RLG00000024108
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. gTMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011326
Physical Location & Seq
Forward (+)
31976694 .. 31982536
5843 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024108

Sequence Viewer

Length: 1050 bp
ATGGCATCTACGGGGCTGAGCTACCAGACGTGCGGGTGGTCCTCGCCAGCAGCAACTACGCTTCCTCCTCGCACGTGTCTGGGTGTAGCTCGTGTGAACGGAGCAGCAGTGAGCTCGAAGAAGAAGAAGAAGAGAGGGTTTGAGTTGAGAGCAGTGGCGGCGACGGCGATGGAGGCAGTGGAGCTGAAGAAGGACATAGCCGAGTTCTACGACGAGTCGTCTGGGTTATGGGAGGATCTCTGGGGCGACCACATGCACCACGGCTTCTACGACCCCAAGGCTGCCGACGTTTCGGTGTCGGACCACCGGGCGGCCCAGACCCGGATGGTCGACGAGGTGCTCCGGTTCGCAGGGATTTCTGAGATGTGTCCAATTGGGAAGGGGAAGAATGTAGTGGATGTTGGGTGTGGGATTGGAGGGAGCTCCAGATATCTGGCTAGCAAATACGGGGCCAATTGCAAAGGGATTACTCTGAGTCCCGTACAGGCTCAAAGGGCCAATGCTCTTGCTGCTGCTCAAGGCCTAGCCAATAAGGCATCCTTTCAAGTTGGTGATGCTCTAGCACAACCATTTCCTGATGGCCAATTTGATCTGGTTTGGTCCATGGAGAGTGGGGAACACATGCCTGATAAAGCAAAGTTTGTTGATGAGTTGGTTCGAGTTGCTGCCCCAGGAGCAACGATAATACTAGTTACATGGTGCCACAGGGATCTTGGTGCTTCTGAACAATCCTTGCAGCCAGATGAGAAAAAGCTCCTGGACAAGATATGCAATGCTTTCTATCTTCCAGCTTGGTGTTCTACTGCTGACTACCTCAAGCTACTCGAGTCCTACTCACTCAAGGATATCAAGGCTGAGGACTGGTCTCCGTATGTTGCCCCATTTTGGCCGGCAGTGATTCGCTCAGCATTGACATTTAAGGGTTTCTTTTCTCTGTTGCGCACAGGAACGAAAACAATAAGAGGAGCATTGGCAATGCCATTGATGATCCAAGGATTCAAGAAGGATCTAATTAAGTATTCCGTTATTACATGCCGAAAACCTGAATAG

Protein Analysis

350

Amino Acids

38.0

Weight (kDa)

8.61

Isoelectric Point (pI)

35.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 64 - 230 3.6e-12 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 104 - 245 1.3e-13 Methyltransferase domain
Methyltransf_31 PF13847 126 - 241 3.6e-15 Methyltransferase domain
Methyltransf_25 PF13649 131 - 225 2.1e-18 Methyltransferase domain
Methyltransf_11 PF08241 132 - 229 2.4e-20 Methyltransferase domain
Methyltransf_12 PF08242 132 - 227 1.2e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 941
AccB1I GGYRCC 1 cut(s) 699
AccI GTMKAC 1 cut(s) 330
AciI CCGC 3 cut(s) 33, 158, 311
AclWI GGATC 4 cut(s) 243, 717, 982, 1014
AcoI YGGCCR 2 cut(s) 580, 887
AcuI CTGAAG 1 cut(s) 206
AcvI CACGTG 1 cut(s) 75
AfaI GTAC 1 cut(s) 483
AfiI CCNNNNNNNGG 3 cut(s) 310, 321, 885
AflIII ACRYGT 1 cut(s) 74
AgsI TTSAA 2 cut(s) 545, 1000
AhdI GACNNNNNGTC 1 cut(s) 217
AhlI ACTAGT 1 cut(s) 688
AjiI CACGTC 1 cut(s) 30
AjnI CCWGG 2 cut(s) 670, 756
AluBI AGCT 8 cut(s) 21, 89, 114, 184, 423, 754, 791, 820
AluI AGCT 8 cut(s) 21, 89, 114, 184, 423, 754, 791, 820
Alw21I GWGCWC 3 cut(s) 116, 342, 425
Alw26I GTCTC 1 cut(s) 870
AlwI GGATC 4 cut(s) 243, 717, 982, 1014
Ama87I CYCGRG 1 cut(s) 824
AoxI GGCC 6 cut(s) 312, 450, 495, 520, 580, 887
ApeKI GCWGC 7 cut(s) 50, 104, 281, 509, 512, 665, 736
AspLEI GCGC 1 cut(s) 942
AspS9I GGNCC 6 cut(s) 39, 301, 313, 450, 495, 600
AsuC2I CCSGG 2 cut(s) 308, 322
AsuHPI GGTGA 1 cut(s) 563
AsuNHI GCTAGC 1 cut(s) 437
AvaI CYCGRG 1 cut(s) 824
AvaII GGWCC 3 cut(s) 39, 301, 600
BalI TGGCCA 1 cut(s) 582
BanI GGYRCC 1 cut(s) 699
BanII GRGCYC 2 cut(s) 116, 425
BauI CACGAG 1 cut(s) 90
BbrPI CACGTG 1 cut(s) 75
Bbv12I GWGCWC 3 cut(s) 116, 342, 425
BbvCI CCTCAGC 1 cut(s) 855
BbvI GCAGC 7 cut(s) 62, 116, 268, 496, 499, 652, 748
BccI CCATC 3 cut(s) 163, 319, 572
BceAI ACGGC 2 cut(s) 180, 277
BcgI CGANNNNNNTGC 2 cut(s) 96, 130
BciT130I CCWGG 2 cut(s) 672, 758
BcnI CCSGG 2 cut(s) 308, 322
BcoDI GTCTC 1 cut(s) 870
BcuI ACTAGT 1 cut(s) 688
BfaI CTAG 4 cut(s) 438, 524, 560, 689
BglI GCCNNNNNGGC 1 cut(s) 533
BisI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 510, 513, 666, 737
BlpI GCTNAGC 2 cut(s) 17, 904
BlsI GCNGC 9 cut(s) 52, 106, 160, 283, 313, 511, 514, 667, 738
Bme1390I CCNGG 4 cut(s) 308, 322, 672, 758
Bme18I GGWCC 3 cut(s) 39, 301, 600
BmeRI GACNNNNNGTC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 824
BmgBI CACGTC 1 cut(s) 30
BmgT120I GGNCC 6 cut(s) 39, 301, 313, 450, 495, 600
BmiI GGNNCC 2 cut(s) 451, 701
BmrFI CCNGG 4 cut(s) 308, 322, 672, 758
BmsI GCATC 3 cut(s) 14, 544, 545
BmtI GCTAGC 1 cut(s) 441
BplI GAGNNNNNCTC 2 cut(s) 818, 850
BpmI CTGGAG 1 cut(s) 409
Bpu10I CCTNAGC 1 cut(s) 855
Bpu1102I GCTNAGC 2 cut(s) 17, 904
BpuEI CTTGAG 3 cut(s) 501, 800, 824
BpuMI CCSGG 2 cut(s) 308, 322
BsaAI YACGTR 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 870
BsaJI CCNNGG 5 cut(s) 259, 276, 603, 670, 991
BsaWI WCCGGW 1 cut(s) 342
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 93, 123
Bsc4I CCNNNNNNNGG 3 cut(s) 310, 321, 885
Bse118I RCCGGY 1 cut(s) 889
Bse1I ACTGG 1 cut(s) 866
Bse3DI GCAATG 2 cut(s) 778, 981
BseBI CCWGG 2 cut(s) 672, 758
BseDI CCNNGG 5 cut(s) 259, 276, 603, 670, 991
BseGI GGATG 3 cut(s) 330, 403, 536
BseLI CCNNNNNNNGG 3 cut(s) 310, 321, 885
BseMI GCAATG 2 cut(s) 778, 981
BseMII CTCAG 5 cut(s) 8, 351, 464, 846, 918
BseNI ACTGG 1 cut(s) 866
BseRI GAGGAG 2 cut(s) 57, 978
BseXI GCAGC 7 cut(s) 62, 116, 268, 496, 499, 652, 748
BshFI GGCC 6 cut(s) 314, 452, 497, 522, 582, 889
BshNI GGYRCC 1 cut(s) 699
BsiHKAI GWGCWC 3 cut(s) 116, 342, 425
BsiHKCI CYCGRG 1 cut(s) 824
BsiSI CCGG 4 cut(s) 307, 322, 343, 890
BslFI GGGAC 1 cut(s) 462
BslI CCNNNNNNNGG 3 cut(s) 310, 321, 885
BsmAI GTCTC 1 cut(s) 870
BsmFI GGGAC 1 cut(s) 462
BsnI GGCC 6 cut(s) 314, 452, 497, 522, 582, 889
Bso31I GGTCTC 1 cut(s) 870
BsoBI CYCGRG 1 cut(s) 824
Bsp1286I GDGCHC 3 cut(s) 116, 342, 425
Bsp143I GATC 5 cut(s) 235, 589, 709, 987, 1006
Bsp1720I GCTNAGC 2 cut(s) 17, 904
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 3 cut(s) 33, 158, 311
BspANI GGCC 6 cut(s) 314, 452, 497, 522, 582, 889
BspCNI CTCAG 5 cut(s) 9, 352, 465, 847, 917
BspLI GGNNCC 2 cut(s) 451, 701
BspOI GCTAGC 1 cut(s) 441
BspPI GGATC 4 cut(s) 243, 717, 982, 1014
BspT107I GGYRCC 1 cut(s) 699
BspTNI GGTCTC 1 cut(s) 870
BsrDI GCAATG 2 cut(s) 778, 981
BsrFI RCCGGY 1 cut(s) 889
BsrI ACTGG 1 cut(s) 866
BssAI RCCGGY 1 cut(s) 889
BssECI CCNNGG 5 cut(s) 259, 276, 603, 670, 991
BssMI GATC 5 cut(s) 235, 589, 709, 987, 1006
BssSI CACGAG 1 cut(s) 90
BssT1I CCWWGG 3 cut(s) 276, 603, 991
Bst2BI CACGAG 1 cut(s) 90
Bst2UI CCWGG 2 cut(s) 672, 758
Bst6I CTCTTC 1 cut(s) 125
BstBAI YACGTR 1 cut(s) 75
BstC8I GCNNGC 3 cut(s) 48, 439, 891
BstDEI CTNAG 5 cut(s) 17, 360, 473, 855, 904
BstDSI CCRYGG 2 cut(s) 259, 603
BstF5I GGATG 3 cut(s) 330, 403, 536
BstHHI GCGC 1 cut(s) 942
BstKTI GATC 5 cut(s) 238, 592, 712, 990, 1009
BstMAI GTCTC 1 cut(s) 870
BstMBI GATC 5 cut(s) 235, 589, 709, 987, 1006
BstMWI GCNNNNNNNGC 7 cut(s) 158, 164, 173, 494, 509, 533, 674
BstNI CCWGG 2 cut(s) 672, 758
BstNSI RCATGY 3 cut(s) 256, 625, 1035
BstSCI CCNGG 4 cut(s) 306, 320, 670, 756
BstV1I GCAGC 7 cut(s) 62, 116, 268, 496, 499, 652, 748
BstX2I RGATCY 3 cut(s) 235, 709, 1006
BstXI CCANNNNNNTGG 1 cut(s) 433
BstYI RGATCY 3 cut(s) 235, 709, 1006
BsuRI GGCC 6 cut(s) 314, 452, 497, 522, 582, 889
BtgI CCRYGG 2 cut(s) 259, 603
BtgZI GCGATG 1 cut(s) 182
BtrI CACGTC 1 cut(s) 30
BtsCI GGATG 3 cut(s) 330, 403, 536
BtsI GCAGTG 4 cut(s) 114, 159, 183, 900
BtsIMutI CAGTG 4 cut(s) 114, 159, 183, 900
Cac8I GCNNGC 3 cut(s) 48, 439, 891
CfoI GCGC 1 cut(s) 942
Cfr10I RCCGGY 1 cut(s) 889
Cfr13I GGNCC 6 cut(s) 39, 301, 313, 450, 495, 600
Csp6I GTAC 1 cut(s) 482
CviAII CATG 5 cut(s) 253, 604, 622, 696, 1032
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 5 cut(s) 17, 360, 473, 855, 904
DpnI GATC 5 cut(s) 237, 591, 711, 989, 1008
DpnII GATC 5 cut(s) 235, 589, 709, 987, 1006
DriI GACNNNNNGTC 1 cut(s) 217
EaeI YGGCCR 2 cut(s) 580, 887
Eam1104I CTCTTC 1 cut(s) 125
Eam1105I GACNNNNNGTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 125
Ecl136II GAGCTC 2 cut(s) 114, 423
Eco130I CCWWGG 3 cut(s) 276, 603, 991
Eco147I AGGCCT 1 cut(s) 522
Eco24I GRGCYC 2 cut(s) 116, 425
Eco31I GGTCTC 1 cut(s) 870
Eco32I GATATC 2 cut(s) 431, 847
Eco47I GGWCC 3 cut(s) 39, 301, 600
Eco53kI GAGCTC 2 cut(s) 114, 423
Eco57I CTGAAG 1 cut(s) 206
Eco72I CACGTG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 824
EcoICRI GAGCTC 2 cut(s) 114, 423
EcoRII CCWGG 2 cut(s) 670, 756
EcoRV GATATC 2 cut(s) 431, 847
EcoT14I CCWWGG 3 cut(s) 276, 603, 991
EcoT38I GRGCYC 2 cut(s) 116, 425
ErhI CCWWGG 3 cut(s) 276, 603, 991
FaeI CATG 5 cut(s) 256, 607, 625, 699, 1035
FaiI YATR 9 cut(s) 197, 229, 254, 605, 623, 697, 769, 873, 1033
FalI AAGNNNNNCTT 4 cut(s) 524, 556, 911, 943
FaqI GGGAC 1 cut(s) 462
FatI CATG 5 cut(s) 252, 603, 621, 695, 1031
FauI CCCGC 1 cut(s) 26
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 510, 513, 666, 737
FokI GGATG 3 cut(s) 337, 410, 523
FriOI GRGCYC 2 cut(s) 116, 425
Fsp4HI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 510, 513, 666, 737
FspBI CTAG 4 cut(s) 438, 524, 560, 689
FspI TGCGCA 1 cut(s) 941
GlaI GCGC 1 cut(s) 941
GluI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 510, 513, 666, 737
GsuI CTGGAG 1 cut(s) 409
HaeIII GGCC 6 cut(s) 314, 452, 497, 522, 582, 889
HapII CCGG 4 cut(s) 307, 322, 343, 890
HhaI GCGC 1 cut(s) 942
Hin1II CATG 5 cut(s) 256, 607, 625, 699, 1035
Hin6I GCGC 1 cut(s) 940
HinP1I GCGC 1 cut(s) 940
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 5 cut(s) 215, 475, 827, 898, 996
HpaII CCGG 4 cut(s) 307, 322, 343, 890
HphI GGTGA 1 cut(s) 563
Hpy166II GTNNAC 2 cut(s) 97, 331
Hpy188I TCNGA 4 cut(s) 301, 361, 474, 724
Hpy188III TCNNGA 3 cut(s) 426, 575, 1000
Hpy8I GTNNAC 2 cut(s) 97, 331
Hpy99I CGWCG 4 cut(s) 166, 215, 290, 335
HpyAV CCTTC 3 cut(s) 184, 373, 997
HpyCH4IV ACGT 3 cut(s) 29, 74, 288
HpyCH4V TGCA 4 cut(s) 256, 459, 736, 771
HpyF10VI GCNNNNNNNGC 7 cut(s) 158, 164, 173, 494, 509, 533, 674
HpyF3I CTNAG 5 cut(s) 17, 360, 473, 855, 904
HpySE526I ACGT 3 cut(s) 29, 74, 288
Hsp92II CATG 5 cut(s) 256, 607, 625, 699, 1035
HspAI GCGC 1 cut(s) 940
KroI GCCGGC 1 cut(s) 889
KroNI GCCGGC 1 cut(s) 891
Kzo9I GATC 5 cut(s) 235, 589, 709, 987, 1006
LmnI GCTCC 8 cut(s) 101, 181, 345, 420, 428, 674, 759, 965
Lsp1109I GCAGC 7 cut(s) 62, 116, 268, 496, 499, 652, 748
LweI GCATC 3 cut(s) 14, 544, 545
MaeI CTAG 4 cut(s) 438, 524, 560, 689
MaeII ACGT 3 cut(s) 29, 74, 288
MaeIII GTNAC 1 cut(s) 691
MalI GATC 5 cut(s) 237, 591, 711, 989, 1008
MboI GATC 5 cut(s) 235, 589, 709, 987, 1006
MboII GAAGA 8 cut(s) 130, 133, 136, 139, 142, 199, 397, 776
MfeI CAATTG 2 cut(s) 372, 454
MflI RGATCY 3 cut(s) 235, 709, 1006
MhlI GDGCHC 3 cut(s) 116, 342, 425
MlsI TGGCCA 1 cut(s) 582
MluCI AATT 4 cut(s) 372, 454, 584, 1011
MluNI TGGCCA 1 cut(s) 582
MlyI GAGTC 3 cut(s) 224, 484, 836
MmeI TCCRAC 1 cut(s) 279
Mox20I TGGCCA 1 cut(s) 582
MroNI GCCGGC 1 cut(s) 889
MscI TGGCCA 1 cut(s) 582
MseI TTAA 2 cut(s) 918, 1014
Msp20I TGGCCA 1 cut(s) 582
MspI CCGG 4 cut(s) 307, 322, 343, 890
MspR9I CCNGG 4 cut(s) 308, 322, 672, 758
MunI CAATTG 2 cut(s) 372, 454
MvaI CCWGG 2 cut(s) 672, 758
MwoI GCNNNNNNNGC 7 cut(s) 158, 164, 173, 494, 509, 533, 674
NaeI GCCGGC 1 cut(s) 891
NciI CCSGG 2 cut(s) 308, 322
NcoI CCATGG 1 cut(s) 603
NdeII GATC 5 cut(s) 235, 589, 709, 987, 1006
NgoMIV GCCGGC 1 cut(s) 889
NheI GCTAGC 1 cut(s) 437
NlaIII CATG 5 cut(s) 256, 607, 625, 699, 1035
NlaIV GGNNCC 2 cut(s) 451, 701
NmeAIII GCCGAG 1 cut(s) 226
NsbI TGCGCA 1 cut(s) 941
NspI RCATGY 3 cut(s) 256, 625, 1035
PaeR7I CTCGAG 1 cut(s) 824
PceI AGGCCT 1 cut(s) 522
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PdiI GCCGGC 1 cut(s) 891
PfeI GAWTC 2 cut(s) 898, 996
PfoI TCCNGGA 1 cut(s) 756
PkrI GCNGC 9 cut(s) 52, 106, 160, 283, 313, 511, 514, 667, 738
PleI GAGTC 3 cut(s) 223, 483, 835
PmaCI CACGTG 1 cut(s) 75
PmlI CACGTG 1 cut(s) 75
PpsI GAGTC 3 cut(s) 223, 483, 835
Ppu21I YACGTR 1 cut(s) 75
Psp124BI GAGCTC 2 cut(s) 116, 425
Psp6I CCWGG 2 cut(s) 670, 756
PspCI CACGTG 1 cut(s) 75
PspGI CCWGG 2 cut(s) 670, 756
PspN4I GGNNCC 2 cut(s) 451, 701
PspPI GGNCC 6 cut(s) 39, 301, 313, 450, 495, 600
PspXI VCTCGAGB 1 cut(s) 824
PsuI RGATCY 3 cut(s) 235, 709, 1006
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
SacI GAGCTC 2 cut(s) 116, 425
SalI GTCGAC 1 cut(s) 329
SaqAI TTAA 2 cut(s) 918, 1014
SatI GCNGC 9 cut(s) 51, 105, 159, 282, 312, 510, 513, 666, 737
Sau3AI GATC 5 cut(s) 235, 589, 709, 987, 1006
Sau96I GGNCC 6 cut(s) 39, 301, 313, 450, 495, 600
SchI GAGTC 3 cut(s) 224, 484, 836
ScrFI CCNGG 4 cut(s) 308, 322, 672, 758
SduI GDGCHC 3 cut(s) 116, 342, 425
SfaNI GCATC 3 cut(s) 14, 544, 545
Sfr274I CTCGAG 1 cut(s) 824
SinI GGWCC 3 cut(s) 39, 301, 600
SlaI CTCGAG 1 cut(s) 824
SmlI CTYRAG 4 cut(s) 516, 815, 824, 839
SmoI CTYRAG 4 cut(s) 516, 815, 824, 839
SpeI ACTAGT 1 cut(s) 688
Sse9I AATT 4 cut(s) 372, 454, 584, 1011
SseBI AGGCCT 1 cut(s) 522
SsiI CCGC 3 cut(s) 33, 158, 311
SspMI CTAG 4 cut(s) 438, 524, 560, 689
SstI GAGCTC 2 cut(s) 116, 425
StuI AGGCCT 1 cut(s) 522
StyD4I CCNGG 4 cut(s) 306, 320, 670, 756
StyI CCWWGG 3 cut(s) 276, 603, 991
TaiI ACGT 3 cut(s) 32, 77, 291
TaqI TCGA 4 cut(s) 116, 330, 658, 825
TasI AATT 4 cut(s) 372, 454, 584, 1011
TauI GCSGC 2 cut(s) 161, 314
TfiI GAWTC 2 cut(s) 898, 996
Tru1I TTAA 2 cut(s) 918, 1014
Tru9I TTAA 2 cut(s) 918, 1014
TscAI CASTG 4 cut(s) 114, 159, 183, 900
TseI GCWGC 7 cut(s) 50, 104, 281, 509, 512, 665, 736
TspGWI ACGGA 3 cut(s) 114, 858, 1012
TspRI CASTG 4 cut(s) 114, 159, 183, 900
VpaK11BI GGWCC 3 cut(s) 39, 301, 600
XceI RCATGY 3 cut(s) 256, 625, 1035
XcmI CCANNNNNNNNNTGG 1 cut(s) 710
XhoI CTCGAG 1 cut(s) 824
XmiI GTMKAC 1 cut(s) 330
XspI CTAG 4 cut(s) 438, 524, 560, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.