Rh3CG348100

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0009494
Physical Location & Seq
Forward (+)
41475053 .. 41482039
6987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG348100.1

Sequence Viewer

Length: 4650 bp
ATGTGTGTGTTTGTGCATGATGAAGATTTCAAGGGTTTTCTCTTCTTTCTTTGGCCAAACTTTCAGGGGAAATTTAAGCGGCTGAAAAAAGCTCAGAGGCATGCTCAGGGGGACCTTGGTGGATTATCTGATGAGGAGGAGTTTCTTGGTACTGGTAAAGGGGGAAGAACTGCAGAGGAGAAGCTTAAGTTCTCATTATTTGGTGGTGATGATGAAGGCCCTCCACTTGAGGACATTGCTGAAGAGGAGGAACCAGCAGAAGCGGAAGATGACGGAGAAGATGAAATGGCAGACTTTATAGTCGACGAAGAATTTGATGAGGCTGGTGTTCCTGTCAGACAGAAGAAGCTTAAGAAAAAGAAATCCAGGCAGGCACCAGGAGTATCTTCATCTGCTCTGCAGGAAGCCCATGACATATTCGGTGATGTTGATGAATTACTGCAGCTGCGTAAGCAAGGTTTGGATTCTAGTGAATGGAAGGAAAGGAGGCTTGAAGATGAATTTGAACCAATTGTTCTTTCGGAAAAGTATATGACAGAGAAGGATGACCAGATTCGGGAGATTGATGTCCCAGAAAGAATTCAGGTATATGAGGAAAGCACTGGTTTTCCTCCACTTGATGAAAAGAGCATAGATGATGAGAGTACCTGGATATTCAATCAACTTGCAAGTGGTACAGTTCCTTTTTTTGGCAAGACAGGCCTGGGGAATTCCATAAGCAGGGACGACATCATTGGATTCTTGAACTTGCACCATGTGCAGAAATTGGATGTTCCTTTTATTGCGATGTATCGGAAAGAGGAGTGCCTTAGTATATTGAAAGATCCTGAACACATGGACATGGATGATCAAAATGAGAAAGCATCCACACTAAAGTGGCACAAGGTACTTTGGTCCATTCAGGACTTGCATAGAAAATGGTTGCTGCTTCAGAAGAGGAAAAGTGCTCTGCAATCTTATTACGAAAAGCGGTTTGATGAAGAATCTCGACGCATATATGATGAGACCAGACTAACTTTAAATCAGCAACTTTTTGAATCAATTATGAAGTCACTAAAGGCTGCAGAATCAGAGAGAGAAGTTGATGATGTGGACACCAAGTTTAACTTGCATTTCCCTGCTGGTGAGATAGGTGTGGATGAGGGCCAATATAAGAGGCCTAAGAGGAAGTCCCTTTATAGTATCTGCAGTAAGGCTGGCTTGTGGGAGGTTGCCAGCAAGTTTGGTTACACCTCTGAACAGTTTGGTTTGCAATTGTCACTAGAGAAAATGAGAATGGATGAGCTGGAGGATGCGAAAGAGACGCCAGAGGAATTGGCTTCAAACTTCACATGTGCAATGTTTGAGACACCTCAGGAAGTGCTTAAAGGAGCCAGGCATATGGCTGCTGTTGAGATAAGCTGTGAGCCATGTGTCAGGAAGTATGTTAGGAGCAACTACTTGGATATGGTTGAGTTGTCCACCAGCCCAACACCAGACGGAAATACTGCAATTGAAGCTTTTCATCAGTTTGCAGGGGTGAAGTGGTTGCAAAGAAAGCCATTAAATAGATTTGGGGATGCACAATGGCTGCTTATCCAGAAGGCTGAAGAGGAGAAGCTTCTTCAAGTTACTATTAAGCTACCGGAAGACAGACTAAACAAGTTGATGAGCGACTTTAATGAGTACTATCTTAGTGATGGTGTTAGTAAATCTGCACAACTGTGGAATGAGCAGCGGAAGTTGATATTGCAGGATGCTCTTTTCAATTTTCTTCTACCCTCAATGGAGAAAGAAGCAAGATCCTTGTTGACTAGTAGAGCAAAAAACTGGTTATTGATGGAATACGGAAAGGTTTTGTGGAATAAAGTCTCTGTGGGACCATATCAACGGAAAGAGAATGACATTAGTTCAGACGATGAAGCTGCACCCAGGGTCATGGCTTGCTGTTGGGGCCCTGGGAAGCCAGCAACCACTTTTGTTATGTTAGATTCATCGGGGGAGGTGCTAGATGTGCTTTACACGGGATCTCTCACCTTACGCTCCCAGAACGTTAACGATCAGCAACGGAAGAAAAATGACCAAGAACGTGTTTTGAAGTTCATGACTGATCACCAACCACATGTGGCTGTTCTAGGAGCTGTTAATTTGTCTTGTGTTCGCCTGAAAGAAGATATTTACGAGATAATCTTTAAGATGGTGGAGGAGAATCCTAGAGATGTTGGACATGATATGGATGGGTTAAGCATTGTCTATGGAGATGAATCTTTGGCTCGCCTGTATGAAAACTCTCGCATTTCATCTGATCAGCTACCTGCACAATCAGGCATTGTGAAGCGTGCTGTTGCTCTGGGGCGTTACCTTCAAAATCCGTTGGCAATGGTTGCCACTCTTTGTGGGCCAGGAAGAGAAATCTTATCTTGGAAACTCAATCCTTTGGAGAACTTTCTCACCCCAGATGAGAAGTATGCAATGATAGAACAAGTCATGGTAGATGTTACAAACCAAGTAGGCCTCGACATAAATTTGGCTATAAGCCACGAATGGTTATTTGCACCTTTGCAATTCATTTCTGGGCTCGGACCTAGAAAAGCAGCATTTTTGCAGCGATCGTTGGTAAGATCTGGTGCAATATTTACTCGGAAGGACTTTTTGACAGCACATGGGTTAAGTAAAAAGGTGTTTGTTAATGCAGTTGGTTTCTTGCGTGTTCGGCGTAGTGGGCTGGCTGCTAGCAGCAGCCAATTTATTGATCTGTTGGATGATACAAGAATTCATCCAGAATCCTATATTCTTGCACAAGAATTGGCCAAAGATGTTTTTGAAGTTGATGGTGGCAATGATGATGAGGATGCTATGGAGATGGCTATAGAGCATGTTAGGGATCGGCCTGCTTATCTAAAAAATCTTGATGTTGAAGCGTATGCTAAAAGCAAAGAGCGCGAGAATAAGATACAAACATTTTGTGACATCAGGAGAGAATTAATACAGGGTTTTCAGGATTGGCGTAAGAAATATGAAGAACTAAGTCAAGATGATGAATTCTATATGATTTCAGGTGAAACTGAAGATACCCTTGCTGAAGGAAGAATTGTGCTGGCCACAGTTCGCAGGGTACAAGCTCAAAGGGCAATATGTGCACTTGAATCTGGACTGACTGGTATGCTTATGAAAGAAGACTATTCAGATGATTCAAGGGATATTTCTGACTTATCTGAGAGACTGAATGAGGGTGACATTCTCACGTGCAAGATCAAATCTATTCAAAAGAATAGGTATCAGGTGTTCCTAGTTTGTAGAGAGAGTGAAATGAGAAATAATCGAAATCAAAATATTCAGCATCTCGATTCCTATTACCATGAAGACAAGAGAAGTCTACAGACAGAGCAAGAAAAAGCTCGTAAGGAGAAAGAGCTTGCGAAAAAACATTTCAAGCCACGGATGATTGTTCATCCTCGCTTCCAGAATATTACGGCTGATGAAGCAATGAAGTTCTTATCTGATAAAGATCCTGGGGAAAGTATTATTCGTCCTAGTTCCCGTGGGCCTTCGTATTTGACCTTAACTCTGAAAGTTTACGATGGAGTTTATGCTCACAAAGACGTAGTTGAAGGTGGAAAGGAACACAAGGATATCACTAGCTTACTCCGTATTGGAAAGACTCTGAAAATTGGAGAGGACACTTTTGAAGATTTAGATGAGGTAATGGATCGTTATGTTGATCCCCTGGTGTCTCACTTGAAGGCAATGCTAAATTATCGCAAATTCCGACGGGGCACAAAAGCAGAGGTTGATGAACTGTTGAAGATTGAAAAATTGGAGTTTCCTATGAGGATTGTTTATTGTTTCGGGATATCACATGAACATCCAGGGACTTTCATACTGACCTATATACGGAGTACAAATCCACACCATGAGTATGTTGGGCTGTATCCTAAGGGGTTCAAGTTCAGAAAAAGGATGTTTGAGAGCATTGATCGGCTTGTAGCACATTTTCAGAAGTATATTGATGAGCCTCAACATGAGTCCGGACCATCAATTAGATCAGTTGCTGCTATGGTGCCCATGCGAAGCCCTGCAACTGGGGGTTCTTCGGGTGCATCCGCAGGATGGGGTGGTTCAAACAATGAGGGTGGTTGGAGAGGCCAGTCGTTTGACAGGGATAGATCTTCTACACCGAGTTCGCGAACAGGTAGAAATGATTACAGAAATGGTGGTGGTCGAGATGGACATCCAAGTGGCCTACCTAGACCATATGGGGGGCGCGGTCGTGGTCGAGGAGCATATAACGATAGCCGAGGCAATAATGAGAGACATGATTCAGGTTTTGATGGTCCTACATGGGGTTCAGATACCAAGGATGGCAATGATGTCTTGGGCAACTTTCCAGGTGCAAAGGTTCAAAACTCACCTGGCAGGGAAGCTTTCCCAGGTGGCTGGGGTACAGGTGGAAGTGGTAGTGGAGGAGGCAGCAGCTGGGCTGGTGGTGGCGGAAGTGGCTGGGGTCAAGAAAGTGGTGGTGGCTCAGGAGGATGGGGTGGGGCCGCTGCAAATGATGCTGCTGCTGATAATGAGCCTTCAGGATGGGGTTCTGACTCGAAAAAGAAGGGTTCGGGTGATGGTACTTCAGGATGGGGTTCTCAGAGTGGAGGTGGCGGTGGTGGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

1549

Amino Acids

174.61

Weight (kDa)

5.43

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_44 PF14641 206 - 323 2.4e-06 Helix-turn-helix DNA-binding domain of SPT6
Tex_central_region PF22706 432 - 601 1.1e-06 Tex central region-like
YqgF PF14639 636 - 792 3.9e-17 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 796 - 897 9.9e-21 Helix-hairpin-helix motif
HHH_9 PF17674 912 - 996 9.8e-06 HHH domain
Spt6_S1 PF21710 1008 - 1096 1.3e-08 Spt6, S1/OB domain
SH2_2 PF14633 1112 - 1327 5.5e-69 SH2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
Acc36I ACCTGC 1 cut(s) 2302
AccB1I GGYRCC 2 cut(s) 373, 4039
AccI GTMKAC 2 cut(s) 303, 3355
AccII CGCG 3 cut(s) 2922, 4165, 4245
AccIII TCCGGA 1 cut(s) 4007
AciI CCGC 9 cut(s) 79, 263, 970, 1717, 4083, 4245, 4470, 4524, 4635
AclI AACGTT 1 cut(s) 2031
AclWI GGATC 7 cut(s) 818, 1776, 2014, 2871, 3482, 3695, 3696
AcoI YGGCCR 3 cut(s) 53, 2787, 3078
AcsI RAATTY 9 cut(s) 71, 311, 500, 579, 709, 2503, 2751, 3020, 3743
AcuI CTGAAG 7 cut(s) 261, 914, 1608, 3066, 3081, 4542, 4590
AcvI CACGTG 1 cut(s) 3225
AcyI GRCGYC 1 cut(s) 1304
AdeI CACNNNGTG 1 cut(s) 757
AfaI GTAC 9 cut(s) 151, 646, 676, 888, 1667, 3096, 3880, 4423, 4603
AfiI CCNNNNNNNGG 8 cut(s) 556, 689, 720, 3873, 4061, 4089, 4238, 4322
AflII CTTAAG 2 cut(s) 185, 350
AflIII ACRYGT 3 cut(s) 1331, 2068, 2101
AhlI ACTAGT 1 cut(s) 1793
AjuI GAANNNNNNNTTGG 2 cut(s) 502, 534
AleI CACNNNNGTG 2 cut(s) 874, 1702
Alw21I GWGCWC 2 cut(s) 949, 3121
Alw26I GTCTC 7 cut(s) 998, 1295, 1340, 1855, 3193, 3717, 4285
Alw44I GTGCAC 1 cut(s) 3117
AlwI GGATC 7 cut(s) 818, 1776, 2014, 2871, 3482, 3695, 3696
AlwNI CAGNNNCTG 2 cut(s) 4031, 4455
Aor13HI TCCGGA 1 cut(s) 4007
ApaI GGGCCC 1 cut(s) 1937
ApaLI GTGCAC 1 cut(s) 3117
ApoI RAATTY 9 cut(s) 71, 311, 500, 579, 709, 2503, 2751, 3020, 3743
ArsI GACNNNNNNTTYG 2 cut(s) 296, 328
AseI ATTAAT 1 cut(s) 2963
Asp700I GAANNNNTTC 2 cut(s) 579, 1500
AspLEI GCGC 2 cut(s) 2922, 4245
AsuNHI GCTAGC 1 cut(s) 2711
AvaII GGWCC 6 cut(s) 112, 894, 1859, 2561, 4010, 4313
AxyI CCTNAGG 2 cut(s) 1353, 3915
BaeGI GKGCMC 4 cut(s) 1937, 3121, 3758, 4044
BaeI ACNNNNGTAYC 2 cut(s) 878, 911
BalI TGGCCA 3 cut(s) 55, 2789, 3080
BanI GGYRCC 2 cut(s) 373, 4039
BanII GRGCYC 2 cut(s) 1937, 2559
BbrPI CACGTG 1 cut(s) 3225
BbsI GAAGAC 3 cut(s) 1635, 3162, 3348
Bbv12I GWGCWC 2 cut(s) 949, 3121
BceAI ACGGC 1 cut(s) 3468
BcgI CGANNNNNNTGC 2 cut(s) 1886, 1920
BciVI GTATCC 1 cut(s) 3921
BclI TGATCA 3 cut(s) 847, 2089, 2284
BcoDI GTCTC 7 cut(s) 998, 1295, 1340, 1855, 3193, 3717, 4285
BcuI ACTAGT 1 cut(s) 1793
BfmI CTRYAG 7 cut(s) 171, 398, 440, 1062, 1186, 2847, 3356
BfrI CTTAAG 2 cut(s) 185, 350
BfuAI ACCTGC 1 cut(s) 2302
BfuI GTATCC 1 cut(s) 3921
BglII AGATCT 2 cut(s) 2600, 4145
BmcAI AGTACT 1 cut(s) 1667
Bme18I GGWCC 6 cut(s) 112, 894, 1859, 2561, 4010, 4313
BmiI GGNNCC 9 cut(s) 113, 252, 375, 1372, 1860, 1934, 1935, 4041, 4522
BmrI ACTGGG 1 cut(s) 4071
BmsI GCATC 8 cut(s) 872, 1282, 1549, 1726, 2821, 3328, 4088, 4525
BmtI GCTAGC 1 cut(s) 2715
BmuI ACTGGG 1 cut(s) 4071
BpiI GAAGAC 3 cut(s) 1635, 3162, 3348
BplI GAGNNNNNCTC 2 cut(s) 88, 120
BpmI CTGGAG 1 cut(s) 1307
Bpu10I CCTNAGC 2 cut(s) 105, 4504
BpuEI CTTGAG 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 3225
BsaBI GATNNNNATC 2 cut(s) 3486, 4209
BsaHI GRCGYC 1 cut(s) 1304
BsaI GGTCTC 1 cut(s) 998
BsaWI WCCGGW 2 cut(s) 1624, 4007
Bsc4I CCNNNNNNNGG 8 cut(s) 556, 689, 720, 3873, 4061, 4089, 4238, 4322
Bse1I ACTGG 6 cut(s) 157, 607, 1814, 3142, 4066, 4126
Bse21I CCTNAGG 2 cut(s) 1353, 3915
Bse3DI GCAATG 8 cut(s) 234, 1344, 2364, 2457, 2824, 3471, 3732, 4351
Bse8I GATNNNNATC 2 cut(s) 3486, 4209
BseAI TCCGGA 1 cut(s) 4007
BseJI GATNNNNATC 2 cut(s) 3486, 4209
BseLI CCNNNNNNNGG 8 cut(s) 556, 689, 720, 3873, 4061, 4089, 4238, 4322
BseMI GCAATG 8 cut(s) 234, 1344, 2364, 2457, 2824, 3471, 3732, 4351
BseMII CTCAG 6 cut(s) 107, 119, 1367, 3186, 4518, 4634
BseNI ACTGG 6 cut(s) 157, 607, 1814, 3142, 4066, 4126
BseRI GAGGAG 9 cut(s) 149, 152, 191, 260, 815, 1607, 2198, 4272, 4458
BseSI GKGCMC 4 cut(s) 1937, 3121, 3758, 4044
BseYI CCCAGC 3 cut(s) 4416, 4455, 4479
BsgI GTGCAG 4 cut(s) 779, 1680, 1890, 2280
Bsh1236I CGCG 3 cut(s) 2922, 4165, 4245
Bsh1285I CGRYCG 2 cut(s) 2591, 4249
BshNI GGYRCC 2 cut(s) 373, 4039
BsiEI CGRYCG 2 cut(s) 2591, 4249
BsiHKAI GWGCWC 2 cut(s) 949, 3121
BsiSI CCGG 2 cut(s) 1625, 4008
BslFI GGGAC 6 cut(s) 125, 554, 737, 1156, 1872, 3865
BslI CCNNNNNNNGG 8 cut(s) 556, 689, 720, 3873, 4061, 4089, 4238, 4322
BsmAI GTCTC 7 cut(s) 998, 1295, 1340, 1855, 3193, 3717, 4285
BsmBI CGTCTC 1 cut(s) 1295
BsmFI GGGAC 6 cut(s) 125, 554, 737, 1156, 1872, 3865
Bso31I GGTCTC 1 cut(s) 998
Bsp120I GGGCCC 1 cut(s) 1933
Bsp1286I GDGCHC 6 cut(s) 949, 1937, 2559, 3121, 3758, 4044
Bsp13I TCCGGA 1 cut(s) 4007
Bsp68I TCGCGA 1 cut(s) 4165
BspACI CCGC 9 cut(s) 79, 263, 970, 1717, 4083, 4245, 4470, 4524, 4635
BspCNI CTCAG 6 cut(s) 106, 118, 1366, 3187, 4517, 4633
BspEI TCCGGA 1 cut(s) 4007
BspFNI CGCG 3 cut(s) 2922, 4165, 4245
BspHI TCATGA 1 cut(s) 2082
BspLI GGNNCC 9 cut(s) 113, 252, 375, 1372, 1860, 1934, 1935, 4041, 4522
BspMAI CTGCAG 5 cut(s) 175, 402, 444, 1066, 1190
BspMI ACCTGC 1 cut(s) 2302
BspOI GCTAGC 1 cut(s) 2715
BspPI GGATC 7 cut(s) 818, 1776, 2014, 2871, 3482, 3695, 3696
BspT107I GGYRCC 2 cut(s) 373, 4039
BspTI CTTAAG 2 cut(s) 185, 350
BspTNI GGTCTC 1 cut(s) 998
BsrDI GCAATG 8 cut(s) 234, 1344, 2364, 2457, 2824, 3471, 3732, 4351
BsrI ACTGG 6 cut(s) 157, 607, 1814, 3142, 4066, 4126
BssNI GRCGYC 1 cut(s) 1304
BssT1I CCWWGG 2 cut(s) 115, 4335
Bst4CI ACNGT 5 cut(s) 679, 1242, 1704, 3085, 3780
Bst6I CTCTTC 5 cut(s) 47, 237, 929, 1584, 2380
BstACI GRCGYC 1 cut(s) 1304
BstAFI CTTAAG 2 cut(s) 185, 350
BstAPI GCANNNNNTGC 4 cut(s) 757, 2363, 3116, 4535
BstBAI YACGTR 1 cut(s) 3225
BstDSI CCRYGG 2 cut(s) 3416, 3520
BstFNI CGCG 3 cut(s) 2922, 4165, 4245
BstHHI GCGC 2 cut(s) 2922, 4245
BstMAI GTCTC 7 cut(s) 998, 1295, 1340, 1855, 3193, 3717, 4285
BstMCI CGRYCG 2 cut(s) 2591, 4249
BstNSI RCATGY 4 cut(s) 104, 1335, 2105, 2858
BstSFI CTRYAG 7 cut(s) 171, 398, 440, 1062, 1186, 2847, 3356
BstSLI GKGCMC 4 cut(s) 1937, 3121, 3758, 4044
BstUI CGCG 3 cut(s) 2922, 4165, 4245
BstV2I GAAGAC 3 cut(s) 1635, 3162, 3348
BstX2I RGATCY 6 cut(s) 823, 1781, 2006, 2600, 3487, 4145
BstXI CCANNNNNNTGG 3 cut(s) 1381, 1918, 4416
BstYI RGATCY 6 cut(s) 823, 1781, 2006, 2600, 3487, 4145
Bsu36I CCTNAGG 2 cut(s) 1353, 3915
BsuI GTATCC 1 cut(s) 3921
BtgI CCRYGG 2 cut(s) 3416, 3520
BtgZI GCGATG 1 cut(s) 800
BtsIMutI CAGTG 1 cut(s) 600
BtuMI TCGCGA 1 cut(s) 4165
BveI ACCTGC 1 cut(s) 2302
CaiI CAGNNNCTG 2 cut(s) 4031, 4455
CciI TCATGA 1 cut(s) 2082
CfoI GCGC 2 cut(s) 2922, 4245
CseI GACGC 2 cut(s) 999, 1312
Csp6I GTAC 9 cut(s) 150, 645, 675, 887, 1666, 3095, 3879, 4422, 4602
CspCI CAANNNNNGTGG 2 cut(s) 4093, 4128
CviQI GTAC 9 cut(s) 150, 645, 675, 887, 1666, 3095, 3879, 4422, 4602
DraI TTTAAA 1 cut(s) 1020
DraIII CACNNNGTG 1 cut(s) 757
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
EaeI YGGCCR 3 cut(s) 53, 2787, 3078
Eam1104I CTCTTC 5 cut(s) 47, 237, 929, 1584, 2380
EarI CTCTTC 5 cut(s) 47, 237, 929, 1584, 2380
EciI GGCGGA 1 cut(s) 4485
Eco130I CCWWGG 2 cut(s) 115, 4335
Eco147I AGGCCT 3 cut(s) 702, 1159, 2493
Eco24I GRGCYC 2 cut(s) 1937, 2559
Eco31I GGTCTC 1 cut(s) 998
Eco32I GATATC 2 cut(s) 3613, 3834
Eco47I GGWCC 6 cut(s) 112, 894, 1859, 2561, 4010, 4313
Eco57I CTGAAG 7 cut(s) 261, 914, 1608, 3066, 3081, 4542, 4590
Eco72I CACGTG 1 cut(s) 3225
Eco81I CCTNAGG 2 cut(s) 1353, 3915
EcoO109I RGGNCCY 4 cut(s) 112, 218, 1933, 1934
EcoRI GAATTC 4 cut(s) 579, 709, 2751, 3020
EcoRV GATATC 2 cut(s) 3613, 3834
EcoT14I CCWWGG 2 cut(s) 115, 4335
EcoT38I GRGCYC 2 cut(s) 1937, 2559
ErhI CCWWGG 2 cut(s) 115, 4335
Esp3I CGTCTC 1 cut(s) 1295
FalI AAGNNNNNCTT 6 cut(s) 1091, 1123, 1184, 1216, 3039, 3071
FaqI GGGAC 6 cut(s) 125, 554, 737, 1156, 1872, 3865
FauNDI CATATG 2 cut(s) 1380, 4234
FbaI TGATCA 3 cut(s) 847, 2089, 2284
FblI GTMKAC 2 cut(s) 303, 3355
FriOI GRGCYC 2 cut(s) 1937, 2559
GlaI GCGC 2 cut(s) 2921, 4244
GsaI CCCAGC 3 cut(s) 4420, 4459, 4483
GsuI CTGGAG 1 cut(s) 1307
HapII CCGG 2 cut(s) 1625, 4008
HgaI GACGC 2 cut(s) 999, 1312
HhaI GCGC 2 cut(s) 2922, 4245
Hin1I GRCGYC 1 cut(s) 1304
Hin6I GCGC 2 cut(s) 2920, 4243
HinP1I GCGC 2 cut(s) 2920, 4243
HincII GTYRAC 3 cut(s) 304, 1791, 2035
HindII GTYRAC 3 cut(s) 304, 1791, 2035
HindIII AAGCTT 5 cut(s) 182, 347, 1497, 1598, 4401
HpaI GTTAAC 1 cut(s) 2035
HpaII CCGG 2 cut(s) 1625, 4008
Hpy166II GTNNAC 8 cut(s) 304, 1093, 1461, 1791, 2035, 3119, 3356, 3556
Hpy8I GTNNAC 8 cut(s) 304, 1093, 1461, 1791, 2035, 3119, 3356, 3556
Hpy99I CGWCG 3 cut(s) 308, 993, 3753
HpyCH4III ACNGT 5 cut(s) 679, 1242, 1704, 3085, 3780
HpyCH4IV ACGT 4 cut(s) 2031, 2068, 3224, 3582
HpySE526I ACGT 4 cut(s) 2031, 2068, 3224, 3582
Hsp92I GRCGYC 1 cut(s) 1304
HspAI GCGC 2 cut(s) 2920, 4243
Kpn2I TCCGGA 1 cut(s) 4007
Ksp22I TGATCA 3 cut(s) 847, 2089, 2284
KspAI GTTAAC 1 cut(s) 2035
LmnI GCTCC 5 cut(s) 1370, 1431, 2027, 2117, 4259
LweI GCATC 8 cut(s) 872, 1282, 1549, 1726, 2821, 3328, 4088, 4525
MaeII ACGT 4 cut(s) 2031, 2068, 3224, 3582
MaeIII GTNAC 8 cut(s) 1050, 1226, 1257, 1609, 2336, 2476, 2945, 3212
MfeI CAATTG 3 cut(s) 510, 1253, 1491
MflI RGATCY 6 cut(s) 823, 1781, 2006, 2600, 3487, 4145
MhlI GDGCHC 6 cut(s) 949, 1937, 2559, 3121, 3758, 4044
MlsI TGGCCA 3 cut(s) 55, 2789, 3080
MluNI TGGCCA 3 cut(s) 55, 2789, 3080
MlyI GAGTC 3 cut(s) 3634, 4013, 4568
MmeI TCCRAC 4 cut(s) 2182, 2718, 3772, 4097
Mox20I TGGCCA 3 cut(s) 55, 2789, 3080
MroI TCCGGA 1 cut(s) 4007
MroXI GAANNNNTTC 2 cut(s) 579, 1500
MscI TGGCCA 3 cut(s) 55, 2789, 3080
MslI CAYNNNNRTG 5 cut(s) 839, 874, 1702, 3897, 4215
Msp20I TGGCCA 3 cut(s) 55, 2789, 3080
MspA1I CMGCKG 4 cut(s) 445, 1717, 4455, 4526
MspCI CTTAAG 2 cut(s) 185, 350
MspI CCGG 2 cut(s) 1625, 4008
MunI CAATTG 3 cut(s) 510, 1253, 1491
MvnI CGCG 3 cut(s) 2922, 4165, 4245
NdeI CATATG 2 cut(s) 1380, 4234
NheI GCTAGC 1 cut(s) 2711
NlaIV GGNNCC 9 cut(s) 113, 252, 375, 1372, 1860, 1934, 1935, 4041, 4522
NmeAIII GCCGAG 1 cut(s) 4301
NmuCI GTSAC 4 cut(s) 1050, 1257, 2945, 3212
NruI TCGCGA 1 cut(s) 4165
NspI RCATGY 4 cut(s) 104, 1335, 2105, 2858
OliI CACNNNNGTG 2 cut(s) 874, 1702
PaeI GCATGC 1 cut(s) 104
PagI TCATGA 1 cut(s) 2082
PasI CCCWGGG 2 cut(s) 1911, 1937
PceI AGGCCT 3 cut(s) 702, 1159, 2493
PciI ACATGT 2 cut(s) 1331, 2101
PcsI WCGNNNNNNNCGW 1 cut(s) 3745
PdmI GAANNNNTTC 2 cut(s) 579, 1500
Ple19I CGATCG 1 cut(s) 2591
PleI GAGTC 3 cut(s) 3634, 4012, 4568
PmaCI CACGTG 1 cut(s) 3225
PmlI CACGTG 1 cut(s) 3225
PpsI GAGTC 3 cut(s) 3634, 4012, 4568
Ppu21I YACGTR 1 cut(s) 3225
PpuMI RGGWCCY 1 cut(s) 112
PscI ACATGT 2 cut(s) 1331, 2101
PshBI ATTAAT 1 cut(s) 2963
Psp1406I AACGTT 1 cut(s) 2031
Psp5II RGGWCCY 1 cut(s) 112
PspCI CACGTG 1 cut(s) 3225
PspFI CCCAGC 3 cut(s) 4416, 4455, 4479
PspN4I GGNNCC 9 cut(s) 113, 252, 375, 1372, 1860, 1934, 1935, 4041, 4522
PspOMI GGGCCC 1 cut(s) 1933
PspPPI RGGWCCY 1 cut(s) 112
PsrI GAACNNNNNNTAC 2 cut(s) 3248, 3280
PstI CTGCAG 5 cut(s) 175, 402, 444, 1066, 1190
PstNI CAGNNNCTG 2 cut(s) 4031, 4455
PsuI RGATCY 6 cut(s) 823, 1781, 2006, 2600, 3487, 4145
PvuI CGATCG 1 cut(s) 2591
PvuII CAGCTG 2 cut(s) 445, 4455
RruI TCGCGA 1 cut(s) 4165
RsaI GTAC 9 cut(s) 151, 646, 676, 888, 1667, 3096, 3880, 4423, 4603
RsaNI GTAC 9 cut(s) 150, 645, 675, 887, 1666, 3095, 3879, 4422, 4602
RseI CAYNNNNRTG 5 cut(s) 839, 874, 1702, 3897, 4215
SalI GTCGAC 1 cut(s) 302
ScaI AGTACT 1 cut(s) 1667
SchI GAGTC 3 cut(s) 3634, 4013, 4568
SduI GDGCHC 6 cut(s) 949, 1937, 2559, 3121, 3758, 4044
SfaNI GCATC 8 cut(s) 872, 1282, 1549, 1726, 2821, 3328, 4088, 4525
SfcI CTRYAG 7 cut(s) 171, 398, 440, 1062, 1186, 2847, 3356
SinI GGWCC 6 cut(s) 112, 894, 1859, 2561, 4010, 4313
SmiMI CAYNNNNRTG 5 cut(s) 839, 874, 1702, 3897, 4215
SmlI CTYRAG 3 cut(s) 185, 227, 350
SmoI CTYRAG 3 cut(s) 185, 227, 350
SpeI ACTAGT 1 cut(s) 1793
SphI GCATGC 1 cut(s) 104
SseBI AGGCCT 3 cut(s) 702, 1159, 2493
SsiI CCGC 9 cut(s) 79, 263, 970, 1717, 4083, 4245, 4470, 4524, 4635
SspI AATATT 3 cut(s) 2613, 3313, 3448
StuI AGGCCT 3 cut(s) 702, 1159, 2493
StyI CCWWGG 2 cut(s) 115, 4335
TaaI ACNGT 5 cut(s) 679, 1242, 1704, 3085, 3780
TaiI ACGT 4 cut(s) 2034, 2071, 3227, 3585
TaqI TCGA 8 cut(s) 303, 988, 2496, 3301, 3324, 4201, 4255, 4577
TatI WGTACW 2 cut(s) 1665, 3878
TauI GCSGC 2 cut(s) 82, 4526
TscAI CASTG 1 cut(s) 607
TseFI GTSAC 4 cut(s) 1050, 1257, 2945, 3212
Tsp45I GTSAC 4 cut(s) 1050, 1257, 2945, 3212
TspGWI ACGGA 9 cut(s) 288, 1494, 1842, 1885, 2062, 2340, 3433, 3617, 3889
TspRI CASTG 1 cut(s) 607
Vha464I CTTAAG 2 cut(s) 185, 350
VneI GTGCAC 1 cut(s) 3117
VpaK11BI GGWCC 6 cut(s) 112, 894, 1859, 2561, 4010, 4313
VspI ATTAAT 1 cut(s) 2963
XapI RAATTY 9 cut(s) 71, 311, 500, 579, 709, 2503, 2751, 3020, 3743
XceI RCATGY 4 cut(s) 104, 1335, 2105, 2858
XcmI CCANNNNNNNNNTGG 1 cut(s) 3899
XmiI GTMKAC 2 cut(s) 303, 3355
XmnI GAANNNNTTC 2 cut(s) 579, 1500
ZrmI AGTACT 1 cut(s) 1667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.