AT1G65320

CBS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010953
Physical Location & Seq
Reverse (-)
24259880 .. 24262998
3119 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65320.1

Sequence Viewer

Length: 1278 bp
ATGGCGTCAGTGTTCTTGTATCATGTGGTGGGAGATTTAACTGTAGGAAAGCCAGAGATGGTTGAGTTCTACGAAACAGAGACGGTGGAATCTGCGATTAGAGCTATCGGAGAATCGACGGAGTGTGGGATTCCGGTTTGGAGGAAGAGAACGACACCTTCGCTTCCTGGGTTTGTTGAGAATAGTGAGATGAGACAACAAAGATTCGTTGGGATTTTGAATTCTTTAGATATCGTTGCGTTTTTGGCTAAGACTGAGTGTTTGCAAGAAGAAAAAGCTATGAAGATTCCTGTCTCTGAGGTTGTTTCTCCTGACAATACTCTATTGAAACAGGTTGATCCTGGGACTAGGTTGATAGATGCTTTGGAGATGATGAAACAAGGAGTGAGACGACTTCTGGTTCCAAAAAGCGTTGTTTGGAGAGGTATGAGCAAGAGGTTCTCCATTCTTTACAATGGAAAATGGCTGAAGAACAGTGAAAACTCTAGTAGTAGCAGTGGTCTCTCTGCTGACTCCACCAACAGGCCTACTACGTCCATGACTTCAAGCCGGGACAAGTTTTGTTGTCTCTCTAGGGAAGACGTGATCCGCTTCCTTATAGGTGTCCTTGGAGCATTAGCTCCTTTACCACTCACATCAATCTCCACTCTGGGAATCATCAATCAAAACTATAACTTCATTGAAGCCTCTCTTCCAGCCATTGAAGCCACTCGTAGGCCACTGTGTGACCCTAGTGCGATCGCCGTGTTGGAACAAACCGAAAACGAACAACAGTTCAAGATAATAGGAGAAATCTCAGCTTCAAAGCTCTGGAAATGTGATTATCTAGCTGCAGCTTGGGCTCTAGCTAATCTCTATGCAGGACAATTTGTGATGGGAGTTGAGGACAACATGTCATCAAGATCATTCAGCGACTTCCTCCAAACTTCATTTCCAGGAGGAGAACAGAACGGAACAGCAACAAATGCCAAGAAGTTCAGCAGCAGGAGCATCGGTTTTAATCCGACAAGCCCGACAAGATTGAGCATCGGTAGGAGCATGTACAGAGGAAGAAGTGCGCCGCTAACTTGCAAAACATCGAGCTCATTAGCTGCTGTTATGGCTCAGATGCTGTCTCACAGGGCTACTCATGTTTGGGTTACAGAGGCTGATTCAGATGATGTTCTTGTTGGAGTTGTGGGCTATGGAGAGATCTTGACTGCTGTAACTAAACAACCTTCTGCTTTCGTTCCTTCGAATCGGTCTTATGAAGGTTTTGGCAATGAAAATCAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS

Protein Analysis

425

Amino Acids

46.55

Weight (kDa)

6.85

Isoelectric Point (pI)

52.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010953)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65320
fragaria_vesca FvH4_6g00690 FvH4_6g00690 FvH4_6g00690
malus_domestica MD04G1243500.v1.1 MD12G1259100.v1.1
prunus_persica Prupe.6G359700_v2.0.a1
pyrus_communis pycom12g23760
rosa_chinensis RchiOBHm_Chr3g0448131
rosa_laevigata RLG00000025944 RLG00000026020
rosa_multiflora Rmu_sc0005969.1_g000029
rosa_roxburghii Rroxscaffold_6G00426260
rosa_rugosa Rorug02G0608300 Rorug02G0608400
rosa_samantha Rh3AG008200 Rh3BG007700 Rh3CG007700 Rh3DG008300
rosa_wichuraiana Rw3G000640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 589, 1061
AclWI GGATC 2 cut(s) 332, 580
AcsI RAATTY 1 cut(s) 220
AcuI CTGAAG 1 cut(s) 488
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 725
AfaI GTAC 1 cut(s) 1043
AfiI CCNNNNNNNGG 2 cut(s) 522, 714
AflIII ACRYGT 1 cut(s) 891
AgsI TTSAA 7 cut(s) 220, 328, 546, 683, 704, 778, 804
AhdI GACNNNNNGTC 1 cut(s) 892
AjiI CACGTC 1 cut(s) 583
AjnI CCWGG 3 cut(s) 166, 340, 934
AjuI GAANNNNNNNTTGG 2 cut(s) 915, 947
Alw21I GWGCWC 1 cut(s) 1085
Alw26I GTCTC 7 cut(s) 74, 187, 298, 382, 506, 572, 1119
AlwI GGATC 2 cut(s) 332, 580
AlwNI CAGNNNCTG 2 cut(s) 1111, 1148
AoxI GGCC 2 cut(s) 524, 716
ApeKI GCWGC 4 cut(s) 830, 833, 981, 1091
ApoI RAATTY 1 cut(s) 220
ArsI GACNNNNNNTTYG 2 cut(s) 244, 276
AsiSI GCGATCGC 1 cut(s) 741
AspLEI GCGC 1 cut(s) 1060
AsuC2I CCSGG 1 cut(s) 551
AsuII TTCGAA 1 cut(s) 1235
BanII GRGCYC 2 cut(s) 844, 1085
BbsI GAAGAC 1 cut(s) 585
Bbv12I GWGCWC 1 cut(s) 1085
BbvI GCAGC 4 cut(s) 817, 845, 993, 1078
BccI CCATC 2 cut(s) 52, 868
BceAI ACGGC 1 cut(s) 728
BcgI CGANNNNNNTGC 2 cut(s) 973, 1007
BciT130I CCWGG 3 cut(s) 168, 342, 936
BcnI CCSGG 1 cut(s) 551
BcoDI GTCTC 7 cut(s) 74, 187, 298, 382, 506, 572, 1119
BfaI CTAG 6 cut(s) 348, 486, 573, 732, 827, 845
BfmI CTRYAG 2 cut(s) 42, 831
BglII AGATCT 1 cut(s) 1191
BisI GCNGC 5 cut(s) 831, 834, 982, 1061, 1092
BlsI GCNGC 5 cut(s) 832, 835, 983, 1062, 1093
Bme1390I CCNGG 4 cut(s) 168, 342, 551, 936
BmeRI GACNNNNNGTC 1 cut(s) 892
BmgBI CACGTC 1 cut(s) 583
BmiI GGNNCC 1 cut(s) 402
BmrFI CCNGG 4 cut(s) 168, 342, 551, 936
BmsI GCATC 4 cut(s) 349, 999, 1035, 1098
BpiI GAAGAC 1 cut(s) 585
Bpu14I TTCGAA 1 cut(s) 1235
BpuMI CCSGG 1 cut(s) 551
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 506
BsaJI CCNNGG 3 cut(s) 167, 341, 607
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 2 cut(s) 979, 1009
Bsc4I CCNNNNNNNGG 2 cut(s) 522, 714
Bse3DI GCAATG 1 cut(s) 1267
BseBI CCWGG 3 cut(s) 168, 342, 936
BseDI CCNNGG 3 cut(s) 167, 341, 607
BseLI CCNNNNNNNGG 2 cut(s) 522, 714
BseMI GCAATG 1 cut(s) 1267
BseMII CTCAG 4 cut(s) 246, 288, 810, 1118
BseRI GAGGAG 1 cut(s) 954
BseXI GCAGC 4 cut(s) 817, 845, 993, 1078
Bsh1285I CGRYCG 1 cut(s) 741
BshFI GGCC 2 cut(s) 526, 718
BsiEI CGRYCG 1 cut(s) 741
BsiHKAI GWGCWC 1 cut(s) 1085
BsiSI CCGG 2 cut(s) 134, 550
BslFI GGGAC 2 cut(s) 358, 566
BslI CCNNNNNNNGG 2 cut(s) 522, 714
BsmAI GTCTC 7 cut(s) 74, 187, 298, 382, 506, 572, 1119
BsmBI CGTCTC 2 cut(s) 74, 382
BsmFI GGGAC 2 cut(s) 358, 566
BsnI GGCC 2 cut(s) 526, 718
Bso31I GGTCTC 1 cut(s) 506
Bsp119I TTCGAA 1 cut(s) 1235
Bsp1286I GDGCHC 2 cut(s) 844, 1085
Bsp1407I TGTACA 1 cut(s) 1041
Bsp143I GATC 5 cut(s) 337, 585, 738, 902, 1191
BspACI CCGC 2 cut(s) 589, 1061
BspANI GGCC 2 cut(s) 526, 718
BspCNI CTCAG 4 cut(s) 247, 289, 809, 1117
BspLI GGNNCC 1 cut(s) 402
BspMAI CTGCAG 1 cut(s) 835
BspPI GGATC 2 cut(s) 332, 580
BspT104I TTCGAA 1 cut(s) 1235
BspTNI GGTCTC 1 cut(s) 506
BsrDI GCAATG 1 cut(s) 1267
BsrGI TGTACA 1 cut(s) 1041
BssECI CCNNGG 3 cut(s) 167, 341, 607
BssMI GATC 5 cut(s) 337, 585, 738, 902, 1191
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 607
Bst2UI CCWGG 3 cut(s) 168, 342, 936
Bst4CI ACNGT 5 cut(s) 43, 85, 476, 723, 774
Bst6I CTCTTC 2 cut(s) 140, 696
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 965
BstAUI TGTACA 1 cut(s) 1041
BstBI TTCGAA 1 cut(s) 1235
BstDEI CTNAG 5 cut(s) 249, 255, 297, 796, 1104
BstHHI GCGC 1 cut(s) 1060
BstKTI GATC 5 cut(s) 340, 588, 741, 905, 1194
BstMAI GTCTC 7 cut(s) 74, 187, 298, 382, 506, 572, 1119
BstMBI GATC 5 cut(s) 337, 585, 738, 902, 1191
BstMCI CGRYCG 1 cut(s) 741
BstMWI GCNNNNNNNGC 7 cut(s) 101, 245, 704, 839, 965, 987, 1100
BstNI CCWGG 3 cut(s) 168, 342, 936
BstNSI RCATGY 2 cut(s) 895, 1042
BstSCI CCNGG 4 cut(s) 166, 340, 549, 934
BstSFI CTRYAG 2 cut(s) 42, 831
BstV1I GCAGC 4 cut(s) 817, 845, 993, 1078
BstV2I GAAGAC 1 cut(s) 585
BstX2I RGATCY 1 cut(s) 1191
BstYI RGATCY 1 cut(s) 1191
BsuRI GGCC 2 cut(s) 526, 718
BtrI CACGTC 1 cut(s) 583
BtsI GCAGTG 1 cut(s) 502
BtsIMutI CAGTG 4 cut(s) 15, 481, 502, 719
CaiI CAGNNNCTG 2 cut(s) 1111, 1148
CfoI GCGC 1 cut(s) 1060
Csp6I GTAC 1 cut(s) 1042
CviAII CATG 5 cut(s) 23, 538, 892, 1039, 1130
CviQI GTAC 1 cut(s) 1042
DdeI CTNAG 5 cut(s) 249, 255, 297, 796, 1104
DpnI GATC 5 cut(s) 339, 587, 740, 904, 1193
DpnII GATC 5 cut(s) 337, 585, 738, 902, 1191
DraIII CACNNNGTG 1 cut(s) 725
DriI GACNNNNNGTC 1 cut(s) 892
Eam1104I CTCTTC 2 cut(s) 140, 696
Eam1105I GACNNNNNGTC 1 cut(s) 892
EarI CTCTTC 2 cut(s) 140, 696
Ecl136II GAGCTC 1 cut(s) 1083
Eco130I CCWWGG 1 cut(s) 607
Eco147I AGGCCT 1 cut(s) 526
Eco24I GRGCYC 2 cut(s) 844, 1085
Eco31I GGTCTC 1 cut(s) 506
Eco32I GATATC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 1083
Eco57I CTGAAG 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 1083
EcoRI GAATTC 1 cut(s) 220
EcoRII CCWGG 3 cut(s) 166, 340, 934
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 607
EcoT38I GRGCYC 2 cut(s) 844, 1085
ErhI CCWWGG 1 cut(s) 607
Esp3I CGTCTC 2 cut(s) 74, 382
FaeI CATG 5 cut(s) 26, 541, 895, 1042, 1133
FalI AAGNNNNNCTT 2 cut(s) 675, 707
FaqI GGGAC 2 cut(s) 358, 566
FatI CATG 5 cut(s) 22, 537, 891, 1038, 1129
Fnu4HI GCNGC 5 cut(s) 831, 834, 982, 1061, 1092
FriOI GRGCYC 2 cut(s) 844, 1085
Fsp4HI GCNGC 5 cut(s) 831, 834, 982, 1061, 1092
FspBI CTAG 6 cut(s) 348, 486, 573, 732, 827, 845
GlaI GCGC 1 cut(s) 1059
GluI GCNGC 5 cut(s) 831, 834, 982, 1061, 1092
HaeIII GGCC 2 cut(s) 526, 718
HapII CCGG 2 cut(s) 134, 550
HhaI GCGC 1 cut(s) 1060
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 26, 541, 895, 1042, 1133
Hin6I GCGC 1 cut(s) 1058
HinP1I GCGC 1 cut(s) 1058
HinfI GANTC 9 cut(s) 89, 113, 130, 204, 286, 512, 654, 1151, 1237
HpaII CCGG 2 cut(s) 134, 550
Hpy188I TCNGA 5 cut(s) 110, 298, 1005, 1107, 1156
Hpy188III TCNNGA 5 cut(s) 311, 778, 811, 900, 1195
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 4 cut(s) 168, 1227, 1242, 1244
HpyCH4III ACNGT 5 cut(s) 43, 85, 476, 723, 774
HpyCH4IV ACGT 2 cut(s) 533, 582
HpyCH4V TGCA 4 cut(s) 265, 833, 860, 1071
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 245, 704, 839, 965, 987, 1100
HpyF3I CTNAG 5 cut(s) 249, 255, 297, 796, 1104
HpySE526I ACGT 2 cut(s) 533, 582
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 26, 541, 895, 1042, 1133
HspAI GCGC 1 cut(s) 1058
Kzo9I GATC 5 cut(s) 337, 585, 738, 902, 1191
LmnI GCTCC 4 cut(s) 611, 625, 987, 1035
Lsp1109I GCAGC 4 cut(s) 817, 845, 993, 1078
LweI GCATC 4 cut(s) 349, 999, 1035, 1098
MaeI CTAG 6 cut(s) 348, 486, 573, 732, 827, 845
MaeII ACGT 2 cut(s) 533, 582
MaeIII GTNAC 3 cut(s) 725, 1138, 1204
MalI GATC 5 cut(s) 339, 587, 740, 904, 1193
MboI GATC 5 cut(s) 337, 585, 738, 902, 1191
MboII GAAGA 7 cut(s) 157, 281, 295, 481, 590, 683, 1062
MflI RGATCY 1 cut(s) 1191
MhlI GDGCHC 2 cut(s) 844, 1085
MluCI AATT 2 cut(s) 220, 866
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 3 cut(s) 729, 1028, 1150
MseI TTAA 2 cut(s) 38, 999
MspI CCGG 2 cut(s) 134, 550
MspR9I CCNGG 4 cut(s) 168, 342, 551, 936
MvaI CCWGG 3 cut(s) 168, 342, 936
MwoI GCNNNNNNNGC 7 cut(s) 101, 245, 704, 839, 965, 987, 1100
NciI CCSGG 1 cut(s) 551
NdeII GATC 5 cut(s) 337, 585, 738, 902, 1191
NlaIII CATG 5 cut(s) 26, 541, 895, 1042, 1133
NlaIV GGNNCC 1 cut(s) 402
NmuCI GTSAC 1 cut(s) 725
NspI RCATGY 2 cut(s) 895, 1042
NspV TTCGAA 1 cut(s) 1235
PceI AGGCCT 1 cut(s) 526
PciI ACATGT 1 cut(s) 891
PfeI GAWTC 8 cut(s) 89, 113, 130, 204, 286, 654, 1151, 1237
PfoI TCCNGGA 1 cut(s) 934
PkrI GCNGC 5 cut(s) 832, 835, 983, 1062, 1093
Ple19I CGATCG 1 cut(s) 741
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
PscI ACATGT 1 cut(s) 891
Psp124BI GAGCTC 1 cut(s) 1085
Psp6I CCWGG 3 cut(s) 166, 340, 934
PspGI CCWGG 3 cut(s) 166, 340, 934
PspN4I GGNNCC 1 cut(s) 402
PstI CTGCAG 1 cut(s) 835
PstNI CAGNNNCTG 2 cut(s) 1111, 1148
PsuI RGATCY 1 cut(s) 1191
PvuI CGATCG 1 cut(s) 741
RgaI GCGATCGC 1 cut(s) 741
RsaI GTAC 1 cut(s) 1043
RsaNI GTAC 1 cut(s) 1042
SacI GAGCTC 1 cut(s) 1085
SaqAI TTAA 2 cut(s) 38, 999
SatI GCNGC 5 cut(s) 831, 834, 982, 1061, 1092
Sau3AI GATC 5 cut(s) 337, 585, 738, 902, 1191
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 4 cut(s) 168, 342, 551, 936
SduI GDGCHC 2 cut(s) 844, 1085
SfaAI GCGATCGC 1 cut(s) 741
SfaNI GCATC 4 cut(s) 349, 999, 1035, 1098
SfcI CTRYAG 2 cut(s) 42, 831
SfuI TTCGAA 1 cut(s) 1235
SgfI GCGATCGC 1 cut(s) 741
Sse9I AATT 2 cut(s) 220, 866
SseBI AGGCCT 1 cut(s) 526
SsiI CCGC 2 cut(s) 589, 1061
SspMI CTAG 6 cut(s) 348, 486, 573, 732, 827, 845
SstI GAGCTC 1 cut(s) 1085
StuI AGGCCT 1 cut(s) 526
StyD4I CCNGG 4 cut(s) 166, 340, 549, 934
StyI CCWWGG 1 cut(s) 607
TaaI ACNGT 5 cut(s) 43, 85, 476, 723, 774
TaiI ACGT 2 cut(s) 536, 585
TaqI TCGA 3 cut(s) 116, 1079, 1235
TaqII GACCGA 1 cut(s) 1230
TasI AATT 2 cut(s) 220, 866
TatI WGTACW 1 cut(s) 1041
TauI GCSGC 1 cut(s) 1063
TfiI GAWTC 8 cut(s) 89, 113, 130, 204, 286, 654, 1151, 1237
Tru1I TTAA 2 cut(s) 38, 999
Tru9I TTAA 2 cut(s) 38, 999
TscAI CASTG 4 cut(s) 15, 481, 502, 726
TseFI GTSAC 1 cut(s) 725
TseI GCWGC 4 cut(s) 830, 833, 981, 1091
Tsp45I GTSAC 1 cut(s) 725
TspDTI ATGAA 6 cut(s) 296, 389, 667, 918, 1263, 1278
TspGWI ACGGA 2 cut(s) 134, 966
TspRI CASTG 4 cut(s) 15, 481, 502, 726
XapI RAATTY 1 cut(s) 220
XceI RCATGY 2 cut(s) 895, 1042
XspI CTAG 6 cut(s) 348, 486, 573, 732, 827, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.