Rroxscaffold_6G00391510

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0015869
Physical Location & Seq
Reverse (-)
9646775 .. 9649116
2342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00391510.1

Sequence Viewer

Length: 684 bp
ATGGGAAACACAATTGGAGGAAAAAGCAGGAAAGCAAAGGTGATGAGGATAAGTGGAGAAACGTTCAAGGTAAAGACTCCCATACGAGCAGGGGATGTAGTGAAGGAGCACCCAGGTCATATGCTATTGAATTCTGAAGCTGTTAAACATTTTGGTATGAGAGCAAAGCCATTAGAAGCGCAACAAGAGCTCAAGTCCAGGAGTATATATTTTCTGGTAGAATTGCCCAATTTTGATTATATGAATTCCGAAGAAGAAGCTGGAAGAAGAAGTAATAGTACGGTGGCGAGAAGGGTGAGATCGTCGGCCAATATTCAAGTGAGCGCCAAGGAGAGGCTCGAGTGCTTGATGCTGTCTCGACGCTCTGCTTCCGACCTTACAAGTCTTGTGAGAGCACCACCACCACCACCATCTTTAGGAGGAGCAGGAGGAGGAGGAGGAGGAGGAGCTCCGGTGAGGATGAAGATGAGGCTGCCGAGGGCGCAGGTGGTCAAATTGATGGAGGAGAGTAAGGATGAGATTGAAGTCGCCGACAAAATCATTCAGCTCTATATGGCCAATGCCGGCACGGTCGGCGATAACAGCAACACGGACAAAAACTTTGCTTTCAAGGCGCGTCAGAAGCGAGTGAGTTTTGTCCCCGAGAAGAGAGGAGAAATTCAATTAGATGTAGCTCCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

227

Amino Acids

24.88

Weight (kDa)

10.03

Isoelectric Point (pI)

58.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 106 3.7e-20 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015869)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66480 AT5G37840
malus_domestica MD09G1261400.v1.1
prunus_persica Prupe.3G158800_v2.0.a1
pyrus_communis pycom09g17580
rosa_chinensis RchiOBHm_Chr3g0493541
rosa_laevigata RLG00000022707
rosa_multiflora Rmu_sc0002343.1_g000048
rosa_roxburghii Rroxscaffold_6G00391510
rosa_rugosa Rorug03G0261900
rosa_samantha Rh3AG310900 Rh3BG346900 Rh3CG344800 Rh3DG346200
rosa_wichuraiana Rw3G027330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 475
Acc36I ACCTGC 1 cut(s) 475
AccII CGCG 1 cut(s) 616
AclI AACGTT 1 cut(s) 62
AcoI YGGCCR 2 cut(s) 306, 555
AcsI RAATTY 3 cut(s) 130, 244, 657
AcuI CTGAAG 1 cut(s) 156
AfaI GTAC 1 cut(s) 280
AfiI CCNNNNNNNGG 2 cut(s) 333, 416
AgsI TTSAA 6 cut(s) 67, 130, 317, 524, 610, 662
AjnI CCWGG 2 cut(s) 112, 197
AluBI AGCT 6 cut(s) 140, 190, 260, 449, 547, 674
AluI AGCT 6 cut(s) 140, 190, 260, 449, 547, 674
Alw21I GWGCWC 4 cut(s) 111, 192, 397, 451
Alw26I GTCTC 1 cut(s) 360
Ama87I CYCGRG 2 cut(s) 338, 641
AoxI GGCC 2 cut(s) 306, 555
ApeKI GCWGC 1 cut(s) 472
ApoI RAATTY 3 cut(s) 130, 244, 657
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
AspLEI GCGC 4 cut(s) 181, 326, 484, 616
AsuHPI GGTGA 3 cut(s) 52, 307, 466
AvaI CYCGRG 2 cut(s) 338, 641
BalI TGGCCA 1 cut(s) 557
BanII GRGCYC 2 cut(s) 192, 451
BarI GAAGNNNNNNTAC 2 cut(s) 262, 294
Bbv12I GWGCWC 4 cut(s) 111, 192, 397, 451
BbvI GCAGC 1 cut(s) 459
BccI CCATC 2 cut(s) 418, 493
BciT130I CCWGG 2 cut(s) 114, 199
BcoDI GTCTC 1 cut(s) 360
BfoI RGCGCY 1 cut(s) 327
BfuAI ACCTGC 1 cut(s) 475
BisI GCNGC 1 cut(s) 473
BlsI GCNGC 1 cut(s) 474
Bme1390I CCNGG 2 cut(s) 114, 199
BmeT110I CYCGRG 2 cut(s) 338, 641
BmrFI CCNGG 2 cut(s) 114, 199
BmsI GCATC 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 176
BsaJI CCNNGG 3 cut(s) 112, 327, 476
BsaWI WCCGGW 1 cut(s) 451
BsaXI ACNNNNNCTCC 2 cut(s) 438, 468
Bsc4I CCNNNNNNNGG 2 cut(s) 333, 416
Bse118I RCCGGY 1 cut(s) 563
BseBI CCWGG 2 cut(s) 114, 199
BseDI CCNNGG 3 cut(s) 112, 327, 476
BseGI GGATG 3 cut(s) 100, 465, 520
BseLI CCNNNNNNNGG 2 cut(s) 333, 416
BseXI GCAGC 1 cut(s) 459
Bsh1236I CGCG 1 cut(s) 616
Bsh1285I CGRYCG 1 cut(s) 573
BshFI GGCC 2 cut(s) 308, 557
BsiEI CGRYCG 1 cut(s) 573
BsiHKAI GWGCWC 4 cut(s) 111, 192, 397, 451
BsiHKCI CYCGRG 2 cut(s) 338, 641
BsiSI CCGG 2 cut(s) 452, 564
BslFI GGGAC 1 cut(s) 623
BslI CCNNNNNNNGG 2 cut(s) 333, 416
BsmAI GTCTC 1 cut(s) 360
BsmFI GGGAC 1 cut(s) 623
BsnI GGCC 2 cut(s) 308, 557
BsoBI CYCGRG 2 cut(s) 338, 641
Bsp1286I GDGCHC 4 cut(s) 111, 192, 397, 451
Bsp143I GATC 1 cut(s) 299
BspANI GGCC 2 cut(s) 308, 557
BspFNI CGCG 1 cut(s) 616
BspMI ACCTGC 1 cut(s) 475
BsrFI RCCGGY 1 cut(s) 563
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 3 cut(s) 112, 327, 476
BssMI GATC 1 cut(s) 299
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 2 cut(s) 114, 199
Bst4CI ACNGT 2 cut(s) 283, 571
Bst6I CTCTTC 1 cut(s) 641
BstC8I GCNNGC 1 cut(s) 565
BstF5I GGATG 3 cut(s) 100, 465, 520
BstFNI CGCG 1 cut(s) 616
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 4 cut(s) 181, 326, 484, 616
BstKTI GATC 1 cut(s) 302
BstMAI GTCTC 1 cut(s) 360
BstMBI GATC 1 cut(s) 299
BstMCI CGRYCG 1 cut(s) 573
BstMWI GCNNNNNNNGC 6 cut(s) 187, 481, 573, 582, 611, 622
BstNI CCWGG 2 cut(s) 114, 199
BstSCI CCNGG 2 cut(s) 112, 197
BstUI CGCG 1 cut(s) 616
BstV1I GCAGC 1 cut(s) 459
BsuRI GGCC 2 cut(s) 308, 557
BtsCI GGATG 3 cut(s) 100, 465, 520
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 1 cut(s) 565
CfoI GCGC 4 cut(s) 181, 326, 484, 616
Cfr10I RCCGGY 1 cut(s) 563
CseI GACGC 2 cut(s) 369, 605
Csp6I GTAC 1 cut(s) 279
CviQI GTAC 1 cut(s) 279
DpnI GATC 1 cut(s) 301
DpnII GATC 1 cut(s) 299
EaeI YGGCCR 2 cut(s) 306, 555
Eam1104I CTCTTC 1 cut(s) 641
EarI CTCTTC 1 cut(s) 641
Ecl136II GAGCTC 2 cut(s) 190, 449
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 2 cut(s) 192, 451
Eco53kI GAGCTC 2 cut(s) 190, 449
Eco57I CTGAAG 1 cut(s) 156
Eco88I CYCGRG 2 cut(s) 338, 641
EcoICRI GAGCTC 2 cut(s) 190, 449
EcoRI GAATTC 2 cut(s) 130, 244
EcoRII CCWGG 2 cut(s) 112, 197
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 2 cut(s) 192, 451
ErhI CCWWGG 1 cut(s) 327
FaqI GGGAC 1 cut(s) 623
FauNDI CATATG 1 cut(s) 120
Fnu4HI GCNGC 1 cut(s) 473
FokI GGATG 3 cut(s) 107, 472, 527
FriOI GRGCYC 2 cut(s) 192, 451
Fsp4HI GCNGC 1 cut(s) 473
GlaI GCGC 4 cut(s) 180, 325, 483, 615
GluI GCNGC 1 cut(s) 473
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 2 cut(s) 308, 557
HapII CCGG 2 cut(s) 452, 564
HgaI GACGC 2 cut(s) 369, 605
HhaI GCGC 4 cut(s) 181, 326, 484, 616
Hin6I GCGC 4 cut(s) 179, 324, 482, 614
HinP1I GCGC 4 cut(s) 179, 324, 482, 614
HinfI GANTC 1 cut(s) 76
HpaII CCGG 2 cut(s) 452, 564
HphI GGTGA 3 cut(s) 52, 307, 466
Hpy188I TCNGA 4 cut(s) 136, 250, 373, 621
Hpy188III TCNNGA 1 cut(s) 357
Hpy99I CGWCG 2 cut(s) 307, 363
HpyAV CCTTC 2 cut(s) 97, 285
HpyCH4III ACNGT 2 cut(s) 283, 571
HpyCH4IV ACGT 1 cut(s) 62
HpyF10VI GCNNNNNNNGC 6 cut(s) 187, 481, 573, 582, 611, 622
HpySE526I ACGT 1 cut(s) 62
HspAI GCGC 4 cut(s) 179, 324, 482, 614
KroI GCCGGC 1 cut(s) 563
KroNI GCCGGC 1 cut(s) 565
Kzo9I GATC 1 cut(s) 299
LmnI GCTCC 5 cut(s) 106, 422, 446, 454, 679
Lsp1109I GCAGC 1 cut(s) 459
LweI GCATC 1 cut(s) 339
MaeII ACGT 1 cut(s) 62
MalI GATC 1 cut(s) 301
MboI GATC 1 cut(s) 299
MboII GAAGA 6 cut(s) 263, 266, 276, 279, 475, 658
MfeI CAATTG 1 cut(s) 12
MhlI GDGCHC 4 cut(s) 111, 192, 397, 451
MlsI TGGCCA 1 cut(s) 557
MluCI AATT 8 cut(s) 12, 130, 221, 229, 244, 494, 657, 662
MluNI TGGCCA 1 cut(s) 557
MlyI GAGTC 1 cut(s) 70
MmeI TCCRAC 1 cut(s) 396
Mox20I TGGCCA 1 cut(s) 557
MroNI GCCGGC 1 cut(s) 563
MscI TGGCCA 1 cut(s) 557
MseI TTAA 1 cut(s) 144
Msp20I TGGCCA 1 cut(s) 557
MspI CCGG 2 cut(s) 452, 564
MspR9I CCNGG 2 cut(s) 114, 199
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 2 cut(s) 114, 199
MvnI CGCG 1 cut(s) 616
MwoI GCNNNNNNNGC 6 cut(s) 187, 481, 573, 582, 611, 622
NaeI GCCGGC 1 cut(s) 565
NdeI CATATG 1 cut(s) 120
NdeII GATC 1 cut(s) 299
NgoMIV GCCGGC 1 cut(s) 563
NmeAIII GCCGAG 1 cut(s) 501
PaeR7I CTCGAG 1 cut(s) 338
PaqCI CACCTGC 1 cut(s) 475
PdiI GCCGGC 1 cut(s) 565
PfoI TCCNGGA 1 cut(s) 197
PkrI GCNGC 1 cut(s) 474
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
Psp124BI GAGCTC 2 cut(s) 192, 451
Psp1406I AACGTT 1 cut(s) 62
Psp6I CCWGG 2 cut(s) 112, 197
PspGI CCWGG 2 cut(s) 112, 197
PspXI VCTCGAGB 1 cut(s) 338
RsaI GTAC 1 cut(s) 280
RsaNI GTAC 1 cut(s) 279
SacI GAGCTC 2 cut(s) 192, 451
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 1 cut(s) 473
Sau3AI GATC 1 cut(s) 299
SchI GAGTC 1 cut(s) 70
ScrFI CCNGG 2 cut(s) 114, 199
SduI GDGCHC 4 cut(s) 111, 192, 397, 451
SfaNI GCATC 1 cut(s) 339
Sfr274I CTCGAG 1 cut(s) 338
SlaI CTCGAG 1 cut(s) 338
SmlI CTYRAG 2 cut(s) 191, 338
SmoI CTYRAG 2 cut(s) 191, 338
Sse9I AATT 8 cut(s) 12, 130, 221, 229, 244, 494, 657, 662
SspI AATATT 1 cut(s) 313
SstI GAGCTC 2 cut(s) 192, 451
StyD4I CCNGG 2 cut(s) 112, 197
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 2 cut(s) 283, 571
TaiI ACGT 1 cut(s) 65
TaqI TCGA 2 cut(s) 339, 358
TasI AATT 8 cut(s) 12, 130, 221, 229, 244, 494, 657, 662
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseI GCWGC 1 cut(s) 472
TspDTI ATGAA 2 cut(s) 257, 476
TspGWI ACGGA 1 cut(s) 605
XapI RAATTY 3 cut(s) 130, 244, 657
XhoI CTCGAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.