Rh3AG310900

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0015869
Physical Location & Seq
Forward (+)
39152600 .. 39155265
2666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG310900.1

Sequence Viewer

Length: 672 bp
ATGGGAAACACAATTGGAGGAAAGAGCAGGAAAGCAAAGGTGATGAAGATAAGTGGAGAAACGTTCAAGGTAAAGACTCCCATACGAGCTGGGGATGTAGTGAAGGAGCACCCAGGTCATATGCTATTGAATTCTGAAGCTGTTAAACATTTTGGTATGAGAGCTAAGCCATTAGAAGCGCAACAAGAGCTCAAGCCCAGGAGTATATATTTTCTGGTAGAATTGCCCAATTTTGATTATATGAATTCGGAAGAAGAAGCAGGAAGAAGAAGTAATAGCAGGGTGGCGAGAAGGGTGAGATCGTCGGCCAATATTCAAGTGAGCGCCAAGGAGAGGCTCGAGTGCTTGATGCTGTCTCGACGCTCTGCTTCCGACCTTACGAGTCTTGTGAGAGCAGCACCACCATCTTTAGGAGGAGCAGGAGGAGGAGGAGGAGCTCCGGTGAGGATGAAGATGAGGCTGCCGAGGGCGCAGGTGGTCAAATTGATGGAGGAGAGTAAGGATGAGATTGAAGTCGCCGACAAAATCATTCAGCTCTATATGGCCACTGCCGGCACCGTCGGCGATGACAGCAACACGGACAAAAACTTCACTTTCAAGGCGCATCAGAAGCGAGTGAGTTTTGTCCCCGAGAAGAAAGGAGAAATTCAATTAGATGTAGCTCCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

223

Amino Acids

24.55

Weight (kDa)

9.88

Isoelectric Point (pI)

54.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 166 3.5e-21 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015869)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66480 AT5G37840
malus_domestica MD09G1261400.v1.1
prunus_persica Prupe.3G158800_v2.0.a1
pyrus_communis pycom09g17580
rosa_chinensis RchiOBHm_Chr3g0493541
rosa_laevigata RLG00000022707
rosa_multiflora Rmu_sc0002343.1_g000048
rosa_roxburghii Rroxscaffold_6G00391510
rosa_rugosa Rorug03G0261900
rosa_samantha Rh3AG310900 Rh3BG346900 Rh3CG344800 Rh3DG346200
rosa_wichuraiana Rw3G027330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 463
Acc36I ACCTGC 1 cut(s) 463
AccB1I GGYRCC 1 cut(s) 554
AclI AACGTT 1 cut(s) 62
AcoI YGGCCR 2 cut(s) 306, 543
AcsI RAATTY 3 cut(s) 130, 244, 645
AcuI CTGAAG 1 cut(s) 156
AfiI CCNNNNNNNGG 2 cut(s) 333, 410
AgsI TTSAA 6 cut(s) 67, 130, 317, 512, 598, 650
AjnI CCWGG 2 cut(s) 112, 197
AluBI AGCT 7 cut(s) 89, 140, 164, 190, 437, 535, 662
AluI AGCT 7 cut(s) 89, 140, 164, 190, 437, 535, 662
Alw21I GWGCWC 3 cut(s) 111, 192, 439
Alw26I GTCTC 1 cut(s) 360
Ama87I CYCGRG 2 cut(s) 338, 629
AoxI GGCC 2 cut(s) 306, 543
ApeKI GCWGC 2 cut(s) 395, 460
ApoI RAATTY 3 cut(s) 130, 244, 645
AspLEI GCGC 4 cut(s) 181, 326, 472, 604
AsuHPI GGTGA 3 cut(s) 52, 307, 454
AvaI CYCGRG 2 cut(s) 338, 629
BalI TGGCCA 1 cut(s) 545
BanI GGYRCC 1 cut(s) 554
BanII GRGCYC 2 cut(s) 192, 439
Bbv12I GWGCWC 3 cut(s) 111, 192, 439
BbvI GCAGC 2 cut(s) 407, 447
BccI CCATC 2 cut(s) 412, 481
BciT130I CCWGG 2 cut(s) 114, 199
BcoDI GTCTC 1 cut(s) 360
BfoI RGCGCY 1 cut(s) 327
BfuAI ACCTGC 1 cut(s) 463
BisI GCNGC 2 cut(s) 396, 461
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 2 cut(s) 397, 462
Bme1390I CCNGG 2 cut(s) 114, 199
BmeT110I CYCGRG 2 cut(s) 338, 629
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 2 cut(s) 114, 199
BmsI GCATC 2 cut(s) 339, 613
Bpu1102I GCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 176
BsaJI CCNNGG 4 cut(s) 112, 197, 327, 464
BsaWI WCCGGW 1 cut(s) 439
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bsc4I CCNNNNNNNGG 2 cut(s) 333, 410
Bse118I RCCGGY 1 cut(s) 551
BseBI CCWGG 2 cut(s) 114, 199
BseDI CCNNGG 4 cut(s) 112, 197, 327, 464
BseGI GGATG 3 cut(s) 100, 453, 508
BseLI CCNNNNNNNGG 2 cut(s) 333, 410
BseRI GAGGAG 7 cut(s) 429, 438, 441, 444, 447, 506, 654
BseXI GCAGC 2 cut(s) 407, 447
BseYI CCCAGC 1 cut(s) 89
BshFI GGCC 2 cut(s) 308, 545
BshNI GGYRCC 1 cut(s) 554
BsiHKAI GWGCWC 3 cut(s) 111, 192, 439
BsiHKCI CYCGRG 2 cut(s) 338, 629
BsiSI CCGG 2 cut(s) 440, 552
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 2 cut(s) 333, 410
BsmAI GTCTC 1 cut(s) 360
BsmFI GGGAC 1 cut(s) 611
BsnI GGCC 2 cut(s) 308, 545
BsoBI CYCGRG 2 cut(s) 338, 629
Bsp1286I GDGCHC 3 cut(s) 111, 192, 439
Bsp143I GATC 1 cut(s) 299
Bsp1720I GCTNAGC 1 cut(s) 165
BspANI GGCC 2 cut(s) 308, 545
BspLI GGNNCC 1 cut(s) 556
BspMI ACCTGC 1 cut(s) 463
BspT107I GGYRCC 1 cut(s) 554
BsrFI RCCGGY 1 cut(s) 551
BssAI RCCGGY 1 cut(s) 551
BssECI CCNNGG 4 cut(s) 112, 197, 327, 464
BssMI GATC 1 cut(s) 299
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 114, 199
Bst4CI ACNGT 1 cut(s) 559
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 3 cut(s) 100, 453, 508
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 4 cut(s) 181, 326, 472, 604
BstKTI GATC 1 cut(s) 302
BstMAI GTCTC 1 cut(s) 360
BstMBI GATC 1 cut(s) 299
BstMWI GCNNNNNNNGC 5 cut(s) 187, 469, 561, 570, 610
BstNI CCWGG 2 cut(s) 114, 199
BstSCI CCNGG 2 cut(s) 112, 197
BstV1I GCAGC 2 cut(s) 407, 447
BsuRI GGCC 2 cut(s) 308, 545
BtgZI GCGATG 1 cut(s) 579
BtsCI GGATG 3 cut(s) 100, 453, 508
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 1 cut(s) 546
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 1 cut(s) 553
CfoI GCGC 4 cut(s) 181, 326, 472, 604
Cfr10I RCCGGY 1 cut(s) 551
CseI GACGC 1 cut(s) 369
DdeI CTNAG 1 cut(s) 165
DpnI GATC 1 cut(s) 301
DpnII GATC 1 cut(s) 299
EaeI YGGCCR 2 cut(s) 306, 543
Ecl136II GAGCTC 2 cut(s) 190, 437
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 2 cut(s) 192, 439
Eco53kI GAGCTC 2 cut(s) 190, 437
Eco57I CTGAAG 1 cut(s) 156
Eco88I CYCGRG 2 cut(s) 338, 629
EcoICRI GAGCTC 2 cut(s) 190, 437
EcoRI GAATTC 2 cut(s) 130, 244
EcoRII CCWGG 2 cut(s) 112, 197
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 2 cut(s) 192, 439
ErhI CCWWGG 1 cut(s) 327
FaqI GGGAC 1 cut(s) 611
FauNDI CATATG 1 cut(s) 120
Fnu4HI GCNGC 2 cut(s) 396, 461
FokI GGATG 3 cut(s) 107, 460, 515
FriOI GRGCYC 2 cut(s) 192, 439
Fsp4HI GCNGC 2 cut(s) 396, 461
GlaI GCGC 4 cut(s) 180, 325, 471, 603
GluI GCNGC 2 cut(s) 396, 461
GsaI CCCAGC 1 cut(s) 93
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 2 cut(s) 308, 545
HapII CCGG 2 cut(s) 440, 552
HgaI GACGC 1 cut(s) 369
HhaI GCGC 4 cut(s) 181, 326, 472, 604
Hin6I GCGC 4 cut(s) 179, 324, 470, 602
HinP1I GCGC 4 cut(s) 179, 324, 470, 602
HinfI GANTC 2 cut(s) 76, 382
HpaII CCGG 2 cut(s) 440, 552
HphI GGTGA 3 cut(s) 52, 307, 454
Hpy188I TCNGA 4 cut(s) 136, 250, 373, 609
Hpy188III TCNNGA 1 cut(s) 357
Hpy99I CGWCG 3 cut(s) 307, 363, 563
HpyAV CCTTC 2 cut(s) 97, 285
HpyCH4III ACNGT 1 cut(s) 559
HpyCH4IV ACGT 1 cut(s) 62
HpyF10VI GCNNNNNNNGC 5 cut(s) 187, 469, 561, 570, 610
HpyF3I CTNAG 1 cut(s) 165
HpySE526I ACGT 1 cut(s) 62
HspAI GCGC 4 cut(s) 179, 324, 470, 602
KroI GCCGGC 1 cut(s) 551
KroNI GCCGGC 1 cut(s) 553
Kzo9I GATC 1 cut(s) 299
LmnI GCTCC 5 cut(s) 106, 416, 434, 442, 667
Lsp1109I GCAGC 2 cut(s) 407, 447
LweI GCATC 2 cut(s) 339, 613
MaeII ACGT 1 cut(s) 62
MalI GATC 1 cut(s) 301
MboI GATC 1 cut(s) 299
MboII GAAGA 7 cut(s) 58, 263, 266, 276, 279, 463, 646
MfeI CAATTG 1 cut(s) 12
MhlI GDGCHC 3 cut(s) 111, 192, 439
MlsI TGGCCA 1 cut(s) 545
MluCI AATT 8 cut(s) 12, 130, 221, 229, 244, 482, 645, 650
MluNI TGGCCA 1 cut(s) 545
MlyI GAGTC 2 cut(s) 70, 391
MmeI TCCRAC 1 cut(s) 396
Mox20I TGGCCA 1 cut(s) 545
MroNI GCCGGC 1 cut(s) 551
MscI TGGCCA 1 cut(s) 545
MseI TTAA 1 cut(s) 144
Msp20I TGGCCA 1 cut(s) 545
MspI CCGG 2 cut(s) 440, 552
MspR9I CCNGG 2 cut(s) 114, 199
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 2 cut(s) 114, 199
MwoI GCNNNNNNNGC 5 cut(s) 187, 469, 561, 570, 610
NaeI GCCGGC 1 cut(s) 553
NdeI CATATG 1 cut(s) 120
NdeII GATC 1 cut(s) 299
NgoMIV GCCGGC 1 cut(s) 551
NlaIV GGNNCC 1 cut(s) 556
NmeAIII GCCGAG 1 cut(s) 489
PaeR7I CTCGAG 1 cut(s) 338
PaqCI CACCTGC 1 cut(s) 463
PdiI GCCGGC 1 cut(s) 553
PkrI GCNGC 2 cut(s) 397, 462
PleI GAGTC 2 cut(s) 70, 390
PpsI GAGTC 2 cut(s) 70, 390
Psp124BI GAGCTC 2 cut(s) 192, 439
Psp1406I AACGTT 1 cut(s) 62
Psp6I CCWGG 2 cut(s) 112, 197
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 2 cut(s) 112, 197
PspN4I GGNNCC 1 cut(s) 556
PspXI VCTCGAGB 1 cut(s) 338
SacI GAGCTC 2 cut(s) 192, 439
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 2 cut(s) 396, 461
Sau3AI GATC 1 cut(s) 299
SchI GAGTC 2 cut(s) 70, 391
ScrFI CCNGG 2 cut(s) 114, 199
SduI GDGCHC 3 cut(s) 111, 192, 439
SfaNI GCATC 2 cut(s) 339, 613
Sfr274I CTCGAG 1 cut(s) 338
SlaI CTCGAG 1 cut(s) 338
SmlI CTYRAG 2 cut(s) 191, 338
SmoI CTYRAG 2 cut(s) 191, 338
Sse9I AATT 8 cut(s) 12, 130, 221, 229, 244, 482, 645, 650
SspI AATATT 1 cut(s) 313
SstI GAGCTC 2 cut(s) 192, 439
StyD4I CCNGG 2 cut(s) 112, 197
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 559
TaiI ACGT 1 cut(s) 65
TaqI TCGA 2 cut(s) 339, 358
TasI AATT 8 cut(s) 12, 130, 221, 229, 244, 482, 645, 650
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 1 cut(s) 553
TseI GCWGC 2 cut(s) 395, 460
TspDTI ATGAA 3 cut(s) 59, 257, 464
TspGWI ACGGA 1 cut(s) 593
TspRI CASTG 1 cut(s) 553
XapI RAATTY 3 cut(s) 130, 244, 645
XhoI CTCGAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.