RLG00000022666

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009494
Physical Location & Seq
Reverse (-)
7126720 .. 7135499
8780 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022666

Sequence Viewer

Length: 4920 bp
ATGGGCAAAGCTGTCATCTCCGACGACGAGGATGAGGTCGAGCTCGACGAAGAAGATAGGGAACCCCTCGACGGGGACACAGTCGACCAGGACGGCGGCGGCGACGAAGATGAGGACGAAGAAGACGAAGGGCCAGATGAATATGAGAAGGATGATTTTATCGTAGATGATGTTGATGAAGAGGATGAGCAGGAGGAAGAAGAGCGGGCCGACAGTGATGAGGAGAGGCAGAAGAAGAAGAAAAGAAAGAAAAAAGAGGAGTATGTTCTTGATGAAGATGACTACGAGCTCCTGGAGGACAATAATGTGATTGCCCCGCGCAGAAAGGGGAAATTTAAGCGGCTGAAAAAAGCTCAGAGGCATGCTCAGGGGGACCTTGGTGGATTATCTGATGAGGAGGAGTTTCTTGGTTCTGGTAAAGGGGGAAGAACTGCAGAGGAGAAGCTTAAGTTCTCATTATTTGGTGGTGATGATGAAGGTCCTCCACTTGAGGACATTGCTGAAGAGGAGGAACCAGCCGAAGCGGAAGATGACGGAGAAGATGAAATGGCAGACTTTATAGTCGACGAAGAATTTGATGAGGCTGGTGTTCCTGTCAGACAGAAGAAGCTTAAGAAAAAGAAATCCAGGCAGGCACCAGGAGTATCTTCATCTGCTCTGCAGGAAGCCCATGACATATTCGGTGATGTTGATGAATTACTGCAGCTGCGTAAGCAAGGTTTGGATTCTAGTGAATGGAAGGAAAGGAGGCTTGAAGATGAATTTGAACCAATTGTTCTTTCGGAAAAGTATATGACAGAGAAGGATGACCAGATTCGGGAGATTGATGTCCCAGAAAGAATTCAGGTGTATGAGGAAAGTACTGGTTTTCCTCCACTTGATGAAAAGAGCATAGATGATGAAAGTACCTGGATATTCAATCAACTTGCAAGTGGTACAGTTCCTTTTTTTGGCAAGACAGGCCTGGGGAATTCCATAAGCAGGGACGACATCATTGGATTCTTGAACTTGCACCATGTGCAGAAATTGGATGTTCCTTTTATTGCGATGTATCGGAAAGAGGAGTGCCTTAGTATATTGAAAGATCCTGAACACATGGACATGGATGATCAAAATGAGAAAGCATCCACACTAAAGTGGCACAAGGTACTTTGGTCCATTCAGGACTTGCATAGAAAATGGTTGCTGCTTCAGAAGAGGAAAAGTGCTCTGCAGTCTTATTACGAAAAGCGGTTTGATGAAGAATCTCGACGCATATATGATGAGACCAGACTAACTTTAAATCAGCAACTTTTTGAATCAATTATGAAGTCACTAAAGGCTGCAGAATCTGAGAGAGAAGTTGATGATGTGGACACCAAGTTCAACTTGCATTTCCCTGCTGGTGAGATCGGTGTGGATGAGGGCCAATATAAGAGGCCTAAGAGGAAGTCACTTTATAGCATCTGCAGTAAGGCTGGCTTGTGGGAGGTTGCCAGCAAGTTTGGTTACACCTCTGAACAGTTTGGGTTGCAATTATCACTCGAGAAAATGAGAATGGATGAGCTGGAGGATGCGAAAGAGACGCCAGAGGAGTTGGCTTCAAACTTCACATGTGCAATGTTTGAGACTCCTCAGGAAGTGCTCAAAGGAGCCAGGCATATGGCTGCTGTTGAGATAAGCTGTGAGCCATGTGTCAGGAAGTATGTTCGGAGCAACTACTTGGATATGGTTGAGTTGTCCACGAGCCCAACACCAGATGGAAATGCTGCAATTGAAGCTTTTCATCAGTTTGCAGGGGTGAAGTGGTTGCAAAGAAAGCCATTAAATAGATTTGGGGATGCACAATGGCTGCTTATCCAGAAGGCTGAAGAGGAGAAGCTTCTTCAAGTTACTATTAAGCTACCGGAAGACAGACTAAACAAGTTGATGAGCGACTTTAATGAGTACTATCTTAGTGATGGTGTCAGTAAATCTGCACAACTGTGGAATGAGCAGCGGAAGTTGATATTGCAGGATGCTCTTTTCAATTTTCTTCTACCTTCAATGGAGAAAGAAGCAAGATCCTTGTTGACTAGTAGGGCAAAAAACTGGTTATTGATGGAATACGGAAAGGTTTTGTGGAATAAAGTCTCTGTGGGACCGTATCAACGGAAAGAGAATGACATTAGTTCAGACGATGAAGCTGCACCCAGGGTCATGGCTTGCTGTTGGGGACCTGGGAAGCCAGCAACCACTTTTGTTATGTTAGATTCATCGGGGGAGGTGCTAGATGTGCTTTACACAGGATCTCTCACCTTACGCTCCCAGAATGTTAATGATCAGCAACGTAAGAAAAATGACCAAGAACGTGTTTTGAAGTTCATGACTGATCACCAACCACATGTGGCTGTTCTAGGAGCTGTTAATTTGTCTTGTGTTCGCCTGAAAGAAGATATTTACGAGATAATCTTTAAGATGGTGGAGGAGAATCCTAGAGATGTTGGACATGATATGGATGGGCTAAGCATTGTCTATGGAGATGAATCTTTGGCTCGCCTGTATGAAAACTCTCGCATTTCATCTGATCAGCTACCTGCACAATCAGGCATTGTGAAGCGTGCTGTTGCTCTGGGGCGTTACCTTCAAAATCCATTGGCAATGGTCGCCACTCTTTGTGGGCCAGGAAGAGAAATCTTATCTTGGAAACTCAATCCTTTGGAGAACTTTCTCACCCCAGATGAGAAGTATGCAATGATTGAACAAGTAATGGTAGATGTTACGAACCAAGTAGGCCTCGACATAAATTTGGCTATAAGCCACGAATGGTTATTTGCACCTTTGCAATTCATTTCTGGGCTCGGACCTAGAAAAGCAGCATTTTTGCAGCGATCGTTGGTAAGATCTGGTGCAATATTTACTCGGAAGGACTTTTTGACAGCACATGGGTTAAGTAAAAAGGTGTTTGTTAATGCAGTTGGTTTCTTGCGTGTTCGGCGCAGTGGGTTGGCTGCTAGCAGCAGCCAATTTATTGATCTGTTGGATGATACAAGAATTCATCCAGAATCCTATATTCTTGCACAAGAATTGGCCAAAGATGTTTTTGAAGTTGATGGTGGCAATGATGATGAGGATGCTATGGAGATGGCTATAGAACATGTTAGGGATCGGCCTGCTTATCTAAAAAATCTTGATGTTGAAGCGTATGCTAAAAGCAAAGAGCGTGAGAATAAGATACAAACATTTTGTGACATCAGGAGAGAATTAATACAGGGTTTTCAGGATTGGCGTAAGAAATATGAAGAACTAAGTCAAGATGAAGAATTCTATATGATTTCAGGTGAAACTGAAGATACCCTTGCTGAAGGAAGAATTGTGCTGGCCACAGTTCGCAGGGTACAAGCTCAAAGGGCAATTTGTGCACTTGAATCTGGACTGACTGGTATGCTTATGAAAGAAGACTATTCAGATGATTCGAGGGATATTTCTGACTTATCTGAGAGACTGAATGAGGGTGACATTCTCACATGCAAGATCAAATCTATTCAAAAGAATAGGTATCAGGTGTTCCTAGTTTGTAGAGAGAGTGAAATGAGAAATAACCGAAATCAAAATATTCAGCATCTCGATTCCTATTACCATGAAGACAGGAGAAGTCTACAGACAGAGCAAGAAAAAGCTCGTAAGGAGAAAGAGCTTGCGAAAAAACATTTCAAGCCAAGGATGATTGTTCATCCTCGCTTCCAGAATATTACTGCTGACGAAGCAATGAAGTTCTTATCTGATAAAGATCCTGGGGAAAGTATTATTCGTCCTAGTTCCCGTGGGCCTTCGTATTTGACCTTAACTCTGAAAGTTTACGATGGAGTTTATGCTCACAAAGACGTAGTTGAAGGTGGAAAGGAACACAAGGATATCACCAGCTTACTCCGTATTGGAAAGACTCTGAAAATTGGAGAGGACACTTTTGAAGATTTAGATGAGGTAATGGATCGTTATGTTGATCCCCTGGTGTCTCACTTGAAGGCAATGCTAAATTATCGCAAATTCCGACGGGGCACAAAAGCAGAGGTTGATGAACTGTTGAAGATTGAAAAATTGGAGTTTCCTATGAGGATTGTTTATTGTTTCGGGATATCACATGAACATCCAGGGACTTTCATACTGACCTATATACGGAGTACAAATCCACACCATGAGTATGTTGGGCTGTATCCTAAGGGGTTCAAGTTCAGAAAAAGGATGTTTGAGAGCATTGATCGGCTTGTAGCACATTTTCAGAAGTATATTGATGAGCCTCAACATGAGTCTGGACCATCAATTAGATCAGTTGCTGCTATGGTGCCCATGCGAAGCCCTGCAACTGGGGGTTCTTCGGGTGCATCCGCAGGATGGGGTGGTTCAAACAATGAGGGTGGTTGGAGAGGCCAGTCATTTGACAGGGATAGGTCTTCTACACCGAGTTCACGAACAGGCAGAAATGATTACAGAAATGGTGGCGGTCGAGATGGACATCCAAGTGGCCTACCTAGACCATATGGGGGGCGCGGTCGTGGTCGAGGAACATATAACGATAGCCGAGGCAATAATGAGAGACATGATTCAGGTTTTGATGGTCCTACATGGGGTTCAGATACCAAGGATGGCAATGATGGCTTGGGCAACTTTCCAGGTGCAAAGGTTCAAAACTCACCTGGCAGGGAAGCTTTCCCAGGTGGCTGGGGTACAGGTGGAAGTGGTAGTGGAGGAGGCAGCAGCTGGGCTGCTGGTGGTGGAAGTGGCTGGGGTCAAGAAAGTGGTGGTGGTTCAGGAGGATGGGGTGGGGCTGCTGCAAATGATGCTGCTGCTGATAATGAGCCTTCAGGATGGGGCTCTGACTCGAAAAAGAAGGGTTCAGGTGATGGCACTTCAGGATGGGGTTCTCAGAGTGGAGGTGGCGGCGGCGGCGGTGGTGGTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

1640

Amino Acids

184.83

Weight (kDa)

5.0

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT6_acidic PF14632 35 - 120 1.8e-16 Acidic N-terminal SPT6
HTH_44 PF14641 293 - 410 2.6e-06 Helix-turn-helix DNA-binding domain of SPT6
Tex_central_region PF22706 519 - 688 1.4e-06 Tex central region-like
YqgF PF14639 723 - 879 4.2e-17 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 883 - 984 1.1e-20 Helix-hairpin-helix motif
Spt6_S1 PF21710 1095 - 1183 1.2e-08 Spt6, S1/OB domain
SH2_2 PF14633 1199 - 1414 6.8e-69 SH2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 560
Acc36I ACCTGC 1 cut(s) 2563
AccB1I GGYRCC 2 cut(s) 634, 4300
AccBSI CCGCTC 1 cut(s) 205
AccI GTMKAC 3 cut(s) 84, 564, 3616
AccII CGCG 2 cut(s) 319, 4506
AclWI GGATC 7 cut(s) 1079, 2037, 2275, 3132, 3743, 3956, 3957
AcoI YGGCCR 2 cut(s) 3048, 3339
AcsI RAATTY 9 cut(s) 332, 572, 761, 840, 970, 2764, 3012, 3281, 4004
AcuI CTGAAG 7 cut(s) 522, 1175, 1869, 3327, 3342, 4803, 4851
AcyI GRCGYC 1 cut(s) 1565
AdeI CACNNNGTG 1 cut(s) 1018
AfaI GTAC 8 cut(s) 862, 907, 937, 1149, 1928, 3357, 4141, 4684
AflII CTTAAG 2 cut(s) 446, 611
AflIII ACRYGT 4 cut(s) 1592, 2329, 2362, 3115
AhlI ACTAGT 1 cut(s) 2054
AjuI GAANNNNNNNTTGG 2 cut(s) 763, 795
AleI CACNNNNGTG 2 cut(s) 1135, 1963
AloI GAACNNNNNNTCC 2 cut(s) 1346, 1378
Alw21I GWGCWC 5 cut(s) 45, 291, 1210, 1626, 3382
Alw26I GTCTC 7 cut(s) 1259, 1556, 1601, 2116, 3454, 3978, 4546
Alw44I GTGCAC 1 cut(s) 3378
AlwI GGATC 7 cut(s) 1079, 2037, 2275, 3132, 3743, 3956, 3957
AlwNI CAGNNNCTG 3 cut(s) 1331, 4292, 4716
Ama87I CYCGRG 1 cut(s) 1523
ApaLI GTGCAC 1 cut(s) 3378
ApoI RAATTY 9 cut(s) 332, 572, 761, 840, 970, 2764, 3012, 3281, 4004
ArsI GACNNNNNNTTYG 2 cut(s) 557, 589
AseI ATTAAT 1 cut(s) 3224
Asp700I GAANNNNTTC 2 cut(s) 840, 1761
AspLEI GCGC 3 cut(s) 321, 2958, 4506
AsuNHI GCTAGC 1 cut(s) 2972
AvaI CYCGRG 1 cut(s) 1523
AvaII GGWCC 8 cut(s) 373, 479, 1155, 2120, 2195, 2822, 4271, 4574
AxyI CCTNAGG 2 cut(s) 1614, 4176
BaeGI GKGCMC 3 cut(s) 3382, 4019, 4305
BaeI ACNNNNGTAYC 2 cut(s) 1139, 1172
BalI TGGCCA 2 cut(s) 3050, 3341
BanI GGYRCC 2 cut(s) 634, 4300
BanII GRGCYC 5 cut(s) 45, 291, 1730, 2820, 4832
BauI CACGAG 1 cut(s) 1723
BbsI GAAGAC 5 cut(s) 129, 1896, 3423, 3609, 4401
Bbv12I GWGCWC 5 cut(s) 45, 291, 1210, 1626, 3382
BceAI ACGGC 1 cut(s) 109
BcgI CGANNNNNNTGC 2 cut(s) 2147, 2181
BciVI GTATCC 1 cut(s) 4182
BclI TGATCA 4 cut(s) 1108, 2299, 2350, 2545
BcoDI GTCTC 7 cut(s) 1259, 1556, 1601, 2116, 3454, 3978, 4546
BcuI ACTAGT 1 cut(s) 2054
BfmI CTRYAG 8 cut(s) 432, 659, 701, 1211, 1323, 1447, 3108, 3617
BfrI CTTAAG 2 cut(s) 446, 611
BfuAI ACCTGC 1 cut(s) 2563
BfuI GTATCC 1 cut(s) 4182
BglII AGATCT 1 cut(s) 2861
BlpI GCTNAGC 1 cut(s) 2483
BmcAI AGTACT 2 cut(s) 862, 1928
Bme18I GGWCC 8 cut(s) 373, 479, 1155, 2120, 2195, 2822, 4271, 4574
BmeT110I CYCGRG 1 cut(s) 1523
BmiI GGNNCC 8 cut(s) 63, 374, 513, 636, 1633, 2121, 2196, 4302
BmrI ACTGGG 1 cut(s) 4332
BmsI GCATC 9 cut(s) 1133, 1452, 1543, 1810, 1987, 3082, 3589, 4349, 4786
BmtI GCTAGC 1 cut(s) 2976
BmuI ACTGGG 1 cut(s) 4332
BpiI GAAGAC 5 cut(s) 129, 1896, 3423, 3609, 4401
BplI GAGNNNNNCTC 2 cut(s) 349, 381
BpmI CTGGAG 2 cut(s) 314, 1568
Bpu10I CCTNAGC 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 2483
BpuEI CTTGAG 1 cut(s) 509
BsaBI GATNNNNATC 2 cut(s) 3747, 4470
BsaHI GRCGYC 1 cut(s) 1565
BsaI GGTCTC 1 cut(s) 1259
BsaWI WCCGGW 1 cut(s) 1885
Bse1I ACTGG 5 cut(s) 868, 2075, 3403, 4327, 4387
Bse21I CCTNAGG 2 cut(s) 1614, 4176
Bse3DI GCAATG 8 cut(s) 495, 1605, 2625, 2718, 3085, 3732, 3993, 4612
Bse8I GATNNNNATC 2 cut(s) 3747, 4470
BseJI GATNNNNATC 2 cut(s) 3747, 4470
BseMI GCAATG 8 cut(s) 495, 1605, 2625, 2718, 3085, 3732, 3993, 4612
BseMII CTCAG 6 cut(s) 368, 380, 1323, 1628, 3447, 4895
BseNI ACTGG 5 cut(s) 868, 2075, 3403, 4327, 4387
BseSI GKGCMC 3 cut(s) 3382, 4019, 4305
BseYI CCCAGC 3 cut(s) 4677, 4716, 4740
BsgI GTGCAG 4 cut(s) 1040, 1941, 2151, 2541
Bsh1236I CGCG 2 cut(s) 319, 4506
Bsh1285I CGRYCG 3 cut(s) 2852, 4462, 4510
BshNI GGYRCC 2 cut(s) 634, 4300
BsiEI CGRYCG 3 cut(s) 2852, 4462, 4510
BsiHKAI GWGCWC 5 cut(s) 45, 291, 1210, 1626, 3382
BsiHKCI CYCGRG 1 cut(s) 1523
BsiSI CCGG 1 cut(s) 1886
BslFI GGGAC 7 cut(s) 89, 386, 815, 998, 2133, 2208, 4126
BsmAI GTCTC 7 cut(s) 1259, 1556, 1601, 2116, 3454, 3978, 4546
BsmBI CGTCTC 1 cut(s) 1556
BsmFI GGGAC 7 cut(s) 89, 386, 815, 998, 2133, 2208, 4126
Bso31I GGTCTC 1 cut(s) 1259
BsoBI CYCGRG 1 cut(s) 1523
Bsp1720I GCTNAGC 1 cut(s) 2483
BspCNI CTCAG 6 cut(s) 367, 379, 1324, 1627, 3448, 4894
BspFNI CGCG 2 cut(s) 319, 4506
BspHI TCATGA 1 cut(s) 2343
BspLI GGNNCC 8 cut(s) 63, 374, 513, 636, 1633, 2121, 2196, 4302
BspMAI CTGCAG 6 cut(s) 436, 663, 705, 1215, 1327, 1451
BspMI ACCTGC 1 cut(s) 2563
BspOI GCTAGC 1 cut(s) 2976
BspPI GGATC 7 cut(s) 1079, 2037, 2275, 3132, 3743, 3956, 3957
BspQI GCTCTTC 1 cut(s) 195
BspT107I GGYRCC 2 cut(s) 634, 4300
BspTI CTTAAG 2 cut(s) 446, 611
BspTNI GGTCTC 1 cut(s) 1259
BsrBI CCGCTC 1 cut(s) 205
BsrDI GCAATG 8 cut(s) 495, 1605, 2625, 2718, 3085, 3732, 3993, 4612
BsrI ACTGG 5 cut(s) 868, 2075, 3403, 4327, 4387
BssNI GRCGYC 1 cut(s) 1565
BssSI CACGAG 1 cut(s) 1723
BssT1I CCWWGG 3 cut(s) 376, 3677, 4596
Bst2BI CACGAG 1 cut(s) 1723
Bst4CI ACNGT 8 cut(s) 82, 215, 940, 1503, 1965, 2124, 3346, 4041
Bst6I CTCTTC 6 cut(s) 174, 195, 498, 1190, 1845, 2641
BstACI GRCGYC 1 cut(s) 1565
BstAFI CTTAAG 2 cut(s) 446, 611
BstAPI GCANNNNNTGC 3 cut(s) 1018, 3377, 4796
BstDSI CCRYGG 1 cut(s) 3781
BstFNI CGCG 2 cut(s) 319, 4506
BstHHI GCGC 3 cut(s) 321, 2958, 4506
BstMAI GTCTC 7 cut(s) 1259, 1556, 1601, 2116, 3454, 3978, 4546
BstMCI CGRYCG 3 cut(s) 2852, 4462, 4510
BstNSI RCATGY 5 cut(s) 365, 1596, 2366, 3119, 3489
BstSFI CTRYAG 8 cut(s) 432, 659, 701, 1211, 1323, 1447, 3108, 3617
BstSLI GKGCMC 3 cut(s) 3382, 4019, 4305
BstUI CGCG 2 cut(s) 319, 4506
BstV2I GAAGAC 5 cut(s) 129, 1896, 3423, 3609, 4401
BstX2I RGATCY 5 cut(s) 1084, 2042, 2267, 2861, 3748
BstXI CCANNNNNNTGG 3 cut(s) 1642, 2179, 4677
BstYI RGATCY 5 cut(s) 1084, 2042, 2267, 2861, 3748
Bsu36I CCTNAGG 2 cut(s) 1614, 4176
BsuI GTATCC 1 cut(s) 4182
BtgI CCRYGG 1 cut(s) 3781
BtgZI GCGATG 1 cut(s) 1061
BtsI GCAGTG 1 cut(s) 2965
BtsIMutI CAGTG 2 cut(s) 220, 2965
BveI ACCTGC 1 cut(s) 2563
CaiI CAGNNNCTG 3 cut(s) 1331, 4292, 4716
CciI TCATGA 1 cut(s) 2343
CfoI GCGC 3 cut(s) 321, 2958, 4506
CseI GACGC 2 cut(s) 1260, 1573
Csp6I GTAC 8 cut(s) 861, 906, 936, 1148, 1927, 3356, 4140, 4683
CspCI CAANNNNNGTGG 2 cut(s) 4354, 4389
CviQI GTAC 8 cut(s) 861, 906, 936, 1148, 1927, 3356, 4140, 4683
DraI TTTAAA 1 cut(s) 1281
DraIII CACNNNGTG 1 cut(s) 1018
DrdI GACNNNNNNGTC 1 cut(s) 560
DseDI GACNNNNNNGTC 1 cut(s) 560
EaeI YGGCCR 2 cut(s) 3048, 3339
Eam1104I CTCTTC 6 cut(s) 174, 195, 498, 1190, 1845, 2641
EarI CTCTTC 6 cut(s) 174, 195, 498, 1190, 1845, 2641
Ecl136II GAGCTC 2 cut(s) 43, 289
Eco130I CCWWGG 3 cut(s) 376, 3677, 4596
Eco147I AGGCCT 3 cut(s) 963, 1420, 2754
Eco24I GRGCYC 5 cut(s) 45, 291, 1730, 2820, 4832
Eco31I GGTCTC 1 cut(s) 1259
Eco32I GATATC 2 cut(s) 3874, 4095
Eco47I GGWCC 8 cut(s) 373, 479, 1155, 2120, 2195, 2822, 4271, 4574
Eco53kI GAGCTC 2 cut(s) 43, 289
Eco57I CTGAAG 7 cut(s) 522, 1175, 1869, 3327, 3342, 4803, 4851
Eco81I CCTNAGG 2 cut(s) 1614, 4176
Eco88I CYCGRG 1 cut(s) 1523
EcoICRI GAGCTC 2 cut(s) 43, 289
EcoO109I RGGNCCY 3 cut(s) 373, 479, 2195
EcoRI GAATTC 4 cut(s) 840, 970, 3012, 3281
EcoRV GATATC 2 cut(s) 3874, 4095
EcoT14I CCWWGG 3 cut(s) 376, 3677, 4596
EcoT38I GRGCYC 5 cut(s) 45, 291, 1730, 2820, 4832
ErhI CCWWGG 3 cut(s) 376, 3677, 4596
Esp3I CGTCTC 1 cut(s) 1556
FalI AAGNNNNNCTT 6 cut(s) 1352, 1384, 1445, 1477, 3300, 3332
FaqI GGGAC 7 cut(s) 89, 386, 815, 998, 2133, 2208, 4126
FauI CCCGC 2 cut(s) 198, 324
FauNDI CATATG 2 cut(s) 1641, 4495
FbaI TGATCA 4 cut(s) 1108, 2299, 2350, 2545
FblI GTMKAC 3 cut(s) 84, 564, 3616
FriOI GRGCYC 5 cut(s) 45, 291, 1730, 2820, 4832
GlaI GCGC 3 cut(s) 320, 2957, 4505
GsaI CCCAGC 3 cut(s) 4681, 4720, 4744
GsuI CTGGAG 2 cut(s) 314, 1568
HapII CCGG 1 cut(s) 1886
HgaI GACGC 2 cut(s) 1260, 1573
HhaI GCGC 3 cut(s) 321, 2958, 4506
Hin1I GRCGYC 1 cut(s) 1565
Hin6I GCGC 3 cut(s) 319, 2956, 4504
HinP1I GCGC 3 cut(s) 319, 2956, 4504
HincII GTYRAC 3 cut(s) 85, 565, 2052
HindII GTYRAC 3 cut(s) 85, 565, 2052
HindIII AAGCTT 5 cut(s) 443, 608, 1758, 1859, 4662
HpaII CCGG 1 cut(s) 1886
Hpy166II GTNNAC 9 cut(s) 85, 565, 1354, 1722, 2052, 3380, 3617, 3817, 4424
Hpy8I GTNNAC 9 cut(s) 85, 565, 1354, 1722, 2052, 3380, 3617, 3817, 4424
Hpy99I CGWCG 8 cut(s) 26, 29, 50, 74, 107, 569, 1254, 4014
HpyCH4III ACNGT 8 cut(s) 82, 215, 940, 1503, 1965, 2124, 3346, 4041
HpyCH4IV ACGT 3 cut(s) 2308, 2329, 3843
HpySE526I ACGT 3 cut(s) 2308, 2329, 3843
Hsp92I GRCGYC 1 cut(s) 1565
HspAI GCGC 3 cut(s) 319, 2956, 4504
Ksp22I TGATCA 4 cut(s) 1108, 2299, 2350, 2545
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 5 cut(s) 294, 1631, 1692, 2288, 2378
LweI GCATC 9 cut(s) 1133, 1452, 1543, 1810, 1987, 3082, 3589, 4349, 4786
MaeII ACGT 3 cut(s) 2308, 2329, 3843
MaeIII GTNAC 8 cut(s) 1311, 1431, 1487, 1870, 2597, 2737, 3206, 3473
MbiI CCGCTC 1 cut(s) 205
MfeI CAATTG 2 cut(s) 771, 1752
MflI RGATCY 5 cut(s) 1084, 2042, 2267, 2861, 3748
MlsI TGGCCA 2 cut(s) 3050, 3341
MluNI TGGCCA 2 cut(s) 3050, 3341
MlyI GAGTC 4 cut(s) 1603, 3895, 4274, 4829
MmeI TCCRAC 5 cut(s) 45, 2443, 2979, 4033, 4358
Mox20I TGGCCA 2 cut(s) 3050, 3341
MroXI GAANNNNTTC 2 cut(s) 840, 1761
MscI TGGCCA 2 cut(s) 3050, 3341
MslI CAYNNNNRTG 5 cut(s) 1100, 1135, 1963, 4158, 4476
Msp20I TGGCCA 2 cut(s) 3050, 3341
MspA1I CMGCKG 3 cut(s) 706, 1978, 4716
MspCI CTTAAG 2 cut(s) 446, 611
MspI CCGG 1 cut(s) 1886
MunI CAATTG 2 cut(s) 771, 1752
MvnI CGCG 2 cut(s) 319, 4506
NdeI CATATG 2 cut(s) 1641, 4495
NheI GCTAGC 1 cut(s) 2972
NlaIV GGNNCC 8 cut(s) 63, 374, 513, 636, 1633, 2121, 2196, 4302
NmeAIII GCCGAG 1 cut(s) 4562
NmuCI GTSAC 4 cut(s) 1311, 1431, 3206, 3473
NspI RCATGY 5 cut(s) 365, 1596, 2366, 3119, 3489
OliI CACNNNNGTG 2 cut(s) 1135, 1963
PaeI GCATGC 1 cut(s) 365
PaeR7I CTCGAG 1 cut(s) 1523
PagI TCATGA 1 cut(s) 2343
PasI CCCWGGG 1 cut(s) 2172
PceI AGGCCT 3 cut(s) 963, 1420, 2754
PciI ACATGT 3 cut(s) 1592, 2362, 3115
PciSI GCTCTTC 1 cut(s) 195
PcsI WCGNNNNNNNCGW 4 cut(s) 45, 99, 123, 4006
PdmI GAANNNNTTC 2 cut(s) 840, 1761
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 291
Ple19I CGATCG 1 cut(s) 2852
PleI GAGTC 4 cut(s) 1603, 3895, 4273, 4829
PpsI GAGTC 4 cut(s) 1603, 3895, 4273, 4829
PpuMI RGGWCCY 3 cut(s) 373, 479, 2195
PscI ACATGT 3 cut(s) 1592, 2362, 3115
PshBI ATTAAT 1 cut(s) 3224
Psp124BI GAGCTC 2 cut(s) 45, 291
Psp5II RGGWCCY 3 cut(s) 373, 479, 2195
PspFI CCCAGC 3 cut(s) 4677, 4716, 4740
PspN4I GGNNCC 8 cut(s) 63, 374, 513, 636, 1633, 2121, 2196, 4302
PspPPI RGGWCCY 3 cut(s) 373, 479, 2195
PsrI GAACNNNNNNTAC 2 cut(s) 3509, 3541
PstI CTGCAG 6 cut(s) 436, 663, 705, 1215, 1327, 1451
PstNI CAGNNNCTG 3 cut(s) 1331, 4292, 4716
PsuI RGATCY 5 cut(s) 1084, 2042, 2267, 2861, 3748
PsyI GACNNNGTC 1 cut(s) 80
PvuI CGATCG 1 cut(s) 2852
PvuII CAGCTG 2 cut(s) 706, 4716
RsaI GTAC 8 cut(s) 862, 907, 937, 1149, 1928, 3357, 4141, 4684
RsaNI GTAC 8 cut(s) 861, 906, 936, 1148, 1927, 3356, 4140, 4683
RseI CAYNNNNRTG 5 cut(s) 1100, 1135, 1963, 4158, 4476
SacI GAGCTC 2 cut(s) 45, 291
SalI GTCGAC 2 cut(s) 83, 563
SapI GCTCTTC 1 cut(s) 195
ScaI AGTACT 2 cut(s) 862, 1928
SchI GAGTC 4 cut(s) 1603, 3895, 4274, 4829
SfaNI GCATC 9 cut(s) 1133, 1452, 1543, 1810, 1987, 3082, 3589, 4349, 4786
SfcI CTRYAG 8 cut(s) 432, 659, 701, 1211, 1323, 1447, 3108, 3617
Sfr274I CTCGAG 1 cut(s) 1523
SinI GGWCC 8 cut(s) 373, 479, 1155, 2120, 2195, 2822, 4271, 4574
SlaI CTCGAG 1 cut(s) 1523
SmiMI CAYNNNNRTG 5 cut(s) 1100, 1135, 1963, 4158, 4476
SmlI CTYRAG 4 cut(s) 446, 488, 611, 1523
SmoI CTYRAG 4 cut(s) 446, 488, 611, 1523
SpeI ACTAGT 1 cut(s) 2054
SphI GCATGC 1 cut(s) 365
SseBI AGGCCT 3 cut(s) 963, 1420, 2754
SspI AATATT 3 cut(s) 2874, 3574, 3709
SstI GAGCTC 2 cut(s) 45, 291
StuI AGGCCT 3 cut(s) 963, 1420, 2754
StyI CCWWGG 3 cut(s) 376, 3677, 4596
TaaI ACNGT 8 cut(s) 82, 215, 940, 1503, 1965, 2124, 3346, 4041
TaiI ACGT 3 cut(s) 2311, 2332, 3846
TatI WGTACW 3 cut(s) 860, 1926, 4139
TauI GCSGC 6 cut(s) 99, 102, 343, 4899, 4902, 4905
TscAI CASTG 2 cut(s) 220, 2965
TseFI GTSAC 4 cut(s) 1311, 1431, 3206, 3473
Tsp45I GTSAC 4 cut(s) 1311, 1431, 3206, 3473
TspGWI ACGGA 5 cut(s) 549, 2103, 2146, 3878, 4150
TspRI CASTG 2 cut(s) 220, 2965
Tth111I GACNNNGTC 1 cut(s) 80
Vha464I CTTAAG 2 cut(s) 446, 611
VneI GTGCAC 1 cut(s) 3378
VpaK11BI GGWCC 8 cut(s) 373, 479, 1155, 2120, 2195, 2822, 4271, 4574
VspI ATTAAT 1 cut(s) 3224
XapI RAATTY 9 cut(s) 332, 572, 761, 840, 970, 2764, 3012, 3281, 4004
XceI RCATGY 5 cut(s) 365, 1596, 2366, 3119, 3489
XcmI CCANNNNNNNNNTGG 1 cut(s) 4160
XhoI CTCGAG 1 cut(s) 1523
XmiI GTMKAC 3 cut(s) 84, 564, 3616
XmnI GAANNNNTTC 2 cut(s) 840, 1761
ZrmI AGTACT 2 cut(s) 862, 1928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.