Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0010271

Physical Location & Seq

Genomic Coordinates Reverse (-)

31819740 .. 31825304

Gene Structure 5565 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1257700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGGCTCTTCATTCTACTGCTTTCTGCTTCGGCGGGGTGGTTTCTTTGCCTCGCAATTCAGTTTCTTTTAGTCAACGGACAGTCAGAGCCTCTGCATTTGCTTCCCCACCACCTCTCAGTTCTTCCTCCATTGCCAGATCGAATGTCCAAGGAAAAGCATTTGCAGGAGATGTGCTATCGGACACAAAAGAATCCTCCCTTGTGGTTTGTTTCGGGGAAATGCTGATTGATTTTGTCCCAACTACTAATGGACTTTCATTGGCTGAAGCACCTGCATTTAAAAAAGCTGCTGGAGGAGCCCCTGCTAATGTTGCAGTTGGCATAGCTCGTCTTGGTGGCTCATCAGCTTTTATTGGAAAGGTCGGGGAAGATGAATTTGGATACATGCTTGCTGATATACTAAAGGAGAATAATGTGAACAATGAGGGATTGCGTTTTGATCCTGGTGCACGAACTGCTTTAGCATTTGTTACATTGAGGAGTGATGGGGAACGTGAGTTCATGTTTTATCGTAACCCTAGTGCTGATATGTTGCTTCAAGAAGCTGAACTTGATTTTGATTTAATTAGGAAGGCAAAAATATTACATTATGGTTCTATAAGTCTTATCACGGAACCATGCAAGTCGGCTCATATTGCAGCAACAAAAGCTGCAAAGGACGCTGGTGTAGTTCTGTCCTACGATCCCAACCTCAGGCTTCCACTGTGGCCTTCCGCAAAGAGTGCTAGAGAAGGAATTTTGAGTATATGGGACTCTGCTGATGTTATCAAGATAAGTGAAGAAGAGATTTCTTTTCTAACGGAAGGAGAAGATCCATATGATGAAAATGTTGTTCGAAAATTGTACCACCCAAATCTTAAATTACTTCTGGTCACTGAGGGCCCTGATGGTTGCAGGTACTACACCAAGGAATTCAGTGGAAGAGTCAAGGGTATGAAGGTAGATGTAGTGGACACCACTGGTGCTGGGGACGCATTTGTGGCTGGAATATTATCACAGCTAGCTGTTGATCTTTCTTTGCTTCAGGACGAGGACAAACTGAGAGATGCGCTCCTGTTTGCCAACGCTTGCGGCGCATTGACTGTGATGGGGAGAGGTGCTATTCCGGCTTTGCCAACTCGAGAATCGGTGTTGAATGTCCTTCTCAAGTCTGTAGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004396 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0005975 GO:0005996 GO:0006000 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008610 GO:0008865 GO:0009058 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016051 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0019318 GO:0019752 GO:0030054 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044262 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046835 GO:0055044 GO:0071704 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00051 ko00500 ko00520 ko01100 map00051 map00500 map00520 map01100

Pfam Domains

Protein Families

PfkB

Protein Analysis

387

Amino Acids

41.23

Weight (kDa)

5.24

Isoelectric Point (pI)

34.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	67 - 373	2e-75	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010271)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66430
fragaria_vesca	FvH4_1g28890 FvH4_1g28890 FvH4_1g28890 FvH4_1g28890
malus_domestica	MD09G1262800.v1.1 MD17G1257700.v1.1
prunus_persica	Prupe.3G160500_v2.0.a1 Prupe.3G160500_v2.0.a1
pyrus_communis	pycom09g17710 pycom17g25980
rosa_chinensis	RchiOBHm_Chr3g0493391
rosa_laevigata	RLG00000022716
rosa_multiflora	Rmu_sc0000828.1_g000020 Rmu_sc0010338.1_g000012
rosa_roxburghii	Rroxscaffold_6G00391620
rosa_rugosa	Rorug03G0260700
rosa_samantha	Rh3AG309800 Rh3CG343400 Rh3DG345000
rosa_wichuraiana	Rw3G027210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	280
Acc36I	ACCTGC	2 cut(s)	280, 885
AciI	CCGC	3 cut(s)	33, 714, 1071
AclWI	GGATC	3 cut(s)	434, 677, 806
AcsI	RAATTY	3 cut(s)	374, 735, 911
AcuI	CTGAAG	2 cut(s)	285, 1007
AfaI	GTAC	2 cut(s)	845, 899
AfiI	CCNNNNNNNGG	1 cut(s)	693
AgsI	TTSAA	2 cut(s)	539, 1135
AjnI	CCWGG	1 cut(s)	442
AjuI	GAANNNNNNNTTGG	2 cut(s)	360, 392
AloI	GAACNNNNNNTCC	2 cut(s)	482, 514
AluBI	AGCT	7 cut(s)	287, 326, 347, 545, 650, 1000, 1004
AluI	AGCT	7 cut(s)	287, 326, 347, 545, 650, 1000, 1004
Alw21I	GWGCWC	1 cut(s)	451
Alw44I	GTGCAC	1 cut(s)	447
AlwI	GGATC	3 cut(s)	434, 677, 806
Ama87I	CYCGRG	1 cut(s)	1119
AoxI	GGCC	2 cut(s)	707, 880
ApaI	GGGCCC	1 cut(s)	884
ApaLI	GTGCAC	1 cut(s)	447
ApeKI	GCWGC	3 cut(s)	287, 638, 650
ApoI	RAATTY	3 cut(s)	374, 735, 911
AspLEI	GCGC	2 cut(s)	1051, 1076
AspS9I	GGNCC	2 cut(s)	880, 881
AsuII	TTCGAA	1 cut(s)	835
AsuNHI	GCTAGC	1 cut(s)	1000
AvaI	CYCGRG	1 cut(s)	1119
AxyI	CCTNAGG	1 cut(s)	692
BaeGI	GKGCMC	2 cut(s)	451, 884
BanII	GRGCYC	2 cut(s)	301, 884
Bbv12I	GWGCWC	1 cut(s)	451
BbvI	GCAGC	3 cut(s)	274, 637, 650
BccI	CCATC	3 cut(s)	479, 881, 1081
BciT130I	CCWGG	1 cut(s)	444
BciVI	GTATCC	1 cut(s)	374
BfaI	CTAG	4 cut(s)	519, 726, 1001, 1159
BfmI	CTRYAG	1 cut(s)	1152
BfuAI	ACCTGC	2 cut(s)	280, 885
BfuI	GTATCC	1 cut(s)	374
BisI	GCNGC	4 cut(s)	288, 639, 651, 1072
BlsI	GCNGC	4 cut(s)	289, 640, 652, 1073
Bme1390I	CCNGG	1 cut(s)	444
BmeT110I	CYCGRG	1 cut(s)	1119
BmgT120I	GGNCC	2 cut(s)	880, 881
BmiI	GGNNCC	3 cut(s)	298, 615, 882
BmrFI	CCNGG	1 cut(s)	444
BmsI	GCATC	1 cut(s)	1036
BmtI	GCTAGC	1 cut(s)	1004
BplI	GAGNNNNNCTC	2 cut(s)	1035, 1067
BpmI	CTGGAG	1 cut(s)	312
Bpu14I	TTCGAA	1 cut(s)	835
BpuEI	CTTGAG	1 cut(s)	1130
BsaJI	CCNNGG	2 cut(s)	148, 906
Bsc4I	CCNNNNNNNGG	1 cut(s)	693
Bse1I	ACTGG	1 cut(s)	964
Bse21I	CCTNAGG	1 cut(s)	692
Bse3DI	GCAATG	1 cut(s)	129
BseBI	CCWGG	1 cut(s)	444
BseDI	CCNNGG	2 cut(s)	148, 906
BseLI	CCNNNNNNNGG	1 cut(s)	693
BseMI	GCAATG	1 cut(s)	129
BseMII	CTCAG	4 cut(s)	130, 706, 867, 1031
BseNI	ACTGG	1 cut(s)	964
BseRI	GAGGAG	2 cut(s)	309, 493
BseSI	GKGCMC	2 cut(s)	451, 884
BseXI	GCAGC	3 cut(s)	274, 637, 650
BseYI	CCCAGC	1 cut(s)	965
BshFI	GGCC	2 cut(s)	709, 882
BsiHKAI	GWGCWC	1 cut(s)	451
BsiHKCI	CYCGRG	1 cut(s)	1119
BsiSI	CCGG	1 cut(s)	1106
BslFI	GGGAC	3 cut(s)	221, 764, 983
BslI	CCNNNNNNNGG	1 cut(s)	693
BsmFI	GGGAC	3 cut(s)	221, 764, 983
BsnI	GGCC	2 cut(s)	709, 882
BsoBI	CYCGRG	1 cut(s)	1119
Bsp119I	TTCGAA	1 cut(s)	835
Bsp120I	GGGCCC	1 cut(s)	880
Bsp1286I	GDGCHC	3 cut(s)	301, 451, 884
Bsp143I	GATC	5 cut(s)	137, 439, 682, 811, 1009
BspACI	CCGC	3 cut(s)	33, 714, 1071
BspANI	GGCC	2 cut(s)	709, 882
BspCNI	CTCAG	4 cut(s)	129, 705, 868, 1032
BspLI	GGNNCC	3 cut(s)	298, 615, 882
BspMI	ACCTGC	2 cut(s)	280, 885
BspOI	GCTAGC	1 cut(s)	1004
BspPI	GGATC	3 cut(s)	434, 677, 806
BspQI	GCTCTTC	1 cut(s)	12
BspT104I	TTCGAA	1 cut(s)	835
BsrDI	GCAATG	1 cut(s)	129
BsrI	ACTGG	1 cut(s)	964
BssECI	CCNNGG	2 cut(s)	148, 906
BssMI	GATC	5 cut(s)	137, 439, 682, 811, 1009
BssT1I	CCWWGG	2 cut(s)	148, 906
Bst2UI	CCWGG	1 cut(s)	444
Bst4CI	ACNGT	3 cut(s)	82, 705, 1084
Bst6I	CTCTTC	3 cut(s)	12, 777, 916
BstAPI	GCANNNNNTGC	2 cut(s)	455, 722
BstBI	TTCGAA	1 cut(s)	835
BstC8I	GCNNGC	3 cut(s)	390, 1002, 1069
BstDEI	CTNAG	4 cut(s)	116, 692, 876, 1040
BstHHI	GCGC	2 cut(s)	1051, 1076
BstKTI	GATC	5 cut(s)	140, 442, 685, 814, 1012
BstMBI	GATC	5 cut(s)	137, 439, 682, 811, 1009
BstNI	CCWGG	1 cut(s)	444
BstNSI	RCATGY	1 cut(s)	388
BstSCI	CCNGG	1 cut(s)	442
BstSFI	CTRYAG	1 cut(s)	1152
BstSLI	GKGCMC	2 cut(s)	451, 884
BstV1I	GCAGC	3 cut(s)	274, 637, 650
BstX2I	RGATCY	1 cut(s)	811
BstYI	RGATCY	1 cut(s)	811
Bsu36I	CCTNAGG	1 cut(s)	692
BsuI	GTATCC	1 cut(s)	374
BsuRI	GGCC	2 cut(s)	709, 882
BtsIMutI	CAGTG	4 cut(s)	701, 873, 922, 957
BveI	ACCTGC	2 cut(s)	280, 885
Cac8I	GCNNGC	3 cut(s)	390, 1002, 1069
CfoI	GCGC	2 cut(s)	1051, 1076
Cfr13I	GGNCC	2 cut(s)	880, 881
CseI	GACGC	2 cut(s)	668, 980
Csp6I	GTAC	2 cut(s)	844, 898
CviAII	CATG	3 cut(s)	385, 502, 618
CviQI	GTAC	2 cut(s)	844, 898
DdeI	CTNAG	4 cut(s)	116, 692, 876, 1040
DpnI	GATC	5 cut(s)	139, 441, 684, 813, 1011
DpnII	GATC	5 cut(s)	137, 439, 682, 811, 1009
DraI	TTTAAA	1 cut(s)	280
Eam1104I	CTCTTC	3 cut(s)	12, 777, 916
EarI	CTCTTC	3 cut(s)	12, 777, 916
Eco130I	CCWWGG	2 cut(s)	148, 906
Eco24I	GRGCYC	2 cut(s)	301, 884
Eco57I	CTGAAG	2 cut(s)	285, 1007
Eco81I	CCTNAGG	1 cut(s)	692
Eco88I	CYCGRG	1 cut(s)	1119
EcoO109I	RGGNCCY	2 cut(s)	880, 881
EcoRI	GAATTC	1 cut(s)	911
EcoRII	CCWGG	1 cut(s)	442
EcoT14I	CCWWGG	2 cut(s)	148, 906
EcoT38I	GRGCYC	2 cut(s)	301, 884
ErhI	CCWWGG	2 cut(s)	148, 906
FaeI	CATG	3 cut(s)	388, 505, 621
FalI	AAGNNNNNCTT	2 cut(s)	534, 566
FaqI	GGGAC	3 cut(s)	221, 764, 983
FatI	CATG	3 cut(s)	384, 501, 617
FauI	CCCGC	1 cut(s)	26
FauNDI	CATATG	1 cut(s)	817
Fnu4HI	GCNGC	4 cut(s)	288, 639, 651, 1072
FriOI	GRGCYC	2 cut(s)	301, 884
Fsp4HI	GCNGC	4 cut(s)	288, 639, 651, 1072
FspBI	CTAG	4 cut(s)	519, 726, 1001, 1159
GlaI	GCGC	2 cut(s)	1050, 1075
GluI	GCNGC	4 cut(s)	288, 639, 651, 1072
GsaI	CCCAGC	1 cut(s)	969
GsuI	CTGGAG	1 cut(s)	312
HaeIII	GGCC	2 cut(s)	709, 882
HapII	CCGG	1 cut(s)	1106
HgaI	GACGC	2 cut(s)	668, 980
HhaI	GCGC	2 cut(s)	1051, 1076
Hin1II	CATG	3 cut(s)	388, 505, 621
Hin6I	GCGC	2 cut(s)	1049, 1074
HinP1I	GCGC	2 cut(s)	1049, 1074
HincII	GTYRAC	1 cut(s)	74
HindII	GTYRAC	1 cut(s)	74
HinfI	GANTC	4 cut(s)	191, 752, 924, 1124
HpaII	CCGG	1 cut(s)	1106
Hpy166II	GTNNAC	4 cut(s)	74, 418, 449, 952
Hpy188I	TCNGA	2 cut(s)	86, 181
Hpy188III	TCNNGA	4 cut(s)	539, 769, 1025, 1121
Hpy8I	GTNNAC	4 cut(s)	74, 418, 449, 952
HpyAV	CCTTC	6 cut(s)	565, 720, 725, 797, 931, 1151
HpyCH4III	ACNGT	3 cut(s)	82, 705, 1084
HpyCH4IV	ACGT	1 cut(s)	493
HpyCH4V	TGCA	9 cut(s)	95, 164, 275, 314, 449, 621, 638, 653, 894
HpyF3I	CTNAG	4 cut(s)	116, 692, 876, 1040
HpySE526I	ACGT	1 cut(s)	493
Hsp92II	CATG	3 cut(s)	388, 505, 621
HspAI	GCGC	2 cut(s)	1049, 1074
Kzo9I	GATC	5 cut(s)	137, 439, 682, 811, 1009
LguI	GCTCTTC	1 cut(s)	12
LmnI	GCTCC	2 cut(s)	296, 1056
Lsp1109I	GCAGC	3 cut(s)	274, 637, 650
LweI	GCATC	1 cut(s)	1036
MaeI	CTAG	4 cut(s)	519, 726, 1001, 1159
MaeII	ACGT	1 cut(s)	493
MaeIII	GTNAC	3 cut(s)	469, 512, 871
MalI	GATC	5 cut(s)	139, 441, 684, 813, 1011
MboI	GATC	5 cut(s)	137, 439, 682, 811, 1009
MboII	GAAGA	6 cut(s)	114, 380, 791, 794, 821, 933
MflI	RGATCY	1 cut(s)	811
MhlI	GDGCHC	3 cut(s)	301, 451, 884
MluCI	AATT	7 cut(s)	55, 374, 564, 735, 839, 860, 911
MlyI	GAGTC	2 cut(s)	746, 933
MseI	TTAA	3 cut(s)	279, 563, 858
MspI	CCGG	1 cut(s)	1106
MspR9I	CCNGG	1 cut(s)	444
MvaI	CCWGG	1 cut(s)	444
NdeI	CATATG	1 cut(s)	817
NdeII	GATC	5 cut(s)	137, 439, 682, 811, 1009
NheI	GCTAGC	1 cut(s)	1000
NlaIII	CATG	3 cut(s)	388, 505, 621
NlaIV	GGNNCC	3 cut(s)	298, 615, 882
NmuCI	GTSAC	1 cut(s)	871
NspI	RCATGY	1 cut(s)	388
NspV	TTCGAA	1 cut(s)	835
PaeR7I	CTCGAG	1 cut(s)	1119
PaqCI	CACCTGC	1 cut(s)	280
PciSI	GCTCTTC	1 cut(s)	12
PfeI	GAWTC	2 cut(s)	191, 1124
PkrI	GCNGC	4 cut(s)	289, 640, 652, 1073
PleI	GAGTC	2 cut(s)	746, 932
PpsI	GAGTC	2 cut(s)	746, 932
Psp6I	CCWGG	1 cut(s)	442
PspFI	CCCAGC	1 cut(s)	965
PspGI	CCWGG	1 cut(s)	442
PspN4I	GGNNCC	3 cut(s)	298, 615, 882
PspOMI	GGGCCC	1 cut(s)	880
PspPI	GGNCC	2 cut(s)	880, 881
PsuI	RGATCY	1 cut(s)	811
RsaI	GTAC	2 cut(s)	845, 899
RsaNI	GTAC	2 cut(s)	844, 898
SapI	GCTCTTC	1 cut(s)	12
SaqAI	TTAA	3 cut(s)	279, 563, 858
SatI	GCNGC	4 cut(s)	288, 639, 651, 1072
Sau3AI	GATC	5 cut(s)	137, 439, 682, 811, 1009
Sau96I	GGNCC	2 cut(s)	880, 881
SchI	GAGTC	2 cut(s)	746, 933
ScrFI	CCNGG	1 cut(s)	444
SduI	GDGCHC	3 cut(s)	301, 451, 884
SfaNI	GCATC	1 cut(s)	1036
SfcI	CTRYAG	1 cut(s)	1152
Sfr274I	CTCGAG	1 cut(s)	1119
SfuI	TTCGAA	1 cut(s)	835
SlaI	CTCGAG	1 cut(s)	1119
SmlI	CTYRAG	2 cut(s)	1119, 1145
SmoI	CTYRAG	2 cut(s)	1119, 1145
Sse9I	AATT	7 cut(s)	55, 374, 564, 735, 839, 860, 911
SsiI	CCGC	3 cut(s)	33, 714, 1071
SspI	AATATT	2 cut(s)	582, 990
SspMI	CTAG	4 cut(s)	519, 726, 1001, 1159
StyD4I	CCNGG	1 cut(s)	442
StyI	CCWWGG	2 cut(s)	148, 906
TaaI	ACNGT	3 cut(s)	82, 705, 1084
TaiI	ACGT	1 cut(s)	496
TaqI	TCGA	3 cut(s)	140, 835, 1120
TasI	AATT	7 cut(s)	55, 374, 564, 735, 839, 860, 911
TauI	GCSGC	1 cut(s)	1074
TfiI	GAWTC	2 cut(s)	191, 1124
Tru1I	TTAA	3 cut(s)	279, 563, 858
Tru9I	TTAA	3 cut(s)	279, 563, 858
TscAI	CASTG	4 cut(s)	708, 880, 922, 964
TseFI	GTSAC	1 cut(s)	871
TseI	GCWGC	3 cut(s)	287, 638, 650
Tsp45I	GTSAC	1 cut(s)	871
TspDTI	ATGAA	5 cut(s)	246, 387, 490, 837, 950
TspGWI	ACGGA	3 cut(s)	91, 626, 815
TspRI	CASTG	4 cut(s)	708, 880, 922, 964
VneI	GTGCAC	1 cut(s)	447
XapI	RAATTY	3 cut(s)	374, 735, 911
XceI	RCATGY	1 cut(s)	388
XhoI	CTCGAG	1 cut(s)	1119
XspI	CTAG	4 cut(s)	519, 726, 1001, 1159

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.