AT1G65440

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009494
Physical Location & Seq
Reverse (-)
24306545 .. 24314639
8095 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65440.2

Sequence Viewer

Length: 4944 bp
ATGGCGAGGAACGCAATCTCTGACGATGAAGAGGATCATGAGCTTGAGGATGACGACGGAGAGCCAGTTCATGGAGATCCAGCTGAGCATGATGAAAACGATGATGAAGAAGATGATGATGACGTTGGGAATGAGTATGAGAATGATGGGTTCATAGTGAATGATGAAGACGAAGAGGAAGAAGAGGAAGAAGACGAAGAAAGGAAAGATAGCGATGAGGAGAGGCAGAAAAAGAAGAAGAAACGAAAGAAAAAGGACGAAGGTCTTGATGAAGATGATTATTTGCTTCTACAAGATAACAACGTCAAATTCAAAAAGAGGCAGTATAAGCGGTTGAAGAAAGCTCAAAGGGAACAGGGAAATGGCCAGGGAGAATCATCCGATGATGAGTTTGATAGTAGAGGTGGAACTAGGAGAAGTGCTGAGGATAAGATCAAAGACAGGCTGTTTGATGATGTTGATGTTGATGATCCACCTGATGATGTAGGTGATGAGGAAGACTTAGTTGTGGAAGAAGATGTAGTTGGGAGCGAGGATGAAATGGCAGATTTTATTGTAGATGAAGATGATGAGCATGGTCCTCCTAAGAGGGGCAACTCGAAGAAAAAGAAATATAGGCAGGGATCAGATATTACTGCTATGCGAGATGCTAATGAAATATTTGGTGATGTTGACGAATTATTAACAATTCGCAAAAAGGGTTTGGCATCTAATCAAAGGATGGAAAGAAGGCTCGAAGATGAGTTTGAACCAACTGTTCTTTCTGAGAAATATATGACAGGGAATGATGATGAAATCCGCCAGCTTGATATTCCTGAGAGAATGCAGATATCTGAAGAAAGCACCGGAAGCCCTCCTGTAGATGAAATTAGCATTGAAGAAGAAAGCAATTGGATATATGCTCAACTTGCTTCCCAACTTAGAGAATCTGATGGAACTTTTGATGGGCGGGGTTTCTCGGTTAATAAGGATGACATCGCAAAATTTTTGGAGCTACATCATGTGCAGAAATTAGAGATACCGTTTATAGCTATGTACCGAAAGGAGCAATGCCGGAGCTTGTTGGACACTGGTGATTTTGATGGCGCCAATCAAGGCAAGAAACCTGAGACCAAGTGGCATAAGGTCTTCTGGATGATTCATGACTTGGATAAGAAGTGGCTCCTTCTTCGAAAAAGGAAAATGGCGTTGCATGGTTACTATACAAAACGTTACGAAGAAGAGTCTAGGAGGGTCTATGATGAAACAAGACTTAATCTAAATCAGTATCTTTTTGAATCAGTCATTAAGTCTCTTAAAGTTGCAGAAACAGAAAGAGAAGTTGATGATGTAGATTCCAAGTTCAACTTGCATTTTCCTCCCGGTGAAATTGGTGTTGATGAAGGGCAGTATAAGAGGCCGAAAAGGAAATCACAATATAGCATCTGCAGTAAAGCAGGGCTCTGGGAGGTTGCAAACAAATTTGGGTATAGTGCTGAGCAACTGGGACTTGCATTGTCCCTAGAAAAACTGGTTGATGAGCTTGAGGATGCAAAGGAAACACCAGAGGAGATGGCTAAAAACTTTGTGTGCGCAATGTTTGAAAATTCTCTGGCAGTTCTGAAAGGTGCACGGCATATGGCTGCAGTTGAGATAAGTTGCGAGCCATCAGTCAAAAAGTATGTCCGTGGCATTTATATGGAGAATGCAGTGGTCTCAACAAGTCCAACAGCAGATGGAAATACAGTAATTGATTCGTTCCATCAGTTTTCTGGGATTAAGTGGTTGCGTGAAAAGCCATTGAGCAAGTTTGAGGGTGCACAATGGCTTCTCATTCAGAAGGGAGAAGAGGAGAAACTTCTTCAAGTAACCTTCAAGCTGCCTGAGAATTACATGAATAGGCTAATTAGCGACTGCAATGAACACTATCTAAGTGTTGGTGTTAGTAAGTATGCTCAACTCTGGAATGAGCAAAGAAAGTTGATATTGGAGGATGCACTTCATGCTTTTCTTTTGCCATCAATGGAGAAAGAAGCAAGATCCTTGCTAACTAGCAGAGCGAAGAGTCGGTTGCTTTCAGAGTATGGACAGGCTTTGTGGAACAAGGTGTCTGCAGGGCCATACCAAAAGAAAGAAATGGACATTAATTTAGATGAGGAAGCTGCACCGAGGGTCATGGCATGTTGTTGGGGACCTGGGAAGCCACCAAATACATTTGTGATGCTGGACTCATCAGGTGAGGTGCTTGACGTGCTTTATGCTGGATCCCTTACATCGCGATCTCAAAATGTTAACGACCAGCAACGTAAAAAGAGTGACCAGGATCGTGTTTTAAAGTTTATGATGGACCACCAACCGCATGTTGTGGCTTTAGGAGCTGTCAATTTGTCTTGTACTCGTCTGAAGGATGATATTTATGAGGTCATATTCCAGATGGTGGAGGAAAAACCTAGAGATGTCGGACATGGGATGGATGATTTAAGCATTGTTTATGTGGATGAATCACTTCCTAGGCTATATGAAAATTCTCGAATCTCGGGTGAACAGCTACCTCAGCAGTCAGGGAACGTGAGACGTGCAGTTGCTCTTGGACGCTATCTCCAGAATCCACTAGCAATGGTGGCAACTTTATGCGGTCCAGGTCGGGAAATTTTGTCTTGGAAGCTTCATCCCTTGGAGAATTTTCTTCAGCTAGATGAGAAGTATGGAATGGTTGAGCAGGTTATGGTTGACATTACAAACCAGGTTGGAATCGATATTAACTTGGCAGCTAGCCATGATTGGTTCTTTTCTCCTTTACAGTTCATTTCTGGACTTGGGCCTCGGAAAGCTGCATCCTTGCAGAGGTCGCTTGTAAGAGCTGGTTCAATCTTTGTCCGCAAGGACCTGATAATGCATGGACTTGGTAAAAAGGTTTTTGTAAATGCTGCCGGTTTCTTGCGCATCCGCAGAAGTGGGCTGGCTGCTAGTAGCAGTCAATTTATTGACTTATTGGATGACACCAGAATCCATCCAGAGTCGTATAGTCTTGCTCAAGAATTGGCGAAAGATATATATGATGAGGATGTCAGAGGTGATTCAAATGATGATGAGGATGCGATAGAAATGGCTATTGAGCATGTGAGAGATCGGCCAGCTTCTTTGAGAAAAGTTGTCCTTGATGAATATCTTGCCAGCAAGAAACGGGAGAATAAGAAGGAAACTTATAGCAATATCATAAGAGAATTAAGTTGTGGTTTCCAGGATTGGCGGATACCATTTAAAGAGCCAAGTCCAGATGAAGAATTTTACATGATTTCAGGTGAAACTGAGGACACCATAGCGGAGGGCAGAATTGTCCAGGCCAGTGTTCGGAGATTGCAGAATGGAAGAGCCATATGTGTATTAGATTCTGGGTTAACTGGGATGCTTATGAAGGAAGATTTCTCAGATGATGGGAGAGATATTGTTGACTTGGCGGACCAGCTGAAAGAAGGCGATATCCTCACATGCAAGATCAAATCGATTCAAAAGCAGAGGTATCAAGTGTTCCTTATTTGCAAAGAAAGTGAAATGAGAAACAACAGACACCAGCATAACCAGAATGTGGATGCATATTACCATGAGGATAGAAACAGTCTCCAGCTTGTAAAAGAGAAGGCTCGAAAGGAAAAAGAGCTGGTGAGGAAGCATTTTAAGTCTCGGATGATTGTCCATCCCCGCTTTCAGAATATCACAGCTGACCAAGCAACAGAGTATTTGTCTGACAAAGACTTTGGAGAAAGCATTGTTCGTCCAAGTTCTCGAGGACTCAACTTTTTAACTTTGACCCTCAAGATTTATGATGGAGTTTACGCTCACAAGGAGATAGCTGAAGGTGGGAAAGAAAACAAGGACATTACAAGCCTACAGTGTATTGGGAAGACCCTGACAATTGGAGAGGACACCTTCGAGGATCTAGATGAGGTCATGGATCGATATGTTGATCCTTTGGTATCTCATCTTAAGACAATGCTCAACTATCGGAAATTCCGTAAGGGGACCAAATCAGAAGTAGATGATCTTCTTAGGATCGAGAAGGGCGAAAATCCTTCAAGAATAGTTTATTGTTTTGGGATTTCACACGAACATCCAGGCACTTTTATTCTGTCTTACATAAGGAGCACAAACCCACATCATGAGTATATAGGTTTATATCCCAAGGGATTTAAGTTCAGGAAAAGGATGTTTGAGGACATTGATCGGCTTGTGGCATACTTCCAGAGGCATATTGATGACCCATTGCAAGAATCGGCGCCATCAATCAGGTCTATTGCTGCCAAGGTACCGATGAGAAGCCCAGCAGACCATGGATCATCTGGAGGAAGTGGTTGGGGTAGTTCTCAGAGTGAAGGAGGCTGGAAAGGTAACTCGGATCGTTCAGGCTCAGGGAGAGGCGGTGAGTACAGAAATGGTGGTGGACGTGATGGACATCCAAGTGGAGCGCCTAGGCCATATGGTGGCCGTGGACGTGGTCGAGGCAGAGGACGGAGGGATGATATGAATAGTGATAGACAAGATGGTAACGGAGATTGGGGTAATAATGATACTGGAACTGCAGATGGAGGTTGGGGAAATAGTGGTGGCGGTGGTTGGGGAAGCGAGTCTGCTGGTAAAAAGACTGGTGGTGGCAGTACTGGTGGTTGGGGAAGCGAGTCTGGTGGCAATAAGAGTGACGGTGCGGGCAGCTGGGGGAGTGGGTCTGGCGGTGGTGGTAGTGGAGGTTGGGGAAACGATTCTGGCGGTAAAAAGAGCAGTGAGGATGGCGGTTTTGGAAGTGGTTCTGGCGGTGGCGGAAGCGATTGGGGAAACGAGTCTGGTGGTAAAAAGAGCAGTGCAGATGGCGGTTGGGGAAGCGAGTCTGGTGGTAAAAAGAGCGATGGAGAGGGCGGTTGGGGGAATGAACCCAGCAGCCGGAAGAGCGATGGCGGTGGTGGTGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

1647

Amino Acids

185.03

Weight (kDa)

5.08

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT6_acidic PF14632 31 - 118 3.3e-13 Acidic N-terminal SPT6
HTH_44 PF14641 290 - 402 2.5e-06 Helix-turn-helix DNA-binding domain of SPT6
Tex_central_region PF22706 510 - 681 6.8e-08 Tex central region-like
YqgF PF14639 715 - 871 2.6e-16 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 875 - 975 8.1e-21 Helix-hairpin-helix motif
Spt6_S1 PF21710 1089 - 1174 9.5e-08 Spt6, S1/OB domain
SH2_2 PF14633 1192 - 1409 4.7e-70 SH2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1573, 2921
Acc36I ACCTGC 1 cut(s) 2689
Acc65I GGTACC 1 cut(s) 4282
AccB1I GGYRCC 3 cut(s) 1085, 4252, 4282
AccB7I CCANNNNNTGG 4 cut(s) 71, 2416, 3565, 4457
AccII CGCG 1 cut(s) 2257
AclI AACGTT 1 cut(s) 1210
AcoI YGGCCR 3 cut(s) 364, 3110, 4459
AcsI RAATTY 9 cut(s) 308, 983, 1460, 1585, 2503, 2628, 2659, 3263, 3986
AcuI CTGAAG 4 cut(s) 855, 2402, 2651, 3852
AcyI GRCGYC 2 cut(s) 1086, 4253
AfaI GTAC 5 cut(s) 1037, 2374, 4284, 4403, 4633
AfiI CCNNNNNNNGG 8 cut(s) 71, 590, 2416, 2823, 3330, 3565, 4457, 4911
AflII CTTAAG 1 cut(s) 3962
AjiI CACGTC 4 cut(s) 2230, 2555, 4421, 4469
AjnI CCWGG 8 cut(s) 366, 2173, 2298, 2617, 2721, 3219, 3318, 4090
AjuI GAANNNNNNNTTGG 4 cut(s) 507, 539, 745, 777
AloI GAACNNNNNNTCC 2 cut(s) 51, 83
Alw21I GWGCWC 3 cut(s) 1612, 1801, 4124
Alw26I GTCTC 6 cut(s) 1103, 1296, 1699, 2545, 3602, 3663
Alw44I GTGCAC 2 cut(s) 1608, 1797
Ama87I CYCGRG 2 cut(s) 2515, 3762
AoxI GGCC 8 cut(s) 364, 1397, 2096, 2798, 3110, 3321, 4448, 4459
ApaLI GTGCAC 2 cut(s) 1608, 1797
ApoI RAATTY 9 cut(s) 308, 983, 1460, 1585, 2503, 2628, 2659, 3263, 3986
ArsI GACNNNNNNTTYG 6 cut(s) 431, 463, 1647, 1679, 3716, 3748
AseI ATTAAT 1 cut(s) 2124
Asp700I GAANNNNTTC 3 cut(s) 2484, 4732, 4777
Asp718I GGTACC 1 cut(s) 4282
AspA2I CCTAGG 2 cut(s) 2489, 4445
AspLEI GCGC 5 cut(s) 1088, 1574, 2922, 4255, 4444
AspS9I GGNCC 9 cut(s) 578, 2096, 2171, 2326, 2615, 2798, 2863, 3439, 3999
AsuC2I CCSGG 1 cut(s) 1362
AsuII TTCGAA 1 cut(s) 1171
AsuNHI GCTAGC 1 cut(s) 2750
AvaI CYCGRG 2 cut(s) 2515, 3762
AvaII GGWCC 7 cut(s) 578, 2171, 2326, 2615, 2863, 3439, 3999
AvrII CCTAGG 2 cut(s) 2489, 4445
BaeGI GKGCMC 2 cut(s) 1612, 1801
BalI TGGCCA 1 cut(s) 366
BamHI GGATCC 1 cut(s) 2243
BanI GGYRCC 3 cut(s) 1085, 4252, 4282
BanII GRGCYC 1 cut(s) 1443
BarI GAAGNNNNNNTAC 2 cut(s) 4005, 4037
BbsI GAAGAC 5 cut(s) 174, 198, 504, 1120, 3887
Bbv12I GWGCWC 3 cut(s) 1612, 1801, 4124
BbvCI CCTCAGC 2 cut(s) 423, 2532
BceAI ACGGC 2 cut(s) 1628, 4446
BciT130I CCWGG 8 cut(s) 368, 2175, 2300, 2619, 2723, 3221, 3320, 4092
BciVI GTATCC 1 cut(s) 3225
BcnI CCSGG 1 cut(s) 1362
BcoDI GTCTC 6 cut(s) 1103, 1296, 1699, 2545, 3602, 3663
BfmI CTRYAG 6 cut(s) 858, 1426, 1623, 2091, 3866, 4554
BfoI RGCGCY 3 cut(s) 1089, 4256, 4445
BfrI CTTAAG 1 cut(s) 3962
BfuAI ACCTGC 1 cut(s) 2689
BfuI GTATCC 1 cut(s) 3225
BlnI CCTAGG 2 cut(s) 2489, 4445
BlpI GCTNAGC 2 cut(s) 84, 1476
BmcAI AGTACT 1 cut(s) 4633
Bme1390I CCNGG 9 cut(s) 368, 1362, 2175, 2300, 2619, 2723, 3221, 3320, 4092
Bme18I GGWCC 7 cut(s) 578, 2171, 2326, 2615, 2863, 3439, 3999
BmeT110I CYCGRG 2 cut(s) 2515, 3762
BmgBI CACGTC 4 cut(s) 2230, 2555, 4421, 4469
BmgT120I GGNCC 9 cut(s) 578, 2096, 2171, 2326, 2615, 2798, 2863, 3439, 3999
BmiI GGNNCC 7 cut(s) 1087, 1163, 2172, 2245, 4000, 4254, 4284
BmrFI CCNGG 9 cut(s) 368, 1362, 2175, 2300, 2619, 2723, 3221, 3320, 4092
BmrI ACTGGG 2 cut(s) 1493, 3390
BmtI GCTAGC 1 cut(s) 2754
BmuI ACTGGG 2 cut(s) 1493, 3390
BoxI GACNNNNGTC 1 cut(s) 261
BpiI GAAGAC 5 cut(s) 174, 198, 504, 1120, 3887
BpmI CTGGAG 3 cut(s) 2564, 3584, 4338
Bpu10I CCTNAGC 3 cut(s) 423, 2532, 4384
Bpu1102I GCTNAGC 2 cut(s) 84, 1476
Bpu14I TTCGAA 1 cut(s) 1171
BpuEI CTTGAG 4 cut(s) 65, 1544, 2997, 3776
BpuMI CCSGG 1 cut(s) 1362
Bsa29I ATCGAT 3 cut(s) 2733, 3482, 3934
BsaBI GATNNNNATC 2 cut(s) 3144, 4428
BsaHI GRCGYC 2 cut(s) 1086, 4253
BsaI GGTCTC 2 cut(s) 1103, 1699
BsaWI WCCGGW 1 cut(s) 845
BsaXI ACNNNNNCTCC 6 cut(s) 51, 81, 2562, 2592, 4381, 4411
Bsc4I CCNNNNNNNGG 8 cut(s) 71, 590, 2416, 2823, 3330, 3565, 4457, 4911
Bse118I RCCGGY 1 cut(s) 2909
Bse1I ACTGG 9 cut(s) 65, 1075, 1488, 1515, 3324, 3385, 4552, 4624, 4639
Bse3DI GCAATG 5 cut(s) 1055, 1581, 1903, 2601, 4238
Bse8I GATNNNNATC 2 cut(s) 3144, 4428
BseBI CCWGG 8 cut(s) 368, 2175, 2300, 2619, 2723, 3221, 3320, 4092
BseCI ATCGAT 3 cut(s) 2733, 3482, 3934
BseJI GATNNNNATC 2 cut(s) 3144, 4428
BseLI CCNNNNNNNGG 8 cut(s) 71, 590, 2416, 2823, 3330, 3565, 4457, 4911
BseMI GCAATG 5 cut(s) 1055, 1581, 1903, 2601, 4238
BseNI ACTGG 9 cut(s) 65, 1075, 1488, 1515, 3324, 3385, 4552, 4624, 4639
BseRI GAGGAG 3 cut(s) 233, 1562, 1844
BseSI GKGCMC 2 cut(s) 1612, 1801
BseYI CCCAGC 3 cut(s) 4297, 4686, 4904
BsgI GTGCAG 4 cut(s) 1025, 2127, 2577, 4854
Bsh1236I CGCG 1 cut(s) 2257
BshFI GGCC 8 cut(s) 366, 1399, 2098, 2800, 3112, 3323, 4450, 4461
BshNI GGYRCC 3 cut(s) 1085, 4252, 4282
BshVI ATCGAT 3 cut(s) 2733, 3482, 3934
BsiHKAI GWGCWC 3 cut(s) 1612, 1801, 4124
BsiHKCI CYCGRG 2 cut(s) 2515, 3762
BsiSI CCGG 5 cut(s) 846, 1054, 1362, 2910, 4912
BslFI GGGAC 4 cut(s) 1483, 1500, 2184, 4012
BslI CCNNNNNNNGG 8 cut(s) 71, 590, 2416, 2823, 3330, 3565, 4457, 4911
BsmAI GTCTC 6 cut(s) 1103, 1296, 1699, 2545, 3602, 3663
BsmBI CGTCTC 1 cut(s) 2545
BsmFI GGGAC 4 cut(s) 1483, 1500, 2184, 4012
BsmI GAATGC 2 cut(s) 828, 1690
BsnI GGCC 8 cut(s) 366, 1399, 2098, 2800, 3112, 3323, 4450, 4461
Bso31I GGTCTC 2 cut(s) 1103, 1699
BsoBI CYCGRG 2 cut(s) 2515, 3762
Bsp119I TTCGAA 1 cut(s) 1171
Bsp1286I GDGCHC 4 cut(s) 1443, 1612, 1801, 4124
Bsp1720I GCTNAGC 2 cut(s) 84, 1476
Bsp19I CCATGG 1 cut(s) 4306
Bsp68I TCGCGA 1 cut(s) 2257
BspANI GGCC 8 cut(s) 366, 1399, 2098, 2800, 3112, 3323, 4450, 4461
BspDI ATCGAT 3 cut(s) 2733, 3482, 3934
BspFNI CGCG 1 cut(s) 2257
BspHI TCATGA 3 cut(s) 37, 1141, 4135
BspLI GGNNCC 7 cut(s) 1087, 1163, 2172, 2245, 4000, 4254, 4284
BspMAI CTGCAG 4 cut(s) 1430, 1627, 2095, 4558
BspMI ACCTGC 1 cut(s) 2689
BspOI GCTAGC 1 cut(s) 2754
BspQI GCTCTTC 2 cut(s) 3343, 4910
BspT104I TTCGAA 1 cut(s) 1171
BspT107I GGYRCC 3 cut(s) 1085, 4252, 4282
BspTI CTTAAG 1 cut(s) 3962
BspTNI GGTCTC 2 cut(s) 1103, 1699
BsrDI GCAATG 5 cut(s) 1055, 1581, 1903, 2601, 4238
BsrFI RCCGGY 1 cut(s) 2909
BsrI ACTGG 9 cut(s) 65, 1075, 1488, 1515, 3324, 3385, 4552, 4624, 4639
BssAI RCCGGY 1 cut(s) 2909
BssNI GRCGYC 2 cut(s) 1086, 4253
BssT1I CCWWGG 6 cut(s) 2489, 2652, 4158, 4278, 4306, 4445
Bst2UI CCWGG 8 cut(s) 368, 2175, 2300, 2619, 2723, 3221, 3320, 4092
Bst4CI ACNGT 7 cut(s) 757, 1023, 1726, 2781, 3596, 3870, 4676
Bst6I CTCTTC 8 cut(s) 24, 168, 177, 1215, 1821, 2036, 3343, 4910
BstACI GRCGYC 2 cut(s) 1086, 4253
BstAFI CTTAAG 1 cut(s) 3962
BstAPI GCANNNNNTGC 1 cut(s) 1982
BstBI TTCGAA 1 cut(s) 1171
BstC8I GCNNGC 7 cut(s) 803, 1643, 2752, 2940, 3114, 3154, 4681
BstDSI CCRYGG 3 cut(s) 1666, 4306, 4462
BstENI CCTNNNNNAGG 1 cut(s) 2821
BstFNI CGCG 1 cut(s) 2257
BstH2I RGCGCY 3 cut(s) 1089, 4256, 4445
BstHHI GCGC 5 cut(s) 1088, 1574, 2922, 4255, 4444
BstMAI GTCTC 6 cut(s) 1103, 1296, 1699, 2545, 3602, 3663
BstNI CCWGG 8 cut(s) 368, 2175, 2300, 2619, 2723, 3221, 3320, 4092
BstNSI RCATGY 4 cut(s) 2163, 2342, 3101, 3471
BstPAI GACNNNNGTC 1 cut(s) 261
BstSCI CCNGG 9 cut(s) 366, 1360, 2173, 2298, 2617, 2721, 3219, 3318, 4090
BstSFI CTRYAG 6 cut(s) 858, 1426, 1623, 2091, 3866, 4554
BstSLI GKGCMC 2 cut(s) 1612, 1801
BstUI CGCG 1 cut(s) 2257
BstV2I GAAGAC 5 cut(s) 174, 198, 504, 1120, 3887
BstX2I RGATCY 4 cut(s) 76, 2018, 2243, 3913
BstYI RGATCY 4 cut(s) 76, 2018, 2243, 3913
Bsu15I ATCGAT 3 cut(s) 2733, 3482, 3934
BsuI GTATCC 1 cut(s) 3225
BsuRI GGCC 8 cut(s) 366, 1399, 2098, 2800, 3112, 3323, 4450, 4461
BsuTUI ATCGAT 3 cut(s) 2733, 3482, 3934
BtgI CCRYGG 3 cut(s) 1666, 4306, 4462
BtgZI GCGATG 5 cut(s) 228, 961, 2238, 4890, 4935
BtrI CACGTC 4 cut(s) 2230, 2555, 4421, 4469
BtsI GCAGTG 3 cut(s) 1695, 4759, 4837
BtsIMutI CAGTG 6 cut(s) 1068, 1695, 3331, 3875, 4759, 4837
BtuMI TCGCGA 1 cut(s) 2257
BveI ACCTGC 1 cut(s) 2689
Cac8I GCNNGC 7 cut(s) 803, 1643, 2752, 2940, 3114, 3154, 4681
CciI TCATGA 3 cut(s) 37, 1141, 4135
CfoI GCGC 5 cut(s) 1088, 1574, 2922, 4255, 4444
Cfr10I RCCGGY 1 cut(s) 2909
Cfr13I GGNCC 9 cut(s) 578, 2096, 2171, 2326, 2615, 2798, 2863, 3439, 3999
ClaI ATCGAT 3 cut(s) 2733, 3482, 3934
CseI GACGC 1 cut(s) 2580
CsiI ACCWGGT 1 cut(s) 2721
Csp6I GTAC 5 cut(s) 1036, 2373, 4283, 4402, 4632
CviQI GTAC 5 cut(s) 1036, 2373, 4283, 4402, 4632
DinI GGCGCC 2 cut(s) 1087, 4254
DraI TTTAAA 2 cut(s) 2313, 3241
EaeI YGGCCR 3 cut(s) 364, 3110, 4459
Eam1104I CTCTTC 8 cut(s) 24, 168, 177, 1215, 1821, 2036, 3343, 4910
EarI CTCTTC 8 cut(s) 24, 168, 177, 1215, 1821, 2036, 3343, 4910
EciI GGCGGA 4 cut(s) 788, 3244, 3452, 4806
Eco130I CCWWGG 6 cut(s) 2489, 2652, 4158, 4278, 4306, 4445
Eco24I GRGCYC 1 cut(s) 1443
Eco31I GGTCTC 2 cut(s) 1103, 1699
Eco32I GATATC 2 cut(s) 831, 3460
Eco47I GGWCC 7 cut(s) 578, 2171, 2326, 2615, 2863, 3439, 3999
Eco57I CTGAAG 4 cut(s) 855, 2402, 2651, 3852
Eco88I CYCGRG 2 cut(s) 2515, 3762
EcoNI CCTNNNNNAGG 1 cut(s) 2821
EcoO109I RGGNCCY 2 cut(s) 2171, 2863
EcoRII CCWGG 8 cut(s) 366, 2173, 2298, 2617, 2721, 3219, 3318, 4090
EcoRV GATATC 2 cut(s) 831, 3460
EcoT14I CCWWGG 6 cut(s) 2489, 2652, 4158, 4278, 4306, 4445
EcoT22I ATGCAT 2 cut(s) 2877, 3574
EcoT38I GRGCYC 1 cut(s) 1443
EgeI GGCGCC 2 cut(s) 1087, 4254
EheI GGCGCC 2 cut(s) 1087, 4254
ErhI CCWWGG 6 cut(s) 2489, 2652, 4158, 4278, 4306, 4445
Esp3I CGTCTC 1 cut(s) 2545
FalI AAGNNNNNCTT 6 cut(s) 1331, 1363, 3120, 3152, 3495, 3527
FaqI GGGAC 4 cut(s) 1483, 1500, 2184, 4012
FauI CCCGC 3 cut(s) 942, 3686, 4672
FauNDI CATATG 3 cut(s) 1617, 3356, 4453
FriOI GRGCYC 1 cut(s) 1443
FspI TGCGCA 2 cut(s) 1573, 2921
GlaI GCGC 5 cut(s) 1087, 1573, 2921, 4254, 4443
GsaI CCCAGC 3 cut(s) 4301, 4690, 4908
GsuI CTGGAG 3 cut(s) 2564, 3584, 4338
HaeII RGCGCY 3 cut(s) 1089, 4256, 4445
HaeIII GGCC 8 cut(s) 366, 1399, 2098, 2800, 3112, 3323, 4450, 4461
HapII CCGG 5 cut(s) 846, 1054, 1362, 2910, 4912
HgaI GACGC 1 cut(s) 2580
HhaI GCGC 5 cut(s) 1088, 1574, 2922, 4255, 4444
Hin1I GRCGYC 2 cut(s) 1086, 4253
Hin6I GCGC 5 cut(s) 1086, 1572, 2920, 4253, 4442
HinP1I GCGC 5 cut(s) 1086, 1572, 2920, 4253, 4442
HincII GTYRAC 5 cut(s) 673, 2272, 2710, 3378, 3430
HindII GTYRAC 5 cut(s) 673, 2272, 2710, 3378, 3430
HindIII AAGCTT 1 cut(s) 2642
HpaI GTTAAC 2 cut(s) 2272, 3378
HpaII CCGG 5 cut(s) 846, 1054, 1362, 2910, 4912
Hpy99I CGWCG 1 cut(s) 59
HpyCH4III ACNGT 7 cut(s) 757, 1023, 1726, 2781, 3596, 3870, 4676
HpyCH4IV ACGT 9 cut(s) 123, 303, 1210, 2229, 2284, 2547, 2554, 4420, 4468
HpySE526I ACGT 9 cut(s) 123, 303, 1210, 2229, 2284, 2547, 2554, 4420, 4468
Hsp92I GRCGYC 2 cut(s) 1086, 4253
HspAI GCGC 5 cut(s) 1086, 1572, 2920, 4253, 4442
KasI GGCGCC 2 cut(s) 1085, 4252
KpnI GGTACC 1 cut(s) 4286
KspAI GTTAAC 2 cut(s) 2272, 3378
LguI GCTCTTC 2 cut(s) 3343, 4910
LmnI GCTCC 8 cut(s) 528, 991, 1045, 1056, 1167, 2354, 4119, 4439
MabI ACCWGGT 1 cut(s) 2721
MaeII ACGT 9 cut(s) 123, 303, 1210, 2229, 2284, 2547, 2554, 4420, 4468
MaeIII GTNAC 7 cut(s) 1196, 1211, 1846, 2294, 4364, 4520, 4670
MfeI CAATTG 2 cut(s) 889, 3891
MflI RGATCY 4 cut(s) 76, 2018, 2243, 3913
MhlI GDGCHC 4 cut(s) 1443, 1612, 1801, 4124
MlsI TGGCCA 1 cut(s) 366
MluNI TGGCCA 1 cut(s) 366
Mly113I GGCGCC 2 cut(s) 1086, 4253
MlyI GAGTC 9 cut(s) 1232, 2053, 2201, 3005, 3762, 4610, 4661, 4820, 4865
MmeI TCCRAC 4 cut(s) 1044, 1730, 2419, 2707
Mox20I TGGCCA 1 cut(s) 366
Mph1103I ATGCAT 2 cut(s) 2877, 3574
MroXI GAANNNNTTC 3 cut(s) 2484, 4732, 4777
MscI TGGCCA 1 cut(s) 366
MslI CAYNNNNRTG 3 cut(s) 1676, 4230, 4434
Msp20I TGGCCA 1 cut(s) 366
MspA1I CMGCKG 4 cut(s) 83, 3445, 3698, 4686
MspCI CTTAAG 1 cut(s) 3962
MspI CCGG 5 cut(s) 846, 1054, 1362, 2910, 4912
MspR9I CCNGG 9 cut(s) 368, 1362, 2175, 2300, 2619, 2723, 3221, 3320, 4092
MunI CAATTG 2 cut(s) 889, 3891
Mva1269I GAATGC 2 cut(s) 828, 1690
MvaI CCWGG 8 cut(s) 368, 2175, 2300, 2619, 2723, 3221, 3320, 4092
MvnI CGCG 1 cut(s) 2257
NarI GGCGCC 2 cut(s) 1086, 4253
NciI CCSGG 1 cut(s) 1362
NcoI CCATGG 1 cut(s) 4306
NdeI CATATG 3 cut(s) 1617, 3356, 4453
NheI GCTAGC 1 cut(s) 2750
NlaIV GGNNCC 7 cut(s) 1087, 1163, 2172, 2245, 4000, 4254, 4284
NmuCI GTSAC 2 cut(s) 2294, 4670
NruI TCGCGA 1 cut(s) 2257
NsbI TGCGCA 2 cut(s) 1573, 2921
NsiI ATGCAT 2 cut(s) 2877, 3574
NspI RCATGY 4 cut(s) 2163, 2342, 3101, 3471
NspV TTCGAA 1 cut(s) 1171
PaeR7I CTCGAG 1 cut(s) 3762
PagI TCATGA 3 cut(s) 37, 1141, 4135
PciSI GCTCTTC 2 cut(s) 3343, 4910
PcsI WCGNNNNNNNCGW 2 cut(s) 3988, 4038
PctI GAATGC 2 cut(s) 828, 1690
PdmI GAANNNNTTC 3 cut(s) 2484, 4732, 4777
PflFI GACNNNGTC 1 cut(s) 4470
PflMI CCANNNNNTGG 4 cut(s) 71, 2416, 3565, 4457
PfoI TCCNGGA 1 cut(s) 3219
PleI GAGTC 9 cut(s) 1231, 2052, 2201, 3004, 3762, 4609, 4660, 4819, 4864
PluTI GGCGCC 2 cut(s) 1089, 4256
PpsI GAGTC 9 cut(s) 1231, 2052, 2201, 3004, 3762, 4609, 4660, 4819, 4864
PpuMI RGGWCCY 2 cut(s) 2171, 2863
PshAI GACNNNNGTC 1 cut(s) 261
PshBI ATTAAT 1 cut(s) 2124
Psp1406I AACGTT 1 cut(s) 1210
Psp5II RGGWCCY 2 cut(s) 2171, 2863
Psp6I CCWGG 8 cut(s) 366, 2173, 2298, 2617, 2721, 3219, 3318, 4090
PspFI CCCAGC 3 cut(s) 4297, 4686, 4904
PspGI CCWGG 8 cut(s) 366, 2173, 2298, 2617, 2721, 3219, 3318, 4090
PspN4I GGNNCC 7 cut(s) 1087, 1163, 2172, 2245, 4000, 4254, 4284
PspPI GGNCC 9 cut(s) 578, 2096, 2171, 2326, 2615, 2798, 2863, 3439, 3999
PspPPI RGGWCCY 2 cut(s) 2171, 2863
PsrI GAACNNNNNNTAC 2 cut(s) 3491, 3523
PstI CTGCAG 4 cut(s) 1430, 1627, 2095, 4558
PsuI RGATCY 4 cut(s) 76, 2018, 2243, 3913
PsyI GACNNNGTC 1 cut(s) 4470
PvuII CAGCTG 4 cut(s) 83, 3445, 3698, 4686
RruI TCGCGA 1 cut(s) 2257
RsaI GTAC 5 cut(s) 1037, 2374, 4284, 4403, 4633
RsaNI GTAC 5 cut(s) 1036, 2373, 4283, 4402, 4632
RseI CAYNNNNRTG 3 cut(s) 1676, 4230, 4434
SapI GCTCTTC 2 cut(s) 3343, 4910
Sau96I GGNCC 9 cut(s) 578, 2096, 2171, 2326, 2615, 2798, 2863, 3439, 3999
ScaI AGTACT 1 cut(s) 4633
SchI GAGTC 9 cut(s) 1232, 2053, 2201, 3005, 3762, 4610, 4661, 4820, 4865
ScrFI CCNGG 9 cut(s) 368, 1362, 2175, 2300, 2619, 2723, 3221, 3320, 4092
SduI GDGCHC 4 cut(s) 1443, 1612, 1801, 4124
SexAI ACCWGGT 1 cut(s) 2721
SfcI CTRYAG 6 cut(s) 858, 1426, 1623, 2091, 3866, 4554
SfoI GGCGCC 2 cut(s) 1087, 4254
Sfr274I CTCGAG 1 cut(s) 3762
SfuI TTCGAA 1 cut(s) 1171
SinI GGWCC 7 cut(s) 578, 2171, 2326, 2615, 2863, 3439, 3999
SlaI CTCGAG 1 cut(s) 3762
SmiMI CAYNNNNRTG 3 cut(s) 1676, 4230, 4434
SmlI CTYRAG 6 cut(s) 44, 1523, 3012, 3762, 3791, 3962
SmoI CTYRAG 6 cut(s) 44, 1523, 3012, 3762, 3791, 3962
SspDI GGCGCC 2 cut(s) 1085, 4252
SspI AATATT 1 cut(s) 660
StyD4I CCNGG 9 cut(s) 366, 1360, 2173, 2298, 2617, 2721, 3219, 3318, 4090
StyI CCWWGG 6 cut(s) 2489, 2652, 4158, 4278, 4306, 4445
TaaI ACNGT 7 cut(s) 757, 1023, 1726, 2781, 3596, 3870, 4676
TaiI ACGT 9 cut(s) 126, 306, 1213, 2232, 2287, 2550, 2557, 4423, 4471
TatI WGTACW 3 cut(s) 2372, 4401, 4631
TscAI CASTG 6 cut(s) 1075, 1695, 3331, 3875, 4759, 4837
TseFI GTSAC 2 cut(s) 2294, 4670
Tsp45I GTSAC 2 cut(s) 2294, 4670
TspGWI ACGGA 5 cut(s) 72, 1655, 3980, 4501, 4539
TspRI CASTG 6 cut(s) 1075, 1695, 3331, 3875, 4759, 4837
Tth111I GACNNNGTC 1 cut(s) 4470
Van91I CCANNNNNTGG 4 cut(s) 71, 2416, 3565, 4457
Vha464I CTTAAG 1 cut(s) 3962
VneI GTGCAC 2 cut(s) 1608, 1797
VpaK11BI GGWCC 7 cut(s) 578, 2171, 2326, 2615, 2863, 3439, 3999
VspI ATTAAT 1 cut(s) 2124
XagI CCTNNNNNAGG 1 cut(s) 2821
XapI RAATTY 9 cut(s) 308, 983, 1460, 1585, 2503, 2628, 2659, 3263, 3986
XbaI TCTAGA 1 cut(s) 3916
XceI RCATGY 4 cut(s) 2163, 2342, 3101, 3471
XcmI CCANNNNNNNNNTGG 2 cut(s) 1748, 4313
XhoI CTCGAG 1 cut(s) 3762
XmaJI CCTAGG 2 cut(s) 2489, 4445
XmnI GAANNNNTTC 3 cut(s) 2484, 4732, 4777
ZrmI AGTACT 1 cut(s) 4633
Zsp2I ATGCAT 2 cut(s) 2877, 3574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.