Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010271

Physical Location & Seq

Genomic Coordinates Forward (+)

24778257 .. 24780742

Gene Structure 2486 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G66430.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1155 bp

ATGGCTCTCCAAGCCACTACTACTACGTTCTGCTTCTCCGGTCCAACTTTCCGATCAACTCCTCACTCACTCACTTCAAAACGTCCTATCTCCATCAAAGCTACTACTTCTTCTCCTTCAAGACTCTCCAATTCACGATCTAATCTCAAAGGAAGAGCATTGTCTAGTGATGGTTCAACACAAGAATCTCCTTATGTAGTCTGTTTTGGAGAAATGCTAATCGATTTTGTACCGACGACAAGCGGACTTTCATTAGCCGATGCACCGGCTTTTAAAAAAGCTCCAGGTGGTGCACCTGCTAATGTAGCTGTTGGTATTGCTCGTCTTGGTGGTTCTTCAGCTTTCATTGGAAAGGTTGGTGAAGATGAGTTTGGTTATATGCTTGCGAATATTTTGAAAGATAATAATGTGAACAATGATGGTATGAGGTTTGATCCAGGAGCAAGAACTGCTTTAGCTTTTGTGACTTTGACTAATGAAGGTGAAAGAGAGTTTATGTTTTACCGAAACCCGAGTGCTGATATGTTGTTAGAGGAGTCTGAGCTTGATTTTGATTTGATCAAAAAGGCAAAGATATTTCATTACGGTTCGATAAGTCTTATCACCGAACCGTGCAAGTCTGCGCATATTTCTGCAGCTAAGGCGGCTAAGGAGGCTGGTGTTATACTCTCTTATGACCCTAACCTTAGGCTTCCGTTGTGGCCTTCTGCAGACAATGCTAGGGAAGAGATTCTGAGCATTTGGGAGACTGCTGATATTATAAAGATAAGTGAAGAGGAGATTGTCTTTCTAACGAAAGGAGAAGATCCGTATGATGATAATGTTGTACGCAAGTTGTTTCATCCTAAGCTGAAACTTCTCCTTGTCACTGAAGGTCCTGAAGGCTGTCGGTACTACACCAAGGATTTTAGTGGGAGAGTGCATGGATTGAAGGTGGATGTAGTGGACACAACAGGTGCTGGTGATGCATTTGTTGCGGGGATACTATCGCAACTAGCCAATGATCTCTCCTTGCTTCAGGACGAAGAGCGACTGAGAGAGGCTCTTATGTTTGCGAATGCATGCGGGGCATTAACCGTTAAAGTGAGAGGCGCAATCCCGGCCCTTCCAACCAAAGAAGCTGTACATGAGGCTCTACTCAAAGCCGTCGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004396 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0005975 GO:0005996 GO:0006000 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008865 GO:0009058 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016051 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0019318 GO:0019752 GO:0030054 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044262 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046835 GO:0055044 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00051 ko00500 ko00520 ko01100 map00051 map00500 map00520 map01100

Pfam Domains

Protein Families

PfkB

Protein Analysis

384

Amino Acids

41.47

Weight (kDa)

5.54

Isoelectric Point (pI)

37.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	65 - 371	9.7e-79	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010271)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66430
fragaria_vesca	FvH4_1g28890 FvH4_1g28890 FvH4_1g28890 FvH4_1g28890
malus_domestica	MD09G1262800.v1.1 MD17G1257700.v1.1
prunus_persica	Prupe.3G160500_v2.0.a1 Prupe.3G160500_v2.0.a1
pyrus_communis	pycom09g17710 pycom17g25980
rosa_chinensis	RchiOBHm_Chr3g0493391
rosa_laevigata	RLG00000022716
rosa_multiflora	Rmu_sc0000828.1_g000020 Rmu_sc0010338.1_g000012
rosa_roxburghii	Rroxscaffold_6G00391620
rosa_rugosa	Rorug03G0260700
rosa_samantha	Rh3AG309800 Rh3CG343400 Rh3DG345000
rosa_wichuraiana	Rw3G027210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	761
AarI	CACCTGC	1 cut(s)	304
Acc16I	TGCGCA	1 cut(s)	624
Acc36I	ACCTGC	1 cut(s)	304
AciI	CCGC	4 cut(s)	243, 644, 977, 1065
AclWI	GGATC	2 cut(s)	428, 800
AcuI	CTGAAG	4 cut(s)	321, 891, 900, 1001
AfaI	GTAC	4 cut(s)	231, 828, 893, 1125
AgsI	TTSAA	5 cut(s)	78, 120, 177, 397, 931
AjnI	CCWGG	2 cut(s)	283, 436
AjuI	GAANNNNNNNTTGG	2 cut(s)	354, 386
AluBI	AGCT	9 cut(s)	101, 281, 308, 341, 458, 544, 638, 850, 1121
AluI	AGCT	9 cut(s)	101, 281, 308, 341, 458, 544, 638, 850, 1121
Alw21I	GWGCWC	1 cut(s)	295
Alw26I	GTCTC	1 cut(s)	740
Alw44I	GTGCAC	1 cut(s)	291
AlwI	GGATC	2 cut(s)	428, 800
AlwNI	CAGNNNCTG	1 cut(s)	959
Ama87I	CYCGRG	1 cut(s)	511
AoxI	GGCC	2 cut(s)	701, 1101
ApaLI	GTGCAC	1 cut(s)	291
ApeKI	GCWGC	1 cut(s)	635
Asp700I	GAANNNNTTC	1 cut(s)	729
AspLEI	GCGC	2 cut(s)	625, 1094
AspS9I	GGNCC	3 cut(s)	41, 875, 1102
AsuC2I	CCSGG	1 cut(s)	1100
AsuHPI	GGTGA	4 cut(s)	371, 494, 595, 974
AvaI	CYCGRG	1 cut(s)	511
AvaII	GGWCC	2 cut(s)	41, 875
AxyI	CCTNAGG	1 cut(s)	686
BaeGI	GKGCMC	1 cut(s)	295
Bbv12I	GWGCWC	1 cut(s)	295
BbvI	GCAGC	1 cut(s)	647
BccI	CCATC	3 cut(s)	101, 164, 413
BceAI	ACGGC	1 cut(s)	1130
BciT130I	CCWGG	2 cut(s)	285, 438
BciVI	GTATCC	1 cut(s)	975
BclI	TGATCA	1 cut(s)	558
BcnI	CCSGG	1 cut(s)	1100
BcoDI	GTCTC	1 cut(s)	740
BfaI	CTAG	3 cut(s)	165, 720, 995
BfmI	CTRYAG	2 cut(s)	633, 708
BfuAI	ACCTGC	1 cut(s)	304
BfuI	GTATCC	1 cut(s)	975
BisI	GCNGC	2 cut(s)	636, 645
BlsI	GCNGC	2 cut(s)	637, 646
Bme1390I	CCNGG	3 cut(s)	285, 438, 1100
Bme18I	GGWCC	2 cut(s)	41, 875
BmeT110I	CYCGRG	1 cut(s)	511
BmgT120I	GGNCC	3 cut(s)	41, 875, 1102
BmrFI	CCNGG	3 cut(s)	285, 438, 1100
BmsI	GCATC	2 cut(s)	250, 955
BplI	GAGNNNNNCTC	2 cut(s)	1027, 1059
BpmI	CTGGAG	1 cut(s)	267
Bpu10I	CCTNAGC	3 cut(s)	639, 648, 846
BpuMI	CCSGG	1 cut(s)	1100
Bsa29I	ATCGAT	1 cut(s)	222
BsaJI	CCNNGG	1 cut(s)	900
BsaWI	WCCGGW	1 cut(s)	38
BsaXI	ACNNNNNCTCC	2 cut(s)	97, 127
Bse118I	RCCGGY	1 cut(s)	265
Bse21I	CCTNAGG	1 cut(s)	686
BseBI	CCWGG	2 cut(s)	285, 438
BseCI	ATCGAT	1 cut(s)	222
BseDI	CCNNGG	1 cut(s)	900
BseGI	GGATG	2 cut(s)	841, 943
BseMII	CTCAG	3 cut(s)	531, 725, 1025
BseRI	GAGGAG	3 cut(s)	51, 548, 791
BseSI	GKGCMC	1 cut(s)	295
BseXI	GCAGC	1 cut(s)	647
BshFI	GGCC	2 cut(s)	703, 1103
BshVI	ATCGAT	1 cut(s)	222
BsiHKAI	GWGCWC	1 cut(s)	295
BsiHKCI	CYCGRG	1 cut(s)	511
BsiSI	CCGG	3 cut(s)	39, 266, 1100
BsmAI	GTCTC	1 cut(s)	740
BsmI	GAATGC	1 cut(s)	1063
BsnI	GGCC	2 cut(s)	703, 1103
BsoBI	CYCGRG	1 cut(s)	511
Bsp1286I	GDGCHC	1 cut(s)	295
Bsp1407I	TGTACA	1 cut(s)	1123
Bsp143I	GATC	6 cut(s)	53, 137, 433, 558, 805, 1003
BspACI	CCGC	4 cut(s)	243, 644, 977, 1065
BspANI	GGCC	2 cut(s)	703, 1103
BspCNI	CTCAG	3 cut(s)	532, 726, 1026
BspDI	ATCGAT	1 cut(s)	222
BspMAI	CTGCAG	2 cut(s)	637, 712
BspMI	ACCTGC	1 cut(s)	304
BspPI	GGATC	2 cut(s)	428, 800
BspQI	GCTCTTC	2 cut(s)	148, 1020
BsrFI	RCCGGY	1 cut(s)	265
BsrGI	TGTACA	1 cut(s)	1123
BssAI	RCCGGY	1 cut(s)	265
BssECI	CCNNGG	1 cut(s)	900
BssMI	GATC	6 cut(s)	53, 137, 433, 558, 805, 1003
BssT1I	CCWWGG	1 cut(s)	900
Bst2UI	CCWGG	2 cut(s)	285, 438
Bst4CI	ACNGT	3 cut(s)	587, 612, 1078
Bst6I	CTCTTC	4 cut(s)	148, 720, 768, 1020
BstAPI	GCANNNNNTGC	3 cut(s)	449, 716, 974
BstAUI	TGTACA	1 cut(s)	1123
BstC8I	GCNNGC	2 cut(s)	384, 1063
BstDEI	CTNAG	7 cut(s)	540, 639, 648, 686, 734, 846, 1034
BstF5I	GGATG	2 cut(s)	841, 943
BstHHI	GCGC	2 cut(s)	625, 1094
BstKTI	GATC	6 cut(s)	56, 140, 436, 561, 808, 1006
BstMAI	GTCTC	1 cut(s)	740
BstMBI	GATC	6 cut(s)	53, 137, 433, 558, 805, 1003
BstNI	CCWGG	2 cut(s)	285, 438
BstNSI	RCATGY	1 cut(s)	1065
BstSCI	CCNGG	3 cut(s)	283, 436, 1098
BstSFI	CTRYAG	2 cut(s)	633, 708
BstSLI	GKGCMC	1 cut(s)	295
BstV1I	GCAGC	1 cut(s)	647
BstX2I	RGATCY	1 cut(s)	805
BstYI	RGATCY	1 cut(s)	805
Bsu15I	ATCGAT	1 cut(s)	222
Bsu36I	CCTNAGG	1 cut(s)	686
BsuI	GTATCC	1 cut(s)	975
BsuRI	GGCC	2 cut(s)	703, 1103
BsuTUI	ATCGAT	1 cut(s)	222
BtsCI	GGATG	2 cut(s)	841, 943
BtsIMutI	CAGTG	1 cut(s)	867
BveI	ACCTGC	1 cut(s)	304
Cac8I	GCNNGC	2 cut(s)	384, 1063
CaiI	CAGNNNCTG	1 cut(s)	959
CfoI	GCGC	2 cut(s)	625, 1094
Cfr10I	RCCGGY	1 cut(s)	265
Cfr13I	GGNCC	3 cut(s)	41, 875, 1102
ClaI	ATCGAT	1 cut(s)	222
Csp6I	GTAC	4 cut(s)	230, 827, 892, 1124
CviAII	CATG	3 cut(s)	923, 1062, 1127
CviQI	GTAC	4 cut(s)	230, 827, 892, 1124
DdeI	CTNAG	7 cut(s)	540, 639, 648, 686, 734, 846, 1034
DpnI	GATC	6 cut(s)	55, 139, 435, 560, 807, 1005
DpnII	GATC	6 cut(s)	53, 137, 433, 558, 805, 1003
DraI	TTTAAA	1 cut(s)	274
Eam1104I	CTCTTC	4 cut(s)	148, 720, 768, 1020
EarI	CTCTTC	4 cut(s)	148, 720, 768, 1020
Eco130I	CCWWGG	1 cut(s)	900
Eco47I	GGWCC	2 cut(s)	41, 875
Eco57I	CTGAAG	4 cut(s)	321, 891, 900, 1001
Eco81I	CCTNAGG	1 cut(s)	686
Eco88I	CYCGRG	1 cut(s)	511
EcoO109I	RGGNCCY	1 cut(s)	875
EcoRII	CCWGG	2 cut(s)	283, 436
EcoT14I	CCWWGG	1 cut(s)	900
EcoT22I	ATGCAT	2 cut(s)	970, 1063
ErhI	CCWWGG	1 cut(s)	900
FaeI	CATG	3 cut(s)	926, 1065, 1130
FalI	AAGNNNNNCTT	2 cut(s)	436, 468
FatI	CATG	3 cut(s)	922, 1061, 1126
FauI	CCCGC	2 cut(s)	970, 1058
FbaI	TGATCA	1 cut(s)	558
Fnu4HI	GCNGC	2 cut(s)	636, 645
FokI	GGATG	2 cut(s)	828, 950
Fsp4HI	GCNGC	2 cut(s)	636, 645
FspBI	CTAG	3 cut(s)	165, 720, 995
FspI	TGCGCA	1 cut(s)	624
GlaI	GCGC	2 cut(s)	624, 1093
GluI	GCNGC	2 cut(s)	636, 645
GsuI	CTGGAG	1 cut(s)	267
HaeIII	GGCC	2 cut(s)	703, 1103
HapII	CCGG	3 cut(s)	39, 266, 1100
HhaI	GCGC	2 cut(s)	625, 1094
Hin1II	CATG	3 cut(s)	926, 1065, 1130
Hin6I	GCGC	2 cut(s)	623, 1092
HinP1I	GCGC	2 cut(s)	623, 1092
HinfI	GANTC	4 cut(s)	123, 185, 536, 730
HpaII	CCGG	3 cut(s)	39, 266, 1100
HphI	GGTGA	4 cut(s)	371, 494, 595, 974
Hpy166II	GTNNAC	3 cut(s)	293, 412, 946
Hpy188I	TCNGA	3 cut(s)	53, 541, 735
Hpy188III	TCNNGA	4 cut(s)	120, 135, 878, 1019
Hpy8I	GTNNAC	3 cut(s)	293, 412, 946
Hpy99I	CGWCG	2 cut(s)	238, 1151
HpyAV	CCTTC	7 cut(s)	126, 473, 714, 866, 875, 925, 1115
HpyCH4III	ACNGT	3 cut(s)	587, 612, 1078
HpyCH4IV	ACGT	2 cut(s)	26, 82
HpyCH4V	TGCA	8 cut(s)	263, 293, 615, 635, 710, 922, 968, 1061
HpyF3I	CTNAG	7 cut(s)	540, 639, 648, 686, 734, 846, 1034
HpySE526I	ACGT	2 cut(s)	26, 82
Hsp92II	CATG	3 cut(s)	926, 1065, 1130
HspAI	GCGC	2 cut(s)	623, 1092
Ksp22I	TGATCA	1 cut(s)	558
Kzo9I	GATC	6 cut(s)	53, 137, 433, 558, 805, 1003
LguI	GCTCTTC	2 cut(s)	148, 1020
LmnI	GCTCC	2 cut(s)	286, 440
Lsp1109I	GCAGC	1 cut(s)	647
LweI	GCATC	2 cut(s)	250, 955
MaeI	CTAG	3 cut(s)	165, 720, 995
MaeII	ACGT	2 cut(s)	26, 82
MaeIII	GTNAC	2 cut(s)	463, 865
MalI	GATC	6 cut(s)	55, 139, 435, 560, 807, 1005
MboI	GATC	6 cut(s)	53, 137, 433, 558, 805, 1003
MboII	GAAGA	8 cut(s)	102, 165, 327, 374, 737, 785, 815, 1037
MflI	RGATCY	1 cut(s)	805
MhlI	GDGCHC	1 cut(s)	295
MluCI	AATT	1 cut(s)	130
MlyI	GAGTC	2 cut(s)	117, 545
MmeI	TCCRAC	2 cut(s)	68, 1133
MnlI	CCTC	8 cut(s)	72, 420, 526, 646, 769, 1033, 1082, 1123
Mph1103I	ATGCAT	2 cut(s)	970, 1063
MroXI	GAANNNNTTC	1 cut(s)	729
MseI	TTAA	4 cut(s)	273, 1073, 1080, 1153
MspI	CCGG	3 cut(s)	39, 266, 1100
MspR9I	CCNGG	3 cut(s)	285, 438, 1100
Mva1269I	GAATGC	1 cut(s)	1063
MvaI	CCWGG	2 cut(s)	285, 438
NciI	CCSGG	1 cut(s)	1100
NdeII	GATC	6 cut(s)	53, 137, 433, 558, 805, 1003
NlaIII	CATG	3 cut(s)	926, 1065, 1130
NmuCI	GTSAC	2 cut(s)	463, 865
NsbI	TGCGCA	1 cut(s)	624
NsiI	ATGCAT	2 cut(s)	970, 1063
NspI	RCATGY	1 cut(s)	1065
PaeI	GCATGC	1 cut(s)	1065
PaqCI	CACCTGC	1 cut(s)	304
PciSI	GCTCTTC	2 cut(s)	148, 1020
PctI	GAATGC	1 cut(s)	1063
PdmI	GAANNNNTTC	1 cut(s)	729
PfeI	GAWTC	2 cut(s)	185, 730
PfoI	TCCNGGA	1 cut(s)	436
PkrI	GCNGC	2 cut(s)	637, 646
PleI	GAGTC	2 cut(s)	117, 544
PpsI	GAGTC	2 cut(s)	117, 544
PpuMI	RGGWCCY	1 cut(s)	875
PsiI	TTATAA	1 cut(s)	761
Psp5II	RGGWCCY	1 cut(s)	875
Psp6I	CCWGG	2 cut(s)	283, 436
PspGI	CCWGG	2 cut(s)	283, 436
PspPI	GGNCC	3 cut(s)	41, 875, 1102
PspPPI	RGGWCCY	1 cut(s)	875
PstI	CTGCAG	2 cut(s)	637, 712
PstNI	CAGNNNCTG	1 cut(s)	959
PsuI	RGATCY	1 cut(s)	805
RsaI	GTAC	4 cut(s)	231, 828, 893, 1125
RsaNI	GTAC	4 cut(s)	230, 827, 892, 1124
SapI	GCTCTTC	2 cut(s)	148, 1020
SaqAI	TTAA	4 cut(s)	273, 1073, 1080, 1153
SatI	GCNGC	2 cut(s)	636, 645
Sau3AI	GATC	6 cut(s)	53, 137, 433, 558, 805, 1003
Sau96I	GGNCC	3 cut(s)	41, 875, 1102
SchI	GAGTC	2 cut(s)	117, 545
ScrFI	CCNGG	3 cut(s)	285, 438, 1100
SduI	GDGCHC	1 cut(s)	295
SfaNI	GCATC	2 cut(s)	250, 955
SfcI	CTRYAG	2 cut(s)	633, 708
SinI	GGWCC	2 cut(s)	41, 875
SphI	GCATGC	1 cut(s)	1065
Sse9I	AATT	1 cut(s)	130
SsiI	CCGC	4 cut(s)	243, 644, 977, 1065
SspI	AATATT	1 cut(s)	391
SspMI	CTAG	3 cut(s)	165, 720, 995
StyD4I	CCNGG	3 cut(s)	283, 436, 1098
StyI	CCWWGG	1 cut(s)	900
TaaI	ACNGT	3 cut(s)	587, 612, 1078
TaiI	ACGT	2 cut(s)	29, 85
TaqI	TCGA	2 cut(s)	222, 590
TasI	AATT	1 cut(s)	130
TatI	WGTACW	1 cut(s)	1123
TauI	GCSGC	1 cut(s)	647
TfiI	GAWTC	2 cut(s)	185, 730
Tru1I	TTAA	4 cut(s)	273, 1073, 1080, 1153
Tru9I	TTAA	4 cut(s)	273, 1073, 1080, 1153
TscAI	CASTG	1 cut(s)	874
TseFI	GTSAC	2 cut(s)	463, 865
TseI	GCWGC	1 cut(s)	635
Tsp45I	GTSAC	2 cut(s)	463, 865
TspDTI	ATGAA	5 cut(s)	240, 334, 492, 569, 830
TspGWI	ACGGA	2 cut(s)	684, 798
TspRI	CASTG	1 cut(s)	874
VneI	GTGCAC	1 cut(s)	291
VpaK11BI	GGWCC	2 cut(s)	41, 875
XceI	RCATGY	1 cut(s)	1065
XmnI	GAANNNNTTC	1 cut(s)	729
XspI	CTAG	3 cut(s)	165, 720, 995
Zsp2I	ATGCAT	2 cut(s)	970, 1063

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.