Rh3AG008200

CBS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0010953
Physical Location & Seq
Forward (+)
530407 .. 533169
2763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG008200.1

Sequence Viewer

Length: 1281 bp
ATGGCATCGGTGTTTCTGTACCATGTGGTGGCTGATCTAACGGTCGGGAAGCCGGAGCTGGTGGAGTTCTGCGAGACGGAGACGGTGGAGGCGGCGATAAAGGCGATCGGAGACTCGACTGAGTGTGGCATACCGGTGTGGAAGAGGAAAACGCATGCGGGTATTGAGAACACTGAAATGAGGCAGCAGAGGTTTGTGGGGATTCTCAACTCGCTTGACATTGTTGCTTTCTTTGCTAAAAGTGAGTGCTTGGAGGATCATGACAAGGCTCTTAAGACTCCGGTTTCTGAAGTTGTTGCTCATAATAATTCCTTGCTGCGCCAGGTTGATCCTGGGACAAGGTTGATAGATGCTCTGGAGATGATGAAGCATGGTGTAAAGCGTCTTCTTGTTCGAAAGAGTGTCGTATGGCAAGGCATGAGCAAGCGCTTCTCAATTCTCTATAATGGTAAGTGGCTCAAGAATATGGATACTAGCAGCAGCAGCAATAACCTTGGTCTCAGTTCCAGTCGGCCTTCCTCATCAACCACTACCAGCGCCCGCGACAAATTTTGCTGTCTCTCAAGAGAAGATGTTATACGTTTCCTAATTGGTTGCCTGGGTGCCCTAGCACCTCTTCCGCTCTCCTCTATCTCCTCGGTTGGAGCTATCAACCCAAACTACAACTCGATTGAAGCCACATCTGCAGCCATTGAAGCCACTCAGAAACTTCCTGAAGACCCCTGTGCAATTGCTGTTCTTGAACAGTCGCAGGATGGTCAGTATAAGATTATTGGAGAGATCTCTGCCTCCAAACTATGGAAATGTGATCACTTAGCAGCAGCATGGGCTTTAGCTAATCTCTCCGGTGGACAATTTGTAATGGGAGTTGAGGATAATGTGTCCTCAAGGTCACTGAAAGATTTTTCTGTAAATCAAGCTGCTGGGAATCACAATCTAGCTAATGGTGGTGCGTCAACAAAACCAAAGAAATTCAGTAGCAGGAGTATAGGTTTCAACGCTGCAAACCCGAGCTTGGCAGTGAGTAGAAGCATGTACAGGGGTAGAAGTGCACCGTTGACATGTAAGGTTACAAGTTCATTGGCTGCTGTTATGGCTCAGATGTTGTCTCACAGAGCAACCCATGTCTGGGTGACTGAGGATGAAAGTGATGATGTTTTAGTTGGAGTCGTTGGTTATGCGGATATTATGGTTGCTGTCACCAAACAACCATCCTCCGTTGTCCCTCCACCAGGTCGGTCTACCGAGAATTCTGGTATTGTTAATGACGTCCACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS

Protein Analysis

426

Amino Acids

45.84

Weight (kDa)

7.6

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010953)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65320
fragaria_vesca FvH4_6g00690 FvH4_6g00690 FvH4_6g00690
malus_domestica MD04G1243500.v1.1 MD12G1259100.v1.1
prunus_persica Prupe.6G359700_v2.0.a1
pyrus_communis pycom12g23760
rosa_chinensis RchiOBHm_Chr3g0448131
rosa_laevigata RLG00000025944 RLG00000026020
rosa_multiflora Rmu_sc0005969.1_g000029
rosa_roxburghii Rroxscaffold_6G00426260
rosa_rugosa Rorug02G0608300 Rorug02G0608400
rosa_samantha Rh3AG008200 Rh3BG007700 Rh3CG007700 Rh3DG008300
rosa_wichuraiana Rw3G000640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1272
AccB1I GGYRCC 1 cut(s) 602
AccB7I CCANNNNNTGG 2 cut(s) 28, 798
AccBSI CCGCTC 1 cut(s) 622
AccI GTMKAC 1 cut(s) 1241
AccII CGCG 1 cut(s) 543
AciI CCGC 5 cut(s) 92, 158, 541, 620, 1181
AclWI GGATC 2 cut(s) 264, 323
AcsI RAATTY 3 cut(s) 548, 971, 1249
AcuI CTGAAG 2 cut(s) 309, 735
AcyI GRCGYC 1 cut(s) 1269
AfaI GTAC 2 cut(s) 20, 1037
AfeI AGCGCT 1 cut(s) 428
AfiI CCNNNNNNNGG 6 cut(s) 28, 798, 1015, 1128, 1129, 1232
AflII CTTAAG 1 cut(s) 272
AflIII ACRYGT 1 cut(s) 1061
AgeI ACCGGT 1 cut(s) 133
AgsI TTSAA 4 cut(s) 674, 695, 743, 997
AjnI CCWGG 4 cut(s) 321, 331, 597, 1231
AluBI AGCT 6 cut(s) 58, 647, 836, 920, 941, 1014
AluI AGCT 6 cut(s) 58, 647, 836, 920, 941, 1014
Alw21I GWGCWC 1 cut(s) 1054
Alw26I GTCTC 6 cut(s) 68, 74, 105, 503, 563, 1113
Alw44I GTGCAC 1 cut(s) 1050
AlwI GGATC 2 cut(s) 264, 323
Ama87I CYCGRG 1 cut(s) 1009
Aor51HI AGCGCT 1 cut(s) 428
AoxI GGCC 1 cut(s) 512
ApaLI GTGCAC 1 cut(s) 1050
ApoI RAATTY 3 cut(s) 548, 971, 1249
AsiGI ACCGGT 1 cut(s) 133
AspLEI GCGC 3 cut(s) 321, 429, 539
AsuHPI GGTGA 2 cut(s) 1144, 1192
AsuII TTCGAA 1 cut(s) 394
AvaI CYCGRG 1 cut(s) 1009
BaeGI GKGCMC 2 cut(s) 607, 1054
BanI GGYRCC 1 cut(s) 602
BarI GAAGNNNNNNTAC 4 cut(s) 369, 401, 561, 593
BbsI GAAGAC 2 cut(s) 377, 723
Bbv12I GWGCWC 1 cut(s) 1054
BccI CCATC 2 cut(s) 749, 1219
BciT130I CCWGG 4 cut(s) 323, 333, 599, 1233
BciVI GTATCC 1 cut(s) 463
BclI TGATCA 1 cut(s) 808
BcoDI GTCTC 6 cut(s) 68, 74, 105, 503, 563, 1113
BfaI CTAG 3 cut(s) 474, 608, 938
BfmI CTRYAG 1 cut(s) 684
BfoI RGCGCY 2 cut(s) 430, 540
BfrI CTTAAG 1 cut(s) 272
BfuI GTATCC 1 cut(s) 463
BglII AGATCT 1 cut(s) 780
Bme1390I CCNGG 4 cut(s) 323, 333, 599, 1233
BmeT110I CYCGRG 1 cut(s) 1009
BmiI GGNNCC 1 cut(s) 604
BmrFI CCNGG 4 cut(s) 323, 333, 599, 1233
BmsI GCATC 2 cut(s) 14, 340
BpiI GAAGAC 2 cut(s) 377, 723
BpmI CTGGAG 1 cut(s) 377
Bpu14I TTCGAA 1 cut(s) 394
BpuEI CTTGAG 3 cut(s) 443, 547, 871
BsaHI GRCGYC 1 cut(s) 1269
BsaI GGTCTC 1 cut(s) 503
BsaJI CCNNGG 4 cut(s) 332, 493, 598, 636
BsaWI WCCGGW 3 cut(s) 133, 280, 845
BsaXI ACNNNNNCTCC 2 cut(s) 976, 1006
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 798, 1015, 1128, 1129, 1232
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 1 cut(s) 507
BseBI CCWGG 4 cut(s) 323, 333, 599, 1233
BseDI CCNNGG 4 cut(s) 332, 493, 598, 636
BseGI GGATG 3 cut(s) 760, 1147, 1211
BseLI CCNNNNNNNGG 6 cut(s) 28, 798, 1015, 1128, 1129, 1232
BseMII CTCAG 5 cut(s) 111, 514, 716, 1112, 1128
BseNI ACTGG 1 cut(s) 507
BseRI GAGGAG 2 cut(s) 616, 625
BseSI GKGCMC 2 cut(s) 607, 1054
BseYI CCCAGC 1 cut(s) 923
Bsh1236I CGCG 1 cut(s) 543
Bsh1285I CGRYCG 2 cut(s) 45, 108
BshFI GGCC 1 cut(s) 514
BshNI GGYRCC 1 cut(s) 602
BshTI ACCGGT 1 cut(s) 133
BsiEI CGRYCG 2 cut(s) 45, 108
BsiHKAI GWGCWC 1 cut(s) 1054
BsiHKCI CYCGRG 1 cut(s) 1009
BsiSI CCGG 4 cut(s) 53, 134, 281, 846
BslFI GGGAC 2 cut(s) 349, 1208
BslI CCNNNNNNNGG 6 cut(s) 28, 798, 1015, 1128, 1129, 1232
BsmAI GTCTC 6 cut(s) 68, 74, 105, 503, 563, 1113
BsmBI CGTCTC 2 cut(s) 68, 74
BsmFI GGGAC 2 cut(s) 349, 1208
BsnI GGCC 1 cut(s) 514
Bso31I GGTCTC 1 cut(s) 503
BsoBI CYCGRG 1 cut(s) 1009
Bsp119I TTCGAA 1 cut(s) 394
Bsp1286I GDGCHC 2 cut(s) 607, 1054
Bsp1407I TGTACA 1 cut(s) 1035
Bsp143I GATC 6 cut(s) 34, 105, 256, 328, 780, 808
BspACI CCGC 5 cut(s) 92, 158, 541, 620, 1181
BspANI GGCC 1 cut(s) 514
BspCNI CTCAG 5 cut(s) 112, 513, 715, 1111, 1129
BspFNI CGCG 1 cut(s) 543
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 1 cut(s) 604
BspMAI CTGCAG 1 cut(s) 688
BspPI GGATC 2 cut(s) 264, 323
BspT104I TTCGAA 1 cut(s) 394
BspT107I GGYRCC 1 cut(s) 602
BspTI CTTAAG 1 cut(s) 272
BspTNI GGTCTC 1 cut(s) 503
BsrBI CCGCTC 1 cut(s) 622
BsrFI RCCGGY 1 cut(s) 133
BsrGI TGTACA 1 cut(s) 1035
BsrI ACTGG 1 cut(s) 507
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 4 cut(s) 332, 493, 598, 636
BssMI GATC 6 cut(s) 34, 105, 256, 328, 780, 808
BssNI GRCGYC 1 cut(s) 1269
BssT1I CCWWGG 1 cut(s) 493
Bst2UI CCWGG 4 cut(s) 323, 333, 599, 1233
Bst4CI ACNGT 4 cut(s) 43, 85, 747, 1056
Bst6I CTCTTC 2 cut(s) 137, 621
BstACI GRCGYC 1 cut(s) 1269
BstAFI CTTAAG 1 cut(s) 272
BstAUI TGTACA 1 cut(s) 1035
BstBI TTCGAA 1 cut(s) 394
BstC8I GCNNGC 3 cut(s) 156, 425, 541
BstDEI CTNAG 6 cut(s) 120, 500, 702, 814, 1098, 1137
BstENI CCTNNNNNAGG 1 cut(s) 1230
BstF5I GGATG 3 cut(s) 760, 1147, 1211
BstFNI CGCG 1 cut(s) 543
BstH2I RGCGCY 2 cut(s) 430, 540
BstHHI GCGC 3 cut(s) 321, 429, 539
BstKTI GATC 6 cut(s) 37, 108, 259, 331, 783, 811
BstMAI GTCTC 6 cut(s) 68, 74, 105, 503, 563, 1113
BstMBI GATC 6 cut(s) 34, 105, 256, 328, 780, 808
BstMCI CGRYCG 2 cut(s) 45, 108
BstMWI GCNNNNNNNGC 7 cut(s) 101, 233, 483, 683, 695, 827, 1094
BstNI CCWGG 4 cut(s) 323, 333, 599, 1233
BstNSI RCATGY 3 cut(s) 158, 1036, 1065
BstSCI CCNGG 4 cut(s) 321, 331, 597, 1231
BstSFI CTRYAG 1 cut(s) 684
BstSLI GKGCMC 2 cut(s) 607, 1054
BstUI CGCG 1 cut(s) 543
BstV2I GAAGAC 2 cut(s) 377, 723
BstX2I RGATCY 1 cut(s) 780
BstYI RGATCY 1 cut(s) 780
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 1 cut(s) 514
BtsCI GGATG 3 cut(s) 760, 1147, 1211
BtsI GCAGTG 1 cut(s) 1026
BtsIMutI CAGTG 3 cut(s) 171, 893, 1026
Cac8I GCNNGC 3 cut(s) 156, 425, 541
CciI TCATGA 1 cut(s) 259
CfoI GCGC 3 cut(s) 321, 429, 539
Cfr10I RCCGGY 1 cut(s) 133
CseI GACGC 2 cut(s) 371, 942
CsiI ACCWGGT 1 cut(s) 1231
Csp6I GTAC 2 cut(s) 19, 1036
CspAI ACCGGT 1 cut(s) 133
CviAII CATG 9 cut(s) 23, 155, 260, 371, 418, 825, 1033, 1062, 1124
CviQI GTAC 2 cut(s) 19, 1036
DdeI CTNAG 6 cut(s) 120, 500, 702, 814, 1098, 1137
DpnI GATC 6 cut(s) 36, 107, 258, 330, 782, 810
DpnII GATC 6 cut(s) 34, 105, 256, 328, 780, 808
Eam1104I CTCTTC 2 cut(s) 137, 621
EarI CTCTTC 2 cut(s) 137, 621
Eco130I CCWWGG 1 cut(s) 493
Eco31I GGTCTC 1 cut(s) 503
Eco47III AGCGCT 1 cut(s) 428
Eco57I CTGAAG 2 cut(s) 309, 735
Eco88I CYCGRG 1 cut(s) 1009
EcoNI CCTNNNNNAGG 1 cut(s) 1230
EcoRI GAATTC 1 cut(s) 1249
EcoRII CCWGG 4 cut(s) 321, 331, 597, 1231
EcoT14I CCWWGG 1 cut(s) 493
ErhI CCWWGG 1 cut(s) 493
Esp3I CGTCTC 2 cut(s) 68, 74
FaeI CATG 9 cut(s) 26, 158, 263, 374, 421, 828, 1036, 1065, 1127
FaqI GGGAC 2 cut(s) 349, 1208
FatI CATG 9 cut(s) 22, 154, 259, 370, 417, 824, 1032, 1061, 1123
FauI CCCGC 2 cut(s) 151, 548
FbaI TGATCA 1 cut(s) 808
FblI GTMKAC 1 cut(s) 1241
FokI GGATG 3 cut(s) 767, 1154, 1198
FspBI CTAG 3 cut(s) 474, 608, 938
GlaI GCGC 3 cut(s) 320, 428, 538
GsaI CCCAGC 1 cut(s) 927
GsuI CTGGAG 1 cut(s) 377
HaeII RGCGCY 2 cut(s) 430, 540
HaeIII GGCC 1 cut(s) 514
HapII CCGG 4 cut(s) 53, 134, 281, 846
HgaI GACGC 2 cut(s) 371, 942
HhaI GCGC 3 cut(s) 321, 429, 539
Hin1I GRCGYC 1 cut(s) 1269
Hin1II CATG 9 cut(s) 26, 158, 263, 374, 421, 828, 1036, 1065, 1127
Hin6I GCGC 3 cut(s) 319, 427, 537
HinP1I GCGC 3 cut(s) 319, 427, 537
HincII GTYRAC 2 cut(s) 957, 1059
HindII GTYRAC 2 cut(s) 957, 1059
HinfI GANTC 5 cut(s) 113, 202, 277, 928, 1167
HpaII CCGG 4 cut(s) 53, 134, 281, 846
HphI GGTGA 2 cut(s) 1144, 1192
Hpy166II GTNNAC 6 cut(s) 851, 957, 1052, 1059, 1242, 1273
Hpy188I TCNGA 4 cut(s) 110, 289, 705, 1101
Hpy188III TCNNGA 7 cut(s) 46, 260, 356, 460, 564, 713, 740
Hpy8I GTNNAC 6 cut(s) 851, 957, 1052, 1059, 1242, 1273
HpyAV CCTTC 1 cut(s) 525
HpyCH4III ACNGT 4 cut(s) 43, 85, 747, 1056
HpyCH4IV ACGT 2 cut(s) 580, 1269
HpyCH4V TGCA 4 cut(s) 686, 728, 1004, 1052
HpyF10VI GCNNNNNNNGC 7 cut(s) 101, 233, 483, 683, 695, 827, 1094
HpyF3I CTNAG 6 cut(s) 120, 500, 702, 814, 1098, 1137
HpySE526I ACGT 2 cut(s) 580, 1269
Hsp92I GRCGYC 1 cut(s) 1269
Hsp92II CATG 9 cut(s) 26, 158, 263, 374, 421, 828, 1036, 1065, 1127
HspAI GCGC 3 cut(s) 319, 427, 537
Ksp22I TGATCA 1 cut(s) 808
Kzo9I GATC 6 cut(s) 34, 105, 256, 328, 780, 808
LmnI GCTCC 2 cut(s) 55, 644
LweI GCATC 2 cut(s) 14, 340
MabI ACCWGGT 1 cut(s) 1231
MaeI CTAG 3 cut(s) 474, 608, 938
MaeII ACGT 2 cut(s) 580, 1269
MaeIII GTNAC 4 cut(s) 891, 1069, 1132, 1198
MalI GATC 6 cut(s) 36, 107, 258, 330, 782, 810
MbiI CCGCTC 1 cut(s) 622
MboI GATC 6 cut(s) 34, 105, 256, 328, 780, 808
MboII GAAGA 5 cut(s) 154, 377, 581, 608, 728
MfeI CAATTG 2 cut(s) 729, 1276
MflI RGATCY 1 cut(s) 780
MhlI GDGCHC 2 cut(s) 607, 1054
MluCI AATT 9 cut(s) 307, 435, 548, 588, 729, 854, 971, 1249, 1276
MlyI GAGTC 3 cut(s) 107, 271, 1176
MmeI TCCRAC 2 cut(s) 622, 1144
MseI TTAA 2 cut(s) 273, 1263
MslI CAYNNNNRTG 2 cut(s) 134, 176
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 4 cut(s) 53, 134, 281, 846
MspR9I CCNGG 4 cut(s) 323, 333, 599, 1233
MunI CAATTG 2 cut(s) 729, 1276
MvaI CCWGG 4 cut(s) 323, 333, 599, 1233
MvnI CGCG 1 cut(s) 543
MwoI GCNNNNNNNGC 7 cut(s) 101, 233, 483, 683, 695, 827, 1094
NdeII GATC 6 cut(s) 34, 105, 256, 328, 780, 808
NlaIII CATG 9 cut(s) 26, 158, 263, 374, 421, 828, 1036, 1065, 1127
NlaIV GGNNCC 1 cut(s) 604
NmuCI GTSAC 3 cut(s) 891, 1132, 1198
NspI RCATGY 3 cut(s) 158, 1036, 1065
NspV TTCGAA 1 cut(s) 394
PaeI GCATGC 1 cut(s) 158
PagI TCATGA 1 cut(s) 259
PciI ACATGT 1 cut(s) 1061
PfeI GAWTC 2 cut(s) 202, 928
PflMI CCANNNNNTGG 2 cut(s) 28, 798
PinAI ACCGGT 1 cut(s) 133
Ple19I CGATCG 1 cut(s) 108
PleI GAGTC 3 cut(s) 107, 271, 1175
PpsI GAGTC 3 cut(s) 107, 271, 1175
PscI ACATGT 1 cut(s) 1061
Psp6I CCWGG 4 cut(s) 321, 331, 597, 1231
PspFI CCCAGC 1 cut(s) 923
PspGI CCWGG 4 cut(s) 321, 331, 597, 1231
PspN4I GGNNCC 1 cut(s) 604
PstI CTGCAG 1 cut(s) 688
PsuI RGATCY 1 cut(s) 780
PvuI CGATCG 1 cut(s) 108
RsaI GTAC 2 cut(s) 20, 1037
RsaNI GTAC 2 cut(s) 19, 1036
RseI CAYNNNNRTG 2 cut(s) 134, 176
SaqAI TTAA 2 cut(s) 273, 1263
Sau3AI GATC 6 cut(s) 34, 105, 256, 328, 780, 808
SchI GAGTC 3 cut(s) 107, 271, 1176
ScrFI CCNGG 4 cut(s) 323, 333, 599, 1233
SduI GDGCHC 2 cut(s) 607, 1054
SexAI ACCWGGT 1 cut(s) 1231
SfaNI GCATC 2 cut(s) 14, 340
SfcI CTRYAG 1 cut(s) 684
SfuI TTCGAA 1 cut(s) 394
SmiMI CAYNNNNRTG 2 cut(s) 134, 176
SmlI CTYRAG 4 cut(s) 272, 458, 562, 886
SmoI CTYRAG 4 cut(s) 272, 458, 562, 886
SphI GCATGC 1 cut(s) 158
Sse9I AATT 9 cut(s) 307, 435, 548, 588, 729, 854, 971, 1249, 1276
SsiI CCGC 5 cut(s) 92, 158, 541, 620, 1181
SspMI CTAG 3 cut(s) 474, 608, 938
StyD4I CCNGG 4 cut(s) 321, 331, 597, 1231
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 4 cut(s) 43, 85, 747, 1056
TaiI ACGT 2 cut(s) 583, 1272
TaqI TCGA 3 cut(s) 116, 394, 668
TaqII GACCGA 1 cut(s) 1227
TasI AATT 9 cut(s) 307, 435, 548, 588, 729, 854, 971, 1249, 1276
TatI WGTACW 1 cut(s) 1035
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 2 cut(s) 202, 928
Tru1I TTAA 2 cut(s) 273, 1263
Tru9I TTAA 2 cut(s) 273, 1263
TscAI CASTG 3 cut(s) 178, 900, 1026
TseFI GTSAC 3 cut(s) 891, 1132, 1198
Tsp45I GTSAC 3 cut(s) 891, 1132, 1198
TspDTI ATGAA 3 cut(s) 380, 1068, 1158
TspGWI ACGGA 2 cut(s) 92, 1207
TspRI CASTG 3 cut(s) 178, 900, 1026
Van91I CCANNNNNTGG 2 cut(s) 28, 798
Vha464I CTTAAG 1 cut(s) 272
VneI GTGCAC 1 cut(s) 1050
XagI CCTNNNNNAGG 1 cut(s) 1230
XapI RAATTY 3 cut(s) 548, 971, 1249
XceI RCATGY 3 cut(s) 158, 1036, 1065
XcmI CCANNNNNNNNNTGG 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 1241
XspI CTAG 3 cut(s) 474, 608, 938
ZraI GACGTC 1 cut(s) 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.