AT1G66480

Plastid movement impaired 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015869
Physical Location & Seq
Reverse (-)
24805418 .. 24807557
2140 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66480.1

Sequence Viewer

Length: 678 bp
ATGGGAAACAGCATTACTGTGAAGAGAAAAAGAGCTAAAGTAATGAAGATCGACGGCGAAACTTTCCGAATTAAAACTCCGGTGACGGCGAGAGAAGTCACCGCGGATTATCCCGGTTACGTTTTGTTAGATTCTCAAGCCGTTAAACACTTTGGAGTTCGCTCTAAGCCGCTTGAACCGAACCAAACCTTGAAGCCGAAAAAGACTTATTTTCTCGTCGAGCTTCCTAAGCTTCCACCGGAGACGACGGCGGTGGATACAGAAAATAAGCTACCGTACCGGAGAGTGATGTCTGGTATCCACGTTGGCGCAAAGGAGCGGTTAGATATGTTGATGTTGTCTCGGAGAACGGTTTCTGACGTCACAATCGGAAGATCTGACGGTGGAGATGGGTTTGGGCCTGAGCTTGGGCCTGGACATACGAGTGTGAGGTTGAGGTTGCCTCGTTCTCAGATCACGAAGCTTATGGAAGAGAATAATAATGATGCTTCGGCCATAGCTGAGAAGATTTTAGGTATCTATATGGAGAGGTCTGGCGAGCTTGGCGGCGGTAGAGGTGGTGTTGACGGCCGCCGGGAACTTGGAAGTGGGGAGATTAAAGCGCGTGAGAAGCAGGTGAGTTTCGCCGGAGAAGGTGGACGGGAGCTTCCTGTTCTATGGAGTAGAAGTGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

225

Amino Acids

24.73

Weight (kDa)

9.87

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 97 7.7e-20 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015869)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66480 AT5G37840
malus_domestica MD09G1261400.v1.1
prunus_persica Prupe.3G158800_v2.0.a1
pyrus_communis pycom09g17580
rosa_chinensis RchiOBHm_Chr3g0493541
rosa_laevigata RLG00000022707
rosa_multiflora Rmu_sc0002343.1_g000048
rosa_roxburghii Rroxscaffold_6G00391510
rosa_rugosa Rorug03G0261900
rosa_samantha Rh3AG310900 Rh3BG346900 Rh3CG344800 Rh3DG346200
rosa_wichuraiana Rw3G027330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 604
AatII GACGTC 1 cut(s) 363
Acc36I ACCTGC 1 cut(s) 604
AccBSI CCGCTC 1 cut(s) 319
AccII CGCG 2 cut(s) 104, 604
AciI CCGC 8 cut(s) 102, 104, 170, 251, 319, 546, 549, 571
AcoI YGGCCR 2 cut(s) 492, 568
AcyI GRCGYC 1 cut(s) 360
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 1 cut(s) 407
AgsI TTSAA 2 cut(s) 176, 193
AjnI CCWGG 1 cut(s) 412
AluBI AGCT 9 cut(s) 35, 223, 232, 271, 406, 463, 500, 541, 646
AluI AGCT 9 cut(s) 35, 223, 232, 271, 406, 463, 500, 541, 646
Alw26I GTCTC 2 cut(s) 236, 345
AoxI GGCC 4 cut(s) 398, 410, 492, 568
Asp700I GAANNNNTTC 1 cut(s) 352
AspLEI GCGC 2 cut(s) 311, 604
AspS9I GGNCC 2 cut(s) 398, 410
AsuC2I CCSGG 2 cut(s) 114, 575
AsuHPI GGTGA 3 cut(s) 91, 94, 628
BccI CCATC 1 cut(s) 383
BceAI ACGGC 5 cut(s) 70, 102, 125, 264, 583
BciT130I CCWGG 1 cut(s) 414
BciVI GTATCC 2 cut(s) 250, 308
BcnI CCSGG 2 cut(s) 114, 575
BcoDI GTCTC 2 cut(s) 236, 345
BfuAI ACCTGC 1 cut(s) 604
BfuI GTATCC 2 cut(s) 250, 308
BglII AGATCT 1 cut(s) 374
BisI GCNGC 3 cut(s) 170, 547, 571
BlsI GCNGC 3 cut(s) 171, 548, 572
Bme1390I CCNGG 3 cut(s) 114, 414, 575
BmgT120I GGNCC 2 cut(s) 398, 410
BmrFI CCNGG 3 cut(s) 114, 414, 575
BmsI GCATC 1 cut(s) 475
BplI GAGNNNNNCTC 2 cut(s) 427, 459
Bpu10I CCTNAGC 2 cut(s) 228, 402
BpuEI CTTGAG 1 cut(s) 120
BpuMI CCSGG 2 cut(s) 114, 575
BsaHI GRCGYC 1 cut(s) 360
BsaJI CCNNGG 1 cut(s) 102
BsaWI WCCGGW 3 cut(s) 79, 238, 279
BsaXI ACNNNNNCTCC 1 cut(s) 652
Bsc4I CCNNNNNNNGG 1 cut(s) 407
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 102
BseLI CCNNNNNNNGG 1 cut(s) 407
BseMII CTCAG 3 cut(s) 393, 464, 492
BseX3I CGGCCG 1 cut(s) 568
Bsh1236I CGCG 2 cut(s) 104, 604
Bsh1285I CGRYCG 1 cut(s) 571
BshFI GGCC 4 cut(s) 400, 412, 494, 570
BsiEI CGRYCG 1 cut(s) 571
BsiSI CCGG 6 cut(s) 80, 114, 239, 280, 574, 627
BslI CCNNNNNNNGG 1 cut(s) 407
BsmAI GTCTC 2 cut(s) 236, 345
BsmBI CGTCTC 1 cut(s) 236
BsnI GGCC 4 cut(s) 400, 412, 494, 570
Bsp143I GATC 3 cut(s) 48, 374, 453
BspACI CCGC 8 cut(s) 102, 104, 170, 251, 319, 546, 549, 571
BspANI GGCC 4 cut(s) 400, 412, 494, 570
BspCNI CTCAG 3 cut(s) 394, 463, 493
BspFNI CGCG 2 cut(s) 104, 604
BspMI ACCTGC 1 cut(s) 604
BsrBI CCGCTC 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 3 cut(s) 48, 374, 453
BssNI GRCGYC 1 cut(s) 360
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 4 cut(s) 19, 276, 352, 383
Bst6I CTCTTC 2 cut(s) 17, 465
BstACI GRCGYC 1 cut(s) 360
BstC8I GCNNGC 1 cut(s) 539
BstDEI CTNAG 5 cut(s) 165, 228, 402, 450, 501
BstDSI CCRYGG 1 cut(s) 102
BstFNI CGCG 2 cut(s) 104, 604
BstHHI GCGC 2 cut(s) 311, 604
BstKTI GATC 3 cut(s) 51, 377, 456
BstMAI GTCTC 2 cut(s) 236, 345
BstMBI GATC 3 cut(s) 48, 374, 453
BstMCI CGRYCG 1 cut(s) 571
BstMWI GCNNNNNNNGC 3 cut(s) 229, 543, 610
BstNI CCWGG 1 cut(s) 414
BstSCI CCNGG 3 cut(s) 112, 412, 573
BstUI CGCG 2 cut(s) 104, 604
BstX2I RGATCY 1 cut(s) 374
BstYI RGATCY 1 cut(s) 374
BstZI CGGCCG 1 cut(s) 568
BsuI GTATCC 2 cut(s) 250, 308
BsuRI GGCC 4 cut(s) 400, 412, 494, 570
BtgI CCRYGG 1 cut(s) 102
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 1 cut(s) 539
CfoI GCGC 2 cut(s) 311, 604
Cfr13I GGNCC 2 cut(s) 398, 410
Cfr42I CCGCGG 1 cut(s) 105
Csp6I GTAC 1 cut(s) 277
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 5 cut(s) 165, 228, 402, 450, 501
DpnI GATC 3 cut(s) 50, 376, 455
DpnII GATC 3 cut(s) 48, 374, 453
EaeI YGGCCR 2 cut(s) 492, 568
EagI CGGCCG 1 cut(s) 568
Eam1104I CTCTTC 2 cut(s) 17, 465
EarI CTCTTC 2 cut(s) 17, 465
EclXI CGGCCG 1 cut(s) 568
Eco52I CGGCCG 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 412
Esp3I CGTCTC 1 cut(s) 236
FaiI YATR 7 cut(s) 329, 420, 467, 497, 522, 524, 658
Fnu4HI GCNGC 3 cut(s) 170, 547, 571
Fsp4HI GCNGC 3 cut(s) 170, 547, 571
GlaI GCGC 2 cut(s) 310, 603
GluI GCNGC 3 cut(s) 170, 547, 571
HaeIII GGCC 4 cut(s) 400, 412, 494, 570
HapII CCGG 6 cut(s) 80, 114, 239, 280, 574, 627
HhaI GCGC 2 cut(s) 311, 604
Hin1I GRCGYC 1 cut(s) 360
Hin6I GCGC 2 cut(s) 309, 602
HinP1I GCGC 2 cut(s) 309, 602
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 2 cut(s) 230, 461
HinfI GANTC 1 cut(s) 131
HpaII CCGG 6 cut(s) 80, 114, 239, 280, 574, 627
HphI GGTGA 3 cut(s) 91, 94, 628
Hpy166II GTNNAC 2 cut(s) 565, 638
Hpy188I TCNGA 6 cut(s) 68, 345, 358, 371, 379, 453
Hpy188III TCNNGA 1 cut(s) 457
Hpy8I GTNNAC 2 cut(s) 565, 638
Hpy99I CGWCG 3 cut(s) 56, 221, 250
HpyAV CCTTC 1 cut(s) 626
HpyCH4III ACNGT 4 cut(s) 19, 276, 352, 383
HpyCH4IV ACGT 3 cut(s) 120, 303, 360
HpyF10VI GCNNNNNNNGC 3 cut(s) 229, 543, 610
HpyF3I CTNAG 5 cut(s) 165, 228, 402, 450, 501
HpySE526I ACGT 3 cut(s) 120, 303, 360
Hsp92I GRCGYC 1 cut(s) 360
HspAI GCGC 2 cut(s) 309, 602
KspI CCGCGG 1 cut(s) 105
Kzo9I GATC 3 cut(s) 48, 374, 453
LmnI GCTCC 2 cut(s) 316, 643
LweI GCATC 1 cut(s) 475
MaeII ACGT 3 cut(s) 120, 303, 360
MaeIII GTNAC 4 cut(s) 82, 97, 116, 361
MalI GATC 3 cut(s) 50, 376, 455
MbiI CCGCTC 1 cut(s) 319
MboI GATC 3 cut(s) 48, 374, 453
MboII GAAGA 5 cut(s) 34, 58, 384, 482, 517
MflI RGATCY 1 cut(s) 374
MluCI AATT 1 cut(s) 69
MnlI CCTC 5 cut(s) 423, 429, 453, 522, 548
MroXI GAANNNNTTC 1 cut(s) 352
MseI TTAA 3 cut(s) 72, 144, 597
MslI CAYNNNNRTG 2 cut(s) 17, 423
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 6 cut(s) 80, 114, 239, 280, 574, 627
MspR9I CCNGG 3 cut(s) 114, 414, 575
MvaI CCWGG 1 cut(s) 414
MvnI CGCG 2 cut(s) 104, 604
MwoI GCNNNNNNNGC 3 cut(s) 229, 543, 610
NciI CCSGG 2 cut(s) 114, 575
NdeII GATC 3 cut(s) 48, 374, 453
NmuCI GTSAC 3 cut(s) 82, 97, 361
PaqCI CACCTGC 1 cut(s) 604
PdmI GAANNNNTTC 1 cut(s) 352
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 3 cut(s) 171, 548, 572
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspPI GGNCC 2 cut(s) 398, 410
PsuI RGATCY 1 cut(s) 374
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
RseI CAYNNNNRTG 2 cut(s) 17, 423
SacII CCGCGG 1 cut(s) 105
SaqAI TTAA 3 cut(s) 72, 144, 597
SatI GCNGC 3 cut(s) 170, 547, 571
Sau3AI GATC 3 cut(s) 48, 374, 453
Sau96I GGNCC 2 cut(s) 398, 410
ScrFI CCNGG 3 cut(s) 114, 414, 575
SfaNI GCATC 1 cut(s) 475
Sfr303I CCGCGG 1 cut(s) 105
SgrBI CCGCGG 1 cut(s) 105
SmiMI CAYNNNNRTG 2 cut(s) 17, 423
SmlI CTYRAG 1 cut(s) 135
SmoI CTYRAG 1 cut(s) 135
Sse9I AATT 1 cut(s) 69
SsiI CCGC 8 cut(s) 102, 104, 170, 251, 319, 546, 549, 571
StyD4I CCNGG 3 cut(s) 112, 412, 573
TaaI ACNGT 4 cut(s) 19, 276, 352, 383
TaiI ACGT 3 cut(s) 123, 306, 363
TaqI TCGA 2 cut(s) 51, 219
TasI AATT 1 cut(s) 69
TauI GCSGC 3 cut(s) 172, 549, 573
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 3 cut(s) 72, 144, 597
Tru9I TTAA 3 cut(s) 72, 144, 597
TseFI GTSAC 3 cut(s) 82, 97, 361
Tsp45I GTSAC 3 cut(s) 82, 97, 361
TspDTI ATGAA 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 352
ZraI GACGTC 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.