MD04G1243500.v1.1

CBS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010953
Physical Location & Seq
Reverse (-)
31983086 .. 31986013
2928 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1243500.v1.1.491

Sequence Viewer

Length: 1230 bp
ATGGTGGAGCTCTGCGAGACGGAGACGATGGAGGCGGCCATAAGGGCGATCGGAGAGTCGACGGAATGCGGCATACCGGTGTGGAAGAGGAAATCGAATGTGGGAATGGTCGAAAACAATGAAATGTTGCAGCAGAGGTTTGTGGGAATCCTTAATTCCCTCGACATTGTTGCTTTCTTTGCTAAAAGTGAGTGCCTTGAGGATCATGACAAGGCTTTGAAGACTCCGGTGTCTGATGTTGTTGTTCCGAATAACTCTTTGCTCAAGCAGGTTGATCCTGCCACAAGCGTGCTGCTGTTGATAGATGCACTGGAGATGATGAAGCATGGTGTAAAGCGTCTTATTGTTCGAAAGAGTGTGGTATGGAAAGGCATGAGCAAACGTTTCTCTATGATCTATAGTGGTAAGTGGCTCAAGAACATAGATGCTAGTGGCAGCAGCAATAACCTTGCTGCCAATCCCAATCGGCCTTCCTCATCTTCCACTAGCAGTACCCGTGACAAGTTTTGCTGTCTCTCAAGAGAAGATGTCATCCGTTTCCTGATTGGTTGCCTAGGTGCCCTAGCACCTCTTCCACTCTCCTCTATCTCCTCTATTGGAGCAATTAACCCAAACTACCAATTTGTTGAAGCCTCGGCTGCAGCCATTGAAGCCACTAAAAAGCTTCCAGACGACCCTAGTGCAGTTGCTGTTGTCGAACAGACACAGGATGATGAGTATAAGATCATCGGAGAGATCTCTGCCTCCAAACTATGGAAATGTGATTACTTATCAGCAGCATGGGCCTTAGCTAATCTCTCCGCTGGACAGTTTGTGATGGGAGTTGAGGATAATGTGTCCTCAAGGTCCGAATACAATTTCTCTGTGCATCCCACTGCTGGAAATAATAACCAAGCTAATGGTGGCGGGTCAGCAAGGCCGAAGAAGTTCAGTAGCAGGAGTATAGGGTTCAATCCTGCGAGCCCAAGCTTGGGAGTGAGCAGAAGCATGTATAGGGGAAGAAGTGCACCCTTGACCTGTAGGGTTACGAGTTCATTGGCGGCAGTGATGGCTCAGATGTTGTCTCACAGGGCAACACACGTGTGGGTGACCCAGGATCATAGTGATGATATCGTCGTTGGAGTGGTGGGTTATGCAGATATTATGGCTGCTGTCACCAAACAACCAGCCTCCACAAACCCTGCACCAGGGCGGACAACTGAGGTTGCTGGTAATGACACTCACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS

Protein Analysis

410

Amino Acids

44.01

Weight (kDa)

7.59

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010953)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65320
fragaria_vesca FvH4_6g00690 FvH4_6g00690 FvH4_6g00690
malus_domestica MD04G1243500.v1.1 MD12G1259100.v1.1
prunus_persica Prupe.6G359700_v2.0.a1
pyrus_communis pycom12g23760
rosa_chinensis RchiOBHm_Chr3g0448131
rosa_laevigata RLG00000025944 RLG00000026020
rosa_multiflora Rmu_sc0005969.1_g000029
rosa_roxburghii Rroxscaffold_6G00426260
rosa_rugosa Rorug02G0608300 Rorug02G0608400
rosa_samantha Rh3AG008200 Rh3BG007700 Rh3CG007700 Rh3DG008300
rosa_wichuraiana Rw3G000640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 229
Acc36I ACCTGC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 557
AccB7I CCANNNNNTGG 1 cut(s) 753
AccI GTMKAC 1 cut(s) 59
AciI CCGC 6 cut(s) 35, 69, 801, 906, 1040, 1192
AclI AACGTT 1 cut(s) 382
AclWI GGATC 3 cut(s) 210, 269, 1104
AcoI YGGCCR 1 cut(s) 36
AcvI CACGTG 1 cut(s) 1081
AfaI GTAC 1 cut(s) 493
AfiI CCNNNNNNNGG 5 cut(s) 753, 878, 970, 971, 1187
AflIII ACRYGT 2 cut(s) 1078, 1080
AgeI ACCGGT 1 cut(s) 76
AgsI TTSAA 4 cut(s) 220, 629, 650, 952
AjnI CCWGG 2 cut(s) 1092, 1186
AleI CACNNNNGTG 2 cut(s) 287, 1081
AluBI AGCT 5 cut(s) 10, 664, 791, 896, 969
AluI AGCT 5 cut(s) 10, 664, 791, 896, 969
Alw21I GWGCWC 2 cut(s) 12, 1009
Alw26I GTCTC 4 cut(s) 11, 17, 518, 1068
Alw44I GTGCAC 1 cut(s) 1005
AlwI GGATC 3 cut(s) 210, 269, 1104
AlwNI CAGNNNCTG 1 cut(s) 689
AoxI GGCC 4 cut(s) 36, 467, 783, 917
ApaLI GTGCAC 1 cut(s) 1005
ApeKI GCWGC 9 cut(s) 130, 292, 435, 438, 452, 638, 641, 776, 1148
AsiGI ACCGGT 1 cut(s) 76
Asp700I GAANNNNTTC 1 cut(s) 926
AspA2I CCTAGG 1 cut(s) 553
AspS9I GGNCC 2 cut(s) 783, 846
AsuHPI GGTGA 3 cut(s) 1099, 1147, 1214
AsuII TTCGAA 1 cut(s) 349
AvaII GGWCC 1 cut(s) 846
AvrII CCTAGG 1 cut(s) 553
BaeGI GKGCMC 2 cut(s) 562, 1009
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 2 cut(s) 12, 965
BbrPI CACGTG 1 cut(s) 1081
BbsI GAAGAC 1 cut(s) 227
Bbv12I GWGCWC 2 cut(s) 12, 1009
BbvI GCAGC 9 cut(s) 142, 279, 439, 447, 450, 625, 653, 788, 1135
BccI CCATC 3 cut(s) 22, 811, 1042
BcgI CGANNNNNNTGC 4 cut(s) 152, 186, 662, 696
BciT130I CCWGG 2 cut(s) 1094, 1188
BcoDI GTCTC 4 cut(s) 11, 17, 518, 1068
BfaI CTAG 5 cut(s) 429, 486, 554, 563, 678
BfmI CTRYAG 3 cut(s) 397, 639, 1018
BfuAI ACCTGC 1 cut(s) 259
BglI GCCNNNNNGGC 1 cut(s) 44
BglII AGATCT 1 cut(s) 735
BlnI CCTAGG 1 cut(s) 553
Bme1390I CCNGG 2 cut(s) 1094, 1188
Bme18I GGWCC 1 cut(s) 846
BmgT120I GGNCC 2 cut(s) 783, 846
BmiI GGNNCC 1 cut(s) 559
BmrFI CCNGG 2 cut(s) 1094, 1188
BmsI GCATC 3 cut(s) 295, 415, 877
BpiI GAAGAC 1 cut(s) 227
BpmI CTGGAG 1 cut(s) 332
Bpu10I CCTNAGC 1 cut(s) 787
Bpu14I TTCGAA 1 cut(s) 349
BpuEI CTTGAG 5 cut(s) 218, 248, 398, 502, 826
BsaAI YACGTR 1 cut(s) 1081
BsaJI CCNNGG 4 cut(s) 553, 633, 1092, 1187
BsaWI WCCGGW 2 cut(s) 76, 226
Bsc4I CCNNNNNNNGG 5 cut(s) 753, 878, 970, 971, 1187
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 315
BseBI CCWGG 2 cut(s) 1094, 1188
BseDI CCNNGG 4 cut(s) 553, 633, 1092, 1187
BseGI GGATG 3 cut(s) 531, 715, 868
BseLI CCNNNNNNNGG 5 cut(s) 753, 878, 970, 971, 1187
BseMII CTCAG 2 cut(s) 1067, 1191
BseNI ACTGG 1 cut(s) 315
BseRI GAGGAG 2 cut(s) 571, 580
BseSI GKGCMC 2 cut(s) 562, 1009
BseXI GCAGC 9 cut(s) 142, 279, 439, 447, 450, 625, 653, 788, 1135
BsgI GTGCAG 2 cut(s) 702, 1167
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 4 cut(s) 38, 469, 785, 919
BshNI GGYRCC 1 cut(s) 557
BshTI ACCGGT 1 cut(s) 76
BsiEI CGRYCG 1 cut(s) 51
BsiHKAI GWGCWC 2 cut(s) 12, 1009
BsiSI CCGG 2 cut(s) 77, 227
BslI CCNNNNNNNGG 5 cut(s) 753, 878, 970, 971, 1187
BsmAI GTCTC 4 cut(s) 11, 17, 518, 1068
BsmBI CGTCTC 2 cut(s) 11, 17
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 4 cut(s) 38, 469, 785, 919
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 4 cut(s) 12, 562, 965, 1009
Bsp143I GATC 7 cut(s) 48, 202, 274, 393, 723, 735, 1096
BspACI CCGC 6 cut(s) 35, 69, 801, 906, 1040, 1192
BspANI GGCC 4 cut(s) 38, 469, 785, 919
BspCNI CTCAG 2 cut(s) 1066, 1192
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 559
BspMAI CTGCAG 1 cut(s) 643
BspMI ACCTGC 1 cut(s) 259
BspPI GGATC 3 cut(s) 210, 269, 1104
BspT104I TTCGAA 1 cut(s) 349
BspT107I GGYRCC 1 cut(s) 557
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 315
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 4 cut(s) 553, 633, 1092, 1187
BssMI GATC 7 cut(s) 48, 202, 274, 393, 723, 735, 1096
BssT1I CCWWGG 1 cut(s) 553
Bst2UI CCWGG 2 cut(s) 1094, 1188
Bst4CI ACNGT 1 cut(s) 810
Bst6I CTCTTC 2 cut(s) 80, 576
BstBAI YACGTR 1 cut(s) 1081
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 2 cut(s) 290, 961
BstDEI CTNAG 3 cut(s) 787, 1053, 1200
BstEII GGTNACC 1 cut(s) 1087
BstENI CCTNNNNNAGG 1 cut(s) 1185
BstF5I GGATG 3 cut(s) 531, 715, 868
BstKTI GATC 7 cut(s) 51, 205, 277, 396, 726, 738, 1099
BstMAI GTCTC 4 cut(s) 11, 17, 518, 1068
BstMBI GATC 7 cut(s) 48, 202, 274, 393, 723, 735, 1096
BstMCI CGRYCG 1 cut(s) 51
BstMWI GCNNNNNNNGC 6 cut(s) 44, 179, 638, 650, 782, 1049
BstNI CCWGG 2 cut(s) 1094, 1188
BstNSI RCATGY 1 cut(s) 991
BstPI GGTNACC 1 cut(s) 1087
BstSCI CCNGG 2 cut(s) 1092, 1186
BstSFI CTRYAG 3 cut(s) 397, 639, 1018
BstSLI GKGCMC 2 cut(s) 562, 1009
BstV1I GCAGC 9 cut(s) 142, 279, 439, 447, 450, 625, 653, 788, 1135
BstV2I GAAGAC 1 cut(s) 227
BstX2I RGATCY 1 cut(s) 735
BstXI CCANNNNNNTGG 1 cut(s) 899
BstYI RGATCY 1 cut(s) 735
BsuRI GGCC 4 cut(s) 38, 469, 785, 919
BtsCI GGATG 3 cut(s) 531, 715, 868
BtsI GCAGTG 2 cut(s) 873, 1050
BtsIMutI CAGTG 3 cut(s) 308, 873, 1050
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 2 cut(s) 290, 961
CaiI CAGNNNCTG 1 cut(s) 689
CciI TCATGA 1 cut(s) 205
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 783, 846
CseI GACGC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 492
CspAI ACCGGT 1 cut(s) 76
CviAII CATG 5 cut(s) 206, 326, 373, 780, 988
CviQI GTAC 1 cut(s) 492
DdeI CTNAG 3 cut(s) 787, 1053, 1200
DpnI GATC 7 cut(s) 50, 204, 276, 395, 725, 737, 1098
DpnII GATC 7 cut(s) 48, 202, 274, 393, 723, 735, 1096
DrdI GACNNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 2 cut(s) 80, 576
EarI CTCTTC 2 cut(s) 80, 576
EciI GGCGGA 1 cut(s) 1207
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 1 cut(s) 553
Eco24I GRGCYC 2 cut(s) 12, 965
Eco32I GATATC 1 cut(s) 1111
Eco47I GGWCC 1 cut(s) 846
Eco53kI GAGCTC 1 cut(s) 10
Eco72I CACGTG 1 cut(s) 1081
Eco91I GGTNACC 1 cut(s) 1087
EcoICRI GAGCTC 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 1185
EcoO65I GGTNACC 1 cut(s) 1087
EcoRII CCWGG 2 cut(s) 1092, 1186
EcoRV GATATC 1 cut(s) 1111
EcoT14I CCWWGG 1 cut(s) 553
EcoT38I GRGCYC 2 cut(s) 12, 965
ErhI CCWWGG 1 cut(s) 553
Esp3I CGTCTC 2 cut(s) 11, 17
FaeI CATG 5 cut(s) 209, 329, 376, 783, 991
FatI CATG 5 cut(s) 205, 325, 372, 779, 987
FauI CCCGC 1 cut(s) 899
FblI GTMKAC 1 cut(s) 59
FokI GGATG 3 cut(s) 518, 722, 855
FriOI GRGCYC 2 cut(s) 12, 965
FspBI CTAG 5 cut(s) 429, 486, 554, 563, 678
GsuI CTGGAG 1 cut(s) 332
HaeIII GGCC 4 cut(s) 38, 469, 785, 919
HapII CCGG 2 cut(s) 77, 227
HgaI GACGC 1 cut(s) 326
Hin1II CATG 5 cut(s) 209, 329, 376, 783, 991
HincII GTYRAC 1 cut(s) 60
HindII GTYRAC 1 cut(s) 60
HindIII AAGCTT 2 cut(s) 662, 967
HinfI GANTC 3 cut(s) 56, 147, 223
HpaII CCGG 2 cut(s) 77, 227
HphI GGTGA 3 cut(s) 1099, 1147, 1214
Hpy166II GTNNAC 2 cut(s) 60, 1007
Hpy188I TCNGA 6 cut(s) 53, 235, 249, 731, 850, 1056
Hpy188III TCNNGA 5 cut(s) 206, 415, 519, 541, 668
Hpy8I GTNNAC 2 cut(s) 60, 1007
Hpy99I CGWCG 2 cut(s) 64, 1118
HpyAV CCTTC 1 cut(s) 480
HpyCH4III ACNGT 1 cut(s) 810
HpyCH4IV ACGT 2 cut(s) 382, 1080
HpyCH4V TGCA 8 cut(s) 130, 308, 641, 683, 868, 1007, 1136, 1184
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 179, 638, 650, 782, 1049
HpyF3I CTNAG 3 cut(s) 787, 1053, 1200
HpySE526I ACGT 2 cut(s) 382, 1080
Hsp92II CATG 5 cut(s) 209, 329, 376, 783, 991
Kzo9I GATC 7 cut(s) 48, 202, 274, 393, 723, 735, 1096
LmnI GCTCC 2 cut(s) 7, 599
Lsp1109I GCAGC 9 cut(s) 142, 279, 439, 447, 450, 625, 653, 788, 1135
LweI GCATC 3 cut(s) 295, 415, 877
MaeI CTAG 5 cut(s) 429, 486, 554, 563, 678
MaeII ACGT 2 cut(s) 382, 1080
MaeIII GTNAC 4 cut(s) 497, 1024, 1087, 1153
MalI GATC 7 cut(s) 50, 204, 276, 395, 725, 737, 1098
MboI GATC 7 cut(s) 48, 202, 274, 393, 723, 735, 1096
MboII GAAGA 7 cut(s) 97, 232, 471, 536, 563, 934, 1011
MflI RGATCY 1 cut(s) 735
MhlI GDGCHC 4 cut(s) 12, 562, 965, 1009
MluCI AATT 4 cut(s) 154, 603, 620, 856
MlyI GAGTC 2 cut(s) 65, 217
MmeI TCCRAC 1 cut(s) 1099
MroXI GAANNNNTTC 1 cut(s) 926
MseI TTAA 2 cut(s) 153, 606
MslI CAYNNNNRTG 4 cut(s) 77, 287, 1081, 1104
MspA1I CMGCKG 1 cut(s) 803
MspI CCGG 2 cut(s) 77, 227
MspR9I CCNGG 2 cut(s) 1094, 1188
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 2 cut(s) 1094, 1188
MwoI GCNNNNNNNGC 6 cut(s) 44, 179, 638, 650, 782, 1049
NdeII GATC 7 cut(s) 48, 202, 274, 393, 723, 735, 1096
NlaIII CATG 5 cut(s) 209, 329, 376, 783, 991
NlaIV GGNNCC 1 cut(s) 559
NmeAIII GCCGAG 1 cut(s) 614
NmuCI GTSAC 3 cut(s) 497, 1087, 1153
NspI RCATGY 1 cut(s) 991
NspV TTCGAA 1 cut(s) 349
OliI CACNNNNGTG 2 cut(s) 287, 1081
PagI TCATGA 1 cut(s) 205
PctI GAATGC 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 926
PfeI GAWTC 1 cut(s) 147
PflMI CCANNNNNTGG 1 cut(s) 753
PinAI ACCGGT 1 cut(s) 76
Ple19I CGATCG 1 cut(s) 51
PleI GAGTC 2 cut(s) 64, 217
PmaCI CACGTG 1 cut(s) 1081
PmlI CACGTG 1 cut(s) 1081
PpsI GAGTC 2 cut(s) 64, 217
Ppu21I YACGTR 1 cut(s) 1081
Psp124BI GAGCTC 1 cut(s) 12
Psp1406I AACGTT 1 cut(s) 382
Psp6I CCWGG 2 cut(s) 1092, 1186
PspCI CACGTG 1 cut(s) 1081
PspEI GGTNACC 1 cut(s) 1087
PspGI CCWGG 2 cut(s) 1092, 1186
PspN4I GGNNCC 1 cut(s) 559
PspPI GGNCC 2 cut(s) 783, 846
PstI CTGCAG 1 cut(s) 643
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 1 cut(s) 735
PvuI CGATCG 1 cut(s) 51
RsaI GTAC 1 cut(s) 493
RsaNI GTAC 1 cut(s) 492
RseI CAYNNNNRTG 4 cut(s) 77, 287, 1081, 1104
SacI GAGCTC 1 cut(s) 12
SalI GTCGAC 1 cut(s) 58
SaqAI TTAA 2 cut(s) 153, 606
Sau3AI GATC 7 cut(s) 48, 202, 274, 393, 723, 735, 1096
Sau96I GGNCC 2 cut(s) 783, 846
SchI GAGTC 2 cut(s) 65, 217
ScrFI CCNGG 2 cut(s) 1094, 1188
SduI GDGCHC 4 cut(s) 12, 562, 965, 1009
SfaNI GCATC 3 cut(s) 295, 415, 877
SfcI CTRYAG 3 cut(s) 397, 639, 1018
SfuI TTCGAA 1 cut(s) 349
SinI GGWCC 1 cut(s) 846
SmiMI CAYNNNNRTG 4 cut(s) 77, 287, 1081, 1104
SmlI CTYRAG 5 cut(s) 197, 263, 413, 517, 841
SmoI CTYRAG 5 cut(s) 197, 263, 413, 517, 841
Sse9I AATT 4 cut(s) 154, 603, 620, 856
SsiI CCGC 6 cut(s) 35, 69, 801, 906, 1040, 1192
SspMI CTAG 5 cut(s) 429, 486, 554, 563, 678
SstI GAGCTC 1 cut(s) 12
StyD4I CCNGG 2 cut(s) 1092, 1186
StyI CCWWGG 1 cut(s) 553
TaaI ACNGT 1 cut(s) 810
TaiI ACGT 2 cut(s) 385, 1083
TaqI TCGA 6 cut(s) 59, 95, 111, 162, 349, 696
TasI AATT 4 cut(s) 154, 603, 620, 856
TauI GCSGC 3 cut(s) 38, 72, 1043
TfiI GAWTC 1 cut(s) 147
Tru1I TTAA 2 cut(s) 153, 606
Tru9I TTAA 2 cut(s) 153, 606
TscAI CASTG 3 cut(s) 315, 880, 1050
TseFI GTSAC 3 cut(s) 497, 1087, 1153
TseI GCWGC 9 cut(s) 130, 292, 435, 438, 452, 638, 641, 776, 1148
Tsp45I GTSAC 3 cut(s) 497, 1087, 1153
TspDTI ATGAA 3 cut(s) 135, 335, 1023
TspGWI ACGGA 3 cut(s) 35, 77, 524
TspRI CASTG 3 cut(s) 315, 880, 1050
Van91I CCANNNNNTGG 1 cut(s) 753
VneI GTGCAC 1 cut(s) 1005
VpaK11BI GGWCC 1 cut(s) 846
XagI CCTNNNNNAGG 1 cut(s) 1185
XceI RCATGY 1 cut(s) 991
XcmI CCANNNNNNNNNTGG 1 cut(s) 899
XmaJI CCTAGG 1 cut(s) 553
XmiI GTMKAC 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 926
XspI CTAG 5 cut(s) 429, 486, 554, 563, 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.