 311, 656
BspANI GGCC 8 cut(s) 281, 314, 428, 443, 488, 513, 573, 880
BspCNI CTCAG 4 cut(s) 456, 474, 838, 908
BspLI GGNNCC 3 cut(s) 442, 660, 692
BspMI ACCTGC 1 cut(s) 24
BspPI GGATC 1 cut(s) 708
BspQI GCTCTTC 1 cut(s) 125
BspT107I GGYRCC 2 cut(s) 658, 690
BspTNI GGTCTC 1 cut(s) 861
BsrBI CCGCTC 1 cut(s) 135
BsrDI GCAATG 2 cut(s) 722, 769
BsrFI RCCGGY 1 cut(s) 880
BsrI ACTGG 1 cut(s) 857
BssAI RCCGGY 1 cut(s) 880
BssECI CCNNGG 3 cut(s) 259, 276, 594
BssMI GATC 3 cut(s) 327, 580, 700
BssT1I CCWWGG 2 cut(s) 276, 594
Bst2UI CCWGG 2 cut(s) 663, 749
Bst4CI ACNGT 1 cut(s) 345
Bst6I CTCTTC 1 cut(s) 125
BstBAI YACGTR 1 cut(s) 75
BstC8I GCNNGC 3 cut(s) 48, 430, 882
BstDEI CTNAG 5 cut(s) 464, 482, 846, 895, 944
BstDSI CCRYGG 2 cut(s) 259, 594
BstF5I GGATG 3 cut(s) 330, 394, 527
BstH2I RGCGCY 1 cut(s) 711
BstHHI GCGC 2 cut(s) 710, 933
BstKTI GATC 3 cut(s) 330, 583, 703
BstMAI GTCTC 1 cut(s) 861
BstMBI GATC 3 cut(s) 327, 580, 700
BstMWI GCNNNNNNNGC 7 cut(s) 158, 164, 173, 485, 500, 524, 665
BstNI CCWGG 2 cut(s) 663, 749
BstNSI RCATGY 2 cut(s) 256, 616
BstPAI GACNNNNGTC 1 cut(s) 366
BstSCI CCNGG 4 cut(s) 306, 320, 661, 747
BstV1I GCAGC 5 cut(s) 62, 116, 487, 490, 739
BstX2I RGATCY 1 cut(s) 700
BstXI CCANNNNNNTGG 1 cut(s) 424
BstYI RGATCY 1 cut(s) 700
BsuRI GGCC 8 cut(s) 281, 314, 428, 443, 488, 513, 573, 880
BtgI CCRYGG 2 cut(s) 259, 594
BtgZI GCGATG 1 cut(s) 182
BtrI CACGTC 1 cut(s) 30
BtsCI GGATG 3 cut(s) 330, 394, 527
BtsI GCAGTG 3 cut(s) 159, 183, 891
BtsIMutI CAGTG 3 cut(s) 159, 183, 891
BveI ACCTGC 1 cut(s) 24
Cac8I GCNNGC 3 cut(s) 48, 430, 882
CaiI CAGNNNCTG 1 cut(s) 481
CfoI GCGC 2 cut(s) 710, 933
Cfr10I RCCGGY 1 cut(s) 880
Cfr13I GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
Csp6I GTAC 1 cut(s) 473
CviAII CATG 4 cut(s) 253, 595, 613, 687
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 5 cut(s) 464, 482, 846, 895, 944
DpnI GATC 3 cut(s) 329, 582, 702
DpnII GATC 3 cut(s) 327, 580, 700
DriI GACNNNNNGTC 1 cut(s) 217
EaeI YGGCCR 3 cut(s) 426, 571, 878
Eam1104I CTCTTC 1 cut(s) 125
Eam1105I GACNNNNNGTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 125
Ecl136II GAGCTC 2 cut(s) 114, 414
Eco130I CCWWGG 2 cut(s) 276, 594
Eco147I AGGCCT 1 cut(s) 513
Eco24I GRGCYC 3 cut(s) 116, 416, 470
Eco31I GGTCTC 1 cut(s) 861
Eco32I GATATC 2 cut(s) 422, 838
Eco47I GGWCC 4 cut(s) 39, 235, 301, 591
Eco53kI GAGCTC 2 cut(s) 114, 414
Eco57I CTGAAG 2 cut(s) 206, 696
Eco72I CACGTG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 815
EcoICRI GAGCTC 2 cut(s) 114, 414
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 2 cut(s) 661, 747
EcoRV GATATC 2 cut(s) 422, 838
EcoT14I CCWWGG 2 cut(s) 276, 594
EcoT38I GRGCYC 3 cut(s) 116, 416, 470
ErhI CCWWGG 2 cut(s) 276, 594
FaeI CATG 4 cut(s) 256, 598, 616, 690
FaiI YATR 8 cut(s) 197, 229, 254, 596, 614, 688, 760, 864
FalI AAGNNNNNCTT 4 cut(s) 515, 547, 902, 934
FatI CATG 4 cut(s) 252, 594, 612, 686
FauI CCCGC 1 cut(s) 128
Fnu4HI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 501, 504, 657, 728
FokI GGATG 3 cut(s) 337, 401, 514
FriOI GRGCYC 3 cut(s) 116, 416, 470
Fsp4HI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 501, 504, 657, 728
FspBI CTAG 4 cut(s) 515, 551, 680, 958
FspI TGCGCA 1 cut(s) 932
GlaI GCGC 2 cut(s) 709, 932
GluI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 501, 504, 657, 728
GsuI CTGGAG 1 cut(s) 400
HaeII RGCGCY 1 cut(s) 711
HaeIII GGCC 8 cut(s) 281, 314, 428, 443, 488, 513, 573, 880
HapII CCGG 3 cut(s) 307, 322, 881
HhaI GCGC 2 cut(s) 710, 933
Hin1II CATG 4 cut(s) 256, 598, 616, 690
Hin6I GCGC 2 cut(s) 708, 931
HinP1I GCGC 2 cut(s) 708, 931
HinfI GANTC 3 cut(s) 215, 818, 889
HpaII CCGG 3 cut(s) 307, 322, 881
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 2 cut(s) 97, 941
Hpy188I TCNGA 5 cut(s) 301, 361, 465, 483, 715
Hpy188III TCNNGA 2 cut(s) 417, 566
Hpy8I GTNNAC 2 cut(s) 97, 941
Hpy99I CGWCG 4 cut(s) 166, 215, 290, 335
HpyAV CCTTC 1 cut(s) 184
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4IV ACGT 3 cut(s) 29, 74, 288
HpyCH4V TGCA 5 cut(s) 33, 256, 450, 727, 762
HpyF10VI GCNNNNNNNGC 7 cut(s) 158, 164, 173, 485, 500, 524, 665
HpyF3I CTNAG 5 cut(s) 464, 482, 846, 895, 944
HpySE526I ACGT 3 cut(s) 29, 74, 288
Hsp92II CATG 4 cut(s) 256, 598, 616, 690
HspAI GCGC 2 cut(s) 708, 931
KroI GCCGGC 1 cut(s) 880
KroNI GCCGGC 1 cut(s) 882
Kzo9I GATC 3 cut(s) 327, 580, 700
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 6 cut(s) 101, 181, 411, 419, 665, 750
Lsp1109I GCAGC 5 cut(s) 62, 116, 487, 490, 739
LweI GCATC 3 cut(s) 14, 535, 536
MaeI CTAG 4 cut(s) 515, 551, 680, 958
MaeII ACGT 3 cut(s) 29, 74, 288
MaeIII GTNAC 1 cut(s) 682
MalI GATC 3 cut(s) 329, 582, 702
MbiI CCGCTC 1 cut(s) 135
MboI GATC 3 cut(s) 327, 580, 700
MboII GAAGA 7 cut(s) 130, 133, 136, 139, 142, 199, 767
MfeI CAATTG 2 cut(s) 372, 445
MflI RGATCY 1 cut(s) 700
MhlI GDGCHC 3 cut(s) 116, 416, 470
MlsI TGGCCA 2 cut(s) 428, 573
MluCI AATT 3 cut(s) 372, 445, 575
MluNI TGGCCA 2 cut(s) 428, 573
MlyI GAGTC 2 cut(s) 224, 827
MmeI TCCRAC 1 cut(s) 279
Mox20I TGGCCA 2 cut(s) 428, 573
MroNI GCCGGC 1 cut(s) 880
MscI TGGCCA 2 cut(s) 428, 573
MseI TTAA 1 cut(s) 909
Msp20I TGGCCA 2 cut(s) 428, 573
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 3 cut(s) 307, 322, 881
MspR9I CCNGG 4 cut(s) 308, 322, 663, 749
MunI CAATTG 2 cut(s) 372, 445
MvaI CCWGG 2 cut(s) 663, 749
MwoI GCNNNNNNNGC 7 cut(s) 158, 164, 173, 485, 500, 524, 665
NaeI GCCGGC 1 cut(s) 882
NciI CCSGG 2 cut(s) 308, 322
NcoI CCATGG 1 cut(s) 594
NdeII GATC 3 cut(s) 327, 580, 700
NgoMIV GCCGGC 1 cut(s) 880
NlaIII CATG 4 cut(s) 256, 598, 616, 690
NlaIV GGNNCC 3 cut(s) 442, 660, 692
NmeAIII GCCGAG 1 cut(s) 226
NsbI TGCGCA 1 cut(s) 932
NspI RCATGY 2 cut(s) 256, 616
PaeR7I CTCGAG 1 cut(s) 815
PaqCI CACCTGC 1 cut(s) 24
PceI AGGCCT 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 125
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PdiI GCCGGC 1 cut(s) 882
PfeI GAWTC 1 cut(s) 889
PfoI TCCNGGA 1 cut(s) 747
PkrI GCNGC 9 cut(s) 52, 106, 160, 283, 313, 502, 505, 658, 729
PleI GAGTC 2 cut(s) 223, 826
PmaCI CACGTG 1 cut(s) 75
PmlI CACGTG 1 cut(s) 75
PpsI GAGTC 2 cut(s) 223, 826
Ppu21I YACGTR 1 cut(s) 75
PpuMI RGGWCCY 1 cut(s) 235
PshAI GACNNNNGTC 1 cut(s) 366
Psp124BI GAGCTC 2 cut(s) 116, 416
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 2 cut(s) 661, 747
PspCI CACGTG 1 cut(s) 75
PspGI CCWGG 2 cut(s) 661, 747
PspN4I GGNNCC 3 cut(s) 442, 660, 692
PspPI GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
PspPPI RGGWCCY 1 cut(s) 235
PspXI VCTCGAGB 1 cut(s) 815
PstNI CAGNNNCTG 1 cut(s) 481
PsuI RGATCY 1 cut(s) 700
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
SacI GAGCTC 2 cut(s) 116, 416
SapI GCTCTTC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 909
SatI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 501, 504, 657, 728
Sau3AI GATC 3 cut(s) 327, 580, 700
Sau96I GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
SchI GAGTC 2 cut(s) 224, 827
ScrFI CCNGG 4 cut(s) 308, 322, 663, 749
SduI GDGCHC 3 cut(s) 116, 416, 470
SfaNI GCATC 3 cut(s) 14, 535, 536
Sfr274I CTCGAG 1 cut(s) 815
SinI GGWCC 4 cut(s) 39, 235, 301, 591
SlaI CTCGAG 1 cut(s) 815
SmlI CTYRAG 4 cut(s) 507, 806, 815, 830
SmoI CTYRAG 4 cut(s) 507, 806, 815, 830
SpeI ACTAGT 1 cut(s) 679
Sse9I AATT 3 cut(s) 372, 445, 575
SseBI AGGCCT 1 cut(s) 513
SsiI CCGC 6 cut(s) 107, 135, 158, 282, 311, 656
SspMI CTAG 4 cut(s) 515, 551, 680, 958
SstI GAGCTC 2 cut(s) 116, 416
StuI AGGCCT 1 cut(s) 513
StyD4I CCNGG 4 cut(s) 306, 320, 661, 747
StyI CCWWGG 2 cut(s) 276, 594
TaaI ACNGT 1 cut(s) 345
TaiI ACGT 3 cut(s) 32, 77, 291
TaqI TCGA 4 cut(s) 116, 330, 649, 816
TasI AATT 3 cut(s) 372, 445, 575
TauI GCSGC 4 cut(s) 161, 284, 314, 659
TfiI GAWTC 1 cut(s) 889
Tru1I TTAA 1 cut(s) 909
Tru9I TTAA 1 cut(s) 909
TscAI CASTG 3 cut(s) 159, 183, 891
TseI GCWGC 5 cut(s) 50, 104, 500, 503, 727
TspGWI ACGGA 2 cut(s) 114, 849
TspRI CASTG 3 cut(s) 159, 183, 891
VpaK11BI GGWCC 4 cut(s) 39, 235, 301, 591
XceI RCATGY 2 cut(s) 256, 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 701
XhoI CTCGAG 1 cut(s) 815
XspI CTAG 4 cut(s) 515, 551, 680, 958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.