Rorug03G0261900

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0015869
Physical Location & Seq
Forward (+)
25253613 .. 25259016
5404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0261900.1

Sequence Viewer

Length: 669 bp
ATGGATTATGAGCCCTTTGAAACGTGGATTTCATTTGATAAAATCATGGAGAAATGTATTTGGTTGTATGGAATAGGTGGTGTGGGGAAGAAGACACTCGTCAAAAAAGTTTATCAGGAAGCTACAAAAGATGGGAAAATTCTTGGCGATGTGGTTTTAGTACTAGATGTGAAACAAAATCCAGACCTGGAAAGAATTCAGAAAGAAATTTGTGAAACAAACGGGCTGGATGCTGAGGACAGTGAGAGCACAGTCAGTTTGAAAAGTGAGATGATGAGTGCTCCAGTGAGGCGCATAGATGAGATTCTTGCTTTACCTTATTCGGCGGATGATTTTAAGAGCGTAGATGTTCCACCTTCTGATGATGATTCTTGGCTTTACAATGGAGAGGATGAATTGAACTCTGAACTTCTACAGAGACAAAAGGAGATGAAACTTTATGATTTAAAACATAAGAAGCAGAAGAAGAAGTCAAAGAGCAGGACAATGTTGGTCCATCCTCCAGCTCCACCATTGATGGGTTCAATCCTGGGCATAAATGAAATAAATCCAAGAGCTGCAGAGATTAAAGAAAGCAATGCTCTAGCTCTTGCTATAGTTCCACATGGGATTAAACCACAATCTGGATCAAATTTGAATGATATTGGTGGGACTTCAGGTTGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

222

Amino Acids

24.82

Weight (kDa)

5.09

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SGT1 PF07093 85 - 162 3.3e-13 SGT1 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015869)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66480 AT5G37840
malus_domestica MD09G1261400.v1.1
prunus_persica Prupe.3G158800_v2.0.a1
pyrus_communis pycom09g17580
rosa_chinensis RchiOBHm_Chr3g0493541
rosa_laevigata RLG00000022707
rosa_multiflora Rmu_sc0002343.1_g000048
rosa_roxburghii Rroxscaffold_6G00391510
rosa_rugosa Rorug03G0261900
rosa_samantha Rh3AG310900 Rh3BG346900 Rh3CG344800 Rh3DG346200
rosa_wichuraiana Rw3G027330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 623
AciI CCGC 1 cut(s) 326
AclWI GGATC 1 cut(s) 634
AcsI RAATTY 4 cut(s) 138, 195, 207, 631
AcuI CTGAAG 1 cut(s) 639
AfaI GTAC 1 cut(s) 162
AfiI CCNNNNNNNGG 2 cut(s) 518, 623
AgsI TTSAA 5 cut(s) 20, 262, 400, 525, 637
AjnI CCWGG 2 cut(s) 186, 528
AluBI AGCT 4 cut(s) 122, 506, 557, 587
AluI AGCT 4 cut(s) 122, 506, 557, 587
Alw21I GWGCWC 2 cut(s) 251, 283
Alw26I GTCTC 1 cut(s) 412
AlwI GGATC 1 cut(s) 634
ApeKI GCWGC 1 cut(s) 557
ApoI RAATTY 4 cut(s) 138, 195, 207, 631
Asp700I GAANNNNTTC 1 cut(s) 195
AspLEI GCGC 1 cut(s) 294
AspS9I GGNCC 1 cut(s) 493
AvaII GGWCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 15
BbsI GAAGAC 1 cut(s) 98
Bbv12I GWGCWC 2 cut(s) 251, 283
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 544
BccI CCATC 3 cut(s) 125, 504, 511
BciT130I CCWGG 2 cut(s) 188, 530
BcoDI GTCTC 1 cut(s) 412
BfaI CTAG 2 cut(s) 164, 584
BfmI CTRYAG 3 cut(s) 413, 558, 594
BisI GCNGC 1 cut(s) 558
BlsI GCNGC 1 cut(s) 559
BmcAI AGTACT 1 cut(s) 162
Bme1390I CCNGG 2 cut(s) 188, 530
Bme18I GGWCC 1 cut(s) 493
BmgT120I GGNCC 1 cut(s) 493
BmrFI CCNGG 2 cut(s) 188, 530
BmsI GCATC 1 cut(s) 220
BoxI GACNNNNGTC 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 98
BpmI CTGGAG 2 cut(s) 267, 486
Bpu10I CCTNAGC 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 529
Bsc4I CCNNNNNNNGG 2 cut(s) 518, 623
Bse1I ACTGG 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 583
BseBI CCWGG 2 cut(s) 188, 530
BseDI CCNNGG 1 cut(s) 529
BseGI GGATG 4 cut(s) 235, 334, 397, 496
BseLI CCNNNNNNNGG 2 cut(s) 518, 623
BseMI GCAATG 1 cut(s) 583
BseMII CTCAG 1 cut(s) 225
BseNI ACTGG 1 cut(s) 284
BseXI GCAGC 1 cut(s) 544
BsiHKAI GWGCWC 2 cut(s) 251, 283
BslFI GGGAC 1 cut(s) 664
BslI CCNNNNNNNGG 2 cut(s) 518, 623
BsmAI GTCTC 1 cut(s) 412
BsmFI GGGAC 1 cut(s) 664
Bsp1286I GDGCHC 3 cut(s) 15, 251, 283
Bsp143I GATC 1 cut(s) 626
BspACI CCGC 1 cut(s) 326
BspCNI CTCAG 1 cut(s) 226
BspMAI CTGCAG 1 cut(s) 562
BspPI GGATC 1 cut(s) 634
BsrDI GCAATG 1 cut(s) 583
BsrI ACTGG 1 cut(s) 284
BssECI CCNNGG 1 cut(s) 529
BssMI GATC 1 cut(s) 626
Bst2UI CCWGG 2 cut(s) 188, 530
Bst4CI ACNGT 2 cut(s) 242, 253
BstDEI CTNAG 1 cut(s) 234
BstF5I GGATG 4 cut(s) 235, 334, 397, 496
BstHHI GCGC 1 cut(s) 294
BstKTI GATC 1 cut(s) 629
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 1 cut(s) 626
BstNI CCWGG 2 cut(s) 188, 530
BstPAI GACNNNNGTC 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 186, 528
BstSFI CTRYAG 3 cut(s) 413, 558, 594
BstV1I GCAGC 1 cut(s) 544
BstV2I GAAGAC 1 cut(s) 98
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 4 cut(s) 235, 334, 397, 496
BtsIMutI CAGTG 2 cut(s) 247, 291
CfoI GCGC 1 cut(s) 294
Cfr13I GGNCC 1 cut(s) 493
Csp6I GTAC 1 cut(s) 161
CviAII CATG 2 cut(s) 46, 605
CviJI RGCY 7 cut(s) 13, 122, 226, 376, 506, 557, 587
CviKI_1 RGCY 7 cut(s) 13, 122, 226, 376, 506, 557, 587
CviQI GTAC 1 cut(s) 161
DdeI CTNAG 1 cut(s) 234
DpnI GATC 1 cut(s) 628
DpnII GATC 1 cut(s) 626
DraI TTTAAA 1 cut(s) 447
EciI GGCGGA 1 cut(s) 341
Eco24I GRGCYC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 493
Eco57I CTGAAG 1 cut(s) 639
EcoRI GAATTC 1 cut(s) 195
EcoRII CCWGG 2 cut(s) 186, 528
EcoT38I GRGCYC 1 cut(s) 15
FaeI CATG 2 cut(s) 49, 608
FaiI YATR 9 cut(s) 9, 47, 69, 296, 441, 453, 536, 596, 606
FaqI GGGAC 1 cut(s) 664
FatI CATG 2 cut(s) 45, 604
Fnu4HI GCNGC 1 cut(s) 558
FokI GGATG 4 cut(s) 242, 341, 404, 483
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 1 cut(s) 558
FspBI CTAG 2 cut(s) 164, 584
GlaI GCGC 1 cut(s) 293
GluI GCNGC 1 cut(s) 558
GsuI CTGGAG 2 cut(s) 267, 486
HhaI GCGC 1 cut(s) 294
Hin1II CATG 2 cut(s) 49, 608
Hin6I GCGC 1 cut(s) 292
HinP1I GCGC 1 cut(s) 292
HinfI GANTC 2 cut(s) 304, 368
Hpy188I TCNGA 3 cut(s) 201, 361, 406
Hpy188III TCNNGA 3 cut(s) 116, 182, 624
HpyAV CCTTC 1 cut(s) 366
HpyCH4III ACNGT 2 cut(s) 242, 253
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 1 cut(s) 560
HpyF3I CTNAG 1 cut(s) 234
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 2 cut(s) 49, 608
HspAI GCGC 1 cut(s) 292
Kzo9I GATC 1 cut(s) 626
LmnI GCTCC 2 cut(s) 286, 511
Lsp1109I GCAGC 1 cut(s) 544
LweI GCATC 1 cut(s) 220
MaeI CTAG 2 cut(s) 164, 584
MaeII ACGT 1 cut(s) 23
MalI GATC 1 cut(s) 628
MboI GATC 1 cut(s) 626
MboII GAAGA 4 cut(s) 100, 103, 475, 478
MhlI GDGCHC 3 cut(s) 15, 251, 283
MluCI AATT 5 cut(s) 138, 195, 207, 395, 631
MnlI CCTC 4 cut(s) 229, 282, 382, 510
MroXI GAANNNNTTC 1 cut(s) 195
MseI TTAA 4 cut(s) 336, 446, 567, 612
MspR9I CCNGG 2 cut(s) 188, 530
MvaI CCWGG 2 cut(s) 188, 530
NdeII GATC 1 cut(s) 626
NlaIII CATG 2 cut(s) 49, 608
PdmI GAANNNNTTC 1 cut(s) 195
PfeI GAWTC 2 cut(s) 304, 368
PflMI CCANNNNNTGG 1 cut(s) 623
PkrI GCNGC 1 cut(s) 559
PshAI GACNNNNGTC 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 186, 528
PspGI CCWGG 2 cut(s) 186, 528
PspPI GGNCC 1 cut(s) 493
PstI CTGCAG 1 cut(s) 562
RsaI GTAC 1 cut(s) 162
RsaNI GTAC 1 cut(s) 161
SaqAI TTAA 4 cut(s) 336, 446, 567, 612
SatI GCNGC 1 cut(s) 558
Sau3AI GATC 1 cut(s) 626
Sau96I GGNCC 1 cut(s) 493
ScaI AGTACT 1 cut(s) 162
ScrFI CCNGG 2 cut(s) 188, 530
SduI GDGCHC 3 cut(s) 15, 251, 283
SfaNI GCATC 1 cut(s) 220
SfcI CTRYAG 3 cut(s) 413, 558, 594
SinI GGWCC 1 cut(s) 493
Sse9I AATT 5 cut(s) 138, 195, 207, 395, 631
SsiI CCGC 1 cut(s) 326
SspMI CTAG 2 cut(s) 164, 584
StyD4I CCNGG 2 cut(s) 186, 528
TaaI ACNGT 2 cut(s) 242, 253
TaiI ACGT 1 cut(s) 26
TasI AATT 5 cut(s) 138, 195, 207, 395, 631
TatI WGTACW 1 cut(s) 160
TfiI GAWTC 2 cut(s) 304, 368
Tru1I TTAA 4 cut(s) 336, 446, 567, 612
Tru9I TTAA 4 cut(s) 336, 446, 567, 612
TscAI CASTG 2 cut(s) 247, 291
TseI GCWGC 1 cut(s) 557
TspDTI ATGAA 4 cut(s) 21, 408, 446, 555
TspRI CASTG 2 cut(s) 247, 291
Van91I CCANNNNNTGG 1 cut(s) 623
VpaK11BI GGWCC 1 cut(s) 493
XapI RAATTY 4 cut(s) 138, 195, 207, 631
XmnI GAANNNNTTC 1 cut(s) 195
XspI CTAG 2 cut(s) 164, 584
ZrmI AGTACT 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.