Rh3BG199100
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. gTMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0011326
Physical Location & Seq
Reverse (-)
17670922 .. 17674987
4066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG199100.1

Sequence Viewer

Length: 1017 bp
ATGGCATCTACGAGGCTGAACTACCCGACGTGCCGGTGGTCCTCGCCAGCAGCAACTACGCTTCCTCCTCGCACGTGTCTGGGTGTGGCTGGTGTGAACGGAGCAGCGGTGAGCTCGAAGAAGAAGAAGAAGAGCGGGTTTGAGTTGAGAGCAGTGGCGGCGACGGCGATGGAGGCAGTGGAGCTGAAGAAGGACATAGCCGAGTTCTACGACGAGTCGTCTGGGTTATGGGAGGACCTCTGGGGCGACCACATGCACCACGGCTTCTACGACCCCAAGGCCGCCGACGTTTCGGTGTCGGACCACCGGGCGGCCCAGACCCGGATGATCGACGAGGTGCTACGGTTCGCAGGGATTTCTGACAAGTGTCCAATTGGGAATGTAGTGGATGTTGGGTGTGGGATTGGAGGGAGCTCCAGATATCTGGCTAGCAAATACGGGGCCAATTGCAAAGGGATTACTCTGAGCCCCGTACAGGCTCTGAGGGCCAATGCTCTTGCTGCTGCTCAAGGCCTAGCCAATAAGGCATCCTTTCAAGTTGCTGATGCTCTAGCACAACCATTTCCTGATGGCCAATTTGATCTGGTTTGGTCCATGGAGAGTGGGGAACACATGCCTGATAAAGCAAAGTTTGTTGATGAGTTGGTTCGAGTTGCGGCACCAGGAGCAACGATAATACTAGTTACATGGTGCCACAGGGATCTTGGCGCTTCTGAACAATCATTGCAGCCAGATGAGAAAAAGCTCCTGGACAAGATATGCAATGCTTTCTATCTTCCTGCTTGGTGTTCTACTGCTGACTACCTCAAGCTACTCGAGTCCTACTCACTCAAGGTAATCACTTGGCAGCACTTAAATACCATTAAAGCAAGTCACTTGGGGATGAACCCCATATTTTTGAGGGGTGAACAACTTCGTGTTTCTACTTCGACCTGCATTAAATTGAACCTTCTTTATTGCAGGATATCAAGGCTGAGGACTGGTCTCCGTATGTTGCCCCATTTTGGCCGGCAGTGA

Protein Analysis

338

Amino Acids

36.98

Weight (kDa)

8.73

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 64 - 227 4.7e-11 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 104 - 251 6.8e-13 Methyltransferase domain
Methyltransf_31 PF13847 127 - 245 2e-12 Methyltransferase domain
Methyltransf_25 PF13649 128 - 222 1.5e-17 Methyltransferase domain
Methyltransf_11 PF08241 129 - 226 1.8e-19 Methyltransferase domain
Methyltransf_12 PF08242 129 - 224 2.4e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 941
AccB1I GGYRCC 2 cut(s) 658, 690
AccBSI CCGCTC 1 cut(s) 135
AciI CCGC 6 cut(s) 107, 135, 158, 282, 311, 656
AclWI GGATC 1 cut(s) 708
AcoI YGGCCR 2 cut(s) 571, 1006
AcuI CTGAAG 1 cut(s) 206
AcvI CACGTG 1 cut(s) 75
AfaI GTAC 1 cut(s) 474
AfiI CCNNNNNNNGG 4 cut(s) 310, 321, 475, 1004
AflIII ACRYGT 1 cut(s) 74
AgsI TTSAA 2 cut(s) 536, 946
AhdI GACNNNNNGTC 1 cut(s) 217
AhlI ACTAGT 1 cut(s) 679
AjiI CACGTC 1 cut(s) 30
AjnI CCWGG 2 cut(s) 661, 747
AluBI AGCT 5 cut(s) 114, 184, 414, 745, 811
AluI AGCT 5 cut(s) 114, 184, 414, 745, 811
Alw21I GWGCWC 2 cut(s) 116, 416
Alw26I GTCTC 1 cut(s) 989
AlwI GGATC 1 cut(s) 708
AlwNI CAGNNNCTG 1 cut(s) 481
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 7 cut(s) 279, 312, 441, 486, 511, 571, 1006
ApeKI GCWGC 6 cut(s) 50, 104, 500, 503, 727, 847
Asp700I GAANNNNTTC 1 cut(s) 912
AspLEI GCGC 1 cut(s) 710
AspS9I GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
AsuC2I CCSGG 2 cut(s) 308, 322
AsuHPI GGTGA 2 cut(s) 121, 917
AsuNHI GCTAGC 1 cut(s) 428
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 4 cut(s) 39, 235, 301, 591
BalI TGGCCA 1 cut(s) 573
BanI GGYRCC 2 cut(s) 658, 690
BanII GRGCYC 3 cut(s) 116, 416, 470
BbrPI CACGTG 1 cut(s) 75
Bbv12I GWGCWC 2 cut(s) 116, 416
BbvCI CCTCAGC 1 cut(s) 974
BbvI GCAGC 6 cut(s) 62, 116, 487, 490, 739, 859
BccI CCATC 2 cut(s) 163, 563
BceAI ACGGC 2 cut(s) 180, 277
BciT130I CCWGG 2 cut(s) 663, 749
BcnI CCSGG 2 cut(s) 308, 322
BcoDI GTCTC 1 cut(s) 989
BcuI ACTAGT 1 cut(s) 679
BfaI CTAG 4 cut(s) 429, 515, 551, 680
BfoI RGCGCY 1 cut(s) 711
BfuAI ACCTGC 1 cut(s) 941
BglI GCCNNNNNGGC 1 cut(s) 524
Bme1390I CCNGG 4 cut(s) 308, 322, 663, 749
Bme18I GGWCC 4 cut(s) 39, 235, 301, 591
BmeRI GACNNNNNGTC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 815
BmgBI CACGTC 1 cut(s) 30
BmgT120I GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
BmiI GGNNCC 3 cut(s) 442, 660, 692
BmrFI CCNGG 4 cut(s) 308, 322, 663, 749
BmsI GCATC 3 cut(s) 14, 535, 536
BmtI GCTAGC 1 cut(s) 432
BoxI GACNNNNGTC 1 cut(s) 366
BplI GAGNNNNNCTC 2 cut(s) 809, 841
BpmI CTGGAG 1 cut(s) 400
Bpu10I CCTNAGC 1 cut(s) 974
BpuEI CTTGAG 3 cut(s) 492, 791, 815
BpuMI CCSGG 2 cut(s) 308, 322
BsaAI YACGTR 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 989
BsaJI CCNNGG 3 cut(s) 259, 276, 594
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 93, 123
Bsc4I CCNNNNNNNGG 4 cut(s) 310, 321, 475, 1004
Bse118I RCCGGY 2 cut(s) 33, 1008
Bse1I ACTGG 1 cut(s) 985
Bse3DI GCAATG 2 cut(s) 722, 769
BseBI CCWGG 2 cut(s) 663, 749
BseDI CCNNGG 3 cut(s) 259, 276, 594
BseGI GGATG 4 cut(s) 330, 394, 527, 888
BseLI CCNNNNNNNGG 4 cut(s) 310, 321, 475, 1004
BseMI GCAATG 2 cut(s) 722, 769
BseMII CTCAG 3 cut(s) 455, 473, 965
BseNI ACTGG 1 cut(s) 985
BseRI GAGGAG 1 cut(s) 57
BseXI GCAGC 6 cut(s) 62, 116, 487, 490, 739, 859
BshFI GGCC 7 cut(s) 281, 314, 443, 488, 513, 573, 1008
BshNI GGYRCC 2 cut(s) 658, 690
BsiHKAI GWGCWC 2 cut(s) 116, 416
BsiHKCI CYCGRG 1 cut(s) 815
BsiSI CCGG 4 cut(s) 34, 307, 322, 1009
BslI CCNNNNNNNGG 4 cut(s) 310, 321, 475, 1004
BsmAI GTCTC 1 cut(s) 989
BsnI GGCC 7 cut(s) 281, 314, 443, 488, 513, 573, 1008
Bso31I GGTCTC 1 cut(s) 989
BsoBI CYCGRG 1 cut(s) 815
Bsp1286I GDGCHC 3 cut(s) 116, 416, 470
Bsp143I GATC 3 cut(s) 327, 580, 700
Bsp19I CCATGG 1 cut(s) 594
BspACI CCGC 6 cut(s) 107, 135, 158, 282, 311, 656
BspANI GGCC 7 cut(s) 281, 314, 443, 488, 513, 573, 1008
BspCNI CTCAG 3 cut(s) 456, 474, 966
BspLI GGNNCC 3 cut(s) 442, 660, 692
BspMI ACCTGC 1 cut(s) 941
BspOI GCTAGC 1 cut(s) 432
BspPI GGATC 1 cut(s) 708
BspQI GCTCTTC 1 cut(s) 125
BspT107I GGYRCC 2 cut(s) 658, 690
BspTNI GGTCTC 1 cut(s) 989
BsrBI CCGCTC 1 cut(s) 135
BsrDI GCAATG 2 cut(s) 722, 769
BsrFI RCCGGY 2 cut(s) 33, 1008
BsrI ACTGG 1 cut(s) 985
BssAI RCCGGY 2 cut(s) 33, 1008
BssECI CCNNGG 3 cut(s) 259, 276, 594
BssMI GATC 3 cut(s) 327, 580, 700
BssT1I CCWWGG 2 cut(s) 276, 594
Bst2UI CCWGG 2 cut(s) 663, 749
Bst4CI ACNGT 1 cut(s) 345
Bst6I CTCTTC 1 cut(s) 125
BstBAI YACGTR 1 cut(s) 75
BstC8I GCNNGC 3 cut(s) 48, 430, 1010
BstDEI CTNAG 3 cut(s) 464, 482, 974
BstDSI CCRYGG 2 cut(s) 259, 594
BstF5I GGATG 4 cut(s) 330, 394, 527, 888
BstH2I RGCGCY 1 cut(s) 711
BstHHI GCGC 1 cut(s) 710
BstKTI GATC 3 cut(s) 330, 583, 703
BstMAI GTCTC 1 cut(s) 989
BstMBI GATC 3 cut(s) 327, 580, 700
BstMWI GCNNNNNNNGC 7 cut(s) 158, 164, 173, 485, 500, 524, 665
BstNI CCWGG 2 cut(s) 663, 749
BstNSI RCATGY 2 cut(s) 256, 616
BstPAI GACNNNNGTC 1 cut(s) 366
BstSCI CCNGG 4 cut(s) 306, 320, 661, 747
BstV1I GCAGC 6 cut(s) 62, 116, 487, 490, 739, 859
BstX2I RGATCY 1 cut(s) 700
BstXI CCANNNNNNTGG 1 cut(s) 424
BstYI RGATCY 1 cut(s) 700
BsuRI GGCC 7 cut(s) 281, 314, 443, 488, 513, 573, 1008
BtgI CCRYGG 2 cut(s) 259, 594
BtgZI GCGATG 1 cut(s) 182
BtrI CACGTC 1 cut(s) 30
BtsCI GGATG 4 cut(s) 330, 394, 527, 888
BtsI GCAGTG 2 cut(s) 159, 183
BtsIMutI CAGTG 2 cut(s) 159, 183
BveI ACCTGC 1 cut(s) 941
Cac8I GCNNGC 3 cut(s) 48, 430, 1010
CaiI CAGNNNCTG 1 cut(s) 481
CfoI GCGC 1 cut(s) 710
Cfr10I RCCGGY 2 cut(s) 33, 1008
Cfr13I GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
Csp6I GTAC 1 cut(s) 473
CviAII CATG 4 cut(s) 253, 595, 613, 687
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 3 cut(s) 464, 482, 974
DpnI GATC 3 cut(s) 329, 582, 702
DpnII GATC 3 cut(s) 327, 580, 700
DriI GACNNNNNGTC 1 cut(s) 217
EaeI YGGCCR 2 cut(s) 571, 1006
Eam1104I CTCTTC 1 cut(s) 125
Eam1105I GACNNNNNGTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 125
Ecl136II GAGCTC 2 cut(s) 114, 414
Eco130I CCWWGG 2 cut(s) 276, 594
Eco147I AGGCCT 1 cut(s) 513
Eco24I GRGCYC 3 cut(s) 116, 416, 470
Eco31I GGTCTC 1 cut(s) 989
Eco32I GATATC 2 cut(s) 422, 966
Eco47I GGWCC 4 cut(s) 39, 235, 301, 591
Eco53kI GAGCTC 2 cut(s) 114, 414
Eco57I CTGAAG 1 cut(s) 206
Eco72I CACGTG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 815
EcoICRI GAGCTC 2 cut(s) 114, 414
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 2 cut(s) 661, 747
EcoRV GATATC 2 cut(s) 422, 966
EcoT14I CCWWGG 2 cut(s) 276, 594
EcoT38I GRGCYC 3 cut(s) 116, 416, 470
ErhI CCWWGG 2 cut(s) 276, 594
FaeI CATG 4 cut(s) 256, 598, 616, 690
FaiI YATR 9 cut(s) 197, 229, 254, 596, 614, 688, 760, 893, 992
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FatI CATG 4 cut(s) 252, 594, 612, 686
FauI CCCGC 1 cut(s) 128
FokI GGATG 4 cut(s) 337, 401, 514, 895
FriOI GRGCYC 3 cut(s) 116, 416, 470
FspBI CTAG 4 cut(s) 429, 515, 551, 680
GlaI GCGC 1 cut(s) 709
GsuI CTGGAG 1 cut(s) 400
HaeII RGCGCY 1 cut(s) 711
HaeIII GGCC 7 cut(s) 281, 314, 443, 488, 513, 573, 1008
HapII CCGG 4 cut(s) 34, 307, 322, 1009
HhaI GCGC 1 cut(s) 710
Hin1II CATG 4 cut(s) 256, 598, 616, 690
Hin6I GCGC 1 cut(s) 708
HinP1I GCGC 1 cut(s) 708
HinfI GANTC 2 cut(s) 215, 818
HpaII CCGG 4 cut(s) 34, 307, 322, 1009
HphI GGTGA 2 cut(s) 121, 917
Hpy166II GTNNAC 2 cut(s) 97, 908
Hpy188I TCNGA 5 cut(s) 301, 361, 465, 483, 715
Hpy188III TCNNGA 2 cut(s) 417, 566
Hpy8I GTNNAC 2 cut(s) 97, 908
Hpy99I CGWCG 5 cut(s) 31, 166, 215, 290, 335
HpyAV CCTTC 2 cut(s) 184, 959
HpyCH4III ACNGT 1 cut(s) 345
HpyCH4IV ACGT 3 cut(s) 29, 74, 288
HpyCH4V TGCA 6 cut(s) 256, 450, 727, 762, 936, 960
HpyF10VI GCNNNNNNNGC 7 cut(s) 158, 164, 173, 485, 500, 524, 665
HpyF3I CTNAG 3 cut(s) 464, 482, 974
HpySE526I ACGT 3 cut(s) 29, 74, 288
Hsp92II CATG 4 cut(s) 256, 598, 616, 690
HspAI GCGC 1 cut(s) 708
KroI GCCGGC 1 cut(s) 1008
KroNI GCCGGC 1 cut(s) 1010
Kzo9I GATC 3 cut(s) 327, 580, 700
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 6 cut(s) 101, 181, 411, 419, 665, 750
Lsp1109I GCAGC 6 cut(s) 62, 116, 487, 490, 739, 859
LweI GCATC 3 cut(s) 14, 535, 536
MaeI CTAG 4 cut(s) 429, 515, 551, 680
MaeII ACGT 3 cut(s) 29, 74, 288
MaeIII GTNAC 2 cut(s) 682, 872
MalI GATC 3 cut(s) 329, 582, 702
MbiI CCGCTC 1 cut(s) 135
MboI GATC 3 cut(s) 327, 580, 700
MboII GAAGA 7 cut(s) 130, 133, 136, 139, 142, 199, 767
MfeI CAATTG 2 cut(s) 372, 445
MflI RGATCY 1 cut(s) 700
MhlI GDGCHC 3 cut(s) 116, 416, 470
MlsI TGGCCA 1 cut(s) 573
MluCI AATT 4 cut(s) 372, 445, 575, 941
MluNI TGGCCA 1 cut(s) 573
MlyI GAGTC 2 cut(s) 224, 827
MmeI TCCRAC 1 cut(s) 279
Mox20I TGGCCA 1 cut(s) 573
MroNI GCCGGC 1 cut(s) 1008
MroXI GAANNNNTTC 1 cut(s) 912
MscI TGGCCA 1 cut(s) 573
MseI TTAA 3 cut(s) 854, 864, 939
Msp20I TGGCCA 1 cut(s) 573
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 4 cut(s) 34, 307, 322, 1009
MspR9I CCNGG 4 cut(s) 308, 322, 663, 749
MunI CAATTG 2 cut(s) 372, 445
MvaI CCWGG 2 cut(s) 663, 749
MwoI GCNNNNNNNGC 7 cut(s) 158, 164, 173, 485, 500, 524, 665
NaeI GCCGGC 1 cut(s) 1010
NciI CCSGG 2 cut(s) 308, 322
NcoI CCATGG 1 cut(s) 594
NdeII GATC 3 cut(s) 327, 580, 700
NgoMIV GCCGGC 1 cut(s) 1008
NheI GCTAGC 1 cut(s) 428
NlaIII CATG 4 cut(s) 256, 598, 616, 690
NlaIV GGNNCC 3 cut(s) 442, 660, 692
NmeAIII GCCGAG 1 cut(s) 226
NmuCI GTSAC 1 cut(s) 872
NspI RCATGY 2 cut(s) 256, 616
PaeR7I CTCGAG 1 cut(s) 815
PceI AGGCCT 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 125
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PdiI GCCGGC 1 cut(s) 1010
PdmI GAANNNNTTC 1 cut(s) 912
PfoI TCCNGGA 1 cut(s) 747
PleI GAGTC 2 cut(s) 223, 826
PmaCI CACGTG 1 cut(s) 75
PmlI CACGTG 1 cut(s) 75
PpsI GAGTC 2 cut(s) 223, 826
Ppu21I YACGTR 1 cut(s) 75
PpuMI RGGWCCY 1 cut(s) 235
PshAI GACNNNNGTC 1 cut(s) 366
Psp124BI GAGCTC 2 cut(s) 116, 416
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 2 cut(s) 661, 747
PspCI CACGTG 1 cut(s) 75
PspGI CCWGG 2 cut(s) 661, 747
PspN4I GGNNCC 3 cut(s) 442, 660, 692
PspPI GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
PspPPI RGGWCCY 1 cut(s) 235
PspXI VCTCGAGB 1 cut(s) 815
PstNI CAGNNNCTG 1 cut(s) 481
PsuI RGATCY 1 cut(s) 700
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
SacI GAGCTC 2 cut(s) 116, 416
SapI GCTCTTC 1 cut(s) 125
SaqAI TTAA 3 cut(s) 854, 864, 939
Sau3AI GATC 3 cut(s) 327, 580, 700
Sau96I GGNCC 7 cut(s) 39, 235, 301, 313, 441, 486, 591
SchI GAGTC 2 cut(s) 224, 827
ScrFI CCNGG 4 cut(s) 308, 322, 663, 749
SduI GDGCHC 3 cut(s) 116, 416, 470
SfaNI GCATC 3 cut(s) 14, 535, 536
Sfr274I CTCGAG 1 cut(s) 815
SinI GGWCC 4 cut(s) 39, 235, 301, 591
SlaI CTCGAG 1 cut(s) 815
SmlI CTYRAG 4 cut(s) 507, 806, 815, 830
SmoI CTYRAG 4 cut(s) 507, 806, 815, 830
SpeI ACTAGT 1 cut(s) 679
Sse9I AATT 4 cut(s) 372, 445, 575, 941
SseBI AGGCCT 1 cut(s) 513
SsiI CCGC 6 cut(s) 107, 135, 158, 282, 311, 656
SspMI CTAG 4 cut(s) 429, 515, 551, 680
SstI GAGCTC 2 cut(s) 116, 416
StuI AGGCCT 1 cut(s) 513
StyD4I CCNGG 4 cut(s) 306, 320, 661, 747
StyI CCWWGG 2 cut(s) 276, 594
TaaI ACNGT 1 cut(s) 345
TaiI ACGT 3 cut(s) 32, 77, 291
TaqI TCGA 5 cut(s) 116, 330, 649, 816, 929
TasI AATT 4 cut(s) 372, 445, 575, 941
TauI GCSGC 4 cut(s) 161, 284, 314, 659
Tru1I TTAA 3 cut(s) 854, 864, 939
Tru9I TTAA 3 cut(s) 854, 864, 939
TscAI CASTG 2 cut(s) 159, 183
TseFI GTSAC 1 cut(s) 872
TseI GCWGC 6 cut(s) 50, 104, 500, 503, 727, 847
Tsp45I GTSAC 1 cut(s) 872
TspDTI ATGAA 1 cut(s) 899
TspGWI ACGGA 2 cut(s) 114, 977
TspRI CASTG 2 cut(s) 159, 183
VpaK11BI GGWCC 4 cut(s) 39, 235, 301, 591
XceI RCATGY 2 cut(s) 256, 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 701
XhoI CTCGAG 1 cut(s) 815
XmnI GAANNNNTTC 1 cut(s) 912
XspI CTAG 4 cut(s) 429, 515, 551, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.