FvH4_1g29220

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009494
Physical Location & Seq
Forward (+)
21981278 .. 21989401
8124 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29220.t1

Sequence Viewer

Length: 4863 bp
ATGGGCAAAGCTGTCATCTCCGACGACGAGGATGAGGTCGAGCTCGACGAGGACGATAGGGAACCGATCGACGGGGACACAGTCGACCGTGACGGCGCCGACGAAGATGAAGATGAAGAAGACGAAGGGCCAGATGAATATGAGAAGGACGATTTTATCGTAGACGATGTTGATGAAGAGGATGAGCAGGAGGAAGAAGAGAAGGCGGACAGTGATGAGGAGAGGCAGAAGAAGAAGAAAAGAAAGAAGAAAGAGGAATATGTTCTTGATGAAGATGACTATGAGCTCCTGGAGGACAATAATGTGATAGCCCCTCGCAGAAAGGGACAATTTAAGCGGCTGAAAAAAGCTCAGAGGCATGCTCAGGGGGATGTTGGCGGATTATCTGATGAGGAGGAGTTTCATGGTACTGGTAAAGGGGGAAGAACTGCAGAGGAGAAGCTTAAGTTCTCATTATTTGGTGATGAGGAAGGTCCTCCACTTGAGGACATTGCTGAAGAGGAGGAACCAGCTGAAGCGGAAGATGATGGAGAAGACGAAATGGCAGACTTTATAGTTGATGAAGAGTTTGATGAGGCTGGTGTTCCTGTTAGACAGAAGAAGCTTAAGAAAAAGAAATCTAGGCAGGCAGCAGGGGTATCTTCATCTGCTCTGCAGGAAGCCCATGATATATTCGGTGATGTTGATGTTTTTATTCGGCAACGTCAGCAAGGTTTGGATTTGAGTGAATGGAAGGAAAAAAAGCTTGAGGATGAATTTGAACCGATTGTTCTTTCGGAAAAGTATATGACAATGAAGGATGACCAGATTCGAGAGATTGATGTCCCAGAAAGAATTCAGGTATATGAAGAAAGCAGTGGTTTTCTTCCTCTTGATGAAAAGAGCATAGATGATGAGAGTACCTGGATATTCAATCAATTTGCAAGTGGTACAGTTCCTTTTTTTGGCAAGACAGGCCTGGGGAATTTTATAAGCAGGGACGACATCATCGGATTCTTGAACTTGCACCATGTGCAGAAGTTGGATGTTCCTTTTATTGCAATGTATCGGAAGGAGGAATGCCCGAGTATATTGAAAGATCCGGAACACATTGACATGGATGATCAAAATGAGAAAGCATCCACACTAAAGTGGCACAAGGTACTTTGGTCAATTCAGGACTTGCACAGAAAATGGCTGCTGCTTCAGAAGCGGAAAAGTGCACTGCAATCTTACTATGAAAAGCGGTTTGATGAAGAATCTCGACGCATATATGATGAGACCAGACTAACTTTAAATCAGCAACTTTTTGAATCAATTATGAAGTCACTAAAGGCTGCAGAATCAGAGAGAGAAGTTGATGATGTGGACACCAAATTTAACCTACATTTCCCTGCTGGTGAGATAGGTGTGGATGAGGGCCAATATAAGAGGCCTAAGAGGAAGTCACTTTATAGTACCTGCAGTAAGGCTGGCTTGTGGGAGGTTGCCAGCAAGTTTGGTTACACCTCTGAACAGTTTGGTTTGCAGTTATCACTAGAGGAAATGAGAATGGATGAGCTGGAGGATGCGAAAGAGACTCCCGAGGAATTGTCTTCAAACTTCACGTGTGCAATGTTTGAGACGCCCCAAGAAGTACTCAAAGGAGCCAGGCATATGGCTGCTGTTGAGATAAGCTGTGAGCCGTGTGTCCGTAAGTATGTTCGGAGCAACTACTTGGATATGGTTGAGTTGTCCACAAGCCCAACACCAGACGGAAATGCTGCAATTGATGCTTCGCATCAGTTTGCAGGGGTGAAGTGGTTGCAAAGAAAGCCATTAAATAGATTTGAGGATGCACAATGGCTGCTTATCCAGAAGGCTGAAGAGGAGAAGCTTCTTCAAGTTACTATTAAGCTACCGGAAGACAGATTAAACAAGTTGATGAGTGACTTTAATGAGTACTATCTTAGTGATGGTGTTAGTAAATCTGCACAACTGTGGAATGAGCAGCGAAAGTTGATATTGCAGGATGCTCTTTTCCGTTTTCTTCTACCTTCAATGGAGAAAGAGGCAAGAGCCTTGTTGACTAGTAGGGCAAAAAGCTGGTTACTGACGGAATATGGAAAGGTTTTGTGGAATAAAGTCTCTGTGGGACCATATCAACGGAAAGAGAATGACATTAGTACAGACGATGAAGCTGCACCTCGAGTCATGGCTTGCTGTTGGGGCCCTGGGAAGCCAGCAACCACTTTTGTTATGTTAGATTCATCGGGGGAGGTGCTAGATGTGCTTTACACGGGGTCTCTCACCTTACGCTCCCAGAACGTTAATGATCAGCAACGGAAGAAGAATGATCAAGAACGTGTATTGAAGTTCATGACTGAACACCAACCACATGTGGCTGTTCTTGGAGCTGCTAATTTGTCTTGTGTTCGCCTTAAAGAGGATATTTACGAGATAATTTTTAAGATGGTGGAGGAGAATCCTAGAGATGTTGGACATGATATGGATGGTCTAACCATTGTCTATGGGGATGAATCTTTGGCTCGCCTGTTTGAAAATTCTCGCATTTCATCTGATCAGCTACCTGCACAATCAGGCATTGTGAAGCGTGCTGTGGCTCTTGGGCGTTATCTTCAAAATCCTTTGGCAATGGTTGCCACTCTATGTGGACCAGGAAGAGAAATCTTATCTTGGAAACTCAATCCTATGGAGAACTTTCTCACCCAGGATGAGAAGTATTCAATGATTGAACAGGTGATGGTAGATGTTACAAACCAGGTAGGCCTCGACATAAATTTGTCTATAAGCCATGAATGGTTATTTGCACCTTTGCAATTCATTTCTGGGCTTGGACCTAGAAAGGCAGCATTTTTGCAGCGATCATTGGTAAGATCTGGTGCAATATTTACTCGGAAGGACTTTTTGACAGCACATGGGTTAAGTAAAAAGGTGTTCGTTAATGCAGTTGGATTCTTGCGTGTTCGGCGCAGTGGGTTGGCTGCTAGCAGCAGCCAATTTATCGATCTGTTGGATGATACAAGAATTCATCCAGAATCCTATATTCTTGCACAAGAATTAGCCAAAGATGTTTTTGAAGTTGATGGTGGCAATGATGACGAGGATGCTATGGAGATGGCTATAGAACATGTTAGGGATCGACCTGCTTATCTAAAAAGTCTTGATGTCGAAGCATATGCCAAAAGCAAAGAGCGTGAGAATAAGATACAAACATTTTATGACATCAAAAGAGAATTGATACAGGGTTTTCAGGATTGGCGTAAGAAATATGAAGAACTAAGTCAAGATGAAGAATTCTATATGATTTCAGGTGAAACTGAAGATACCCTTGCTGAAGGAAGAATTGTACAGGCGACGGTTCGAAGGGTGCAAGCTCAAAAAGCGATTTGTGCACTTGAATCTGGACTGACTGGTATGCTTACGAAAGAAGATTTTTCAGATGATTCGAGGGACATTTCTGACTTATCTGAGAGACTGAATGAGGGTGACATTCTCACTTGCAAGATCAAATCTATTCAAAAGAATAGGTATCTGGTGTTCCTAGTTTGTAGAGAGAGTGAAATGAGACATAACCGCGATCAATATATTAAGAATCTCGATACCTATTTCCATGAAGGCAGGAGAAGTCTACAGACTGAGCAAGAAAAAGCTCGTAAGGAGAAGGAGCTGGCGAAAAAACATTTCAAGCCTAGGATGATTGTTCATCCTCGATTCCAGAATATTACGGCTGATGAAGCAATGAAGTTCTTATCTGATAAAGATCCTGGGGAAAGTATTATTCGTCCTAGTTCTCGTGGGCCTTCATATTTGACCTTAACCCTCAAAGTTTATGATGGAGTTTATGCTCACAAAGATGTAGTTGAAGGTGGAAAGGAACACAAGGATATCACCAGCTTACTCCGTATTGGAAAGACCCTGAAAATAGGAGAGGACACTTTTGAGGATTTGGATGAGGTAATGGATCGTTACGTTGATCCCCTGGTGTCTCACTTGAAAGCAATGCTAAATTATCGCAAATTCCGACGGGGCACGAAAGCTGAAGTTGATGAACTTTTGAAGATTGAAAAATTGGAGTTTCCTATGAGGATTGTTTATTGTTTCGGGATATCACATGAGCATCCAGGGACTTTCATTCTGACCTATATACGGAGTACAAATCCACACCATGAGTATGTTGGGCTGTATCCGAAGGGGTTCAAGTTCAGAAAAAAGATGTTTGATAGCATTGATCGGCTTGTAGCTTACTTCCAGAAATACATTGATGAGCCTCAACATGAGTCCGGACAATCTATAAGGTCAGTTGCTGCTATGGTGCCTATGCGAAGCCCTGCAACTGCGGGTTCTTCAGGTGCATCCGCAGGCAGTGGATGGGGTGGTATAAACAATGAGGGTGGTTGGAGTGGCCAGTCATTTGACAGGGATAGGTCTTCTACACCTAGTTCCCGAACAGGTAGAAATGATTACAGAAATGGTGGCGGTCGAGATGGACATCCAAGTGGCCTACCTAGACCATATGGGGGGCGTGGTCGTGGTCGAGTAACATATAATGATACATGGGGTTCAGATGCGAAGGATGGCAATGATGGCTTGGGCAACTTTCCAGGTGCAAAGGTTCAAAACTCACCTGGCAGGGAAGCTTTCCCAGGTGTCTGGGGTACAGATGGAAGTAGCAGTGGAGGCGGCAGTGGCTGGGCTGGTGGTGGTGCAAGTGGTTGGGGTAAAGAAAGTGGTGGTGGCACAGGAGGATGGGGTGGGGGTGCAAATGATGCTGCCCCTGATAATGAGCATTCAGGATGGGGTTCTGACTCAAAAAAGAAGGGTTCAAGCGATGGAACTTCAGGATGGGGCTCGCAGAGTGGAGGTGGGGGTGGTGGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

1621

Amino Acids

183.26

Weight (kDa)

5.01

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT6_acidic PF14632 34 - 119 2e-15 Acidic N-terminal SPT6
YqgF PF14639 721 - 877 2.1e-16 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 881 - 982 7.3e-20 Helix-hairpin-helix motif
HHH_9 PF17674 997 - 1081 7.4e-06 HHH domain
Spt6_S1 PF21710 1093 - 1180 1.2e-08 Spt6, S1/OB domain
SH2_2 PF14633 1207 - 1412 6.2e-69 SH2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 971
Acc36I ACCTGC 3 cut(s) 1448, 2557, 3133
AccB1I GGYRCC 2 cut(s) 95, 4294
AccI GTMKAC 3 cut(s) 84, 162, 3610
AccII CGCG 1 cut(s) 3558
AccIII TCCGGA 2 cut(s) 1081, 4262
AclI AACGTT 1 cut(s) 2286
AclWI GGATC 5 cut(s) 1073, 3126, 3737, 3950, 3951
AcoI YGGCCR 1 cut(s) 4384
AcsI RAATTY 9 cut(s) 755, 834, 964, 1355, 2521, 2758, 3006, 3275, 3998
AcuI CTGAAG 9 cut(s) 516, 534, 1169, 1863, 3321, 3336, 4041, 4311, 4803
AcvI CACGTG 1 cut(s) 1587
AcyI GRCGYC 2 cut(s) 96, 1604
AdeI CACNNNGTG 1 cut(s) 1012
AfiI CCNNNNNNNGG 5 cut(s) 71, 944, 2404, 4128, 4499
AflII CTTAAG 2 cut(s) 443, 605
AflIII ACRYGT 4 cut(s) 1586, 2323, 2356, 3109
AhlI ACTAGT 1 cut(s) 2048
AleI CACNNNNGTG 2 cut(s) 1129, 1957
Alw21I GWGCWC 4 cut(s) 45, 288, 1204, 3376
Alw26I GTCTC 8 cut(s) 1253, 1550, 1595, 2110, 2268, 3448, 3541, 3972
Alw44I GTGCAC 2 cut(s) 1200, 3372
AlwI GGATC 5 cut(s) 1073, 3126, 3737, 3950, 3951
AlwNI CAGNNNCTG 2 cut(s) 4286, 4671
Ama87I CYCGRG 3 cut(s) 1063, 1562, 2166
Aor13HI TCCGGA 2 cut(s) 1081, 4262
AoxI GGCC 9 cut(s) 128, 955, 1399, 1412, 2188, 2746, 3779, 4384, 4480
ApaI GGGCCC 1 cut(s) 2192
ApaLI GTGCAC 2 cut(s) 1200, 3372
ApoI RAATTY 9 cut(s) 755, 834, 964, 1355, 2521, 2758, 3006, 3275, 3998
Asp700I GAANNNNTTC 3 cut(s) 261, 834, 4175
AspA2I CCTAGG 1 cut(s) 3671
AspLEI GCGC 2 cut(s) 98, 2952
AspS9I GGNCC 9 cut(s) 128, 473, 1399, 2114, 2188, 2189, 2633, 2816, 3779
AsuII TTCGAA 1 cut(s) 3343
AsuNHI GCTAGC 1 cut(s) 2966
AvaI CYCGRG 3 cut(s) 1063, 1562, 2166
AvaII GGWCC 4 cut(s) 473, 2114, 2633, 2816
AvrII CCTAGG 1 cut(s) 3671
BaeGI GKGCMC 4 cut(s) 1204, 2192, 3376, 4013
BaeI ACNNNNGTAYC 2 cut(s) 1133, 1166
BalI TGGCCA 1 cut(s) 4386
BanI GGYRCC 2 cut(s) 95, 4294
BanII GRGCYC 4 cut(s) 45, 288, 2192, 4832
BauI CACGAG 1 cut(s) 3774
BbrPI CACGTG 1 cut(s) 1587
BbsI GAAGAC 5 cut(s) 126, 540, 1566, 1890, 4401
Bbv12I GWGCWC 4 cut(s) 45, 288, 1204, 3376
BceAI ACGGC 3 cut(s) 109, 1648, 3723
BcgI CGANNNNNNTGC 4 cut(s) 2141, 2175, 3140, 3174
BciVI GTATCC 1 cut(s) 4176
BclI TGATCA 4 cut(s) 1102, 2293, 2314, 2539
BcoDI GTCTC 8 cut(s) 1253, 1550, 1595, 2110, 2268, 3448, 3541, 3972
BcuI ACTAGT 1 cut(s) 2048
BfmI CTRYAG 6 cut(s) 429, 653, 1317, 1441, 3102, 3611
BfoI RGCGCY 1 cut(s) 99
BfrI CTTAAG 2 cut(s) 443, 605
BfuAI ACCTGC 3 cut(s) 1448, 2557, 3133
BfuI GTATCC 1 cut(s) 4176
BglII AGATCT 1 cut(s) 2855
BlnI CCTAGG 1 cut(s) 3671
BmcAI AGTACT 2 cut(s) 1617, 1922
Bme18I GGWCC 4 cut(s) 473, 2114, 2633, 2816
BmeT110I CYCGRG 3 cut(s) 1063, 1562, 2166
BmgT120I GGNCC 9 cut(s) 128, 473, 1399, 2114, 2188, 2189, 2633, 2816, 3779
BmiI GGNNCC 8 cut(s) 63, 97, 507, 1627, 2115, 2189, 2190, 4296
BmtI GCTAGC 1 cut(s) 2970
BpiI GAAGAC 5 cut(s) 126, 540, 1566, 1890, 4401
BplI GAGNNNNNCTC 2 cut(s) 346, 378
BpmI CTGGAG 2 cut(s) 311, 1562
Bpu10I CCTNAGC 1 cut(s) 363
Bpu14I TTCGAA 1 cut(s) 3343
BpuEI CTTGAG 2 cut(s) 503, 767
Bsa29I ATCGAT 1 cut(s) 2985
BsaAI YACGTR 1 cut(s) 1587
BsaBI GATNNNNATC 2 cut(s) 3741, 4470
BsaHI GRCGYC 2 cut(s) 96, 1604
BsaI GGTCTC 2 cut(s) 1253, 2268
BsaWI WCCGGW 3 cut(s) 1081, 1879, 4262
Bsc4I CCNNNNNNNGG 5 cut(s) 71, 944, 2404, 4128, 4499
Bse1I ACTGG 3 cut(s) 415, 3397, 4387
Bse3DI GCAATG 8 cut(s) 489, 1047, 1599, 2619, 3079, 3726, 3987, 4567
Bse8I GATNNNNATC 2 cut(s) 3741, 4470
BseAI TCCGGA 2 cut(s) 1081, 4262
BseCI ATCGAT 1 cut(s) 2985
BseJI GATNNNNATC 2 cut(s) 3741, 4470
BseLI CCNNNNNNNGG 5 cut(s) 71, 944, 2404, 4128, 4499
BseMI GCAATG 8 cut(s) 489, 1047, 1599, 2619, 3079, 3726, 3987, 4567
BseMII CTCAG 4 cut(s) 365, 377, 3441, 3609
BseNI ACTGG 3 cut(s) 415, 3397, 4387
BseRI GAGGAG 7 cut(s) 233, 407, 410, 449, 515, 1862, 2453
BseSI GKGCMC 4 cut(s) 1204, 2192, 3376, 4013
BseYI CCCAGC 1 cut(s) 4671
BsgI GTGCAG 4 cut(s) 1034, 1935, 2145, 2535
Bsh1236I CGCG 1 cut(s) 3558
Bsh1285I CGRYCG 3 cut(s) 69, 88, 4462
BshFI GGCC 9 cut(s) 130, 957, 1401, 1414, 2190, 2748, 3781, 4386, 4482
BshNI GGYRCC 2 cut(s) 95, 4294
BshVI ATCGAT 1 cut(s) 2985
BsiEI CGRYCG 3 cut(s) 69, 88, 4462
BsiHKAI GWGCWC 4 cut(s) 45, 288, 1204, 3376
BsiHKCI CYCGRG 3 cut(s) 1063, 1562, 2166
BsiSI CCGG 3 cut(s) 1082, 1880, 4263
BslFI GGGAC 7 cut(s) 89, 339, 809, 992, 2127, 3446, 4120
BslI CCNNNNNNNGG 5 cut(s) 71, 944, 2404, 4128, 4499
BsmAI GTCTC 8 cut(s) 1253, 1550, 1595, 2110, 2268, 3448, 3541, 3972
BsmBI CGTCTC 1 cut(s) 1595
BsmFI GGGAC 7 cut(s) 89, 339, 809, 992, 2127, 3446, 4120
BsmI GAATGC 2 cut(s) 1064, 4768
BsnI GGCC 9 cut(s) 130, 957, 1401, 1414, 2190, 2748, 3781, 4386, 4482
Bso31I GGTCTC 2 cut(s) 1253, 2268
BsoBI CYCGRG 3 cut(s) 1063, 1562, 2166
Bsp119I TTCGAA 1 cut(s) 3343
Bsp120I GGGCCC 1 cut(s) 2188
Bsp1286I GDGCHC 7 cut(s) 45, 288, 1204, 2192, 3376, 4013, 4832
Bsp13I TCCGGA 2 cut(s) 1081, 4262
Bsp1407I TGTACA 1 cut(s) 3328
BspANI GGCC 9 cut(s) 130, 957, 1401, 1414, 2190, 2748, 3781, 4386, 4482
BspCNI CTCAG 4 cut(s) 364, 376, 3442, 3610
BspDI ATCGAT 1 cut(s) 2985
BspEI TCCGGA 2 cut(s) 1081, 4262
BspFNI CGCG 1 cut(s) 3558
BspHI TCATGA 1 cut(s) 2337
BspLI GGNNCC 8 cut(s) 63, 97, 507, 1627, 2115, 2189, 2190, 4296
BspMAI CTGCAG 4 cut(s) 433, 657, 1321, 1445
BspMI ACCTGC 3 cut(s) 1448, 2557, 3133
BspOI GCTAGC 1 cut(s) 2970
BspPI GGATC 5 cut(s) 1073, 3126, 3737, 3950, 3951
BspT104I TTCGAA 1 cut(s) 3343
BspT107I GGYRCC 2 cut(s) 95, 4294
BspTI CTTAAG 2 cut(s) 443, 605
BspTNI GGTCTC 2 cut(s) 1253, 2268
BsrDI GCAATG 8 cut(s) 489, 1047, 1599, 2619, 3079, 3726, 3987, 4567
BsrGI TGTACA 1 cut(s) 3328
BsrI ACTGG 3 cut(s) 415, 3397, 4387
BssNI GRCGYC 2 cut(s) 96, 1604
BssSI CACGAG 1 cut(s) 3774
BssT1I CCWWGG 1 cut(s) 3671
Bst2BI CACGAG 1 cut(s) 3774
Bst4CI ACNGT 7 cut(s) 82, 89, 212, 934, 1497, 1959, 3340
Bst6I CTCTTC 6 cut(s) 171, 192, 492, 558, 1839, 2635
BstACI GRCGYC 2 cut(s) 96, 1604
BstAFI CTTAAG 2 cut(s) 443, 605
BstAPI GCANNNNNTGC 4 cut(s) 1012, 1751, 2618, 4748
BstAUI TGTACA 1 cut(s) 3328
BstBAI YACGTR 1 cut(s) 1587
BstBI TTCGAA 1 cut(s) 3343
BstDEI CTNAG 7 cut(s) 351, 363, 1416, 1928, 3260, 3450, 3618
BstENI CCTNNNNNAGG 1 cut(s) 2402
BstFNI CGCG 1 cut(s) 3558
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 2 cut(s) 98, 2952
BstMAI GTCTC 8 cut(s) 1253, 1550, 1595, 2110, 2268, 3448, 3541, 3972
BstMCI CGRYCG 3 cut(s) 69, 88, 4462
BstNSI RCATGY 3 cut(s) 362, 2360, 3113
BstSFI CTRYAG 6 cut(s) 429, 653, 1317, 1441, 3102, 3611
BstSLI GKGCMC 4 cut(s) 1204, 2192, 3376, 4013
BstUI CGCG 1 cut(s) 3558
BstV2I GAAGAC 5 cut(s) 126, 540, 1566, 1890, 4401
BstX2I RGATCY 3 cut(s) 1078, 2855, 3742
BstXI CCANNNNNNTGG 2 cut(s) 1636, 4632
BstYI RGATCY 3 cut(s) 1078, 2855, 3742
Bsu15I ATCGAT 1 cut(s) 2985
BsuI GTATCC 1 cut(s) 4176
BsuRI GGCC 9 cut(s) 130, 957, 1401, 1414, 2190, 2748, 3781, 4386, 4482
BsuTUI ATCGAT 1 cut(s) 2985
BtgZI GCGATG 1 cut(s) 4824
BtsI GCAGTG 6 cut(s) 862, 1202, 2959, 4351, 4660, 4672
BtsIMutI CAGTG 7 cut(s) 217, 862, 1202, 2959, 4351, 4660, 4672
BveI ACCTGC 3 cut(s) 1448, 2557, 3133
CaiI CAGNNNCTG 2 cut(s) 4286, 4671
CciI TCATGA 1 cut(s) 2337
CfoI GCGC 2 cut(s) 98, 2952
Cfr13I GGNCC 9 cut(s) 128, 473, 1399, 2114, 2188, 2189, 2633, 2816, 3779
ClaI ATCGAT 1 cut(s) 2985
CseI GACGC 2 cut(s) 1254, 1612
CsiI ACCWGGT 1 cut(s) 2739
CspCI CAANNNNNGTGG 2 cut(s) 4354, 4389
DdeI CTNAG 7 cut(s) 351, 363, 1416, 1928, 3260, 3450, 3618
DinI GGCGCC 1 cut(s) 97
DraI TTTAAA 1 cut(s) 1275
DraIII CACNNNGTG 1 cut(s) 1012
EaeI YGGCCR 1 cut(s) 4384
Eam1104I CTCTTC 6 cut(s) 171, 192, 492, 558, 1839, 2635
EarI CTCTTC 6 cut(s) 171, 192, 492, 558, 1839, 2635
EciI GGCGGA 2 cut(s) 221, 393
Ecl136II GAGCTC 2 cut(s) 43, 286
Eco130I CCWWGG 1 cut(s) 3671
Eco147I AGGCCT 3 cut(s) 957, 1414, 2748
Eco24I GRGCYC 4 cut(s) 45, 288, 2192, 4832
Eco31I GGTCTC 2 cut(s) 1253, 2268
Eco32I GATATC 2 cut(s) 3868, 4089
Eco47I GGWCC 4 cut(s) 473, 2114, 2633, 2816
Eco53kI GAGCTC 2 cut(s) 43, 286
Eco57I CTGAAG 9 cut(s) 516, 534, 1169, 1863, 3321, 3336, 4041, 4311, 4803
Eco72I CACGTG 1 cut(s) 1587
Eco88I CYCGRG 3 cut(s) 1063, 1562, 2166
EcoICRI GAGCTC 2 cut(s) 43, 286
EcoNI CCTNNNNNAGG 1 cut(s) 2402
EcoO109I RGGNCCY 3 cut(s) 473, 2188, 2189
EcoRI GAATTC 3 cut(s) 834, 3006, 3275
EcoRV GATATC 2 cut(s) 3868, 4089
EcoT14I CCWWGG 1 cut(s) 3671
EcoT38I GRGCYC 4 cut(s) 45, 288, 2192, 4832
EgeI GGCGCC 1 cut(s) 97
EheI GGCGCC 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 3671
Esp3I CGTCTC 1 cut(s) 1595
FalI AAGNNNNNCTT 4 cut(s) 1439, 1471, 3294, 3326
FaqI GGGAC 7 cut(s) 89, 339, 809, 992, 2127, 3446, 4120
FauI CCCGC 1 cut(s) 4312
FauNDI CATATG 3 cut(s) 1635, 3157, 4495
FbaI TGATCA 4 cut(s) 1102, 2293, 2314, 2539
FblI GTMKAC 3 cut(s) 84, 162, 3610
FriOI GRGCYC 4 cut(s) 45, 288, 2192, 4832
GlaI GCGC 2 cut(s) 97, 2951
GsaI CCCAGC 1 cut(s) 4675
GsuI CTGGAG 2 cut(s) 311, 1562
HaeII RGCGCY 1 cut(s) 99
HaeIII GGCC 9 cut(s) 130, 957, 1401, 1414, 2190, 2748, 3781, 4386, 4482
HapII CCGG 3 cut(s) 1082, 1880, 4263
HgaI GACGC 2 cut(s) 1254, 1612
HhaI GCGC 2 cut(s) 98, 2952
Hin1I GRCGYC 2 cut(s) 96, 1604
Hin6I GCGC 2 cut(s) 96, 2950
HinP1I GCGC 2 cut(s) 96, 2950
HincII GTYRAC 2 cut(s) 85, 2046
HindII GTYRAC 2 cut(s) 85, 2046
HindIII AAGCTT 5 cut(s) 440, 602, 743, 1853, 4617
HpaII CCGG 3 cut(s) 1082, 1880, 4263
Hpy166II GTNNAC 9 cut(s) 85, 163, 1202, 1348, 1716, 2046, 2633, 3374, 3611
Hpy8I GTNNAC 9 cut(s) 85, 163, 1202, 1348, 1716, 2046, 2633, 3374, 3611
Hpy99I CGWCG 8 cut(s) 26, 29, 50, 74, 104, 1248, 3340, 4008
HpyCH4III ACNGT 7 cut(s) 82, 89, 212, 934, 1497, 1959, 3340
HpyCH4IV ACGT 5 cut(s) 703, 1586, 2286, 2323, 3951
HpyF3I CTNAG 7 cut(s) 351, 363, 1416, 1928, 3260, 3450, 3618
HpySE526I ACGT 5 cut(s) 703, 1586, 2286, 2323, 3951
Hsp92I GRCGYC 2 cut(s) 96, 1604
HspAI GCGC 2 cut(s) 96, 2950
KasI GGCGCC 1 cut(s) 95
Kpn2I TCCGGA 2 cut(s) 1081, 4262
Ksp22I TGATCA 4 cut(s) 1102, 2293, 2314, 2539
LmnI GCTCC 6 cut(s) 291, 1625, 1686, 2282, 2372, 3646
MabI ACCWGGT 1 cut(s) 2739
MaeII ACGT 5 cut(s) 703, 1586, 2286, 2323, 3951
MfeI CAATTG 1 cut(s) 1746
MflI RGATCY 3 cut(s) 1078, 2855, 3742
MhlI GDGCHC 7 cut(s) 45, 288, 1204, 2192, 3376, 4013, 4832
MlsI TGGCCA 1 cut(s) 4386
MluNI TGGCCA 1 cut(s) 4386
Mly113I GGCGCC 1 cut(s) 96
MlyI GAGTC 4 cut(s) 1552, 2178, 4268, 4781
MmeI TCCRAC 7 cut(s) 45, 1002, 2437, 2911, 2973, 4027, 4358
Mox20I TGGCCA 1 cut(s) 4386
MroI TCCGGA 2 cut(s) 1081, 4262
MroXI GAANNNNTTC 3 cut(s) 261, 834, 4175
MscI TGGCCA 1 cut(s) 4386
MslI CAYNNNNRTG 6 cut(s) 1094, 1129, 1957, 3834, 4152, 4476
Msp20I TGGCCA 1 cut(s) 4386
MspA1I CMGCKG 1 cut(s) 512
MspCI CTTAAG 2 cut(s) 443, 605
MspI CCGG 3 cut(s) 1082, 1880, 4263
MunI CAATTG 1 cut(s) 1746
Mva1269I GAATGC 2 cut(s) 1064, 4768
MvnI CGCG 1 cut(s) 3558
NarI GGCGCC 1 cut(s) 96
NdeI CATATG 3 cut(s) 1635, 3157, 4495
NheI GCTAGC 1 cut(s) 2966
NlaIV GGNNCC 8 cut(s) 63, 97, 507, 1627, 2115, 2189, 2190, 4296
NmuCI GTSAC 5 cut(s) 89, 1305, 1425, 1907, 3467
NspI RCATGY 3 cut(s) 362, 2360, 3113
NspV TTCGAA 1 cut(s) 3343
OliI CACNNNNGTG 2 cut(s) 1129, 1957
PaeI GCATGC 1 cut(s) 362
PaeR7I CTCGAG 1 cut(s) 2166
PagI TCATGA 1 cut(s) 2337
PasI CCCWGGG 1 cut(s) 2192
PceI AGGCCT 3 cut(s) 957, 1414, 2748
PciI ACATGT 2 cut(s) 2356, 3109
PcsI WCGNNNNNNNCGW 5 cut(s) 45, 51, 99, 156, 4000
PctI GAATGC 2 cut(s) 1064, 4768
PdmI GAANNNNTTC 3 cut(s) 261, 834, 4175
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 288
Ple19I CGATCG 1 cut(s) 69
PleI GAGTC 4 cut(s) 1552, 2177, 4267, 4781
PluTI GGCGCC 1 cut(s) 99
PmaCI CACGTG 1 cut(s) 1587
PmlI CACGTG 1 cut(s) 1587
PpsI GAGTC 4 cut(s) 1552, 2177, 4267, 4781
Ppu21I YACGTR 1 cut(s) 1587
PpuMI RGGWCCY 1 cut(s) 473
PscI ACATGT 2 cut(s) 2356, 3109
PsiI TTATAA 1 cut(s) 971
Psp124BI GAGCTC 2 cut(s) 45, 288
Psp1406I AACGTT 1 cut(s) 2286
Psp5II RGGWCCY 1 cut(s) 473
PspCI CACGTG 1 cut(s) 1587
PspFI CCCAGC 1 cut(s) 4671
PspN4I GGNNCC 8 cut(s) 63, 97, 507, 1627, 2115, 2189, 2190, 4296
PspOMI GGGCCC 1 cut(s) 2188
PspPI GGNCC 9 cut(s) 128, 473, 1399, 2114, 2188, 2189, 2633, 2816, 3779
PspPPI RGGWCCY 1 cut(s) 473
PspXI VCTCGAGB 1 cut(s) 2166
PsrI GAACNNNNNNTAC 2 cut(s) 3503, 3535
PstI CTGCAG 4 cut(s) 433, 657, 1321, 1445
PstNI CAGNNNCTG 2 cut(s) 4286, 4671
PsuI RGATCY 3 cut(s) 1078, 2855, 3742
PsyI GACNNNGTC 1 cut(s) 80
PvuI CGATCG 1 cut(s) 69
PvuII CAGCTG 1 cut(s) 512
RseI CAYNNNNRTG 6 cut(s) 1094, 1129, 1957, 3834, 4152, 4476
SacI GAGCTC 2 cut(s) 45, 288
SalI GTCGAC 1 cut(s) 83
Sau96I GGNCC 9 cut(s) 128, 473, 1399, 2114, 2188, 2189, 2633, 2816, 3779
ScaI AGTACT 2 cut(s) 1617, 1922
SchI GAGTC 4 cut(s) 1552, 2178, 4268, 4781
SduI GDGCHC 7 cut(s) 45, 288, 1204, 2192, 3376, 4013, 4832
SexAI ACCWGGT 1 cut(s) 2739
SfcI CTRYAG 6 cut(s) 429, 653, 1317, 1441, 3102, 3611
SfoI GGCGCC 1 cut(s) 97
Sfr274I CTCGAG 1 cut(s) 2166
SfuI TTCGAA 1 cut(s) 3343
SinI GGWCC 4 cut(s) 473, 2114, 2633, 2816
SlaI CTCGAG 1 cut(s) 2166
SmiMI CAYNNNNRTG 6 cut(s) 1094, 1129, 1957, 3834, 4152, 4476
SmlI CTYRAG 5 cut(s) 443, 482, 605, 746, 2166
SmoI CTYRAG 5 cut(s) 443, 482, 605, 746, 2166
SpeI ACTAGT 1 cut(s) 2048
SphI GCATGC 1 cut(s) 362
SseBI AGGCCT 3 cut(s) 957, 1414, 2748
SspDI GGCGCC 1 cut(s) 95
SspI AATATT 2 cut(s) 2868, 3703
SstI GAGCTC 2 cut(s) 45, 288
StuI AGGCCT 3 cut(s) 957, 1414, 2748
StyI CCWWGG 1 cut(s) 3671
TaaI ACNGT 7 cut(s) 82, 89, 212, 934, 1497, 1959, 3340
TaiI ACGT 5 cut(s) 706, 1589, 2289, 2326, 3954
TatI WGTACW 5 cut(s) 1615, 1920, 2144, 3328, 4133
TauI GCSGC 2 cut(s) 340, 4665
TscAI CASTG 7 cut(s) 217, 862, 1209, 2959, 4351, 4660, 4672
TseFI GTSAC 5 cut(s) 89, 1305, 1425, 1907, 3467
Tsp45I GTSAC 5 cut(s) 89, 1305, 1425, 1907, 3467
TspGWI ACGGA 8 cut(s) 1661, 1749, 1991, 2090, 2140, 2317, 3872, 4144
TspRI CASTG 7 cut(s) 217, 862, 1209, 2959, 4351, 4660, 4672
Tth111I GACNNNGTC 1 cut(s) 80
Vha464I CTTAAG 2 cut(s) 443, 605
VneI GTGCAC 2 cut(s) 1200, 3372
VpaK11BI GGWCC 4 cut(s) 473, 2114, 2633, 2816
XagI CCTNNNNNAGG 1 cut(s) 2402
XapI RAATTY 9 cut(s) 755, 834, 964, 1355, 2521, 2758, 3006, 3275, 3998
XceI RCATGY 3 cut(s) 362, 2360, 3113
XcmI CCANNNNNNNNNTGG 1 cut(s) 4154
XhoI CTCGAG 1 cut(s) 2166
XmaJI CCTAGG 1 cut(s) 3671
XmiI GTMKAC 3 cut(s) 84, 162, 3610
XmnI GAANNNNTTC 3 cut(s) 261, 834, 4175
ZrmI AGTACT 2 cut(s) 1617, 1922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.