MD00G1108000.v1.1

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009494
Physical Location & Seq
Forward (+)
22632495 .. 22642142
9648 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1108000.v1.1.491

Sequence Viewer

Length: 4986 bp
ATGGGAAAAGCTGTTGTCTCTGACGAAGAAGATGAGGTCGAGTTCGACGAAGACGACAGGGAACCTCTTCACGGTGACGCCGTCGATGGCCAGGATGGCGGCGACGAGGACGAGGATGAGGAAGAAGAAGGGCCAGATGAATATGAGAAGGATGATTTTATAGTAGATGATGTAGACGAAGACGAGGACCAAGAGGAGGAAGAAGAGAGAGCAGACAGTGATGAAGAACGGCAGAGGAAGAAGAAAAGAAAGAAAAAAGAGGAGTATGTCCTTGATGAAGATGACTATGAGCTCTTGGAGGACAATAATGTCATTGCTCCCCGAAGGAAGGCTGGGAAATTTAAGCGTCTGAAAAAAGCTCAGAGGCATGGTGTTGGTGAGCCTGGTGGATTTTCTGATGAGGAGGAGTTTGATGGTATTGGTAAAGGTGGAAGAACTGCAGAGGAGAAACTTGAGCGCAGCTTATTTGGTCATGATGAAGGTACTCCATTTGAGGACATCGCTGAAGAGGAGGAACCAGAAGAAGCGGAAGATGATGGAGAAGTTGGTGAAGAGGATGAAATGGCAGACTTTATAGTTGATGAAGAGTTTGATGAGACTGGGACTCTTGTGAGACAGAGGAAGCTTAAGAGAAAAAGGTCTAGGCAGGCACCAGGAGTATCATCAGATGCTTTGCAGGAAGCCCATGATATATTTGGTGATGTTGATGAACTACTGCAGCTCCGTAAGCAAGGTTTGGATTCCAGTGAATGGAGGGAAAGGAAGCTTGAGGATGAATTTGAACCAATTATTCTTTCTGAAAAGTATATGACTGAGAAGGATGACCAAATTCGGGAGGTTGATGTATATGAGGAAATCACTGGTTCTTTTCCACTGGATGGAATTAGTATAGATGATGAGAGTACCTGGATATATGATCAATTAGCAAGTGGTACAATACCTTTGTTTAGCAAAACAGGCTTGGCAAATTCTATTAGCAGGGAAGATATCAACAGATTTTTGGACTTGCATCATGTGCAGAAGCTTGACATTCCTTTTATTGCTATGTATCGGAAAGAGGATTGCCCTAGTTTATTGAAAGATCCTGACCATTCTGAATTGGAAGATGTAAATCAGAATGAAAATGAGAAATCATCTATGCTAAAGTGGCACAAGGTCCTTTGGACTATTCAGGACTTGGACAGAAAATGGCTGCTGCTTCAGAAGCGAAAAAGTGCTTTGGAATCTTACTACAATAAGCGGTTTGAGGAAGAATCCCGACGTATATATGATGAGAGCAGACTTACATTGAATCAACAACTTTTTGAGTCAATCATGAAGTCTCTCAAGGCTGCAGAATCAGAAAGAGAGGTTGATGATGTTGACACCAAGTTTAACTTGCATTTCCCTCCGGGTGAGGCTGGAGTGGATGAGGGACAATATAAGAGGCCCAAGAGGAAATCTCTTTACAGTGTCTGCAGTAAGGCTGGCCTGTGGGAAGTTGCAAGCAAATTCGGTTACAGCTCTGAGCAGTTTGGACTGCAATTATCTCTAGAAAAAATGAGAATGGATGAGTTGGAGGATGCCAAGGAGACACCGGAGGAGATGGCTTCGAACTTTACATGTGCAATGTTTGAGACACCTCAAGCTGTGCTCAAAGGGGCTAGGCATATGGCAGCTGTTGAGATAAGTTGTGAGCCATGTGTTAGGAAGTATGTTCGAAGCAACTACTTGGATATCATTGAGTTATCGACAAGCCCCACGCTGGTCGGAAATGTGGCAGTAGATGCTTTTCATCAGTTCTCTGGCGTGAAGTGGTTGCAAAGAAAGCCATTAAATAAATTTGAGGATGCACAATGGCTGCTTATCCAGAAGGCCGAGGAGGAGAAGCTTCTTCAGGTTACTATTAAGTTGCCAGAAGACAGGCTAAACAAGTTGATAAGCGACTTTAATGAGTACTATCTTAGTTATGGTGTTAGTAAATCTGCACAACTATGGAATGAGCAACGGAAGTTGATATTGCAGGATGCCCTATTTACTTTTCTTCTTCCTTCAATAGAAAAAGAAGCAAGATCCTTGTTGGCTAGCAGAGCAAAAAACTGGTTACTCATGGAATATGGGAAGGTTTTGTGGAATAAAGTCTCAGTGAGACCATACCAACGAAAGGAAAATGATAATAGCTCAGACGATGAAGCTGCACCCAGGGTCATGGCTTGCTGCTGGGGCCCTGGGAAGCCAGCAACCACTTTTGTAATGTTAGATTCATCTGGTGAGGTTCTAGATGTACTTTACACGGGTTCCCTCACTTTACGCTCTCATAATGTTAATGATCAGCAGCGAAAGAAAAATGATCAAGAACGTGTTTTGAAGTTCATGACTGATCATCAACCACAAGTCGCTGTCCTAGGAGCTGTTAATTTGTCTTGTGTTCGCCTAAAAGATGATATATATGAGATCATTTTTAAGATGGTGGAGGAAAATCCTAGAGATGTTGGCCATGATATGGATGGTTTAAGTATTGTATATGGGGATGAATCTTTGGCCCGACTGTATGAAAATTCTCGGATTTCATCTGATCAGCTACCTGCACAACAAGGCATTGTCAAGAGGGCTGTGGCTCTTGGGCGTTATCTTCAAAATCCATTGGCAATGGTTGCCACACTTTGTGGACCAGGAAGAGAAATCTTATCTTGGAAACTCAATCCTTTTGAGAACTTTCTCACCCCAGATGAGAAGTACATGATGGTTGAACAGGTCATGGTAGATGTCACAAACCAGGTAGGCCTTGATATTAATTTGGCTATAAGCCATGAATGGTTATTTGCACCTTTGCAATTCATTTCTGGGCTTGGACCTAGAAAGGCAGCATCTTTGCAGAGATCGTTGGTAAGATCTGGTGCAATATTTACTCGAAAGGATTTGGTGAACCCGCATGGGCTTGGTAGAAAGGTATTTGTTAATGCATCCGGTTTCTTACGTGTTCGGCGGAGTGGGTTGGCTGCTAGCAGCAGCCAATATATTGATCTGTTGGATGATACGAGAATTCATCCAGAATACTATATAATTGCGCAAGACTTGGCCAAAGATGTTTATGATGTGGATGGTAACAATGATGATGAGGATTTGGAGATGGCTATAGAACACGTCAGGGATCGACCTGGTTATCTAAAAAACCTTGATGTTGAAGCTTATGCTAAAAGCAAGAAGCTTGAGAATAAGATACAAACATTTTATGATATAAGGAGAGAATTAATTCAGGGTTTTCAGGATTGGCGTAAACAATATGAAGAACCAAGCCAGGATGAAGAGTTCTATATGATTTCAGGTGAAACTGAGGATACCCTTGCTGAAGGAAGAATTGTGCAGGCTACAGTTCGCAGGGTGCAAGGTCAAAGGGCAATTTGTGCTCTTGAATCTGGATTAACTGGCATGCTTATGAAAGAAGATTATTCAGATGATTCAAGAGATATAGAATTGTCTGACAGACTGAACGAGGGCGACATTCTCACTTGCAAGATCAAATCCATTCAAAAGAATAGGTATCAAGTTTTCCTAAGTTGTAGAGAGAGTGAGATGAGAAATAACCGATATCAGAACACTCAGAATCTTGATACCTATTACCATGAAGACCGAAGAAGTTTACAGAGTGAGCAAGATAAAGCTCATAAGGAGAAAGAGCTTGCGAAGAAGCATTTCAAGCCAAGGATGATTGTTCATCCTCGCTTCCAAAATATAACTGCAGATGAAGCATTGAAGTTCTTGTCTGATAAGGATCCTGGGGAAAGTGTTATTCGTCCTAGTTCCCGGGGGCCTTCGTATTTAACTTTGACTCTCAAGGTTTATGATGGAGTGTATGCTCACAAAGACATTGTTGAGGGTGGAAAGGATCATAAGGATATCACGAGCTTACTCCGCATTGGGAAGACCTTGAAAATTGGGGAGGACACCTTTGAGGATTTAGATGAGGTGATGGATCGTTATGTTGATCCCTTGGTAGCTCACTTGAAGGCAATTCTAAATTACCGCAAGTTCAGGAAGGGAACAAAACAAGAGGTTGATGAACTTTTGAAGATGGAGAAATTGGAGTATCCAATGAGGATTGTTTATTCTTTTGGGATTTCACATGAACATCCAGGCACTTTCATCTTGACGTATATACGCAGTACAAATCCACACCATGAGTATATTGGCCTATATCCGAAGGGATTCAAATTCAGAAAAAGGATGTTTGAGGACATTGATCGGCTTTTAGCATATTTTCAGCGCCATATTGATGACCCTCAGCATGAATCAGGACCATCTATTAGATCAGTCGCTGCTATGGTGCCGATACGAAGCCCTGCAGCTGGGGGTTCTTCAGGGGCATCTGTGGGCAGCGGCTGGGGTGGATCAACTAATGAGGGTGGTTGGAGAGGCCAGTCATTTGATAGGGACCGGTCATCTACACCGAGTTCACGGACAGGTCGAAATGATCATAGAAATGGCGGTAGTCGTGATGCGCATCCAAGCGGCCTCCCTAGACCTTATGGTGGGCGAGGTCATTGTCGAGGAGGATATAACAACAGAGGAAATAGCACTAGCAATGAGAGGCAGGATTCTGGTAATGATGCTCCTGCGTGGGGTTCAGATTCCAAGGATGCGGATGATGGTTTGGGCAATTTTCCAGGTGCAAAGGTCCAAAACTCGCCTGGAAGGGAAGCTTTCCCTGGTGGTTGGGGTGCTGGTGGAAGTGGCAGTGGAGGTGGTGGCAGTAGCTGGGGCAGTGGAGCTGGCTCCAGTGGAAATGATGCGTGGGGTCAAGGAATTGGCGGTGCAGGAGGATGGGGTGTGTCTGGTGCAAATGATGCAGCTGCAGCTGCTGATAATGGGACTTCAGGATGGGGTTCTAGCGCCAAGAAAAAAGGTTCTGGTGCTGGAGATTTGGGATGGGGTTCAGAAACCAAAAGAAGTACCCCGTCCCAATCTGGAAACAGATGGTCTGGGACTGACGGCGGTGGTGGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

1662

Amino Acids

187.65

Weight (kDa)

4.98

Isoelectric Point (pI)

45.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT6_acidic PF14632 35 - 122 8.4e-16 Acidic N-terminal SPT6
HTH_44 PF14641 297 - 413 2.2e-06 Helix-turn-helix DNA-binding domain of SPT6
YqgF PF14639 726 - 882 5e-16 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 886 - 987 2.1e-21 Helix-hairpin-helix motif
Spt6_S1 PF21710 1097 - 1184 1.4e-10 Spt6, S1/OB domain
SH2_2 PF14633 1200 - 1416 7.3e-69 SH2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
Acc16I TGCGCA 2 cut(s) 3048, 4479
Acc36I ACCTGC 1 cut(s) 2572
AccB1I GGYRCC 2 cut(s) 649, 4303
AccB7I CCANNNNNTGG 3 cut(s) 750, 878, 2482
AccI GTMKAC 1 cut(s) 174
AclWI GGATC 9 cut(s) 1076, 2046, 3138, 3746, 3759, 3873, 3959, 3960, 4375
AcoI YGGCCR 3 cut(s) 88, 2473, 3057
AcsI RAATTY 9 cut(s) 338, 776, 828, 967, 1490, 1820, 2536, 3021, 4190
AcuI CTGAAG 6 cut(s) 525, 1184, 1859, 3348, 4320, 4836
AcyI GRCGYC 1 cut(s) 78
AdeI CACNNNGTG 1 cut(s) 2645
AfaI GTAC 8 cut(s) 484, 904, 934, 1937, 2265, 2717, 4144, 4930
AflII CTTAAG 1 cut(s) 626
AflIII ACRYGT 4 cut(s) 1601, 2338, 2956, 3121
AgeI ACCGGT 1 cut(s) 4413
AjiI CACGTC 1 cut(s) 3124
AjuI GAANNNNNNNTTGG 2 cut(s) 778, 810
AloI GAACNNNNNNTCC 2 cut(s) 3272, 3304
Alw21I GWGCWC 3 cut(s) 294, 1635, 3388
Alw26I GTCTC 8 cut(s) 22, 590, 607, 1326, 1565, 1610, 2122, 2125
AlwI GGATC 9 cut(s) 1076, 2046, 3138, 3746, 3759, 3873, 3959, 3960, 4375
AlwNI CAGNNNCTG 5 cut(s) 1455, 4295, 4359, 4734, 4838
Ama87I CYCGRG 1 cut(s) 3783
ApaI GGGCCC 1 cut(s) 2207
ApoI RAATTY 9 cut(s) 338, 776, 828, 967, 1490, 1820, 2536, 3021, 4190
AseI ATTAAT 2 cut(s) 2772, 3230
AsiGI ACCGGT 1 cut(s) 4413
Asp700I GAANNNNTTC 4 cut(s) 66, 3231, 3671, 4184
AspA2I CCTAGG 1 cut(s) 2383
AspLEI GCGC 5 cut(s) 459, 3049, 4245, 4480, 4871
AsuC2I CCSGG 3 cut(s) 1392, 3784, 3785
AsuII TTCGAA 2 cut(s) 1592, 1699
AsuNHI GCTAGC 2 cut(s) 2063, 2981
AvaI CYCGRG 1 cut(s) 3783
AvaII GGWCC 7 cut(s) 187, 1156, 2648, 2831, 4274, 4411, 4654
AvrII CCTAGG 1 cut(s) 2383
BaeGI GKGCMC 1 cut(s) 2207
BalI TGGCCA 3 cut(s) 90, 2475, 3059
BamHI GGATCC 1 cut(s) 3751
BanI GGYRCC 2 cut(s) 649, 4303
BanII GRGCYC 2 cut(s) 294, 2207
BauI CACGAG 1 cut(s) 3880
BbsI GAAGAC 5 cut(s) 57, 186, 1905, 3612, 3908
Bbv12I GWGCWC 3 cut(s) 294, 1635, 3388
BbvCI CCTCAGC 1 cut(s) 4260
BceAI ACGGC 3 cut(s) 65, 245, 4984
BcgI CGANNNNNNTGC 2 cut(s) 2156, 2190
BciVI GTATCC 2 cut(s) 3310, 4077
BclI TGATCA 6 cut(s) 916, 2308, 2329, 2359, 2554, 4450
BcnI CCSGG 3 cut(s) 1392, 3784, 3785
BcoDI GTCTC 8 cut(s) 22, 590, 607, 1326, 1565, 1610, 2122, 2125
BfmI CTRYAG 9 cut(s) 438, 716, 1332, 1456, 3114, 3348, 3717, 4320, 4830
BfoI RGCGCY 2 cut(s) 4246, 4872
BfrI CTTAAG 1 cut(s) 626
BfuAI ACCTGC 1 cut(s) 2572
BfuI GTATCC 2 cut(s) 3310, 4077
BglI GCCNNNNNGGC 1 cut(s) 96
BglII AGATCT 1 cut(s) 2870
BlnI CCTAGG 1 cut(s) 2383
BmcAI AGTACT 1 cut(s) 1937
Bme18I GGWCC 7 cut(s) 187, 1156, 2648, 2831, 4274, 4411, 4654
BmeT110I CYCGRG 1 cut(s) 3783
BmgBI CACGTC 1 cut(s) 3124
BmrI ACTGGG 1 cut(s) 609
BmtI GCTAGC 2 cut(s) 2067, 2985
BmuI ACTGGG 1 cut(s) 609
BpiI GAAGAC 5 cut(s) 57, 186, 1905, 3612, 3908
BplI GAGNNNNNCTC 2 cut(s) 1426, 1458
BpmI CTGGAG 3 cut(s) 1422, 4738, 4914
Bpu10I CCTNAGC 1 cut(s) 4260
Bpu14I TTCGAA 2 cut(s) 1592, 1699
BpuEI CTTGAG 6 cut(s) 473, 788, 1310, 1608, 3209, 3797
BpuMI CCSGG 3 cut(s) 1392, 3784, 3785
BsaAI YACGTR 1 cut(s) 2957
BsaBI GATNNNNATC 3 cut(s) 1110, 3750, 4479
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 2122
BsaWI WCCGGW 3 cut(s) 1576, 2945, 4413
BsaXI ACNNNNNCTCC 2 cut(s) 705, 735
Bse118I RCCGGY 1 cut(s) 4413
Bse1I ACTGG 8 cut(s) 604, 744, 865, 879, 2084, 3409, 4396, 4755
Bse3DI GCAATG 4 cut(s) 312, 1614, 2634, 4567
Bse8I GATNNNNATC 3 cut(s) 1110, 3750, 4479
BseJI GATNNNNATC 3 cut(s) 1110, 3750, 4479
BseMI GCAATG 4 cut(s) 312, 1614, 2634, 4567
BseMII CTCAG 8 cut(s) 374, 804, 1497, 2136, 2175, 3303, 3593, 4274
BseNI ACTGG 8 cut(s) 604, 744, 865, 879, 2084, 3409, 4396, 4755
BseSI GKGCMC 1 cut(s) 2207
BseYI CCCAGC 5 cut(s) 332, 2199, 4325, 4359, 4734
BsgI GTGCAG 6 cut(s) 1037, 1950, 2160, 2550, 3362, 4812
BshNI GGYRCC 2 cut(s) 649, 4303
BshTI ACCGGT 1 cut(s) 4413
BsiHKAI GWGCWC 3 cut(s) 294, 1635, 3388
BsiHKCI CYCGRG 1 cut(s) 3783
BsiSI CCGG 5 cut(s) 1391, 1577, 2946, 3784, 4414
BslFI GGGAC 6 cut(s) 616, 1428, 4424, 4861, 4921, 4975
BsmAI GTCTC 8 cut(s) 22, 590, 607, 1326, 1565, 1610, 2122, 2125
BsmFI GGGAC 6 cut(s) 616, 1428, 4424, 4861, 4921, 4975
Bso31I GGTCTC 1 cut(s) 2122
BsoBI CYCGRG 1 cut(s) 3783
Bsp119I TTCGAA 2 cut(s) 1592, 1699
Bsp120I GGGCCC 1 cut(s) 2203
Bsp1286I GDGCHC 4 cut(s) 294, 1635, 2207, 3388
BspCNI CTCAG 8 cut(s) 373, 805, 1498, 2135, 2174, 3304, 3592, 4273
BspHI TCATGA 3 cut(s) 472, 1314, 2352
BspMAI CTGCAG 7 cut(s) 442, 720, 1336, 1460, 3721, 4324, 4834
BspMI ACCTGC 1 cut(s) 2572
BspOI GCTAGC 2 cut(s) 2067, 2985
BspPI GGATC 9 cut(s) 1076, 2046, 3138, 3746, 3759, 3873, 3959, 3960, 4375
BspT104I TTCGAA 2 cut(s) 1592, 1699
BspT107I GGYRCC 2 cut(s) 649, 4303
BspTI CTTAAG 1 cut(s) 626
BspTNI GGTCTC 1 cut(s) 2122
BsrDI GCAATG 4 cut(s) 312, 1614, 2634, 4567
BsrFI RCCGGY 1 cut(s) 4413
BsrI ACTGG 8 cut(s) 604, 744, 865, 879, 2084, 3409, 4396, 4755
BssAI RCCGGY 1 cut(s) 4413
BssNI GRCGYC 1 cut(s) 78
BssSI CACGAG 1 cut(s) 3880
BssT1I CCWWGG 5 cut(s) 1566, 2383, 3680, 3969, 4611
Bst2BI CACGAG 1 cut(s) 3880
Bst4CI ACNGT 5 cut(s) 74, 218, 1451, 2529, 3352
Bst6I CTCTTC 7 cut(s) 72, 198, 501, 546, 579, 2650, 3279
BstACI GRCGYC 1 cut(s) 78
BstAFI CTTAAG 1 cut(s) 626
BstAPI GCANNNNNTGC 5 cut(s) 1015, 1766, 2633, 3383, 4823
BstBAI YACGTR 1 cut(s) 2957
BstBI TTCGAA 2 cut(s) 1592, 1699
BstH2I RGCGCY 2 cut(s) 4246, 4872
BstHHI GCGC 5 cut(s) 459, 3049, 4245, 4480, 4871
BstMAI GTCTC 8 cut(s) 22, 590, 607, 1326, 1565, 1610, 2122, 2125
BstNSI RCATGY 2 cut(s) 1605, 3412
BstSFI CTRYAG 9 cut(s) 438, 716, 1332, 1456, 3114, 3348, 3717, 4320, 4830
BstSLI GKGCMC 1 cut(s) 2207
BstV2I GAAGAC 5 cut(s) 57, 186, 1905, 3612, 3908
BstX2I RGATCY 4 cut(s) 1081, 2051, 2870, 3751
BstXI CCANNNNNNTGG 1 cut(s) 2188
BstYI RGATCY 4 cut(s) 1081, 2051, 2870, 3751
BsuI GTATCC 2 cut(s) 3310, 4077
BtgZI GCGATG 1 cut(s) 484
BtrI CACGTC 1 cut(s) 3124
BtsI GCAGTG 2 cut(s) 4720, 4747
BtsIMutI CAGTG 9 cut(s) 223, 751, 858, 872, 1456, 2130, 4720, 4747, 4762
BveI ACCTGC 1 cut(s) 2572
CaiI CAGNNNCTG 5 cut(s) 1455, 4295, 4359, 4734, 4838
CciI TCATGA 3 cut(s) 472, 1314, 2352
CfoI GCGC 5 cut(s) 459, 3049, 4245, 4480, 4871
Cfr10I RCCGGY 1 cut(s) 4413
Cfr9I CCCGGG 1 cut(s) 3783
CseI GACGC 2 cut(s) 86, 335
CsiI ACCWGGT 2 cut(s) 2754, 3136
Csp6I GTAC 8 cut(s) 483, 903, 933, 1936, 2264, 2716, 4143, 4929
CspAI ACCGGT 1 cut(s) 4413
CviQI GTAC 8 cut(s) 483, 903, 933, 1936, 2264, 2716, 4143, 4929
DraIII CACNNNGTG 1 cut(s) 2645
DrdI GACNNNNNNGTC 1 cut(s) 308
DseDI GACNNNNNNGTC 1 cut(s) 308
EaeI YGGCCR 3 cut(s) 88, 2473, 3057
Eam1104I CTCTTC 7 cut(s) 72, 198, 501, 546, 579, 2650, 3279
EarI CTCTTC 7 cut(s) 72, 198, 501, 546, 579, 2650, 3279
EciI GGCGGA 1 cut(s) 2980
Ecl136II GAGCTC 1 cut(s) 292
Eco130I CCWWGG 5 cut(s) 1566, 2383, 3680, 3969, 4611
Eco147I AGGCCT 1 cut(s) 2763
Eco24I GRGCYC 2 cut(s) 294, 2207
Eco31I GGTCTC 1 cut(s) 2122
Eco32I GATATC 4 cut(s) 988, 1717, 3569, 3877
Eco47I GGWCC 7 cut(s) 187, 1156, 2648, 2831, 4274, 4411, 4654
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 6 cut(s) 525, 1184, 1859, 3348, 4320, 4836
Eco88I CYCGRG 1 cut(s) 3783
EcoICRI GAGCTC 1 cut(s) 292
EcoO109I RGGNCCY 4 cut(s) 1156, 2203, 2204, 3788
EcoRI GAATTC 1 cut(s) 3021
EcoRV GATATC 4 cut(s) 988, 1717, 3569, 3877
EcoT14I CCWWGG 5 cut(s) 1566, 2383, 3680, 3969, 4611
EcoT22I ATGCAT 1 cut(s) 2944
EcoT38I GRGCYC 2 cut(s) 294, 2207
ErhI CCWWGG 5 cut(s) 1566, 2383, 3680, 3969, 4611
FalI AAGNNNNNCTT 4 cut(s) 1361, 1393, 4663, 4695
FaqI GGGAC 6 cut(s) 616, 1428, 4424, 4861, 4921, 4975
FauI CCCGC 1 cut(s) 2916
FauNDI CATATG 1 cut(s) 1650
FbaI TGATCA 6 cut(s) 916, 2308, 2329, 2359, 2554, 4450
FblI GTMKAC 1 cut(s) 174
FriOI GRGCYC 2 cut(s) 294, 2207
FspAI RTGCGCAY 1 cut(s) 4479
FspI TGCGCA 2 cut(s) 3048, 4479
GlaI GCGC 5 cut(s) 458, 3048, 4244, 4479, 4870
GsaI CCCAGC 5 cut(s) 336, 2203, 4329, 4363, 4738
GsuI CTGGAG 3 cut(s) 1422, 4738, 4914
HaeII RGCGCY 2 cut(s) 4246, 4872
HapII CCGG 5 cut(s) 1391, 1577, 2946, 3784, 4414
HgaI GACGC 2 cut(s) 86, 335
HhaI GCGC 5 cut(s) 459, 3049, 4245, 4480, 4871
Hin1I GRCGYC 1 cut(s) 78
Hin6I GCGC 5 cut(s) 457, 3047, 4243, 4478, 4869
HinP1I GCGC 5 cut(s) 457, 3047, 4243, 4478, 4869
HincII GTYRAC 1 cut(s) 1363
HindII GTYRAC 1 cut(s) 1363
HindIII AAGCTT 7 cut(s) 623, 764, 1022, 1868, 3165, 3185, 4677
HpaII CCGG 5 cut(s) 1391, 1577, 2946, 3784, 4414
Hpy166II GTNNAC 7 cut(s) 175, 1363, 2648, 2905, 3257, 3620, 4433
Hpy8I GTNNAC 7 cut(s) 175, 1363, 2648, 2905, 3257, 3620, 4433
Hpy99I CGWCG 4 cut(s) 50, 86, 107, 1263
HpyCH4III ACNGT 5 cut(s) 74, 218, 1451, 2529, 3352
HpyCH4IV ACGT 5 cut(s) 1261, 2338, 2956, 3123, 4130
HpySE526I ACGT 5 cut(s) 1261, 2338, 2956, 3123, 4130
Hsp92I GRCGYC 1 cut(s) 78
HspAI GCGC 5 cut(s) 457, 3047, 4243, 4478, 4869
Ksp22I TGATCA 6 cut(s) 916, 2308, 2329, 2359, 2554, 4450
LmnI GCTCC 6 cut(s) 322, 726, 2387, 4594, 4745, 4757
MabI ACCWGGT 2 cut(s) 2754, 3136
MaeII ACGT 5 cut(s) 1261, 2338, 2956, 3123, 4130
MaeIII GTNAC 6 cut(s) 74, 1496, 1879, 2082, 2746, 3083
MflI RGATCY 4 cut(s) 1081, 2051, 2870, 3751
MhlI GDGCHC 4 cut(s) 294, 1635, 2207, 3388
MlsI TGGCCA 3 cut(s) 90, 2475, 3059
MluNI TGGCCA 3 cut(s) 90, 2475, 3059
MlyI GAGTC 3 cut(s) 598, 1316, 3802
MmeI TCCRAC 4 cut(s) 1536, 1729, 2988, 4367
Mox20I TGGCCA 3 cut(s) 90, 2475, 3059
Mph1103I ATGCAT 1 cut(s) 2944
MroXI GAANNNNTTC 4 cut(s) 66, 3231, 3671, 4184
MscI TGGCCA 3 cut(s) 90, 2475, 3059
MslI CAYNNNNRTG 4 cut(s) 1972, 3413, 4251, 4458
Msp20I TGGCCA 3 cut(s) 90, 2475, 3059
MspA1I CMGCKG 5 cut(s) 1658, 4325, 4356, 4829, 4835
MspCI CTTAAG 1 cut(s) 626
MspI CCGG 5 cut(s) 1391, 1577, 2946, 3784, 4414
NciI CCSGG 3 cut(s) 1392, 3784, 3785
NdeI CATATG 1 cut(s) 1650
NheI GCTAGC 2 cut(s) 2063, 2981
NmeAIII GCCGAG 1 cut(s) 1882
NmuCI GTSAC 2 cut(s) 74, 2746
NsbI TGCGCA 2 cut(s) 3048, 4479
NsiI ATGCAT 1 cut(s) 2944
NspI RCATGY 2 cut(s) 1605, 3412
NspV TTCGAA 2 cut(s) 1592, 1699
PaeI GCATGC 1 cut(s) 3412
PagI TCATGA 3 cut(s) 472, 1314, 2352
PasI CCCWGGG 2 cut(s) 2181, 2207
PceI AGGCCT 1 cut(s) 2763
PciI ACATGT 1 cut(s) 1601
PcsI WCGNNNNNNNCGW 4 cut(s) 45, 51, 78, 4441
PdmI GAANNNNTTC 4 cut(s) 66, 3231, 3671, 4184
PflFI GACNNNGTC 1 cut(s) 80
PflMI CCANNNNNTGG 3 cut(s) 750, 878, 2482
PinAI ACCGGT 1 cut(s) 4413
PleI GAGTC 3 cut(s) 598, 1315, 3802
PpsI GAGTC 3 cut(s) 598, 1315, 3802
Ppu21I YACGTR 1 cut(s) 2957
PpuMI RGGWCCY 1 cut(s) 1156
PscI ACATGT 1 cut(s) 1601
PshBI ATTAAT 2 cut(s) 2772, 3230
Psp124BI GAGCTC 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 1156
PspFI CCCAGC 5 cut(s) 332, 2199, 4325, 4359, 4734
PspOMI GGGCCC 1 cut(s) 2203
PspPPI RGGWCCY 1 cut(s) 1156
PstI CTGCAG 7 cut(s) 442, 720, 1336, 1460, 3721, 4324, 4834
PstNI CAGNNNCTG 5 cut(s) 1455, 4295, 4359, 4734, 4838
PsuI RGATCY 4 cut(s) 1081, 2051, 2870, 3751
PsyI GACNNNGTC 1 cut(s) 80
PvuII CAGCTG 4 cut(s) 1658, 4325, 4829, 4835
RsaI GTAC 8 cut(s) 484, 904, 934, 1937, 2265, 2717, 4144, 4930
RsaNI GTAC 8 cut(s) 483, 903, 933, 1936, 2264, 2716, 4143, 4929
RseI CAYNNNNRTG 4 cut(s) 1972, 3413, 4251, 4458
SacI GAGCTC 1 cut(s) 294
ScaI AGTACT 1 cut(s) 1937
SchI GAGTC 3 cut(s) 598, 1316, 3802
SduI GDGCHC 4 cut(s) 294, 1635, 2207, 3388
SexAI ACCWGGT 2 cut(s) 2754, 3136
SfcI CTRYAG 9 cut(s) 438, 716, 1332, 1456, 3114, 3348, 3717, 4320, 4830
SfuI TTCGAA 2 cut(s) 1592, 1699
SinI GGWCC 7 cut(s) 187, 1156, 2648, 2831, 4274, 4411, 4654
SmaI CCCGGG 1 cut(s) 3785
SmiMI CAYNNNNRTG 4 cut(s) 1972, 3413, 4251, 4458
SmlI CTYRAG 7 cut(s) 452, 626, 767, 1325, 1623, 3188, 3812
SmoI CTYRAG 7 cut(s) 452, 626, 767, 1325, 1623, 3188, 3812
SphI GCATGC 1 cut(s) 3412
SseBI AGGCCT 1 cut(s) 2763
SspI AATATT 1 cut(s) 2883
SstI GAGCTC 1 cut(s) 294
StuI AGGCCT 1 cut(s) 2763
StyI CCWWGG 5 cut(s) 1566, 2383, 3680, 3969, 4611
TaaI ACNGT 5 cut(s) 74, 218, 1451, 2529, 3352
TaiI ACGT 5 cut(s) 1264, 2341, 2959, 3126, 4133
TaqII GACCGA 1 cut(s) 3624
TatI WGTACW 4 cut(s) 1935, 2263, 2715, 4142
TauI GCSGC 3 cut(s) 102, 4359, 4491
TscAI CASTG 9 cut(s) 223, 751, 865, 879, 1456, 2130, 4720, 4747, 4762
TseFI GTSAC 2 cut(s) 74, 2746
Tsp45I GTSAC 2 cut(s) 74, 2746
TspGWI ACGGA 3 cut(s) 713, 2002, 4450
TspMI CCCGGG 1 cut(s) 3783
TspRI CASTG 9 cut(s) 223, 751, 865, 879, 1456, 2130, 4720, 4747, 4762
Tth111I GACNNNGTC 1 cut(s) 80
Van91I CCANNNNNTGG 3 cut(s) 750, 878, 2482
Vha464I CTTAAG 1 cut(s) 626
VpaK11BI GGWCC 7 cut(s) 187, 1156, 2648, 2831, 4274, 4411, 4654
VspI ATTAAT 2 cut(s) 2772, 3230
XapI RAATTY 9 cut(s) 338, 776, 828, 967, 1490, 1820, 2536, 3021, 4190
XbaI TCTAGA 2 cut(s) 1531, 2257
XceI RCATGY 2 cut(s) 1605, 3412
XcmI CCANNNNNNNNNTGG 4 cut(s) 692, 2483, 4163, 4664
XmaI CCCGGG 1 cut(s) 3783
XmaJI CCTAGG 1 cut(s) 2383
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 4 cut(s) 66, 3231, 3671, 4184
ZrmI AGTACT 1 cut(s) 1937
Zsp2I ATGCAT 1 cut(s) 2944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.