MD10G1173400.v1.1

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009494
Physical Location & Seq
Reverse (-)
26583571 .. 26590774
7204 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1173400.v1.1.491

Sequence Viewer

Length: 4680 bp
ATGGGCAAAGGTGTTGTCTCCGACGAGGAAGATGAGATGATTCATGGAGAGGACGAGGACAGGTCGCTTGTTGACGACGTTGCAGTGGATGACTCTGACGGTGGCGGTGACGGTGACGACGTGGAAGATGAAGAGGAGGAGGGCGGTCTGGATGAATATGAGAAGGATGATTTTCTAGTGGATGATGTCGAGGATGAAGAGGAGGAGGAAGAAGGGAGAGCGGACAGTGATGAAGAAGAAAGACAGAAGAAGAAGAAAAAAAAGAAAAGGGAGGAGTATGTCCTTGATGACGATGACTATGATCTCCTGGAGGACAATAATGTGTATGCCTTCCGCAGAAAGGGGAAATTTAAGCGACTGAAAAAAGCTCAGAGGAACTCTGAGGGGGTATCTGATGGGAAGACGATTGAGCAATCTTTATTTGATGGTGAAGAAGGTTATCCGCTTGAGGACATTGCAGAAGAGGAGGAACCAGAAGCAGAAGCCGATGGAGATGTTGGTGAAGATGATATGGCAGACTTTATTGTCGATGATGAAGAGTTTGATGTGACTGGATCTCCAGTGAGACAAGGGAAGGTTAAGAGAAGAAAATCTACAAAGGCACCAGAAGTCCCATCATCTTCTTTGCAGGAAGCACATGATATATTTGGTGATCCTCAACAACTGATAGAACTTCGAAAGCAAGGTTTGGATTCCGGGGAATGGAGGAATATGAGGATTAAAGATCAGTTTGAACCCAGTGTTCTTTCTGAAAAGTATATGACAGACAAGGATGAACAGATTCAGGCGCTTGATATTCCGGAAAGAATTCAGATATATGAGACATGCACTGGTTCTCCTCCACTGAATGCAAAACGTATAGATGATGAGAGTACCTGGATATATGATCAACTTACGAGTGGTATAAGACCCTTCTATGGCATGACAGACTTGGGGAAATCTATAAGCAGGGATGACATCAGAAATTTTTTGGAGTTGCACCATGTGCAAATGTTCGATATTCCCTTTATTGCTATGTATCGGAAAGAGGAGTGTCTAAGCTTATTGAAAGATCCCAAACACCTTGAATTGGAAGACGAAAACCAGGACAAAAATAAGAACAAACCTGAGCTAAAGTGGCACAGATTACTTTGGACGATTCAGGACTTGGACAGAAAATGGCTGCTGCTTCAGAAGCGAAAAAGTGCATTGAAATCATACTACAAGAAGCGGTTTGAGGTAGAATCTCAATGTATATCTGAGGAGACCAGACTCACATTGAATCAGCAGCTTTTTGAATCAGTTATGAAGTCACTTGAGGCTGCAAAATCAGAGAGAGAAGTTGATGATGCTGATGCCAAGTTTAACTTGCATTTTCCTCCAGGCGAGGGAGGTGTGGATGCAGGACAATATAAGAGGCCGAAGAGGAAATCACTTTATAGTATCTGCATCAAGGCTGGCCTGTGGCATGCTGCAAGAAAGTTCGGCTACAGCTCTGAGCAATTTGGACTGCAATTATCACTAGAAAATATGAGAATGGATGAGTTGGAGGATGCTAAGGAGACACCAGAGGAGATGGCTTCCACCTTTACATGTGCAATGTTCGATACACCTCAAGCCGTGCTCAAAGGAGCTAGGCATATGGCAGCTGTTGAGATAAGTTGGGAGCCATGTGTCAGGAAGTATGTTCGAAGCAATTACTTGGATATAACTGAGATATCAACAAACCCAACACTTGAAGGAAATGTTGCAATAGATGCTTCTCATCAGTTTGCAGGGGTGAAGTGGCTGAAAAGAAAGCCATTAAATAGATTCGAGGGCGCGCAATGGCTGCTTATCCAGAAGGCTGAAGAGGAGAAGCTTCTTCAAGTTACTATTCAGCTGCCGGAAGACAGACTAAATAAGTTGATCAGCGACTTTAATGAGTACTATCTTAGTGATGGTGTTAGTAAATCTGCACAACTGTGGAATGAGCAGCGGAAGCTGATATTGCAGGATGCACTTTTTAATTTCCTTCTACCTTCAATTGAGAAAGAAGCAAGATCCTTATTGACTAGTAGAGCAAAACACTGGTTACGTGTGGAATACGGAAATGCTTTGTGGAATCAAGTCTCTGTGGGACCTTATCAACGGAAGGAAAATGATGGTAGCTCAAACAATGAAGCTGCACCGAAGGTCATGGCTTGCTTTTGGGGACCTGGTAACCCAGCAACCACTTTTGTGATGTTAGATTCATCTGGGGAGGTGCTAGATGTACTTTATGCCGGGGCCCTCTCTTTACGCTTCCTCAATGCTAATGACGAGAAGCGTAAAAAGGATGAACAAGAACGCATTTTGAAGTTCATGATTGATCATCAACCACACGTCGTCGTCCTAGGGGGTGTTAACTTGTCTTGTACTCGCCTGAAGGACGATATCTATGAGATAATTTATAAGATGGTGGAGGAGCATCCAAGAGATGTTGGGGCTGATATGGATGGCCTAAGCATTGTACATGGGGATGAATCTTTGGCTCGCCTATATGAAAATTCTCGGATTTCATCTGATCAGCTACCAATGCAGCTGCAGTCAGGCATTGTCAAGAAGGCTGTGGCTCTTGGGCGTTATCTTCAAAATCCATTGGCCATGGTTGCCACGCTTTGTGGACCAGGACGAGAGATCTTATCTTGGAAAATCTGCGCTCTTGAGCACTTTCTCACCCCAGATGAGAAGTATGAGGTGGTTGAACAGGTCATGGTAGATGTTACAAACCAGGTAGGCATTGACCTAAATTTGGCTATAAGCCACGAATGGTTATTTGCACCACTTCAATTCATTTCTGGACTCGGACCTAGAAAGGCAGCCTCGTTGCAGAAGATGTTGGTAGGCTTTGGTGAAGCAGTATATACACGAAAGGACATGTCAAAGGACACGTCAAATCCACCAACACTAAAGGACAAGTTAAAGAGAAAATACGGGCTTGATAATAGGGTTTTTATTAATGCTTGTGGTTTCCTGCGTGTTCGGCTGAGTGGGTCGGCTGGTAGCAGCTACACCGATATTGATCTGCTGGATGATACCAGAATCAATCCGGAATCTTATCAACTTGCAGAACAATTGGCGAAAGATGTTTATGATGTGGAAGGTCGCAATGATGATGATGATGTGGTGGGGTTCGTCAGGGAGCATCCTAAATATCTAAAGAACATCGATGTTGAAGAATATGCTAAAAGCAAACGGTGTGAGAAAAAGATAATTACATTGTATGATATAAGGAGAGAATTAATACAGGGTTTTCAGGACTGGCGTAAAAAATATGAAGAACCAACCCAGGATGAAGAATTCTACATGATTACAGGTGAAACGGAGGATACCCTTGCCGAAGGAAAAATTGTGCAGGTCACAGTTCGCAGGGTGCAAGCTGATCGTGCACAACTTGCTCTTGAATGTGGATTGACTGGTATTCTTATGAAAGAAGACTATCATCCAGATGATTGGAGGGGTATTTCTGACTTGTCTGATAGACTGAATGAGGGTGATATTCTCACTTGCAAGATCAAATCAATTCAAAAGAAAAGTTACCGGGTGTTCCTAGCTTATAGAGAGAGTGAGATTAGAAATGACCGATCTCACATTCAGAATCTTGATACCTATTACCAAGAAGACTGGAAAAGTCTACAGAGTGAGCAAGAAAAAGCTCAGAAGAGTATGCAGCTGGAAAAGAAGAATTTCAATCCAAGGATGATCGTTCATCCTCGCTTCCAGAATATATCTGCAGATGAATCGATGAGGTTATTGTCTGATAAGGATCCTGGGGAAAGCATTTTTCGTCCTAGTTCCCGTGGGCCTTCTCATTTATCCTTAACCCTCAAGATTTATGATGGAGTTTATGCTCATAAAGACATAGTTGAAGGTGGAAAGGAAAAGAAGGACATTACGAGCATACTCCGTATTGGAAAGACCCTGACCATTGGGGAGGACACTTTTGAGGATTTGGACGAGGTGGTGGATCGTTATGTTGATCCATTAGTGGCTCAGTTGAAGGCAATGCTAAACTATCGAAAGTTCAGGAGGGGAACAAAGGCAGAGGTTGATGCTCTTTTGAAGATTGAGAAATTGGAGAATCCCGCGCGGATAGTTTATAGTTTTGGGGTATCACATGAATATCCAGGCACGATAATTCTGACCTATATTCGGAGTTCAAATCCACACCACGAGTTAGTTGGAATGTATCCAAAGGGATTCAAGTTCAGAAAACGGATGTTTAAGAAAATTGATCAGCTTGTAGAGTATTTTCAACGCCATGTCAATGACCCTCCGCAAGAGTTAGGGCCACCAATTAGATCAGTTGCTGCTATTGTGCCAATGCGAAGCCCTGCAACTCGGGGTTCTTCAGGGGCATCCGTGGGCAGTGGTTGGGGTGGTTCAACCAATGATGGTGGGCGGAGAAGCCAGACTTCTGGCAGGGATCGATCATCTACATCGAGTTCACGAACAGGCCGAAATGATATGGGCAACTTTCCGGGTGCAAAGGTTCAAAACTCACCGGGAAGGGAAGCTTTCCCAGGTGGCTGGGGTGCCGGTGGAAGTGGCAGTGGCAGTGGCTGGGGTGGCAAGAGCAATGATGGATGGGGTCAAGGAAGTGGTGGTGCATATGGAGATGTTGTTGATCCTAGGGCTTCAGGCTGGGATTCTGACCCAAAACGAAGAAGGTTCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

1560

Amino Acids

177.48

Weight (kDa)

5.13

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT6_acidic PF14632 39 - 125 6.3e-13 Acidic N-terminal SPT6
HTH_44 PF14641 282 - 403 7.9e-07 Helix-turn-helix DNA-binding domain of SPT6
YqgF PF14639 717 - 874 5.3e-15 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 878 - 993 9.3e-14 Helix-hairpin-helix motif
Spt6_S1 PF21710 1101 - 1190 1.5e-09 Spt6, S1/OB domain
SH2_2 PF14633 1208 - 1421 4.6e-65 SH2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2412
AasI GACNNNNNNGTC 1 cut(s) 524
Acc36I ACCTGC 1 cut(s) 3346
AccB1I GGYRCC 2 cut(s) 601, 4535
AccBSI CCGCTC 1 cut(s) 221
AccI GTMKAC 1 cut(s) 3634
AccII CGCG 3 cut(s) 1802, 4088, 4090
AccIII TCCGGA 2 cut(s) 799, 3049
AcoI YGGCCR 1 cut(s) 2601
AcsI RAATTY 7 cut(s) 347, 807, 964, 2506, 2749, 3299, 3685
AcuI CTGAAG 5 cut(s) 1154, 1848, 2405, 4335, 4623
AdeI CACNNNGTG 1 cut(s) 985
AfaI GTAC 5 cut(s) 874, 1907, 2235, 2377, 2472
AfiI CCNNNNNNNGG 7 cut(s) 340, 702, 917, 1069, 1367, 2752, 4152
AflIII ACRYGT 5 cut(s) 1571, 2056, 2341, 2877, 2889
AhlI ACTAGT 1 cut(s) 2033
AjiI CACGTC 3 cut(s) 121, 2344, 2892
AleI CACNNNNGTG 2 cut(s) 1942, 2198
Alw21I GWGCWC 3 cut(s) 1605, 2670, 3391
Alw26I GTCTC 6 cut(s) 22, 559, 815, 1238, 1535, 2095
Alw44I GTGCAC 1 cut(s) 3387
AlwNI CAGNNNCTG 2 cut(s) 4310, 4563
Ama87I CYCGRG 1 cut(s) 4341
Aor13HI TCCGGA 2 cut(s) 799, 3049
AoxI GGCC 8 cut(s) 1397, 1438, 2247, 2458, 2601, 3803, 4289, 4456
ApaI GGGCCC 1 cut(s) 2251
ApaLI GTGCAC 1 cut(s) 3387
ApoI RAATTY 7 cut(s) 347, 807, 964, 2506, 2749, 3299, 3685
ArsI GACNNNNNNTTYG 3 cut(s) 32, 2876, 2908
AseI ATTAAT 2 cut(s) 2958, 3242
Asp700I GAANNNNTTC 3 cut(s) 780, 807, 3686
AspA2I CCTAGG 2 cut(s) 2353, 4631
AspLEI GCGC 5 cut(s) 790, 1802, 1804, 2660, 4090
AspS9I GGNCC 8 cut(s) 2099, 2174, 2247, 2248, 2624, 2807, 3803, 4289
AsuC2I CCSGG 5 cut(s) 697, 2245, 3542, 4482, 4506
AsuII TTCGAA 2 cut(s) 676, 1669
AvaI CYCGRG 1 cut(s) 4341
AvaII GGWCC 4 cut(s) 2099, 2174, 2624, 2807
AvrII CCTAGG 2 cut(s) 2353, 4631
BaeGI GKGCMC 2 cut(s) 2251, 3391
BalI TGGCCA 1 cut(s) 2603
BamHI GGATCC 1 cut(s) 3766
BanI GGYRCC 2 cut(s) 601, 4535
BanII GRGCYC 1 cut(s) 2251
BarI GAAGNNNNNNTAC 2 cut(s) 577, 609
BauI CACGAG 1 cut(s) 4172
BbsI GAAGAC 5 cut(s) 407, 1080, 1875, 3441, 3627
Bbv12I GWGCWC 3 cut(s) 1605, 2670, 3391
BceAI ACGGC 1 cut(s) 1583
BcgI CGANNNNNNTGC 4 cut(s) 3365, 3399, 3723, 3757
BciVI GTATCC 2 cut(s) 3322, 4200
BclI TGATCA 5 cut(s) 886, 1887, 2329, 2524, 4234
BcnI CCSGG 5 cut(s) 697, 2245, 3542, 4482, 4506
BcoDI GTCTC 6 cut(s) 22, 559, 815, 1238, 1535, 2095
BcuI ACTAGT 1 cut(s) 2033
BfmI CTRYAG 4 cut(s) 1468, 2543, 3635, 3732
BfoI RGCGCY 1 cut(s) 791
BfuAI ACCTGC 1 cut(s) 3346
BfuI GTATCC 2 cut(s) 3322, 4200
BglII AGATCT 1 cut(s) 2637
BlnI CCTAGG 2 cut(s) 2353, 4631
BmcAI AGTACT 1 cut(s) 1907
Bme18I GGWCC 4 cut(s) 2099, 2174, 2624, 2807
BmeT110I CYCGRG 1 cut(s) 4341
BmgBI CACGTC 3 cut(s) 121, 2344, 2892
BmgT120I GGNCC 8 cut(s) 2099, 2174, 2247, 2248, 2624, 2807, 3803, 4289
BmiI GGNNCC 9 cut(s) 471, 603, 1647, 2100, 2175, 2248, 2249, 3768, 4537
BmrI ACTGGG 1 cut(s) 732
BmuI ACTGGG 1 cut(s) 732
BpiI GAAGAC 5 cut(s) 407, 1080, 1875, 3441, 3627
BpmI CTGGAG 3 cut(s) 329, 543, 1344
Bpu10I CCTNAGC 3 cut(s) 1107, 1536, 2462
Bpu14I TTCGAA 2 cut(s) 676, 1669
BpuEI CTTGAG 5 cut(s) 467, 1316, 1578, 2684, 3812
BpuMI CCSGG 5 cut(s) 697, 2245, 3542, 4482, 4506
Bsa29I ATCGAT 3 cut(s) 3168, 3743, 4429
BsaAI YACGTR 1 cut(s) 2057
BsaBI GATNNNNATC 2 cut(s) 3021, 3765
BsaI GGTCTC 1 cut(s) 1238
BsaWI WCCGGW 2 cut(s) 799, 3049
BsaXI ACNNNNNCTCC 6 cut(s) 541, 571, 820, 850, 4608, 4638
Bsc4I CCNNNNNNNGG 7 cut(s) 340, 702, 917, 1069, 1367, 2752, 4152
Bse118I RCCGGY 1 cut(s) 4538
Bse1I ACTGG 8 cut(s) 556, 560, 738, 835, 2054, 3266, 3421, 3629
Bse3DI GCAATG 6 cut(s) 453, 1584, 1811, 3115, 4011, 4585
Bse8I GATNNNNATC 2 cut(s) 3021, 3765
BseAI TCCGGA 2 cut(s) 799, 3049
BseCI ATCGAT 3 cut(s) 3168, 3743, 4429
BseJI GATNNNNATC 2 cut(s) 3021, 3765
BseLI CCNNNNNNNGG 7 cut(s) 340, 702, 917, 1069, 1367, 2752, 4152
BseMI GCAATG 6 cut(s) 453, 1584, 1811, 3115, 4011, 4585
BseMII CTCAG 9 cut(s) 372, 383, 1098, 1230, 1467, 1683, 2978, 3671, 4007
BseNI ACTGG 8 cut(s) 556, 560, 738, 835, 2054, 3266, 3421, 3629
BsePI GCGCGC 1 cut(s) 1800
BseSI GKGCMC 2 cut(s) 2251, 3391
BseYI CCCAGC 4 cut(s) 2185, 4530, 4563, 4644
BsgI GTGCAG 3 cut(s) 1920, 2130, 3374
Bsh1236I CGCG 3 cut(s) 1802, 4088, 4090
BshFI GGCC 8 cut(s) 1399, 1440, 2249, 2460, 2603, 3805, 4291, 4458
BshNI GGYRCC 2 cut(s) 601, 4535
BshVI ATCGAT 3 cut(s) 3168, 3743, 4429
BsiHKAI GWGCWC 3 cut(s) 1605, 2670, 3391
BsiHKCI CYCGRG 1 cut(s) 4341
BsiSI CCGG 9 cut(s) 696, 800, 1865, 2244, 3050, 3541, 4481, 4505, 4539
BslFI GGGAC 3 cut(s) 596, 2112, 2187
BslI CCNNNNNNNGG 7 cut(s) 340, 702, 917, 1069, 1367, 2752, 4152
BsmAI GTCTC 6 cut(s) 22, 559, 815, 1238, 1535, 2095
BsmFI GGGAC 3 cut(s) 596, 2112, 2187
BsmI GAATGC 1 cut(s) 853
BsnI GGCC 8 cut(s) 1399, 1440, 2249, 2460, 2603, 3805, 4291, 4458
Bso31I GGTCTC 1 cut(s) 1238
BsoBI CYCGRG 1 cut(s) 4341
Bsp119I TTCGAA 2 cut(s) 676, 1669
Bsp120I GGGCCC 1 cut(s) 2247
Bsp1286I GDGCHC 4 cut(s) 1605, 2251, 2670, 3391
Bsp13I TCCGGA 2 cut(s) 799, 3049
Bsp1407I TGTACA 1 cut(s) 2470
Bsp19I CCATGG 1 cut(s) 2604
BspANI GGCC 8 cut(s) 1399, 1440, 2249, 2460, 2603, 3805, 4291, 4458
BspCNI CTCAG 9 cut(s) 373, 382, 1099, 1231, 1468, 1684, 2979, 3670, 4006
BspDI ATCGAT 3 cut(s) 3168, 3743, 4429
BspEI TCCGGA 2 cut(s) 799, 3049
BspFNI CGCG 3 cut(s) 1802, 4088, 4090
BspHI TCATGA 1 cut(s) 2322
BspLI GGNNCC 9 cut(s) 471, 603, 1647, 2100, 2175, 2248, 2249, 3768, 4537
BspMAI CTGCAG 2 cut(s) 2547, 3736
BspMI ACCTGC 1 cut(s) 3346
BspT104I TTCGAA 2 cut(s) 676, 1669
BspT107I GGYRCC 2 cut(s) 601, 4535
BspTNI GGTCTC 1 cut(s) 1238
BsrBI CCGCTC 1 cut(s) 221
BsrDI GCAATG 6 cut(s) 453, 1584, 1811, 3115, 4011, 4585
BsrFI RCCGGY 1 cut(s) 4538
BsrGI TGTACA 1 cut(s) 2470
BsrI ACTGG 8 cut(s) 556, 560, 738, 835, 2054, 3266, 3421, 3629
BssAI RCCGGY 1 cut(s) 4538
BssHII GCGCGC 1 cut(s) 1800
BssSI CACGAG 1 cut(s) 4172
BssT1I CCWWGG 4 cut(s) 2353, 2604, 3695, 4631
Bst2BI CACGAG 1 cut(s) 4172
Bst4CI ACNGT 6 cut(s) 101, 113, 227, 1944, 3198, 3364
Bst6I CTCTTC 7 cut(s) 126, 192, 456, 531, 1397, 1824, 3656
BstAPI GCANNNNNTGC 4 cut(s) 985, 1736, 1810, 3395
BstAUI TGTACA 1 cut(s) 2470
BstBAI YACGTR 1 cut(s) 2057
BstBI TTCGAA 2 cut(s) 676, 1669
BstC8I GCNNGC 6 cut(s) 1438, 1449, 1802, 2164, 2494, 3378
BstDSI CCRYGG 3 cut(s) 2604, 3799, 4362
BstEII GGTNACC 1 cut(s) 2180
BstFNI CGCG 3 cut(s) 1802, 4088, 4090
BstH2I RGCGCY 1 cut(s) 791
BstHHI GCGC 5 cut(s) 790, 1802, 1804, 2660, 4090
BstMAI GTCTC 6 cut(s) 22, 559, 815, 1238, 1535, 2095
BstNSI RCATGY 4 cut(s) 828, 1451, 1575, 2881
BstPI GGTNACC 1 cut(s) 2180
BstSFI CTRYAG 4 cut(s) 1468, 2543, 3635, 3732
BstSLI GKGCMC 2 cut(s) 2251, 3391
BstUI CGCG 3 cut(s) 1802, 4088, 4090
BstV2I GAAGAC 5 cut(s) 407, 1080, 1875, 3441, 3627
BstX2I RGATCY 5 cut(s) 554, 1051, 2021, 2637, 3766
BstXI CCANNNNNNTGG 3 cut(s) 3453, 4418, 4530
BstYI RGATCY 5 cut(s) 554, 1051, 2021, 2637, 3766
Bsu15I ATCGAT 3 cut(s) 3168, 3743, 4429
BsuI GTATCC 2 cut(s) 3322, 4200
BsuRI GGCC 8 cut(s) 1399, 1440, 2249, 2460, 2603, 3805, 4291, 4458
BsuTUI ATCGAT 3 cut(s) 3168, 3743, 4429
BtgI CCRYGG 3 cut(s) 2604, 3799, 4362
BtrI CACGTC 3 cut(s) 121, 2344, 2892
BtsI GCAGTG 4 cut(s) 90, 4375, 4558, 4564
BveI ACCTGC 1 cut(s) 3346
Cac8I GCNNGC 6 cut(s) 1438, 1449, 1802, 2164, 2494, 3378
CaiI CAGNNNCTG 2 cut(s) 4310, 4563
CciI TCATGA 1 cut(s) 2322
CfoI GCGC 5 cut(s) 790, 1802, 1804, 2660, 4090
Cfr10I RCCGGY 1 cut(s) 4538
Cfr13I GGNCC 8 cut(s) 2099, 2174, 2247, 2248, 2624, 2807, 3803, 4289
ClaI ATCGAT 3 cut(s) 3168, 3743, 4429
CsiI ACCWGGT 2 cut(s) 2176, 2730
Csp6I GTAC 5 cut(s) 873, 1906, 2234, 2376, 2471
CspCI CAANNNNNGTGG 2 cut(s) 4378, 4413
CviQI GTAC 5 cut(s) 873, 1906, 2234, 2376, 2471
DraIII CACNNNGTG 1 cut(s) 985
DrdI GACNNNNNNGTC 1 cut(s) 524
DseDI GACNNNNNNGTC 1 cut(s) 524
EaeI YGGCCR 1 cut(s) 2601
Eam1104I CTCTTC 7 cut(s) 126, 192, 456, 531, 1397, 1824, 3656
EarI CTCTTC 7 cut(s) 126, 192, 456, 531, 1397, 1824, 3656
EciI GGCGGA 1 cut(s) 4417
Eco130I CCWWGG 4 cut(s) 2353, 2604, 3695, 4631
Eco24I GRGCYC 1 cut(s) 2251
Eco31I GGTCTC 1 cut(s) 1238
Eco32I GATATC 2 cut(s) 1698, 2395
Eco47I GGWCC 4 cut(s) 2099, 2174, 2624, 2807
Eco57I CTGAAG 5 cut(s) 1154, 1848, 2405, 4335, 4623
Eco88I CYCGRG 1 cut(s) 4341
Eco91I GGTNACC 1 cut(s) 2180
EcoO109I RGGNCCY 4 cut(s) 2099, 2174, 2247, 2248
EcoO65I GGTNACC 1 cut(s) 2180
EcoRI GAATTC 2 cut(s) 807, 3299
EcoRV GATATC 2 cut(s) 1698, 2395
EcoT14I CCWWGG 4 cut(s) 2353, 2604, 3695, 4631
EcoT38I GRGCYC 1 cut(s) 2251
ErhI CCWWGG 4 cut(s) 2353, 2604, 3695, 4631
FalI AAGNNNNNCTT 6 cut(s) 1331, 1363, 4399, 4431, 4501, 4533
FaqI GGGAC 3 cut(s) 596, 2112, 2187
FauI CCCGC 1 cut(s) 4093
FauNDI CATATG 2 cut(s) 1620, 4612
FbaI TGATCA 5 cut(s) 886, 1887, 2329, 2524, 4234
FblI GTMKAC 1 cut(s) 3634
FriOI GRGCYC 1 cut(s) 2251
GlaI GCGC 5 cut(s) 789, 1801, 1803, 2659, 4089
GsaI CCCAGC 4 cut(s) 2189, 4534, 4567, 4648
GsuI CTGGAG 3 cut(s) 329, 543, 1344
HaeII RGCGCY 1 cut(s) 791
HaeIII GGCC 8 cut(s) 1399, 1440, 2249, 2460, 2603, 3805, 4291, 4458
HapII CCGG 9 cut(s) 696, 800, 1865, 2244, 3050, 3541, 4481, 4505, 4539
HhaI GCGC 5 cut(s) 790, 1802, 1804, 2660, 4090
Hin6I GCGC 5 cut(s) 788, 1800, 1802, 2658, 4088
HinP1I GCGC 5 cut(s) 788, 1800, 1802, 2658, 4088
HincII GTYRAC 2 cut(s) 73, 2365
HindII GTYRAC 2 cut(s) 73, 2365
HindIII AAGCTT 3 cut(s) 1039, 1838, 4515
HpaI GTTAAC 1 cut(s) 2365
HpaII CCGG 9 cut(s) 696, 800, 1865, 2244, 3050, 3541, 4481, 4505, 4539
Hpy166II GTNNAC 6 cut(s) 73, 2365, 2624, 3389, 3635, 4448
Hpy8I GTNNAC 6 cut(s) 73, 2365, 2624, 3389, 3635, 4448
Hpy99I CGWCG 5 cut(s) 26, 80, 122, 2348, 2351
HpyCH4III ACNGT 6 cut(s) 101, 113, 227, 1944, 3198, 3364
HpyCH4IV ACGT 6 cut(s) 78, 120, 856, 2056, 2343, 2891
HpySE526I ACGT 6 cut(s) 78, 120, 856, 2056, 2343, 2891
HspAI GCGC 5 cut(s) 788, 1800, 1802, 2658, 4088
Kpn2I TCCGGA 2 cut(s) 799, 3049
Ksp22I TGATCA 5 cut(s) 886, 1887, 2329, 2524, 4234
KspAI GTTAAC 1 cut(s) 2365
LmnI GCTCC 4 cut(s) 1610, 1645, 2425, 3142
MabI ACCWGGT 2 cut(s) 2176, 2730
MaeII ACGT 6 cut(s) 78, 120, 856, 2056, 2343, 2891
MbiI CCGCTC 1 cut(s) 221
MfeI CAATTG 2 cut(s) 2004, 3074
MflI RGATCY 5 cut(s) 554, 1051, 2021, 2637, 3766
MhlI GDGCHC 4 cut(s) 1605, 2251, 2670, 3391
MlsI TGGCCA 1 cut(s) 2603
MluNI TGGCCA 1 cut(s) 2603
MlyI GAGTC 3 cut(s) 86, 1245, 2796
MmeI TCCRAC 3 cut(s) 45, 1506, 4162
Mox20I TGGCCA 1 cut(s) 2603
MroI TCCGGA 2 cut(s) 799, 3049
MroXI GAANNNNTTC 3 cut(s) 780, 807, 3686
MscI TGGCCA 1 cut(s) 2603
MslI CAYNNNNRTG 5 cut(s) 1942, 2198, 2478, 3447, 4266
Msp20I TGGCCA 1 cut(s) 2603
MspA1I CMGCKG 5 cut(s) 1628, 1861, 1957, 2542, 3673
MspI CCGG 9 cut(s) 696, 800, 1865, 2244, 3050, 3541, 4481, 4505, 4539
MunI CAATTG 2 cut(s) 2004, 3074
Mva1269I GAATGC 1 cut(s) 853
MvnI CGCG 3 cut(s) 1802, 4088, 4090
NciI CCSGG 5 cut(s) 697, 2245, 3542, 4482, 4506
NcoI CCATGG 1 cut(s) 2604
NdeI CATATG 2 cut(s) 1620, 4612
NlaIV GGNNCC 9 cut(s) 471, 603, 1647, 2100, 2175, 2248, 2249, 3768, 4537
NmuCI GTSAC 5 cut(s) 107, 113, 547, 1290, 3358
NspI RCATGY 4 cut(s) 828, 1451, 1575, 2881
NspV TTCGAA 2 cut(s) 676, 1669
OliI CACNNNNGTG 2 cut(s) 1942, 2198
PaeI GCATGC 1 cut(s) 1451
PagI TCATGA 1 cut(s) 2322
PauI GCGCGC 1 cut(s) 1800
PciI ACATGT 2 cut(s) 1571, 2877
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 4456
PctI GAATGC 1 cut(s) 853
PdmI GAANNNNTTC 3 cut(s) 780, 807, 3686
PfoI TCCNGGA 1 cut(s) 306
PleI GAGTC 3 cut(s) 86, 1245, 2796
PpsI GAGTC 3 cut(s) 86, 1245, 2796
Ppu21I YACGTR 1 cut(s) 2057
PpuMI RGGWCCY 2 cut(s) 2099, 2174
PscI ACATGT 2 cut(s) 1571, 2877
PshBI ATTAAT 2 cut(s) 2958, 3242
PsiI TTATAA 1 cut(s) 2412
Psp5II RGGWCCY 2 cut(s) 2099, 2174
PspEI GGTNACC 1 cut(s) 2180
PspFI CCCAGC 4 cut(s) 2185, 4530, 4563, 4644
PspN4I GGNNCC 9 cut(s) 471, 603, 1647, 2100, 2175, 2248, 2249, 3768, 4537
PspOMI GGGCCC 1 cut(s) 2247
PspPI GGNCC 8 cut(s) 2099, 2174, 2247, 2248, 2624, 2807, 3803, 4289
PspPPI RGGWCCY 2 cut(s) 2099, 2174
PstI CTGCAG 2 cut(s) 2547, 3736
PstNI CAGNNNCTG 2 cut(s) 4310, 4563
PsuI RGATCY 5 cut(s) 554, 1051, 2021, 2637, 3766
PteI GCGCGC 1 cut(s) 1800
PvuII CAGCTG 4 cut(s) 1628, 1861, 2542, 3673
RsaI GTAC 5 cut(s) 874, 1907, 2235, 2377, 2472
RsaNI GTAC 5 cut(s) 873, 1906, 2234, 2376, 2471
RseI CAYNNNNRTG 5 cut(s) 1942, 2198, 2478, 3447, 4266
Sau96I GGNCC 8 cut(s) 2099, 2174, 2247, 2248, 2624, 2807, 3803, 4289
ScaI AGTACT 1 cut(s) 1907
SchI GAGTC 3 cut(s) 86, 1245, 2796
SduI GDGCHC 4 cut(s) 1605, 2251, 2670, 3391
SexAI ACCWGGT 2 cut(s) 2176, 2730
SfcI CTRYAG 4 cut(s) 1468, 2543, 3635, 3732
SfuI TTCGAA 2 cut(s) 676, 1669
SinI GGWCC 4 cut(s) 2099, 2174, 2624, 2807
SmiMI CAYNNNNRTG 5 cut(s) 1942, 2198, 2478, 3447, 4266
SmlI CTYRAG 5 cut(s) 446, 1295, 1593, 2663, 3827
SmoI CTYRAG 5 cut(s) 446, 1295, 1593, 2663, 3827
SpeI ACTAGT 1 cut(s) 2033
SphI GCATGC 1 cut(s) 1451
StyI CCWWGG 4 cut(s) 2353, 2604, 3695, 4631
TaaI ACNGT 6 cut(s) 101, 113, 227, 1944, 3198, 3364
TaiI ACGT 6 cut(s) 81, 123, 859, 2059, 2346, 2894
TaqII GACCGA 1 cut(s) 3597
TatI WGTACW 4 cut(s) 1905, 2233, 2375, 2470
TseFI GTSAC 5 cut(s) 107, 113, 547, 1290, 3358
Tsp45I GTSAC 5 cut(s) 107, 113, 547, 1290, 3358
TspGWI ACGGA 6 cut(s) 2082, 2125, 3338, 3896, 4231, 4351
VneI GTGCAC 1 cut(s) 3387
VpaK11BI GGWCC 4 cut(s) 2099, 2174, 2624, 2807
VspI ATTAAT 2 cut(s) 2958, 3242
XapI RAATTY 7 cut(s) 347, 807, 964, 2506, 2749, 3299, 3685
XceI RCATGY 4 cut(s) 828, 1451, 1575, 2881
XcmI CCANNNNNNNNNTGG 1 cut(s) 4178
XmaJI CCTAGG 2 cut(s) 2353, 4631
XmiI GTMKAC 1 cut(s) 3634
XmnI GAANNNNTTC 3 cut(s) 780, 807, 3686
ZrmI AGTACT 1 cut(s) 1907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.