Rw3G027330

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0015869
Physical Location & Seq
Forward (+)
38555391 .. 38557630
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G027330.1

Sequence Viewer

Length: 663 bp
ATGGGAAACACAATTGGAGGAAAAAGCAGGAAAGCAAAGGTGATGAAGATAAGTGGAGAAACGTTCAAGGTAAAGACTCCCATACGAGCTGGGGATCTAGTGAAGGAGCACCCAGGTCATATGCTATTGAATTCTGAAGCTGTTAAACATTTTGGTATGAGAGCTAAGCCATTAGAAGCGCAACAAGAGCTCAAGCCCAGGAGTATATATTTTCTGGTAGAATTGCCCAATTTTGATTATATGAATTCGGAAGAAGAAGCAGGAAGAAGAAGTAATAGCAGGGTGGCGAGAAGGGTGAGATCGTCGGCCAATATTCAAGTGAGCGCCAAGGAGAGGCTCGAGTGCTTGATGCTGTCTCGACGCTCTGCTTCCGACGTTACGAGTCTTGTGAGAGCAGCACCACCATCTTTAGGAGGAGCAGGAGGAGCTCCGGTGAGGATGAAGATGAGGCTGCCGAGGGCGCAGGTGGTCAAATTGATGGAGGAGAGTAAGGATGAGATTGAAGTCACCGACAAAATCATTCAGCTCTATATGGCCAATGCCGGCACCGTCGGCAATAACAGCAACACGGACAAAAACTTCACTTTCAAGGCGCGTCAGAAGCGAGTGAGTTTTGTCCCCGAGAAGAAAGGAGAAATTCAATTAGATGTAGCTCCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

220

Amino Acids

24.44

Weight (kDa)

10.1

Isoelectric Point (pI)

52.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 166 5.9e-21 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015869)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66480 AT5G37840
malus_domestica MD09G1261400.v1.1
prunus_persica Prupe.3G158800_v2.0.a1
pyrus_communis pycom09g17580
rosa_chinensis RchiOBHm_Chr3g0493541
rosa_laevigata RLG00000022707
rosa_multiflora Rmu_sc0002343.1_g000048
rosa_roxburghii Rroxscaffold_6G00391510
rosa_rugosa Rorug03G0261900
rosa_samantha Rh3AG310900 Rh3BG346900 Rh3CG344800 Rh3DG346200
rosa_wichuraiana Rw3G027330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 454
Acc36I ACCTGC 1 cut(s) 454
AccB1I GGYRCC 1 cut(s) 545
AccII CGCG 1 cut(s) 595
AclI AACGTT 1 cut(s) 62
AclWI GGATC 1 cut(s) 102
AcoI YGGCCR 2 cut(s) 306, 534
AcsI RAATTY 3 cut(s) 130, 244, 636
AcuI CTGAAG 1 cut(s) 156
AfiI CCNNNNNNNGG 2 cut(s) 333, 410
AgsI TTSAA 6 cut(s) 67, 130, 317, 503, 589, 641
AjnI CCWGG 2 cut(s) 112, 197
AluBI AGCT 7 cut(s) 89, 140, 164, 190, 428, 526, 653
AluI AGCT 7 cut(s) 89, 140, 164, 190, 428, 526, 653
Alw21I GWGCWC 3 cut(s) 111, 192, 430
Alw26I GTCTC 1 cut(s) 360
AlwI GGATC 1 cut(s) 102
Ama87I CYCGRG 2 cut(s) 338, 620
AoxI GGCC 2 cut(s) 306, 534
ApeKI GCWGC 2 cut(s) 395, 451
ApoI RAATTY 3 cut(s) 130, 244, 636
AspLEI GCGC 4 cut(s) 181, 326, 463, 595
AsuHPI GGTGA 4 cut(s) 52, 307, 445, 499
AvaI CYCGRG 2 cut(s) 338, 620
BalI TGGCCA 1 cut(s) 536
BanI GGYRCC 1 cut(s) 545
BanII GRGCYC 2 cut(s) 192, 430
Bbv12I GWGCWC 3 cut(s) 111, 192, 430
BbvI GCAGC 2 cut(s) 407, 438
BccI CCATC 2 cut(s) 412, 472
BciT130I CCWGG 2 cut(s) 114, 199
BcoDI GTCTC 1 cut(s) 360
BfaI CTAG 1 cut(s) 98
BfoI RGCGCY 1 cut(s) 327
BfuAI ACCTGC 1 cut(s) 454
BisI GCNGC 2 cut(s) 396, 452
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 2 cut(s) 397, 453
Bme1390I CCNGG 2 cut(s) 114, 199
BmeT110I CYCGRG 2 cut(s) 338, 620
BmiI GGNNCC 1 cut(s) 547
BmrFI CCNGG 2 cut(s) 114, 199
BmsI GCATC 1 cut(s) 339
Bpu1102I GCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 176
BsaJI CCNNGG 4 cut(s) 112, 197, 327, 455
BsaWI WCCGGW 1 cut(s) 430
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 2 cut(s) 333, 410
Bse118I RCCGGY 1 cut(s) 542
BseBI CCWGG 2 cut(s) 114, 199
BseDI CCNNGG 4 cut(s) 112, 197, 327, 455
BseGI GGATG 2 cut(s) 444, 499
BseLI CCNNNNNNNGG 2 cut(s) 333, 410
BseRI GAGGAG 4 cut(s) 429, 438, 497, 645
BseXI GCAGC 2 cut(s) 407, 438
BseYI CCCAGC 1 cut(s) 89
Bsh1236I CGCG 1 cut(s) 595
BshFI GGCC 2 cut(s) 308, 536
BshNI GGYRCC 1 cut(s) 545
BsiHKAI GWGCWC 3 cut(s) 111, 192, 430
BsiHKCI CYCGRG 2 cut(s) 338, 620
BsiSI CCGG 2 cut(s) 431, 543
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 2 cut(s) 333, 410
BsmAI GTCTC 1 cut(s) 360
BsmFI GGGAC 1 cut(s) 602
BsnI GGCC 2 cut(s) 308, 536
BsoBI CYCGRG 2 cut(s) 338, 620
Bsp1286I GDGCHC 3 cut(s) 111, 192, 430
Bsp143I GATC 2 cut(s) 94, 299
Bsp1720I GCTNAGC 1 cut(s) 165
BspANI GGCC 2 cut(s) 308, 536
BspFNI CGCG 1 cut(s) 595
BspLI GGNNCC 1 cut(s) 547
BspMI ACCTGC 1 cut(s) 454
BspPI GGATC 1 cut(s) 102
BspT107I GGYRCC 1 cut(s) 545
BsrFI RCCGGY 1 cut(s) 542
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 4 cut(s) 112, 197, 327, 455
BssMI GATC 2 cut(s) 94, 299
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 114, 199
Bst4CI ACNGT 1 cut(s) 550
BstC8I GCNNGC 1 cut(s) 544
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 2 cut(s) 444, 499
BstFNI CGCG 1 cut(s) 595
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 4 cut(s) 181, 326, 463, 595
BstKTI GATC 2 cut(s) 97, 302
BstMAI GTCTC 1 cut(s) 360
BstMBI GATC 2 cut(s) 94, 299
BstMWI GCNNNNNNNGC 6 cut(s) 187, 425, 460, 552, 561, 601
BstNI CCWGG 2 cut(s) 114, 199
BstSCI CCNGG 2 cut(s) 112, 197
BstUI CGCG 1 cut(s) 595
BstV1I GCAGC 2 cut(s) 407, 438
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BsuRI GGCC 2 cut(s) 308, 536
BtsCI GGATG 2 cut(s) 444, 499
BveI ACCTGC 1 cut(s) 454
Cac8I GCNNGC 1 cut(s) 544
CfoI GCGC 4 cut(s) 181, 326, 463, 595
Cfr10I RCCGGY 1 cut(s) 542
CseI GACGC 2 cut(s) 369, 584
DdeI CTNAG 1 cut(s) 165
DpnI GATC 2 cut(s) 96, 301
DpnII GATC 2 cut(s) 94, 299
EaeI YGGCCR 2 cut(s) 306, 534
Ecl136II GAGCTC 2 cut(s) 190, 428
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 2 cut(s) 192, 430
Eco53kI GAGCTC 2 cut(s) 190, 428
Eco57I CTGAAG 1 cut(s) 156
Eco88I CYCGRG 2 cut(s) 338, 620
EcoICRI GAGCTC 2 cut(s) 190, 428
EcoRI GAATTC 2 cut(s) 130, 244
EcoRII CCWGG 2 cut(s) 112, 197
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 2 cut(s) 192, 430
ErhI CCWWGG 1 cut(s) 327
FaqI GGGAC 1 cut(s) 602
FauNDI CATATG 1 cut(s) 120
Fnu4HI GCNGC 2 cut(s) 396, 452
FokI GGATG 2 cut(s) 451, 506
FriOI GRGCYC 2 cut(s) 192, 430
Fsp4HI GCNGC 2 cut(s) 396, 452
FspBI CTAG 1 cut(s) 98
GlaI GCGC 4 cut(s) 180, 325, 462, 594
GluI GCNGC 2 cut(s) 396, 452
GsaI CCCAGC 1 cut(s) 93
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 2 cut(s) 308, 536
HapII CCGG 2 cut(s) 431, 543
HgaI GACGC 2 cut(s) 369, 584
HhaI GCGC 4 cut(s) 181, 326, 463, 595
Hin6I GCGC 4 cut(s) 179, 324, 461, 593
HinP1I GCGC 4 cut(s) 179, 324, 461, 593
HinfI GANTC 2 cut(s) 76, 382
HpaII CCGG 2 cut(s) 431, 543
HphI GGTGA 4 cut(s) 52, 307, 445, 499
Hpy188I TCNGA 4 cut(s) 136, 250, 373, 600
Hpy188III TCNNGA 1 cut(s) 357
Hpy99I CGWCG 4 cut(s) 307, 363, 377, 554
HpyAV CCTTC 2 cut(s) 97, 285
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4IV ACGT 2 cut(s) 62, 375
HpyF10VI GCNNNNNNNGC 6 cut(s) 187, 425, 460, 552, 561, 601
HpyF3I CTNAG 1 cut(s) 165
HpySE526I ACGT 2 cut(s) 62, 375
HspAI GCGC 4 cut(s) 179, 324, 461, 593
KroI GCCGGC 1 cut(s) 542
KroNI GCCGGC 1 cut(s) 544
Kzo9I GATC 2 cut(s) 94, 299
LmnI GCTCC 5 cut(s) 106, 416, 425, 433, 658
Lsp1109I GCAGC 2 cut(s) 407, 438
LweI GCATC 1 cut(s) 339
MaeI CTAG 1 cut(s) 98
MaeII ACGT 2 cut(s) 62, 375
MaeIII GTNAC 2 cut(s) 376, 505
MalI GATC 2 cut(s) 96, 301
MboI GATC 2 cut(s) 94, 299
MboII GAAGA 7 cut(s) 58, 263, 266, 276, 279, 454, 637
MfeI CAATTG 1 cut(s) 12
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 3 cut(s) 111, 192, 430
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 8 cut(s) 12, 130, 221, 229, 244, 473, 636, 641
MluNI TGGCCA 1 cut(s) 536
MlyI GAGTC 2 cut(s) 70, 391
MmeI TCCRAC 1 cut(s) 396
MnlI CCTC 8 cut(s) 11, 327, 407, 416, 429, 441, 450, 475
Mox20I TGGCCA 1 cut(s) 536
MroNI GCCGGC 1 cut(s) 542
MscI TGGCCA 1 cut(s) 536
MseI TTAA 1 cut(s) 144
Msp20I TGGCCA 1 cut(s) 536
MspI CCGG 2 cut(s) 431, 543
MspR9I CCNGG 2 cut(s) 114, 199
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 2 cut(s) 114, 199
MvnI CGCG 1 cut(s) 595
MwoI GCNNNNNNNGC 6 cut(s) 187, 425, 460, 552, 561, 601
NaeI GCCGGC 1 cut(s) 544
NdeI CATATG 1 cut(s) 120
NdeII GATC 2 cut(s) 94, 299
NgoMIV GCCGGC 1 cut(s) 542
NlaIV GGNNCC 1 cut(s) 547
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 1 cut(s) 505
PaeR7I CTCGAG 1 cut(s) 338
PaqCI CACCTGC 1 cut(s) 454
PdiI GCCGGC 1 cut(s) 544
PkrI GCNGC 2 cut(s) 397, 453
PleI GAGTC 2 cut(s) 70, 390
PpsI GAGTC 2 cut(s) 70, 390
Psp124BI GAGCTC 2 cut(s) 192, 430
Psp1406I AACGTT 1 cut(s) 62
Psp6I CCWGG 2 cut(s) 112, 197
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 2 cut(s) 112, 197
PspN4I GGNNCC 1 cut(s) 547
PspXI VCTCGAGB 1 cut(s) 338
PsuI RGATCY 1 cut(s) 94
SacI GAGCTC 2 cut(s) 192, 430
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 2 cut(s) 396, 452
Sau3AI GATC 2 cut(s) 94, 299
SchI GAGTC 2 cut(s) 70, 391
ScrFI CCNGG 2 cut(s) 114, 199
SduI GDGCHC 3 cut(s) 111, 192, 430
SfaNI GCATC 1 cut(s) 339
Sfr274I CTCGAG 1 cut(s) 338
SlaI CTCGAG 1 cut(s) 338
SmlI CTYRAG 2 cut(s) 191, 338
SmoI CTYRAG 2 cut(s) 191, 338
Sse9I AATT 8 cut(s) 12, 130, 221, 229, 244, 473, 636, 641
SspI AATATT 1 cut(s) 313
SspMI CTAG 1 cut(s) 98
SstI GAGCTC 2 cut(s) 192, 430
StyD4I CCNGG 2 cut(s) 112, 197
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 550
TaiI ACGT 2 cut(s) 65, 378
TaqI TCGA 2 cut(s) 339, 358
TasI AATT 8 cut(s) 12, 130, 221, 229, 244, 473, 636, 641
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 2 cut(s) 395, 451
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 3 cut(s) 59, 257, 455
TspGWI ACGGA 1 cut(s) 584
XapI RAATTY 3 cut(s) 130, 244, 636
XhoI CTCGAG 1 cut(s) 338
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.